AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0694740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0694740 MtrunA17_Chr4g0045641 94.757 534 10 1 36 551 1 534 0.0 1018
Msa0694740 MtrunA17_Chr6g0462191 33.811 349 162 15 175 491 101 412 2.13e-29 120
Msa0694740 MtrunA17_Chr7g0263731 32.493 357 170 14 181 491 108 439 2.15e-26 112
Msa0694740 MtrunA17_Chr1g0181581 29.261 352 181 11 179 491 114 436 3.07e-26 112
Msa0694740 MtrunA17_Chr1g0200161 34.054 185 95 4 179 344 139 315 8.00e-25 107
Msa0694740 MtrunA17_Chr4g0020671 30.000 260 125 8 38 294 4 209 1.06e-24 107
Msa0694740 MtrunA17_Chr7g0273861 27.273 330 179 10 177 493 121 402 6.21e-23 101
Msa0694740 MtrunA17_Chr8g0346021 26.476 525 275 18 36 489 1 485 1.05e-22 102
Msa0694740 MtrunA17_Chr6g0472551 25.868 317 187 9 177 467 113 407 2.14e-17 85.1
Msa0694740 MtrunA17_Chr4g0025441 31.193 327 179 15 183 496 98 391 6.06e-15 77.4
Msa0694740 MtrunA17_Chr8g0389781 41.667 84 43 1 179 256 124 207 1.01e-13 73.6
Msa0694740 MtrunA17_Chr8g0371131 50.000 62 31 0 183 244 73 134 1.98e-13 67.8
Msa0694740 MtrunA17_Chr7g0273951 46.970 66 35 0 183 248 108 173 2.48e-13 72.0
Msa0694740 MtrunA17_Chr4g0060381 47.619 63 33 0 183 245 102 164 4.13e-13 72.0
Msa0694740 MtrunA17_Chr2g0330921 51.613 62 30 0 182 243 98 159 6.46e-13 70.5
Msa0694740 MtrunA17_Chr1g0204901 41.176 68 40 0 180 247 135 202 2.34e-12 69.3
Msa0694740 MtrunA17_Chr3g0135851 26.099 364 190 12 146 462 55 386 2.68e-12 68.9
Msa0694740 MtrunA17_Chr4g0057321 50.820 61 30 0 183 243 90 150 6.11e-12 67.8
Msa0694740 MtrunA17_Chr8g0340761 45.902 61 33 0 183 243 128 188 8.37e-12 67.4
Msa0694740 MtrunA17_Chr3g0126901 56.250 64 28 0 183 246 114 177 1.22e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0694740 AT3G59690.2 48.768 568 186 19 36 550 1 516 3.71e-143 423
Msa0694740 AT3G59690.1 48.768 568 186 19 36 550 1 516 3.71e-143 423
Msa0694740 AT2G43680.4 49.446 451 136 17 127 551 284 668 5.05e-108 338
Msa0694740 AT2G43680.4 58.696 138 41 5 36 161 1 134 2.23e-25 110
Msa0694740 AT2G43680.2 49.446 451 136 17 127 551 284 668 5.05e-108 338
Msa0694740 AT2G43680.2 58.696 138 41 5 36 161 1 134 2.23e-25 110
Msa0694740 AT2G43680.3 49.446 451 136 17 127 551 284 668 5.05e-108 338
Msa0694740 AT2G43680.3 58.696 138 41 5 36 161 1 134 2.23e-25 110
Msa0694740 AT2G43680.5 49.446 451 136 17 127 551 284 668 5.05e-108 338
Msa0694740 AT2G43680.5 58.696 138 41 5 36 161 1 134 2.23e-25 110
Msa0694740 AT2G43680.1 49.446 451 136 17 127 551 285 669 5.82e-108 337
Msa0694740 AT2G43680.1 55.396 139 45 5 36 161 1 135 1.76e-25 111
Msa0694740 AT3G52290.1 31.046 306 159 10 180 460 107 385 5.79e-29 119
Msa0694740 AT5G03040.1 27.701 509 245 19 36 494 1 436 2.89e-28 118
Msa0694740 AT5G03040.3 27.701 509 245 19 36 494 1 436 2.89e-28 118
Msa0694740 AT5G03040.2 27.701 509 245 19 36 494 1 436 2.89e-28 118
Msa0694740 AT1G01110.2 27.879 495 245 20 36 466 1 447 6.35e-26 112
Msa0694740 AT5G13460.1 28.221 326 158 11 153 466 90 351 2.63e-23 103
Msa0694740 AT4G00820.1 28.889 360 190 13 180 490 131 473 1.25e-21 99.0
Msa0694740 AT3G49260.1 28.428 299 163 6 183 458 122 392 4.54e-21 96.7
Msa0694740 AT3G49260.4 28.428 299 163 6 183 458 122 392 4.54e-21 96.7
Msa0694740 AT3G49260.2 28.428 299 163 6 183 458 122 392 4.54e-21 96.7
Msa0694740 AT3G49260.3 27.425 299 167 5 183 458 122 393 5.57e-21 96.7
Msa0694740 AT2G26410.2 32.461 191 85 8 173 336 140 313 6.79e-16 80.9
Msa0694740 AT2G26410.1 32.461 191 85 8 173 336 140 313 6.79e-16 80.9
Msa0694740 AT3G09710.1 34.706 170 84 6 180 331 107 267 1.54e-15 79.7
Msa0694740 AT2G26180.1 32.821 195 111 7 183 358 86 279 7.56e-15 77.0
Msa0694740 AT3G09710.2 33.333 177 91 6 180 331 107 281 1.33e-14 76.6
Msa0694740 AT4G10640.1 26.582 316 165 10 175 466 94 366 1.44e-14 76.3
Msa0694740 AT4G14750.1 45.588 68 37 0 180 247 104 171 6.42e-14 73.9
Msa0694740 AT3G22190.3 34.078 179 89 5 183 342 90 258 6.55e-14 74.3
Msa0694740 AT3G22190.2 34.078 179 89 5 183 342 90 258 6.55e-14 74.3
Msa0694740 AT3G22190.4 34.078 179 89 5 183 342 90 258 6.55e-14 74.3
Msa0694740 AT3G22190.1 34.078 179 89 5 183 342 90 258 6.55e-14 74.3
Msa0694740 AT4G14750.3 47.692 65 34 0 183 247 166 230 9.47e-14 73.9
Msa0694740 AT1G18840.1 31.410 156 80 6 180 326 108 245 1.02e-13 74.3
Msa0694740 AT1G18840.3 31.410 156 80 6 180 326 108 245 1.02e-13 74.3
Msa0694740 AT1G18840.2 31.410 156 80 6 180 326 108 245 1.02e-13 74.3
Msa0694740 AT4G14750.2 47.692 65 34 0 183 247 166 230 1.08e-13 73.9
Msa0694740 AT5G62070.1 51.786 56 27 0 180 235 116 171 2.22e-13 72.4
Msa0694740 AT3G16490.1 48.387 62 32 0 183 244 109 170 2.31e-13 72.4
Msa0694740 AT5G07240.1 46.774 62 33 0 180 241 105 166 2.84e-13 72.4
Msa0694740 AT5G07240.2 46.774 62 33 0 180 241 151 212 3.33e-13 72.4
Msa0694740 AT1G17480.1 47.541 61 32 0 183 243 96 156 5.27e-13 71.2
Msa0694740 AT1G74690.1 30.921 152 86 4 180 326 112 249 8.42e-13 71.6
Msa0694740 AT3G49380.1 37.634 93 55 1 180 269 109 201 1.12e-12 70.1
Msa0694740 AT3G51380.1 42.857 70 40 0 175 244 31 100 1.24e-12 64.7
Msa0694740 AT2G33990.1 29.048 210 124 8 183 380 49 245 1.36e-12 68.6
Msa0694740 AT5G03960.3 27.723 303 162 10 181 472 108 364 2.81e-12 69.3
Msa0694740 AT5G03960.2 27.723 303 162 10 181 472 108 364 2.81e-12 69.3
Msa0694740 AT5G03960.1 27.723 303 162 10 181 472 107 363 2.83e-12 69.3
