AlfalfaGEDB | Msa0736210

M. sativa cultivar zhongmu-4 / Msa0736210

KEGG: K11266
GO: GO:0000003 GO:0000070 GO:0000278 GO:0000280 GO:0000785 GO:0000819 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007064 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016043 GO:0022402 GO:0022414 GO:0032116 GO:0032501 GO:0032502 GO:0032991 GO:0034086 GO:0034088 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0048285 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051276 GO:0061458 GO:0071840 GO:0097159 GO:0098813 GO:0140014 GO:1901363 GO:1903047
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736210	MCI17607.1	72.000	150	36	3	2	147	11	158	1.79e-61	197

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736210	sp\|Q9FGN7\|SCC4_ARATH	50.485	103	50	1	54	156	275	376	8.52e-23	97.1

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736210	A0A392Q0V7	72.000	150	36	3	2	147	11	158	8.55e-62	197

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0119960	Msa0736210	0.807740	4.361110e-50	-8.615850e-47
Msa0736210	Msa0766630	0.815961	7.068797e-52	-8.615850e-47
Msa0736210	Msa0777790	0.801527	8.634877e-49	-8.615850e-47
Msa0736210	Msa0802830	0.813692	2.250541e-51	-8.615850e-47
Msa0736210	Msa0885280	0.852737	3.792523e-61	-8.615850e-47
Msa0736210	Msa0885290	0.827066	1.921108e-54	-8.615850e-47
Msa0736210	Msa0907070	0.820098	8.205920e-53	-8.615850e-47
Msa0736210	Msa0935910	0.808446	3.085282e-50	-8.615850e-47
Msa0736210	Msa0954910	0.809075	2.263205e-50	-8.615850e-47
Msa0736210	Msa0959770	0.808752	2.654497e-50	-8.615850e-47
Msa0736210	Msa1014540	0.829755	4.310889e-55	-8.615850e-47
Msa0736210	Msa1136090	0.817485	3.217808e-52	-8.615850e-47
Msa0736210	Msa1160670	0.808999	2.350249e-50	-8.615850e-47
Msa0736210	Msa1249490	0.820311	7.335882e-53	-8.615850e-47
Msa0736210	Msa1283860	0.807544	4.799198e-50	-8.615850e-47
Msa0736210	Msa1297210	0.806149	9.463208e-50	-8.615850e-47
Msa0736210	Msa1397860	0.818788	1.632286e-52	-8.615850e-47
Msa0736210	Msa1411020	0.801797	7.601340e-49	-8.615850e-47
Msa0736210	Msa1424290	0.800324	1.520164e-48	-8.615850e-47
Msa0736210	Msa1434350	0.801140	1.036028e-48	-8.615850e-47
Msa0736210	Msa1438250	0.828933	6.823495e-55	-8.615850e-47
Msa0736210	Msa1439320	0.802485	5.485709e-49	-8.615850e-47
Msa0736210	Msa1452240	0.813154	2.954675e-51	-8.615850e-47
Msa0153910	Msa0736210	0.827004	1.987679e-54	-8.615850e-47
Msa0222890	Msa0736210	0.819943	8.906811e-53	-8.615850e-47
Msa0397210	Msa0736210	0.806994	6.276520e-50	-8.615850e-47
Msa0402290	Msa0736210	0.808215	3.455090e-50	-8.615850e-47
Msa0437990	Msa0736210	0.801598	8.347525e-49	-8.615850e-47
Msa0355220	Msa0736210	0.818150	2.277882e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736210	MtrunA17_Chr5g0433081	71.698	159	39	3	2	156	223	379	3.97e-65	211
Msa0736210	MtrunA17_Chr6g0469611	80.000	55	11	0	102	156	82	136	9.60e-24	92.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736210	AT5G51340.1	50.485	103	50	1	54	156	275	376	8.66e-24	97.1

