AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0710060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0710060 MtrunA17_Chr7g0242761 47.727 88 46 0 20 107 28 115 9.79e-22 82.8
Msa0710060 MtrunA17_Chr2g0289581 42.188 64 37 0 21 84 63 126 2.13e-15 66.6
Msa0710060 MtrunA17_Chr7g0256881 34.211 76 50 0 19 94 1364 1439 8.76e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACAGTTCTTTGCAATTTC+AGG 0.167918 5_1:+1680532 Msa0710070:CDS
AATGCCTTGGTTGTCCCTTT+TGG 0.209134 5_1:+1681074 Msa0710070:intron
CATGTGCATTTAAAACTTTA+TGG 0.214546 5_1:-1681132 Msa0710060:five_prime_UTR
TTGAAAGTTCATCACAAATA+TGG 0.217680 5_1:-1679657 Msa0710060:CDS
CTATTAACTTATACTCGATA+TGG 0.268022 5_1:+1680753 Msa0710070:intron
ATATCATTGCATTTATTTGC+TGG 0.280805 5_1:-1681224 Msa0710060:five_prime_UTR
TTGAAGTTGTGTTGGGATAC+TGG 0.302239 5_1:-1679741 Msa0710060:CDS
GATGAACTTTCAAATCCTTT+TGG 0.313398 5_1:+1679667 None:intergenic
CATATCGAGTATAAGTTAAT+AGG 0.317905 5_1:-1680752 Msa0710060:five_prime_UTR
AACACCTTCTTCGTAGAGAT+TGG 0.323022 5_1:-1679614 Msa0710060:CDS
GTTCTTTGCAATTTCAGGTT+TGG 0.333054 5_1:+1680537 Msa0710070:intron
TCAGCTTCATTCCTCAATAT+AGG 0.376022 5_1:+1680575 Msa0710070:intron
TTTAAAACTTTATGGCAAAC+AGG 0.393954 5_1:-1681124 Msa0710060:five_prime_UTR
ACTTCAATTTATAATCCAAA+AGG 0.425170 5_1:-1681089 Msa0710060:five_prime_UTR
GATATGGATTTGAATGTTTG+AGG 0.435646 5_1:+1680769 Msa0710070:intron
ACACCTTCTTCGTAGAGATT+GGG 0.438915 5_1:-1679613 Msa0710060:CDS
CAACATCCAAAGCGTAATAC+TGG 0.456119 5_1:+1680963 Msa0710070:intron
GGAATGAAGCTGAGAAAGAA+AGG 0.462259 5_1:-1680565 Msa0710060:CDS
CATACACCAGTATTACGCTT+TGG 0.475893 5_1:-1680969 Msa0710060:five_prime_UTR
TGTTGTTGACCTCATCCAAA+AGG 0.478646 5_1:-1679682 Msa0710060:CDS
CTTCAATTTATAATCCAAAA+GGG 0.481209 5_1:-1681088 Msa0710060:five_prime_UTR
GCCATGGTTTGAAGTTGTGT+TGG 0.486369 5_1:-1679749 Msa0710060:CDS
GGATAGAGGTCATGTCAGGA+GGG 0.489390 5_1:+1679486 None:intergenic
CCATGGTTTGAAGTTGTGTT+GGG 0.492443 5_1:-1679748 Msa0710060:CDS
AGGATAGAGGTCATGTCAGG+AGG 0.517326 5_1:+1679485 None:intergenic
ATATGGATTTGAATGTTTGA+GGG 0.520629 5_1:+1680770 Msa0710070:intron
AGAGAATATCACCTATATTG+AGG 0.521853 5_1:-1680586 Msa0710060:five_prime_UTR
GAGTAGTTTGAAATCAATGA+GGG 0.530642 5_1:+1680937 Msa0710070:intron
ATTAAGATACAGTAGTGAGA+GGG 0.533333 5_1:+1681250 Msa0710070:intron
CCTCCCAATCTCTACGAAGA+AGG 0.543112 5_1:+1679610 None:intergenic
TTACGATATACTTTGTGTGA+GGG 0.550768 5_1:-1679582 Msa0710060:CDS
GAATGTTTGAGGGAAGAAAG+AGG 0.559677 5_1:+1680780 Msa0710070:intron
GATAGAGGTCATGTCAGGAG+GGG 0.563700 5_1:+1679487 None:intergenic
TTTACGATATACTTTGTGTG+AGG 0.568426 5_1:-1679583 Msa0710060:CDS