Msa0694740 AT1G51960.1 42.857 77 39 1 183 259 102 173 4.57e-12 68.2
Msa0694740 AT4G29150.1 43.750 64 36 0 180 243 130 193 7.14e-12 67.8
Msa0694740 AT1G72670.1 43.077 65 37 0 177 241 89 153 7.95e-12 67.8

Find 153 sgRNAs with CRISPR-Local

Find 298 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAGCATTGAGAAAATATT+TGG 0.147656 4_4:+64092636 Msa0694740:CDS
ATAGTCGATCTTGTTGATTT+TGG 0.221689 4_4:-64094299 None:intergenic
AATCACACAAATCTTGTAAA+AGG 0.233035 4_4:-64094797 None:intergenic
GGCTTTCTTTCAACCCTTGC+AGG 0.240511 4_4:-64094776 None:intergenic
ACAGGCGTTTGAGTTGATTT+TGG 0.283729 4_4:-64094074 None:intergenic
TTTCCTTGTGGAGTTCTAAA+TGG 0.286373 4_4:-64094353 None:intergenic
TCAAGTGACTGGTATTTATC+TGG 0.291478 4_4:-64094713 None:intergenic
GTCAATGTTCTCTAGAAATA+AGG 0.300536 4_4:+64094667 Msa0694740:CDS
TTTCCTTTGTCACAAGGAAT+AGG 0.310912 4_4:+64094623 Msa0694740:CDS
GGGTCATTCAAATGGAATAA+AGG 0.327034 4_4:+64094644 Msa0694740:CDS
GTGTGAATTGGAAATTCTTC+AGG 0.333924 4_4:-64094184 None:intergenic
CTCTTGGAATCAGTGCTTGT+TGG 0.334476 4_4:-64094590 None:intergenic
TGAGAGCTTGAGCCAGGTCT+TGG 0.341364 4_4:-64094239 None:intergenic
TACCAGTCACTTGAATCTAT+TGG 0.350119 4_4:+64094722 Msa0694740:CDS
ACAGTTGGAGCCATGTAGTT+TGG 0.351130 4_4:-64094491 None:intergenic
GGCTGCTTACAGAGGTTATA+TGG 0.351906 4_4:+64092932 Msa0694740:CDS
CACACCACCAAGACCATATT+CGG 0.361031 4_4:+64094202 Msa0694740:CDS
TTCCTTTGTCACAAGGAATA+GGG 0.362580 4_4:+64094624 Msa0694740:CDS
CAGAGCTCTAAAACTCTTCC+TGG 0.366223 4_4:-64093249 None:intergenic
TTCCGCGAAGATGCCGCTTT+CGG 0.379549 4_4:-64092831 None:intergenic
GCAGCATTGAGAAAATATTT+GGG 0.385983 4_4:+64092637 Msa0694740:CDS
TGGTTCTGTAAAGTTGGTTC+TGG 0.389882 4_4:-64092885 None:intergenic
TAACAGCTGCATGTGTTTCA+TGG 0.394012 4_4:-64093336 None:intergenic
CGGTACATCGAAAGGTGAAT+TGG 0.396785 4_4:-64094423 None:intergenic
ATGGTCTTGGTGGTGTGAAT+TGG 0.398616 4_4:-64094196 None:intergenic
GGTCATTCAAATGGAATAAA+GGG 0.400507 4_4:+64094645 Msa0694740:CDS
CTAAATGGAGGAGTTCTAGA+TGG 0.403634 4_4:-64094338 None:intergenic
ACCTTTCGATGTACCGTTGA+AGG 0.404025 4_4:+64094430 Msa0694740:CDS
GGCCTTTGAGAGCTTGAGCC+AGG 0.404316 4_4:-64094245 None:intergenic
AAAGGTTTGGTAAGGCTTCA+AGG 0.407441 4_4:+64093272 Msa0694740:CDS
AATGAAGCTGGAAGTACCTT+GGG 0.409619 4_4:+64093437 Msa0694740:CDS
TGCTGCTGAGATGGGAAAGA+AGG 0.411748 4_4:+64091669 Msa0694740:CDS
AACAACAAGTACTTGCAATT+AGG 0.413110 4_4:+64092673 Msa0694740:CDS
CCACAGCCAAATCTTTCTCT+TGG 0.416854 4_4:-64094548 None:intergenic
ACCTTGGGGAAAAGTGCTTT+AGG 0.429209 4_4:+64093452 Msa0694740:CDS
TTAAACTTACAGGAAGAAAC+AGG 0.431182 4_4:+64091644 Msa0694740:intron
TTATAGCATAGCAACACATT+AGG 0.434484 4_4:-64095028 None:intergenic
AGGCAGGTAACAATGAAGAT+TGG 0.438407 4_4:+64093647 Msa0694740:CDS
AGTAATCCAAGAGAAAGATT+TGG 0.440973 4_4:+64094542 Msa0694740:CDS
TTTGGTCGCTCGGGAGGTGT+GGG 0.442251 4_4:-64092699 None:intergenic
AAGGAATAGGGTCATTCAAA+TGG 0.447925 4_4:+64094636 Msa0694740:CDS
GCCCTTGGAGATGGATCCCT+TGG 0.448654 4_4:-64092786 None:intergenic
ACTCGGCTTGATATCGTGCC+TGG 0.450049 4_4:-64093409 None:intergenic
TATGATTGAGAGTGTATTAG+TGG 0.456025 4_4:+64094897 Msa0694740:three_prime_UTR
AGGAAATGAGACTCTCCTCT+TGG 0.466551 4_4:-64094606 None:intergenic
AAACAGGAATGCTGCTGAGA+TGG 0.468508 4_4:+64091660 Msa0694740:CDS
GGCAGGTAACAATGAAGATT+GGG 0.469502 4_4:+64093648 Msa0694740:CDS
GGCTTAGGAGAAGGAGGCCT+AGG 0.481446 4_4:-64092756 None:intergenic
TCCAGCTTCATTCTTGTACT+CGG 0.482184 4_4:-64093426 None:intergenic
GATTCCCATGGTGGTGGAAC+TGG 0.486021 4_4:+64094117 Msa0694740:CDS
GAGCTCTGAAAGGTTTGGTA+AGG 0.487495 4_4:+64093264 Msa0694740:CDS
GCGCGATGTCAAAATATTCA+AGG 0.487546 4_4:+64094384 Msa0694740:CDS
CTCCGAAAGCGGCATCTTCG+CGG 0.487625 4_4:+64092829 Msa0694740:CDS
GGAGAAGCGGCCCTTGGAGA+TGG 0.488016 4_4:-64092795 None:intergenic
AGATGGGAAAGAAGGGGAGT+TGG 0.488180 4_4:+64091677 Msa0694740:CDS
TGCTGCTTGGTTCTCTGAAG+AGG 0.488717 4_4:-64092620 None:intergenic
TCTTTCAACCCTTGCAGGCA+AGG 0.489990 4_4:-64094771 None:intergenic
TCCCTTGGAGAAGGAGGCTT+AGG 0.492264 4_4:-64092771 None:intergenic
TGTCTCGGCGACTAAGATCC+AGG 0.493274 4_4:+64092911 Msa0694740:CDS
CTCCTAAGCCTCCTTCTCCA+AGG 0.498883 4_4:+64092769 Msa0694740:CDS
AACAGGAATGCTGCTGAGAT+GGG 0.501119 4_4:+64091661 Msa0694740:CDS
GCCGAGTACAAGAATGAAGC+TGG 0.501793 4_4:+64093425 Msa0694740:CDS
CTCCTAGAGTTGCTTCTCCT+AGG 0.502361 4_4:+64092739 Msa0694740:CDS
ACATGCTGGTTCTGTAAAGT+TGG 0.510843 4_4:-64092891 None:intergenic
ACTCGCGGAGAAGCGGCCCT+TGG 0.513311 4_4:-64092801 None:intergenic
GAATGAAGCTGGAAGTACCT+TGG 0.515373 4_4:+64093436 Msa0694740:CDS
AGGCTTCAAGGAGTGGTAAG+AGG 0.518282 4_4:+64093284 Msa0694740:CDS
GGTCGCTCGGGAGGTGTGGG+AGG 0.522529 4_4:-64092696 None:intergenic
TATTCAAGGCCAAGGGGAGT+AGG 0.527231 4_4:+64094398 Msa0694740:CDS
AGCACAAAAGAGAAGAAGAA+AGG 0.528405 4_4:+64092555 Msa0694740:CDS
CCAACCAGTTCCACCACCAT+GGG 0.530319 4_4:-64094121 None:intergenic
GAATTGGATCCTACTCCCCT+TGG 0.530551 4_4:-64094407 None:intergenic
CTCGATCGCGTATCTGTCAC+AGG 0.530625 4_4:-64094092 None:intergenic
GACCCTATTCCTTGTGACAA+AGG 0.531331 4_4:-64094626 None:intergenic
TACACATCACTCTAAGGGAA+AGG 0.537172 4_4:+64091723 Msa0694740:CDS
TTCCAATAGATTCAAGTGAC+TGG 0.538161 4_4:-64094724 None:intergenic
GCTGCTGAGATGGGAAAGAA+GGG 0.540744 4_4:+64091670 Msa0694740:CDS
TCCTAAGCCTCCTTCTCCAA+GGG 0.542552 4_4:+64092770 Msa0694740:CDS
