AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0657790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0657790 MtrunA17_Chr8g0380411 98.374 123 2 0 3 125 5 127 2.98e-84 244
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0657790 AT3G29130.1 64.103 117 42 0 9 125 3 119 4.01e-55 168
Msa0657790 AT3G29130.6 64.103 117 42 0 9 125 3 119 4.01e-55 168
Msa0657790 AT3G29130.5 64.103 117 42 0 9 125 3 119 4.01e-55 168
Msa0657790 AT3G29130.2 64.103 117 42 0 9 125 52 168 1.05e-54 169
Msa0657790 AT3G29130.4 65.000 80 28 0 46 125 12 91 9.97e-37 120
Msa0657790 AT3G29130.3 65.000 80 28 0 46 125 12 91 9.97e-37 120

Find 33 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATACATAGTGTGTTGTAAA+TGG 0.303412 4_3:-67687653 None:intergenic
TGGATAAGTTGTTGAAAGTT+TGG 0.311208 4_3:-67689505 None:intergenic
AATAGAAGACATGTTATTTC+AGG 0.314826 4_3:+67689844 Msa0657790:three_prime_UTR
GAATCAGAATCGATGAAATT+AGG 0.315765 4_3:+67687567 Msa0657790:CDS
CTTGAAATGCCTAAGTTATA+TGG 0.324858 4_3:+67689650 Msa0657790:three_prime_UTR
GAAGTTCAGGGGCAAGAAAT+TGG 0.328764 4_3:-67689794 None:intergenic
ACTTGTTAGCAATGAAGTTC+AGG 0.339437 4_3:-67689807 None:intergenic
TAGTGTGTTGTAAATGGTTA+AGG 0.342554 4_3:-67687647 None:intergenic
TTTCAGATTGGGAAGGTTGC+AGG 0.354404 4_3:+67687770 Msa0657790:intron
TACTTAGGCATTTCAAGATC+CGG 0.393355 4_3:-67689573 None:intergenic
TAGCCATGTCAGGAGAAATC+TGG 0.395052 4_3:-67687845 None:intergenic
TTCTTCATTTCTTGTCCAAA+AGG 0.409409 4_3:+67689740 Msa0657790:three_prime_UTR
TCAATAATTCATTATAACTA+AGG 0.414895 4_3:-67689697 None:intergenic
AACTTAGGCATTTCAAGATC+CGG 0.422372 4_3:-67689644 None:intergenic
ACGTTACTCATAGAATCTTC+AGG 0.435689 4_3:-67689537 None:intergenic
GAATCTTCAGGAAATGCATC+TGG 0.446985 4_3:-67689525 None:intergenic
GTTATTCGAACGTGTTTGAC+AGG 0.458228 4_3:+67689618 Msa0657790:three_prime_UTR
GATAACAGCAATAGTTACTT+AGG 0.475331 4_3:-67689588 None:intergenic
AGTGTGTTGTAAATGGTTAA+GGG 0.496258 4_3:-67687646 None:intergenic
CGTGTGTTTCTAGCCATGTC+AGG 0.515825 4_3:-67687855 None:intergenic
CTTGTTAGCAATGAAGTTCA+GGG 0.517721 4_3:-67689806 None:intergenic
GAACGTGTTTGACAGGAGAC+CGG 0.536487 4_3:+67689625 Msa0657790:three_prime_UTR
CTATGAGTAACGTTATTGAC+AGG 0.539299 4_3:+67689547 Msa0657790:CDS
TACCCAGATTTCTCCTGACA+TGG 0.542515 4_3:+67687842 Msa0657790:CDS
TAAACACGTCCATATAACTT+AGG 0.553646 4_3:-67689659 None:intergenic
AGCCATGTCAGGAGAAATCT+GGG 0.559247 4_3:-67687844 None:intergenic
AGAAATGAAGAAAACAATGC+AGG 0.559601 4_3:-67689729 None:intergenic
AAAACGGTTTCATTTGGTCA+CGG 0.573588 4_3:+67687518 Msa0657790:five_prime_UTR
AGAAGACATGTTATTTCAGG+AGG 0.574015 4_3:+67689847 Msa0657790:three_prime_UTR
TAACGTTATTGACAGGAGAC+CGG 0.586364 4_3:+67689554 Msa0657790:CDS
AATTGAATTTCAGATTGGGA+AGG 0.586674 4_3:+67687763 Msa0657790:intron
GTTAAGGGAACGAAGATCAT+CGG 0.608518 4_3:-67687631 None:intergenic
TTGTTAGCAATGAAGTTCAG+GGG 0.652292 4_3:-67689805 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATATTTTTCTTAAACTT+AGG + chr4_3:67687907-67687926 Msa0657790:CDS 10.0%
!! ATATCATCAAATTATATAAT+TGG + chr4_3:67687971-67687990 Msa0657790:intron 10.0%
!!! TTATTGAAAATTCTTAATTT+TGG + chr4_3:67688401-67688420 Msa0657790:intron 10.0%
!!! ATTATATTTTTCTTAAACTT+AGG + chr4_3:67687907-67687926 Msa0657790:CDS 10.0%
