AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0702100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0702100 MtrunA17_Chr8g0380411 98.374 123 2 0 3 125 5 127 2.98e-84 244
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0702100 AT3G29130.1 64.103 117 42 0 9 125 3 119 4.01e-55 168
Msa0702100 AT3G29130.6 64.103 117 42 0 9 125 3 119 4.01e-55 168
Msa0702100 AT3G29130.5 64.103 117 42 0 9 125 3 119 4.01e-55 168
Msa0702100 AT3G29130.2 64.103 117 42 0 9 125 52 168 1.05e-54 169
Msa0702100 AT3G29130.4 65.000 80 28 0 46 125 12 91 9.97e-37 120
Msa0702100 AT3G29130.3 65.000 80 28 0 46 125 12 91 9.97e-37 120

Find 33 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATACATAGTGTGTTGTAAA+TGG 0.303412 4_4:+73601436 None:intergenic
TGGATAAGTTGTTGAAAGTT+TGG 0.311208 4_4:+73599622 None:intergenic
AATAGAAGACATGTTATTTC+AGG 0.314826 4_4:-73599283 Msa0702100:three_prime_UTR
GAATCAGAATCGATGAAATT+AGG 0.315765 4_4:-73601522 Msa0702100:CDS
CTTGAAATGCCTAAGTTATA+TGG 0.324858 4_4:-73599477 Msa0702100:three_prime_UTR
GAAGTTCAGGGGCAAGAAAT+TGG 0.328764 4_4:+73599333 None:intergenic
ACTTGTTAGCAATGAAGTTC+AGG 0.339437 4_4:+73599320 None:intergenic
TAGTGTGTTGTAAATGGTTA+AGG 0.342554 4_4:+73601442 None:intergenic
TTTCAGATTGGGAAGGTTGC+AGG 0.354404 4_4:-73601319 Msa0702100:intron
TACTTAGGCATTTCAAGATC+CGG 0.393355 4_4:+73599554 None:intergenic
TAGCCATGTCAGGAGAAATC+TGG 0.395052 4_4:+73601244 None:intergenic
TTCTTCATTTCTTGTCCAAA+AGG 0.409409 4_4:-73599387 Msa0702100:three_prime_UTR
AACTTAGGCATTTCAAGATC+CGG 0.422372 4_4:+73599483 None:intergenic
ACGTTACTCATAGAATCTTC+AGG 0.435689 4_4:+73599590 None:intergenic
GAATCTTCAGGAAATGCATC+TGG 0.446985 4_4:+73599602 None:intergenic
GTTATTCGAACGTGTTTGAC+AGG 0.458228 4_4:-73599509 Msa0702100:three_prime_UTR
GATAACAGCAATAGTTACTT+AGG 0.475331 4_4:+73599539 None:intergenic
TCAATAATTCATAATAACTA+AGG 0.478803 4_4:+73599430 None:intergenic
AGTGTGTTGTAAATGGTTAA+GGG 0.496258 4_4:+73601443 None:intergenic
CGTGTGTTTCTAGCCATGTC+AGG 0.515825 4_4:+73601234 None:intergenic
CTTGTTAGCAATGAAGTTCA+GGG 0.517721 4_4:+73599321 None:intergenic
GAACGTGTTTGACAGGAGAC+CGG 0.536487 4_4:-73599502 Msa0702100:three_prime_UTR
CTATGAGTAACGTTATTGAC+AGG 0.539299 4_4:-73599580 Msa0702100:CDS
TACCCAGATTTCTCCTGACA+TGG 0.542515 4_4:-73601247 Msa0702100:CDS
TAAACACGTCCATATAACTT+AGG 0.553646 4_4:+73599468 None:intergenic
AGCCATGTCAGGAGAAATCT+GGG 0.559247 4_4:+73601245 None:intergenic
AGAAATGAAGAAAACAATGC+AGG 0.559601 4_4:+73599398 None:intergenic
AAAACGGTTTCATTTGGTCA+CGG 0.573588 4_4:-73601571 Msa0702100:five_prime_UTR
AGAAGACATGTTATTTCAGG+AGG 0.574015 4_4:-73599280 Msa0702100:three_prime_UTR
TAACGTTATTGACAGGAGAC+CGG 0.586364 4_4:-73599573 Msa0702100:CDS
AATTGAATTTCAGATTGGGA+AGG 0.586674 4_4:-73601326 Msa0702100:intron
GTTAAGGGAACGAAGATCAT+CGG 0.608518 4_4:+73601458 None:intergenic
TTGTTAGCAATGAAGTTCAG+GGG 0.652292 4_4:+73599322 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATATTTTTCTTAAACTT+AGG - chr4_4:73599665-73599684 Msa0702100:intron 10.0%
!! ATATCATCAAATTATATAAT+TGG - chr4_4:73599729-73599748 Msa0702100:intron 10.0%
!!! TTATTGAAAATTCTTAATTT+TGG - chr4_4:73600159-73600178 Msa0702100:intron 10.0%
!!! ATTATATTTTTCTTAAACTT+AGG - chr4_4:73599665-73599684 Msa0702100:intron 10.0%
