AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0546710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0546710 MtrunA17_Chr4g0028501 37.629 194 110 6 19 207 20 207 7.35e-40 135
Msa0546710 MtrunA17_Chr3g0089081 35.897 195 114 8 20 203 70 264 1.43e-33 120
Msa0546710 MtrunA17_Chr3g0089091 35.385 195 115 8 20 203 79 273 1.43e-33 121
Msa0546710 MtrunA17_Chr2g0287471 37.572 173 101 4 26 192 163 334 3.92e-33 121
Msa0546710 MtrunA17_Chr6g0479301 36.585 205 110 10 18 202 76 280 1.73e-30 113
Msa0546710 MtrunA17_Chr5g0417341 34.158 202 116 10 19 203 27 228 3.00e-29 108
Msa0546710 MtrunA17_Chr5g0439761 35.385 195 113 9 21 203 21 214 3.55e-29 108
Msa0546710 MtrunA17_Chr2g0287571 33.152 184 114 5 15 193 8 187 6.23e-28 104
Msa0546710 MtrunA17_Chr5g0417321 33.500 200 118 10 19 203 27 226 8.33e-28 105
Msa0546710 MtrunA17_Chr6g0479341 33.333 207 110 10 18 202 69 269 1.62e-26 102
Msa0546710 MtrunA17_Chr5g0417371 31.839 223 133 12 1 205 6 227 8.04e-26 99.8
Msa0546710 MtrunA17_Chr8g0350611 34.123 211 114 10 12 203 95 299 1.23e-25 100
Msa0546710 MtrunA17_Chr2g0287541 31.088 193 122 7 16 202 3 190 1.61e-25 98.2
Msa0546710 MtrunA17_Chr5g0417391 32.093 215 130 11 3 202 13 226 9.17e-23 92.0
Msa0546710 MtrunA17_Chr2g0287561 38.095 126 70 5 75 196 16 137 3.12e-21 85.9
Msa0546710 MtrunA17_Chr1g0170111 31.282 195 118 8 18 196 102 296 5.14e-21 88.6
Msa0546710 MtrunA17_Chr6g0483091 32.692 208 122 10 2 192 7 213 1.64e-20 85.9
Msa0546710 MtrunA17_Chr8g0339941 33.168 202 110 11 19 203 415 608 4.30e-20 87.8
Msa0546710 MtrunA17_Chr8g0347611 31.694 183 101 6 19 180 42 221 8.84e-19 82.0
Msa0546710 MtrunA17_Chr5g0417381 29.032 217 138 11 3 204 12 227 5.34e-18 79.0
Msa0546710 MtrunA17_Chr5g0417401 28.571 217 139 11 3 204 12 227 1.27e-15 72.8
Msa0546710 MtrunA17_Chr2g0333421 31.405 121 73 6 92 205 11 128 4.77e-15 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0546710 AT2G02990.1 31.193 218 135 10 1 203 11 228 8.17e-24 94.7
Msa0546710 AT1G26820.1 29.545 220 137 11 4 205 3 222 2.43e-22 90.9
Msa0546710 AT1G14220.1 31.313 198 122 9 7 190 15 212 2.50e-20 85.5
Msa0546710 AT2G39780.1 28.796 191 112 6 11 180 23 210 5.18e-19 82.8
Msa0546710 AT1G14210.1 27.477 222 144 10 1 205 7 228 4.55e-18 80.1

Find 54 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGATCTTTGATGTCATTT+AGG 0.156708 4_1:-38928981 None:intergenic
