AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0999350


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999350 MtrunA17_Chr6g0485481 54.386 57 26 0 2 58 77 133 2.76e-15 66.2
Msa0999350 MtrunA17_Chr6g0485491 49.123 57 29 0 2 58 37 93 1.44e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGTCAACCGAAGTTTCTT+TGG 0.278909 6_4:+81297076 Msa0999350:CDS
TGATATTCGAAGTGAGCATT+TGG 0.374908 6_4:+81298078 Msa0999350:CDS
TTTGGAAATTGACTGCCATC+TGG 0.383584 6_4:+81298096 Msa0999350:CDS
TTATTGATGCTTGTTTCAAA+GGG 0.392912 6_4:+81296995 Msa0999350:CDS
TTGTCAACCGAAGTTTCTTT+GGG 0.400068 6_4:+81297077 Msa0999350:CDS
TATAGATGTCTTAGACGAAA+TGG 0.402741 6_4:+81296948 Msa0999350:CDS
ATTATTGATGCTTGTTTCAA+AGG 0.437843 6_4:+81296994 Msa0999350:CDS
CGGTTGACAATTCATCAGTT+GGG 0.454673 6_4:-81297064 None:intergenic
TCGGTTGACAATTCATCAGT+TGG 0.476268 6_4:-81297065 None:intergenic
TTGGAAATTGACTGCCATCT+GGG 0.509277 6_4:+81298097 Msa0999350:CDS
GTCTTAGACGAAATGGCTGA+TGG 0.517792 6_4:+81296955 Msa0999350:CDS
TCTTAGACGAAATGGCTGAT+GGG 0.541606 6_4:+81296956 Msa0999350:CDS
GAACAACACAACTTTGTTGT+GGG 0.546320 6_4:-81297034 None:intergenic
GTCTTGTGCATGTGACATCA+AGG 0.559632 6_4:-81298039 None:intergenic
CAAAGAACCCAAAGAAACTT+CGG 0.563407 6_4:-81297084 None:intergenic
TTGATGCTTGTTTCAAAGGG+GGG 0.571541 6_4:+81296998 Msa0999350:CDS
TTCTTCAATATCCTCCCAGA+TGG 0.574708 6_4:-81298111 None:intergenic
TGAACAACACAACTTTGTTG+TGG 0.575855 6_4:-81297035 None:intergenic
ATTGATGCTTGTTTCAAAGG+GGG 0.586181 6_4:+81296997 Msa0999350:CDS
CTTAGACGAAATGGCTGATG+GGG 0.616658 6_4:+81296957 Msa0999350:CDS
TATTGATGCTTGTTTCAAAG+GGG 0.663412 6_4:+81296996 Msa0999350:CDS
GAAATTGACTGCCATCTGGG+AGG 0.678917 6_4:+81298100 Msa0999350:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTTAATATAAATGTTGTT+TGG - chr6_4:81297594-81297613 None:intergenic 15.0%
!! AACTTAATATAAATGTTGTT+TGG - chr6_4:81297594-81297613 None:intergenic 15.0%
!!! ATTCTTGATGCATTATTTTA+GGG - chr6_4:81297145-81297164 None:intergenic 20.0%
!!! AATTCTTGATGCATTATTTT+AGG - chr6_4:81297146-81297165 None:intergenic 20.0%
!!! CATTTGAGATTTCTTTAAAT+TGG + chr6_4:81297382-81297401 Msa0999350:intron 20.0%
!! TAGAAGATAAGTCAATAAAA+AGG - chr6_4:81297552-81297571 None:intergenic 20.0%
!!! AAAAAGTCACTTTTTAATTG+AGG - chr6_4:81297617-81297636 None:intergenic 20.0%
!!! AAAATATGACTTCTGAAATA+AGG + chr6_4:81297656-81297675 Msa0999350:intron 20.0%