AGCAGTATCCTTGCCTGCAA+GGG 0.545645 4_4:+64094763 Msa0694740:CDS
ATTACTACTAGCGCGTACTT+TGG 0.546568 4_4:-64094525 None:intergenic
GCTGCTTGGTTCTCTGAAGA+GGG 0.546752 4_4:-64092619 None:intergenic
GTCTCATTTCCTTTGTCACA+AGG 0.546913 4_4:+64094617 Msa0694740:CDS
AGAAGCGAGGTTGCAGAGGA+AGG 0.547317 4_4:+64093699 Msa0694740:CDS
TCCATGTTATCAAAGGCAAA+AGG 0.548096 4_4:-64094266 None:intergenic
TCCTTCAACGGTACATCGAA+AGG 0.548425 4_4:-64094431 None:intergenic
TGTCAAAATATTCAAGGCCA+AGG 0.548648 4_4:+64094390 Msa0694740:CDS
CGAGCGGATTCCCATGGTGG+TGG 0.550081 4_4:+64094111 Msa0694740:CDS
TCTTAGTCGCCGAGACATGC+TGG 0.550419 4_4:-64092905 None:intergenic
TTGGCCTTGGCAGAAACAGT+TGG 0.550995 4_4:-64094506 None:intergenic
TGATAGCTTACTGACAAAAG+AGG 0.552866 4_4:+64093672 Msa0694740:CDS
AAGTAGAAGCGAGGTTGCAG+AGG 0.554875 4_4:+64093695 Msa0694740:CDS
CAGCAGTATCCTTGCCTGCA+AGG 0.555494 4_4:+64094762 Msa0694740:CDS
GATGGATCCCTTGGAGAAGG+AGG 0.557622 4_4:-64092777 None:intergenic
AATCCAGATGTTAGAAAACC+AGG 0.559159 4_4:+64093391 Msa0694740:CDS
ACTAGCGCGTACTTTGGCCT+TGG 0.561095 4_4:-64094519 None:intergenic
AGGATTCTCATGGCTCTCAC+AGG 0.561873 4_4:+64094687 Msa0694740:CDS
TGTTCCGAATATGGTCTTGG+TGG 0.562203 4_4:-64094206 None:intergenic
GCGCTTGAAGTGTTTCCTTG+TGG 0.562520 4_4:-64094365 None:intergenic
GTCAAAATATTCAAGGCCAA+GGG 0.562606 4_4:+64094391 Msa0694740:CDS
CTAGGAGAAGCAACTCTAGG+AGG 0.564157 4_4:-64092738 None:intergenic
TATAACCTCTGTAAGCAGCC+TGG 0.564353 4_4:-64092929 None:intergenic
AAAGAGAAGAAGAAAGGTGT+AGG 0.565399 4_4:+64092561 Msa0694740:CDS
GGCTCCAACTGTTTCTGCCA+AGG 0.565988 4_4:+64094502 Msa0694740:CDS
CCTTGTGGAGTTCTAAATGG+AGG 0.567299 4_4:-64094350 None:intergenic
ATGTAGTTTGGAACGGAGAA+CGG 0.569098 4_4:-64094479 None:intergenic
GGGAAAAGTGCTTTAGGACA+TGG 0.569234 4_4:+64093458 Msa0694740:CDS
TTTGGTAAGGCTTCAAGGAG+TGG 0.571343 4_4:+64093277 Msa0694740:CDS
GGAGATGGATCCCTTGGAGA+AGG 0.577659 4_4:-64092780 None:intergenic
TGCTTGGTTCTCTGAAGAGG+GGG 0.580393 4_4:-64092617 None:intergenic
CTCCAAGGGATCCATCTCCA+AGG 0.580760 4_4:+64092784 Msa0694740:CDS
GGTGTAGGAAAACTAAAGCA+TGG 0.583060 4_4:+64092576 Msa0694740:CDS
AGAGGTAACTCGCGGAGAAG+CGG 0.585392 4_4:-64092808 None:intergenic
TCCAACCAGTTCCACCACCA+TGG 0.585925 4_4:-64094122 None:intergenic
TGGTGTATCCATGTTATCAA+AGG 0.590388 4_4:-64094273 None:intergenic
GGAGAAGGAGGCTTAGGAGA+AGG 0.593364 4_4:-64092765 None:intergenic
TGGAGCCATGTAGTTTGGAA+CGG 0.593443 4_4:-64094486 None:intergenic
GGCCTAGGAGAAGCAACTCT+AGG 0.595533 4_4:-64092741 None:intergenic
GACCTGGCTCAAGCTCTCAA+AGG 0.598818 4_4:+64094243 Msa0694740:CDS
TCCAAGGGATCCATCTCCAA+GGG 0.601031 4_4:+64092785 Msa0694740:CDS
TAGGAGAAGCAACTCTAGGA+GGG 0.605104 4_4:-64092737 None:intergenic
CCTCCATTTAGAACTCCACA+AGG 0.606509 4_4:+64094350 Msa0694740:CDS
GAAGGAGGCTTAGGAGAAGG+AGG 0.609274 4_4:-64092762 None:intergenic
CCAAGAGAAAGATTTGGCTG+TGG 0.609282 4_4:+64094548 Msa0694740:CDS
TTTACAGAACCAGCATGTCT+CGG 0.610936 4_4:+64092896 Msa0694740:CDS
TCTAGAAATAAGGATTCTCA+TGG 0.612036 4_4:+64094677 Msa0694740:CDS
GTTCTCCGTTCCAAACTACA+TGG 0.616839 4_4:+64094481 Msa0694740:CDS
CGCGATCGAGCGGATTCCCA+TGG 0.617077 4_4:+64094105 Msa0694740:CDS
ATCTATTGGAAATGTGAGTG+TGG 0.620035 4_4:+64094736 Msa0694740:CDS
ACTCTAGGAGGGACGAAAGG+TGG 0.620082 4_4:-64092726 None:intergenic
ACTATGTTAACAGTTATGGA+AGG 0.620863 4_4:+64094046 Msa0694740:intron
AGACTATCATCATCCTTCAA+CGG 0.623430 4_4:-64094443 None:intergenic
CAAAAGACAAGTCCAAGACC+TGG 0.632412 4_4:+64094227 Msa0694740:CDS
CAGCATTGAGAAAATATTTG+GGG 0.632868 4_4:+64092638 Msa0694740:CDS
GCGAGTTACCTCTCCGAAAG+CGG 0.633756 4_4:+64092818 Msa0694740:CDS
GATCGAGCGGATTCCCATGG+TGG 0.638826 4_4:+64094108 Msa0694740:CDS
CTGCTTGGTTCTCTGAAGAG+GGG 0.639910 4_4:-64092618 None:intergenic
AGATTTGGCTGTGGTAGTAG+TGG 0.641972 4_4:+64094557 Msa0694740:CDS
GCAACTCTAGGAGGGACGAA+AGG 0.644669 4_4:-64092729 None:intergenic
AAGCTCTGTGACAACTAACA+AGG 0.646533 4_4:+64090758 Msa0694740:CDS
AAGATCCAGGCTGCTTACAG+AGG 0.646831 4_4:+64092924 Msa0694740:CDS
TCAAAATATTCAAGGCCAAG+GGG 0.647362 4_4:+64094392 Msa0694740:CDS
TAAGAGGACAGAATGTGAAG+AGG 0.651089 4_4:+64093300 Msa0694740:CDS
CAACAAGCACTGATTCCAAG+AGG 0.666446 4_4:+64094591 Msa0694740:CDS
GTGACAGATACGCGATCGAG+CGG 0.669089 4_4:+64094095 Msa0694740:CDS
CCCATGGTGGTGGAACTGGT+TGG 0.672536 4_4:+64094121 Msa0694740:CDS
CGAAGATGCCGCTTTCGGAG+AGG 0.682021 4_4:-64092826 None:intergenic
ATGAAGCTGGAAGTACCTTG+GGG 0.697268 4_4:+64093438 Msa0694740:CDS
GCGGAATGTTCATCAACACA+AGG 0.697723 4_4:+64092848 Msa0694740:CDS
CTGCTGAGATGGGAAAGAAG+GGG 0.698156 4_4:+64091671 Msa0694740:CDS
CAAAAGAGGAAGTAGAAGCG+AGG 0.699187 4_4:+64093686 Msa0694740:CDS
TAGTTTGGAACGGAGAACGG+TGG 0.711322 4_4:-64094476 None:intergenic
GCTTTCGGAGAGGTAACTCG+CGG 0.724827 4_4:-64092816 None:intergenic
GAATGTTCATCAACACAAGG+AGG 0.727547 4_4:+64092851 Msa0694740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTCTTTTTTACTTA+AGG - chr4_4:64091451-64091470 None:intergenic 10.0%
!! AAATTAAGATATAAGATTTA+AGG - chr4_4:64091626-64091645 None:intergenic 10.0%
!!! AATTTTCTTAATATTATCAA+AGG - chr4_4:64092093-64092112 None:intergenic 10.0%
!!! CTAATTTATTTTTGATAATT+TGG + chr4_4:64093607-64093626 Msa0694740:intron 10.0%
!!! TTTTATTTATGCTTTTATTT+TGG + chr4_4:64093799-64093818 Msa0694740:intron 10.0%
!!! TTTTTTTTATTAGTTTACAA+TGG - chr4_4:64094949-64094968 None:intergenic 10.0%