AAGTAAATAACTCTAAATTT+TGG 0.171378 4_1:+38928878 Msa0546710:CDS
GTCTCATTTGTGCAATGATA+TGG 0.225299 4_1:-38928692 None:intergenic
AAGATCTTTGATGTCATTTA+GGG 0.233880 4_1:-38928980 None:intergenic
ATTGAAGTCTAGGCTTAATA+AGG 0.255354 4_1:+38928834 Msa0546710:CDS
CCACAATTTGTACCAGTATT+AGG 0.259803 4_1:-38929171 None:intergenic
GTTATTGTTCTCTTTGTTCC+TGG 0.282679 4_1:+38928514 Msa0546710:CDS
CATTTAGGGCATACAAATCT+AGG 0.293125 4_1:-38928966 None:intergenic
TAACTTTATTTAATAACTTC+TGG 0.331114 4_1:-38929210 None:intergenic
GTAGTAGTCAAAGTTTATTT+TGG 0.342077 4_1:-38928555 None:intergenic
GTTAATAAAATTATATATGA+TGG 0.387686 4_1:+38929229 Msa0546710:three_prime_UTR
TTATTATCACAATGAAGCTT+AGG 0.393545 4_1:-38929084 None:intergenic
AGTCTAGGCTTAATAAGGTT+TGG 0.419222 4_1:+38928839 Msa0546710:CDS
TATATAAGTTGTCCTAATAC+TGG 0.424971 4_1:+38929159 Msa0546710:CDS
TTTGTGCAATGATATGGGTT+AGG 0.440239 4_1:-38928686 None:intergenic
TATTATCACAATGAAGCTTA+GGG 0.458506 4_1:-38929083 None:intergenic
AAGAGAACAATAACGACATA+AGG 0.465778 4_1:-38928505 None:intergenic
AACTTTAACACCTGGTGGAT+CGG 0.470033 4_1:+38929011 Msa0546710:CDS
ATTACTATTGTGTGGGTGCA+TGG 0.475775 4_1:+38929258 Msa0546710:three_prime_UTR
CTTAATAAAACTTTAACACC+TGG 0.479447 4_1:+38929003 Msa0546710:CDS
CCTAATACTGGTACAAATTG+TGG 0.488261 4_1:+38929171 Msa0546710:CDS
AGCTTGATCAATTGAAGTCT+AGG 0.490799 4_1:+38928824 Msa0546710:intron
GTTGGTTATTACTATTGTGT+GGG 0.494348 4_1:+38929251 Msa0546710:three_prime_UTR
CTAAATAAATATGGAGGAGA+CGG 0.501939 4_1:+38929322 Msa0546710:three_prime_UTR
TCTCATTTGTGCAATGATAT+GGG 0.503124 4_1:-38928691 None:intergenic
ATAAAATTATATATGATGGT+TGG 0.504489 4_1:+38929233 Msa0546710:three_prime_UTR
GGTTGGTTATTACTATTGTG+TGG 0.512296 4_1:+38929250 Msa0546710:three_prime_UTR
ATTTCAGCATCCACGGATTG+TGG 0.518420 4_1:+38928645 Msa0546710:CDS
TCTGCTTCAATACCACCAAC+AGG 0.519484 4_1:+38929136 Msa0546710:CDS
TGTTCTGCGGCCACAATCCG+TGG 0.527412 4_1:-38928655 None:intergenic
CCTCAAAATTTCAGCATCCA+CGG 0.533572 4_1:+38928638 Msa0546710:CDS
AATAAAACTTTAACACCTGG+TGG 0.549310 4_1:+38929006 Msa0546710:CDS
ATAAATATGGAGGAGACGGA+GGG 0.564155 4_1:+38929326 Msa0546710:three_prime_UTR
ATGAAGCTTAGGGTTAGATC+CGG 0.566541 4_1:-38929073 None:intergenic
AATAAATATGGAGGAGACGG+AGG 0.573129 4_1:+38929325 Msa0546710:three_prime_UTR
CAGTAGATGATTGCAAGTCC+AGG 0.575829 4_1:-38928532 None:intergenic