!! ATATCATCAAATTATATAAT+TGG - chr4_4:73599729-73599748 Msa0702100:intron 10.0%
!!! TTATTGAAAATTCTTAATTT+TGG - chr4_4:73600159-73600178 Msa0702100:intron 10.0%
!!! AGTTTGTATGTATATTTTTT+AGG - chr4_4:73599755-73599774 Msa0702100:intron 15.0%
!! TTTACAATTAGATAATAACA+AGG - chr4_4:73600752-73600771 Msa0702100:intron 15.0%
!! TCAATAATTCATAATAACTA+AGG + chr4_4:73601420-73601439 None:intergenic 15.0%
!!! AGTTTGTATGTATATTTTTT+AGG - chr4_4:73599755-73599774 Msa0702100:intron 15.0%
!! TTTACAATTAGATAATAACA+AGG - chr4_4:73600752-73600771 Msa0702100:intron 15.0%
!! TCAATAATTCATAATAACTA+AGG + chr4_4:73601420-73601439 None:intergenic 15.0%
!! AATTGAATTGAATTTCAGAT+TGG - chr4_4:73599516-73599535 Msa0702100:three_prime_UTR 20.0%
!! ATTGAATTGAATTTCAGATT+GGG - chr4_4:73599517-73599536 Msa0702100:three_prime_UTR 20.0%
!!! TGGAAATTGATAATAATCTT+GGG - chr4_4:73599825-73599844 Msa0702100:intron 20.0%
!! AAAATAGTATGATTCAGTTA+TGG - chr4_4:73600050-73600069 Msa0702100:intron 20.0%
!!! AATTTTCAATAAACAGAATC+AGG + chr4_4:73600151-73600170 None:intergenic 20.0%
!!! GTTTAGACTAAATATAGTTT+TGG - chr4_4:73600397-73600416 Msa0702100:intron 20.0%
!!! CTAACAAAATTTTGAGAAAA+TGG - chr4_4:73600452-73600471 Msa0702100:intron 20.0%
!!! TTAATCTTCAACATTTTACT+TGG + chr4_4:73600612-73600631 None:intergenic 20.0%
!! AATTGAATTGAATTTCAGAT+TGG - chr4_4:73599516-73599535 Msa0702100:three_prime_UTR 20.0%
!! ATTGAATTGAATTTCAGATT+GGG - chr4_4:73599517-73599536 Msa0702100:three_prime_UTR 20.0%
!!! TGGAAATTGATAATAATCTT+GGG - chr4_4:73599825-73599844 Msa0702100:intron 20.0%
!! AAAATAGTATGATTCAGTTA+TGG - chr4_4:73600050-73600069 Msa0702100:intron 20.0%
!!! AATTTTCAATAAACAGAATC+AGG + chr4_4:73600151-73600170 None:intergenic 20.0%
!!! GTTTAGACTAAATATAGTTT+TGG - chr4_4:73600397-73600416 Msa0702100:intron 20.0%
!!! CTAACAAAATTTTGAGAAAA+TGG - chr4_4:73600452-73600471 Msa0702100:intron 20.0%
!!! TTAATCTTCAACATTTTACT+TGG + chr4_4:73600612-73600631 None:intergenic 20.0%
! AAAAAAAAGAGAGATGAAAG+AGG + chr4_4:73599448-73599467 None:intergenic 25.0%
! AAAATCAAAGAAGCAATACA+AGG - chr4_4:73599802-73599821 Msa0702100:intron 25.0%
!! GTGGAAATTGATAATAATCT+TGG - chr4_4:73599824-73599843 Msa0702100:intron 25.0%
!! GGAAATTGATAATAATCTTG+GGG - chr4_4:73599826-73599845 Msa0702100:intron 25.0%
!! GAAATTGATAATAATCTTGG+GGG - chr4_4:73599827-73599846 Msa0702100:intron 25.0%
!! AAATCCGAATTTTATTCTCT+CGG - chr4_4:73599987-73600006 Msa0702100:intron 25.0%
!! TTTTCCGAGAGAATAAAATT+CGG + chr4_4:73599994-73600013 None:intergenic 25.0%
!! TAAACAGAATCAGGTTATTT+TGG + chr4_4:73600142-73600161 None:intergenic 25.0%
! CATCTATATCATCTAACATT+TGG - chr4_4:73600217-73600236 Msa0702100:intron 25.0%
!! TTTTTCTAATGTAACCTAGA+CGG - chr4_4:73600954-73600973 Msa0702100:intron 25.0%
! CTAACATAGATAGAAATAGA+AGG + chr4_4:73601122-73601141 None:intergenic 25.0%
!!! AATAGAAGACATGTTATTTC+AGG - chr4_4:73601564-73601583 Msa0702100:five_prime_UTR 25.0%
! AAAAAAAAGAGAGATGAAAG+AGG + chr4_4:73599448-73599467 None:intergenic 25.0%
! AAAATCAAAGAAGCAATACA+AGG - chr4_4:73599802-73599821 Msa0702100:intron 25.0%
!! GTGGAAATTGATAATAATCT+TGG - chr4_4:73599824-73599843 Msa0702100:intron 25.0%
!! GGAAATTGATAATAATCTTG+GGG - chr4_4:73599826-73599845 Msa0702100:intron 25.0%