!! ATATCATCAAATTATATAAT+TGG + chr4_3:67687971-67687990 Msa0657790:intron 10.0%
!!! TTATTGAAAATTCTTAATTT+TGG + chr4_3:67688401-67688420 Msa0657790:intron 10.0%
!!! AGTTTGTATGTATATTTTTT+AGG + chr4_3:67687997-67688016 Msa0657790:intron 15.0%
!! TTTACAATTAGATAATAACA+AGG + chr4_3:67689032-67689051 Msa0657790:intron 15.0%
!! TCAATAATTCATTATAACTA+AGG - chr4_3:67689700-67689719 None:intergenic 15.0%
!!! AGTTTGTATGTATATTTTTT+AGG + chr4_3:67687997-67688016 Msa0657790:intron 15.0%
!! TTTACAATTAGATAATAACA+AGG + chr4_3:67689032-67689051 Msa0657790:intron 15.0%
!! TCAATAATTCATTATAACTA+AGG - chr4_3:67689700-67689719 None:intergenic 15.0%
!! AATTGAATTGAATTTCAGAT+TGG + chr4_3:67687758-67687777 Msa0657790:intron 20.0%
!! ATTGAATTGAATTTCAGATT+GGG + chr4_3:67687759-67687778 Msa0657790:intron 20.0%
!!! TGGAAATTGATAATAATCTT+GGG + chr4_3:67688067-67688086 Msa0657790:intron 20.0%
!! AAAATAGTATGATTCAGTTA+TGG + chr4_3:67688292-67688311 Msa0657790:intron 20.0%
!!! AATTTTCAATAAACAGAATC+AGG - chr4_3:67688393-67688412 None:intergenic 20.0%
!!! GTTTAGACTAAATATAGTTT+TGG + chr4_3:67688677-67688696 Msa0657790:intron 20.0%
!!! CTAACAAAATTTTGAGAAAA+TGG + chr4_3:67688732-67688751 Msa0657790:intron 20.0%
!!! TTAATCTTCAACATTTTACT+TGG - chr4_3:67688892-67688911 None:intergenic 20.0%
!! AATTGAATTGAATTTCAGAT+TGG + chr4_3:67687758-67687777 Msa0657790:intron 20.0%
!! ATTGAATTGAATTTCAGATT+GGG + chr4_3:67687759-67687778 Msa0657790:intron 20.0%
!!! TGGAAATTGATAATAATCTT+GGG + chr4_3:67688067-67688086 Msa0657790:intron 20.0%
!! AAAATAGTATGATTCAGTTA+TGG + chr4_3:67688292-67688311 Msa0657790:intron 20.0%
!!! AATTTTCAATAAACAGAATC+AGG - chr4_3:67688393-67688412 None:intergenic 20.0%
!!! GTTTAGACTAAATATAGTTT+TGG + chr4_3:67688677-67688696 Msa0657790:intron 20.0%
!!! CTAACAAAATTTTGAGAAAA+TGG + chr4_3:67688732-67688751 Msa0657790:intron 20.0%
!!! TTAATCTTCAACATTTTACT+TGG - chr4_3:67688892-67688911 None:intergenic 20.0%
! AAAAAAAAGAGAGATGAAAG+AGG - chr4_3:67687690-67687709 None:intergenic 25.0%
! AAAATCAAAGAAGCAATACA+AGG + chr4_3:67688044-67688063 Msa0657790:intron 25.0%
!! GTGGAAATTGATAATAATCT+TGG + chr4_3:67688066-67688085 Msa0657790:intron 25.0%
!! GGAAATTGATAATAATCTTG+GGG + chr4_3:67688068-67688087 Msa0657790:intron 25.0%
!! GAAATTGATAATAATCTTGG+GGG + chr4_3:67688069-67688088 Msa0657790:intron 25.0%
!! AAATCCGAATTTTATTCTCT+CGG + chr4_3:67688229-67688248 Msa0657790:intron 25.0%
!! TTTTCCGAGAGAATAAAATT+CGG - chr4_3:67688236-67688255 None:intergenic 25.0%
!! TAAACAGAATCAGGTTATTT+TGG - chr4_3:67688384-67688403 None:intergenic 25.0%
!! TTTTTCTAATGTAACCTAGA+CGG + chr4_3:67689234-67689253 Msa0657790:intron 25.0%
! CTAACATAGATAGAAATAGA+AGG - chr4_3:67689402-67689421 None:intergenic 25.0%
!!! AATAGAAGACATGTTATTTC+AGG + chr4_3:67689844-67689863 Msa0657790:three_prime_UTR 25.0%
! AAAAAAAAGAGAGATGAAAG+AGG - chr4_3:67687690-67687709 None:intergenic 25.0%
! AAAATCAAAGAAGCAATACA+AGG + chr4_3:67688044-67688063 Msa0657790:intron 25.0%
!! GTGGAAATTGATAATAATCT+TGG + chr4_3:67688066-67688085 Msa0657790:intron 25.0%
!! GGAAATTGATAATAATCTTG+GGG + chr4_3:67688068-67688087 Msa0657790:intron 25.0%
!! GAAATTGATAATAATCTTGG+GGG + chr4_3:67688069-67688088 Msa0657790:intron 25.0%
!! AAATCCGAATTTTATTCTCT+CGG + chr4_3:67688229-67688248 Msa0657790:intron 25.0%