ATTTAGGGCATACAAATCTA+GGG 0.575925 4_1:-38928965 None:intergenic
GCTTTCGTCGCCGATCCACC+AGG 0.584621 4_1:-38929021 None:intergenic
ATCGAGAGTGCTATAAACTT+AGG 0.588616 4_1:+38929048 Msa0546710:CDS
GATTGTGGCCGCAGAACAAG+AGG 0.588738 4_1:+38928660 Msa0546710:CDS
AGGACAACTTATATACCTGT+TGG 0.591332 4_1:-38929151 None:intergenic
ACAACTTATATACCTGTTGG+TGG 0.612648 4_1:-38929148 None:intergenic
AGTGCTATAAACTTAGGCAC+CGG 0.617633 4_1:+38929054 Msa0546710:CDS
TTAGGCTTCCTCTTGTTCTG+CGG 0.625435 4_1:-38928668 None:intergenic
TCATTGCACAAATGAGACAC+TGG 0.631016 4_1:+38928697 Msa0546710:CDS
TGAGACACTGGAGCCGCATG+TGG 0.638823 4_1:+38928709 Msa0546710:CDS
TCGAGATAGCATACCACATG+CGG 0.641657 4_1:-38928722 None:intergenic
ACTACATGCTAGCAGAAACA+TGG 0.642833 4_1:+38928573 Msa0546710:CDS
GCAAGCTTGAAGTAATCGAA+TGG 0.652108 4_1:-38928943 None:intergenic
AGCTTGAAGTAATCGAATGG+TGG 0.656716 4_1:-38928940 None:intergenic
TAAATATGGAGGAGACGGAG+GGG 0.657667 4_1:+38929327 Msa0546710:three_prime_UTR
AATGATGAGTGGAAGAAACA+TGG 0.696649 4_1:+38928901 Msa0546710:CDS
GTAATCGAATGGTGGCATCG+TGG 0.710181 4_1:-38928932 None:intergenic
TAAGTTTATAGCACTCTCGA+TGG 0.713022 4_1:-38929046 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAATAAAAAATAACAAA+AGG - chr4_1:38928472-38928491 None:intergenic 10.0%
!!! TTTGTTATTTTTTATTTCTT+TGG + chr4_1:38928472-38928491 Msa0546710:five_prime_UTR 10.0%
!!! ATTTTACTATTTTTATCTTA+TGG + chr4_1:38928758-38928777 Msa0546710:intron 10.0%
!!! TTTACTTTTAATTTTAAAGA+CGG - chr4_1:38928865-38928884 None:intergenic 10.0%
!! GTTAATAAAATTATATATGA+TGG + chr4_1:38929229-38929248 Msa0546710:three_prime_UTR 10.0%
!!! ATATGTTTTCTAAATAAATA+TGG + chr4_1:38929313-38929332 Msa0546710:three_prime_UTR 10.0%
!! AAGAAATAAAAAATAACAAA+AGG - chr4_1:38928472-38928491 None:intergenic 10.0%
!!! TTTGTTATTTTTTATTTCTT+TGG + chr4_1:38928472-38928491 Msa0546710:five_prime_UTR 10.0%
!!! ATTTTACTATTTTTATCTTA+TGG + chr4_1:38928758-38928777 Msa0546710:intron 10.0%
!!! TTTACTTTTAATTTTAAAGA+CGG - chr4_1:38928865-38928884 None:intergenic 10.0%
!! GTTAATAAAATTATATATGA+TGG + chr4_1:38929229-38929248 Msa0546710:three_prime_UTR 10.0%
!!! ATATGTTTTCTAAATAAATA+TGG + chr4_1:38929313-38929332 Msa0546710:three_prime_UTR 10.0%
!! ATAGTAAAATCAAAGATAAT+TGG - chr4_1:38928748-38928767 None:intergenic 15.0%
!!! AAGTAAATAACTCTAAATTT+TGG + chr4_1:38928878-38928897 Msa0546710:CDS 15.0%