!! GAAATTGATAATAATCTTGG+GGG - chr4_4:73599827-73599846 Msa0702100:intron 25.0%
!! AAATCCGAATTTTATTCTCT+CGG - chr4_4:73599987-73600006 Msa0702100:intron 25.0%
!! TTTTCCGAGAGAATAAAATT+CGG + chr4_4:73599994-73600013 None:intergenic 25.0%
!! TAAACAGAATCAGGTTATTT+TGG + chr4_4:73600142-73600161 None:intergenic 25.0%
! CATCTATATCATCTAACATT+TGG - chr4_4:73600217-73600236 Msa0702100:intron 25.0%
!! TTTTTCTAATGTAACCTAGA+CGG - chr4_4:73600954-73600973 Msa0702100:intron 25.0%
! CTAACATAGATAGAAATAGA+AGG + chr4_4:73601122-73601141 None:intergenic 25.0%
!!! AATAGAAGACATGTTATTTC+AGG - chr4_4:73601564-73601583 Msa0702100:five_prime_UTR 25.0%
GAATCAGAATCGATGAAATT+AGG - chr4_4:73599325-73599344 Msa0702100:three_prime_UTR 30.0%
AGTGTGTTGTAAATGGTTAA+GGG + chr4_4:73599407-73599426 None:intergenic 30.0%
TAGTGTGTTGTAAATGGTTA+AGG + chr4_4:73599408-73599427 None:intergenic 30.0%
CATACATAGTGTGTTGTAAA+TGG + chr4_4:73599414-73599433 None:intergenic 30.0%
AATTGAATTTCAGATTGGGA+AGG - chr4_4:73599521-73599540 Msa0702100:three_prime_UTR 30.0%
AAACAATGTAGCTCATAATC+TGG + chr4_4:73599885-73599904 None:intergenic 30.0%
TATTCTCTCGGAAAATTAAC+AGG - chr4_4:73599999-73600018 Msa0702100:intron 30.0%
!! ATCATCTCACATTTAGTTTG+TGG - chr4_4:73600248-73600267 Msa0702100:intron 30.0%
! AGAAGTTGCTGAAATTTTTC+TGG + chr4_4:73600305-73600324 None:intergenic 30.0%
TTGCATGCACTACATTATTA+GGG - chr4_4:73600336-73600355 Msa0702100:intron 30.0%
GAAAATGGAGAAAAACACAA+AGG - chr4_4:73600467-73600486 Msa0702100:intron 30.0%
GAGAAAAACACAAAGGATAT+AGG - chr4_4:73600474-73600493 Msa0702100:intron 30.0%
! ATCATTGGTGATAACAGTTA+AGG - chr4_4:73600525-73600544 Msa0702100:intron 30.0%
AACAGTTAAGGATTGACATT+AGG - chr4_4:73600537-73600556 Msa0702100:intron 30.0%
ACAGTTAAGGATTGACATTA+GGG - chr4_4:73600538-73600557 Msa0702100:intron 30.0%
! GTTGAAGATTAAGTTGTCTT+TGG - chr4_4:73600620-73600639 Msa0702100:intron 30.0%
TGATTACAAATTGACAGTGA+AGG - chr4_4:73600643-73600662 Msa0702100:intron 30.0%
TAGATAATAACAAGGCGATT+TGG - chr4_4:73600760-73600779 Msa0702100:intron 30.0%
ATTGGAAGTGATGACAAATA+TGG - chr4_4:73600882-73600901 Msa0702100:intron 30.0%
AAATCAGTTCAAGTCATCTA+AGG + chr4_4:73600930-73600949 None:intergenic 30.0%
!! GGTTGATAAACATATACTGA+TGG - chr4_4:73601020-73601039 Msa0702100:intron 30.0%
AGATGAACAGTAGAAGAAAA+AGG - chr4_4:73601054-73601073 Msa0702100:intron 30.0%
GATGAACAGTAGAAGAAAAA+GGG - chr4_4:73601055-73601074 Msa0702100:intron 30.0%
TGGATAAGTTGTTGAAAGTT+TGG + chr4_4:73601228-73601247 None:intergenic 30.0%
GATAACAGCAATAGTTACTT+AGG + chr4_4:73601311-73601330 None:intergenic 30.0%
CTTGAAATGCCTAAGTTATA+TGG - chr4_4:73601370-73601389 Msa0702100:intron 30.0%
TAAACACGTCCATATAACTT+AGG + chr4_4:73601382-73601401 None:intergenic 30.0%
AGAAATGAAGAAAACAATGC+AGG + chr4_4:73601452-73601471 None:intergenic 30.0%
TTCTTCATTTCTTGTCCAAA+AGG - chr4_4:73601460-73601479 Msa0702100:CDS 30.0%
! ATGCATGACTATTTTCCTTT+TGG + chr4_4:73601478-73601497 None:intergenic 30.0%
GAATCAGAATCGATGAAATT+AGG - chr4_4:73599325-73599344 Msa0702100:three_prime_UTR 30.0%
AGTGTGTTGTAAATGGTTAA+GGG + chr4_4:73599407-73599426 None:intergenic 30.0%
TAGTGTGTTGTAAATGGTTA+AGG + chr4_4:73599408-73599427 None:intergenic 30.0%
