AlfalfaGEDB | Msa0688490

M. sativa cultivar zhongmu-4 / Msa0688490

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02701.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0688490	XP_013456040.2	98.616	289	4	0	1	289	1	289	0.0	596

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0688490	sp\|Q8LE43\|DOF21_ARATH	41.083	314	122	11	10	289	4	288	1.15e-53	179

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0688490	A0A396I5B7	98.616	289	4	0	1	289	1	289	0.0	596

TFs/TRs:

Gene ID	Type	Classification
Msa0688490	TF	C2C2-Dof

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000050	Msa0688490	0.809479	1.853768e-50	-8.615850e-47
Msa0002580	Msa0688490	0.804886	1.742071e-49	-8.615850e-47
Msa0688490	Msa0833530	0.820902	5.366884e-53	-8.615850e-47
Msa0688490	Msa0945550	0.842137	3.106166e-58	-8.615850e-47
Msa0688490	Msa1101090	0.820758	5.790868e-53	-8.615850e-47
Msa0688490	Msa1228200	0.800601	1.334999e-48	-8.615850e-47
Msa0688490	Msa1265650	0.806519	7.908420e-50	-8.615850e-47
Msa0688490	Msa1425880	0.812404	4.313701e-51	-8.615850e-47
Msa0688490	Msa1444510	0.806261	8.963684e-50	-8.615850e-47
Msa0172650	Msa0688490	0.837777	4.255858e-57	-8.615850e-47
Msa0189040	Msa0688490	0.802302	5.983766e-49	-8.615850e-47
Msa0221470	Msa0688490	0.821569	3.765409e-53	-8.615850e-47
Msa0221500	Msa0688490	0.822365	2.463687e-53	-8.615850e-47
Msa0376630	Msa0688490	0.816650	4.958245e-52	-8.615850e-47
Msa0406770	Msa0688490	0.808959	2.396849e-50	-8.615850e-47
Msa0428920	Msa0688490	0.808420	3.124808e-50	-8.615850e-47
Msa0434290	Msa0688490	0.810567	1.080906e-50	-8.615850e-47
Msa0244640	Msa0688490	0.808452	3.075287e-50	-8.615850e-47
Msa0263060	Msa0688490	0.828875	7.050263e-55	-8.615850e-47
Msa0283330	Msa0688490	0.810009	1.426454e-50	-8.615850e-47
Msa0321620	Msa0688490	0.845544	3.800184e-59	-8.615850e-47
Msa0360210	Msa0688490	0.804830	1.788968e-49	-8.615850e-47
Msa0522150	Msa0688490	0.809268	2.057817e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0688490	MtrunA17_Chr4g0030051	98.616	289	4	0	1	289	1	289	0.0	596
Msa0688490	MtrunA17_Chr2g0326001	54.817	301	81	15	19	289	18	293	5.86e-85	256
Msa0688490	MtrunA17_Chr3g0117601	34.718	337	141	15	2	289	28	334	1.49e-35	130
Msa0688490	MtrunA17_Chr3g0127201	76.471	68	16	0	30	97	16	83	5.68e-34	125
Msa0688490	MtrunA17_Chr4g0031411	66.304	92	26	2	25	111	58	149	1.08e-33	125
Msa0688490	MtrunA17_Chr1g0188851	56.701	97	38	1	3	95	1	97	1.35e-33	124
Msa0688490	MtrunA17_Chr8g0341671	70.270	74	22	0	29	102	8	81	4.42e-32	118
Msa0688490	MtrunA17_Chr3g0126201	64.706	85	30	0	15	99	25	109	5.14e-32	120
Msa0688490	MtrunA17_Chr2g0328021	70.423	71	20	1	26	96	48	117	9.56e-32	119
Msa0688490	MtrunA17_Chr4g0010151	66.250	80	26	1	17	95	57	136	1.04e-31	120
Msa0688490	MtrunA17_Chr4g0047001	59.596	99	30	2	42	130	56	154	1.19e-31	120
Msa0688490	MtrunA17_Chr8g0346551	66.234	77	24	1	23	99	8	82	2.04e-31	117
Msa0688490	MtrunA17_Chr7g0237921	68.831	77	23	1	20	95	54	130	7.04e-31	118
Msa0688490	MtrunA17_Chr1g0177251	67.949	78	22	1	43	117	14	91	8.85e-31	116
Msa0688490	MtrunA17_Chr2g0282441	85.965	57	6	1	36	92	22	76	9.66e-31	117
Msa0688490	MtrunA17_Chr8g0374251	82.143	56	10	0	39	94	66	121	1.52e-30	117
Msa0688490	MtrunA17_Chr8g0367161	61.538	78	30	0	37	114	28	105	1.94e-30	117
Msa0688490	MtrunA17_Chr7g0223311	72.857	70	18	1	40	108	76	145	1.77e-29	115
Msa0688490	MtrunA17_Chr5g0411951	80.702	57	10	1	35	91	38	93	3.06e-29	113
Msa0688490	MtrunA17_Chr8g0374111	70.588	68	18	1	32	97	18	85	3.44e-29	110
Msa0688490	MtrunA17_Chr4g0061761	86.792	53	7	0	39	91	38	90	3.53e-29	113
Msa0688490	MtrunA17_Chr2g0282511	90.000	50	5	0	40	89	44	93	4.37e-28	110
Msa0688490	MtrunA17_Chr2g0282051	60.465	86	31	2	10	95	17	99	2.30e-27	107
Msa0688490	MtrunA17_Chr2g0308011	88.000	50	6	0	40	89	36	85	3.21e-27	105
Msa0688490	MtrunA17_Chr4g0047761	80.702	57	10	1	39	95	70	125	1.66e-26	105
Msa0688490	MtrunA17_Chr3g0092111	66.197	71	23	1	29	99	125	194	8.86e-26	105
Msa0688490	MtrunA17_Chr7g0217581	67.143	70	22	1	29	98	221	289	1.31e-25	106
Msa0688490	MtrunA17_Chr5g0417571	59.740	77	30	1	29	105	127	202	3.63e-25	102
Msa0688490	MtrunA17_Chr6g0460731	51.042	96	45	2	4	98	59	153	3.66e-25	103
Msa0688490	MtrunA17_Chr6g0460721	48.315	89	45	1	10	98	59	146	5.48e-25	102
Msa0688490	MtrunA17_Chr6g0453321	52.174	92	43	1	7	98	103	193	4.67e-24	101
Msa0688490	MtrunA17_Chr7g0254161	60.563	71	27	1	28	98	89	158	5.97e-24	100
Msa0688490	MtrunA17_Chr4g0042481	54.321	81	33	1	19	99	104	180	7.77e-24	100
Msa0688490	MtrunA17_Chr8g0355261	60.294	68	26	1	29	96	107	173	2.60e-23	99.0
Msa0688490	MtrunA17_Chr2g0283811	67.857	56	18	0	39	94	45	100	7.34e-23	92.4
Msa0688490	MtrunA17_Chr5g0417551	59.211	76	27	1	25	100	115	186	5.02e-22	94.4
Msa0688490	MtrunA17_Chr5g0417561	59.211	76	27	1	25	100	107	178	6.54e-22	94.0
Msa0688490	MtrunA17_Chr5g0417601	58.571	70	28	1	25	94	125	193	1.00e-20	90.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0688490	AT2G28510.1	41.083	314	122	11	10	289	4	288	1.17e-54	179
Msa0688490	AT5G60200.1	39.831	236	81	7	25	255	40	219	1.52e-42	147
Msa0688490	AT3G45610.1	35.156	256	109	9	1	255	11	210	4.20e-37	132
Msa0688490	AT1G51700.1	63.158	95	31	2	21	114	16	107	1.18e-34	124
Msa0688490	AT3G55370.3	71.429	77	21	1	25	100	104	180	6.70e-33	124
Msa0688490	AT3G55370.1	71.429	77	21	1	25	100	59	135	7.68e-33	123
Msa0688490	AT3G55370.2	71.429	77	21	1	25	100	59	135	1.28e-32	123
Msa0688490	AT5G02460.1	73.611	72	18	1	25	95	78	149	4.85e-32	122
Msa0688490	AT1G21340.1	65.116	86	18	1	40	113	37	122	7.33e-32	119
Msa0688490	AT1G07640.2	65.823	79	26	1	18	95	53	131	1.61e-31	120
Msa0688490	AT5G60850.1	72.000	75	19	1	39	111	49	123	1.80e-31	119
Msa0688490	AT4G24060.1	72.727	66	18	0	30	95	42	107	2.04e-31	120
Msa0688490	AT3G52440.1	59.574	94	33	1	43	136	27	115	2.29e-31	117
Msa0688490	AT1G07640.3	65.823	79	26	1	18	95	61	139	2.30e-31	119
Msa0688490	AT3G52440.2	59.574	94	33	1	43	136	46	134	2.81e-31	117
Msa0688490	AT3G61850.2	52.066	121	52	3	27	147	49	163	2.95e-31	118
Msa0688490	AT3G61850.3	52.066	121	52	3	27	147	49	163	2.95e-31	118
Msa0688490	AT3G61850.1	52.066	121	52	3	27	147	61	175	3.38e-31	118
Msa0688490	AT3G61850.5	52.066	121	52	3	27	147	49	163	3.75e-31	118
Msa0688490	AT3G61850.4	52.066	121	52	3	27	147	61	175	4.77e-31	118
Msa0688490	AT2G37590.1	67.123	73	23	1	25	96	72	144	5.61e-31	118
Msa0688490	AT1G07640.1	69.444	72	21	1	25	95	4	75	7.52e-31	117
Msa0688490	AT2G28810.2	71.642	67	18	1	26	92	63	128	7.78e-31	118
Msa0688490	AT2G28810.1	71.642	67	18	1	26	92	80	145	1.46e-30	117
Msa0688490	AT5G65590.1	80.328	61	12	0	41	101	41	101	1.66e-30	117
Msa0688490	AT5G62940.1	77.049	61	14	0	31	91	63	123	1.96e-30	117
Msa0688490	AT1G28310.1	87.273	55	7	0	41	95	27	81	7.55e-30	115
Msa0688490	AT1G28310.2	87.273	55	7	0	41	95	51	105	1.18e-29	115
Msa0688490	AT3G21270.1	90.566	53	5	0	41	93	29	81	3.35e-29	110
Msa0688490	AT4G38000.1	72.727	66	17	1	30	94	30	95	1.07e-28	110
Msa0688490	AT4G21050.1	69.118	68	21	0	38	105	21	88	1.16e-28	109
Msa0688490	AT3G50410.1	72.308	65	18	0	33	97	22	86	2.24e-28	109
Msa0688490	AT5G66940.1	58.511	94	29	3	37	123	28	118	5.64e-28	108
Msa0688490	AT4G00940.1	80.702	57	11	0	39	95	66	122	5.84e-28	109
Msa0688490	AT4G00940.3	80.702	57	11	0	39	95	66	122	5.84e-28	109
Msa0688490	AT4G00940.2	80.702	57	11	0	39	95	66	122	5.84e-28	109
Msa0688490	AT1G64620.1	72.131	61	17	0	30	90	38	98	2.66e-27	108
Msa0688490	AT2G46590.1	63.158	76	24	1	13	88	44	115	3.73e-27	108
Msa0688490	AT2G46590.2	63.158	76	24	1	13	88	56	127	4.66e-27	108
Msa0688490	AT1G47655.1	75.439	57	14	0	33	89	21	77	4.48e-26	102
Msa0688490	AT5G62430.1	47.959	98	45	2	3	100	22	113	1.78e-25	103
Msa0688490	AT3G47500.1	52.632	95	41	1	4	98	77	167	1.87e-25	105
Msa0688490	AT5G39660.3	61.333	75	25	1	24	98	125	195	2.47e-24	102
Msa0688490	AT5G39660.1	61.333	75	25	1	24	98	125	195	2.47e-24	102
Msa0688490	AT5G39660.2	61.333	75	25	1	24	98	125	195	2.47e-24	102
Msa0688490	AT4G21080.1	56.579	76	24	1	43	109	27	102	6.07e-24	97.8
Msa0688490	AT1G69570.1	53.488	86	36	1	9	94	104	185	6.44e-24	100
Msa0688490	AT4G21040.1	75.000	52	13	0	43	94	27	78	2.19e-23	95.9
Msa0688490	AT1G26790.1	55.263	76	33	1	20	94	89	164	2.86e-23	98.2
Msa0688490	AT2G34140.1	60.000	65	22	1	30	94	51	111	1.61e-22	92.0
Msa0688490	AT1G29160.1	66.071	56	19	0	39	94	60	115	2.76e-22	91.7
Msa0688490	AT4G21030.1	51.471	68	33	0	38	105	18	85	1.19e-15	74.3