GATTAAGATACAGTAGTGAG+AGG 0.572514 5_1:+1681249 Msa0710070:intron
ATAGAGGTCATGTCAGGAGG+GGG 0.585310 5_1:+1679488 None:intergenic
CCTTCTTCGTAGAGATTGGG+AGG 0.587851 5_1:-1679610 Msa0710060:CDS
GGAGTAGTTTGAAATCAATG+AGG 0.591995 5_1:+1680936 Msa0710070:intron
CAAATATGGAAATTTAGTCA+TGG 0.604618 5_1:-1679643 Msa0710060:CDS
TTAAGATACAGTAGTGAGAG+GGG 0.628639 5_1:+1681251 Msa0710070:intron
CCCAACACAACTTCAAACCA+TGG 0.660552 5_1:+1679748 None:intergenic
GGATACTGGATTTAAGCATG+TGG 0.673744 5_1:-1679727 Msa0710060:CDS
TAATCCAAAAGGGACAACCA+AGG 0.685647 5_1:-1681078 Msa0710060:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTTATATATTTTTTT+GGG + chr5_1:1680968-1680987 Msa0710070:intron 0.0%
!!! TTTTAATTTATATATTTTTT+TGG + chr5_1:1680969-1680988 Msa0710070:intron 0.0%
!!! TTTAATTTATATATTTTTTT+GGG + chr5_1:1680968-1680987 Msa0710070:intron 0.0%
!!! TTTTAATTTATATATTTTTT+TGG + chr5_1:1680969-1680988 Msa0710070:intron 0.0%
!!! TTTTTTTTTTTTGTTATAGA+TGG - chr5_1:1680631-1680650 Msa0710060:five_prime_UTR 10.0%
!! AATAAGTTAAAATATATTAC+AGG + chr5_1:1680697-1680716 Msa0710070:intron 10.0%
!!! ATTTTAATCAAAAAAAATTG+TGG + chr5_1:1680852-1680871 Msa0710070:intron 10.0%
!!! TTTTTTTTTTTTGTTATAGA+TGG - chr5_1:1680631-1680650 Msa0710060:five_prime_UTR 10.0%
!! AATAAGTTAAAATATATTAC+AGG + chr5_1:1680697-1680716 Msa0710070:intron 10.0%
!!! ATTTTAATCAAAAAAAATTG+TGG + chr5_1:1680852-1680871 Msa0710070:intron 10.0%
!!! TACAACATTTTTTTTATGAA+GGG + chr5_1:1679939-1679958 None:intergenic 15.0%
!!! TTTGTTGAAATTTTGATTTT+TGG + chr5_1:1680189-1680208 None:intergenic 15.0%
!!! TTTTTTGATTTTGATGAAAT+GGG + chr5_1:1680212-1680231 None:intergenic 15.0%
!!! ATTTTTTGATTTTGATGAAA+TGG + chr5_1:1680213-1680232 None:intergenic 15.0%
!!! ATTTTTTTGATGAATAATGT+GGG + chr5_1:1680812-1680831 Msa0710070:intron 15.0%
!!! TATTTTTTTGATGAATAATG+TGG + chr5_1:1680813-1680832 Msa0710070:intron 15.0%
!!! ATTTATATATTTTTTTGGGA+CGG + chr5_1:1680964-1680983 Msa0710070:intron 15.0%
!!! TACAACATTTTTTTTATGAA+GGG + chr5_1:1679939-1679958 None:intergenic 15.0%
!!! TTTGTTGAAATTTTGATTTT+TGG + chr5_1:1680189-1680208 None:intergenic 15.0%
!!! TTTTTTGATTTTGATGAAAT+GGG + chr5_1:1680212-1680231 None:intergenic 15.0%
!!! ATTTTTTGATTTTGATGAAA+TGG + chr5_1:1680213-1680232 None:intergenic 15.0%
!!! ATTTTTTTGATGAATAATGT+GGG + chr5_1:1680812-1680831 Msa0710070:intron 15.0%
!!! TATTTTTTTGATGAATAATG+TGG + chr5_1:1680813-1680832 Msa0710070:intron 15.0%
!!! ATTTATATATTTTTTTGGGA+CGG + chr5_1:1680964-1680983 Msa0710070:intron 15.0%
!!! ACATTTTTTCTTTTGTTCAT+TGG - chr5_1:1679531-1679550 Msa0710060:CDS 20.0%
