Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0032430 | Msa0612000 | 0.807454 | 5.015928e-50 | -8.615850e-47 |
| Msa0079380 | Msa0612000 | 0.806217 | 9.157838e-50 | -8.615850e-47 |
| Msa0095860 | Msa0612000 | 0.820178 | 7.868359e-53 | -8.615850e-47 |
| Msa0612000 | Msa0636800 | 0.825989 | 3.470016e-54 | -8.615850e-47 |
| Msa0612000 | Msa0649110 | 0.819208 | 1.310136e-52 | -8.615850e-47 |
| Msa0612000 | Msa0765910 | 0.816252 | 6.085670e-52 | -8.615850e-47 |
| Msa0612000 | Msa0820720 | 0.947828 | 3.138597e-106 | -8.615850e-47 |
| Msa0612000 | Msa1042690 | 0.905048 | 6.475666e-80 | -8.615850e-47 |
| Msa0612000 | Msa1075650 | 0.802023 | 6.828089e-49 | -8.615850e-47 |
| Msa0612000 | Msa1181250 | 0.811097 | 8.302735e-51 | -8.615850e-47 |
| Msa0612000 | Msa1181330 | 0.804323 | 2.282962e-49 | -8.615850e-47 |
| Msa0612000 | Msa1181910 | 0.833713 | 4.552789e-56 | -8.615850e-47 |
| Msa0612000 | Msa1297160 | 0.959331 | 2.523770e-117 | -8.615850e-47 |
| Msa0612000 | Msa1412530 | 0.807660 | 4.535631e-50 | -8.615850e-47 |
| Msa0172070 | Msa0612000 | 0.807559 | 4.764193e-50 | -8.615850e-47 |
| Msa0446950 | Msa0612000 | 0.966383 | 7.568957e-126 | -8.615850e-47 |
| Msa0467380 | Msa0612000 | 0.806323 | 8.698507e-50 | -8.615850e-47 |
| Msa0475600 | Msa0612000 | 0.800353 | 1.500011e-48 | -8.615850e-47 |
| Msa0328810 | Msa0612000 | 0.807332 | 5.323249e-50 | -8.615850e-47 |
| Msa0602390 | Msa0612000 | 0.857884 | 1.204272e-62 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATACCTTTGTGAAGATAAA+AGG | 0.212182 | 4_2:-60377434 | None:intergenic |
| GTGATCTCATCCAGGATGAT+AGG | 0.356626 | 4_2:-60378179 | None:intergenic |
| CCAGGATGATAGGGTGGATA+GGG | 0.403549 | 4_2:-60378169 | None:intergenic |
| ATGTTGAAGGAGGCAAGAAC+TGG | 0.403774 | 4_2:+60378066 | Msa0612000:CDS |
| TCTTATAAAATCATCATGAT+AGG | 0.434799 | 4_2:-60378241 | None:intergenic |
| GCAAGAGGTGTGATGGCAGC+AGG | 0.439246 | 4_2:-60378110 | None:intergenic |
| TGATGGCAGCAGGGGTAAGT+TGG | 0.456592 | 4_2:-60378100 | None:intergenic |
| CATACTACCTCTGCTAAGAT+TGG | 0.469905 | 4_2:+60378134 | Msa0612000:CDS |
| CCCTATCCACCCTATCATCC+TGG | 0.477480 | 4_2:+60378169 | Msa0612000:CDS |
| ACCATGTTGCAGAATGTTGA+AGG | 0.481802 | 4_2:+60378053 | Msa0612000:CDS |
| ATTCTGCAACATGGTGGAAG+GGG | 0.513075 | 4_2:-60378045 | None:intergenic |
| TTGCAGGAGTGATCTCATCC+AGG | 0.524797 | 4_2:-60378187 | None:intergenic |
| TGTCTTTGAACAACAAGTGC+AGG | 0.532648 | 4_2:+60377400 | Msa0612000:CDS |