!! ACATAAAATATGTGTTCATT+GGG - chr6_4:81297742-81297761 None:intergenic 20.0%
!! AACATAAAATATGTGTTCAT+TGG - chr6_4:81297743-81297762 None:intergenic 20.0%
!! TATGTTTCATAAATGTTGTT+TGG + chr6_4:81297757-81297776 Msa0999350:intron 20.0%
!!! AGTTTCTTTGTTATAACTAT+TGG + chr6_4:81297842-81297861 Msa0999350:intron 20.0%
!! ACATCAATTGAATTATTGTT+TGG + chr6_4:81297942-81297961 Msa0999350:intron 20.0%
!!! ATTCTTGATGCATTATTTTA+GGG - chr6_4:81297145-81297164 None:intergenic 20.0%
!!! AATTCTTGATGCATTATTTT+AGG - chr6_4:81297146-81297165 None:intergenic 20.0%
!!! CATTTGAGATTTCTTTAAAT+TGG + chr6_4:81297382-81297401 Msa0999350:intron 20.0%
!! TAGAAGATAAGTCAATAAAA+AGG - chr6_4:81297552-81297571 None:intergenic 20.0%
!!! AAAAAGTCACTTTTTAATTG+AGG - chr6_4:81297617-81297636 None:intergenic 20.0%
!!! AAAATATGACTTCTGAAATA+AGG + chr6_4:81297656-81297675 Msa0999350:intron 20.0%
!! ACATAAAATATGTGTTCATT+GGG - chr6_4:81297742-81297761 None:intergenic 20.0%
!! AACATAAAATATGTGTTCAT+TGG - chr6_4:81297743-81297762 None:intergenic 20.0%
!! TATGTTTCATAAATGTTGTT+TGG + chr6_4:81297757-81297776 Msa0999350:intron 20.0%
!!! AGTTTCTTTGTTATAACTAT+TGG + chr6_4:81297842-81297861 Msa0999350:intron 20.0%
!! ACATCAATTGAATTATTGTT+TGG + chr6_4:81297942-81297961 Msa0999350:intron 20.0%
! ATTATTGATGCTTGTTTCAA+AGG + chr6_4:81296994-81297013 Msa0999350:CDS 25.0%
! TTATTGATGCTTGTTTCAAA+GGG + chr6_4:81296995-81297014 Msa0999350:CDS 25.0%
! GACATGATTCAAAGAATATT+TGG + chr6_4:81297170-81297189 Msa0999350:intron 25.0%
!! CAAAGAATATTTGGTAATCA+TGG + chr6_4:81297179-81297198 Msa0999350:intron 25.0%
!!! TTTTTTGTTACAGTCTAGTA+AGG + chr6_4:81297415-81297434 Msa0999350:intron 25.0%
! CAAAATAAATTAAGCGAAGA+GGG - chr6_4:81297454-81297473 None:intergenic 25.0%
! TAATTCCTCAATTACAATCA+CGG - chr6_4:81297489-81297508 None:intergenic 25.0%
! CCATACAAATACTTAATTGA+TGG - chr6_4:81297900-81297919 None:intergenic 25.0%
! CCATCAATTAAGTATTTGTA+TGG + chr6_4:81297897-81297916 Msa0999350:intron 25.0%
! AGCATTCTATATTTGCTTAA+AGG + chr6_4:81298016-81298035 Msa0999350:intron 25.0%
! ATTATTGATGCTTGTTTCAA+AGG + chr6_4:81296994-81297013 Msa0999350:CDS 25.0%
! TTATTGATGCTTGTTTCAAA+GGG + chr6_4:81296995-81297014 Msa0999350:CDS 25.0%
! GACATGATTCAAAGAATATT+TGG + chr6_4:81297170-81297189 Msa0999350:intron 25.0%
!! CAAAGAATATTTGGTAATCA+TGG + chr6_4:81297179-81297198 Msa0999350:intron 25.0%
