AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0999400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999400 MtrunA17_Chr6g0485481 54.386 57 26 0 2 58 77 133 2.76e-15 66.2
Msa0999400 MtrunA17_Chr6g0485491 49.123 57 29 0 2 58 37 93 1.44e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGTCAACCGAAGTTTCTT+TGG 0.278909 6_4:+81356182 Msa0999400:CDS
TGATATTCGAAGTGAGCATT+TGG 0.374908 6_4:+81357184 Msa0999400:CDS
TTTGGAAATTGACTGCCATC+TGG 0.383584 6_4:+81357202 Msa0999400:CDS
TTATTGATGCTTGTTTCAAA+GGG 0.392912 6_4:+81356101 Msa0999400:CDS
TTGTCAACCGAAGTTTCTTT+GGG 0.400068 6_4:+81356183 Msa0999400:CDS
TATAGATGTCTTAGACGAAA+TGG 0.402741 6_4:+81356054 Msa0999400:CDS
ATTATTGATGCTTGTTTCAA+AGG 0.437843 6_4:+81356100 Msa0999400:CDS
CGGTTGACAATTCATCAGTT+GGG 0.454673 6_4:-81356170 None:intergenic
TCGGTTGACAATTCATCAGT+TGG 0.476268 6_4:-81356171 None:intergenic
TTGGAAATTGACTGCCATCT+GGG 0.509277 6_4:+81357203 Msa0999400:CDS
GTCTTAGACGAAATGGCTGA+TGG 0.517792 6_4:+81356061 Msa0999400:CDS
TCTTAGACGAAATGGCTGAT+GGG 0.541606 6_4:+81356062 Msa0999400:CDS
GAACAACACAACTTTGTTGT+GGG 0.546320 6_4:-81356140 None:intergenic
GTCTTGTGCATGTGACATCA+AGG 0.559632 6_4:-81357145 None:intergenic
CAAAGAACCCAAAGAAACTT+CGG 0.563407 6_4:-81356190 None:intergenic
TTGATGCTTGTTTCAAAGGG+GGG 0.571541 6_4:+81356104 Msa0999400:CDS
TTCTTCAATATCCTCCCAGA+TGG 0.574708 6_4:-81357217 None:intergenic
TGAACAACACAACTTTGTTG+TGG 0.575855 6_4:-81356141 None:intergenic
ATTGATGCTTGTTTCAAAGG+GGG 0.586181 6_4:+81356103 Msa0999400:CDS
CTTAGACGAAATGGCTGATG+GGG 0.616658 6_4:+81356063 Msa0999400:CDS
TATTGATGCTTGTTTCAAAG+GGG 0.663412 6_4:+81356102 Msa0999400:CDS
GAAATTGACTGCCATCTGGG+AGG 0.678917 6_4:+81357206 Msa0999400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTTAATATAAATGTTGTT+TGG - chr6_4:81356700-81356719 None:intergenic 15.0%
!! AACTTAATATAAATGTTGTT+TGG - chr6_4:81356700-81356719 None:intergenic 15.0%
!!! ATTCTTGATGCATTATTTTA+GGG - chr6_4:81356251-81356270 None:intergenic 20.0%
!!! AATTCTTGATGCATTATTTT+AGG - chr6_4:81356252-81356271 None:intergenic 20.0%
!!! CATTTGAGATTTCTTTAAAT+TGG + chr6_4:81356488-81356507 Msa0999400:intron 20.0%
!! TAGAAGATAAGTCAATAAAA+AGG - chr6_4:81356658-81356677 None:intergenic 20.0%
!!! AAAAAGTCACTTTTTAATTG+AGG - chr6_4:81356723-81356742 None:intergenic 20.0%
!!! AAAATATGACTTCTGAAATA+AGG + chr6_4:81356762-81356781 Msa0999400:intron 20.0%