| AAAATCATCATGATAGGAAG+AGG | 0.535692 | 4_2:-60378235 | None:intergenic |
| ACATTCTGCAACATGGTGGA+AGG | 0.539324 | 4_2:-60378047 | None:intergenic |
| TGATCTCATCCAGGATGATA+GGG | 0.550931 | 4_2:-60378178 | None:intergenic |
| TCCTTCAACATTCTGCAACA+TGG | 0.556193 | 4_2:-60378054 | None:intergenic |
| CATTCTGCAACATGGTGGAA+GGG | 0.560478 | 4_2:-60378046 | None:intergenic |
| AGTATGAGCAAGAGGTGTGA+TGG | 0.566077 | 4_2:-60378117 | None:intergenic |
| TCCAGGATGATAGGGTGGAT+AGG | 0.585429 | 4_2:-60378170 | None:intergenic |
| CAAGAGGTGTGATGGCAGCA+GGG | 0.589536 | 4_2:-60378109 | None:intergenic |
| GCAGAGGTAGTATGAGCAAG+AGG | 0.599567 | 4_2:-60378125 | None:intergenic |
| TGTTGAAGGAGGCAAGAACT+GGG | 0.613431 | 4_2:+60378067 | Msa0612000:CDS |
| CAGGATGATAGGGTGGATAG+GGG | 0.620934 | 4_2:-60378168 | None:intergenic |
| AAGAGGTGTGATGGCAGCAG+GGG | 0.623171 | 4_2:-60378108 | None:intergenic |
| TTCAACATTCTGCAACATGG+TGG | 0.658953 | 4_2:-60378051 | None:intergenic |
| TCTCATCCAGGATGATAGGG+TGG | 0.663515 | 4_2:-60378175 | None:intergenic |
| ACTTGAACCAATCTTAGCAG+AGG | 0.665354 | 4_2:-60378141 | None:intergenic |
| ATGTTGCAGAATGTTGAAGG+AGG | 0.685850 | 4_2:+60378056 | Msa0612000:CDS |
| TATGATTTAGGACACAAATG+TGG | 0.703373 | 4_2:+60377827 | Msa0612000:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAACTTATAACATGTTAAA+TGG | + | chr4_2:60377517-60377536 | Msa0612000:intron | 20.0% |
| !! | ATGTTATTATGCAGTTATTT+AGG | - | chr4_2:60377564-60377583 | None:intergenic | 20.0% |
| !! | TAAAAAAAAATAGCAGTTGA+TGG | - | chr4_2:60377602-60377621 | None:intergenic | 20.0% |
| !! | ACATATGTTTAAACATATCA+AGG | - | chr4_2:60377962-60377981 | None:intergenic | 20.0% |
| !! | CAAACTTATAACATGTTAAA+TGG | + | chr4_2:60377517-60377536 | Msa0612000:intron | 20.0% |
| !! | ATGTTATTATGCAGTTATTT+AGG | - | chr4_2:60377564-60377583 | None:intergenic | 20.0% |
| !! | TAAAAAAAAATAGCAGTTGA+TGG | - | chr4_2:60377602-60377621 | None:intergenic | 20.0% |
| !! | ACATATGTTTAAACATATCA+AGG | - | chr4_2:60377962-60377981 | None:intergenic | 20.0% |
| !! | TTTAGGATATGTACATATGA+AGG | - | chr4_2:60377547-60377566 | None:intergenic | 25.0% |
| ! | TAAGATAGTAACATATCCTA+AGG | + | chr4_2:60377620-60377639 | Msa0612000:intron | 25.0% |
| ! | AAGATGTCATAAATTAGTCA+AGG | - | chr4_2:60377723-60377742 | None:intergenic | 25.0% |
| ! | TCAATTAAGATGATCTACAT+TGG | + | chr4_2:60377893-60377912 | Msa0612000:intron | 25.0% |
| !! | AAAAAACTCTTACTCTAAGA+TGG | + | chr4_2:60378208-60378227 | Msa0612000:CDS | 25.0% |