!! TTGGATATAATTGAATTAAA+AGG - chr4_4:64090990-64091009 None:intergenic 15.0%
!! AAAAATGAAAGATGAATTAT+TGG - chr4_4:64091128-64091147 None:intergenic 15.0%
!! ATATCTTAATTTAAACTTAC+AGG + chr4_4:64091634-64091653 Msa0694740:intron 15.0%
!!! ATTATTTGAAATATTGCAAA+AGG + chr4_4:64092250-64092269 Msa0694740:intron 15.0%
!! TAAGATAAGTATTAAGTATT+TGG + chr4_4:64093118-64093137 Msa0694740:intron 15.0%
!!! GTTACTTTATATAAAATTGA+TGG - chr4_4:64093514-64093533 None:intergenic 15.0%
!!! AATTAGTCTGATTTATATAA+AGG - chr4_4:64093593-64093612 None:intergenic 15.0%
!!! ATTTTTGTTTCGATTAAAAT+AGG - chr4_4:64093846-64093865 None:intergenic 15.0%
!! AAAATCTTAGATTTAGAAAT+GGG - chr4_4:64093878-64093897 None:intergenic 15.0%
!! AAAAATCTTAGATTTAGAAA+TGG - chr4_4:64093879-64093898 None:intergenic 15.0%
!! AAACATTAGACAAATAAAAA+TGG - chr4_4:64094841-64094860 None:intergenic 15.0%
!!! TTTTTATTTGTCTAATGTTT+TGG + chr4_4:64094841-64094860 Msa0694740:three_prime_UTR 15.0%
!! AAAAACATAATAGGAAATAA+TGG + chr4_4:64094969-64094988 Msa0694740:three_prime_UTR 15.0%
!!! ATTTGAAGATATAGCTTTTA+AGG + chr4_4:64090829-64090848 Msa0694740:intron 20.0%
!!! TTTTTTAATTGATCATGTGA+TGG + chr4_4:64091177-64091196 Msa0694740:intron 20.0%
!!! TGTGTGTGTTTTAATATATT+TGG + chr4_4:64091505-64091524 Msa0694740:intron 20.0%
!! TATTGAATTCCTCATATATT+TGG + chr4_4:64091548-64091567 Msa0694740:intron 20.0%
!!! TCTTATAATTGGTTTTCTTA+TGG + chr4_4:64091832-64091851 Msa0694740:intron 20.0%
!! ATAACTTAAAACAACTTCAA+AGG - chr4_4:64092446-64092465 None:intergenic 20.0%
!!! AAGTATTTGGTTTCTAAATT+TGG + chr4_4:64093131-64093150 Msa0694740:intron 20.0%
!! AAAAAAGCTGGTAAAATTAA+TGG - chr4_4:64093172-64093191 None:intergenic 20.0%
!! AAAGCATAAATAAAAAGTCT+AGG - chr4_4:64093794-64093813 None:intergenic 20.0%
!! TCATAAAAAATTAGCTAAAC+TGG - chr4_4:64093942-64093961 None:intergenic 20.0%
!! AATTATTGGTCTAGAAACAA+AGG - chr4_4:64091114-64091133 None:intergenic 25.0%
!!! TTCATTTTTCAATTCTTCTC+AGG + chr4_4:64091139-64091158 Msa0694740:CDS 25.0%
!! TAAAGAAGGTTCTTTTCTTA+AGG + chr4_4:64091272-64091291 Msa0694740:intron 25.0%
!! AAAGAAGGTTCTTTTCTTAA+GGG + chr4_4:64091273-64091292 Msa0694740:intron 25.0%
!!! AGCATCTCTATTTTATTTTC+AGG - chr4_4:64091377-64091396 None:intergenic 25.0%
! ATTAAGCATTTCACATTTCT+CGG - chr4_4:64091783-64091802 None:intergenic 25.0%
!! CCTTAAATGGTTAACAATTT+TGG - chr4_4:64091867-64091886 None:intergenic 25.0%
! CCAAAATTGTTAACCATTTA+AGG + chr4_4:64091864-64091883 Msa0694740:intron 25.0%
! ATATGTGTCATATCCTTAAA+TGG - chr4_4:64091880-64091899 None:intergenic 25.0%
!!! TTTTTTGTCAATAACAGTTC+AGG - chr4_4:64092030-64092049 None:intergenic 25.0%
!!! AATCAACCGAAATTTTGATA+CGG - chr4_4:64092150-64092169 None:intergenic 25.0%
! TGACAAAAAAATTAAGATGC+AGG - chr4_4:64092196-64092215 None:intergenic 25.0%
! GAAATATTGCAAAAGGATTT+AGG + chr4_4:64092257-64092276 Msa0694740:intron 25.0%
! TGATGTAAGATAAGAATGAT+GGG - chr4_4:64092301-64092320 None:intergenic 25.0%
! ATGATGTAAGATAAGAATGA+TGG - chr4_4:64092302-64092321 None:intergenic 25.0%
!!! TCATTATTTTACTACGAATC+TGG + chr4_4:64092404-64092423 Msa0694740:intron 25.0%
! TTAATAAGCATGATTAGACT+AGG - chr4_4:64092512-64092531 None:intergenic 25.0%
!!! AATAGAATTTAGCCTTTTTG+AGG + chr4_4:64092986-64093005 Msa0694740:intron 25.0%
! CTTAATACTTATCTTAGATG+AGG - chr4_4:64093114-64093133 None:intergenic 25.0%
! TTTGTTTCGATTAAAATAGG+TGG - chr4_4:64093843-64093862 None:intergenic 25.0%
! TAGCATATTCATTAATGATG+TGG + chr4_4:64093964-64093983 Msa0694740:intron 25.0%
! AATCACACAAATCTTGTAAA+AGG - chr4_4:64094800-64094819 None:intergenic 25.0%
!!! TTATTTGTCTAATGTTTTGG+TGG + chr4_4:64094844-64094863 Msa0694740:three_prime_UTR 25.0%
!!! AGAGAGAGTTTCTTCTTTTT+TGG + chr4_4:64090695-64090714 Msa0694740:intron 30.0%
TGTTATGAATCATGTTGTGT+TGG + chr4_4:64090886-64090905 Msa0694740:intron 30.0%
! AACAATAACTGACTTTACCT+CGG + chr4_4:64090961-64090980 Msa0694740:intron 30.0%
TGATACATAAATCTTCTGAC+AGG - chr4_4:64091013-64091032 None:intergenic 30.0%
TTATGAACATGTGAAAGAGA+AGG - chr4_4:64091069-64091088 None:intergenic 30.0%
TTGTTTAGACAACCAATCTA+AGG - chr4_4:64091205-64091224 None:intergenic 30.0%
TTTCTTGTTCTGCATAAAGA+AGG + chr4_4:64091258-64091277 Msa0694740:intron 30.0%
TGAATTCCTCATATATTTGG+AGG + chr4_4:64091551-64091570 Msa0694740:intron 30.0%
TTAAACTTACAGGAAGAAAC+AGG + chr4_4:64091644-64091663 Msa0694740:intron 30.0%
! GTTTTTACACATCACTCTAA+GGG + chr4_4:64091718-64091737 Msa0694740:CDS 30.0%
TGGAATGTTCATAACTCATA+AGG - chr4_4:64092119-64092138 None:intergenic 30.0%
CTTGTACCGTATCAAAATTT+CGG + chr4_4:64092141-64092160 Msa0694740:intron 30.0%
GTATCAAAATTTCGGTTGAT+TGG + chr4_4:64092149-64092168 Msa0694740:intron 30.0%
!! TGGCCTTTTAATTCATATCA+AGG + chr4_4:64092169-64092188 Msa0694740:intron 30.0%
GGACCTTGATATGAATTAAA+AGG - chr4_4:64092175-64092194 None:intergenic 30.0%
ATCATGCTTATTAACATAGC+AGG + chr4_4:64092518-64092537 Msa0694740:intron 30.0%
! AATATTTTCTCAATGCTGCT+TGG - chr4_4:64092636-64092655 None:intergenic 30.0%
! AGCAGCATTGAGAAAATATT+TGG + chr4_4:64092636-64092655 Msa0694740:CDS 30.0%
! GCAGCATTGAGAAAATATTT+GGG + chr4_4:64092637-64092656 Msa0694740:CDS 30.0%
! CAGCATTGAGAAAATATTTG+GGG + chr4_4:64092638-64092657 Msa0694740:CDS 30.0%
AACAACAAGTACTTGCAATT+AGG + chr4_4:64092673-64092692 Msa0694740:CDS 30.0%