!!! TTTTTTGTTACAGTCTAGTA+AGG + chr6_4:81297415-81297434 Msa0999350:intron 25.0%
! CAAAATAAATTAAGCGAAGA+GGG - chr6_4:81297454-81297473 None:intergenic 25.0%
! TAATTCCTCAATTACAATCA+CGG - chr6_4:81297489-81297508 None:intergenic 25.0%
! CCATACAAATACTTAATTGA+TGG - chr6_4:81297900-81297919 None:intergenic 25.0%
! CCATCAATTAAGTATTTGTA+TGG + chr6_4:81297897-81297916 Msa0999350:intron 25.0%
! AGCATTCTATATTTGCTTAA+AGG + chr6_4:81298016-81298035 Msa0999350:intron 25.0%
TATAGATGTCTTAGACGAAA+TGG + chr6_4:81296948-81296967 Msa0999350:CDS 30.0%
TATTGATGCTTGTTTCAAAG+GGG + chr6_4:81296996-81297015 Msa0999350:CDS 30.0%
AACACACTTTACAAACACAT+TGG - chr6_4:81297114-81297133 None:intergenic 30.0%
! AGAATATTTGGTAATCATGG+AGG + chr6_4:81297182-81297201 Msa0999350:intron 30.0%
GCAAAATAAATTAAGCGAAG+AGG - chr6_4:81297455-81297474 None:intergenic 30.0%
GAGGAATTATTATACTATGC+TGG + chr6_4:81297500-81297519 Msa0999350:intron 30.0%
! TACTATGCTGGTAGATTTTT+AGG + chr6_4:81297512-81297531 Msa0999350:intron 30.0%
! ACTATGCTGGTAGATTTTTA+GGG + chr6_4:81297513-81297532 Msa0999350:intron 30.0%
AGATAAGTCAATAAAAAGGC+AGG - chr6_4:81297548-81297567 None:intergenic 30.0%
TTCTACTTCAAAGTAAAAGC+AGG + chr6_4:81297567-81297586 Msa0999350:intron 30.0%
TCTACTTCAAAGTAAAAGCA+GGG + chr6_4:81297568-81297587 Msa0999350:intron 30.0%
TCTACTTCAAAGTAAAAGCA+GGG - chr6_4:81297568-81297587 None:intergenic 30.0%
TTCTACTTCAAAGTAAAAGC+AGG - chr6_4:81297567-81297586 None:intergenic 30.0%
TATAGATGTCTTAGACGAAA+TGG + chr6_4:81296948-81296967 Msa0999350:CDS 30.0%
TATTGATGCTTGTTTCAAAG+GGG + chr6_4:81296996-81297015 Msa0999350:CDS 30.0%
AACACACTTTACAAACACAT+TGG - chr6_4:81297114-81297133 None:intergenic 30.0%
! AGAATATTTGGTAATCATGG+AGG + chr6_4:81297182-81297201 Msa0999350:intron 30.0%
GCAAAATAAATTAAGCGAAG+AGG - chr6_4:81297455-81297474 None:intergenic 30.0%
GAGGAATTATTATACTATGC+TGG + chr6_4:81297500-81297519 Msa0999350:intron 30.0%
! TACTATGCTGGTAGATTTTT+AGG + chr6_4:81297512-81297531 Msa0999350:intron 30.0%
! ACTATGCTGGTAGATTTTTA+GGG + chr6_4:81297513-81297532 Msa0999350:intron 30.0%
AGATAAGTCAATAAAAAGGC+AGG - chr6_4:81297548-81297567 None:intergenic 30.0%
TTCTACTTCAAAGTAAAAGC+AGG + chr6_4:81297567-81297586 Msa0999350:intron 30.0%
TCTACTTCAAAGTAAAAGCA+GGG + chr6_4:81297568-81297587 Msa0999350:intron 30.0%
TCTACTTCAAAGTAAAAGCA+GGG - chr6_4:81297568-81297587 None:intergenic 30.0%
