Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0644060 | AES88834.2 | 75.758 | 99 | 14 | 3 | 1 | 98 | 1 | 90 | 5.99e-30 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0092390 | Msa0644060 | 0.831565 | 1.553043e-55 | -8.615850e-47 |
| Msa0110350 | Msa0644060 | 0.801906 | 7.217024e-49 | -8.615850e-47 |
| Msa0644060 | Msa0655360 | 0.813268 | 2.788877e-51 | -8.615850e-47 |
| Msa0644060 | Msa0691150 | 0.818707 | 1.703552e-52 | -8.615850e-47 |
| Msa0644060 | Msa0807170 | 0.802973 | 4.350581e-49 | -8.615850e-47 |
| Msa0644060 | Msa0902810 | 0.832700 | 8.139229e-56 | -8.615850e-47 |
| Msa0644060 | Msa1158540 | 0.802885 | 4.535987e-49 | -8.615850e-47 |
| Msa0644060 | Msa1377980 | 0.800090 | 1.696336e-48 | -8.615850e-47 |
| Msa0198870 | Msa0644060 | 0.803516 | 3.358712e-49 | -8.615850e-47 |
| Msa0207150 | Msa0644060 | 0.826863 | 2.147710e-54 | -8.615850e-47 |
| Msa0440220 | Msa0644060 | 0.823003 | 1.749847e-53 | -8.615850e-47 |
| Msa0274890 | Msa0644060 | 0.800570 | 1.354835e-48 | -8.615850e-47 |
| Msa0279910 | Msa0644060 | 0.823227 | 1.551872e-53 | -8.615850e-47 |
| Msa0323790 | Msa0644060 | 0.801542 | 8.574689e-49 | -8.615850e-47 |
| Msa0521190 | Msa0644060 | 0.804463 | 2.134384e-49 | -8.615850e-47 |
| Msa0559280 | Msa0644060 | 0.838390 | 2.959282e-57 | -8.615850e-47 |
| Msa0562680 | Msa0644060 | 0.818631 | 1.772708e-52 | -8.615850e-47 |
| Msa0575970 | Msa0644060 | 0.815074 | 1.113889e-51 | -8.615850e-47 |
| Msa0576110 | Msa0644060 | 0.804004 | 2.659682e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0644060 | MtrunA17_Chr4g0031021 | 75.758 | 99 | 14 | 3 | 1 | 98 | 1 | 90 | 5.51e-34 | 113 |
| Msa0644060 | MtrunA17_Chr4g0031011 | 51.064 | 94 | 45 | 1 | 1 | 94 | 1 | 93 | 2.00e-14 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGGTCTGGGTGAGAAGTT+TGG | 0.374289 | 4_3:+47634419 | Msa0644060:CDS |
| AAGGCAATGTCATGTGAGTA+TGG | 0.402722 | 4_3:-47634358 | None:intergenic |
| CAACACTAAGGTCATGGCTT+TGG | 0.441416 | 4_3:-47634255 | None:intergenic |
| ATGATGTAAACGACGTTGCT+AGG | 0.472531 | 4_3:+47634501 | Msa0644060:CDS |
| CACTACGGTGCTTTCTGAAG+AGG | 0.485776 | 4_3:+47634304 | Msa0644060:CDS |
| TCCACCGGTAGACGCATGCT+AGG | 0.507807 | 4_3:+47634380 | Msa0644060:CDS |
| GAACCCTAGCATGCGTCTAC+CGG | 0.513042 | 4_3:-47634384 | None:intergenic |
| CATTCAATCATTGTTATCAT+CGG | 0.520776 | 4_3:-47634531 | None:intergenic |
| TGGGTAGGAGAGTAAGTGGT+GGG | 0.540063 | 4_3:-47634338 | None:intergenic |
| TCTACAGTTGAATCAAACTT+CGG | 0.549994 | 4_3:-47634443 | None:intergenic |
| TGATGTAAACGACGTTGCTA+GGG | 0.561947 | 4_3:+47634502 | Msa0644060:CDS |
| CACATGACATTGCCTTCCAC+CGG | 0.580768 | 4_3:+47634365 | Msa0644060:CDS |
| GATTCAACTGTAGAAAAGCA+TGG | 0.589665 | 4_3:+47634452 | Msa0644060:CDS |
| CCCTAGCATGCGTCTACCGG+TGG | 0.591972 | 4_3:-47634381 | None:intergenic |
| GAGGAGCAACACTAAGGTCA+TGG | 0.596052 | 4_3:-47634261 | None:intergenic |
| TAGGAGGAGGAGCAACACTA+AGG | 0.598370 | 4_3:-47634267 | None:intergenic |
| CCACCGGTAGACGCATGCTA+GGG | 0.606573 | 4_3:+47634381 | Msa0644060:CDS |
| TAGTGAAAAGAGACAAAACT+AGG | 0.608337 | 4_3:-47634286 | None:intergenic |
| AGTATGGGTAGGAGAGTAAG+TGG | 0.611157 | 4_3:-47634342 | None:intergenic |
| AGCATGCGTCTACCGGTGGA+AGG | 0.612142 | 4_3:-47634377 | None:intergenic |
| AAAGAGACAAAACTAGGAGG+AGG | 0.615522 | 4_3:-47634280 | None:intergenic |
| AATGTCATGTGAGTATGGGT+AGG | 0.634885 | 4_3:-47634353 | None:intergenic |