GTTCTCCTTACAAACATATT+CGG + chr4_4:64093010-64093029 Msa0694740:intron 30.0%
TTCTCCTTACAAACATATTC+GGG + chr4_4:64093011-64093030 Msa0694740:intron 30.0%
AATTCCCGAATATGTTTGTA+AGG - chr4_4:64093018-64093037 None:intergenic 30.0%
! GGTAAAATTAATGGATCAAG+CGG - chr4_4:64093163-64093182 None:intergenic 30.0%
TTCATGATCGATAAAAAAGC+TGG - chr4_4:64093184-64093203 None:intergenic 30.0%
AGTATCCTAACTCTTATATG+TGG + chr4_4:64093209-64093228 Msa0694740:intron 30.0%
AAGTACCACATATAAGAGTT+AGG - chr4_4:64093217-64093236 None:intergenic 30.0%
ATGTGGTACTTGCAATATAT+AGG + chr4_4:64093226-64093245 Msa0694740:intron 30.0%
! TGGAATTTTTGTAAAGTCTG+AGG + chr4_4:64093627-64093646 Msa0694740:intron 30.0%
! TCTCCTTGTTTCACAAATTT+CGG + chr4_4:64093900-64093919 Msa0694740:intron 30.0%
AAATTAGCTAAACTGGATTG+TGG - chr4_4:64093935-64093954 None:intergenic 30.0%
GTTGACTATGTTAACAGTTA+TGG + chr4_4:64094042-64094061 Msa0694740:intron 30.0%
ACTATGTTAACAGTTATGGA+AGG + chr4_4:64094046-64094065 Msa0694740:intron 30.0%
!!! ATAGTCGATCTTGTTGATTT+TGG - chr4_4:64094302-64094321 None:intergenic 30.0%
AGTAATCCAAGAGAAAGATT+TGG + chr4_4:64094542-64094561 Msa0694740:CDS 30.0%
GGTCATTCAAATGGAATAAA+GGG + chr4_4:64094645-64094664 Msa0694740:CDS 30.0%
! GTCAATGTTCTCTAGAAATA+AGG + chr4_4:64094667-64094686 Msa0694740:CDS 30.0%
TCTAGAAATAAGGATTCTCA+TGG + chr4_4:64094677-64094696 Msa0694740:CDS 30.0%
! TATGATTGAGAGTGTATTAG+TGG + chr4_4:64094897-64094916 Msa0694740:three_prime_UTR 30.0%
!! GACAGTACTTTTTGCAACTT+TGG + chr4_4:64090735-64090754 Msa0694740:intron 35.0%
! ACAATAACTGACTTTACCTC+GGG + chr4_4:64090962-64090981 Msa0694740:intron 35.0%
ATTGAATTAAAAGGATCCCG+AGG - chr4_4:64090981-64091000 None:intergenic 35.0%
ACATAAATCTTCTGACAGGT+TGG - chr4_4:64091009-64091028 None:intergenic 35.0%
CACACAAAGAACAATCAACA+TGG - chr4_4:64091479-64091498 None:intergenic 35.0%
!! TTGGTTTTCAGCAATCAAGA+GGG + chr4_4:64091696-64091715 Msa0694740:CDS 35.0%
! GGTTTTTACACATCACTCTA+AGG + chr4_4:64091717-64091736 Msa0694740:CDS 35.0%
TCGGAATAGACAATGTAAGA+AGG - chr4_4:64091764-64091783 None:intergenic 35.0%
TTTGTATGTTGTTCTCGTTG+TGG + chr4_4:64091808-64091827 Msa0694740:intron 35.0%
CTCGTTGTGGTTCTTATAAT+TGG + chr4_4:64091821-64091840 Msa0694740:intron 35.0%
GATTCAAACCATGTTCTTGA+GGG - chr4_4:64091903-64091922 None:intergenic 35.0%
AGATTCAAACCATGTTCTTG+AGG - chr4_4:64091904-64091923 None:intergenic 35.0%
TGTAGCCAATTTGATGAGAA+TGG + chr4_4:64092052-64092071 Msa0694740:intron 35.0%
ACAGTCCATTCTCATCAAAT+TGG - chr4_4:64092060-64092079 None:intergenic 35.0%
!! TTGATGAGAATGGACTGTAA+TGG + chr4_4:64092062-64092081 Msa0694740:intron 35.0%
!! ATTTTGATACGGTACAAGCT+TGG - chr4_4:64092139-64092158 None:intergenic 35.0%
AGCACAAAAGAGAAGAAGAA+AGG + chr4_4:64092555-64092574 Msa0694740:CDS 35.0%
! AAAGAGAAGAAGAAAGGTGT+AGG + chr4_4:64092561-64092580 Msa0694740:CDS 35.0%
!!! AAGAGTTTTAGAGCTCTGAA+AGG + chr4_4:64093254-64093273 Msa0694740:CDS 35.0%
!!! TTTTAGAGCTCTGAAAGGTT+TGG + chr4_4:64093259-64093278 Msa0694740:CDS 35.0%
AATCCAGATGTTAGAAAACC+AGG + chr4_4:64093391-64093410 Msa0694740:CDS 35.0%
!! ATTTTTGTAAAGTCTGAGGC+AGG + chr4_4:64093631-64093650 Msa0694740:intron 35.0%
TGATAGCTTACTGACAAAAG+AGG + chr4_4:64093672-64093691 Msa0694740:CDS 35.0%
CATCAAAAGAGAGAGATCAA+TGG + chr4_4:64093732-64093751 Msa0694740:intron 35.0%
ATAAAAAGTCTAGGCTGTGT+TGG - chr4_4:64093785-64093804 None:intergenic 35.0%
! CTCCTTGTTTCACAAATTTC+GGG + chr4_4:64093901-64093920 Msa0694740:intron 35.0%
TTCCCGAAATTTGTGAAACA+AGG - chr4_4:64093906-64093925 None:intergenic 35.0%
ATCAAACCCACAAGAAAAAC+AGG + chr4_4:64094160-64094179 Msa0694740:CDS 35.0%
GTGTGAATTGGAAATTCTTC+AGG - chr4_4:64094187-64094206 None:intergenic 35.0%
! TTTTGTTCCGAATATGGTCT+TGG - chr4_4:64094212-64094231 None:intergenic 35.0%
! CTTGTCTTTTGTTCCGAATA+TGG - chr4_4:64094218-64094237 None:intergenic 35.0%
TCCATGTTATCAAAGGCAAA+AGG - chr4_4:64094269-64094288 None:intergenic 35.0%
TGGTGTATCCATGTTATCAA+AGG - chr4_4:64094276-64094295 None:intergenic 35.0%
!!! GATCTTGTTGATTTTGGAGT+TGG - chr4_4:64094296-64094315 None:intergenic 35.0%
!!! GTTCTAGATGGTTTTGAAGA+TGG - chr4_4:64094329-64094348 None:intergenic 35.0%
!! TTTCCTTGTGGAGTTCTAAA+TGG - chr4_4:64094356-64094375 None:intergenic 35.0%
TGTCAAAATATTCAAGGCCA+AGG + chr4_4:64094390-64094409 Msa0694740:CDS 35.0%
GTCAAAATATTCAAGGCCAA+GGG + chr4_4:64094391-64094410 Msa0694740:CDS 35.0%
TCAAAATATTCAAGGCCAAG+GGG + chr4_4:64094392-64094411 Msa0694740:CDS 35.0%
AGACTATCATCATCCTTCAA+CGG - chr4_4:64094446-64094465 None:intergenic 35.0%
TTTCCTTTGTCACAAGGAAT+AGG + chr4_4:64094623-64094642 Msa0694740:CDS 35.0%
TTCCTTTGTCACAAGGAATA+GGG + chr4_4:64094624-64094643 Msa0694740:CDS 35.0%
AAGGAATAGGGTCATTCAAA+TGG + chr4_4:64094636-64094655 Msa0694740:CDS 35.0%
GGGTCATTCAAATGGAATAA+AGG + chr4_4:64094644-64094663 Msa0694740:CDS 35.0%
TCAAGTGACTGGTATTTATC+TGG - chr4_4:64094716-64094735 None:intergenic 35.0%
TACCAGTCACTTGAATCTAT+TGG + chr4_4:64094722-64094741 Msa0694740:CDS 35.0%
TTCCAATAGATTCAAGTGAC+TGG - chr4_4:64094727-64094746 None:intergenic 35.0%
ATCTATTGGAAATGTGAGTG+TGG + chr4_4:64094736-64094755 Msa0694740:CDS 35.0%
AAGCTCTGTGACAACTAACA+AGG + chr4_4:64090758-64090777 Msa0694740:CDS 40.0%
! TGAATCATGTTGTGTTGGCA+TGG + chr4_4:64090891-64090910 Msa0694740:intron 40.0%
!! CATGTGATGGATCCTTAGAT+TGG + chr4_4:64091190-64091209 Msa0694740:intron 40.0%