Find 31 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATCTTGAATCAGTATATTT+TGG	0.103251	5_1:+52290977	Msa0736210:CDS
GATCTGATAGTTGTTGTATT+TGG	0.278509	5_1:+52291094	Msa0736210:CDS
ATCAAAGTCTTTCCAGTTCC+AGG	0.280895	5_1:+52290852	Msa0736210:CDS
ATCTGATAGTTGTTGTATTT+GGG	0.291382	5_1:+52291095	Msa0736210:CDS
ATGAATGGGTTCAGTTCAAT+TGG	0.315950	5_1:+52290947	Msa0736210:CDS
CTCCTTCACTATTGAAATGT+TGG	0.434137	5_1:-52290753	None:intergenic
ACAACAACTATCAGATCAAC+TGG	0.434611	5_1:-52291088	None:intergenic
GTGAAGATCAGACCTGGAAC+TGG	0.461240	5_1:-52290864	None:intergenic
CCAGGTCTGATCTTCACTAC+AGG	0.465404	5_1:+52290870	Msa0736210:CDS
TGTGGAAAGCGCTTACAGTC+GGG	0.494575	5_1:+52291142	Msa0736210:CDS
TCGGGGATGTGTATAATTCA+AGG	0.515356	5_1:+52291160	Msa0736210:CDS
CATCTCTTATCGATGGAGAA+TGG	0.520553	5_1:+52291041	Msa0736210:CDS
TGAATGGGTTCAGTTCAATT+GGG	0.525092	5_1:+52290948	Msa0736210:CDS
ACACAGCATAGGCACGAGCT+AGG	0.526453	5_1:+52290809	Msa0736210:CDS
GTGTGGAAAGCGCTTACAGT+CGG	0.533624	5_1:+52291141	Msa0736210:CDS
GGGAATGGCAGAACGACACT+AGG	0.544679	5_1:+52290998	Msa0736210:CDS
AACAGCTGAGAAACATGAAT+GGG	0.548330	5_1:+52290933	Msa0736210:CDS
GGAGCTAGTGAAGGAGCTGA+AGG	0.555780	5_1:+52290772	Msa0736210:CDS
GAACAGCTGAGAAACATGAA+TGG	0.557631	5_1:+52290932	Msa0736210:CDS
CACAGCATAGGCACGAGCTA+GGG	0.568797	5_1:+52290810	Msa0736210:CDS
TGGGTTCAGTTCAATTGGGA+GGG	0.569968	5_1:+52290952	Msa0736210:CDS
TGCCAACATTTCAATAGTGA+AGG	0.571763	5_1:+52290751	Msa0736210:CDS
CCTGTAGTGAAGATCAGACC+TGG	0.579186	5_1:-52290870	None:intergenic
TTAGCACCATCTCTTATCGA+TGG	0.587659	5_1:+52291034	Msa0736210:CDS
CAGGTCTGATCTTCACTACA+GGG	0.605048	5_1:+52290871	Msa0736210:CDS
CATTCTCCATCGATAAGAGA+TGG	0.605696	5_1:-52291040	None:intergenic
ATGGGTTCAGTTCAATTGGG+AGG	0.607181	5_1:+52290951	Msa0736210:CDS
GTGGAAAGCGCTTACAGTCG+GGG	0.615328	5_1:+52291143	Msa0736210:CDS
AATAGTGAAGGAGCTAGTGA+AGG	0.620883	5_1:+52290763	Msa0736210:CDS
GCATAGGCACGAGCTAGGGA+AGG	0.624203	5_1:+52290814	Msa0736210:CDS
AAAAGAAGCAAACACAGCAT+AGG	0.626769	5_1:+52290798	Msa0736210:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATCTTGAATCAGTATATTT+TGG	+	chr5_1:52290977-52290996	Msa0736210:CDS	20.0%
!!!	ATCTTGAATCAGTATATTTT+GGG	+	chr5_1:52290978-52290997	Msa0736210:CDS	20.0%
!	ATCTGATAGTTGTTGTATTT+GGG	+	chr5_1:52291095-52291114	Msa0736210:CDS	25.0%
!!!	ACTCCTTAAAAAGCTTTTTT+GGG	-	chr5_1:52291123-52291142	None:intergenic	25.0%
!!	GAATCAGTATATTTTGGGAA+TGG	+	chr5_1:52290983-52291002	Msa0736210:CDS	30.0%
	GATCTGATAGTTGTTGTATT+TGG	+	chr5_1:52291094-52291113	Msa0736210:CDS	30.0%
!!!	CACTCCTTAAAAAGCTTTTT+TGG	-	chr5_1:52291124-52291143	None:intergenic	30.0%
!!	AAAAAGCTTTTTAAGGAGTG+TGG	+	chr5_1:52291124-52291143	Msa0736210:CDS	30.0%
	TGCCAACATTTCAATAGTGA+AGG	+	chr5_1:52290751-52290770	Msa0736210:CDS	35.0%
	CTCCTTCACTATTGAAATGT+TGG	-	chr5_1:52290756-52290775	None:intergenic	35.0%
	AAAAGAAGCAAACACAGCAT+AGG	+	chr5_1:52290798-52290817	Msa0736210:CDS	35.0%
	AACAGCTGAGAAACATGAAT+GGG	+	chr5_1:52290933-52290952	Msa0736210:CDS	35.0%
!	ATGAATGGGTTCAGTTCAAT+TGG	+	chr5_1:52290947-52290966	Msa0736210:CDS	35.0%
!	TGAATGGGTTCAGTTCAATT+GGG	+	chr5_1:52290948-52290967	Msa0736210:CDS	35.0%
	ACAACAACTATCAGATCAAC+TGG	-	chr5_1:52291091-52291110	None:intergenic	35.0%
!	GTGCCCAAAAAAGCTTTTTA+AGG	+	chr5_1:52291117-52291136	Msa0736210:CDS	35.0%
	AATAGTGAAGGAGCTAGTGA+AGG	+	chr5_1:52290763-52290782	Msa0736210:CDS	40.0%
	ATCAAAGTCTTTCCAGTTCC+AGG	+	chr5_1:52290852-52290871	Msa0736210:CDS	40.0%
	GAACAGCTGAGAAACATGAA+TGG	+	chr5_1:52290932-52290951	Msa0736210:CDS	40.0%
	TTAGCACCATCTCTTATCGA+TGG	+	chr5_1:52291034-52291053	Msa0736210:CDS	40.0%
	CATTCTCCATCGATAAGAGA+TGG	-	chr5_1:52291043-52291062	None:intergenic	40.0%
	CATCTCTTATCGATGGAGAA+TGG	+	chr5_1:52291041-52291060	Msa0736210:CDS	40.0%
!!	GGTGCATAGACATCACTTTT+AGG	-	chr5_1:52291070-52291089	None:intergenic	40.0%
	TCGGGGATGTGTATAATTCA+AGG	+	chr5_1:52291160-52291179	Msa0736210:CDS	40.0%
	CAGGTCTGATCTTCACTACA+GGG	+	chr5_1:52290871-52290890	Msa0736210:CDS	45.0%
	ATGGGTTCAGTTCAATTGGG+AGG	+	chr5_1:52290951-52290970	Msa0736210:CDS	45.0%
	TGGGTTCAGTTCAATTGGGA+GGG	+	chr5_1:52290952-52290971	Msa0736210:CDS	45.0%
	GTGAAGATCAGACCTGGAAC+TGG	-	chr5_1:52290867-52290886	None:intergenic	50.0%
	CCTGTAGTGAAGATCAGACC+TGG	-	chr5_1:52290873-52290892	None:intergenic	50.0%
	CCAGGTCTGATCTTCACTAC+AGG	+	chr5_1:52290870-52290889	Msa0736210:CDS	50.0%
!!	GTGTGGAAAGCGCTTACAGT+CGG	+	chr5_1:52291141-52291160	Msa0736210:CDS	50.0%
!!	TGTGGAAAGCGCTTACAGTC+GGG	+	chr5_1:52291142-52291161	Msa0736210:CDS	50.0%
	GGAGCTAGTGAAGGAGCTGA+AGG	+	chr5_1:52290772-52290791	Msa0736210:CDS	55.0%
	ACACAGCATAGGCACGAGCT+AGG	+	chr5_1:52290809-52290828	Msa0736210:CDS	55.0%
	CACAGCATAGGCACGAGCTA+GGG	+	chr5_1:52290810-52290829	Msa0736210:CDS	55.0%
	GGGAATGGCAGAACGACACT+AGG	+	chr5_1:52290998-52291017	Msa0736210:CDS	55.0%
!!	GTGGAAAGCGCTTACAGTCG+GGG	+	chr5_1:52291143-52291162	Msa0736210:CDS	55.0%
	GCATAGGCACGAGCTAGGGA+AGG	+	chr5_1:52290814-52290833	Msa0736210:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	52290713	52291195	52290713	ID=Msa0736210;Name=Msa0736210
chr5_1	mRNA	52290713	52291195	52290713	ID=Msa0736210-mRNA-1;Parent=Msa0736210;Name=Msa0736210-mRNA-1;_AED=0.84;_eAED=0.84;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|160
chr5_1	exon	52290713	52291195	52290713	ID=Msa0736210-mRNA-1:exon:14763;Parent=Msa0736210-mRNA-1
chr5_1	CDS	52290713	52291195	52290713	ID=Msa0736210-mRNA-1:cds;Parent=Msa0736210-mRNA-1