Find 101 sgRNAs with CRISPR-Local

Find 304 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGGTTTGGTTTCCTAAAA+TGG	0.139828	4_4:+54545791	None:intergenic
GGGAAACAAGTGAGTGGATT+TGG	0.231178	4_4:+54545903	None:intergenic
TTAGGGAACAAAATGAAAAT+AGG	0.240393	4_4:-54545527	Msa0688490:CDS
TGGATTCTTCGAAGCACTTA+TGG	0.247834	4_4:-54545715	Msa0688490:CDS
GCTTCTGGTTGAGGAGGTTT+TGG	0.248273	4_4:+54546143	None:intergenic
ATTGCCTTGTCGCACTATTA+AGG	0.253233	4_4:+54545324	None:intergenic
GCAAGTCTTGTAGGAGATAT+TGG	0.255429	4_4:-54546037	Msa0688490:CDS
TCATTACTCTTTCTCTCTCT+TGG	0.271913	4_4:-54547185	Msa0688490:five_prime_UTR
GAATGGTCTCACTTCATCTT+GGG	0.274434	4_4:-54545424	Msa0688490:CDS
GATATTTCTGCAAGTCTTGT+AGG	0.294030	4_4:-54546046	Msa0688490:CDS
ACACCATACATTAGTTTGAT+AGG	0.316212	4_4:-54545191	Msa0688490:three_prime_UTR
GGAATGGTCTCACTTCATCT+TGG	0.324184	4_4:-54545425	Msa0688490:CDS
ATTGTTGTAGTAACAAAATT+TGG	0.325870	4_4:+54546088	None:intergenic
TTTCCATGGCAGTTCAATAA+TGG	0.330980	4_4:-54545492	Msa0688490:CDS
TGCGGCTGCAATTACGGTTA+TGG	0.348385	4_4:-54545234	Msa0688490:three_prime_UTR
GGAAACAAGTGAGTGGATTT+GGG	0.349121	4_4:+54545904	None:intergenic
CATTACTCTTTCTCTCTCTT+GGG	0.353198	4_4:-54547184	Msa0688490:five_prime_UTR
AACAAGAAATGTGTAATGTT+AGG	0.357073	4_4:-54545545	Msa0688490:CDS
ACGAGACGGTGTCTACATTA+GGG	0.358019	4_4:+54545380	None:intergenic
ATGGCTGAAATGGATCATTT+AGG	0.361202	4_4:-54545459	Msa0688490:CDS
TTATATTGCGGCTGCAATTA+CGG	0.361215	4_4:-54545240	Msa0688490:three_prime_UTR
TGGACTGCAATTTAGAACTA+TGG	0.362313	4_4:-54545214	Msa0688490:three_prime_UTR
TTGATTAAGAAGTGTTTGTA+TGG	0.363457	4_4:-54546968	Msa0688490:exon
AGACACAAATATGGCTGAAA+TGG	0.367887	4_4:-54545469	Msa0688490:CDS
CACGAGACGGTGTCTACATT+AGG	0.377042	4_4:+54545379	None:intergenic
CAATAATGGAGACACAAATA+TGG	0.378026	4_4:-54545478	Msa0688490:CDS
GGATTCTTCGAAGCACTTAT+GGG	0.384728	4_4:-54545714	Msa0688490:CDS
CAAGACTTGCAGAAATATCT+TGG	0.387546	4_4:+54546050	None:intergenic
TAGGGAACAAAATGAAAATA+GGG	0.390383	4_4:-54545526	Msa0688490:CDS
AGGTAAGTAATGAAAATCTA+AGG	0.411275	4_4:+54547134	None:intergenic
AGGAATGTTCCTGTTGGTGG+GGG	0.412807	4_4:-54545996	Msa0688490:CDS
TTCTTCTAGTGGACAACAAC+AGG	0.413714	4_4:-54546938	Msa0688490:intron
TGTGAGATCATTGGAATCAT+AGG	0.425382	4_4:+54545872	None:intergenic
GGATTTGGGTTGGTTTGAAA+TGG	0.429582	4_4:+54545918	None:intergenic
GTTGATAATGGCAATGCTAA+TGG	0.432043	4_4:-54545651	Msa0688490:CDS
TACCACATGAGGTGTTGGTT+TGG	0.441239	4_4:+54545778	None:intergenic
AGCATTAGCTAGGCTTCAAA+AGG	0.442460	4_4:-54545847	Msa0688490:CDS
GCTAACCAAGTCAATGAAGT+TGG	0.443836	4_4:-54544988	Msa0688490:three_prime_UTR
GTTTGAGGAGAGAAAAGAAA+AGG	0.447592	4_4:+54547066	None:intergenic
ACAAGAAATGTGTAATGTTA+GGG	0.450520	4_4:-54545544	Msa0688490:CDS
TTCCCTATCAAACTAATGTA+TGG	0.459493	4_4:+54545188	None:intergenic
GAAGAATCCATGGTTGTTTG+AGG	0.461029	4_4:+54545728	None:intergenic
ATGCTCATTTCATGATCATA+AGG	0.463078	4_4:+54545603	None:intergenic
GCAAATGTCTAACCAGTCAT+TGG	0.467897	4_4:-54546207	Msa0688490:CDS
TGTGGTATTCTTAACAATCA+TGG	0.480447	4_4:-54545762	Msa0688490:CDS
CTCTTAAAATCTTTATATTG+CGG	0.480943	4_4:-54545252	Msa0688490:three_prime_UTR
AAGAAGTGTTTGTATGGGGA+TGG	0.488483	4_4:-54546962	Msa0688490:exon
CACCATACATTAGTTTGATA+GGG	0.488501	4_4:-54545190	Msa0688490:three_prime_UTR
ATCCATGGTTGTTTGAGGAA+TGG	0.489166	4_4:+54545733	None:intergenic
AGGCTTCAAAAGGGTCAACT+TGG	0.489311	4_4:-54545837	Msa0688490:CDS
ACAAGTGAGTGGATTTGGGT+TGG	0.491105	4_4:+54545908	None:intergenic
TAAGGAATGTTCCTGTTGGT+GGG	0.496140	4_4:-54545998	Msa0688490:CDS
AATGGTCTCACTTCATCTTG+GGG	0.497543	4_4:-54545423	Msa0688490:CDS
TGTAGGAGATATTGGACTAA+AGG	0.499003	4_4:-54546029	Msa0688490:CDS
GTGTTGTTTGAGTCACATCT+TGG	0.500769	4_4:+54546110	None:intergenic
TGAATAAGGAAATGAAAGAA+AGG	0.501058	4_4:+54547040	None:intergenic
ATGGATCATTTAGGAAGAGC+TGG	0.502060	4_4:-54545450	Msa0688490:CDS
TAATGCTAGTGTGAGATCAT+TGG	0.503293	4_4:+54545863	None:intergenic
TTGGAATCATAGGACATTGA+AGG	0.508739	4_4:+54545882	None:intergenic
TTAGGAAGAGCTGGTTGGAA+TGG	0.509850	4_4:-54545441	Msa0688490:CDS
CTAAGGAATGTTCCTGTTGG+TGG	0.517333	4_4:-54545999	Msa0688490:CDS
TGATTAAGAAGTGTTTGTAT+GGG	0.520497	4_4:-54546967	Msa0688490:exon
AAGAATACCACATGAGGTGT+TGG	0.521494	4_4:+54545773	None:intergenic
TCTCTCCAACTTCATTGACT+TGG	0.524005	4_4:+54544983	None:intergenic
ATGAAAGAAAGGAAAGTTTG+AGG	0.525614	4_4:+54547051	None:intergenic
GACTAATGCAGTTGCAATTG+CGG	0.539445	4_4:-54545296	Msa0688490:three_prime_UTR
AGAAAAGAAAAGGATAGAGA+TGG	0.543014	4_4:+54547076	None:intergenic
TGCAGAATCTTTACTACATG+GGG	0.546230	4_4:-54545677	Msa0688490:CDS
ATGGGGATGGATTCTTCTAG+TGG	0.553754	4_4:-54546949	Msa0688490:CDS
TTTAGAGCTTCTGGTTGAGG+AGG	0.557206	4_4:+54546137	None:intergenic
AAGGAATGTTCCTGTTGGTG+GGG	0.558824	4_4:-54545997	Msa0688490:CDS
TTAACATGCTCTCCAATGAC+TGG	0.564124	4_4:+54546195	None:intergenic
GGACTAAAGGTGGTACACTA+AGG	0.568077	4_4:-54546016	Msa0688490:CDS
AATGCTAATGGCAATGGTAA+TGG	0.568080	4_4:-54545639	Msa0688490:CDS
ATCATTTAGGAAGAGCTGGT+TGG	0.573027	4_4:-54545446	Msa0688490:CDS
ACCAATTAAAAGACACGAGT+AGG	0.575185	4_4:+54545045	None:intergenic
CGAGACGGTGTCTACATTAG+GGG	0.579103	4_4:+54545381	None:intergenic
TGTGCAGAATCTTTACTACA+TGG	0.579242	4_4:-54545679	Msa0688490:CDS
