Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000550 | Msa0492880 | -0.801016 | 1.098350e-48 | -8.615850e-47 |
| Msa0007080 | Msa0492880 | 0.820424 | 6.910369e-53 | -8.615850e-47 |
| Msa0007230 | Msa0492880 | 0.820887 | 5.410061e-53 | -8.615850e-47 |
| Msa0027100 | Msa0492880 | 0.804665 | 1.936824e-49 | -8.615850e-47 |
| Msa0029850 | Msa0492880 | 0.800315 | 1.526576e-48 | -8.615850e-47 |
| Msa0031360 | Msa0492880 | 0.836534 | 8.847745e-57 | -8.615850e-47 |
| Msa0033440 | Msa0492880 | 0.802718 | 4.911891e-49 | -8.615850e-47 |
| Msa0036920 | Msa0492880 | 0.802814 | 4.691986e-49 | -8.615850e-47 |
| Msa0039330 | Msa0492880 | 0.808695 | 2.730036e-50 | -8.615850e-47 |
| Msa0048000 | Msa0492880 | 0.807956 | 3.922369e-50 | -8.615850e-47 |
Find 336 sgRNAs with CRISPR-Local
Find 663 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTAGGCTGTTAAGACA+GGG | 0.633330 | 3_4:+17077204 | None:intergenic |
| AAAACTGATTGGTTCAAGAG+AGG | 0.649856 | 3_4:-17080084 | Msa0492880:five_prime_UTR |
| AAAAGGAGGGAGGAGAGTGT+TGG | 0.533835 | 3_4:-17079764 | Msa0492880:five_prime_UTR |
| AAAATAGTGGACAAGTGCGG+AGG | 0.616007 | 3_4:-17082357 | Msa0492880:five_prime_UTR |
| AAACAACGCTTCCGGTTGGA+AGG | 0.549542 | 3_4:+17082183 | None:intergenic |
| AAACCGACTATGCTGATTAT+TGG | 0.261123 | 3_4:+17082855 | None:intergenic |
| AAACGTGGAGTCCTTCCCAG+AGG | 0.770799 | 3_4:-17080003 | Msa0492880:five_prime_UTR |
| AAAGAAGATGCCAAAGCATA+TGG | 0.384120 | 3_4:-17081467 | Msa0492880:five_prime_UTR |
| AAAGAGTTGGGATTGGGAGA+AGG | 0.585861 | 3_4:-17083035 | Msa0492880:five_prime_UTR |
| AAAGAGTTGTGATAGAATTT+TGG | 0.198221 | 3_4:-17080441 | Msa0492880:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCAACGATGAAGTTTCATA+TGG | + | chr3_4:17076265-17076284 | None:intergenic | 30.0% | |
| TTTGTTTGTTCTCACATTTG+AGG | - | chr3_4:17076508-17076527 | Msa0492880:three_prime_UTR | 30.0% | |
| TTATAGGGACTAAAAGTTGA+AGG | - | chr3_4:17077221-17077240 | Msa0492880:three_prime_UTR | 30.0% | |
| AAAACAATGGAGAGAGAAAA+AGG | + | chr3_4:17077741-17077760 | None:intergenic | 30.0% | |
| AATAAAAACAGATAGTTCGG+TGG | + | chr3_4:17077984-17078003 | None:intergenic | 30.0% | |
| CTTCTCAATAATCACTTGAA+AGG | + | chr3_4:17079426-17079445 | None:intergenic | 30.0% | |
| TCAAGTGATTATTGAGAAGT+TGG | - | chr3_4:17079427-17079446 | Msa0492880:intron | 30.0% | |
| TAGAAATCTCATCCAATAGT+TGG | + | chr3_4:17079631-17079650 | None:intergenic | 30.0% | |
| CTACAAATCCATTTGAATCT+AGG | - | chr3_4:17079729-17079748 | Msa0492880:five_prime_UTR | 30.0% | |