TTCTACTTCAAAGTAAAAGC+AGG - chr6_4:81297567-81297586 None:intergenic 30.0%
ATTGATGCTTGTTTCAAAGG+GGG + chr6_4:81296997-81297016 Msa0999350:CDS 35.0%
! GAACAACACAACTTTGTTGT+GGG - chr6_4:81297037-81297056 None:intergenic 35.0%
TGAACAACACAACTTTGTTG+TGG - chr6_4:81297038-81297057 None:intergenic 35.0%
ATTGTCAACCGAAGTTTCTT+TGG + chr6_4:81297076-81297095 Msa0999350:CDS 35.0%
TTGTCAACCGAAGTTTCTTT+GGG + chr6_4:81297077-81297096 Msa0999350:CDS 35.0%
CAAAGAACCCAAAGAAACTT+CGG - chr6_4:81297087-81297106 None:intergenic 35.0%
!! TCAAGAGTATCATTACGTTC+AGG - chr6_4:81297218-81297237 None:intergenic 35.0%
AGCTAACAAAGTGAGAATTG+AGG + chr6_4:81297271-81297290 Msa0999350:intron 35.0%
ATGAGGTATGAGAACTCATA+TGG + chr6_4:81297319-81297338 Msa0999350:intron 35.0%
! AGAACTCATATGGTTTTCCT+GGG + chr6_4:81297329-81297348 Msa0999350:intron 35.0%
ATAAATTAAGCGAAGAGGGA+CGG - chr6_4:81297450-81297469 None:intergenic 35.0%
TACTTCCGTGATTGTAATTG+AGG + chr6_4:81297481-81297500 Msa0999350:intron 35.0%
!!! GTAGATTTTTAGGGTCTGTT+TGG + chr6_4:81297522-81297541 Msa0999350:intron 35.0%
! TTTGTATTCTGCTTCTGAAG+AGG - chr6_4:81297997-81298016 None:intergenic 35.0%
TGATATTCGAAGTGAGCATT+TGG + chr6_4:81298078-81298097 Msa0999350:CDS 35.0%
ATTGATGCTTGTTTCAAAGG+GGG + chr6_4:81296997-81297016 Msa0999350:CDS 35.0%
! GAACAACACAACTTTGTTGT+GGG - chr6_4:81297037-81297056 None:intergenic 35.0%
TGAACAACACAACTTTGTTG+TGG - chr6_4:81297038-81297057 None:intergenic 35.0%
ATTGTCAACCGAAGTTTCTT+TGG + chr6_4:81297076-81297095 Msa0999350:CDS 35.0%
TTGTCAACCGAAGTTTCTTT+GGG + chr6_4:81297077-81297096 Msa0999350:CDS 35.0%
CAAAGAACCCAAAGAAACTT+CGG - chr6_4:81297087-81297106 None:intergenic 35.0%
!! TCAAGAGTATCATTACGTTC+AGG - chr6_4:81297218-81297237 None:intergenic 35.0%
AGCTAACAAAGTGAGAATTG+AGG + chr6_4:81297271-81297290 Msa0999350:intron 35.0%
ATGAGGTATGAGAACTCATA+TGG + chr6_4:81297319-81297338 Msa0999350:intron 35.0%
! AGAACTCATATGGTTTTCCT+GGG + chr6_4:81297329-81297348 Msa0999350:intron 35.0%
ATAAATTAAGCGAAGAGGGA+CGG - chr6_4:81297450-81297469 None:intergenic 35.0%
TACTTCCGTGATTGTAATTG+AGG + chr6_4:81297481-81297500 Msa0999350:intron 35.0%
!!! GTAGATTTTTAGGGTCTGTT+TGG + chr6_4:81297522-81297541 Msa0999350:intron 35.0%
! TTTGTATTCTGCTTCTGAAG+AGG - chr6_4:81297997-81298016 None:intergenic 35.0%
TGATATTCGAAGTGAGCATT+TGG + chr6_4:81298078-81298097 Msa0999350:CDS 35.0%