!! ACATAAAATATGTGTTCATT+GGG - chr6_4:81356848-81356867 None:intergenic 20.0%
!! AACATAAAATATGTGTTCAT+TGG - chr6_4:81356849-81356868 None:intergenic 20.0%
!! TATGTTTCATAAATGTTGTT+TGG + chr6_4:81356863-81356882 Msa0999400:intron 20.0%
!!! AGTTTCTTTGTTATAACTAT+TGG + chr6_4:81356948-81356967 Msa0999400:intron 20.0%
!! ACATCAATTGAATTATTGTT+TGG + chr6_4:81357048-81357067 Msa0999400:intron 20.0%
!!! ATTCTTGATGCATTATTTTA+GGG - chr6_4:81356251-81356270 None:intergenic 20.0%
!!! AATTCTTGATGCATTATTTT+AGG - chr6_4:81356252-81356271 None:intergenic 20.0%
!!! CATTTGAGATTTCTTTAAAT+TGG + chr6_4:81356488-81356507 Msa0999400:intron 20.0%
!! TAGAAGATAAGTCAATAAAA+AGG - chr6_4:81356658-81356677 None:intergenic 20.0%
!!! AAAAAGTCACTTTTTAATTG+AGG - chr6_4:81356723-81356742 None:intergenic 20.0%
!!! AAAATATGACTTCTGAAATA+AGG + chr6_4:81356762-81356781 Msa0999400:intron 20.0%
!! ACATAAAATATGTGTTCATT+GGG - chr6_4:81356848-81356867 None:intergenic 20.0%
!! AACATAAAATATGTGTTCAT+TGG - chr6_4:81356849-81356868 None:intergenic 20.0%
!! TATGTTTCATAAATGTTGTT+TGG + chr6_4:81356863-81356882 Msa0999400:intron 20.0%
!!! AGTTTCTTTGTTATAACTAT+TGG + chr6_4:81356948-81356967 Msa0999400:intron 20.0%
!! ACATCAATTGAATTATTGTT+TGG + chr6_4:81357048-81357067 Msa0999400:intron 20.0%
! ATTATTGATGCTTGTTTCAA+AGG + chr6_4:81356100-81356119 Msa0999400:CDS 25.0%
! TTATTGATGCTTGTTTCAAA+GGG + chr6_4:81356101-81356120 Msa0999400:CDS 25.0%
! GACATGATTCAAAGAATATT+TGG + chr6_4:81356276-81356295 Msa0999400:intron 25.0%
!! CAAAGAATATTTGGTAATCA+TGG + chr6_4:81356285-81356304 Msa0999400:intron 25.0%
!!! TTTTTTGTTACAGTCTAGTA+AGG + chr6_4:81356521-81356540 Msa0999400:intron 25.0%
! CAAAATAAATTAAGCGAAGA+GGG - chr6_4:81356560-81356579 None:intergenic 25.0%
! TAATTCCTCAATTACAATCA+CGG - chr6_4:81356595-81356614 None:intergenic 25.0%
! CCATACAAATACTTAATTGA+TGG - chr6_4:81357006-81357025 None:intergenic 25.0%
! CCATCAATTAAGTATTTGTA+TGG + chr6_4:81357003-81357022 Msa0999400:intron 25.0%
! AGCATTCTATATTTGCTTAA+AGG + chr6_4:81357122-81357141 Msa0999400:intron 25.0%
! ATTATTGATGCTTGTTTCAA+AGG + chr6_4:81356100-81356119 Msa0999400:CDS 25.0%
! TTATTGATGCTTGTTTCAAA+GGG + chr6_4:81356101-81356120 Msa0999400:CDS 25.0%
! GACATGATTCAAAGAATATT+TGG + chr6_4:81356276-81356295 Msa0999400:intron 25.0%
!! CAAAGAATATTTGGTAATCA+TGG + chr6_4:81356285-81356304 Msa0999400:intron 25.0%