!! TTTTCCGAGAGAATAAAATT+CGG - chr4_3:67688236-67688255 None:intergenic 25.0%
!! TAAACAGAATCAGGTTATTT+TGG - chr4_3:67688384-67688403 None:intergenic 25.0%
!! TTTTTCTAATGTAACCTAGA+CGG + chr4_3:67689234-67689253 Msa0657790:intron 25.0%
! CTAACATAGATAGAAATAGA+AGG - chr4_3:67689402-67689421 None:intergenic 25.0%
!!! AATAGAAGACATGTTATTTC+AGG + chr4_3:67689844-67689863 Msa0657790:three_prime_UTR 25.0%
GAATCAGAATCGATGAAATT+AGG + chr4_3:67687567-67687586 Msa0657790:CDS 30.0%
AGTGTGTTGTAAATGGTTAA+GGG - chr4_3:67687649-67687668 None:intergenic 30.0%
TAGTGTGTTGTAAATGGTTA+AGG - chr4_3:67687650-67687669 None:intergenic 30.0%
CATACATAGTGTGTTGTAAA+TGG - chr4_3:67687656-67687675 None:intergenic 30.0%
AATTGAATTTCAGATTGGGA+AGG + chr4_3:67687763-67687782 Msa0657790:intron 30.0%
AAACAATGTAGCTCATAATC+TGG - chr4_3:67688127-67688146 None:intergenic 30.0%
TATTCTCTCGGAAAATTAAC+AGG + chr4_3:67688241-67688260 Msa0657790:intron 30.0%
CATCTATATCACCTAACATT+TGG + chr4_3:67688459-67688478 Msa0657790:intron 30.0%
! AGAAGTTGCTGAAATTTTTC+TGG - chr4_3:67688585-67688604 None:intergenic 30.0%
TTGCATGCACTACATTATTA+GGG + chr4_3:67688616-67688635 Msa0657790:intron 30.0%
GAAAATGGAGAAAAACACAA+AGG + chr4_3:67688747-67688766 Msa0657790:intron 30.0%
GAGAAAAACACAAAGGATAT+AGG + chr4_3:67688754-67688773 Msa0657790:intron 30.0%
! ATCATTGGTGATAACAGTTA+AGG + chr4_3:67688805-67688824 Msa0657790:intron 30.0%
AACAGTTAAGGATTGACATT+AGG + chr4_3:67688817-67688836 Msa0657790:intron 30.0%
ACAGTTAAGGATTGACATTA+GGG + chr4_3:67688818-67688837 Msa0657790:intron 30.0%
! GTTGAAGATTAAGTTGTCTT+TGG + chr4_3:67688900-67688919 Msa0657790:intron 30.0%
TGATTACAAATTGACAGTGA+AGG + chr4_3:67688923-67688942 Msa0657790:intron 30.0%
TAGATAATAACAAGGCGATT+TGG + chr4_3:67689040-67689059 Msa0657790:intron 30.0%
ATTGGAAGTGATGACAAATA+TGG + chr4_3:67689162-67689181 Msa0657790:intron 30.0%
AAATCAGTTCAAGTCATCTA+AGG - chr4_3:67689210-67689229 None:intergenic 30.0%
!! GGTTGATAAACATATACTGA+TGG + chr4_3:67689300-67689319 Msa0657790:intron 30.0%
AGATGAACAGTAGAAGAAAA+AGG + chr4_3:67689334-67689353 Msa0657790:intron 30.0%
GATGAACAGTAGAAGAAAAA+GGG + chr4_3:67689335-67689354 Msa0657790:intron 30.0%
TGGATAAGTTGTTGAAAGTT+TGG - chr4_3:67689508-67689527 None:intergenic 30.0%
GATAACAGCAATAGTTACTT+AGG - chr4_3:67689591-67689610 None:intergenic 30.0%
CTTGAAATGCCTAAGTTATA+TGG + chr4_3:67689650-67689669 Msa0657790:three_prime_UTR 30.0%
TAAACACGTCCATATAACTT+AGG - chr4_3:67689662-67689681 None:intergenic 30.0%
AGAAATGAAGAAAACAATGC+AGG - chr4_3:67689732-67689751 None:intergenic 30.0%
TTCTTCATTTCTTGTCCAAA+AGG + chr4_3:67689740-67689759 Msa0657790:three_prime_UTR 30.0%
! ATGCATGACTATTTTCCTTT+TGG - chr4_3:67689758-67689777 None:intergenic 30.0%
GAATCAGAATCGATGAAATT+AGG + chr4_3:67687567-67687586 Msa0657790:CDS 30.0%
AGTGTGTTGTAAATGGTTAA+GGG - chr4_3:67687649-67687668 None:intergenic 30.0%
TAGTGTGTTGTAAATGGTTA+AGG - chr4_3:67687650-67687669 None:intergenic 30.0%
CATACATAGTGTGTTGTAAA+TGG - chr4_3:67687656-67687675 None:intergenic 30.0%
AATTGAATTTCAGATTGGGA+AGG + chr4_3:67687763-67687782 Msa0657790:intron 30.0%
AAACAATGTAGCTCATAATC+TGG - chr4_3:67688127-67688146 None:intergenic 30.0%
TATTCTCTCGGAAAATTAAC+AGG + chr4_3:67688241-67688260 Msa0657790:intron 30.0%