CATACATAGTGTGTTGTAAA+TGG + chr4_4:73599414-73599433 None:intergenic 30.0%
AATTGAATTTCAGATTGGGA+AGG - chr4_4:73599521-73599540 Msa0702100:three_prime_UTR 30.0%
AAACAATGTAGCTCATAATC+TGG + chr4_4:73599885-73599904 None:intergenic 30.0%
TATTCTCTCGGAAAATTAAC+AGG - chr4_4:73599999-73600018 Msa0702100:intron 30.0%
!! ATCATCTCACATTTAGTTTG+TGG - chr4_4:73600248-73600267 Msa0702100:intron 30.0%
! AGAAGTTGCTGAAATTTTTC+TGG + chr4_4:73600305-73600324 None:intergenic 30.0%
TTGCATGCACTACATTATTA+GGG - chr4_4:73600336-73600355 Msa0702100:intron 30.0%
GAAAATGGAGAAAAACACAA+AGG - chr4_4:73600467-73600486 Msa0702100:intron 30.0%
GAGAAAAACACAAAGGATAT+AGG - chr4_4:73600474-73600493 Msa0702100:intron 30.0%
! ATCATTGGTGATAACAGTTA+AGG - chr4_4:73600525-73600544 Msa0702100:intron 30.0%
AACAGTTAAGGATTGACATT+AGG - chr4_4:73600537-73600556 Msa0702100:intron 30.0%
ACAGTTAAGGATTGACATTA+GGG - chr4_4:73600538-73600557 Msa0702100:intron 30.0%
! GTTGAAGATTAAGTTGTCTT+TGG - chr4_4:73600620-73600639 Msa0702100:intron 30.0%
TGATTACAAATTGACAGTGA+AGG - chr4_4:73600643-73600662 Msa0702100:intron 30.0%
TAGATAATAACAAGGCGATT+TGG - chr4_4:73600760-73600779 Msa0702100:intron 30.0%
ATTGGAAGTGATGACAAATA+TGG - chr4_4:73600882-73600901 Msa0702100:intron 30.0%
AAATCAGTTCAAGTCATCTA+AGG + chr4_4:73600930-73600949 None:intergenic 30.0%
!! GGTTGATAAACATATACTGA+TGG - chr4_4:73601020-73601039 Msa0702100:intron 30.0%
AGATGAACAGTAGAAGAAAA+AGG - chr4_4:73601054-73601073 Msa0702100:intron 30.0%
GATGAACAGTAGAAGAAAAA+GGG - chr4_4:73601055-73601074 Msa0702100:intron 30.0%
TGGATAAGTTGTTGAAAGTT+TGG + chr4_4:73601228-73601247 None:intergenic 30.0%
GATAACAGCAATAGTTACTT+AGG + chr4_4:73601311-73601330 None:intergenic 30.0%
CTTGAAATGCCTAAGTTATA+TGG - chr4_4:73601370-73601389 Msa0702100:intron 30.0%
TAAACACGTCCATATAACTT+AGG + chr4_4:73601382-73601401 None:intergenic 30.0%
AGAAATGAAGAAAACAATGC+AGG + chr4_4:73601452-73601471 None:intergenic 30.0%
TTCTTCATTTCTTGTCCAAA+AGG - chr4_4:73601460-73601479 Msa0702100:CDS 30.0%
! ATGCATGACTATTTTCCTTT+TGG + chr4_4:73601478-73601497 None:intergenic 30.0%
AAAACGGTTTCATTTGGTCA+CGG - chr4_4:73599276-73599295 Msa0702100:three_prime_UTR 35.0%
ATCAAAGAAGCAATACAAGG+TGG - chr4_4:73599805-73599824 Msa0702100:intron 35.0%
! ACTCAAGTGTTAGTTTCGTT+AGG + chr4_4:73599916-73599935 None:intergenic 35.0%
ACTAACACTTGAGTGAGTTT+CGG - chr4_4:73599922-73599941 Msa0702100:intron 35.0%
AGTTTCGGAAAAGTGAAAGA+TGG - chr4_4:73599937-73599956 Msa0702100:intron 35.0%
GTTTCGGAAAAGTGAAAGAT+GGG - chr4_4:73599938-73599957 Msa0702100:intron 35.0%
GGATTTCAGAGCTATTATCA+AGG + chr4_4:73599973-73599992 None:intergenic 35.0%
! ATGATTCAGTTATGGACTGT+TGG - chr4_4:73600058-73600077 Msa0702100:intron 35.0%
!! CACATTTAGTTTGTGGCAAA+AGG - chr4_4:73600255-73600274 Msa0702100:intron 35.0%
!! AAAAGGTGCTACAATTGTTG+AGG - chr4_4:73600272-73600291 Msa0702100:intron 35.0%
GTTGCATGCACTACATTATT+AGG - chr4_4:73600335-73600354 Msa0702100:intron 35.0%
CATTATTAGGGATTCCTTTG+TGG - chr4_4:73600348-73600367 Msa0702100:intron 35.0%
! GGTCTTTGATCTTACTGTTT+TGG - chr4_4:73600418-73600437 Msa0702100:intron 35.0%
!! GGTGTTTTTGAACATTATGC+TGG - chr4_4:73600495-73600514 Msa0702100:intron 35.0%
TATGCTGGTGAAGTTATCAT+TGG - chr4_4:73600510-73600529 Msa0702100:intron 35.0%
