Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0053690 | Msa0798090 | 0.873420 | 1.491054e-67 | -8.615850e-47 |
| Msa0053700 | Msa0798090 | 0.859232 | 4.768321e-63 | -8.615850e-47 |
| Msa0798090 | Msa0798100 | 0.870901 | 1.027610e-66 | -8.615850e-47 |
| Msa0798090 | Msa0799480 | 0.840615 | 7.814164e-58 | -8.615850e-47 |
| Msa0798090 | Msa1067070 | 0.843679 | 1.208152e-58 | -8.615850e-47 |
| Msa0798090 | Msa1253400 | 0.848271 | 6.816827e-60 | -8.615850e-47 |
| Msa0798090 | Msa1279590 | 0.834630 | 2.681666e-56 | -8.615850e-47 |
| Msa0798090 | Msa1289300 | 0.875371 | 3.249484e-68 | -8.615850e-47 |
| Msa0798090 | Msa1297600 | 0.875490 | 2.957517e-68 | -8.615850e-47 |
| Msa0164370 | Msa0798090 | 0.881568 | 2.154612e-70 | -8.615850e-47 |
| Msa0364990 | Msa0798090 | 0.853619 | 2.120656e-61 | -8.615850e-47 |
| Msa0414020 | Msa0798090 | 0.845231 | 4.620334e-59 | -8.615850e-47 |
| Msa0431770 | Msa0798090 | 0.802363 | 5.813615e-49 | -8.615850e-47 |
| Msa0337500 | Msa0798090 | 0.852589 | 4.180676e-61 | -8.615850e-47 |
| Msa0337510 | Msa0798090 | 0.865347 | 6.305749e-65 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0798090 | MtrunA17_Chr2g0328391 | 45.833 | 96 | 5 | 1 | 7 | 55 | 254 | 349 | 1.02e-15 | 70.1 |
| Msa0798090 | MtrunA17_Chr4g0073551 | 88.889 | 27 | 3 | 0 | 52 | 78 | 121 | 147 | 1.01e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGACTATGACAATGTAAT+TGG | 0.287106 | 5_3:+10669618 | Msa0798090:CDS |
| TTTCTGCTTACTTTCAGATA+TGG | 0.359746 | 5_3:-10669648 | None:intergenic |
| CAGGTTCTACATGGTGCATC+TGG | 0.398474 | 5_3:+10668780 | None:intergenic |
| ACACAGGTTGGTGAACTTGA+TGG | 0.468419 | 5_3:+10668901 | Msa0798090:CDS |
| AGGTGATGCATCACAGAGAA+TGG | 0.535230 | 5_3:+10669074 | Msa0798090:intron |
| GTCGTTGCGTGCTGAACAAA+AGG | 0.535793 | 5_3:+10668855 | Msa0798090:CDS |
| TTGCGTGCTGAACAAAAGGA+TGG | 0.589695 | 5_3:+10668859 | Msa0798090:CDS |
| TGGTGAACTTGATGGAGGGA+AGG | 0.605242 | 5_3:+10668909 | Msa0798090:CDS |
| GAATGCGATTGAAGCGCGCA+TGG | 0.609128 | 5_3:+10669110 | Msa0798090:CDS |
| CAGGTTGGTGAACTTGATGG+AGG | 0.633962 | 5_3:+10668904 | Msa0798090:CDS |
| ACAAAAGGATGGATCAGTGG+TGG | 0.638622 | 5_3:+10668870 | Msa0798090:CDS |
| AGGTTGGTGAACTTGATGGA+GGG | 0.640702 | 5_3:+10668905 | Msa0798090:CDS |
| AGTGGTGGAGAGTAGAACAC+AGG | 0.692386 | 5_3:+10668885 | Msa0798090:CDS |
| TGAACAAAAGGATGGATCAG+TGG | 0.692488 | 5_3:+10668867 | Msa0798090:CDS |
| GTGGAGAGTAGAACACAGGT+TGG | 0.695448 | 5_3:+10668889 | Msa0798090:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATATGTTGTTTTGTAAAT+AGG | + | chr5_3:10669499-10669518 | Msa0798090:intron | 15.0% |