!!! TAACTTTATTTAATAACTTC+TGG - chr4_1:38929213-38929232 None:intergenic 15.0%
!! ATAAAATTATATATGATGGT+TGG + chr4_1:38929233-38929252 Msa0546710:three_prime_UTR 15.0%
!! AAAAAATAACAAAAGGAAAT+TGG - chr4_1:38928465-38928484 None:intergenic 15.0%
!! ATAGTAAAATCAAAGATAAT+TGG - chr4_1:38928748-38928767 None:intergenic 15.0%
!!! AAGTAAATAACTCTAAATTT+TGG + chr4_1:38928878-38928897 Msa0546710:CDS 15.0%
!!! TAACTTTATTTAATAACTTC+TGG - chr4_1:38929213-38929232 None:intergenic 15.0%
!! ATAAAATTATATATGATGGT+TGG + chr4_1:38929233-38929252 Msa0546710:three_prime_UTR 15.0%
!!! TGAAATTTTGAGGTATATTA+GGG - chr4_1:38928631-38928650 None:intergenic 20.0%
!! TAAAAACTCTAGTAATTTGA+TGG + chr4_1:38929104-38929123 Msa0546710:CDS 20.0%
!!! TGTTTTCTAAATAAATATGG+AGG + chr4_1:38929316-38929335 Msa0546710:three_prime_UTR 20.0%
!!! TGAAATTTTGAGGTATATTA+GGG - chr4_1:38928631-38928650 None:intergenic 20.0%
!! TAAAAACTCTAGTAATTTGA+TGG + chr4_1:38929104-38929123 Msa0546710:CDS 20.0%
!!! TGTTTTCTAAATAAATATGG+AGG + chr4_1:38929316-38929335 Msa0546710:three_prime_UTR 20.0%
!! GTAGTAGTCAAAGTTTATTT+TGG - chr4_1:38928558-38928577 None:intergenic 25.0%
!! GATTTGTTTAGACAAAAAGT+TGG - chr4_1:38928602-38928621 None:intergenic 25.0%
!!! CTGAAATTTTGAGGTATATT+AGG - chr4_1:38928632-38928651 None:intergenic 25.0%
!!! CTATTTTTATCTTATGGTGT+TGG + chr4_1:38928764-38928783 Msa0546710:intron 25.0%
!! AAGATCTTTGATGTCATTTA+GGG - chr4_1:38928983-38929002 None:intergenic 25.0%
! CTTAATAAAACTTTAACACC+TGG + chr4_1:38929003-38929022 Msa0546710:CDS 25.0%
! TATTATCACAATGAAGCTTA+GGG - chr4_1:38929086-38929105 None:intergenic 25.0%
! TTATTATCACAATGAAGCTT+AGG - chr4_1:38929087-38929106 None:intergenic 25.0%
! TATATAAGTTGTCCTAATAC+TGG + chr4_1:38929159-38929178 Msa0546710:CDS 25.0%
!! GTAGTAGTCAAAGTTTATTT+TGG - chr4_1:38928558-38928577 None:intergenic 25.0%
!! GATTTGTTTAGACAAAAAGT+TGG - chr4_1:38928602-38928621 None:intergenic 25.0%
!!! CTGAAATTTTGAGGTATATT+AGG - chr4_1:38928632-38928651 None:intergenic 25.0%
!!! CTATTTTTATCTTATGGTGT+TGG + chr4_1:38928764-38928783 Msa0546710:intron 25.0%
!! AAGATCTTTGATGTCATTTA+GGG - chr4_1:38928983-38929002 None:intergenic 25.0%
! CTTAATAAAACTTTAACACC+TGG + chr4_1:38929003-38929022 Msa0546710:CDS 25.0%
! TATTATCACAATGAAGCTTA+GGG - chr4_1:38929086-38929105 None:intergenic 25.0%