TGGAATCATAGGACATTGAA+GGG	0.583945	4_4:+54545883	None:intergenic
GGAATGTTCCTGTTGGTGGG+GGG	0.588003	4_4:-54545995	Msa0688490:CDS
ATCTCACACTAGCATTAGCT+AGG	0.588061	4_4:-54545857	Msa0688490:CDS
GTGCAGAATCTTTACTACAT+GGG	0.589427	4_4:-54545678	Msa0688490:CDS
CATCATCAGCTTCATCAAAG+AGG	0.595811	4_4:-54545953	Msa0688490:CDS
AACCAAACCAACACCTCATG+TGG	0.607094	4_4:-54545780	Msa0688490:CDS
AACCATTCCTCAAACAACCA+TGG	0.607175	4_4:-54545735	Msa0688490:CDS
TAAGTGCTTCGAAGAATCCA+TGG	0.607290	4_4:+54545718	None:intergenic
ATGGTCTCACTTCATCTTGG+GGG	0.616298	4_4:-54545422	Msa0688490:CDS
AATGGCAATGCTAATGGCAA+TGG	0.626445	4_4:-54545645	Msa0688490:CDS
TCTCCATTATTGAACTGCCA+TGG	0.629912	4_4:+54545489	None:intergenic
GCATTAGCTAGGCTTCAAAA+GGG	0.630153	4_4:-54545846	Msa0688490:CDS
GTCGTACAAGATGATGGATG+AGG	0.632055	4_4:+54547223	None:intergenic
GAGACGGTGTCTACATTAGG+GGG	0.640287	4_4:+54545382	None:intergenic
ATTGTTAAGAATACCACATG+AGG	0.643870	4_4:+54545767	None:intergenic
AGGAGATATTGGACTAAAGG+TGG	0.646558	4_4:-54546026	Msa0688490:CDS
GATTAAGAAGTGTTTGTATG+GGG	0.649738	4_4:-54546966	Msa0688490:exon
ACACTAAGGAATGTTCCTGT+TGG	0.660187	4_4:-54546002	Msa0688490:CDS
ATTGAAGGGAAACAAGTGAG+TGG	0.662440	4_4:+54545897	None:intergenic
TGATGATGCAAAGTGAAGTG+AGG	0.684560	4_4:+54547114	None:intergenic
TGAACCTTAATAGTGCGACA+AGG	0.723900	4_4:-54545328	Msa0688490:three_prime_UTR
TGATATTTAGAATCACGAGA+CGG	0.729031	4_4:+54545366	None:intergenic
GCAGAATCTTTACTACATGG+GGG	0.765910	4_4:-54545676	Msa0688490:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACTAATAAAAATAAAATAG+AGG	+	chr4_4:54547097-54547116	None:intergenic	10.0%
!!	AACTAATAAAAATAAAATAG+AGG	+	chr4_4:54547097-54547116	None:intergenic	10.0%
!!!	TTTTTGTGTATATATGAATA+AGG	+	chr4_4:54545204-54545223	None:intergenic	15.0%
!!	AAAAAAATCAGGAAAATAAT+AGG	+	chr4_4:54545455-54545474	None:intergenic	15.0%
!!	TAACATTATGTTTGAATTAA+CGG	-	chr4_4:54545783-54545802	Msa0688490:CDS	15.0%
!!	TCACAAAAATTTGTAATATT+TGG	-	chr4_4:54545958-54545977	Msa0688490:CDS	15.0%
!!!	TTTTTGTGTATATATGAATA+AGG	+	chr4_4:54545204-54545223	None:intergenic	15.0%
!!	AAAAAAATCAGGAAAATAAT+AGG	+	chr4_4:54545455-54545474	None:intergenic	15.0%
!!	TAACATTATGTTTGAATTAA+CGG	-	chr4_4:54545783-54545802	Msa0688490:CDS	15.0%
!!	TCACAAAAATTTGTAATATT+TGG	-	chr4_4:54545958-54545977	Msa0688490:CDS	15.0%
!!	CAAAGAAGAAAAAAAAAATC+AGG	+	chr4_4:54545466-54545485	None:intergenic	20.0%
!!	TTCCATCAAATATTAATTCT+AGG	+	chr4_4:54545613-54545632	None:intergenic	20.0%
!!!	TTTACTTTATTTGATCTAGA+GGG	+	chr4_4:54545690-54545709	None:intergenic	20.0%
!!!	TTTTACTTTATTTGATCTAG+AGG	+	chr4_4:54545691-54545710	None:intergenic	20.0%
!!	ATTGTTGTAGTAACAAAATT+TGG	+	chr4_4:54546142-54546161	None:intergenic	20.0%
!!	CTCTTAAAATCTTTATATTG+CGG	-	chr4_4:54546975-54546994	Msa0688490:five_prime_UTR	20.0%
!!!	CACAAAATTTTGACAATTTA+AGG	+	chr4_4:54547162-54547181	None:intergenic	20.0%
!!	CAAAGAAGAAAAAAAAAATC+AGG	+	chr4_4:54545466-54545485	None:intergenic	20.0%
!!	TTCCATCAAATATTAATTCT+AGG	+	chr4_4:54545613-54545632	None:intergenic	20.0%
!!!	TTTACTTTATTTGATCTAGA+GGG	+	chr4_4:54545690-54545709	None:intergenic	20.0%
!!!	TTTTACTTTATTTGATCTAG+AGG	+	chr4_4:54545691-54545710	None:intergenic	20.0%
!!	ATTGTTGTAGTAACAAAATT+TGG	+	chr4_4:54546142-54546161	None:intergenic	20.0%
!!	CTCTTAAAATCTTTATATTG+CGG	-	chr4_4:54546975-54546994	Msa0688490:five_prime_UTR	20.0%
!!!	CACAAAATTTTGACAATTTA+AGG	+	chr4_4:54547162-54547181	None:intergenic	20.0%
!	AGGTAAGTAATGAAAATCTA+AGG	+	chr4_4:54545096-54545115	None:intergenic	25.0%
!	TGAATAAGGAAATGAAAGAA+AGG	+	chr4_4:54545190-54545209	None:intergenic	25.0%
!!!	CTAGAGATAAAAAAGTGTTA+TGG	+	chr4_4:54545227-54545246	None:intergenic	25.0%
!!!	TTGATTAAGAAGTGTTTGTA+TGG	-	chr4_4:54545259-54545278	Msa0688490:three_prime_UTR	25.0%
!!!	TGATTAAGAAGTGTTTGTAT+GGG	-	chr4_4:54545260-54545279	Msa0688490:three_prime_UTR	25.0%
!	GTAGTACTAAAAATTCATGA+AGG	+	chr4_4:54545412-54545431	None:intergenic	25.0%
!!!	CATGAATTTTTAGTACTACT+TGG	-	chr4_4:54545413-54545432	Msa0688490:CDS	25.0%
!!!	ACTAATTTTATTTCACCCAA+GGG	+	chr4_4:54545496-54545515	None:intergenic	25.0%
!!!	TACTAATTTTATTTCACCCA+AGG	+	chr4_4:54545497-54545516	None:intergenic	25.0%
!	ATATTTGATGGAATAAAGTC+AGG	-	chr4_4:54545620-54545639	Msa0688490:CDS	25.0%
!	TATTTGATGGAATAAAGTCA+GGG	-	chr4_4:54545621-54545640	Msa0688490:CDS	25.0%
!!	TTACTTTATTTGATCTAGAG+GGG	+	chr4_4:54545689-54545708	None:intergenic	25.0%
!!	TTATTAATGGATGTACATGT+TGG	-	chr4_4:54545726-54545745	Msa0688490:CDS	25.0%
!!	TATTAATGGATGTACATGTT+GGG	-	chr4_4:54545727-54545746	Msa0688490:CDS	25.0%
!	ACATATGTATGTACTATGTA+TGG	-	chr4_4:54545930-54545949	Msa0688490:CDS	25.0%
!!	GAACATGATTTTTCCATTTT+AGG	-	chr4_4:54546423-54546442	Msa0688490:intron	25.0%
!	AACAAGAAATGTGTAATGTT+AGG	-	chr4_4:54546682-54546701	Msa0688490:intron	25.0%
!	ACAAGAAATGTGTAATGTTA+GGG	-	chr4_4:54546683-54546702	Msa0688490:intron	25.0%
!	TTAGGGAACAAAATGAAAAT+AGG	-	chr4_4:54546700-54546719	Msa0688490:intron	25.0%
!	TAGGGAACAAAATGAAAATA+GGG	-	chr4_4:54546701-54546720	Msa0688490:intron	25.0%
!!!	AATGAAAATAGGGTTTTGTT+AGG	-	chr4_4:54546711-54546730	Msa0688490:intron	25.0%
!!!	ATTTTAAGAGGTCTTTAGAA+TGG	+	chr4_4:54546965-54546984	None:intergenic	25.0%
!!	CGCAATATAAAGATTTTAAG+AGG	+	chr4_4:54546977-54546996	None:intergenic	25.0%