!!! ACATTTTAGCTTAAAATTAG+TGG - chr5_1:1679679-1679698 Msa0710060:CDS 20.0%
!! ACTTCAATTTATAATCCAAA+AGG - chr5_1:1679762-1679781 Msa0710060:intron 20.0%
!! CTTCAATTTATAATCCAAAA+GGG - chr5_1:1679763-1679782 Msa0710060:intron 20.0%
!!! GTATTTTTGTTTTCATGTAA+AGG + chr5_1:1679826-1679845 None:intergenic 20.0%
!!! GTACAACATTTTTTTTATGA+AGG + chr5_1:1679940-1679959 None:intergenic 20.0%
!!! TAATTTCAAGCATTTTTTTG+TGG - chr5_1:1680015-1680034 Msa0710060:intron 20.0%
!! GTTAAAATATATTACAGGAT+CGG + chr5_1:1680692-1680711 Msa0710070:intron 20.0%
!!! ACATTTTTTCTTTTGTTCAT+TGG - chr5_1:1679531-1679550 Msa0710060:CDS 20.0%
!!! ACATTTTAGCTTAAAATTAG+TGG - chr5_1:1679679-1679698 Msa0710060:CDS 20.0%
!! ACTTCAATTTATAATCCAAA+AGG - chr5_1:1679762-1679781 Msa0710060:intron 20.0%
!! CTTCAATTTATAATCCAAAA+GGG - chr5_1:1679763-1679782 Msa0710060:intron 20.0%
!!! GTATTTTTGTTTTCATGTAA+AGG + chr5_1:1679826-1679845 None:intergenic 20.0%
!!! GTACAACATTTTTTTTATGA+AGG + chr5_1:1679940-1679959 None:intergenic 20.0%
!!! TAATTTCAAGCATTTTTTTG+TGG - chr5_1:1680015-1680034 Msa0710060:intron 20.0%
!! GTTAAAATATATTACAGGAT+CGG + chr5_1:1680692-1680711 Msa0710070:intron 20.0%
!! CTTTTATGATAGCTAAAAAG+TGG + chr5_1:1679505-1679524 None:intergenic 25.0%
! ATATCATTGCATTTATTTGC+TGG - chr5_1:1679627-1679646 Msa0710060:CDS 25.0%
!! CATGTGCATTTAAAACTTTA+TGG - chr5_1:1679719-1679738 Msa0710060:CDS 25.0%
!! TTTAAAACTTTATGGCAAAC+AGG - chr5_1:1679727-1679746 Msa0710060:CDS 25.0%
!! TAATACTGGTGTATGATTTT+AGG + chr5_1:1679877-1679896 None:intergenic 25.0%
! ATATGGATTTGAATGTTTGA+GGG + chr5_1:1680084-1680103 None:intergenic 25.0%
! CTATTAACTTATACTCGATA+TGG + chr5_1:1680101-1680120 None:intergenic 25.0%
! CATATCGAGTATAAGTTAAT+AGG - chr5_1:1680099-1680118 Msa0710060:intron 25.0%
!! TGCATTTTCTGTCAAATTAA+AGG - chr5_1:1680127-1680146 Msa0710060:intron 25.0%
!!! AGAGTTTGTATTTTTGTTTC+TGG + chr5_1:1680162-1680181 None:intergenic 25.0%
! AAATTGTGGAAATTGTTAGA+GGG + chr5_1:1680838-1680857 Msa0710070:intron 25.0%
! AAAATTGTGGAAATTGTTAG+AGG + chr5_1:1680839-1680858 Msa0710070:intron 25.0%
!!! AATATTTTTGTCGCGAATTT+GGG + chr5_1:1680907-1680926 Msa0710070:intron 25.0%
!!! TAATATTTTTGTCGCGAATT+TGG + chr5_1:1680908-1680927 Msa0710070:intron 25.0%
! AGAAAACAGTCAAATTAAGA+CGG + chr5_1:1681076-1681095 Msa0710070:intron 25.0%
! TTGAAAGTTCATCACAAATA+TGG - chr5_1:1681194-1681213 Msa0710060:five_prime_UTR 25.0%
! CAAATATGGAAATTTAGTCA+TGG - chr5_1:1681208-1681227 Msa0710060:five_prime_UTR 25.0%
!!! AACTGATTTTCTAGCTAAAA+AGG - chr5_1:1681301-1681320 Msa0710060:five_prime_UTR 25.0%
!!! ACTGATTTTCTAGCTAAAAA+GGG - chr5_1:1681302-1681321 Msa0710060:five_prime_UTR 25.0%