! TTATTATCACAATGAAGCTT+AGG - chr4_1:38929087-38929106 None:intergenic 25.0%
! TATATAAGTTGTCCTAATAC+TGG + chr4_1:38929159-38929178 Msa0546710:CDS 25.0%
AAGAGAACAATAACGACATA+AGG - chr4_1:38928508-38928527 None:intergenic 30.0%
TTGTTTAGACAAAAAGTTGG+TGG - chr4_1:38928599-38928618 None:intergenic 30.0%
TCTCATTTGTGCAATGATAT+GGG - chr4_1:38928694-38928713 None:intergenic 30.0%
ATTGAAGTCTAGGCTTAATA+AGG + chr4_1:38928834-38928853 Msa0546710:CDS 30.0%
!!! CTAAATTTTGGAATGATGAG+TGG + chr4_1:38928890-38928909 Msa0546710:CDS 30.0%
! ATTTAGGGCATACAAATCTA+GGG - chr4_1:38928968-38928987 None:intergenic 30.0%
! GAAGATCTTTGATGTCATTT+AGG - chr4_1:38928984-38929003 None:intergenic 30.0%
AATAAAACTTTAACACCTGG+TGG + chr4_1:38929006-38929025 Msa0546710:CDS 30.0%
GTTGGTTATTACTATTGTGT+GGG + chr4_1:38929251-38929270 Msa0546710:three_prime_UTR 30.0%
CTAAATAAATATGGAGGAGA+CGG + chr4_1:38929322-38929341 Msa0546710:three_prime_UTR 30.0%
AAGAGAACAATAACGACATA+AGG - chr4_1:38928508-38928527 None:intergenic 30.0%
TTGTTTAGACAAAAAGTTGG+TGG - chr4_1:38928599-38928618 None:intergenic 30.0%
TCTCATTTGTGCAATGATAT+GGG - chr4_1:38928694-38928713 None:intergenic 30.0%
ATTGAAGTCTAGGCTTAATA+AGG + chr4_1:38928834-38928853 Msa0546710:CDS 30.0%
!!! CTAAATTTTGGAATGATGAG+TGG + chr4_1:38928890-38928909 Msa0546710:CDS 30.0%
! ATTTAGGGCATACAAATCTA+GGG - chr4_1:38928968-38928987 None:intergenic 30.0%
! GAAGATCTTTGATGTCATTT+AGG - chr4_1:38928984-38929003 None:intergenic 30.0%
AATAAAACTTTAACACCTGG+TGG + chr4_1:38929006-38929025 Msa0546710:CDS 30.0%
GTTGGTTATTACTATTGTGT+GGG + chr4_1:38929251-38929270 Msa0546710:three_prime_UTR 30.0%
CTAAATAAATATGGAGGAGA+CGG + chr4_1:38929322-38929341 Msa0546710:three_prime_UTR 30.0%
GTTATTGTTCTCTTTGTTCC+TGG + chr4_1:38928514-38928533 Msa0546710:CDS 35.0%
TTTGTGCAATGATATGGGTT+AGG - chr4_1:38928689-38928708 None:intergenic 35.0%
GTCTCATTTGTGCAATGATA+TGG - chr4_1:38928695-38928714 None:intergenic 35.0%
TAGACTTCAATTGATCAAGC+TGG - chr4_1:38928825-38928844 None:intergenic 35.0%
AGCTTGATCAATTGAAGTCT+AGG + chr4_1:38928824-38928843 Msa0546710:intron 35.0%
AGTCTAGGCTTAATAAGGTT+TGG + chr4_1:38928839-38928858 Msa0546710:CDS 35.0%
AATGATGAGTGGAAGAAACA+TGG + chr4_1:38928901-38928920 Msa0546710:CDS 35.0%
! CATTTAGGGCATACAAATCT+AGG - chr4_1:38928969-38928988 None:intergenic 35.0%
TAAGTTTATAGCACTCTCGA+TGG - chr4_1:38929049-38929068 None:intergenic 35.0%