!	AGGTAAGTAATGAAAATCTA+AGG	+	chr4_4:54545096-54545115	None:intergenic	25.0%
!	TGAATAAGGAAATGAAAGAA+AGG	+	chr4_4:54545190-54545209	None:intergenic	25.0%
!!!	CTAGAGATAAAAAAGTGTTA+TGG	+	chr4_4:54545227-54545246	None:intergenic	25.0%
!!!	TTGATTAAGAAGTGTTTGTA+TGG	-	chr4_4:54545259-54545278	Msa0688490:three_prime_UTR	25.0%
!!!	TGATTAAGAAGTGTTTGTAT+GGG	-	chr4_4:54545260-54545279	Msa0688490:three_prime_UTR	25.0%
!	GTAGTACTAAAAATTCATGA+AGG	+	chr4_4:54545412-54545431	None:intergenic	25.0%
!!!	CATGAATTTTTAGTACTACT+TGG	-	chr4_4:54545413-54545432	Msa0688490:CDS	25.0%
!!!	ACTAATTTTATTTCACCCAA+GGG	+	chr4_4:54545496-54545515	None:intergenic	25.0%
!!!	TACTAATTTTATTTCACCCA+AGG	+	chr4_4:54545497-54545516	None:intergenic	25.0%
!	ATATTTGATGGAATAAAGTC+AGG	-	chr4_4:54545620-54545639	Msa0688490:CDS	25.0%
!	TATTTGATGGAATAAAGTCA+GGG	-	chr4_4:54545621-54545640	Msa0688490:CDS	25.0%
!!	TTACTTTATTTGATCTAGAG+GGG	+	chr4_4:54545689-54545708	None:intergenic	25.0%
!!	TTATTAATGGATGTACATGT+TGG	-	chr4_4:54545726-54545745	Msa0688490:CDS	25.0%
!!	TATTAATGGATGTACATGTT+GGG	-	chr4_4:54545727-54545746	Msa0688490:CDS	25.0%
!	ACATATGTATGTACTATGTA+TGG	-	chr4_4:54545930-54545949	Msa0688490:CDS	25.0%
!!	GAACATGATTTTTCCATTTT+AGG	-	chr4_4:54546423-54546442	Msa0688490:intron	25.0%
!	AACAAGAAATGTGTAATGTT+AGG	-	chr4_4:54546682-54546701	Msa0688490:intron	25.0%
!	ACAAGAAATGTGTAATGTTA+GGG	-	chr4_4:54546683-54546702	Msa0688490:intron	25.0%
!	TTAGGGAACAAAATGAAAAT+AGG	-	chr4_4:54546700-54546719	Msa0688490:intron	25.0%
!	TAGGGAACAAAATGAAAATA+GGG	-	chr4_4:54546701-54546720	Msa0688490:intron	25.0%
!!!	AATGAAAATAGGGTTTTGTT+AGG	-	chr4_4:54546711-54546730	Msa0688490:intron	25.0%
!!!	ATTTTAAGAGGTCTTTAGAA+TGG	+	chr4_4:54546965-54546984	None:intergenic	25.0%
!!	CGCAATATAAAGATTTTAAG+AGG	+	chr4_4:54546977-54546996	None:intergenic	25.0%
!	AATTTTGTCGTACAAGATGA+TGG	+	chr4_4:54545013-54545032	None:intergenic	30.0%
	AGAAAAGAAAAGGATAGAGA+TGG	+	chr4_4:54545154-54545173	None:intergenic	30.0%
	ATGAAAGAAAGGAAAGTTTG+AGG	+	chr4_4:54545179-54545198	None:intergenic	30.0%
!!	GATTAAGAAGTGTTTGTATG+GGG	-	chr4_4:54545261-54545280	Msa0688490:three_prime_UTR	30.0%
	AAATTCATGAAGGACAAAGA+TGG	+	chr4_4:54545402-54545421	None:intergenic	30.0%
	GGCCTAGAATTAATATTTGA+TGG	-	chr4_4:54545608-54545627	Msa0688490:CDS	30.0%
	GAATCAGAAAAGTTACATTG+TGG	-	chr4_4:54545817-54545836	Msa0688490:CDS	30.0%
	ACACAACACAACAAATTTAG+TGG	+	chr4_4:54545993-54546012	None:intergenic	30.0%
	ATTGTTAAGAATACCACATG+AGG	+	chr4_4:54546463-54546482	None:intergenic	30.0%
	TGTGGTATTCTTAACAATCA+TGG	-	chr4_4:54546465-54546484	Msa0688490:intron	30.0%
	ATGCTCATTTCATGATCATA+AGG	+	chr4_4:54546627-54546646	None:intergenic	30.0%
!	CAATAATGGAGACACAAATA+TGG	-	chr4_4:54546749-54546768	Msa0688490:intron	30.0%
!	TGATATTTAGAATCACGAGA+CGG	+	chr4_4:54546864-54546883	None:intergenic	30.0%
!	ACACCATACATTAGTTTGAT+AGG	-	chr4_4:54547036-54547055	Msa0688490:five_prime_UTR	30.0%
!	CACCATACATTAGTTTGATA+GGG	-	chr4_4:54547037-54547056	Msa0688490:five_prime_UTR	30.0%
	TTCCCTATCAAACTAATGTA+TGG	+	chr4_4:54547042-54547061	None:intergenic	30.0%
!	AATTTTGTCGTACAAGATGA+TGG	+	chr4_4:54545013-54545032	None:intergenic	30.0%
	AGAAAAGAAAAGGATAGAGA+TGG	+	chr4_4:54545154-54545173	None:intergenic	30.0%
	ATGAAAGAAAGGAAAGTTTG+AGG	+	chr4_4:54545179-54545198	None:intergenic	30.0%
!!	GATTAAGAAGTGTTTGTATG+GGG	-	chr4_4:54545261-54545280	Msa0688490:three_prime_UTR	30.0%
	AAATTCATGAAGGACAAAGA+TGG	+	chr4_4:54545402-54545421	None:intergenic	30.0%
	GGCCTAGAATTAATATTTGA+TGG	-	chr4_4:54545608-54545627	Msa0688490:CDS	30.0%
	GAATCAGAAAAGTTACATTG+TGG	-	chr4_4:54545817-54545836	Msa0688490:CDS	30.0%
	ACACAACACAACAAATTTAG+TGG	+	chr4_4:54545993-54546012	None:intergenic	30.0%
	ATTGTTAAGAATACCACATG+AGG	+	chr4_4:54546463-54546482	None:intergenic	30.0%
	TGTGGTATTCTTAACAATCA+TGG	-	chr4_4:54546465-54546484	Msa0688490:intron	30.0%
	ATGCTCATTTCATGATCATA+AGG	+	chr4_4:54546627-54546646	None:intergenic	30.0%
!	CAATAATGGAGACACAAATA+TGG	-	chr4_4:54546749-54546768	Msa0688490:intron	30.0%
!	TGATATTTAGAATCACGAGA+CGG	+	chr4_4:54546864-54546883	None:intergenic	30.0%
!	ACACCATACATTAGTTTGAT+AGG	-	chr4_4:54547036-54547055	Msa0688490:five_prime_UTR	30.0%
!	CACCATACATTAGTTTGATA+GGG	-	chr4_4:54547037-54547056	Msa0688490:five_prime_UTR	30.0%
	TTCCCTATCAAACTAATGTA+TGG	+	chr4_4:54547042-54547061	None:intergenic	30.0%
	TCATTACTCTTTCTCTCTCT+TGG	-	chr4_4:54545042-54545061	Msa0688490:three_prime_UTR	35.0%
	CATTACTCTTTCTCTCTCTT+GGG	-	chr4_4:54545043-54545062	Msa0688490:three_prime_UTR	35.0%
	GTTTGAGGAGAGAAAAGAAA+AGG	+	chr4_4:54545164-54545183	None:intergenic	35.0%
	ACTACTTGGAAGAATGAACA+AGG	-	chr4_4:54545427-54545446	Msa0688490:CDS	35.0%
	TTAGCATCTTCATCTTTGTG+AGG	-	chr4_4:54545663-54545682	Msa0688490:CDS	35.0%
	CAAGACTTGCAGAAATATCT+TGG	+	chr4_4:54546180-54546199	None:intergenic	35.0%
!	GATATTTCTGCAAGTCTTGT+AGG	-	chr4_4:54546181-54546200	Msa0688490:CDS	35.0%
	TGTAGGAGATATTGGACTAA+AGG	-	chr4_4:54546198-54546217	Msa0688490:CDS	35.0%
	TGGAATCATAGGACATTGAA+GGG	+	chr4_4:54546347-54546366	None:intergenic	35.0%
	TTGGAATCATAGGACATTGA+AGG	+	chr4_4:54546348-54546367	None:intergenic	35.0%
	TGTGAGATCATTGGAATCAT+AGG	+	chr4_4:54546358-54546377	None:intergenic	35.0%
	TAATGCTAGTGTGAGATCAT+TGG	+	chr4_4:54546367-54546386	None:intergenic	35.0%