CATCTATATCACCTAACATT+TGG + chr4_3:67688459-67688478 Msa0657790:intron 30.0%
! AGAAGTTGCTGAAATTTTTC+TGG - chr4_3:67688585-67688604 None:intergenic 30.0%
TTGCATGCACTACATTATTA+GGG + chr4_3:67688616-67688635 Msa0657790:intron 30.0%
GAAAATGGAGAAAAACACAA+AGG + chr4_3:67688747-67688766 Msa0657790:intron 30.0%
GAGAAAAACACAAAGGATAT+AGG + chr4_3:67688754-67688773 Msa0657790:intron 30.0%
! ATCATTGGTGATAACAGTTA+AGG + chr4_3:67688805-67688824 Msa0657790:intron 30.0%
AACAGTTAAGGATTGACATT+AGG + chr4_3:67688817-67688836 Msa0657790:intron 30.0%
ACAGTTAAGGATTGACATTA+GGG + chr4_3:67688818-67688837 Msa0657790:intron 30.0%
! GTTGAAGATTAAGTTGTCTT+TGG + chr4_3:67688900-67688919 Msa0657790:intron 30.0%
TGATTACAAATTGACAGTGA+AGG + chr4_3:67688923-67688942 Msa0657790:intron 30.0%
TAGATAATAACAAGGCGATT+TGG + chr4_3:67689040-67689059 Msa0657790:intron 30.0%
ATTGGAAGTGATGACAAATA+TGG + chr4_3:67689162-67689181 Msa0657790:intron 30.0%
AAATCAGTTCAAGTCATCTA+AGG - chr4_3:67689210-67689229 None:intergenic 30.0%
!! GGTTGATAAACATATACTGA+TGG + chr4_3:67689300-67689319 Msa0657790:intron 30.0%
AGATGAACAGTAGAAGAAAA+AGG + chr4_3:67689334-67689353 Msa0657790:intron 30.0%
GATGAACAGTAGAAGAAAAA+GGG + chr4_3:67689335-67689354 Msa0657790:intron 30.0%
TGGATAAGTTGTTGAAAGTT+TGG - chr4_3:67689508-67689527 None:intergenic 30.0%
GATAACAGCAATAGTTACTT+AGG - chr4_3:67689591-67689610 None:intergenic 30.0%
CTTGAAATGCCTAAGTTATA+TGG + chr4_3:67689650-67689669 Msa0657790:three_prime_UTR 30.0%
TAAACACGTCCATATAACTT+AGG - chr4_3:67689662-67689681 None:intergenic 30.0%
AGAAATGAAGAAAACAATGC+AGG - chr4_3:67689732-67689751 None:intergenic 30.0%
TTCTTCATTTCTTGTCCAAA+AGG + chr4_3:67689740-67689759 Msa0657790:three_prime_UTR 30.0%
! ATGCATGACTATTTTCCTTT+TGG - chr4_3:67689758-67689777 None:intergenic 30.0%
AAAACGGTTTCATTTGGTCA+CGG + chr4_3:67687518-67687537 Msa0657790:five_prime_UTR 35.0%
ATCAAAGAAGCAATACAAGG+TGG + chr4_3:67688047-67688066 Msa0657790:intron 35.0%
! ACTCAAGTGTTAGTTTCGTT+AGG - chr4_3:67688158-67688177 None:intergenic 35.0%
ACTAACACTTGAGTGAGTTT+CGG + chr4_3:67688164-67688183 Msa0657790:intron 35.0%
AGTTTCGGAAAAGTGAAAGA+TGG + chr4_3:67688179-67688198 Msa0657790:intron 35.0%
GTTTCGGAAAAGTGAAAGAT+GGG + chr4_3:67688180-67688199 Msa0657790:intron 35.0%
GGATTTCAGAGCTATTATCA+AGG - chr4_3:67688215-67688234 None:intergenic 35.0%
! ATGATTCAGTTATGGACTGT+TGG + chr4_3:67688300-67688319 Msa0657790:intron 35.0%
GATAAACATCGCCAAATGTT+AGG - chr4_3:67688473-67688492 None:intergenic 35.0%
! CTTTTGCCACAAACTAAAGT+GGG - chr4_3:67688537-67688556 None:intergenic 35.0%
!! AAAAGGTGCTACAATTGTTG+AGG + chr4_3:67688552-67688571 Msa0657790:intron 35.0%
GTTGCATGCACTACATTATT+AGG + chr4_3:67688615-67688634 Msa0657790:intron 35.0%
CATTATTAGGGATTCCTTTG+TGG + chr4_3:67688628-67688647 Msa0657790:intron 35.0%
! GGTCTTTGATCTTACTGTTT+TGG + chr4_3:67688698-67688717 Msa0657790:intron 35.0%
!! GGTGTTTTTGAACATTATGC+TGG + chr4_3:67688775-67688794 Msa0657790:intron 35.0%
TATGCTGGTGAAGTTATCAT+TGG + chr4_3:67688790-67688809 Msa0657790:intron 35.0%
CAGTTAAGGATTGACATTAG+GGG + chr4_3:67688819-67688838 Msa0657790:intron 35.0%
ACAGTGAAGGAGTTTCAATA+CGG + chr4_3:67688936-67688955 Msa0657790:intron 35.0%
CCAAAGACAACTTTACCATT+TGG - chr4_3:67688993-67689012 None:intergenic 35.0%