!! ATCGAGAGTGCTATAAACTT+AGG + chr4_1:38929048-38929067 Msa0546710:CDS 35.0%
ACAACTTATATACCTGTTGG+TGG - chr4_1:38929151-38929170 None:intergenic 35.0%
AGGACAACTTATATACCTGT+TGG - chr4_1:38929154-38929173 None:intergenic 35.0%
CCACAATTTGTACCAGTATT+AGG - chr4_1:38929174-38929193 None:intergenic 35.0%
CCTAATACTGGTACAAATTG+TGG + chr4_1:38929171-38929190 Msa0546710:CDS 35.0%
! GGTTGGTTATTACTATTGTG+TGG + chr4_1:38929250-38929269 Msa0546710:three_prime_UTR 35.0%
GTTATTGTTCTCTTTGTTCC+TGG + chr4_1:38928514-38928533 Msa0546710:CDS 35.0%
TTTGTGCAATGATATGGGTT+AGG - chr4_1:38928689-38928708 None:intergenic 35.0%
GTCTCATTTGTGCAATGATA+TGG - chr4_1:38928695-38928714 None:intergenic 35.0%
TAGACTTCAATTGATCAAGC+TGG - chr4_1:38928825-38928844 None:intergenic 35.0%
AGCTTGATCAATTGAAGTCT+AGG + chr4_1:38928824-38928843 Msa0546710:intron 35.0%
AGTCTAGGCTTAATAAGGTT+TGG + chr4_1:38928839-38928858 Msa0546710:CDS 35.0%
AATGATGAGTGGAAGAAACA+TGG + chr4_1:38928901-38928920 Msa0546710:CDS 35.0%
! CATTTAGGGCATACAAATCT+AGG - chr4_1:38928969-38928988 None:intergenic 35.0%
TAAGTTTATAGCACTCTCGA+TGG - chr4_1:38929049-38929068 None:intergenic 35.0%
!! ATCGAGAGTGCTATAAACTT+AGG + chr4_1:38929048-38929067 Msa0546710:CDS 35.0%
ACAACTTATATACCTGTTGG+TGG - chr4_1:38929151-38929170 None:intergenic 35.0%
AGGACAACTTATATACCTGT+TGG - chr4_1:38929154-38929173 None:intergenic 35.0%
CCACAATTTGTACCAGTATT+AGG - chr4_1:38929174-38929193 None:intergenic 35.0%
CCTAATACTGGTACAAATTG+TGG + chr4_1:38929171-38929190 Msa0546710:CDS 35.0%
! GGTTGGTTATTACTATTGTG+TGG + chr4_1:38929250-38929269 Msa0546710:three_prime_UTR 35.0%
ACTACATGCTAGCAGAAACA+TGG + chr4_1:38928573-38928592 Msa0546710:CDS 40.0%
CCTCAAAATTTCAGCATCCA+CGG + chr4_1:38928638-38928657 Msa0546710:CDS 40.0%
TCATTGCACAAATGAGACAC+TGG + chr4_1:38928697-38928716 Msa0546710:CDS 40.0%
AGCTTGAAGTAATCGAATGG+TGG - chr4_1:38928943-38928962 None:intergenic 40.0%
GCAAGCTTGAAGTAATCGAA+TGG - chr4_1:38928946-38928965 None:intergenic 40.0%
! AACTTTAACACCTGGTGGAT+CGG + chr4_1:38929011-38929030 Msa0546710:CDS 40.0%
AGTGCTATAAACTTAGGCAC+CGG + chr4_1:38929054-38929073 Msa0546710:CDS 40.0%
ATGAAGCTTAGGGTTAGATC+CGG - chr4_1:38929076-38929095 None:intergenic 40.0%
ATTACTATTGTGTGGGTGCA+TGG + chr4_1:38929258-38929277 Msa0546710:three_prime_UTR 40.0%
AATAAATATGGAGGAGACGG+AGG + chr4_1:38929325-38929344 Msa0546710:three_prime_UTR 40.0%