| !! | TTTAGGATATGTACATATGA+AGG | - | chr4_2:60377547-60377566 | None:intergenic | 25.0% |
| ! | TAAGATAGTAACATATCCTA+AGG | + | chr4_2:60377620-60377639 | Msa0612000:intron | 25.0% |
| ! | AAGATGTCATAAATTAGTCA+AGG | - | chr4_2:60377723-60377742 | None:intergenic | 25.0% |
| ! | TCAATTAAGATGATCTACAT+TGG | + | chr4_2:60377893-60377912 | Msa0612000:intron | 25.0% |
| !! | AAAAAACTCTTACTCTAAGA+TGG | + | chr4_2:60378208-60378227 | Msa0612000:CDS | 25.0% |
| ! | TACTCCTTTTATCTTCACAA+AGG | + | chr4_2:60377430-60377449 | Msa0612000:CDS | 30.0% |
| CATACCTTTGTGAAGATAAA+AGG | - | chr4_2:60377437-60377456 | None:intergenic | 30.0% | |
| ATAGTAACATATCCTAAGGA+CGG | + | chr4_2:60377624-60377643 | Msa0612000:intron | 30.0% | |
| TCAAGGAAATAATCAGTTGT+AGG | - | chr4_2:60377706-60377725 | None:intergenic | 30.0% | |
| TGTGTGTTTGTTTGCTTATT+AGG | + | chr4_2:60377746-60377765 | Msa0612000:intron | 30.0% | |
| GTGTGTTTGTTTGCTTATTA+GGG | + | chr4_2:60377747-60377766 | Msa0612000:intron | 30.0% | |
| ! | TATGATTTAGGACACAAATG+TGG | + | chr4_2:60377827-60377846 | Msa0612000:intron | 30.0% |
| ! | CTACATTGGTTATTTATCTG+CGG | + | chr4_2:60377907-60377926 | Msa0612000:intron | 30.0% |
| !!! | TTAGAGTAAGAGTTTTTTGC+AGG | - | chr4_2:60378206-60378225 | None:intergenic | 30.0% |
| ! | TACTCCTTTTATCTTCACAA+AGG | + | chr4_2:60377430-60377449 | Msa0612000:CDS | 30.0% |
| CATACCTTTGTGAAGATAAA+AGG | - | chr4_2:60377437-60377456 | None:intergenic | 30.0% | |
| ATAGTAACATATCCTAAGGA+CGG | + | chr4_2:60377624-60377643 | Msa0612000:intron | 30.0% | |
| TCAAGGAAATAATCAGTTGT+AGG | - | chr4_2:60377706-60377725 | None:intergenic | 30.0% | |
| TGTGTGTTTGTTTGCTTATT+AGG | + | chr4_2:60377746-60377765 | Msa0612000:intron | 30.0% | |
| GTGTGTTTGTTTGCTTATTA+GGG | + | chr4_2:60377747-60377766 | Msa0612000:intron | 30.0% | |
| ! | TATGATTTAGGACACAAATG+TGG | + | chr4_2:60377827-60377846 | Msa0612000:intron | 30.0% |
| ! | CTACATTGGTTATTTATCTG+CGG | + | chr4_2:60377907-60377926 | Msa0612000:intron | 30.0% |
| !!! | TTAGAGTAAGAGTTTTTTGC+AGG | - | chr4_2:60378206-60378225 | None:intergenic | 30.0% |
| !! | CAGTTGATGGATATGTACTT+AGG | - | chr4_2:60377589-60377608 | None:intergenic | 35.0% |
| TAGTAACATATCCTAAGGAC+GGG | + | chr4_2:60377625-60377644 | Msa0612000:intron | 35.0% | |
| AGATGACGATGCTATGATTT+AGG | + | chr4_2:60377815-60377834 | Msa0612000:intron | 35.0% | |
| !!! | CAAGGTTAGTGTTTTGTTTC+AGG | - | chr4_2:60377944-60377963 | None:intergenic | 35.0% |