CCAAATGGTAAAGTTGTCTT+TGG + chr4_3:67688990-67689009 Msa0657790:intron 35.0%
AATGGTAAAGTTGTCTTTGG+TGG + chr4_3:67688993-67689012 Msa0657790:intron 35.0%
CACTAGAAGTCAAGTCAAAA+AGG + chr4_3:67689082-67689101 Msa0657790:intron 35.0%
CTTCTTCCTAAACCAAGAAT+TGG + chr4_3:67689144-67689163 Msa0657790:intron 35.0%
TCACTTCCAATTCTTGGTTT+AGG - chr4_3:67689153-67689172 None:intergenic 35.0%
TTGTCATCACTTCCAATTCT+TGG - chr4_3:67689159-67689178 None:intergenic 35.0%
! ACCTAGACGGTTTGTTTATT+AGG + chr4_3:67689247-67689266 Msa0657790:intron 35.0%
! CCTAGACGGTTTGTTTATTA+GGG + chr4_3:67689248-67689267 Msa0657790:intron 35.0%
AAACATATACTGATGGAGTG+AGG + chr4_3:67689307-67689326 Msa0657790:intron 35.0%
AACATATACTGATGGAGTGA+GGG + chr4_3:67689308-67689327 Msa0657790:intron 35.0%
ACAGTAGAAGAAAAAGGGTT+AGG + chr4_3:67689340-67689359 Msa0657790:intron 35.0%
ACGTTACTCATAGAATCTTC+AGG - chr4_3:67689540-67689559 None:intergenic 35.0%
CTATGAGTAACGTTATTGAC+AGG + chr4_3:67689547-67689566 Msa0657790:CDS 35.0%
TACTTAGGCATTTCAAGATC+CGG - chr4_3:67689576-67689595 None:intergenic 35.0%
AACTTAGGCATTTCAAGATC+CGG - chr4_3:67689647-67689666 None:intergenic 35.0%
TTGTTAGCAATGAAGTTCAG+GGG - chr4_3:67689808-67689827 None:intergenic 35.0%
CTTGTTAGCAATGAAGTTCA+GGG - chr4_3:67689809-67689828 None:intergenic 35.0%
! ACTTGTTAGCAATGAAGTTC+AGG - chr4_3:67689810-67689829 None:intergenic 35.0%
!! AGAAGACATGTTATTTCAGG+AGG + chr4_3:67689847-67689866 Msa0657790:three_prime_UTR 35.0%
AAAACGGTTTCATTTGGTCA+CGG + chr4_3:67687518-67687537 Msa0657790:five_prime_UTR 35.0%
ATCAAAGAAGCAATACAAGG+TGG + chr4_3:67688047-67688066 Msa0657790:intron 35.0%
! ACTCAAGTGTTAGTTTCGTT+AGG - chr4_3:67688158-67688177 None:intergenic 35.0%
ACTAACACTTGAGTGAGTTT+CGG + chr4_3:67688164-67688183 Msa0657790:intron 35.0%
AGTTTCGGAAAAGTGAAAGA+TGG + chr4_3:67688179-67688198 Msa0657790:intron 35.0%
GTTTCGGAAAAGTGAAAGAT+GGG + chr4_3:67688180-67688199 Msa0657790:intron 35.0%
GGATTTCAGAGCTATTATCA+AGG - chr4_3:67688215-67688234 None:intergenic 35.0%
! ATGATTCAGTTATGGACTGT+TGG + chr4_3:67688300-67688319 Msa0657790:intron 35.0%
GATAAACATCGCCAAATGTT+AGG - chr4_3:67688473-67688492 None:intergenic 35.0%
! CTTTTGCCACAAACTAAAGT+GGG - chr4_3:67688537-67688556 None:intergenic 35.0%
!! AAAAGGTGCTACAATTGTTG+AGG + chr4_3:67688552-67688571 Msa0657790:intron 35.0%
GTTGCATGCACTACATTATT+AGG + chr4_3:67688615-67688634 Msa0657790:intron 35.0%
CATTATTAGGGATTCCTTTG+TGG + chr4_3:67688628-67688647 Msa0657790:intron 35.0%
! GGTCTTTGATCTTACTGTTT+TGG + chr4_3:67688698-67688717 Msa0657790:intron 35.0%
!! GGTGTTTTTGAACATTATGC+TGG + chr4_3:67688775-67688794 Msa0657790:intron 35.0%
TATGCTGGTGAAGTTATCAT+TGG + chr4_3:67688790-67688809 Msa0657790:intron 35.0%
CAGTTAAGGATTGACATTAG+GGG + chr4_3:67688819-67688838 Msa0657790:intron 35.0%
ACAGTGAAGGAGTTTCAATA+CGG + chr4_3:67688936-67688955 Msa0657790:intron 35.0%
CCAAAGACAACTTTACCATT+TGG - chr4_3:67688993-67689012 None:intergenic 35.0%
CCAAATGGTAAAGTTGTCTT+TGG + chr4_3:67688990-67689009 Msa0657790:intron 35.0%
AATGGTAAAGTTGTCTTTGG+TGG + chr4_3:67688993-67689012 Msa0657790:intron 35.0%
CACTAGAAGTCAAGTCAAAA+AGG + chr4_3:67689082-67689101 Msa0657790:intron 35.0%
CTTCTTCCTAAACCAAGAAT+TGG + chr4_3:67689144-67689163 Msa0657790:intron 35.0%
TCACTTCCAATTCTTGGTTT+AGG - chr4_3:67689153-67689172 None:intergenic 35.0%