!! CTTTTATGATAGCTAAAAAG+TGG + chr5_1:1679505-1679524 None:intergenic 25.0%
! ATATCATTGCATTTATTTGC+TGG - chr5_1:1679627-1679646 Msa0710060:CDS 25.0%
!! CATGTGCATTTAAAACTTTA+TGG - chr5_1:1679719-1679738 Msa0710060:CDS 25.0%
!! TTTAAAACTTTATGGCAAAC+AGG - chr5_1:1679727-1679746 Msa0710060:CDS 25.0%
!! TAATACTGGTGTATGATTTT+AGG + chr5_1:1679877-1679896 None:intergenic 25.0%
! ATATGGATTTGAATGTTTGA+GGG + chr5_1:1680084-1680103 None:intergenic 25.0%
! CTATTAACTTATACTCGATA+TGG + chr5_1:1680101-1680120 None:intergenic 25.0%
! CATATCGAGTATAAGTTAAT+AGG - chr5_1:1680099-1680118 Msa0710060:intron 25.0%
!! TGCATTTTCTGTCAAATTAA+AGG - chr5_1:1680127-1680146 Msa0710060:intron 25.0%
!!! AGAGTTTGTATTTTTGTTTC+TGG + chr5_1:1680162-1680181 None:intergenic 25.0%
! AAATTGTGGAAATTGTTAGA+GGG + chr5_1:1680838-1680857 Msa0710070:intron 25.0%
! AAAATTGTGGAAATTGTTAG+AGG + chr5_1:1680839-1680858 Msa0710070:intron 25.0%
!!! AATATTTTTGTCGCGAATTT+GGG + chr5_1:1680907-1680926 Msa0710070:intron 25.0%
!!! TAATATTTTTGTCGCGAATT+TGG + chr5_1:1680908-1680927 Msa0710070:intron 25.0%
! AGAAAACAGTCAAATTAAGA+CGG + chr5_1:1681076-1681095 Msa0710070:intron 25.0%
! TTGAAAGTTCATCACAAATA+TGG - chr5_1:1681194-1681213 Msa0710060:five_prime_UTR 25.0%
! CAAATATGGAAATTTAGTCA+TGG - chr5_1:1681208-1681227 Msa0710060:five_prime_UTR 25.0%
!!! AACTGATTTTCTAGCTAAAA+AGG - chr5_1:1681301-1681320 Msa0710060:five_prime_UTR 25.0%
!!! ACTGATTTTCTAGCTAAAAA+GGG - chr5_1:1681302-1681321 Msa0710060:five_prime_UTR 25.0%
ATTAAGATACAGTAGTGAGA+GGG + chr5_1:1679604-1679623 None:intergenic 30.0%
! GAGTAGTTTGAAATCAATGA+GGG + chr5_1:1679917-1679936 None:intergenic 30.0%
GATATGGATTTGAATGTTTG+AGG + chr5_1:1680085-1680104 None:intergenic 30.0%
AGAGAATATCACCTATATTG+AGG - chr5_1:1680265-1680284 Msa0710060:intron 30.0%
TGAGAGATGAAATTTATGGA+GGG - chr5_1:1680476-1680495 Msa0710060:intron 30.0%
! TTTTTGATGAATAATGTGGG+CGG + chr5_1:1680809-1680828 Msa0710070:intron 30.0%
TAAAATAGACCAAGTTGTGA+CGG - chr5_1:1680866-1680885 Msa0710060:five_prime_UTR 30.0%
!! TTTTTATTTCCGTCACAACT+TGG + chr5_1:1680878-1680897 Msa0710070:intron 30.0%
! AATATTTCTGTCGCGAATAT+GGG + chr5_1:1680938-1680957 Msa0710070:intron 30.0%
! TAATATTTCTGTCGCGAATA+TGG + chr5_1:1680939-1680958 Msa0710070:intron 30.0%
! GATGAACTTTCAAATCCTTT+TGG + chr5_1:1681187-1681206 Msa0710070:intron 30.0%
TTTACGATATACTTTGTGTG+AGG - chr5_1:1681268-1681287 Msa0710060:five_prime_UTR 30.0%
TTACGATATACTTTGTGTGA+GGG - chr5_1:1681269-1681288 Msa0710060:five_prime_UTR 30.0%
ATTAAGATACAGTAGTGAGA+GGG + chr5_1:1679604-1679623 None:intergenic 30.0%