| TAAGAACATGATCTGTCACT+TGG | + | chr4_2:60378011-60378030 | Msa0612000:intron | 35.0% | |
| !! | CAGTTGATGGATATGTACTT+AGG | - | chr4_2:60377589-60377608 | None:intergenic | 35.0% |
| TAGTAACATATCCTAAGGAC+GGG | + | chr4_2:60377625-60377644 | Msa0612000:intron | 35.0% | |
| AGATGACGATGCTATGATTT+AGG | + | chr4_2:60377815-60377834 | Msa0612000:intron | 35.0% | |
| !!! | CAAGGTTAGTGTTTTGTTTC+AGG | - | chr4_2:60377944-60377963 | None:intergenic | 35.0% |
| TAAGAACATGATCTGTCACT+TGG | + | chr4_2:60378011-60378030 | Msa0612000:intron | 35.0% | |
| TGTCTTTGAACAACAAGTGC+AGG | + | chr4_2:60377400-60377419 | Msa0612000:CDS | 40.0% | |
| GATGGTCATTGTTGATGCTA+AGG | + | chr4_2:60377662-60377681 | Msa0612000:intron | 40.0% | |
| ATCATAGCATCGTCATCTTG+TGG | - | chr4_2:60377813-60377832 | None:intergenic | 40.0% | |
| TTCAACATTCTGCAACATGG+TGG | - | chr4_2:60378054-60378073 | None:intergenic | 40.0% | |
| ACCATGTTGCAGAATGTTGA+AGG | + | chr4_2:60378053-60378072 | Msa0612000:CDS | 40.0% | |
| TCCTTCAACATTCTGCAACA+TGG | - | chr4_2:60378057-60378076 | None:intergenic | 40.0% | |
| ATGTTGCAGAATGTTGAAGG+AGG | + | chr4_2:60378056-60378075 | Msa0612000:CDS | 40.0% | |
| CATACTACCTCTGCTAAGAT+TGG | + | chr4_2:60378134-60378153 | Msa0612000:CDS | 40.0% | |
| ACTTGAACCAATCTTAGCAG+AGG | - | chr4_2:60378144-60378163 | None:intergenic | 40.0% | |
| TGATCTCATCCAGGATGATA+GGG | - | chr4_2:60378181-60378200 | None:intergenic | 40.0% | |
| TGTCTTTGAACAACAAGTGC+AGG | + | chr4_2:60377400-60377419 | Msa0612000:CDS | 40.0% | |
| GATGGTCATTGTTGATGCTA+AGG | + | chr4_2:60377662-60377681 | Msa0612000:intron | 40.0% | |
| ATCATAGCATCGTCATCTTG+TGG | - | chr4_2:60377813-60377832 | None:intergenic | 40.0% | |
| TTCAACATTCTGCAACATGG+TGG | - | chr4_2:60378054-60378073 | None:intergenic | 40.0% | |
| ACCATGTTGCAGAATGTTGA+AGG | + | chr4_2:60378053-60378072 | Msa0612000:CDS | 40.0% | |
| TCCTTCAACATTCTGCAACA+TGG | - | chr4_2:60378057-60378076 | None:intergenic | 40.0% | |
| ATGTTGCAGAATGTTGAAGG+AGG | + | chr4_2:60378056-60378075 | Msa0612000:CDS | 40.0% | |
| CATACTACCTCTGCTAAGAT+TGG | + | chr4_2:60378134-60378153 | Msa0612000:CDS | 40.0% | |
| ACTTGAACCAATCTTAGCAG+AGG | - | chr4_2:60378144-60378163 | None:intergenic | 40.0% | |
| TGATCTCATCCAGGATGATA+GGG | - | chr4_2:60378181-60378200 | None:intergenic | 40.0% | |
| AACATATCCTAAGGACGGGA+AGG | + | chr4_2:60377629-60377648 | Msa0612000:intron | 45.0% | |
