AlfalfaGEDB | Msa0723340

M. sativa cultivar zhongmu-4 / Msa0723340

KEGG: K15078|K19613
GO: -
PFAM: PF18052.4

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0723340	XP_024639251.2	92.038	314	25	0	1	314	1	314	0.0	591

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0723340	sp\|Q7XA40\|RGA3_SOLBU	50.000	314	141	2	1	314	1	298	3.19e-100	319

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0723340	G7K2V7	92.038	314	25	0	1	314	1	314	0.0	591

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0294950	Msa0723340	0.802413	5.675809e-49	-8.615850e-47
Msa0331920	Msa0723340	0.822416	2.397477e-53	-8.615850e-47
Msa0338110	Msa0723340	0.805682	1.186655e-49	-8.615850e-47
Msa0432300	Msa0723340	0.802172	6.365517e-49	-8.615850e-47
Msa0448550	Msa0723340	-0.808887	2.483274e-50	-8.615850e-47
Msa0492880	Msa0723340	0.808138	3.587701e-50	-8.615850e-47
Msa0498220	Msa0723340	0.803693	3.086653e-49	-8.615850e-47
Msa0626280	Msa0723340	0.807522	4.850382e-50	-8.615850e-47
Msa0658570	Msa0723340	0.810673	1.025489e-50	-8.615850e-47
Msa0723320	Msa0723340	0.810478	1.129737e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0723340	MtrunA17_Chr5g0414231	92.038	314	25	0	1	314	1	314	0.0	591
Msa0723340	MtrunA17_Chr3g0087301	78.662	314	67	0	1	314	1	314	1.21e-178	523
Msa0723340	MtrunA17_Chr5g0415511	80.892	314	60	0	1	314	1	314	2.60e-176	506
Msa0723340	MtrunA17_Chr5g0414201	83.439	314	52	0	1	314	1	314	4.01e-176	512
Msa0723340	MtrunA17_Chr5g0414181	79.299	314	64	1	1	314	1	313	1.53e-175	511
Msa0723340	MtrunA17_Chr5g0414291	74.320	331	79	2	1	325	1	331	3.01e-174	489
Msa0723340	MtrunA17_Chr5g0415471	74.924	331	75	3	1	325	1	329	6.92e-166	491
Msa0723340	MtrunA17_Chr5g0414311	70.662	317	89	1	1	313	1	317	3.35e-151	453
Msa0723340	MtrunA17_Chr5g0413821	77.273	220	50	0	1	220	1	220	2.71e-123	352
Msa0723340	MtrunA17_Chr5g0414171	77.674	215	44	1	1	215	1	211	7.21e-118	346

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0723340	AT3G14470.1	33.670	297	176	6	28	314	36	321	1.10e-42	157
Msa0723340	AT3G14470.2	33.670	297	176	6	28	314	36	321	1.10e-42	157
Msa0723340	AT3G50950.2	31.447	318	203	6	1	316	1	305	4.16e-38	144
Msa0723340	AT3G50950.1	31.447	318	203	6	1	316	1	305	4.16e-38	144
Msa0723340	AT3G14460.1	30.584	291	189	4	31	316	36	318	4.26e-35	135
Msa0723340	AT5G48620.6	29.697	330	201	9	1	318	1	311	1.39e-32	128
Msa0723340	AT5G48620.1	29.697	330	201	9	1	318	1	311	1.39e-32	128
Msa0723340	AT5G48620.4	29.697	330	201	9	1	318	1	311	1.39e-32	128
Msa0723340	AT5G48620.2	29.697	330	201	9	1	318	1	311	1.39e-32	128
Msa0723340	AT5G48620.3	29.697	330	201	9	1	318	1	311	1.39e-32	128