CAGTTAAGGATTGACATTAG+GGG - chr4_4:73600539-73600558 Msa0702100:intron 35.0%
ACAGTGAAGGAGTTTCAATA+CGG - chr4_4:73600656-73600675 Msa0702100:intron 35.0%
CCAAAGACAACTTTACCATT+TGG + chr4_4:73600713-73600732 None:intergenic 35.0%
CCAAATGGTAAAGTTGTCTT+TGG - chr4_4:73600710-73600729 Msa0702100:intron 35.0%
AATGGTAAAGTTGTCTTTGG+TGG - chr4_4:73600713-73600732 Msa0702100:intron 35.0%
CACTAGAAGTCAAGTCAAAA+AGG - chr4_4:73600802-73600821 Msa0702100:intron 35.0%
CTTCTTCCTAAACCAAGAAT+TGG - chr4_4:73600864-73600883 Msa0702100:intron 35.0%
TCACTTCCAATTCTTGGTTT+AGG + chr4_4:73600873-73600892 None:intergenic 35.0%
TTGTCATCACTTCCAATTCT+TGG + chr4_4:73600879-73600898 None:intergenic 35.0%
! ACCTAGACGGTTTGTTTATT+AGG - chr4_4:73600967-73600986 Msa0702100:intron 35.0%
! CCTAGACGGTTTGTTTATTA+GGG - chr4_4:73600968-73600987 Msa0702100:intron 35.0%
AAACATATACTGATGGAGTG+AGG - chr4_4:73601027-73601046 Msa0702100:intron 35.0%
AACATATACTGATGGAGTGA+GGG - chr4_4:73601028-73601047 Msa0702100:intron 35.0%
ACAGTAGAAGAAAAAGGGTT+AGG - chr4_4:73601060-73601079 Msa0702100:intron 35.0%
ACGTTACTCATAGAATCTTC+AGG + chr4_4:73601260-73601279 None:intergenic 35.0%
CTATGAGTAACGTTATTGAC+AGG - chr4_4:73601267-73601286 Msa0702100:CDS 35.0%
TACTTAGGCATTTCAAGATC+CGG + chr4_4:73601296-73601315 None:intergenic 35.0%
AACTTAGGCATTTCAAGATC+CGG + chr4_4:73601367-73601386 None:intergenic 35.0%
TTGTTAGCAATGAAGTTCAG+GGG + chr4_4:73601528-73601547 None:intergenic 35.0%
CTTGTTAGCAATGAAGTTCA+GGG + chr4_4:73601529-73601548 None:intergenic 35.0%
! ACTTGTTAGCAATGAAGTTC+AGG + chr4_4:73601530-73601549 None:intergenic 35.0%
!! AGAAGACATGTTATTTCAGG+AGG - chr4_4:73601567-73601586 Msa0702100:five_prime_UTR 35.0%
AAAACGGTTTCATTTGGTCA+CGG - chr4_4:73599276-73599295 Msa0702100:three_prime_UTR 35.0%
ATCAAAGAAGCAATACAAGG+TGG - chr4_4:73599805-73599824 Msa0702100:intron 35.0%
! ACTCAAGTGTTAGTTTCGTT+AGG + chr4_4:73599916-73599935 None:intergenic 35.0%
ACTAACACTTGAGTGAGTTT+CGG - chr4_4:73599922-73599941 Msa0702100:intron 35.0%
AGTTTCGGAAAAGTGAAAGA+TGG - chr4_4:73599937-73599956 Msa0702100:intron 35.0%
GTTTCGGAAAAGTGAAAGAT+GGG - chr4_4:73599938-73599957 Msa0702100:intron 35.0%
GGATTTCAGAGCTATTATCA+AGG + chr4_4:73599973-73599992 None:intergenic 35.0%
! ATGATTCAGTTATGGACTGT+TGG - chr4_4:73600058-73600077 Msa0702100:intron 35.0%
!! CACATTTAGTTTGTGGCAAA+AGG - chr4_4:73600255-73600274 Msa0702100:intron 35.0%
!! AAAAGGTGCTACAATTGTTG+AGG - chr4_4:73600272-73600291 Msa0702100:intron 35.0%
GTTGCATGCACTACATTATT+AGG - chr4_4:73600335-73600354 Msa0702100:intron 35.0%
CATTATTAGGGATTCCTTTG+TGG - chr4_4:73600348-73600367 Msa0702100:intron 35.0%
! GGTCTTTGATCTTACTGTTT+TGG - chr4_4:73600418-73600437 Msa0702100:intron 35.0%
!! GGTGTTTTTGAACATTATGC+TGG - chr4_4:73600495-73600514 Msa0702100:intron 35.0%
TATGCTGGTGAAGTTATCAT+TGG - chr4_4:73600510-73600529 Msa0702100:intron 35.0%
CAGTTAAGGATTGACATTAG+GGG - chr4_4:73600539-73600558 Msa0702100:intron 35.0%
ACAGTGAAGGAGTTTCAATA+CGG - chr4_4:73600656-73600675 Msa0702100:intron 35.0%
CCAAAGACAACTTTACCATT+TGG + chr4_4:73600713-73600732 None:intergenic 35.0%
CCAAATGGTAAAGTTGTCTT+TGG - chr4_4:73600710-73600729 Msa0702100:intron 35.0%
AATGGTAAAGTTGTCTTTGG+TGG - chr4_4:73600713-73600732 Msa0702100:intron 35.0%