| AAATGTTCCTTCCCGTCCTT+AGG | - | chr4_2:60377639-60377658 | None:intergenic | 45.0% | |
| TAAGGACGGGAAGGAACATT+TGG | + | chr4_2:60377638-60377657 | Msa0612000:intron | 45.0% | |
| ! | ATTCTGCAACATGGTGGAAG+GGG | - | chr4_2:60378048-60378067 | None:intergenic | 45.0% |
| ! | CATTCTGCAACATGGTGGAA+GGG | - | chr4_2:60378049-60378068 | None:intergenic | 45.0% |
| ! | ACATTCTGCAACATGGTGGA+AGG | - | chr4_2:60378050-60378069 | None:intergenic | 45.0% |
| ATGTTGAAGGAGGCAAGAAC+TGG | + | chr4_2:60378066-60378085 | Msa0612000:CDS | 45.0% | |
| TGTTGAAGGAGGCAAGAACT+GGG | + | chr4_2:60378067-60378086 | Msa0612000:CDS | 45.0% | |
| ! | AGTATGAGCAAGAGGTGTGA+TGG | - | chr4_2:60378120-60378139 | None:intergenic | 45.0% |
| GTGATCTCATCCAGGATGAT+AGG | - | chr4_2:60378182-60378201 | None:intergenic | 45.0% | |
| AACATATCCTAAGGACGGGA+AGG | + | chr4_2:60377629-60377648 | Msa0612000:intron | 45.0% | |
| AAATGTTCCTTCCCGTCCTT+AGG | - | chr4_2:60377639-60377658 | None:intergenic | 45.0% | |
| TAAGGACGGGAAGGAACATT+TGG | + | chr4_2:60377638-60377657 | Msa0612000:intron | 45.0% | |
| ! | ATTCTGCAACATGGTGGAAG+GGG | - | chr4_2:60378048-60378067 | None:intergenic | 45.0% |
| ! | CATTCTGCAACATGGTGGAA+GGG | - | chr4_2:60378049-60378068 | None:intergenic | 45.0% |
| ! | ACATTCTGCAACATGGTGGA+AGG | - | chr4_2:60378050-60378069 | None:intergenic | 45.0% |
| ATGTTGAAGGAGGCAAGAAC+TGG | + | chr4_2:60378066-60378085 | Msa0612000:CDS | 45.0% | |
| TGTTGAAGGAGGCAAGAACT+GGG | + | chr4_2:60378067-60378086 | Msa0612000:CDS | 45.0% | |
| ! | AGTATGAGCAAGAGGTGTGA+TGG | - | chr4_2:60378120-60378139 | None:intergenic | 45.0% |
| GTGATCTCATCCAGGATGAT+AGG | - | chr4_2:60378182-60378201 | None:intergenic | 45.0% | |
| CGGGAAGGAACATTTGGAGA+TGG | + | chr4_2:60377644-60377663 | Msa0612000:intron | 50.0% | |
| AAGTTGCAGATGCAGCACAC+TGG | - | chr4_2:60377986-60378005 | None:intergenic | 50.0% | |
| GCAGAGGTAGTATGAGCAAG+AGG | - | chr4_2:60378128-60378147 | None:intergenic | 50.0% | |
| CAGGATGATAGGGTGGATAG+GGG | - | chr4_2:60378171-60378190 | None:intergenic | 50.0% | |
| CCAGGATGATAGGGTGGATA+GGG | - | chr4_2:60378172-60378191 | None:intergenic | 50.0% | |
| TCCAGGATGATAGGGTGGAT+AGG | - | chr4_2:60378173-60378192 | None:intergenic | 50.0% | |
| TCTCATCCAGGATGATAGGG+TGG | - | chr4_2:60378178-60378197 | None:intergenic | 50.0% | |
| TTGCAGGAGTGATCTCATCC+AGG | - | chr4_2:60378190-60378209 | None:intergenic | 50.0% | |
| CGGGAAGGAACATTTGGAGA+TGG | + | chr4_2:60377644-60377663 | Msa0612000:intron | 50.0% | |