ATAAATATGGAGGAGACGGA+GGG + chr4_1:38929326-38929345 Msa0546710:three_prime_UTR 40.0%
ACTACATGCTAGCAGAAACA+TGG + chr4_1:38928573-38928592 Msa0546710:CDS 40.0%
CCTCAAAATTTCAGCATCCA+CGG + chr4_1:38928638-38928657 Msa0546710:CDS 40.0%
TCATTGCACAAATGAGACAC+TGG + chr4_1:38928697-38928716 Msa0546710:CDS 40.0%
AGCTTGAAGTAATCGAATGG+TGG - chr4_1:38928943-38928962 None:intergenic 40.0%
GCAAGCTTGAAGTAATCGAA+TGG - chr4_1:38928946-38928965 None:intergenic 40.0%
! AACTTTAACACCTGGTGGAT+CGG + chr4_1:38929011-38929030 Msa0546710:CDS 40.0%
AGTGCTATAAACTTAGGCAC+CGG + chr4_1:38929054-38929073 Msa0546710:CDS 40.0%
ATGAAGCTTAGGGTTAGATC+CGG - chr4_1:38929076-38929095 None:intergenic 40.0%
ATTACTATTGTGTGGGTGCA+TGG + chr4_1:38929258-38929277 Msa0546710:three_prime_UTR 40.0%
AATAAATATGGAGGAGACGG+AGG + chr4_1:38929325-38929344 Msa0546710:three_prime_UTR 40.0%
ATAAATATGGAGGAGACGGA+GGG + chr4_1:38929326-38929345 Msa0546710:three_prime_UTR 40.0%
CAGTAGATGATTGCAAGTCC+AGG - chr4_1:38928535-38928554 None:intergenic 45.0%
! CCGTGGATGCTGAAATTTTG+AGG - chr4_1:38928641-38928660 None:intergenic 45.0%
! ATTTCAGCATCCACGGATTG+TGG + chr4_1:38928645-38928664 Msa0546710:CDS 45.0%
TTAGGCTTCCTCTTGTTCTG+CGG - chr4_1:38928671-38928690 None:intergenic 45.0%
TCGAGATAGCATACCACATG+CGG - chr4_1:38928725-38928744 None:intergenic 45.0%
TCTGCTTCAATACCACCAAC+AGG + chr4_1:38929136-38929155 Msa0546710:CDS 45.0%
TAAATATGGAGGAGACGGAG+GGG + chr4_1:38929327-38929346 Msa0546710:three_prime_UTR 45.0%
CAGTAGATGATTGCAAGTCC+AGG - chr4_1:38928535-38928554 None:intergenic 45.0%
! CCGTGGATGCTGAAATTTTG+AGG - chr4_1:38928641-38928660 None:intergenic 45.0%
! ATTTCAGCATCCACGGATTG+TGG + chr4_1:38928645-38928664 Msa0546710:CDS 45.0%
TTAGGCTTCCTCTTGTTCTG+CGG - chr4_1:38928671-38928690 None:intergenic 45.0%
TCGAGATAGCATACCACATG+CGG - chr4_1:38928725-38928744 None:intergenic 45.0%
TCTGCTTCAATACCACCAAC+AGG + chr4_1:38929136-38929155 Msa0546710:CDS 45.0%
TAAATATGGAGGAGACGGAG+GGG + chr4_1:38929327-38929346 Msa0546710:three_prime_UTR 45.0%
!! ATAAAATAAAATAAAGTAAA+TGG - chr4_1:38928798-38928817 None:intergenic 5.0%
!! ATAAAATAAAATAAAGTAAA+TGG - chr4_1:38928798-38928817 None:intergenic 5.0%
GTAATCGAATGGTGGCATCG+TGG - chr4_1:38928935-38928954 None:intergenic 50.0%
GTAATCGAATGGTGGCATCG+TGG - chr4_1:38928935-38928954 None:intergenic 50.0%