| !!! | ATATATGTTGTTTTGTAAAT+AGG | + | chr5_3:10669499-10669518 | Msa0798090:intron | 15.0% |
| !! | CATATATAATATTTGTGTAC+TGG | - | chr5_3:10669148-10669167 | None:intergenic | 20.0% |
| !!! | AATGTGCAAATTTTTTGAAA+GGG | - | chr5_3:10669233-10669252 | None:intergenic | 20.0% |
| !!! | AAATGTGCAAATTTTTTGAA+AGG | - | chr5_3:10669234-10669253 | None:intergenic | 20.0% |
| !!! | TTTAAAAAACATGGCAATTT+TGG | - | chr5_3:10669326-10669345 | None:intergenic | 20.0% |
| !!! | ACATACATCTTTAAAAAACA+TGG | - | chr5_3:10669335-10669354 | None:intergenic | 20.0% |
| !!! | GTTTTTTAAAGATGTATGTT+TGG | + | chr5_3:10669336-10669355 | Msa0798090:intron | 20.0% |
| !! | CATATATAATATTTGTGTAC+TGG | - | chr5_3:10669148-10669167 | None:intergenic | 20.0% |
| !!! | AATGTGCAAATTTTTTGAAA+GGG | - | chr5_3:10669233-10669252 | None:intergenic | 20.0% |
| !!! | AAATGTGCAAATTTTTTGAA+AGG | - | chr5_3:10669234-10669253 | None:intergenic | 20.0% |
| !!! | TTTAAAAAACATGGCAATTT+TGG | - | chr5_3:10669326-10669345 | None:intergenic | 20.0% |
| !!! | ACATACATCTTTAAAAAACA+TGG | - | chr5_3:10669335-10669354 | None:intergenic | 20.0% |
| !!! | GTTTTTTAAAGATGTATGTT+TGG | + | chr5_3:10669336-10669355 | Msa0798090:intron | 20.0% |
| ! | GTACAGAAATTTGAATTCAT+TGG | + | chr5_3:10668806-10668825 | Msa0798090:intron | 25.0% |
| !! | TTTTCTTTTTGTAAGTTGCA+TGG | - | chr5_3:10668832-10668851 | None:intergenic | 25.0% |
| ! | TTAAAGATGTATGTTTGGAT+TGG | + | chr5_3:10669341-10669360 | Msa0798090:intron | 25.0% |
| ! | ATAATTGTGTTTGTTCATGA+AGG | + | chr5_3:10669404-10669423 | Msa0798090:intron | 25.0% |
| ! | TAGGAAACTTCTATTTGATT+AGG | + | chr5_3:10669518-10669537 | Msa0798090:intron | 25.0% |
| ! | GTTAGAGATGAGAAAATTAA+GGG | - | chr5_3:10669561-10669580 | None:intergenic | 25.0% |
| ! | AGTTAGAGATGAGAAAATTA+AGG | - | chr5_3:10669562-10669581 | None:intergenic | 25.0% |
| !!! | TCTCTAACTTTTGTTTATTG+TGG | + | chr5_3:10669573-10669592 | Msa0798090:intron | 25.0% |
| ! | GTACAGAAATTTGAATTCAT+TGG | + | chr5_3:10668806-10668825 | Msa0798090:intron | 25.0% |
| !! | TTTTCTTTTTGTAAGTTGCA+TGG | - | chr5_3:10668832-10668851 | None:intergenic | 25.0% |
| ! | TTAAAGATGTATGTTTGGAT+TGG | + | chr5_3:10669341-10669360 | Msa0798090:intron | 25.0% |
| ! | ATAATTGTGTTTGTTCATGA+AGG | + | chr5_3:10669404-10669423 | Msa0798090:intron | 25.0% |
| ! | TAGGAAACTTCTATTTGATT+AGG | + | chr5_3:10669518-10669537 | Msa0798090:intron | 25.0% |