TCTTAGACGAAATGGCTGAT+GGG + chr6_4:81296956-81296975 Msa0999350:CDS 40.0%
TTGATGCTTGTTTCAAAGGG+GGG + chr6_4:81296998-81297017 Msa0999350:CDS 40.0%
CGGTTGACAATTCATCAGTT+GGG - chr6_4:81297067-81297086 None:intergenic 40.0%
TCGGTTGACAATTCATCAGT+TGG - chr6_4:81297068-81297087 None:intergenic 40.0%
GTATCATTACGTTCAGGTAG+TGG - chr6_4:81297212-81297231 None:intergenic 40.0%
AGAGTGTAATCGTGTGAATG+AGG + chr6_4:81297302-81297321 Msa0999350:intron 40.0%
! GAGAACTCATATGGTTTTCC+TGG + chr6_4:81297328-81297347 Msa0999350:intron 40.0%
TGTGTTCATTGGGATTCGAA+TGG - chr6_4:81297732-81297751 None:intergenic 40.0%
! ACGTGAATCAGTCTGTTTCT+TGG - chr6_4:81297973-81297992 None:intergenic 40.0%
TTTGGAAATTGACTGCCATC+TGG + chr6_4:81298096-81298115 Msa0999350:CDS 40.0%
TTGGAAATTGACTGCCATCT+GGG + chr6_4:81298097-81298116 Msa0999350:CDS 40.0%
TTCTTCAATATCCTCCCAGA+TGG - chr6_4:81298114-81298133 None:intergenic 40.0%
TCTTAGACGAAATGGCTGAT+GGG + chr6_4:81296956-81296975 Msa0999350:CDS 40.0%
TTGATGCTTGTTTCAAAGGG+GGG + chr6_4:81296998-81297017 Msa0999350:CDS 40.0%
CGGTTGACAATTCATCAGTT+GGG - chr6_4:81297067-81297086 None:intergenic 40.0%
TCGGTTGACAATTCATCAGT+TGG - chr6_4:81297068-81297087 None:intergenic 40.0%
GTATCATTACGTTCAGGTAG+TGG - chr6_4:81297212-81297231 None:intergenic 40.0%
AGAGTGTAATCGTGTGAATG+AGG + chr6_4:81297302-81297321 Msa0999350:intron 40.0%
! GAGAACTCATATGGTTTTCC+TGG + chr6_4:81297328-81297347 Msa0999350:intron 40.0%
TGTGTTCATTGGGATTCGAA+TGG - chr6_4:81297732-81297751 None:intergenic 40.0%
! ACGTGAATCAGTCTGTTTCT+TGG - chr6_4:81297973-81297992 None:intergenic 40.0%
TTTGGAAATTGACTGCCATC+TGG + chr6_4:81298096-81298115 Msa0999350:CDS 40.0%
TTGGAAATTGACTGCCATCT+GGG + chr6_4:81298097-81298116 Msa0999350:CDS 40.0%
TTCTTCAATATCCTCCCAGA+TGG - chr6_4:81298114-81298133 None:intergenic 40.0%
GTCTTAGACGAAATGGCTGA+TGG + chr6_4:81296955-81296974 Msa0999350:CDS 45.0%
CTTAGACGAAATGGCTGATG+GGG + chr6_4:81296957-81296976 Msa0999350:CDS 45.0%
! CATTACGTTCAGGTAGTGGT+AGG - chr6_4:81297208-81297227 None:intergenic 45.0%
CACGCGTAACTGATAGAAGT+AGG - chr6_4:81297690-81297709 None:intergenic 45.0%
GTTCATTGGGATTCGAATGG+TGG - chr6_4:81297729-81297748 None:intergenic 45.0%
GTCTTGTGCATGTGACATCA+AGG - chr6_4:81298042-81298061 None:intergenic 45.0%
GTCTTAGACGAAATGGCTGA+TGG + chr6_4:81296955-81296974 Msa0999350:CDS 45.0%
CTTAGACGAAATGGCTGATG+GGG + chr6_4:81296957-81296976 Msa0999350:CDS 45.0%