GATTGTGGCCGCAGAACAAG+AGG + chr4_1:38928660-38928679 Msa0546710:CDS 55.0%
GATTGTGGCCGCAGAACAAG+AGG + chr4_1:38928660-38928679 Msa0546710:CDS 55.0%
TGTTCTGCGGCCACAATCCG+TGG - chr4_1:38928658-38928677 None:intergenic 60.0%
TGAGACACTGGAGCCGCATG+TGG + chr4_1:38928709-38928728 Msa0546710:CDS 60.0%
TGTTCTGCGGCCACAATCCG+TGG - chr4_1:38928658-38928677 None:intergenic 60.0%
TGAGACACTGGAGCCGCATG+TGG + chr4_1:38928709-38928728 Msa0546710:CDS 60.0%
GCTTTCGTCGCCGATCCACC+AGG - chr4_1:38929024-38929043 None:intergenic 65.0%
GCTTTCGTCGCCGATCCACC+AGG - chr4_1:38929024-38929043 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 38928464 38929370 38928464 ID=Msa0546710;Name=Msa0546710
chr4_1 mRNA 38928464 38929370 38928464 ID=Msa0546710-mRNA-1;Parent=Msa0546710;Name=Msa0546710-mRNA-1;_AED=0.00;_eAED=0.00;_QI=45|1|1|1|1|1|2|143|207
chr4_1 exon 38928464 38928730 38928464 ID=Msa0546710-mRNA-1:exon:9924;Parent=Msa0546710-mRNA-1
chr4_1 exon 38928826 38929370 38928826 ID=Msa0546710-mRNA-1:exon:9925;Parent=Msa0546710-mRNA-1
chr4_1 five_prime_UTR 38928464 38928508 38928464 ID=Msa0546710-mRNA-1:five_prime_utr;Parent=Msa0546710-mRNA-1
chr4_1 CDS 38928509 38928730 38928509 ID=Msa0546710-mRNA-1:cds;Parent=Msa0546710-mRNA-1
chr4_1 CDS 38928826 38929227 38928826 ID=Msa0546710-mRNA-1:cds;Parent=Msa0546710-mRNA-1
chr4_1 three_prime_UTR 38929228 38929370 38929228 ID=Msa0546710-mRNA-1:three_prime_utr;Parent=Msa0546710-mRNA-1
Gene Sequence

>Msa0546710

ATGTCGTTATTGTTCTCTTTGTTCCTGGACTTGCAATCATCTACTGCCAAAATAAACTTTGACTACTACATGCTAGCAGAAACATGGCCACCAACTTTTTGTCTAAACAAATCATGTGTCCCTAATATACCTCAAAATTTCAGCATCCACGGATTGTGGCCGCAGAACAAGAGGAAGCCTAACCCATATCATTGCACAAATGAGACACTGGAGCCGCATGTGCTTGATCAATTGAAGTCTAGGCTTAATAAGGTTTGGCCGTCTTTAAAATTAAAAGTAAATAACTCTAAATTTTGGAATGATGAGTGGAAGAAACATGGTACTTGCTCCACGATGCCACCATTCGATTACTTCAAGCTTGCCCTAGATTTGTATGCCCTAAATGACATCAAAGATCTTCTTAATAAAACTTTAACACCTGGTGGATCGGCGACGAAAGCTGCCATCGAGAGTGCTATAAACTTAGGCACCGGATCTAACCCTAAGCTTCATTGTGATAATAAAAACTCTAGTAATTTGATGGAAGTTAGACTCTGCTTCAATACCACCAACAGGTATATAAGTTGTCCTAATACTGGTACAAATTGTGGAGCTACAATACATTTACCAGAAGTTATTAAATAA
Protein sequence

>Msa0546710

MSLLFSLFLDLQSSTAKINFDYYMLAETWPPTFCLNKSCVPNIPQNFSIHGLWPQNKRKPNPYHCTNETLEPHVLDQLKSRLNKVWPSLKLKVNNSKFWNDEWKKHGTCSTMPPFDYFKLALDLYALNDIKDLLNKTLTPGGSATKAAIESAINLGTGSNPKLHCDNKNSSNLMEVRLCFNTTNRYISCPNTGTNCGATIHLPEVIK*