TTGTCATCACTTCCAATTCT+TGG - chr4_3:67689159-67689178 None:intergenic 35.0%
! ACCTAGACGGTTTGTTTATT+AGG + chr4_3:67689247-67689266 Msa0657790:intron 35.0%
! CCTAGACGGTTTGTTTATTA+GGG + chr4_3:67689248-67689267 Msa0657790:intron 35.0%
AAACATATACTGATGGAGTG+AGG + chr4_3:67689307-67689326 Msa0657790:intron 35.0%
AACATATACTGATGGAGTGA+GGG + chr4_3:67689308-67689327 Msa0657790:intron 35.0%
ACAGTAGAAGAAAAAGGGTT+AGG + chr4_3:67689340-67689359 Msa0657790:intron 35.0%
ACGTTACTCATAGAATCTTC+AGG - chr4_3:67689540-67689559 None:intergenic 35.0%
CTATGAGTAACGTTATTGAC+AGG + chr4_3:67689547-67689566 Msa0657790:CDS 35.0%
TACTTAGGCATTTCAAGATC+CGG - chr4_3:67689576-67689595 None:intergenic 35.0%
AACTTAGGCATTTCAAGATC+CGG - chr4_3:67689647-67689666 None:intergenic 35.0%
TTGTTAGCAATGAAGTTCAG+GGG - chr4_3:67689808-67689827 None:intergenic 35.0%
CTTGTTAGCAATGAAGTTCA+GGG - chr4_3:67689809-67689828 None:intergenic 35.0%
! ACTTGTTAGCAATGAAGTTC+AGG - chr4_3:67689810-67689829 None:intergenic 35.0%
!! AGAAGACATGTTATTTCAGG+AGG + chr4_3:67689847-67689866 Msa0657790:three_prime_UTR 35.0%
GTTAAGGGAACGAAGATCAT+CGG - chr4_3:67687634-67687653 None:intergenic 40.0%
TGTAGCTCATAATCTGGCTA+AGG - chr4_3:67688121-67688140 None:intergenic 40.0%
!!! GTTATTTTGGATCGTGCAGA+GGG - chr4_3:67688371-67688390 None:intergenic 40.0%
! CCTTTTGCCACAAACTAAAG+TGG - chr4_3:67688538-67688557 None:intergenic 40.0%
!! CCACTTTAGTTTGTGGCAAA+AGG + chr4_3:67688535-67688554 Msa0657790:intron 40.0%
!!! GCTGAAATTTTTCTGGAACC+TGG - chr4_3:67688578-67688597 None:intergenic 40.0%
ACACTAGAAACCAAGCCAAA+TGG + chr4_3:67688975-67688994 Msa0657790:intron 40.0%
GACAACTTTACCATTTGGCT+TGG - chr4_3:67688988-67689007 None:intergenic 40.0%
CTAGTGTTTACATGCTTCTC+TGG - chr4_3:67689068-67689087 None:intergenic 40.0%
CCCTAATAAACAAACCGTCT+AGG - chr4_3:67689251-67689270 None:intergenic 40.0%
ACCATTGTACCTAACCAGAA+CGG - chr4_3:67689283-67689302 None:intergenic 40.0%
! TCATGTTTCTGCAAAAGGCA+AGG - chr4_3:67689482-67689501 None:intergenic 40.0%
! GGTCTTCATGTTTCTGCAAA+AGG - chr4_3:67689487-67689506 None:intergenic 40.0%
GAATCTTCAGGAAATGCATC+TGG - chr4_3:67689528-67689547 None:intergenic 40.0%
TAACGTTATTGACAGGAGAC+CGG + chr4_3:67689554-67689573 Msa0657790:CDS 40.0%
GTTATTCGAACGTGTTTGAC+AGG + chr4_3:67689618-67689637 Msa0657790:three_prime_UTR 40.0%
GTTAAGGGAACGAAGATCAT+CGG - chr4_3:67687634-67687653 None:intergenic 40.0%
TGTAGCTCATAATCTGGCTA+AGG - chr4_3:67688121-67688140 None:intergenic 40.0%
!!! GTTATTTTGGATCGTGCAGA+GGG - chr4_3:67688371-67688390 None:intergenic 40.0%
! CCTTTTGCCACAAACTAAAG+TGG - chr4_3:67688538-67688557 None:intergenic 40.0%
!! CCACTTTAGTTTGTGGCAAA+AGG + chr4_3:67688535-67688554 Msa0657790:intron 40.0%
!!! GCTGAAATTTTTCTGGAACC+TGG - chr4_3:67688578-67688597 None:intergenic 40.0%
ACACTAGAAACCAAGCCAAA+TGG + chr4_3:67688975-67688994 Msa0657790:intron 40.0%
GACAACTTTACCATTTGGCT+TGG - chr4_3:67688988-67689007 None:intergenic 40.0%
CTAGTGTTTACATGCTTCTC+TGG - chr4_3:67689068-67689087 None:intergenic 40.0%
CCCTAATAAACAAACCGTCT+AGG - chr4_3:67689251-67689270 None:intergenic 40.0%
ACCATTGTACCTAACCAGAA+CGG - chr4_3:67689283-67689302 None:intergenic 40.0%
! TCATGTTTCTGCAAAAGGCA+AGG - chr4_3:67689482-67689501 None:intergenic 40.0%