!!	GTTGGTTTGGTTTCCTAAAA+TGG	+	chr4_4:54546439-54546458	None:intergenic	35.0%
	TGTGCAGAATCTTTACTACA+TGG	-	chr4_4:54546548-54546567	Msa0688490:intron	35.0%
	GTGCAGAATCTTTACTACAT+GGG	-	chr4_4:54546549-54546568	Msa0688490:intron	35.0%
	TGCAGAATCTTTACTACATG+GGG	-	chr4_4:54546550-54546569	Msa0688490:intron	35.0%
!!	GTTGATAATGGCAATGCTAA+TGG	-	chr4_4:54546576-54546595	Msa0688490:intron	35.0%
	AATGCTAATGGCAATGGTAA+TGG	-	chr4_4:54546588-54546607	Msa0688490:intron	35.0%
	TTTCCATGGCAGTTCAATAA+TGG	-	chr4_4:54546735-54546754	Msa0688490:intron	35.0%
	AGACACAAATATGGCTGAAA+TGG	-	chr4_4:54546758-54546777	Msa0688490:intron	35.0%
	ATGGCTGAAATGGATCATTT+AGG	-	chr4_4:54546768-54546787	Msa0688490:intron	35.0%
	TTATATTGCGGCTGCAATTA+CGG	-	chr4_4:54546987-54547006	Msa0688490:five_prime_UTR	35.0%
!	TGGACTGCAATTTAGAACTA+TGG	-	chr4_4:54547013-54547032	Msa0688490:five_prime_UTR	35.0%
	ACCAATTAAAAGACACGAGT+AGG	+	chr4_4:54547185-54547204	None:intergenic	35.0%
	TCATTACTCTTTCTCTCTCT+TGG	-	chr4_4:54545042-54545061	Msa0688490:three_prime_UTR	35.0%
	CATTACTCTTTCTCTCTCTT+GGG	-	chr4_4:54545043-54545062	Msa0688490:three_prime_UTR	35.0%
	GTTTGAGGAGAGAAAAGAAA+AGG	+	chr4_4:54545164-54545183	None:intergenic	35.0%
	ACTACTTGGAAGAATGAACA+AGG	-	chr4_4:54545427-54545446	Msa0688490:CDS	35.0%
	TTAGCATCTTCATCTTTGTG+AGG	-	chr4_4:54545663-54545682	Msa0688490:CDS	35.0%
	CAAGACTTGCAGAAATATCT+TGG	+	chr4_4:54546180-54546199	None:intergenic	35.0%
!	GATATTTCTGCAAGTCTTGT+AGG	-	chr4_4:54546181-54546200	Msa0688490:CDS	35.0%
	TGTAGGAGATATTGGACTAA+AGG	-	chr4_4:54546198-54546217	Msa0688490:CDS	35.0%
	TGGAATCATAGGACATTGAA+GGG	+	chr4_4:54546347-54546366	None:intergenic	35.0%
	TTGGAATCATAGGACATTGA+AGG	+	chr4_4:54546348-54546367	None:intergenic	35.0%
	TGTGAGATCATTGGAATCAT+AGG	+	chr4_4:54546358-54546377	None:intergenic	35.0%
	TAATGCTAGTGTGAGATCAT+TGG	+	chr4_4:54546367-54546386	None:intergenic	35.0%
!!	GTTGGTTTGGTTTCCTAAAA+TGG	+	chr4_4:54546439-54546458	None:intergenic	35.0%
	TGTGCAGAATCTTTACTACA+TGG	-	chr4_4:54546548-54546567	Msa0688490:intron	35.0%
	GTGCAGAATCTTTACTACAT+GGG	-	chr4_4:54546549-54546568	Msa0688490:intron	35.0%
	TGCAGAATCTTTACTACATG+GGG	-	chr4_4:54546550-54546569	Msa0688490:intron	35.0%
!!	GTTGATAATGGCAATGCTAA+TGG	-	chr4_4:54546576-54546595	Msa0688490:intron	35.0%
	AATGCTAATGGCAATGGTAA+TGG	-	chr4_4:54546588-54546607	Msa0688490:intron	35.0%
	TTTCCATGGCAGTTCAATAA+TGG	-	chr4_4:54546735-54546754	Msa0688490:intron	35.0%
	AGACACAAATATGGCTGAAA+TGG	-	chr4_4:54546758-54546777	Msa0688490:intron	35.0%
	ATGGCTGAAATGGATCATTT+AGG	-	chr4_4:54546768-54546787	Msa0688490:intron	35.0%
	TTATATTGCGGCTGCAATTA+CGG	-	chr4_4:54546987-54547006	Msa0688490:five_prime_UTR	35.0%
!	TGGACTGCAATTTAGAACTA+TGG	-	chr4_4:54547013-54547032	Msa0688490:five_prime_UTR	35.0%
	ACCAATTAAAAGACACGAGT+AGG	+	chr4_4:54547185-54547204	None:intergenic	35.0%
!	TGATGATGCAAAGTGAAGTG+AGG	+	chr4_4:54545116-54545135	None:intergenic	40.0%
!!	AAGAAGTGTTTGTATGGGGA+TGG	-	chr4_4:54545265-54545284	Msa0688490:three_prime_UTR	40.0%
!	TTCTTCTAGTGGACAACAAC+AGG	-	chr4_4:54545289-54545308	Msa0688490:three_prime_UTR	40.0%
	TCATGAAGGACAAAGATGGA+AGG	+	chr4_4:54545398-54545417	None:intergenic	40.0%
!	TCTTCTTTGAAGAACACCCT+TGG	-	chr4_4:54545477-54545496	Msa0688490:CDS	40.0%
!	CTTCTTTGAAGAACACCCTT+GGG	-	chr4_4:54545478-54545497	Msa0688490:CDS	40.0%
	GCTGTATACATGTGGTTCAT+AGG	+	chr4_4:54545585-54545604	None:intergenic	40.0%
	ATGAACCACATGTATACAGC+TGG	-	chr4_4:54545585-54545604	Msa0688490:CDS	40.0%
	TGAACCACATGTATACAGCT+GGG	-	chr4_4:54545586-54545605	Msa0688490:CDS	40.0%
	TTGATGGAATAAAGTCAGGG+AGG	-	chr4_4:54545624-54545643	Msa0688490:CDS	40.0%
	GCAAATGTCTAACCAGTCAT+TGG	-	chr4_4:54546020-54546039	Msa0688490:CDS	40.0%
	TTAACATGCTCTCCAATGAC+TGG	+	chr4_4:54546035-54546054	None:intergenic	40.0%
!!!	CATTTTAGAGCTTCTGGTTG+AGG	+	chr4_4:54546096-54546115	None:intergenic	40.0%
!!!	CTTGGACATTTTAGAGCTTC+TGG	+	chr4_4:54546102-54546121	None:intergenic	40.0%
!!	GTGTTGTTTGAGTCACATCT+TGG	+	chr4_4:54546120-54546139	None:intergenic	40.0%
!	GCAAGTCTTGTAGGAGATAT+TGG	-	chr4_4:54546190-54546209	Msa0688490:CDS	40.0%
	AGGAGATATTGGACTAAAGG+TGG	-	chr4_4:54546201-54546220	Msa0688490:CDS	40.0%
	ACACTAAGGAATGTTCCTGT+TGG	-	chr4_4:54546225-54546244	Msa0688490:intron	40.0%
	TAAGGAATGTTCCTGTTGGT+GGG	-	chr4_4:54546229-54546248	Msa0688490:intron	40.0%
	CATCATCAGCTTCATCAAAG+AGG	-	chr4_4:54546274-54546293	Msa0688490:intron	40.0%
!!	GGATTTGGGTTGGTTTGAAA+TGG	+	chr4_4:54546312-54546331	None:intergenic	40.0%
!	GGAAACAAGTGAGTGGATTT+GGG	+	chr4_4:54546326-54546345	None:intergenic	40.0%
	ATTGAAGGGAAACAAGTGAG+TGG	+	chr4_4:54546333-54546352	None:intergenic	40.0%
	ATCTCACACTAGCATTAGCT+AGG	-	chr4_4:54546370-54546389	Msa0688490:intron	40.0%
	AGCATTAGCTAGGCTTCAAA+AGG	-	chr4_4:54546380-54546399	Msa0688490:intron	40.0%
	GCATTAGCTAGGCTTCAAAA+GGG	-	chr4_4:54546381-54546400	Msa0688490:intron	40.0%
	AAGAATACCACATGAGGTGT+TGG	+	chr4_4:54546457-54546476	None:intergenic	40.0%
	AACCATTCCTCAAACAACCA+TGG	-	chr4_4:54546492-54546511	Msa0688490:intron	40.0%
	ATCCATGGTTGTTTGAGGAA+TGG	+	chr4_4:54546497-54546516	None:intergenic	40.0%
	GAAGAATCCATGGTTGTTTG+AGG	+	chr4_4:54546502-54546521	None:intergenic	40.0%
	TAAGTGCTTCGAAGAATCCA+TGG	+	chr4_4:54546512-54546531	None:intergenic	40.0%