!!! TTTTTTGTTACAGTCTAGTA+AGG + chr6_4:81356521-81356540 Msa0999400:intron 25.0%
! CAAAATAAATTAAGCGAAGA+GGG - chr6_4:81356560-81356579 None:intergenic 25.0%
! TAATTCCTCAATTACAATCA+CGG - chr6_4:81356595-81356614 None:intergenic 25.0%
! CCATACAAATACTTAATTGA+TGG - chr6_4:81357006-81357025 None:intergenic 25.0%
! CCATCAATTAAGTATTTGTA+TGG + chr6_4:81357003-81357022 Msa0999400:intron 25.0%
! AGCATTCTATATTTGCTTAA+AGG + chr6_4:81357122-81357141 Msa0999400:intron 25.0%
TATAGATGTCTTAGACGAAA+TGG + chr6_4:81356054-81356073 Msa0999400:CDS 30.0%
TATTGATGCTTGTTTCAAAG+GGG + chr6_4:81356102-81356121 Msa0999400:CDS 30.0%
AACACACTTTACAAACACAT+TGG - chr6_4:81356220-81356239 None:intergenic 30.0%
! AGAATATTTGGTAATCATGG+AGG + chr6_4:81356288-81356307 Msa0999400:intron 30.0%
GCAAAATAAATTAAGCGAAG+AGG - chr6_4:81356561-81356580 None:intergenic 30.0%
GAGGAATTATTATACTATGC+TGG + chr6_4:81356606-81356625 Msa0999400:intron 30.0%
! TACTATGCTGGTAGATTTTT+AGG + chr6_4:81356618-81356637 Msa0999400:intron 30.0%
! ACTATGCTGGTAGATTTTTA+GGG + chr6_4:81356619-81356638 Msa0999400:intron 30.0%
AGATAAGTCAATAAAAAGGC+AGG - chr6_4:81356654-81356673 None:intergenic 30.0%
TTCTACTTCAAAGTAAAAGC+AGG + chr6_4:81356673-81356692 Msa0999400:intron 30.0%
TCTACTTCAAAGTAAAAGCA+GGG + chr6_4:81356674-81356693 Msa0999400:intron 30.0%
TCTACTTCAAAGTAAAAGCA+GGG - chr6_4:81356674-81356693 None:intergenic 30.0%
TTCTACTTCAAAGTAAAAGC+AGG - chr6_4:81356673-81356692 None:intergenic 30.0%
TATAGATGTCTTAGACGAAA+TGG + chr6_4:81356054-81356073 Msa0999400:CDS 30.0%
TATTGATGCTTGTTTCAAAG+GGG + chr6_4:81356102-81356121 Msa0999400:CDS 30.0%
AACACACTTTACAAACACAT+TGG - chr6_4:81356220-81356239 None:intergenic 30.0%
! AGAATATTTGGTAATCATGG+AGG + chr6_4:81356288-81356307 Msa0999400:intron 30.0%
GCAAAATAAATTAAGCGAAG+AGG - chr6_4:81356561-81356580 None:intergenic 30.0%
GAGGAATTATTATACTATGC+TGG + chr6_4:81356606-81356625 Msa0999400:intron 30.0%
! TACTATGCTGGTAGATTTTT+AGG + chr6_4:81356618-81356637 Msa0999400:intron 30.0%
! ACTATGCTGGTAGATTTTTA+GGG + chr6_4:81356619-81356638 Msa0999400:intron 30.0%
AGATAAGTCAATAAAAAGGC+AGG - chr6_4:81356654-81356673 None:intergenic 30.0%
TTCTACTTCAAAGTAAAAGC+AGG + chr6_4:81356673-81356692 Msa0999400:intron 30.0%
TCTACTTCAAAGTAAAAGCA+GGG + chr6_4:81356674-81356693 Msa0999400:intron 30.0%
TCTACTTCAAAGTAAAAGCA+GGG - chr6_4:81356674-81356693 None:intergenic 30.0%