! GAGTAGTTTGAAATCAATGA+GGG + chr5_1:1679917-1679936 None:intergenic 30.0%
GATATGGATTTGAATGTTTG+AGG + chr5_1:1680085-1680104 None:intergenic 30.0%
AGAGAATATCACCTATATTG+AGG - chr5_1:1680265-1680284 Msa0710060:intron 30.0%
TGAGAGATGAAATTTATGGA+GGG - chr5_1:1680476-1680495 Msa0710060:intron 30.0%
! TTTTTGATGAATAATGTGGG+CGG + chr5_1:1680809-1680828 Msa0710070:intron 30.0%
TAAAATAGACCAAGTTGTGA+CGG - chr5_1:1680866-1680885 Msa0710060:five_prime_UTR 30.0%
!! TTTTTATTTCCGTCACAACT+TGG + chr5_1:1680878-1680897 Msa0710070:intron 30.0%
! AATATTTCTGTCGCGAATAT+GGG + chr5_1:1680938-1680957 Msa0710070:intron 30.0%
! TAATATTTCTGTCGCGAATA+TGG + chr5_1:1680939-1680958 Msa0710070:intron 30.0%
! GATGAACTTTCAAATCCTTT+TGG + chr5_1:1681187-1681206 Msa0710070:intron 30.0%
TTTACGATATACTTTGTGTG+AGG - chr5_1:1681268-1681287 Msa0710060:five_prime_UTR 30.0%
TTACGATATACTTTGTGTGA+GGG - chr5_1:1681269-1681288 Msa0710060:five_prime_UTR 30.0%
TTAAGATACAGTAGTGAGAG+GGG + chr5_1:1679603-1679622 None:intergenic 35.0%
GATTAAGATACAGTAGTGAG+AGG + chr5_1:1679605-1679624 None:intergenic 35.0%
! GCTTTTCTACAATAATGCCT+TGG + chr5_1:1679793-1679812 None:intergenic 35.0%
! GGAGTAGTTTGAAATCAATG+AGG + chr5_1:1679918-1679937 None:intergenic 35.0%
TCAGCTTCATTCCTCAATAT+AGG + chr5_1:1680279-1680298 None:intergenic 35.0%
!! GTTCTTTGCAATTTCAGGTT+TGG + chr5_1:1680317-1680336 None:intergenic 35.0%
TCACAGTTCTTTGCAATTTC+AGG + chr5_1:1680322-1680341 None:intergenic 35.0%
CGAGTGAGAGATGAAATTTA+TGG - chr5_1:1680472-1680491 Msa0710060:intron 35.0%
GTGAGAGATGAAATTTATGG+AGG - chr5_1:1680475-1680494 Msa0710060:intron 35.0%
ATGAAATTTATGGAGGGTTG+TGG - chr5_1:1680482-1680501 Msa0710060:intron 35.0%
TGAAATTTATGGAGGGTTGT+GGG - chr5_1:1680483-1680502 Msa0710060:intron 35.0%
TTATAGATGGTTGTGTTGTG+TGG - chr5_1:1680644-1680663 Msa0710060:five_prime_UTR 35.0%
! TTTTGATGAATAATGTGGGC+GGG + chr5_1:1680808-1680827 Msa0710070:intron 35.0%
CCAAATTAATTAAAGCGGGA+CGG + chr5_1:1681025-1681044 Msa0710070:intron 35.0%
CGTCCCGCTTTAATTAATTT+GGG - chr5_1:1681023-1681042 Msa0710060:intron 35.0%
CGTCCCAAATTAATTAAAGC+GGG + chr5_1:1681029-1681048 Msa0710070:intron 35.0%
CCGTCCCAAATTAATTAAAG+CGG + chr5_1:1681030-1681049 Msa0710070:intron 35.0%
! CCGCTTTAATTAATTTGGGA+CGG - chr5_1:1681027-1681046 Msa0710060:intron 35.0%
! CTTTCAAATCCTTTTGGATG+AGG + chr5_1:1681181-1681200 Msa0710070:intron 35.0%
TTAAGATACAGTAGTGAGAG+GGG + chr5_1:1679603-1679622 None:intergenic 35.0%
GATTAAGATACAGTAGTGAG+AGG + chr5_1:1679605-1679624 None:intergenic 35.0%
! GCTTTTCTACAATAATGCCT+TGG + chr5_1:1679793-1679812 None:intergenic 35.0%
! GGAGTAGTTTGAAATCAATG+AGG + chr5_1:1679918-1679937 None:intergenic 35.0%