!	TGGATTCTTCGAAGCACTTA+TGG	-	chr4_4:54546512-54546531	Msa0688490:intron	40.0%
!	GGATTCTTCGAAGCACTTAT+GGG	-	chr4_4:54546513-54546532	Msa0688490:intron	40.0%
	GCAGAATCTTTACTACATGG+GGG	-	chr4_4:54546551-54546570	Msa0688490:intron	40.0%
	AATGGCAATGCTAATGGCAA+TGG	-	chr4_4:54546582-54546601	Msa0688490:intron	40.0%
!!!	GGGTTTTGTTAGGTTTTCCA+TGG	-	chr4_4:54546721-54546740	Msa0688490:intron	40.0%
	TCTCCATTATTGAACTGCCA+TGG	+	chr4_4:54546741-54546760	None:intergenic	40.0%
	ATGGATCATTTAGGAAGAGC+TGG	-	chr4_4:54546777-54546796	Msa0688490:intron	40.0%
	ATCATTTAGGAAGAGCTGGT+TGG	-	chr4_4:54546781-54546800	Msa0688490:intron	40.0%
	GAATGGTCTCACTTCATCTT+GGG	-	chr4_4:54546803-54546822	Msa0688490:intron	40.0%
	AATGGTCTCACTTCATCTTG+GGG	-	chr4_4:54546804-54546823	Msa0688490:intron	40.0%
	TGAACCTTAATAGTGCGACA+AGG	-	chr4_4:54546899-54546918	Msa0688490:intron	40.0%
	ATTGCCTTGTCGCACTATTA+AGG	+	chr4_4:54546906-54546925	None:intergenic	40.0%
	GACTAATGCAGTTGCAATTG+CGG	-	chr4_4:54546931-54546950	Msa0688490:intron	40.0%
!	GCCTACTCGTGTCTTTTAAT+TGG	-	chr4_4:54547181-54547200	Msa0688490:five_prime_UTR	40.0%
	GCTAACCAAGTCAATGAAGT+TGG	-	chr4_4:54547239-54547258	Msa0688490:five_prime_UTR	40.0%
!	TCTCTCCAACTTCATTGACT+TGG	+	chr4_4:54547247-54547266	None:intergenic	40.0%
!	TGATGATGCAAAGTGAAGTG+AGG	+	chr4_4:54545116-54545135	None:intergenic	40.0%
!!	AAGAAGTGTTTGTATGGGGA+TGG	-	chr4_4:54545265-54545284	Msa0688490:three_prime_UTR	40.0%
!	TTCTTCTAGTGGACAACAAC+AGG	-	chr4_4:54545289-54545308	Msa0688490:three_prime_UTR	40.0%
	TCATGAAGGACAAAGATGGA+AGG	+	chr4_4:54545398-54545417	None:intergenic	40.0%
!	TCTTCTTTGAAGAACACCCT+TGG	-	chr4_4:54545477-54545496	Msa0688490:CDS	40.0%
!	CTTCTTTGAAGAACACCCTT+GGG	-	chr4_4:54545478-54545497	Msa0688490:CDS	40.0%
	GCTGTATACATGTGGTTCAT+AGG	+	chr4_4:54545585-54545604	None:intergenic	40.0%
	ATGAACCACATGTATACAGC+TGG	-	chr4_4:54545585-54545604	Msa0688490:CDS	40.0%
	TGAACCACATGTATACAGCT+GGG	-	chr4_4:54545586-54545605	Msa0688490:CDS	40.0%
	TTGATGGAATAAAGTCAGGG+AGG	-	chr4_4:54545624-54545643	Msa0688490:CDS	40.0%
	GCAAATGTCTAACCAGTCAT+TGG	-	chr4_4:54546020-54546039	Msa0688490:CDS	40.0%
	TTAACATGCTCTCCAATGAC+TGG	+	chr4_4:54546035-54546054	None:intergenic	40.0%
!!!	CATTTTAGAGCTTCTGGTTG+AGG	+	chr4_4:54546096-54546115	None:intergenic	40.0%
!!!	CTTGGACATTTTAGAGCTTC+TGG	+	chr4_4:54546102-54546121	None:intergenic	40.0%
!!	GTGTTGTTTGAGTCACATCT+TGG	+	chr4_4:54546120-54546139	None:intergenic	40.0%
!	GCAAGTCTTGTAGGAGATAT+TGG	-	chr4_4:54546190-54546209	Msa0688490:CDS	40.0%
	AGGAGATATTGGACTAAAGG+TGG	-	chr4_4:54546201-54546220	Msa0688490:CDS	40.0%
	ACACTAAGGAATGTTCCTGT+TGG	-	chr4_4:54546225-54546244	Msa0688490:intron	40.0%
	TAAGGAATGTTCCTGTTGGT+GGG	-	chr4_4:54546229-54546248	Msa0688490:intron	40.0%
	CATCATCAGCTTCATCAAAG+AGG	-	chr4_4:54546274-54546293	Msa0688490:intron	40.0%
!!	GGATTTGGGTTGGTTTGAAA+TGG	+	chr4_4:54546312-54546331	None:intergenic	40.0%
!	GGAAACAAGTGAGTGGATTT+GGG	+	chr4_4:54546326-54546345	None:intergenic	40.0%
	ATTGAAGGGAAACAAGTGAG+TGG	+	chr4_4:54546333-54546352	None:intergenic	40.0%
	ATCTCACACTAGCATTAGCT+AGG	-	chr4_4:54546370-54546389	Msa0688490:intron	40.0%
	AGCATTAGCTAGGCTTCAAA+AGG	-	chr4_4:54546380-54546399	Msa0688490:intron	40.0%
	GCATTAGCTAGGCTTCAAAA+GGG	-	chr4_4:54546381-54546400	Msa0688490:intron	40.0%
	AAGAATACCACATGAGGTGT+TGG	+	chr4_4:54546457-54546476	None:intergenic	40.0%
	AACCATTCCTCAAACAACCA+TGG	-	chr4_4:54546492-54546511	Msa0688490:intron	40.0%
	ATCCATGGTTGTTTGAGGAA+TGG	+	chr4_4:54546497-54546516	None:intergenic	40.0%
	GAAGAATCCATGGTTGTTTG+AGG	+	chr4_4:54546502-54546521	None:intergenic	40.0%
	TAAGTGCTTCGAAGAATCCA+TGG	+	chr4_4:54546512-54546531	None:intergenic	40.0%
!	TGGATTCTTCGAAGCACTTA+TGG	-	chr4_4:54546512-54546531	Msa0688490:intron	40.0%
!	GGATTCTTCGAAGCACTTAT+GGG	-	chr4_4:54546513-54546532	Msa0688490:intron	40.0%
	GCAGAATCTTTACTACATGG+GGG	-	chr4_4:54546551-54546570	Msa0688490:intron	40.0%
	AATGGCAATGCTAATGGCAA+TGG	-	chr4_4:54546582-54546601	Msa0688490:intron	40.0%
!!!	GGGTTTTGTTAGGTTTTCCA+TGG	-	chr4_4:54546721-54546740	Msa0688490:intron	40.0%
	TCTCCATTATTGAACTGCCA+TGG	+	chr4_4:54546741-54546760	None:intergenic	40.0%
	ATGGATCATTTAGGAAGAGC+TGG	-	chr4_4:54546777-54546796	Msa0688490:intron	40.0%
	ATCATTTAGGAAGAGCTGGT+TGG	-	chr4_4:54546781-54546800	Msa0688490:intron	40.0%
	GAATGGTCTCACTTCATCTT+GGG	-	chr4_4:54546803-54546822	Msa0688490:intron	40.0%
	AATGGTCTCACTTCATCTTG+GGG	-	chr4_4:54546804-54546823	Msa0688490:intron	40.0%
	TGAACCTTAATAGTGCGACA+AGG	-	chr4_4:54546899-54546918	Msa0688490:intron	40.0%
	ATTGCCTTGTCGCACTATTA+AGG	+	chr4_4:54546906-54546925	None:intergenic	40.0%
	GACTAATGCAGTTGCAATTG+CGG	-	chr4_4:54546931-54546950	Msa0688490:intron	40.0%
!	GCCTACTCGTGTCTTTTAAT+TGG	-	chr4_4:54547181-54547200	Msa0688490:five_prime_UTR	40.0%
	GCTAACCAAGTCAATGAAGT+TGG	-	chr4_4:54547239-54547258	Msa0688490:five_prime_UTR	40.0%
!	TCTCTCCAACTTCATTGACT+TGG	+	chr4_4:54547247-54547266	None:intergenic	40.0%
!!	GTCGTACAAGATGATGGATG+AGG	+	chr4_4:54545007-54545026	None:intergenic	45.0%
!	ATGGGGATGGATTCTTCTAG+TGG	-	chr4_4:54545278-54545297	Msa0688490:three_prime_UTR	45.0%
	GAACCACATGTATACAGCTG+GGG	-	chr4_4:54545587-54545606	Msa0688490:CDS	45.0%
!	TTTAGAGCTTCTGGTTGAGG+AGG	+	chr4_4:54546093-54546112	None:intergenic	45.0%