TTCTACTTCAAAGTAAAAGC+AGG - chr6_4:81356673-81356692 None:intergenic 30.0%
ATTGATGCTTGTTTCAAAGG+GGG + chr6_4:81356103-81356122 Msa0999400:CDS 35.0%
! GAACAACACAACTTTGTTGT+GGG - chr6_4:81356143-81356162 None:intergenic 35.0%
TGAACAACACAACTTTGTTG+TGG - chr6_4:81356144-81356163 None:intergenic 35.0%
ATTGTCAACCGAAGTTTCTT+TGG + chr6_4:81356182-81356201 Msa0999400:CDS 35.0%
TTGTCAACCGAAGTTTCTTT+GGG + chr6_4:81356183-81356202 Msa0999400:CDS 35.0%
CAAAGAACCCAAAGAAACTT+CGG - chr6_4:81356193-81356212 None:intergenic 35.0%
!! TCAAGAGTATCATTACGTTC+AGG - chr6_4:81356324-81356343 None:intergenic 35.0%
AGCTAACAAAGTGAGAATTG+AGG + chr6_4:81356377-81356396 Msa0999400:intron 35.0%
ATGAGGTATGAGAACTCATA+TGG + chr6_4:81356425-81356444 Msa0999400:intron 35.0%
! AGAACTCATATGGTTTTCCT+GGG + chr6_4:81356435-81356454 Msa0999400:intron 35.0%
ATAAATTAAGCGAAGAGGGA+CGG - chr6_4:81356556-81356575 None:intergenic 35.0%
TACTTCCGTGATTGTAATTG+AGG + chr6_4:81356587-81356606 Msa0999400:intron 35.0%
!!! GTAGATTTTTAGGGTCTGTT+TGG + chr6_4:81356628-81356647 Msa0999400:intron 35.0%
! TTTGTATTCTGCTTCTGAAG+AGG - chr6_4:81357103-81357122 None:intergenic 35.0%
TGATATTCGAAGTGAGCATT+TGG + chr6_4:81357184-81357203 Msa0999400:CDS 35.0%
ATTGATGCTTGTTTCAAAGG+GGG + chr6_4:81356103-81356122 Msa0999400:CDS 35.0%
! GAACAACACAACTTTGTTGT+GGG - chr6_4:81356143-81356162 None:intergenic 35.0%
TGAACAACACAACTTTGTTG+TGG - chr6_4:81356144-81356163 None:intergenic 35.0%
ATTGTCAACCGAAGTTTCTT+TGG + chr6_4:81356182-81356201 Msa0999400:CDS 35.0%
TTGTCAACCGAAGTTTCTTT+GGG + chr6_4:81356183-81356202 Msa0999400:CDS 35.0%
CAAAGAACCCAAAGAAACTT+CGG - chr6_4:81356193-81356212 None:intergenic 35.0%
!! TCAAGAGTATCATTACGTTC+AGG - chr6_4:81356324-81356343 None:intergenic 35.0%
AGCTAACAAAGTGAGAATTG+AGG + chr6_4:81356377-81356396 Msa0999400:intron 35.0%
ATGAGGTATGAGAACTCATA+TGG + chr6_4:81356425-81356444 Msa0999400:intron 35.0%
! AGAACTCATATGGTTTTCCT+GGG + chr6_4:81356435-81356454 Msa0999400:intron 35.0%
ATAAATTAAGCGAAGAGGGA+CGG - chr6_4:81356556-81356575 None:intergenic 35.0%
TACTTCCGTGATTGTAATTG+AGG + chr6_4:81356587-81356606 Msa0999400:intron 35.0%
!!! GTAGATTTTTAGGGTCTGTT+TGG + chr6_4:81356628-81356647 Msa0999400:intron 35.0%
! TTTGTATTCTGCTTCTGAAG+AGG - chr6_4:81357103-81357122 None:intergenic 35.0%
TGATATTCGAAGTGAGCATT+TGG + chr6_4:81357184-81357203 Msa0999400:CDS 35.0%