TCAGCTTCATTCCTCAATAT+AGG + chr5_1:1680279-1680298 None:intergenic 35.0%
!! GTTCTTTGCAATTTCAGGTT+TGG + chr5_1:1680317-1680336 None:intergenic 35.0%
TCACAGTTCTTTGCAATTTC+AGG + chr5_1:1680322-1680341 None:intergenic 35.0%
CGAGTGAGAGATGAAATTTA+TGG - chr5_1:1680472-1680491 Msa0710060:intron 35.0%
GTGAGAGATGAAATTTATGG+AGG - chr5_1:1680475-1680494 Msa0710060:intron 35.0%
ATGAAATTTATGGAGGGTTG+TGG - chr5_1:1680482-1680501 Msa0710060:intron 35.0%
TGAAATTTATGGAGGGTTGT+GGG - chr5_1:1680483-1680502 Msa0710060:intron 35.0%
TTATAGATGGTTGTGTTGTG+TGG - chr5_1:1680644-1680663 Msa0710060:five_prime_UTR 35.0%
! TTTTGATGAATAATGTGGGC+GGG + chr5_1:1680808-1680827 Msa0710070:intron 35.0%
CCAAATTAATTAAAGCGGGA+CGG + chr5_1:1681025-1681044 Msa0710070:intron 35.0%
CGTCCCGCTTTAATTAATTT+GGG - chr5_1:1681023-1681042 Msa0710060:intron 35.0%
CGTCCCAAATTAATTAAAGC+GGG + chr5_1:1681029-1681048 Msa0710070:intron 35.0%
CCGTCCCAAATTAATTAAAG+CGG + chr5_1:1681030-1681049 Msa0710070:intron 35.0%
! CCGCTTTAATTAATTTGGGA+CGG - chr5_1:1681027-1681046 Msa0710060:intron 35.0%
! CTTTCAAATCCTTTTGGATG+AGG + chr5_1:1681181-1681200 Msa0710070:intron 35.0%
TAATCCAAAAGGGACAACCA+AGG - chr5_1:1679773-1679792 Msa0710060:intron 40.0%
CATACACCAGTATTACGCTT+TGG - chr5_1:1679882-1679901 Msa0710060:intron 40.0%
CAACATCCAAAGCGTAATAC+TGG + chr5_1:1679891-1679910 None:intergenic 40.0%
GAATGTTTGAGGGAAGAAAG+AGG + chr5_1:1680074-1680093 None:intergenic 40.0%
GGAATGAAGCTGAGAAAGAA+AGG - chr5_1:1680286-1680305 Msa0710060:intron 40.0%
!! AGAGAGAGCGATTTGTTGTT+AGG - chr5_1:1680585-1680604 Msa0710060:five_prime_UTR 40.0%
!! GAGAGAGCGATTTGTTGTTA+GGG - chr5_1:1680586-1680605 Msa0710060:five_prime_UTR 40.0%
GTTGTGTTGTGTGGTGTATA+TGG - chr5_1:1680653-1680672 Msa0710060:five_prime_UTR 40.0%
TGTTGTGTGGTGTATATGGA+TGG - chr5_1:1680657-1680676 Msa0710060:five_prime_UTR 40.0%
TGTGGTGTATATGGATGGAT+TGG - chr5_1:1680662-1680681 Msa0710060:five_prime_UTR 40.0%
! TTTTTGTCGCGAATTTGGGA+CGG + chr5_1:1680903-1680922 Msa0710070:intron 40.0%
CCGTCCCGCTTTAATTAATT+TGG - chr5_1:1681022-1681041 Msa0710060:intron 40.0%
ATTAAGACGGAACATCGTGA+CGG + chr5_1:1681063-1681082 Msa0710070:intron 40.0%
! ACTGTTTTCTTGTAGTGCCA+TGG - chr5_1:1681086-1681105 Msa0710060:five_prime_UTR 40.0%
! CCATGGTTTGAAGTTGTGTT+GGG - chr5_1:1681103-1681122 Msa0710060:five_prime_UTR 40.0%
TTGAAGTTGTGTTGGGATAC+TGG - chr5_1:1681110-1681129 Msa0710060:five_prime_UTR 40.0%
GGATACTGGATTTAAGCATG+TGG - chr5_1:1681124-1681143 Msa0710060:five_prime_UTR 40.0%
TGTTGTTGACCTCATCCAAA+AGG - chr5_1:1681169-1681188 Msa0710060:five_prime_UTR 40.0%