! GGTCTTCATGTTTCTGCAAA+AGG - chr4_3:67689487-67689506 None:intergenic 40.0%
GAATCTTCAGGAAATGCATC+TGG - chr4_3:67689528-67689547 None:intergenic 40.0%
TAACGTTATTGACAGGAGAC+CGG + chr4_3:67689554-67689573 Msa0657790:CDS 40.0%
GTTATTCGAACGTGTTTGAC+AGG + chr4_3:67689618-67689637 Msa0657790:three_prime_UTR 40.0%
TTTCAGATTGGGAAGGTTGC+AGG + chr4_3:67687770-67687789 Msa0657790:intron 45.0%
TACCCAGATTTCTCCTGACA+TGG + chr4_3:67687842-67687861 Msa0657790:CDS 45.0%
AGCCATGTCAGGAGAAATCT+GGG - chr4_3:67687847-67687866 None:intergenic 45.0%
TAGCCATGTCAGGAGAAATC+TGG - chr4_3:67687848-67687867 None:intergenic 45.0%
!!! GGTTATTTTGGATCGTGCAG+AGG - chr4_3:67688372-67688391 None:intergenic 45.0%
!! ACTTCGCCCACTTTAGTTTG+TGG + chr4_3:67688528-67688547 Msa0657790:intron 45.0%
TCAGCAACTTCTGCAAGTTG+AGG + chr4_3:67688593-67688612 Msa0657790:intron 45.0%
GGATTCCTTTGTGGTTCATG+CGG + chr4_3:67688637-67688656 Msa0657790:intron 45.0%
GATTCCTTTGTGGTTCATGC+GGG + chr4_3:67688638-67688657 Msa0657790:intron 45.0%
ATTCCTTTGTGGTTCATGCG+GGG + chr4_3:67688639-67688658 Msa0657790:intron 45.0%
TTGACATTAGGGGCAACAAC+AGG + chr4_3:67688829-67688848 Msa0657790:intron 45.0%
TGACATTAGGGGCAACAACA+GGG + chr4_3:67688830-67688849 Msa0657790:intron 45.0%
CTTGCAAACACAGCAGCAAA+AGG + chr4_3:67689110-67689129 Msa0657790:intron 45.0%
! CTATCTTACCCGTTCTGGTT+AGG + chr4_3:67689271-67689290 Msa0657790:intron 45.0%
CCATTGTACCTAACCAGAAC+GGG - chr4_3:67689282-67689301 None:intergenic 45.0%
GAAGTTCAGGGGCAAGAAAT+TGG - chr4_3:67689797-67689816 None:intergenic 45.0%
TTTCAGATTGGGAAGGTTGC+AGG + chr4_3:67687770-67687789 Msa0657790:intron 45.0%
TACCCAGATTTCTCCTGACA+TGG + chr4_3:67687842-67687861 Msa0657790:CDS 45.0%
AGCCATGTCAGGAGAAATCT+GGG - chr4_3:67687847-67687866 None:intergenic 45.0%
TAGCCATGTCAGGAGAAATC+TGG - chr4_3:67687848-67687867 None:intergenic 45.0%
!!! GGTTATTTTGGATCGTGCAG+AGG - chr4_3:67688372-67688391 None:intergenic 45.0%
!! ACTTCGCCCACTTTAGTTTG+TGG + chr4_3:67688528-67688547 Msa0657790:intron 45.0%
TCAGCAACTTCTGCAAGTTG+AGG + chr4_3:67688593-67688612 Msa0657790:intron 45.0%
GGATTCCTTTGTGGTTCATG+CGG + chr4_3:67688637-67688656 Msa0657790:intron 45.0%
GATTCCTTTGTGGTTCATGC+GGG + chr4_3:67688638-67688657 Msa0657790:intron 45.0%
ATTCCTTTGTGGTTCATGCG+GGG + chr4_3:67688639-67688658 Msa0657790:intron 45.0%
TTGACATTAGGGGCAACAAC+AGG + chr4_3:67688829-67688848 Msa0657790:intron 45.0%
TGACATTAGGGGCAACAACA+GGG + chr4_3:67688830-67688849 Msa0657790:intron 45.0%
CTTGCAAACACAGCAGCAAA+AGG + chr4_3:67689110-67689129 Msa0657790:intron 45.0%
! CTATCTTACCCGTTCTGGTT+AGG + chr4_3:67689271-67689290 Msa0657790:intron 45.0%
CCATTGTACCTAACCAGAAC+GGG - chr4_3:67689282-67689301 None:intergenic 45.0%
GAAGTTCAGGGGCAAGAAAT+TGG - chr4_3:67689797-67689816 None:intergenic 45.0%
!! CGTGTGTTTCTAGCCATGTC+AGG - chr4_3:67687858-67687877 None:intergenic 50.0%
TACTGACCTCCCTCAAGAGT+TGG + chr4_3:67688325-67688344 Msa0657790:intron 50.0%
ACTGACCTCCCTCAAGAGTT+GGG + chr4_3:67688326-67688345 Msa0657790:intron 50.0%
GTGCTACAATTGTTGAGGCC+AGG + chr4_3:67688557-67688576 Msa0657790:intron 50.0%
TTACCCCGCATGAACCACAA+AGG - chr4_3:67688645-67688664 None:intergenic 50.0%
GACATTAGGGGCAACAACAG+GGG + chr4_3:67688831-67688850 Msa0657790:intron 50.0%