TCTTAGACGAAATGGCTGAT+GGG + chr6_4:81356062-81356081 Msa0999400:CDS 40.0%
TTGATGCTTGTTTCAAAGGG+GGG + chr6_4:81356104-81356123 Msa0999400:CDS 40.0%
CGGTTGACAATTCATCAGTT+GGG - chr6_4:81356173-81356192 None:intergenic 40.0%
TCGGTTGACAATTCATCAGT+TGG - chr6_4:81356174-81356193 None:intergenic 40.0%
GTATCATTACGTTCAGGTAG+TGG - chr6_4:81356318-81356337 None:intergenic 40.0%
AGAGTGTAATCGTGTGAATG+AGG + chr6_4:81356408-81356427 Msa0999400:intron 40.0%
! GAGAACTCATATGGTTTTCC+TGG + chr6_4:81356434-81356453 Msa0999400:intron 40.0%
TGTGTTCATTGGGATTCGAA+TGG - chr6_4:81356838-81356857 None:intergenic 40.0%
! ACGTGAATCAGTCTGTTTCT+TGG - chr6_4:81357079-81357098 None:intergenic 40.0%
TTTGGAAATTGACTGCCATC+TGG + chr6_4:81357202-81357221 Msa0999400:CDS 40.0%
TTGGAAATTGACTGCCATCT+GGG + chr6_4:81357203-81357222 Msa0999400:CDS 40.0%
TTCTTCAATATCCTCCCAGA+TGG - chr6_4:81357220-81357239 None:intergenic 40.0%
TCTTAGACGAAATGGCTGAT+GGG + chr6_4:81356062-81356081 Msa0999400:CDS 40.0%
TTGATGCTTGTTTCAAAGGG+GGG + chr6_4:81356104-81356123 Msa0999400:CDS 40.0%
CGGTTGACAATTCATCAGTT+GGG - chr6_4:81356173-81356192 None:intergenic 40.0%
TCGGTTGACAATTCATCAGT+TGG - chr6_4:81356174-81356193 None:intergenic 40.0%
GTATCATTACGTTCAGGTAG+TGG - chr6_4:81356318-81356337 None:intergenic 40.0%
AGAGTGTAATCGTGTGAATG+AGG + chr6_4:81356408-81356427 Msa0999400:intron 40.0%
! GAGAACTCATATGGTTTTCC+TGG + chr6_4:81356434-81356453 Msa0999400:intron 40.0%
TGTGTTCATTGGGATTCGAA+TGG - chr6_4:81356838-81356857 None:intergenic 40.0%
! ACGTGAATCAGTCTGTTTCT+TGG - chr6_4:81357079-81357098 None:intergenic 40.0%
TTTGGAAATTGACTGCCATC+TGG + chr6_4:81357202-81357221 Msa0999400:CDS 40.0%
TTGGAAATTGACTGCCATCT+GGG + chr6_4:81357203-81357222 Msa0999400:CDS 40.0%
TTCTTCAATATCCTCCCAGA+TGG - chr6_4:81357220-81357239 None:intergenic 40.0%
GTCTTAGACGAAATGGCTGA+TGG + chr6_4:81356061-81356080 Msa0999400:CDS 45.0%
CTTAGACGAAATGGCTGATG+GGG + chr6_4:81356063-81356082 Msa0999400:CDS 45.0%
! CATTACGTTCAGGTAGTGGT+AGG - chr6_4:81356314-81356333 None:intergenic 45.0%
CACGCGTAACTGATAGAAGT+AGG - chr6_4:81356796-81356815 None:intergenic 45.0%
GTTCATTGGGATTCGAATGG+TGG - chr6_4:81356835-81356854 None:intergenic 45.0%
GTCTTGTGCATGTGACATCA+AGG - chr6_4:81357148-81357167 None:intergenic 45.0%
GTCTTAGACGAAATGGCTGA+TGG + chr6_4:81356061-81356080 Msa0999400:CDS 45.0%
CTTAGACGAAATGGCTGATG+GGG + chr6_4:81356063-81356082 Msa0999400:CDS 45.0%