| ! | GTTAGAGATGAGAAAATTAA+GGG | - | chr5_3:10669561-10669580 | None:intergenic | 25.0% |
| ! | AGTTAGAGATGAGAAAATTA+AGG | - | chr5_3:10669562-10669581 | None:intergenic | 25.0% |
| !!! | TCTCTAACTTTTGTTTATTG+TGG | + | chr5_3:10669573-10669592 | Msa0798090:intron | 25.0% |
| ATGAATTGTTGAAGCAGAAT+AGG | + | chr5_3:10669034-10669053 | Msa0798090:intron | 30.0% | |
| ! | GAGTGTTTAGATTTGTTTGT+AGG | + | chr5_3:10669368-10669387 | Msa0798090:intron | 30.0% |
| !! | AAGCTAAGTTGATGTATCAT+AGG | - | chr5_3:10669444-10669463 | None:intergenic | 30.0% |
| AGTGACTATGACAATGTAAT+TGG | + | chr5_3:10669618-10669637 | Msa0798090:CDS | 30.0% | |
| TTTCTGCTTACTTTCAGATA+TGG | - | chr5_3:10669651-10669670 | None:intergenic | 30.0% | |
| ATGAATTGTTGAAGCAGAAT+AGG | + | chr5_3:10669034-10669053 | Msa0798090:intron | 30.0% | |
| ! | GAGTGTTTAGATTTGTTTGT+AGG | + | chr5_3:10669368-10669387 | Msa0798090:intron | 30.0% |
| !! | AAGCTAAGTTGATGTATCAT+AGG | - | chr5_3:10669444-10669463 | None:intergenic | 30.0% |
| AGTGACTATGACAATGTAAT+TGG | + | chr5_3:10669618-10669637 | Msa0798090:CDS | 30.0% | |
| TTTCTGCTTACTTTCAGATA+TGG | - | chr5_3:10669651-10669670 | None:intergenic | 30.0% | |
| ! | CAGCAGCATTAAACATCTTT+TGG | - | chr5_3:10669173-10669192 | None:intergenic | 35.0% |
| TTGTGTTTGTTCATGAAGGT+TGG | + | chr5_3:10669408-10669427 | Msa0798090:intron | 35.0% | |
| ! | CAGCAGCATTAAACATCTTT+TGG | - | chr5_3:10669173-10669192 | None:intergenic | 35.0% |
| TTGTGTTTGTTCATGAAGGT+TGG | + | chr5_3:10669408-10669427 | Msa0798090:intron | 35.0% | |
| TGAACAAAAGGATGGATCAG+TGG | + | chr5_3:10668867-10668886 | Msa0798090:CDS | 40.0% | |
| TTGTGCACACAGAACCAAAA+GGG | - | chr5_3:10668983-10669002 | None:intergenic | 40.0% | |
| CTGTGTCTGCTAATGACAAT+AGG | + | chr5_3:10669004-10669023 | Msa0798090:intron | 40.0% | |
| GGCAAGCAGAGATAAAAACT+TGG | - | chr5_3:10669212-10669231 | None:intergenic | 40.0% | |
| TGAACAAAAGGATGGATCAG+TGG | + | chr5_3:10668867-10668886 | Msa0798090:CDS | 40.0% | |
| TTGTGCACACAGAACCAAAA+GGG | - | chr5_3:10668983-10669002 | None:intergenic | 40.0% | |
| CTGTGTCTGCTAATGACAAT+AGG | + | chr5_3:10669004-10669023 | Msa0798090:intron | 40.0% | |
| GGCAAGCAGAGATAAAAACT+TGG | - | chr5_3:10669212-10669231 | None:intergenic | 40.0% | |
| TTGCGTGCTGAACAAAAGGA+TGG | + | chr5_3:10668859-10668878 | Msa0798090:CDS | 45.0% | |
| ACAAAAGGATGGATCAGTGG+TGG | + | chr5_3:10668870-10668889 | Msa0798090:CDS | 45.0% | |
| !! | ACACAGGTTGGTGAACTTGA+TGG | + | chr5_3:10668901-10668920 | Msa0798090:CDS | 45.0% |