AACACCTTCTTCGTAGAGAT+TGG - chr5_1:1681237-1681256 Msa0710060:five_prime_UTR 40.0%
ACACCTTCTTCGTAGAGATT+GGG - chr5_1:1681238-1681257 Msa0710060:five_prime_UTR 40.0%
TAATCCAAAAGGGACAACCA+AGG - chr5_1:1679773-1679792 Msa0710060:intron 40.0%
CATACACCAGTATTACGCTT+TGG - chr5_1:1679882-1679901 Msa0710060:intron 40.0%
CAACATCCAAAGCGTAATAC+TGG + chr5_1:1679891-1679910 None:intergenic 40.0%
GAATGTTTGAGGGAAGAAAG+AGG + chr5_1:1680074-1680093 None:intergenic 40.0%
GGAATGAAGCTGAGAAAGAA+AGG - chr5_1:1680286-1680305 Msa0710060:intron 40.0%
!! AGAGAGAGCGATTTGTTGTT+AGG - chr5_1:1680585-1680604 Msa0710060:five_prime_UTR 40.0%
!! GAGAGAGCGATTTGTTGTTA+GGG - chr5_1:1680586-1680605 Msa0710060:five_prime_UTR 40.0%
GTTGTGTTGTGTGGTGTATA+TGG - chr5_1:1680653-1680672 Msa0710060:five_prime_UTR 40.0%
TGTTGTGTGGTGTATATGGA+TGG - chr5_1:1680657-1680676 Msa0710060:five_prime_UTR 40.0%
TGTGGTGTATATGGATGGAT+TGG - chr5_1:1680662-1680681 Msa0710060:five_prime_UTR 40.0%
! TTTTTGTCGCGAATTTGGGA+CGG + chr5_1:1680903-1680922 Msa0710070:intron 40.0%
CCGTCCCGCTTTAATTAATT+TGG - chr5_1:1681022-1681041 Msa0710060:intron 40.0%
ATTAAGACGGAACATCGTGA+CGG + chr5_1:1681063-1681082 Msa0710070:intron 40.0%
! ACTGTTTTCTTGTAGTGCCA+TGG - chr5_1:1681086-1681105 Msa0710060:five_prime_UTR 40.0%
! CCATGGTTTGAAGTTGTGTT+GGG - chr5_1:1681103-1681122 Msa0710060:five_prime_UTR 40.0%
TTGAAGTTGTGTTGGGATAC+TGG - chr5_1:1681110-1681129 Msa0710060:five_prime_UTR 40.0%
GGATACTGGATTTAAGCATG+TGG - chr5_1:1681124-1681143 Msa0710060:five_prime_UTR 40.0%
TGTTGTTGACCTCATCCAAA+AGG - chr5_1:1681169-1681188 Msa0710060:five_prime_UTR 40.0%
AACACCTTCTTCGTAGAGAT+TGG - chr5_1:1681237-1681256 Msa0710060:five_prime_UTR 40.0%
ACACCTTCTTCGTAGAGATT+GGG - chr5_1:1681238-1681257 Msa0710060:five_prime_UTR 40.0%
! AATGCCTTGGTTGTCCCTTT+TGG + chr5_1:1679780-1679799 None:intergenic 45.0%
! CTGGTGTATGATTTTAGGCG+TGG + chr5_1:1679872-1679891 None:intergenic 45.0%
! GCTTTGAGTGAGCGATTGAA+AGG - chr5_1:1680548-1680567 Msa0710060:CDS 45.0%
AATAATGTGGGCGGGAAACT+TGG + chr5_1:1680800-1680819 Msa0710070:intron 45.0%
TTTCTGTCGCGAATATGGGA+CGG + chr5_1:1680934-1680953 Msa0710070:intron 45.0%
! GCCATGGTTTGAAGTTGTGT+TGG - chr5_1:1681102-1681121 Msa0710060:five_prime_UTR 45.0%
CCCAACACAACTTCAAACCA+TGG + chr5_1:1681106-1681125 Msa0710070:intron 45.0%
!! GCTAAAAAGGGTGCTCTTAG+TGG - chr5_1:1681314-1681333 Msa0710060:five_prime_UTR 45.0%
! AATGCCTTGGTTGTCCCTTT+TGG + chr5_1:1679780-1679799 None:intergenic 45.0%
! CTGGTGTATGATTTTAGGCG+TGG + chr5_1:1679872-1679891 None:intergenic 45.0%
! GCTTTGAGTGAGCGATTGAA+AGG - chr5_1:1680548-1680567 Msa0710060:CDS 45.0%