| ATGGGTAGGAGAGTAAGTGG+TGG | 0.647747 | 4_3:-47634339 | None:intergenic |
| AGGCAATGTCATGTGAGTAT+GGG | 0.653460 | 4_3:-47634357 | None:intergenic |
| TGAAAAGAGACAAAACTAGG+AGG | 0.694756 | 4_3:-47634283 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAGTGAAAAGAGACAAAACT+AGG | - | chr4_3:47634289-47634308 | None:intergenic | 30.0% | |
| !! | AGTTTTGTCTCTTTTCACTA+CGG | + | chr4_3:47634289-47634308 | Msa0644060:CDS | 30.0% |
| TCTACAGTTGAATCAAACTT+CGG | - | chr4_3:47634446-47634465 | None:intergenic | 30.0% | |
| TGAAAAGAGACAAAACTAGG+AGG | - | chr4_3:47634286-47634305 | None:intergenic | 35.0% | |
| GATTCAACTGTAGAAAAGCA+TGG | + | chr4_3:47634452-47634471 | Msa0644060:CDS | 35.0% | |
| AAAGAGACAAAACTAGGAGG+AGG | - | chr4_3:47634283-47634302 | None:intergenic | 40.0% | |
| AATGTCATGTGAGTATGGGT+AGG | - | chr4_3:47634356-47634375 | None:intergenic | 40.0% | |
| AGGCAATGTCATGTGAGTAT+GGG | - | chr4_3:47634360-47634379 | None:intergenic | 40.0% | |
| AAGGCAATGTCATGTGAGTA+TGG | - | chr4_3:47634361-47634380 | None:intergenic | 40.0% | |
| ATGATGTAAACGACGTTGCT+AGG | + | chr4_3:47634501-47634520 | Msa0644060:CDS | 40.0% | |
| TGATGTAAACGACGTTGCTA+GGG | + | chr4_3:47634502-47634521 | Msa0644060:CDS | 40.0% | |
| CAACACTAAGGTCATGGCTT+TGG | - | chr4_3:47634258-47634277 | None:intergenic | 45.0% | |
| AGTATGGGTAGGAGAGTAAG+TGG | - | chr4_3:47634345-47634364 | None:intergenic | 45.0% | |
| GAGGAGCAACACTAAGGTCA+TGG | - | chr4_3:47634264-47634283 | None:intergenic | 50.0% | |
| TAGGAGGAGGAGCAACACTA+AGG | - | chr4_3:47634270-47634289 | None:intergenic | 50.0% | |
| ! | CACTACGGTGCTTTCTGAAG+AGG | + | chr4_3:47634304-47634323 | Msa0644060:CDS | 50.0% |
| TGGGTAGGAGAGTAAGTGGT+GGG | - | chr4_3:47634341-47634360 | None:intergenic | 50.0% | |
| ATGGGTAGGAGAGTAAGTGG+TGG | - | chr4_3:47634342-47634361 | None:intergenic | 50.0% | |
| CACATGACATTGCCTTCCAC+CGG | + | chr4_3:47634365-47634384 | Msa0644060:CDS | 50.0% | |
| !!! | AGGGTTCGCCAGCTTTTTCA+AGG | + | chr4_3:47634400-47634419 | Msa0644060:CDS | 50.0% |
| !!! | GGGTTCGCCAGCTTTTTCAA+GGG | + | chr4_3:47634401-47634420 | Msa0644060:CDS | 50.0% |
| !!! | GCCAGCTTTTTCAAGGGTCT+GGG | + | chr4_3:47634407-47634426 | Msa0644060:CDS | 50.0% |
| ACCCAGACCCTTGAAAAAGC+TGG | - | chr4_3:47634411-47634430 | None:intergenic | 50.0% | |
| AAGGGTCTGGGTGAGAAGTT+TGG | + | chr4_3:47634419-47634438 | Msa0644060:CDS | 50.0% | |
| GAACCCTAGCATGCGTCTAC+CGG | - | chr4_3:47634387-47634406 | None:intergenic | 55.0% | |
| !!! | CGCCAGCTTTTTCAAGGGTC+TGG | + | chr4_3:47634406-47634425 | Msa0644060:CDS | 55.0% |
| AGCATGCGTCTACCGGTGGA+AGG | - | chr4_3:47634380-47634399 | None:intergenic | 60.0% | |
| TCCACCGGTAGACGCATGCT+AGG | + | chr4_3:47634380-47634399 | Msa0644060:CDS | 60.0% | |
| CCACCGGTAGACGCATGCTA+GGG | + | chr4_3:47634381-47634400 | Msa0644060:CDS | 60.0% | |
| CCCTAGCATGCGTCTACCGG+TGG | - | chr4_3:47634384-47634403 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 47634251 | 47634550 | 47634251 | ID=Msa0644060;Name=Msa0644060 |
| chr4_3 | mRNA | 47634251 | 47634550 | 47634251 | ID=Msa0644060-mRNA-1;Parent=Msa0644060;Name=Msa0644060-mRNA-1;_AED=0.25;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|99 |
| chr4_3 | exon | 47634251 | 47634550 | 47634251 | ID=Msa0644060-mRNA-1:exon:11288;Parent=Msa0644060-mRNA-1 |
| chr4_3 | CDS | 47634251 | 47634550 | 47634251 | ID=Msa0644060-mRNA-1:cds;Parent=Msa0644060-mRNA-1 |
| Gene Sequence |
| Protein sequence |