| AAGTTGCAGATGCAGCACAC+TGG | - | chr4_2:60377986-60378005 | None:intergenic | 50.0% | |
| GCAGAGGTAGTATGAGCAAG+AGG | - | chr4_2:60378128-60378147 | None:intergenic | 50.0% | |
| CAGGATGATAGGGTGGATAG+GGG | - | chr4_2:60378171-60378190 | None:intergenic | 50.0% | |
| CCAGGATGATAGGGTGGATA+GGG | - | chr4_2:60378172-60378191 | None:intergenic | 50.0% | |
| TCCAGGATGATAGGGTGGAT+AGG | - | chr4_2:60378173-60378192 | None:intergenic | 50.0% | |
| TCTCATCCAGGATGATAGGG+TGG | - | chr4_2:60378178-60378197 | None:intergenic | 50.0% | |
| TTGCAGGAGTGATCTCATCC+AGG | - | chr4_2:60378190-60378209 | None:intergenic | 50.0% | |
| TGATGGCAGCAGGGGTAAGT+TGG | - | chr4_2:60378103-60378122 | None:intergenic | 55.0% | |
| ! | AAGAGGTGTGATGGCAGCAG+GGG | - | chr4_2:60378111-60378130 | None:intergenic | 55.0% |
| ! | CAAGAGGTGTGATGGCAGCA+GGG | - | chr4_2:60378112-60378131 | None:intergenic | 55.0% |
| CCCTATCCACCCTATCATCC+TGG | + | chr4_2:60378169-60378188 | Msa0612000:CDS | 55.0% | |
| TGATGGCAGCAGGGGTAAGT+TGG | - | chr4_2:60378103-60378122 | None:intergenic | 55.0% | |
| ! | AAGAGGTGTGATGGCAGCAG+GGG | - | chr4_2:60378111-60378130 | None:intergenic | 55.0% |
| ! | CAAGAGGTGTGATGGCAGCA+GGG | - | chr4_2:60378112-60378131 | None:intergenic | 55.0% |
| CCCTATCCACCCTATCATCC+TGG | + | chr4_2:60378169-60378188 | Msa0612000:CDS | 55.0% | |
| ! | GCAAGAGGTGTGATGGCAGC+AGG | - | chr4_2:60378113-60378132 | None:intergenic | 60.0% |
| ! | GCAAGAGGTGTGATGGCAGC+AGG | - | chr4_2:60378113-60378132 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 60377392 | 60378250 | 60377392 | ID=Msa0612000;Name=Msa0612000 |
| chr4_2 | mRNA | 60377392 | 60378250 | 60377392 | ID=Msa0612000-mRNA-1;Parent=Msa0612000;Name=Msa0612000-mRNA-1;_AED=0.42;_eAED=0.81;_QI=0|0|0|1|0.5|0.66|3|0|103 |
| chr4_2 | exon | 60377392 | 60377451 | 60377392 | ID=Msa0612000-mRNA-1:exon:16199;Parent=Msa0612000-mRNA-1 |
| chr4_2 | exon | 60377837 | 60377875 | 60377837 | ID=Msa0612000-mRNA-1:exon:16200;Parent=Msa0612000-mRNA-1 |
| chr4_2 | exon | 60378038 | 60378250 | 60378038 | ID=Msa0612000-mRNA-1:exon:16201;Parent=Msa0612000-mRNA-1 |
| chr4_2 | CDS | 60377392 | 60377451 | 60377392 | ID=Msa0612000-mRNA-1:cds;Parent=Msa0612000-mRNA-1 |
| chr4_2 | CDS | 60377837 | 60377875 | 60377837 | ID=Msa0612000-mRNA-1:cds;Parent=Msa0612000-mRNA-1 |
| chr4_2 | CDS | 60378038 | 60378250 | 60378038 | ID=Msa0612000-mRNA-1:cds;Parent=Msa0612000-mRNA-1 |
| Gene Sequence |
| Protein sequence |