AATAATGTGGGCGGGAAACT+TGG + chr5_1:1680800-1680819 Msa0710070:intron 45.0%
TTTCTGTCGCGAATATGGGA+CGG + chr5_1:1680934-1680953 Msa0710070:intron 45.0%
! GCCATGGTTTGAAGTTGTGT+TGG - chr5_1:1681102-1681121 Msa0710060:five_prime_UTR 45.0%
CCCAACACAACTTCAAACCA+TGG + chr5_1:1681106-1681125 Msa0710070:intron 45.0%
!! GCTAAAAAGGGTGCTCTTAG+TGG - chr5_1:1681314-1681333 Msa0710060:five_prime_UTR 45.0%
!!! TATTTTATCTTTTTTTTTTT+AGG - chr5_1:1680713-1680732 Msa0710060:five_prime_UTR 5.0%
!!! ATTTTATCTTTTTTTTTTTA+GGG - chr5_1:1680714-1680733 Msa0710060:five_prime_UTR 5.0%
!! AAAAATTAATTATAATAAAG+TGG - chr5_1:1680772-1680791 Msa0710060:five_prime_UTR 5.0%
!!! TATTTTATCTTTTTTTTTTT+AGG - chr5_1:1680713-1680732 Msa0710060:five_prime_UTR 5.0%
!!! ATTTTATCTTTTTTTTTTTA+GGG - chr5_1:1680714-1680733 Msa0710060:five_prime_UTR 5.0%
!! AAAAATTAATTATAATAAAG+TGG - chr5_1:1680772-1680791 Msa0710060:five_prime_UTR 5.0%
CCTCCCAATCTCTACGAAGA+AGG + chr5_1:1681244-1681263 Msa0710070:intron 50.0%
CCTTCTTCGTAGAGATTGGG+AGG - chr5_1:1681241-1681260 Msa0710060:five_prime_UTR 50.0%
CCTCCCAATCTCTACGAAGA+AGG + chr5_1:1681244-1681263 Msa0710070:intron 50.0%
CCTTCTTCGTAGAGATTGGG+AGG - chr5_1:1681241-1681260 Msa0710060:five_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 1679500 1681373 1679500 ID=Msa0710060;Name=Msa0710060
chr5_1 mRNA 1679500 1681373 1679500 ID=Msa0710060-mRNA-1;Parent=Msa0710060;Name=Msa0710060-mRNA-1;_AED=0.13;_eAED=0.13;_QI=764|0.5|0.33|0.66|0.5|0.33|3|0|108
chr5_1 exon 1679500 1679772 1679500 ID=Msa0710060-mRNA-1:exon:562;Parent=Msa0710060-mRNA-1
chr5_1 exon 1680531 1681027 1680531 ID=Msa0710060-mRNA-1:exon:561;Parent=Msa0710060-mRNA-1
chr5_1 exon 1681053 1681373 1681053 ID=Msa0710060-mRNA-1:exon:560;Parent=Msa0710060-mRNA-1
chr5_1 five_prime_UTR 1681053 1681373 1681053 ID=Msa0710060-mRNA-1:five_prime_utr;Parent=Msa0710060-mRNA-1
chr5_1 five_prime_UTR 1680585 1681027 1680585 ID=Msa0710060-mRNA-1:five_prime_utr;Parent=Msa0710060-mRNA-1
chr5_1 CDS 1680531 1680584 1680531 ID=Msa0710060-mRNA-1:cds;Parent=Msa0710060-mRNA-1
chr5_1 CDS 1679500 1679772 1679500 ID=Msa0710060-mRNA-1:cds;Parent=Msa0710060-mRNA-1
Gene Sequence

>Msa0710060

ATGAAGCTGAGAAAGAAAGGAAATTCCAAACCTGAAATTGCAAAGAACTGTGAATGCCATGGTTTGAAGTTGTGTTGGGATACTGGATTTAAGCATGTGGTGTGTCTCTCTGATTCAACTATTGTTGTTGACCTCATCCAAAAGGATTTGAAAGTTCATCACAAATATGGAAATTTAGTCATGGCTATTAAACACCTTCTTCGTAGAGATTGGGAGGTTAGTTTACGATATACTTTGTGTGAGGGTAACGCTGCAACTGATTTTCTAGCTAAAAAGGGTGCTCTTAGTGGTAGTAGCTTTGTTATTTTGAATGAAGCCCCCTCCTGA
Protein sequence

>Msa0710060

MKLRKKGNSKPEIAKNCECHGLKLCWDTGFKHVVCLSDSTIVVDLIQKDLKVHHKYGNLVMAIKHLLRRDWEVSLRYTLCEGNAATDFLAKKGALSGSSFVILNEAPS*