CACTAGAAGTCAAGTCAAAA+AGG - chr4_4:73600802-73600821 Msa0702100:intron 35.0%
CTTCTTCCTAAACCAAGAAT+TGG - chr4_4:73600864-73600883 Msa0702100:intron 35.0%
TCACTTCCAATTCTTGGTTT+AGG + chr4_4:73600873-73600892 None:intergenic 35.0%
TTGTCATCACTTCCAATTCT+TGG + chr4_4:73600879-73600898 None:intergenic 35.0%
! ACCTAGACGGTTTGTTTATT+AGG - chr4_4:73600967-73600986 Msa0702100:intron 35.0%
! CCTAGACGGTTTGTTTATTA+GGG - chr4_4:73600968-73600987 Msa0702100:intron 35.0%
AAACATATACTGATGGAGTG+AGG - chr4_4:73601027-73601046 Msa0702100:intron 35.0%
AACATATACTGATGGAGTGA+GGG - chr4_4:73601028-73601047 Msa0702100:intron 35.0%
ACAGTAGAAGAAAAAGGGTT+AGG - chr4_4:73601060-73601079 Msa0702100:intron 35.0%
ACGTTACTCATAGAATCTTC+AGG + chr4_4:73601260-73601279 None:intergenic 35.0%
CTATGAGTAACGTTATTGAC+AGG - chr4_4:73601267-73601286 Msa0702100:CDS 35.0%
TACTTAGGCATTTCAAGATC+CGG + chr4_4:73601296-73601315 None:intergenic 35.0%
AACTTAGGCATTTCAAGATC+CGG + chr4_4:73601367-73601386 None:intergenic 35.0%
TTGTTAGCAATGAAGTTCAG+GGG + chr4_4:73601528-73601547 None:intergenic 35.0%
CTTGTTAGCAATGAAGTTCA+GGG + chr4_4:73601529-73601548 None:intergenic 35.0%
! ACTTGTTAGCAATGAAGTTC+AGG + chr4_4:73601530-73601549 None:intergenic 35.0%
!! AGAAGACATGTTATTTCAGG+AGG - chr4_4:73601567-73601586 Msa0702100:five_prime_UTR 35.0%
GTTAAGGGAACGAAGATCAT+CGG + chr4_4:73599392-73599411 None:intergenic 40.0%
TGTAGCTCATAATCTGGCTA+AGG + chr4_4:73599879-73599898 None:intergenic 40.0%
!!! GTTATTTTGGATCGTGCAGA+GGG + chr4_4:73600129-73600148 None:intergenic 40.0%
!!! GCTGAAATTTTTCTGGAACC+TGG + chr4_4:73600298-73600317 None:intergenic 40.0%
ACACTAGAAACCAAGCCAAA+TGG - chr4_4:73600695-73600714 Msa0702100:intron 40.0%
GACAACTTTACCATTTGGCT+TGG + chr4_4:73600708-73600727 None:intergenic 40.0%
CTAGTGTTTACATGCTTCTC+TGG + chr4_4:73600788-73600807 None:intergenic 40.0%
CCCTAATAAACAAACCGTCT+AGG + chr4_4:73600971-73600990 None:intergenic 40.0%
ACCATTGTACCTAACCAGAA+CGG + chr4_4:73601003-73601022 None:intergenic 40.0%
! TCATGTTTCTGCAAAAGGCA+AGG + chr4_4:73601202-73601221 None:intergenic 40.0%
! GGTCTTCATGTTTCTGCAAA+AGG + chr4_4:73601207-73601226 None:intergenic 40.0%
GAATCTTCAGGAAATGCATC+TGG + chr4_4:73601248-73601267 None:intergenic 40.0%
TAACGTTATTGACAGGAGAC+CGG - chr4_4:73601274-73601293 Msa0702100:CDS 40.0%
GTTATTCGAACGTGTTTGAC+AGG - chr4_4:73601338-73601357 Msa0702100:intron 40.0%
GTTAAGGGAACGAAGATCAT+CGG + chr4_4:73599392-73599411 None:intergenic 40.0%
TGTAGCTCATAATCTGGCTA+AGG + chr4_4:73599879-73599898 None:intergenic 40.0%
!!! GTTATTTTGGATCGTGCAGA+GGG + chr4_4:73600129-73600148 None:intergenic 40.0%
!!! GCTGAAATTTTTCTGGAACC+TGG + chr4_4:73600298-73600317 None:intergenic 40.0%
ACACTAGAAACCAAGCCAAA+TGG - chr4_4:73600695-73600714 Msa0702100:intron 40.0%
GACAACTTTACCATTTGGCT+TGG + chr4_4:73600708-73600727 None:intergenic 40.0%
CTAGTGTTTACATGCTTCTC+TGG + chr4_4:73600788-73600807 None:intergenic 40.0%
CCCTAATAAACAAACCGTCT+AGG + chr4_4:73600971-73600990 None:intergenic 40.0%
ACCATTGTACCTAACCAGAA+CGG + chr4_4:73601003-73601022 None:intergenic 40.0%
! TCATGTTTCTGCAAAAGGCA+AGG + chr4_4:73601202-73601221 None:intergenic 40.0%
! GGTCTTCATGTTTCTGCAAA+AGG + chr4_4:73601207-73601226 None:intergenic 40.0%