CAGCTACCTCCAAATATATG+AGG - chr4_4:64091560-64091579 None:intergenic 40.0%
!!! GTTGGTTTTCAGCAATCAAG+AGG + chr4_4:64091695-64091714 Msa0694740:CDS 40.0%
! TACACATCACTCTAAGGGAA+AGG + chr4_4:64091723-64091742 Msa0694740:CDS 40.0%
AGACAATGTAAGAAGGCAGT+AGG - chr4_4:64091757-64091776 None:intergenic 40.0%
ACACATATCCCTCAAGAACA+TGG + chr4_4:64091892-64091911 Msa0694740:intron 40.0%
AAAGTCTCAGCTCAGAAAAC+AGG - chr4_4:64091973-64091992 None:intergenic 40.0%
AAACGAAGTTCAGGCAAAAG+AGG - chr4_4:64092358-64092377 None:intergenic 40.0%
! TTTTGCCTGAACTTCGTTTC+TGG + chr4_4:64092359-64092378 Msa0694740:intron 40.0%
GGTGTAGGAAAACTAAAGCA+TGG + chr4_4:64092576-64092595 Msa0694740:CDS 40.0%
GAATGTTCATCAACACAAGG+AGG + chr4_4:64092851-64092870 Msa0694740:CDS 40.0%
! TGGTTCTGTAAAGTTGGTTC+TGG - chr4_4:64092888-64092907 None:intergenic 40.0%
!! ACATGCTGGTTCTGTAAAGT+TGG - chr4_4:64092894-64092913 None:intergenic 40.0%
TTTACAGAACCAGCATGTCT+CGG + chr4_4:64092896-64092915 Msa0694740:CDS 40.0%
GTAAGGAGAACACCTCAAAA+AGG - chr4_4:64093001-64093020 None:intergenic 40.0%
GTACTTGCAATATATAGGCC+AGG + chr4_4:64093231-64093250 Msa0694740:intron 40.0%
! AAAGGTTTGGTAAGGCTTCA+AGG + chr4_4:64093272-64093291 Msa0694740:CDS 40.0%
TAAGAGGACAGAATGTGAAG+AGG + chr4_4:64093300-64093319 Msa0694740:CDS 40.0%
TAACAGCTGCATGTGTTTCA+TGG - chr4_4:64093339-64093358 None:intergenic 40.0%
TCCAGCTTCATTCTTGTACT+CGG - chr4_4:64093429-64093448 None:intergenic 40.0%
!! AATGAAGCTGGAAGTACCTT+GGG + chr4_4:64093437-64093456 Msa0694740:CDS 40.0%
AGGCAGGTAACAATGAAGAT+TGG + chr4_4:64093647-64093666 Msa0694740:CDS 40.0%
GGCAGGTAACAATGAAGATT+GGG + chr4_4:64093648-64093667 Msa0694740:CDS 40.0%
GGCATTTGCATATTCACATC+AGG + chr4_4:64093753-64093772 Msa0694740:intron 40.0%
!!! CGTTTGAGTTGATTTTGGAG+TGG - chr4_4:64094072-64094091 None:intergenic 40.0%
!!! ACAGGCGTTTGAGTTGATTT+TGG - chr4_4:64094077-64094096 None:intergenic 40.0%
! TTTTCTTGTGGGTTTGATGC+AGG - chr4_4:64094158-64094177 None:intergenic 40.0%
!! TCAGGACCTGTTTTTCTTGT+GGG - chr4_4:64094169-64094188 None:intergenic 40.0%
!! TTCAGGACCTGTTTTTCTTG+TGG - chr4_4:64094170-64094189 None:intergenic 40.0%
!! GCCTTTTGCCTTTGATAACA+TGG + chr4_4:64094265-64094284 Msa0694740:CDS 40.0%
!! CTAAATGGAGGAGTTCTAGA+TGG - chr4_4:64094341-64094360 None:intergenic 40.0%
GCGCGATGTCAAAATATTCA+AGG + chr4_4:64094384-64094403 Msa0694740:CDS 40.0%
! ATGTAGTTTGGAACGGAGAA+CGG - chr4_4:64094482-64094501 None:intergenic 40.0%
ATTACTACTAGCGCGTACTT+TGG - chr4_4:64094528-64094547 None:intergenic 40.0%
GTCTCATTTCCTTTGTCACA+AGG + chr4_4:64094617-64094636 Msa0694740:CDS 40.0%
! ATGTGGTGCTGGAAAGAAGA+AGG + chr4_4:64090634-64090653 Msa0694740:intron 45.0%
AAACAGGAATGCTGCTGAGA+TGG + chr4_4:64091660-64091679 Msa0694740:CDS 45.0%
AACAGGAATGCTGCTGAGAT+GGG + chr4_4:64091661-64091680 Msa0694740:CDS 45.0%
TCCAATCACATTGAGCGTCT+CGG + chr4_4:64091994-64092013 Msa0694740:intron 45.0%
TGCTTCCAGAAACGAAGTTC+AGG - chr4_4:64092367-64092386 None:intergenic 45.0%
GAAGAGGGGGATGAAAGAAT+TGG - chr4_4:64092607-64092626 None:intergenic 45.0%
! TAGGAGAAGCAACTCTAGGA+GGG - chr4_4:64092740-64092759 None:intergenic 45.0%
GCGGAATGTTCATCAACACA+AGG + chr4_4:64092848-64092867 Msa0694740:CDS 45.0%
TATAACCTCTGTAAGCAGCC+TGG - chr4_4:64092932-64092951 None:intergenic 45.0%
GGCTGCTTACAGAGGTTATA+TGG + chr4_4:64092932-64092951 Msa0694740:CDS 45.0%
!! CAGAGCTCTAAAACTCTTCC+TGG - chr4_4:64093252-64093271 None:intergenic 45.0%
!! GAGCTCTGAAAGGTTTGGTA+AGG + chr4_4:64093264-64093283 Msa0694740:CDS 45.0%
TTTGGTAAGGCTTCAAGGAG+TGG + chr4_4:64093277-64093296 Msa0694740:CDS 45.0%
!! GTGCCTGGTTTTCTAACATC+TGG - chr4_4:64093397-64093416 None:intergenic 45.0%
!! GAATGAAGCTGGAAGTACCT+TGG + chr4_4:64093436-64093455 Msa0694740:CDS 45.0%
!! ATGAAGCTGGAAGTACCTTG+GGG + chr4_4:64093438-64093457 Msa0694740:CDS 45.0%
!! ACCTTGGGGAAAAGTGCTTT+AGG + chr4_4:64093452-64093471 Msa0694740:CDS 45.0%
!! TCCTAAAGCACTTTTCCCCA+AGG - chr4_4:64093456-64093475 None:intergenic 45.0%
!! GGGAAAAGTGCTTTAGGACA+TGG + chr4_4:64093458-64093477 Msa0694740:CDS 45.0%
CAAAAGAGGAAGTAGAAGCG+AGG + chr4_4:64093686-64093705 Msa0694740:CDS 45.0%
! AAAGTCTAGGCTGTGTTGGT+GGG - chr4_4:64093781-64093800 None:intergenic 45.0%
AAAAGTCTAGGCTGTGTTGG+TGG - chr4_4:64093782-64093801 None:intergenic 45.0%
!! ATGGTCTTGGTGGTGTGAAT+TGG - chr4_4:64094199-64094218 None:intergenic 45.0%
CACACCACCAAGACCATATT+CGG + chr4_4:64094202-64094221 Msa0694740:CDS 45.0%
! TGTTCCGAATATGGTCTTGG+TGG - chr4_4:64094209-64094228 None:intergenic 45.0%
CAAAAGACAAGTCCAAGACC+TGG + chr4_4:64094227-64094246 Msa0694740:CDS 45.0%
!! CCTTGTGGAGTTCTAAATGG+AGG - chr4_4:64094353-64094372 None:intergenic 45.0%
! CCTCCATTTAGAACTCCACA+AGG + chr4_4:64094350-64094369 Msa0694740:CDS 45.0%
CGGTACATCGAAAGGTGAAT+TGG - chr4_4:64094426-64094445 None:intergenic 45.0%
ACCTTTCGATGTACCGTTGA+AGG + chr4_4:64094430-64094449 Msa0694740:CDS 45.0%
TCCTTCAACGGTACATCGAA+AGG - chr4_4:64094434-64094453 None:intergenic 45.0%
GTTCTCCGTTCCAAACTACA+TGG + chr4_4:64094481-64094500 Msa0694740:CDS 45.0%
TGGAGCCATGTAGTTTGGAA+CGG - chr4_4:64094489-64094508 None:intergenic 45.0%
ACAGTTGGAGCCATGTAGTT+TGG - chr4_4:64094494-64094513 None:intergenic 45.0%
CCACAGCCAAATCTTTCTCT+TGG - chr4_4:64094551-64094570 None:intergenic 45.0%
CCAAGAGAAAGATTTGGCTG+TGG + chr4_4:64094548-64094567 Msa0694740:CDS 45.0%