! CATTACGTTCAGGTAGTGGT+AGG - chr6_4:81356314-81356333 None:intergenic 45.0%
CACGCGTAACTGATAGAAGT+AGG - chr6_4:81356796-81356815 None:intergenic 45.0%
GTTCATTGGGATTCGAATGG+TGG - chr6_4:81356835-81356854 None:intergenic 45.0%
GTCTTGTGCATGTGACATCA+AGG - chr6_4:81357148-81357167 None:intergenic 45.0%
! CCTTACTTGCTAGACTTCCC+AGG - chr6_4:81356455-81356474 None:intergenic 50.0%
CCTGGGAAGTCTAGCAAGTA+AGG + chr6_4:81356452-81356471 Msa0999400:intron 50.0%
GAAATTGACTGCCATCTGGG+AGG + chr6_4:81357206-81357225 Msa0999400:CDS 50.0%
! CCTTACTTGCTAGACTTCCC+AGG - chr6_4:81356455-81356474 None:intergenic 50.0%
CCTGGGAAGTCTAGCAAGTA+AGG + chr6_4:81356452-81356471 Msa0999400:intron 50.0%
GAAATTGACTGCCATCTGGG+AGG + chr6_4:81357206-81357225 Msa0999400:CDS 50.0%
! GGGATTCGAATGGTGGAGCA+GGG - chr6_4:81356828-81356847 None:intergenic 55.0%
! TGGGATTCGAATGGTGGAGC+AGG - chr6_4:81356829-81356848 None:intergenic 55.0%
! GGGATTCGAATGGTGGAGCA+GGG - chr6_4:81356828-81356847 None:intergenic 55.0%
! TGGGATTCGAATGGTGGAGC+AGG - chr6_4:81356829-81356848 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 81356001 81357242 81356001 ID=Msa0999400;Name=Msa0999400
chr6_4 mRNA 81356001 81357242 81356001 ID=Msa0999400-mRNA-1;Parent=Msa0999400;Name=Msa0999400-mRNA-1;_AED=0.46;_eAED=0.73;_QI=0|0|0|1|0|0|3|0|103
chr6_4 exon 81356001 81356204 81356001 ID=Msa0999400-mRNA-1:exon:15551;Parent=Msa0999400-mRNA-1
chr6_4 exon 81356534 81356542 81356534 ID=Msa0999400-mRNA-1:exon:15552;Parent=Msa0999400-mRNA-1
chr6_4 exon 81357144 81357242 81357144 ID=Msa0999400-mRNA-1:exon:15553;Parent=Msa0999400-mRNA-1
chr6_4 CDS 81356001 81356204 81356001 ID=Msa0999400-mRNA-1:cds;Parent=Msa0999400-mRNA-1
chr6_4 CDS 81356534 81356542 81356534 ID=Msa0999400-mRNA-1:cds;Parent=Msa0999400-mRNA-1
chr6_4 CDS 81357144 81357242 81357144 ID=Msa0999400-mRNA-1:cds;Parent=Msa0999400-mRNA-1
Gene Sequence

>Msa0999400

ATGCATATATTAATTTTATTAGTTTGCGACGTGACTTGTACAAGTTATGAATTTATAGATGTCTTAGACGAAATGGCTGATGGGGTCAAGACAACTTTCATTATTGATGCTTGTTTCAAAGGGGGGTTTATAGAACTAGCCCACAACAAAGTTGTGTTGTTCACATCATCCCAACTGATGAATTGTCAACCGAAGTTTCTTTGGTCTAGTAAGGCCTTGATGTCACATGCACAAGACTCGTTGCGTTATATTGTGATATTCGAAGTGAGCATTTGGAAATTGACTGCCATCTGGGAGGATATTGAAGAATAA

Protein sequence

>Msa0999400

MHILILLVCDVTCTSYEFIDVLDEMADGVKTTFIIDACFKGGFIELAHNKVVLFTSSQLMNCQPKFLWSSKALMSHAQDSLRYIVIFEVSIWKLTAIWEDIEE*