Gene Sequence

>Msa0736210

ATGACTGAATTATGTTTTATTACATATAATACGTTCTATGCCAACATTTCAATAGTGAAGGAGCTAGTGAAGGAGCTGAAGGCTGAAAAGAAGCAAACACAGCATAGGCACGAGCTAGGGAAGGAGCTCAGTGCTTTAGATCAAAGTCTTTCCAGTTCCAGGTCTGATCTTCACTACAGGGAAAGAGACGCACTGTCAGAAAAGAAAGCAACGATTCAAGAACAGCTGAGAAACATGAATGGGTTCAGTTCAATTGGGAGGGAATATCTTGAATCAGTATATTTTGGGAATGGCAGAACGACACTAGGAGATAAGCTTCAGTTAGCACCATCTCTTATCGATGGAGAATGGCTTCCTAAAAGTGATGTCTATGCACCAGTTGATCTGATAGTTGTTGTATTTGGGTGCCCAAAAAAGCTTTTTAAGGAGTGTGGAAAGCGCTTACAGTCGGGGATGTGTATAATTCAAGGTTGTTCAACATAA

Protein sequence

>Msa0736210

MTELCFITYNTFYANISIVKELVKELKAEKKQTQHRHELGKELSALDQSLSSSRSDLHYRERDALSEKKATIQEQLRNMNGFSSIGREYLESVYFGNGRTTLGDKLQLAPSLIDGEWLPKSDVYAPVDLIVVVFGCPKKLFKECGKRLQSGMCIIQGCST*