GAATCTTCAGGAAATGCATC+TGG + chr4_4:73601248-73601267 None:intergenic 40.0%
TAACGTTATTGACAGGAGAC+CGG - chr4_4:73601274-73601293 Msa0702100:CDS 40.0%
GTTATTCGAACGTGTTTGAC+AGG - chr4_4:73601338-73601357 Msa0702100:intron 40.0%
TTTCAGATTGGGAAGGTTGC+AGG - chr4_4:73599528-73599547 Msa0702100:three_prime_UTR 45.0%
TACCCAGATTTCTCCTGACA+TGG - chr4_4:73599600-73599619 Msa0702100:CDS 45.0%
AGCCATGTCAGGAGAAATCT+GGG + chr4_4:73599605-73599624 None:intergenic 45.0%
TAGCCATGTCAGGAGAAATC+TGG + chr4_4:73599606-73599625 None:intergenic 45.0%
!!! GGTTATTTTGGATCGTGCAG+AGG + chr4_4:73600130-73600149 None:intergenic 45.0%
TCAGCAACTTCTGCAAGTTG+AGG - chr4_4:73600313-73600332 Msa0702100:intron 45.0%
GGATTCCTTTGTGGTTCATG+CGG - chr4_4:73600357-73600376 Msa0702100:intron 45.0%
GATTCCTTTGTGGTTCATGC+GGG - chr4_4:73600358-73600377 Msa0702100:intron 45.0%
ATTCCTTTGTGGTTCATGCG+GGG - chr4_4:73600359-73600378 Msa0702100:intron 45.0%
TTGACATTAGGGGCAACAAC+AGG - chr4_4:73600549-73600568 Msa0702100:intron 45.0%
TGACATTAGGGGCAACAACA+GGG - chr4_4:73600550-73600569 Msa0702100:intron 45.0%
CTTGCAAACACAGCAGCAAA+AGG - chr4_4:73600830-73600849 Msa0702100:intron 45.0%
! CTATCTTACCCGTTCTGGTT+AGG - chr4_4:73600991-73601010 Msa0702100:intron 45.0%
CCATTGTACCTAACCAGAAC+GGG + chr4_4:73601002-73601021 None:intergenic 45.0%
GAAGTTCAGGGGCAAGAAAT+TGG + chr4_4:73601517-73601536 None:intergenic 45.0%
TTTCAGATTGGGAAGGTTGC+AGG - chr4_4:73599528-73599547 Msa0702100:three_prime_UTR 45.0%
TACCCAGATTTCTCCTGACA+TGG - chr4_4:73599600-73599619 Msa0702100:CDS 45.0%
AGCCATGTCAGGAGAAATCT+GGG + chr4_4:73599605-73599624 None:intergenic 45.0%
TAGCCATGTCAGGAGAAATC+TGG + chr4_4:73599606-73599625 None:intergenic 45.0%
!!! GGTTATTTTGGATCGTGCAG+AGG + chr4_4:73600130-73600149 None:intergenic 45.0%
TCAGCAACTTCTGCAAGTTG+AGG - chr4_4:73600313-73600332 Msa0702100:intron 45.0%
GGATTCCTTTGTGGTTCATG+CGG - chr4_4:73600357-73600376 Msa0702100:intron 45.0%
GATTCCTTTGTGGTTCATGC+GGG - chr4_4:73600358-73600377 Msa0702100:intron 45.0%
ATTCCTTTGTGGTTCATGCG+GGG - chr4_4:73600359-73600378 Msa0702100:intron 45.0%
TTGACATTAGGGGCAACAAC+AGG - chr4_4:73600549-73600568 Msa0702100:intron 45.0%
TGACATTAGGGGCAACAACA+GGG - chr4_4:73600550-73600569 Msa0702100:intron 45.0%
CTTGCAAACACAGCAGCAAA+AGG - chr4_4:73600830-73600849 Msa0702100:intron 45.0%
! CTATCTTACCCGTTCTGGTT+AGG - chr4_4:73600991-73601010 Msa0702100:intron 45.0%
CCATTGTACCTAACCAGAAC+GGG + chr4_4:73601002-73601021 None:intergenic 45.0%
GAAGTTCAGGGGCAAGAAAT+TGG + chr4_4:73601517-73601536 None:intergenic 45.0%
!! CGTGTGTTTCTAGCCATGTC+AGG + chr4_4:73599616-73599635 None:intergenic 50.0%
TACTGACCTCCCTCAAGAGT+TGG - chr4_4:73600083-73600102 Msa0702100:intron 50.0%
ACTGACCTCCCTCAAGAGTT+GGG - chr4_4:73600084-73600103 Msa0702100:intron 50.0%
GTGCTACAATTGTTGAGGCC+AGG - chr4_4:73600277-73600296 Msa0702100:intron 50.0%
TTACCCCGCATGAACCACAA+AGG + chr4_4:73600365-73600384 None:intergenic 50.0%
GACATTAGGGGCAACAACAG+GGG - chr4_4:73600551-73600570 Msa0702100:intron 50.0%
TAGGGCTATCTTACCCGTTC+TGG - chr4_4:73600986-73601005 Msa0702100:intron 50.0%
! CCCGTTCTGGTTAGGTACAA+TGG - chr4_4:73600999-73601018 Msa0702100:intron 50.0%
GAACGTGTTTGACAGGAGAC+CGG - chr4_4:73601345-73601364 Msa0702100:intron 50.0%