	GGACTAAAGGTGGTACACTA+AGG	-	chr4_4:54546211-54546230	Msa0688490:intron	45.0%
	CTAAGGAATGTTCCTGTTGG+TGG	-	chr4_4:54546228-54546247	Msa0688490:intron	45.0%
	AAGGAATGTTCCTGTTGGTG+GGG	-	chr4_4:54546230-54546249	Msa0688490:intron	45.0%
!	ACAAGTGAGTGGATTTGGGT+TGG	+	chr4_4:54546322-54546341	None:intergenic	45.0%
!	GGGAAACAAGTGAGTGGATT+TGG	+	chr4_4:54546327-54546346	None:intergenic	45.0%
	AGGCTTCAAAAGGGTCAACT+TGG	-	chr4_4:54546390-54546409	Msa0688490:intron	45.0%
	AACCAAACCAACACCTCATG+TGG	-	chr4_4:54546447-54546466	Msa0688490:intron	45.0%
!!	TACCACATGAGGTGTTGGTT+TGG	+	chr4_4:54546452-54546471	None:intergenic	45.0%
	GAATCTTTACTACATGGGGG+AGG	-	chr4_4:54546554-54546573	Msa0688490:intron	45.0%
!!	TACATGGGGGAGGTTGATAA+TGG	-	chr4_4:54546564-54546583	Msa0688490:intron	45.0%
	TTAGGAAGAGCTGGTTGGAA+TGG	-	chr4_4:54546786-54546805	Msa0688490:intron	45.0%
	GGAATGGTCTCACTTCATCT+TGG	-	chr4_4:54546802-54546821	Msa0688490:intron	45.0%
	ATGGTCTCACTTCATCTTGG+GGG	-	chr4_4:54546805-54546824	Msa0688490:intron	45.0%
	ACATTAGGGGGCTATTGAGA+AGG	+	chr4_4:54546836-54546855	None:intergenic	45.0%
	ACGAGACGGTGTCTACATTA+GGG	+	chr4_4:54546850-54546869	None:intergenic	45.0%
!!	GTCGTACAAGATGATGGATG+AGG	+	chr4_4:54545007-54545026	None:intergenic	45.0%
!	ATGGGGATGGATTCTTCTAG+TGG	-	chr4_4:54545278-54545297	Msa0688490:three_prime_UTR	45.0%
	GAACCACATGTATACAGCTG+GGG	-	chr4_4:54545587-54545606	Msa0688490:CDS	45.0%
!	TTTAGAGCTTCTGGTTGAGG+AGG	+	chr4_4:54546093-54546112	None:intergenic	45.0%
	GGACTAAAGGTGGTACACTA+AGG	-	chr4_4:54546211-54546230	Msa0688490:intron	45.0%
	CTAAGGAATGTTCCTGTTGG+TGG	-	chr4_4:54546228-54546247	Msa0688490:intron	45.0%
	AAGGAATGTTCCTGTTGGTG+GGG	-	chr4_4:54546230-54546249	Msa0688490:intron	45.0%
!	ACAAGTGAGTGGATTTGGGT+TGG	+	chr4_4:54546322-54546341	None:intergenic	45.0%
!	GGGAAACAAGTGAGTGGATT+TGG	+	chr4_4:54546327-54546346	None:intergenic	45.0%
	AGGCTTCAAAAGGGTCAACT+TGG	-	chr4_4:54546390-54546409	Msa0688490:intron	45.0%
	AACCAAACCAACACCTCATG+TGG	-	chr4_4:54546447-54546466	Msa0688490:intron	45.0%
!!	TACCACATGAGGTGTTGGTT+TGG	+	chr4_4:54546452-54546471	None:intergenic	45.0%
	GAATCTTTACTACATGGGGG+AGG	-	chr4_4:54546554-54546573	Msa0688490:intron	45.0%
!!	TACATGGGGGAGGTTGATAA+TGG	-	chr4_4:54546564-54546583	Msa0688490:intron	45.0%
	TTAGGAAGAGCTGGTTGGAA+TGG	-	chr4_4:54546786-54546805	Msa0688490:intron	45.0%
	GGAATGGTCTCACTTCATCT+TGG	-	chr4_4:54546802-54546821	Msa0688490:intron	45.0%
	ATGGTCTCACTTCATCTTGG+GGG	-	chr4_4:54546805-54546824	Msa0688490:intron	45.0%
	ACATTAGGGGGCTATTGAGA+AGG	+	chr4_4:54546836-54546855	None:intergenic	45.0%
	ACGAGACGGTGTCTACATTA+GGG	+	chr4_4:54546850-54546869	None:intergenic	45.0%
!!	AAAAATTGAAAATTTATTAA+TGG	-	chr4_4:54545713-54545732	Msa0688490:CDS	5.0%
!!	AAAAATTGAAAATTTATTAA+TGG	-	chr4_4:54545713-54545732	Msa0688490:CDS	5.0%
!!	GCTTCTGGTTGAGGAGGTTT+TGG	+	chr4_4:54546087-54546106	None:intergenic	50.0%
!	AGGAATGTTCCTGTTGGTGG+GGG	-	chr4_4:54546231-54546250	Msa0688490:intron	50.0%
	GAGACGGTGTCTACATTAGG+GGG	+	chr4_4:54546848-54546867	None:intergenic	50.0%
	CGAGACGGTGTCTACATTAG+GGG	+	chr4_4:54546849-54546868	None:intergenic	50.0%
	CACGAGACGGTGTCTACATT+AGG	+	chr4_4:54546851-54546870	None:intergenic	50.0%
	TGCGGCTGCAATTACGGTTA+TGG	-	chr4_4:54546993-54547012	Msa0688490:five_prime_UTR	50.0%
!!	GCTTCTGGTTGAGGAGGTTT+TGG	+	chr4_4:54546087-54546106	None:intergenic	50.0%
!	AGGAATGTTCCTGTTGGTGG+GGG	-	chr4_4:54546231-54546250	Msa0688490:intron	50.0%
	GAGACGGTGTCTACATTAGG+GGG	+	chr4_4:54546848-54546867	None:intergenic	50.0%
	CGAGACGGTGTCTACATTAG+GGG	+	chr4_4:54546849-54546868	None:intergenic	50.0%
	CACGAGACGGTGTCTACATT+AGG	+	chr4_4:54546851-54546870	None:intergenic	50.0%
	TGCGGCTGCAATTACGGTTA+TGG	-	chr4_4:54546993-54547012	Msa0688490:five_prime_UTR	50.0%
	AGGCCCCAGCTGTATACATG+TGG	+	chr4_4:54545593-54545612	None:intergenic	55.0%
!	GGAATGTTCCTGTTGGTGGG+GGG	-	chr4_4:54546232-54546251	Msa0688490:intron	55.0%
	TTTCTACACCCCCCACCAAC+AGG	+	chr4_4:54546243-54546262	None:intergenic	55.0%
	CTCACTTCATCTTGGGGGCA+TGG	-	chr4_4:54546810-54546829	Msa0688490:intron	55.0%
	AGGCCCCAGCTGTATACATG+TGG	+	chr4_4:54545593-54545612	None:intergenic	55.0%
!	GGAATGTTCCTGTTGGTGGG+GGG	-	chr4_4:54546232-54546251	Msa0688490:intron	55.0%
	TTTCTACACCCCCCACCAAC+AGG	+	chr4_4:54546243-54546262	None:intergenic	55.0%
	CTCACTTCATCTTGGGGGCA+TGG	-	chr4_4:54546810-54546829	Msa0688490:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	54544978	54547271	54544978	ID=Msa0688490;Name=Msa0688490
chr4_4	mRNA	54544978	54547271	54544978	ID=Msa0688490-mRNA-1;Parent=Msa0688490;Name=Msa0688490-mRNA-1;_AED=0.00;_eAED=0.00;_QI=300\|1\|1\|1\|1\|1\|2\|414\|289
chr4_4	exon	54544978	54546228	54544978	ID=Msa0688490-mRNA-1:exon:14363;Parent=Msa0688490-mRNA-1
chr4_4	exon	54546939	54547271	54546939	ID=Msa0688490-mRNA-1:exon:14362;Parent=Msa0688490-mRNA-1
chr4_4	five_prime_UTR	54546972	54547271	54546972	ID=Msa0688490-mRNA-1:five_prime_utr;Parent=Msa0688490-mRNA-1
chr4_4	CDS	54546939	54546971	54546939	ID=Msa0688490-mRNA-1:cds;Parent=Msa0688490-mRNA-1
chr4_4	CDS	54545392	54546228	54545392	ID=Msa0688490-mRNA-1:cds;Parent=Msa0688490-mRNA-1
chr4_4	three_prime_UTR	54544978	54545391	54544978	ID=Msa0688490-mRNA-1:three_prime_utr;Parent=Msa0688490-mRNA-1