! CATTACGTTCAGGTAGTGGT+AGG - chr6_4:81297208-81297227 None:intergenic 45.0%
CACGCGTAACTGATAGAAGT+AGG - chr6_4:81297690-81297709 None:intergenic 45.0%
GTTCATTGGGATTCGAATGG+TGG - chr6_4:81297729-81297748 None:intergenic 45.0%
GTCTTGTGCATGTGACATCA+AGG - chr6_4:81298042-81298061 None:intergenic 45.0%
! CCTTACTTGCTAGACTTCCC+AGG - chr6_4:81297349-81297368 None:intergenic 50.0%
CCTGGGAAGTCTAGCAAGTA+AGG + chr6_4:81297346-81297365 Msa0999350:intron 50.0%
GAAATTGACTGCCATCTGGG+AGG + chr6_4:81298100-81298119 Msa0999350:CDS 50.0%
! CCTTACTTGCTAGACTTCCC+AGG - chr6_4:81297349-81297368 None:intergenic 50.0%
CCTGGGAAGTCTAGCAAGTA+AGG + chr6_4:81297346-81297365 Msa0999350:intron 50.0%
GAAATTGACTGCCATCTGGG+AGG + chr6_4:81298100-81298119 Msa0999350:CDS 50.0%
! GGGATTCGAATGGTGGAGCA+GGG - chr6_4:81297722-81297741 None:intergenic 55.0%
! TGGGATTCGAATGGTGGAGC+AGG - chr6_4:81297723-81297742 None:intergenic 55.0%
! GGGATTCGAATGGTGGAGCA+GGG - chr6_4:81297722-81297741 None:intergenic 55.0%
! TGGGATTCGAATGGTGGAGC+AGG - chr6_4:81297723-81297742 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 81296895 81298136 81296895 ID=Msa0999350;Name=Msa0999350
chr6_4 mRNA 81296895 81298136 81296895 ID=Msa0999350-mRNA-1;Parent=Msa0999350;Name=Msa0999350-mRNA-1;_AED=0.46;_eAED=0.73;_QI=0|0|0|1|0|0|3|0|103
chr6_4 exon 81296895 81297098 81296895 ID=Msa0999350-mRNA-1:exon:15548;Parent=Msa0999350-mRNA-1
chr6_4 exon 81297428 81297436 81297428 ID=Msa0999350-mRNA-1:exon:15549;Parent=Msa0999350-mRNA-1
chr6_4 exon 81298038 81298136 81298038 ID=Msa0999350-mRNA-1:exon:15550;Parent=Msa0999350-mRNA-1
chr6_4 CDS 81296895 81297098 81296895 ID=Msa0999350-mRNA-1:cds;Parent=Msa0999350-mRNA-1
chr6_4 CDS 81297428 81297436 81297428 ID=Msa0999350-mRNA-1:cds;Parent=Msa0999350-mRNA-1
chr6_4 CDS 81298038 81298136 81298038 ID=Msa0999350-mRNA-1:cds;Parent=Msa0999350-mRNA-1
Gene Sequence

>Msa0999350

ATGCATATATTAATTTTATTAGTTTGCGACGTGACTTGTACAAGTTATGAATTTATAGATGTCTTAGACGAAATGGCTGATGGGGTCAAGACAACTTTCATTATTGATGCTTGTTTCAAAGGGGGGTTTATAGAACTAGCCCACAACAAAGTTGTGTTGTTCACATCATCCCAACTGATGAATTGTCAACCGAAGTTTCTTTGGTCTAGTAAGGCCTTGATGTCACATGCACAAGACTCGTTGCGTTATATTGTGATATTCGAAGTGAGCATTTGGAAATTGACTGCCATCTGGGAGGATATTGAAGAATAA

Protein sequence

>Msa0999350

MHILILLVCDVTCTSYEFIDVLDEMADGVKTTFIIDACFKGGFIELAHNKVVLFTSSQLMNCQPKFLWSSKALMSHAQDSLRYIVIFEVSIWKLTAIWEDIEE*