AGATTTGGCTGTGGTAGTAG+TGG + chr4_4:64094557-64094576 Msa0694740:CDS 45.0%
CTCTTGGAATCAGTGCTTGT+TGG - chr4_4:64094593-64094612 None:intergenic 45.0%
!! CAACAAGCACTGATTCCAAG+AGG + chr4_4:64094591-64094610 Msa0694740:CDS 45.0%
AGGAAATGAGACTCTCCTCT+TGG - chr4_4:64094609-64094628 None:intergenic 45.0%
GACCCTATTCCTTGTGACAA+AGG - chr4_4:64094629-64094648 None:intergenic 45.0%
!!! TGTTTTTTTAATTATTATTA+TGG + chr4_4:64090860-64090879 Msa0694740:intron 5.0%
!!! TTATTTATTCAATTAATTTT+TGG + chr4_4:64091341-64091360 Msa0694740:intron 5.0%
!!! TATAAAAAATAAAAGTATTT+AGG + chr4_4:64093054-64093073 Msa0694740:intron 5.0%
!! TAAAAAAAAAAAAACATAAT+AGG + chr4_4:64094960-64094979 Msa0694740:three_prime_UTR 5.0%
! TGGTGCTGGAAAGAAGAAGG+AGG + chr4_4:64090637-64090656 Msa0694740:intron 50.0%
TGCTGCTGAGATGGGAAAGA+AGG + chr4_4:64091669-64091688 Msa0694740:CDS 50.0%
GCTGCTGAGATGGGAAAGAA+GGG + chr4_4:64091670-64091689 Msa0694740:CDS 50.0%
CTGCTGAGATGGGAAAGAAG+GGG + chr4_4:64091671-64091690 Msa0694740:CDS 50.0%
AGATGGGAAAGAAGGGGAGT+TGG + chr4_4:64091677-64091696 Msa0694740:CDS 50.0%
TCCGAGACGCTCAATGTGAT+TGG - chr4_4:64091998-64092017 None:intergenic 50.0%
! TGCTTGGTTCTCTGAAGAGG+GGG - chr4_4:64092620-64092639 None:intergenic 50.0%
! CTGCTTGGTTCTCTGAAGAG+GGG - chr4_4:64092621-64092640 None:intergenic 50.0%
! GCTGCTTGGTTCTCTGAAGA+GGG - chr4_4:64092622-64092641 None:intergenic 50.0%
! TGCTGCTTGGTTCTCTGAAG+AGG - chr4_4:64092623-64092642 None:intergenic 50.0%
CTAGGAGAAGCAACTCTAGG+AGG - chr4_4:64092741-64092760 None:intergenic 50.0%
CTCCTAGAGTTGCTTCTCCT+AGG + chr4_4:64092739-64092758 Msa0694740:CDS 50.0%
TCCTAAGCCTCCTTCTCCAA+GGG + chr4_4:64092770-64092789 Msa0694740:CDS 50.0%
TCCAAGGGATCCATCTCCAA+GGG + chr4_4:64092785-64092804 Msa0694740:CDS 50.0%
AAGATCCAGGCTGCTTACAG+AGG + chr4_4:64092924-64092943 Msa0694740:CDS 50.0%
AGGCTTCAAGGAGTGGTAAG+AGG + chr4_4:64093284-64093303 Msa0694740:CDS 50.0%
GCCGAGTACAAGAATGAAGC+TGG + chr4_4:64093425-64093444 Msa0694740:CDS 50.0%
AAGTAGAAGCGAGGTTGCAG+AGG + chr4_4:64093695-64093714 Msa0694740:CDS 50.0%
! GCGCTTGAAGTGTTTCCTTG+TGG - chr4_4:64094368-64094387 None:intergenic 50.0%
TATTCAAGGCCAAGGGGAGT+AGG + chr4_4:64094398-64094417 Msa0694740:CDS 50.0%
GAATTGGATCCTACTCCCCT+TGG - chr4_4:64094410-64094429 None:intergenic 50.0%
! TAGTTTGGAACGGAGAACGG+TGG - chr4_4:64094479-64094498 None:intergenic 50.0%
!! TTGGCCTTGGCAGAAACAGT+TGG - chr4_4:64094509-64094528 None:intergenic 50.0%
AGGATTCTCATGGCTCTCAC+AGG + chr4_4:64094687-64094706 Msa0694740:CDS 50.0%
AGCAGTATCCTTGCCTGCAA+GGG + chr4_4:64094763-64094782 Msa0694740:CDS 50.0%
TCTTTCAACCCTTGCAGGCA+AGG - chr4_4:64094774-64094793 None:intergenic 50.0%
GGCTTTCTTTCAACCCTTGC+AGG - chr4_4:64094779-64094798 None:intergenic 50.0%
CAGAACGATCGACAGCAAGG+AGG - chr4_4:64091401-64091420 None:intergenic 55.0%
! CTCCTTGCTGTCGATCGTTC+TGG + chr4_4:64091399-64091418 Msa0694740:intron 55.0%
CACCAGAACGATCGACAGCA+AGG - chr4_4:64091404-64091423 None:intergenic 55.0%
!!! AGGTGGCGTTTTTGGTCGCT+CGG - chr4_4:64092712-64092731 None:intergenic 55.0%
! GGGACGAAAGGTGGCGTTTT+TGG - chr4_4:64092720-64092739 None:intergenic 55.0%
! ACTCTAGGAGGGACGAAAGG+TGG - chr4_4:64092729-64092748 None:intergenic 55.0%
! GCAACTCTAGGAGGGACGAA+AGG - chr4_4:64092732-64092751 None:intergenic 55.0%
GGCCTAGGAGAAGCAACTCT+AGG - chr4_4:64092744-64092763 None:intergenic 55.0%
GAAGGAGGCTTAGGAGAAGG+AGG - chr4_4:64092765-64092784 None:intergenic 55.0%
GGAGAAGGAGGCTTAGGAGA+AGG - chr4_4:64092768-64092787 None:intergenic 55.0%
CTCCTAAGCCTCCTTCTCCA+AGG + chr4_4:64092769-64092788 Msa0694740:CDS 55.0%
TCCCTTGGAGAAGGAGGCTT+AGG - chr4_4:64092774-64092793 None:intergenic 55.0%
GATGGATCCCTTGGAGAAGG+AGG - chr4_4:64092780-64092799 None:intergenic 55.0%
GGAGATGGATCCCTTGGAGA+AGG - chr4_4:64092783-64092802 None:intergenic 55.0%
CTCCAAGGGATCCATCTCCA+AGG + chr4_4:64092784-64092803 Msa0694740:CDS 55.0%
AGAGGTAACTCGCGGAGAAG+CGG - chr4_4:64092811-64092830 None:intergenic 55.0%
GCTTTCGGAGAGGTAACTCG+CGG - chr4_4:64092819-64092838 None:intergenic 55.0%
GCGAGTTACCTCTCCGAAAG+CGG + chr4_4:64092818-64092837 Msa0694740:CDS 55.0%
!! TTCCGCGAAGATGCCGCTTT+CGG - chr4_4:64092834-64092853 None:intergenic 55.0%
TCTTAGTCGCCGAGACATGC+TGG - chr4_4:64092908-64092927 None:intergenic 55.0%
TGTCTCGGCGACTAAGATCC+AGG + chr4_4:64092911-64092930 Msa0694740:CDS 55.0%
! ACTCGGCTTGATATCGTGCC+TGG - chr4_4:64093412-64093431 None:intergenic 55.0%
AGAAGCGAGGTTGCAGAGGA+AGG + chr4_4:64093699-64093718 Msa0694740:CDS 55.0%
CTCGATCGCGTATCTGTCAC+AGG - chr4_4:64094095-64094114 None:intergenic 55.0%
GTGACAGATACGCGATCGAG+CGG + chr4_4:64094095-64094114 Msa0694740:CDS 55.0%
!! GATTCCCATGGTGGTGGAAC+TGG + chr4_4:64094117-64094136 Msa0694740:CDS 55.0%
CCAACCAGTTCCACCACCAT+GGG - chr4_4:64094124-64094143 None:intergenic 55.0%
TCCAACCAGTTCCACCACCA+TGG - chr4_4:64094125-64094144 None:intergenic 55.0%
TGAGAGCTTGAGCCAGGTCT+TGG - chr4_4:64094242-64094261 None:intergenic 55.0%
GACCTGGCTCAAGCTCTCAA+AGG + chr4_4:64094243-64094262 Msa0694740:CDS 55.0%
!! GGCTCCAACTGTTTCTGCCA+AGG + chr4_4:64094502-64094521 Msa0694740:CDS 55.0%
ACTAGCGCGTACTTTGGCCT+TGG - chr4_4:64094522-64094541 None:intergenic 55.0%
CAGCAGTATCCTTGCCTGCA+AGG + chr4_4:64094762-64094781 Msa0694740:CDS 55.0%
! TTTGGTCGCTCGGGAGGTGT+GGG - chr4_4:64092702-64092721 None:intergenic 60.0%