Gene Sequence

>Msa0688490

ATGGGGATGGATTCTTCTAGTGGACAACAACAGCAAATGTCTAACCAGTCATTGGAGAGCATGTTAACATGTTCAAAAGCAGAACAAGACAAAAAGCCAAAACCTCCTCAACCAGAAGCTCTAAAATGTCCAAGATGTGACTCAAACAACACCAAATTTTGTTACTACAACAATTACAGTCTTTCTCAACCAAGATATTTCTGCAAGTCTTGTAGGAGATATTGGACTAAAGGTGGTACACTAAGGAATGTTCCTGTTGGTGGGGGGTGTAGAAAGAACAAAAGATCATCATCAGCTTCATCAAAGAGGTCTCAAGATCAACCATTTCAAACCAACCCAAATCCACTCACTTGTTTCCCTTCAATGTCCTATGATTCCAATGATCTCACACTAGCATTAGCTAGGCTTCAAAAGGGTCAACTTGGATTTGATCATGAACATGATTTTTCCATTTTAGGAAACCAAACCAACACCTCATGTGGTATTCTTAACAATCATGGTATGAACCATTCCTCAAACAACCATGGATTCTTCGAAGCACTTATGGGATCTCAAAACAATGTGCAGAATCTTTACTACATGGGGGAGGTTGATAATGGCAATGCTAATGGCAATGGTAATGGTGAGATGATGTTACCTTATGATCATGAAATGAGCATTGCAACTACTCAAGCAGTTACAGTTACTACTATGAAACAAGAAATGTGTAATGTTAGGGAACAAAATGAAAATAGGGTTTTGTTAGGTTTTCCATGGCAGTTCAATAATGGAGACACAAATATGGCTGAAATGGATCATTTAGGAAGAGCTGGTTGGAATGGTCTCACTTCATCTTGGGGGCATGGCCTTCTCAATAGCCCCCTAATGTAG

Protein sequence

>Msa0688490

MGMDSSSGQQQQMSNQSLESMLTCSKAEQDKKPKPPQPEALKCPRCDSNNTKFCYYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGGCRKNKRSSSASSKRSQDQPFQTNPNPLTCFPSMSYDSNDLTLALARLQKGQLGFDHEHDFSILGNQTNTSCGILNNHGMNHSSNNHGFFEALMGSQNNVQNLYYMGEVDNGNANGNGNGEMMLPYDHEMSIATTQAVTVTTMKQEMCNVREQNENRVLLGFPWQFNNGDTNMAEMDHLGRAGWNGLTSSWGHGLLNSPLM*