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Find 71 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACACTTTGAGCTAAGAATT+TGG	0.234703	5_1:-20890275	Msa0723340:CDS
AACTTGCTAAGAAAATGAAA+AGG	0.579865	5_1:-20890581	Msa0723340:CDS
AAGCTTGCTCACTACAATGA+AGG	0.544631	5_1:-20890826	Msa0723340:CDS
ACACTTTGAGCTAAGAATTT+GGG	0.367571	5_1:-20890274	Msa0723340:CDS
ACTCGTCCAAGATGTCGTCG+AGG	0.720486	5_1:+20890718	None:intergenic
AGAATTCGGAGGCTCGGCTC+TGG	0.408724	5_1:-20889975	Msa0723340:CDS
AGATCTGTGTTGGCTTGTGG+AGG	0.620989	5_1:-20890060	Msa0723340:CDS
AGCCGAGCCTCCGAATTCTC+AGG	0.422786	5_1:+20889979	None:intergenic
AGCCTCCGAATTCTCAGGGA+CGG	0.567688	5_1:+20889984	None:intergenic
AGGCAAGGGTGCTTCGGTTT+TGG	0.317188	5_1:-20890040	Msa0723340:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCATTTCTTCCATAAACTTG+AGG	+	chr5_1:20890464-20890483	None:intergenic	30.0%
TTATCAGTCTATCCTATAGT+AGG	-	chr5_1:20890539-20890558	Msa0723340:CDS	30.0%
CTCATAATTGAACACAACTT+GGG	+	chr5_1:20890594-20890613	None:intergenic	30.0%
TCTCATAATTGAACACAACT+TGG	+	chr5_1:20890595-20890614	None:intergenic	30.0%
AGTTGTGTTCAATTATGAGA+AGG	-	chr5_1:20890595-20890614	Msa0723340:CDS	30.0%
AACACTTTGAGCTAAGAATT+TGG	-	chr5_1:20890624-20890643	Msa0723340:CDS	30.0%
ACACTTTGAGCTAAGAATTT+GGG	-	chr5_1:20890625-20890644	Msa0723340:CDS	30.0%
ACGAAGAAAAAGATATTTGC+TGG	-	chr5_1:20890769-20890788	Msa0723340:CDS	30.0%
TCATTTCTTCCATAAACTTG+AGG	+	chr5_1:20890464-20890483	None:intergenic	30.0%
TTATCAGTCTATCCTATAGT+AGG	-	chr5_1:20890539-20890558	Msa0723340:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	20889969	20890952	20889969	ID=Msa0723340;Name=Msa0723340
chr5_1	mRNA	20889969	20890952	20889969	ID=Msa0723340-mRNA-1;Parent=Msa0723340;Name=Msa0723340-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|327
chr5_1	exon	20889969	20890952	20889969	ID=Msa0723340-mRNA-1:exon:8013;Parent=Msa0723340-mRNA-1
chr5_1	CDS	20889969	20890952	20889969	ID=Msa0723340-mRNA-1:cds;Parent=Msa0723340-mRNA-1

Gene Sequence

>Msa0723340

ATGGCTGAAGCTGTGCTTGAAGTTGTCCTTGACAATTTGAGCTCACTCGTTCAGAAGGAGATTGGCCTATTTCTTAGTTTTGAACAAGACTTTAAAAGTCTATCAAGCTTGCTCACTACAATGAAGGCTACTCTTGAAGATGCCGAGGAGAAACAGTTCACCGACAAAGCTATCAAGGATTGGCTGATTAAGCTTAAAGATGCTGCTTACATCCTCGACGACATCTTGGACGAGTGTGCGACTCAAGCTTTGGAGATGGAGTACAAAGGATTCAAGGGTGGACCTTCACATAAGGTACAAAGCTCTTTCTTATCTTCTTTTCATCCTAAGCATGTTGCTTTCCGTTACAAACTTGCTAAGAAAATGAAAAGGATAAGAGAGAGATTGGATGAAATAGCTGAAGAAAGGAATAAGTTTCATTTGACTGAGATAGTGAGAGAGAGAAGAAGCGGGGTTCTTGACTGGCGACAAACTACCTCAAATATCACTCAACCTCAAGTTTATGGAAGAAATGAAGATAAGAATCGAATTGTAGACTTTTTGGTTGATGATGCGTATGCTTGTGAGGATTTATCAGTCTATCCTATAGTAGGTCTTGGTGGACTTGGGAAAACAACACTTGCCCAAGTTGTGTTCAATTATGAGAAGGTAGTCGAACACTTTGAGCTAAGAATTTGGGTATGTGTTTCAGAAGATTTCAGTTTGAAGAGAAAGACAAAAGCCATCATAGAAGCTGCATCTGGCCATGCTTGTGAGGATTTAGATCTAGAGCCGCTGCAAAGAAAACTTTTGGATTTGCTACGAAGAAAAAGATATTTGCTGGTGTTGGATGACGTATGGGATGATGAACAAGAGAATTGGCAGAAATTGAGATCTGTGTTGGCTTGTGGAGGCAAGGGTGCTTCGGTTTTGGTAACCACTCGTCTTTCAAAGGTTGCAGCCAGGGCCGTCCCTGAGAATTCGGAGGCTCGGCTCTGGGAATGA

Protein sequence

>Msa0723340

MAEAVLEVVLDNLSSLVQKEIGLFLSFEQDFKSLSSLLTTMKATLEDAEEKQFTDKAIKDWLIKLKDAAYILDDILDECATQALEMEYKGFKGGPSHKVQSSFLSSFHPKHVAFRYKLAKKMKRIRERLDEIAEERNKFHLTEIVRERRSGVLDWRQTTSNITQPQVYGRNEDKNRIVDFLVDDAYACEDLSVYPIVGLGGLGKTTLAQVVFNYEKVVEHFELRIWVCVSEDFSLKRKTKAIIEAASGHACEDLDLEPLQRKLLDLLRRKRYLLVLDDVWDDEQENWQKLRSVLACGGKGASVLVTTRLSKVAARAVPENSEARLWE*