! TTTTGGTCGCTCGGGAGGTG+TGG - chr4_4:64092703-64092722 None:intergenic 60.0%
!!! GGTGGCGTTTTTGGTCGCTC+GGG - chr4_4:64092711-64092730 None:intergenic 60.0%
GGCTTAGGAGAAGGAGGCCT+AGG - chr4_4:64092759-64092778 None:intergenic 60.0%
GCCCTTGGAGATGGATCCCT+TGG - chr4_4:64092789-64092808 None:intergenic 60.0%
!! CGAAGATGCCGCTTTCGGAG+AGG - chr4_4:64092829-64092848 None:intergenic 60.0%
CTCCGAAAGCGGCATCTTCG+CGG + chr4_4:64092829-64092848 Msa0694740:CDS 60.0%
! GATCGAGCGGATTCCCATGG+TGG + chr4_4:64094108-64094127 Msa0694740:CDS 60.0%
!! CCCATGGTGGTGGAACTGGT+TGG + chr4_4:64094121-64094140 Msa0694740:CDS 60.0%
! GGCCTTTGAGAGCTTGAGCC+AGG - chr4_4:64094248-64094267 None:intergenic 60.0%
!!! GGCGTTTTTGGTCGCTCGGG+AGG - chr4_4:64092708-64092727 None:intergenic 65.0%
GGAGAAGCGGCCCTTGGAGA+TGG - chr4_4:64092798-64092817 None:intergenic 65.0%
! CGCGATCGAGCGGATTCCCA+TGG + chr4_4:64094105-64094124 Msa0694740:CDS 65.0%
! CGAGCGGATTCCCATGGTGG+TGG + chr4_4:64094111-64094130 Msa0694740:CDS 65.0%
ACTCGCGGAGAAGCGGCCCT+TGG - chr4_4:64092804-64092823 None:intergenic 70.0%
! GGTCGCTCGGGAGGTGTGGG+AGG - chr4_4:64092699-64092718 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 64090634 64095044 64090634 ID=Msa0694740;Name=Msa0694740
chr4_4 mRNA 64090634 64095044 64090634 ID=Msa0694740-mRNA-1;Parent=Msa0694740;Name=Msa0694740-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0.42|0.5|1|0.85|0.87|8|227|551
chr4_4 exon 64090634 64090638 64090634 ID=Msa0694740-mRNA-1:exon:18285;Parent=Msa0694740-mRNA-1
chr4_4 exon 64090740 64090779 64090740 ID=Msa0694740-mRNA-1:exon:18286;Parent=Msa0694740-mRNA-1
chr4_4 exon 64091124 64091160 64091124 ID=Msa0694740-mRNA-1:exon:18287;Parent=Msa0694740-mRNA-1
chr4_4 exon 64091656 64091744 64091656 ID=Msa0694740-mRNA-1:exon:18288;Parent=Msa0694740-mRNA-1
chr4_4 exon 64092540 64092953 64092540 ID=Msa0694740-mRNA-1:exon:18289;Parent=Msa0694740-mRNA-1
chr4_4 exon 64093248 64093481 64093248 ID=Msa0694740-mRNA-1:exon:18290;Parent=Msa0694740-mRNA-1
chr4_4 exon 64093643 64093720 64093643 ID=Msa0694740-mRNA-1:exon:18291;Parent=Msa0694740-mRNA-1
chr4_4 exon 64094059 64095044 64094059 ID=Msa0694740-mRNA-1:exon:18292;Parent=Msa0694740-mRNA-1
chr4_4 CDS 64090634 64090638 64090634 ID=Msa0694740-mRNA-1:cds;Parent=Msa0694740-mRNA-1
chr4_4 CDS 64090740 64090779 64090740 ID=Msa0694740-mRNA-1:cds;Parent=Msa0694740-mRNA-1
chr4_4 CDS 64091124 64091160 64091124 ID=Msa0694740-mRNA-1:cds;Parent=Msa0694740-mRNA-1
chr4_4 CDS 64091656 64091744 64091656 ID=Msa0694740-mRNA-1:cds;Parent=Msa0694740-mRNA-1
chr4_4 CDS 64092540 64092953 64092540 ID=Msa0694740-mRNA-1:cds;Parent=Msa0694740-mRNA-1
chr4_4 CDS 64093248 64093481 64093248 ID=Msa0694740-mRNA-1:cds;Parent=Msa0694740-mRNA-1
chr4_4 CDS 64093643 64093720 64093643 ID=Msa0694740-mRNA-1:cds;Parent=Msa0694740-mRNA-1
chr4_4 CDS 64094059 64094817 64094059 ID=Msa0694740-mRNA-1:cds;Parent=Msa0694740-mRNA-1
chr4_4 three_prime_UTR 64094818 64095044 64094818 ID=Msa0694740-mRNA-1:three_prime_utr;Parent=Msa0694740-mRNA-1
Gene Sequence

>Msa0694740

ATGTGTACTTTTTGCAACTTTGGAAGCTCTGTGACAACTAACAAGACCAATAATTCATCTTTCATTTTTCAATTCTTCTCAGGAAGAAACAGGAATGCTGCTGAGATGGGAAAGAAGGGGAGTTGGTTTTCAGCAATCAAGAGGGTTTTTACACATCACTCTAAGGGAAAGGATTCAGAAAACAAAAGCACAAAAGAGAAGAAGAAAGGTGTAGGAAAACTAAAGCATGGAGAGACCAATTCTTTCATCCCCCTCTTCAGAGAACCAAGCAGCATTGAGAAAATATTTGGGGACTTTGAAAGAGAACAACAAGTACTTGCAATTAGGCCTCCCACACCTCCCGAGCGACCAAAAACGCCACCTTTCGTCCCTCCTAGAGTTGCTTCTCCTAGGCCTCCTTCTCCTAAGCCTCCTTCTCCAAGGGATCCATCTCCAAGGGCCGCTTCTCCGCGAGTTACCTCTCCGAAAGCGGCATCTTCGCGGAATGTTCATCAACACAAGGAGGTTCGCAACAGACCAGAACCAACTTTACAGAACCAGCATGTCTCGGCGACTAAGATCCAGGCTGCTTACAGAGGTTATATGGCCAGGAAGAGTTTTAGAGCTCTGAAAGGTTTGGTAAGGCTTCAAGGAGTGGTAAGAGGACAGAATGTGAAGAGGCAGACAGTTAATGCCATGAAACACATGCAGCTGTTAGTGCGAGTTCAGTCTCAGATTCAATCTCGAAGAATCCAGATGTTAGAAAACCAGGCACGATATCAAGCCGAGTACAAGAATGAAGCTGGAAGTACCTTGGGGAAAAGTGCTTTAGGACATGGTTCTGAGGCAGGTAACAATGAAGATTGGGATGATAGCTTACTGACAAAAGAGGAAGTAGAAGCGAGGTTGCAGAGGAAGTTATGGAAGGCCACTCCAAAATCAACTCAAACGCCTGTGACAGATACGCGATCGAGCGGATTCCCATGGTGGTGGAACTGGTTGGAAAGACAATTACCTGCATCAAACCCACAAGAAAAACAGGTCCTGAAGAATTTCCAATTCACACCACCAAGACCATATTCGGAACAAAAGACAAGTCCAAGACCTGGCTCAAGCTCTCAAAGGCCTTTTGCCTTTGATAACATGGATACACCAACTCCAAAATCAACAAGATCGACTATATTTCCATCTTCAAAACCATCTAGAACTCCTCCATTTAGAACTCCACAAGGAAACACTTCAAGCGCGATGTCAAAATATTCAAGGCCAAGGGGAGTAGGATCCAATTCACCTTTCGATGTACCGTTGAAGGATGATGATAGTCTCACAAGCTGTCCACCGTTCTCCGTTCCAAACTACATGGCTCCAACTGTTTCTGCCAAGGCCAAAGTACGCGCTAGTAGTAATCCAAGAGAAAGATTTGGCTGTGGTAGTAGTGGCTGTGCAACTCCAACAAGCACTGATTCCAAGAGGAGAGTCTCATTTCCTTTGTCACAAGGAATAGGGTCATTCAAATGGAATAAAGGGTCAATGTTCTCTAGAAATAAGGATTCTCATGGCTCTCACAGGACTCCAGATAAATACCAGTCACTTGAATCTATTGGAAATGTGAGTGTGGATTCAGCAGTATCCTTGCCTGCAAGGGTTGAAAGAAAGCCTTTTACAAGATTTGTGTGA
Protein sequence

>Msa0694740

MCTFCNFGSSVTTNKTNNSSFIFQFFSGRNRNAAEMGKKGSWFSAIKRVFTHHSKGKDSENKSTKEKKKGVGKLKHGETNSFIPLFREPSSIEKIFGDFEREQQVLAIRPPTPPERPKTPPFVPPRVASPRPPSPKPPSPRDPSPRAASPRVTSPKAASSRNVHQHKEVRNRPEPTLQNQHVSATKIQAAYRGYMARKSFRALKGLVRLQGVVRGQNVKRQTVNAMKHMQLLVRVQSQIQSRRIQMLENQARYQAEYKNEAGSTLGKSALGHGSEAGNNEDWDDSLLTKEEVEARLQRKLWKATPKSTQTPVTDTRSSGFPWWWNWLERQLPASNPQEKQVLKNFQFTPPRPYSEQKTSPRPGSSSQRPFAFDNMDTPTPKSTRSTIFPSSKPSRTPPFRTPQGNTSSAMSKYSRPRGVGSNSPFDVPLKDDDSLTSCPPFSVPNYMAPTVSAKAKVRASSNPRERFGCGSSGCATPTSTDSKRRVSFPLSQGIGSFKWNKGSMFSRNKDSHGSHRTPDKYQSLESIGNVSVDSAVSLPARVERKPFTRFV*