| ATATGGTAGAGATGATGATA+AGG | - | chr3_4:17079904-17079923 | Msa0492880:five_prime_UTR | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_4 | gene | 17076160 | 17086694 | 17076160 | ID=Msa0492880;Name=Msa0492880 |
| chr3_4 | mRNA | 17076160 | 17086694 | 17076160 | ID=Msa0492880-mRNA-1;Parent=Msa0492880;Name=Msa0492880-mRNA-1;_AED=0.10;_eAED=0.10;_QI=4041|0.83|0.71|1|0.16|0.14|7|787|82 |
| chr3_4 | exon | 17076160 | 17076812 | 17076160 | ID=Msa0492880-mRNA-1:exon:4811;Parent=Msa0492880-mRNA-1 |
| chr3_4 | exon | 17077118 | 17077327 | 17077118 | ID=Msa0492880-mRNA-1:exon:4810;Parent=Msa0492880-mRNA-1 |
| chr3_4 | exon | 17078008 | 17078114 | 17078008 | ID=Msa0492880-mRNA-1:exon:4809;Parent=Msa0492880-mRNA-1 |
| chr3_4 | exon | 17079548 | 17079574 | 17079548 | ID=Msa0492880-mRNA-1:exon:4808;Parent=Msa0492880-mRNA-1 |
| chr3_4 | exon | 17079665 | 17083480 | 17079665 | ID=Msa0492880-mRNA-1:exon:4807;Parent=Msa0492880-mRNA-1 |
| chr3_4 | exon | 17086325 | 17086383 | 17086325 | ID=Msa0492880-mRNA-1:exon:4806;Parent=Msa0492880-mRNA-1 |
| chr3_4 | exon | 17086490 | 17086694 | 17086490 | ID=Msa0492880-mRNA-1:exon:4805;Parent=Msa0492880-mRNA-1 |
| chr3_4 | five_prime_UTR | 17086490 | 17086694 | 17086490 | ID=Msa0492880-mRNA-1:five_prime_utr;Parent=Msa0492880-mRNA-1 |
| chr3_4 | five_prime_UTR | 17086325 | 17086383 | 17086325 | ID=Msa0492880-mRNA-1:five_prime_utr;Parent=Msa0492880-mRNA-1 |
| chr3_4 | five_prime_UTR | 17079704 | 17083480 | 17079704 | ID=Msa0492880-mRNA-1:five_prime_utr;Parent=Msa0492880-mRNA-1 |
| chr3_4 | CDS | 17079665 | 17079703 | 17079665 | ID=Msa0492880-mRNA-1:cds;Parent=Msa0492880-mRNA-1 |
| chr3_4 | CDS | 17079548 | 17079574 | 17079548 | ID=Msa0492880-mRNA-1:cds;Parent=Msa0492880-mRNA-1 |
| chr3_4 | CDS | 17078008 | 17078114 | 17078008 | ID=Msa0492880-mRNA-1:cds;Parent=Msa0492880-mRNA-1 |
| chr3_4 | CDS | 17077252 | 17077327 | 17077252 | ID=Msa0492880-mRNA-1:cds;Parent=Msa0492880-mRNA-1 |
| chr3_4 | three_prime_UTR | 17077118 | 17077251 | 17077118 | ID=Msa0492880-mRNA-1:three_prime_utr;Parent=Msa0492880-mRNA-1 |
| chr3_4 | three_prime_UTR | 17076160 | 17076812 | 17076160 | ID=Msa0492880-mRNA-1:three_prime_utr;Parent=Msa0492880-mRNA-1 |
| Gene Sequence |
>Msa0492880 ATGGGTCCCACTATTGGTCAAACCTTTTTGGTTGTGAAGGCTAAGAGGGACTCTGCGTTTGGAATGCTGGATCTGAAATTTGTTACTGATGATAGAATCAAGGATATCATTGTAGAGATGTGTGCATTTTGTGATATCAGGACTCAGGCATCCACTTCAACCACTTACTATTTATTGGTAGATGCTGATTCTGGTGGTGAGACCCTCCCAACCATTTCATCATCAACGGCAAGGAGAGATTGCAGCTGA |
| Protein sequence |
>Msa0492880 MGPTIGQTFLVVKAKRDSAFGMLDLKFVTDDRIKDIIVEMCAFCDIRTQASTSTTYYLLVDADSGGETLPTISSSTARRDCS* |