TAGGGCTATCTTACCCGTTC+TGG + chr4_3:67689266-67689285 Msa0657790:intron 50.0%
! CCCGTTCTGGTTAGGTACAA+TGG + chr4_3:67689279-67689298 Msa0657790:intron 50.0%
GAACGTGTTTGACAGGAGAC+CGG + chr4_3:67689625-67689644 Msa0657790:three_prime_UTR 50.0%
!! CGTGTGTTTCTAGCCATGTC+AGG - chr4_3:67687858-67687877 None:intergenic 50.0%
TACTGACCTCCCTCAAGAGT+TGG + chr4_3:67688325-67688344 Msa0657790:intron 50.0%
ACTGACCTCCCTCAAGAGTT+GGG + chr4_3:67688326-67688345 Msa0657790:intron 50.0%
GTGCTACAATTGTTGAGGCC+AGG + chr4_3:67688557-67688576 Msa0657790:intron 50.0%
TTACCCCGCATGAACCACAA+AGG - chr4_3:67688645-67688664 None:intergenic 50.0%
GACATTAGGGGCAACAACAG+GGG + chr4_3:67688831-67688850 Msa0657790:intron 50.0%
TAGGGCTATCTTACCCGTTC+TGG + chr4_3:67689266-67689285 Msa0657790:intron 50.0%
! CCCGTTCTGGTTAGGTACAA+TGG + chr4_3:67689279-67689298 Msa0657790:intron 50.0%
GAACGTGTTTGACAGGAGAC+CGG + chr4_3:67689625-67689644 Msa0657790:three_prime_UTR 50.0%
GACCTCCCTCAAGAGTTGGG+AGG + chr4_3:67688329-67688348 Msa0657790:intron 60.0%
TGCCTCCCAACTCTTGAGGG+AGG - chr4_3:67688334-67688353 None:intergenic 60.0%
TCCCTCAAGAGTTGGGAGGC+AGG + chr4_3:67688333-67688352 Msa0657790:intron 60.0%
GCCTGCCTCCCAACTCTTGA+GGG - chr4_3:67688337-67688356 None:intergenic 60.0%
TGCCTGCCTCCCAACTCTTG+AGG - chr4_3:67688338-67688357 None:intergenic 60.0%
GACCTCCCTCAAGAGTTGGG+AGG + chr4_3:67688329-67688348 Msa0657790:intron 60.0%
TGCCTCCCAACTCTTGAGGG+AGG - chr4_3:67688334-67688353 None:intergenic 60.0%
TCCCTCAAGAGTTGGGAGGC+AGG + chr4_3:67688333-67688352 Msa0657790:intron 60.0%
GCCTGCCTCCCAACTCTTGA+GGG - chr4_3:67688337-67688356 None:intergenic 60.0%
TGCCTGCCTCCCAACTCTTG+AGG - chr4_3:67688338-67688357 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 67687515 67689876 67687515 ID=Msa0657790;Name=Msa0657790
chr4_3 mRNA 67687515 67689876 67687515 ID=Msa0657790-mRNA-1;Parent=Msa0657790;Name=Msa0657790-mRNA-1;_AED=0.08;_eAED=0.08;_QI=34|1|1|1|1|1|3|280|125
chr4_3 exon 67687515 67687670 67687515 ID=Msa0657790-mRNA-1:exon:19520;Parent=Msa0657790-mRNA-1
chr4_3 exon 67687776 67687928 67687776 ID=Msa0657790-mRNA-1:exon:19521;Parent=Msa0657790-mRNA-1
chr4_3 exon 67689494 67689876 67689494 ID=Msa0657790-mRNA-1:exon:19522;Parent=Msa0657790-mRNA-1
chr4_3 five_prime_UTR 67687515 67687548 67687515 ID=Msa0657790-mRNA-1:five_prime_utr;Parent=Msa0657790-mRNA-1
chr4_3 CDS 67687549 67687670 67687549 ID=Msa0657790-mRNA-1:cds;Parent=Msa0657790-mRNA-1
chr4_3 CDS 67687776 67687928 67687776 ID=Msa0657790-mRNA-1:cds;Parent=Msa0657790-mRNA-1
chr4_3 CDS 67689494 67689596 67689494 ID=Msa0657790-mRNA-1:cds;Parent=Msa0657790-mRNA-1
chr4_3 three_prime_UTR 67689597 67689876 67689597 ID=Msa0657790-mRNA-1:three_prime_utr;Parent=Msa0657790-mRNA-1
Gene Sequence

>Msa0657790

ATGTCAGAGAAAGAATCAGAATCAGAATCGATGAAATTAGGTTCAGAAGAGCTTTCGAGTGAGTTCAAAACTCTCGTTAGTTCCGATGATCTTCGTTCCCTTAACCATTTACAACACACTATATTGGGAAGGTTGCAGGATAGTAATGCAGTGTTATCACATTTCAATGATTTCTCTCAACATTGTTTTACCCAGATTTCTCCTGACATGGCTAGAAACACACGTGTCTTGAAATCTATCAAATCTGATCTTGATTATATTTTTCTTAAACTTAGAAACATGAAGACCAAACTTTCAACAACTTATCCAGATGCATTTCCTGAAGATTCTATGAGTAACGTTATTGACAGGAGACCGGATCTTGAAATGCCTAAGTAA
Protein sequence

>Msa0657790

MSEKESESESMKLGSEELSSEFKTLVSSDDLRSLNHLQHTILGRLQDSNAVLSHFNDFSQHCFTQISPDMARNTRVLKSIKSDLDYIFLKLRNMKTKLSTTYPDAFPEDSMSNVIDRRPDLEMPK*