!! CGTGTGTTTCTAGCCATGTC+AGG + chr4_4:73599616-73599635 None:intergenic 50.0%
TACTGACCTCCCTCAAGAGT+TGG - chr4_4:73600083-73600102 Msa0702100:intron 50.0%
ACTGACCTCCCTCAAGAGTT+GGG - chr4_4:73600084-73600103 Msa0702100:intron 50.0%
GTGCTACAATTGTTGAGGCC+AGG - chr4_4:73600277-73600296 Msa0702100:intron 50.0%
TTACCCCGCATGAACCACAA+AGG + chr4_4:73600365-73600384 None:intergenic 50.0%
GACATTAGGGGCAACAACAG+GGG - chr4_4:73600551-73600570 Msa0702100:intron 50.0%
TAGGGCTATCTTACCCGTTC+TGG - chr4_4:73600986-73601005 Msa0702100:intron 50.0%
! CCCGTTCTGGTTAGGTACAA+TGG - chr4_4:73600999-73601018 Msa0702100:intron 50.0%
GAACGTGTTTGACAGGAGAC+CGG - chr4_4:73601345-73601364 Msa0702100:intron 50.0%
GACCTCCCTCAAGAGTTGGG+AGG - chr4_4:73600087-73600106 Msa0702100:intron 60.0%
TGCCTCCCAACTCTTGAGGG+AGG + chr4_4:73600092-73600111 None:intergenic 60.0%
TCCCTCAAGAGTTGGGAGGC+AGG - chr4_4:73600091-73600110 Msa0702100:intron 60.0%
GCCTGCCTCCCAACTCTTGA+GGG + chr4_4:73600095-73600114 None:intergenic 60.0%
TGCCTGCCTCCCAACTCTTG+AGG + chr4_4:73600096-73600115 None:intergenic 60.0%
GAGTTGGGAGGCAGGCAGTA+TGG - chr4_4:73600099-73600118 Msa0702100:intron 60.0%
GACCTCCCTCAAGAGTTGGG+AGG - chr4_4:73600087-73600106 Msa0702100:intron 60.0%
TGCCTCCCAACTCTTGAGGG+AGG + chr4_4:73600092-73600111 None:intergenic 60.0%
TCCCTCAAGAGTTGGGAGGC+AGG - chr4_4:73600091-73600110 Msa0702100:intron 60.0%
GCCTGCCTCCCAACTCTTGA+GGG + chr4_4:73600095-73600114 None:intergenic 60.0%
TGCCTGCCTCCCAACTCTTG+AGG + chr4_4:73600096-73600115 None:intergenic 60.0%
GAGTTGGGAGGCAGGCAGTA+TGG - chr4_4:73600099-73600118 Msa0702100:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 73599273 73601596 73599273 ID=Msa0702100;Name=Msa0702100
chr4_4 mRNA 73599273 73601596 73599273 ID=Msa0702100-mRNA-1;Parent=Msa0702100;Name=Msa0702100-mRNA-1;_AED=0.08;_eAED=0.08;_QI=34|1|1|1|1|1|3|280|125
chr4_4 exon 73599273 73599655 73599273 ID=Msa0702100-mRNA-1:exon:22728;Parent=Msa0702100-mRNA-1
chr4_4 exon 73601183 73601335 73601183 ID=Msa0702100-mRNA-1:exon:22727;Parent=Msa0702100-mRNA-1
chr4_4 exon 73601441 73601596 73601441 ID=Msa0702100-mRNA-1:exon:22726;Parent=Msa0702100-mRNA-1
chr4_4 five_prime_UTR 73601563 73601596 73601563 ID=Msa0702100-mRNA-1:five_prime_utr;Parent=Msa0702100-mRNA-1
chr4_4 CDS 73601441 73601562 73601441 ID=Msa0702100-mRNA-1:cds;Parent=Msa0702100-mRNA-1
chr4_4 CDS 73601183 73601335 73601183 ID=Msa0702100-mRNA-1:cds;Parent=Msa0702100-mRNA-1
chr4_4 CDS 73599553 73599655 73599553 ID=Msa0702100-mRNA-1:cds;Parent=Msa0702100-mRNA-1
chr4_4 three_prime_UTR 73599273 73599552 73599273 ID=Msa0702100-mRNA-1:three_prime_utr;Parent=Msa0702100-mRNA-1
Gene Sequence

>Msa0702100

ATGTCAGAGAAAGAATCAGAATCAGAATCGATGAAATTAGGTTCAGAAGAGCTTTCGAGTGAGTTCAAAACTCTCGTTAGTTCCGATGATCTTCGTTCCCTTAACCATTTACAACACACTATATTGGGAAGGTTGCAGGATAGTAATGCAGTGTTATCACATTTCAATGATTTCTCTCAACATTGTTTTACCCAGATTTCTCCTGACATGGCTAGAAACACACGTGTCTTGAAATCTATCAAATCTGATCTTGATTATATTTTTCTTAAACTTAGAAACATGAAGACCAAACTTTCAACAACTTATCCAGATGCATTTCCTGAAGATTCTATGAGTAACGTTATTGACAGGAGACCGGATCTTGAAATGCCTAAGTAA
Protein sequence

>Msa0702100

MSEKESESESMKLGSEELSSEFKTLVSSDDLRSLNHLQHTILGRLQDSNAVLSHFNDFSQHCFTQISPDMARNTRVLKSIKSDLDYIFLKLRNMKTKLSTTYPDAFPEDSMSNVIDRRPDLEMPK*