| !! | AGGTTGGTGAACTTGATGGA+GGG | + | chr5_3:10668905-10668924 | Msa0798090:CDS | 45.0% |
| AAGGCACGTTCGAAGAAACA+AGG | + | chr5_3:10668928-10668947 | Msa0798090:intron | 45.0% | |
| AGGCACGTTCGAAGAAACAA+GGG | + | chr5_3:10668929-10668948 | Msa0798090:intron | 45.0% | |
| GAAGAAACAAGGGAGGAAGA+GGG | + | chr5_3:10668939-10668958 | Msa0798090:intron | 45.0% | |
| AGAGGGATGTTGTGGATTTG+AGG | + | chr5_3:10668956-10668975 | Msa0798090:intron | 45.0% | |
| ! | TGTGGATTTGAGGACCCTTT+TGG | + | chr5_3:10668966-10668985 | Msa0798090:intron | 45.0% |
| CTTGTGCACACAGAACCAAA+AGG | - | chr5_3:10668984-10669003 | None:intergenic | 45.0% | |
| !! | AGGAAGCATTCTTGTCCTTC+AGG | + | chr5_3:10669054-10669073 | Msa0798090:intron | 45.0% |
| AGGTGATGCATCACAGAGAA+TGG | + | chr5_3:10669074-10669093 | Msa0798090:intron | 45.0% | |
| TTGCGTGCTGAACAAAAGGA+TGG | + | chr5_3:10668859-10668878 | Msa0798090:CDS | 45.0% | |
| ACAAAAGGATGGATCAGTGG+TGG | + | chr5_3:10668870-10668889 | Msa0798090:CDS | 45.0% | |
| !! | ACACAGGTTGGTGAACTTGA+TGG | + | chr5_3:10668901-10668920 | Msa0798090:CDS | 45.0% |
| !! | AGGTTGGTGAACTTGATGGA+GGG | + | chr5_3:10668905-10668924 | Msa0798090:CDS | 45.0% |
| AAGGCACGTTCGAAGAAACA+AGG | + | chr5_3:10668928-10668947 | Msa0798090:intron | 45.0% | |
| AGGCACGTTCGAAGAAACAA+GGG | + | chr5_3:10668929-10668948 | Msa0798090:intron | 45.0% | |
| GAAGAAACAAGGGAGGAAGA+GGG | + | chr5_3:10668939-10668958 | Msa0798090:intron | 45.0% | |
| AGAGGGATGTTGTGGATTTG+AGG | + | chr5_3:10668956-10668975 | Msa0798090:intron | 45.0% | |
| ! | TGTGGATTTGAGGACCCTTT+TGG | + | chr5_3:10668966-10668985 | Msa0798090:intron | 45.0% |
| CTTGTGCACACAGAACCAAA+AGG | - | chr5_3:10668984-10669003 | None:intergenic | 45.0% | |
| !! | AGGAAGCATTCTTGTCCTTC+AGG | + | chr5_3:10669054-10669073 | Msa0798090:intron | 45.0% |
| AGGTGATGCATCACAGAGAA+TGG | + | chr5_3:10669074-10669093 | Msa0798090:intron | 45.0% | |
| !! | TAAAAAAAAATATTGAAATA+TGG | - | chr5_3:10669467-10669486 | None:intergenic | 5.0% |
| !!! | TCAATATTTTTTTTTATTTA+TGG | + | chr5_3:10669471-10669490 | Msa0798090:intron | 5.0% |
| !! | TAAAAAAAAATATTGAAATA+TGG | - | chr5_3:10669467-10669486 | None:intergenic | 5.0% |
| !!! | TCAATATTTTTTTTTATTTA+TGG | + | chr5_3:10669471-10669490 | Msa0798090:intron | 5.0% |
| GTCGTTGCGTGCTGAACAAA+AGG | + | chr5_3:10668855-10668874 | Msa0798090:CDS | 50.0% | |
| ! | AGTGGTGGAGAGTAGAACAC+AGG | + | chr5_3:10668885-10668904 | Msa0798090:CDS | 50.0% |
| GTGGAGAGTAGAACACAGGT+TGG | + | chr5_3:10668889-10668908 | Msa0798090:CDS | 50.0% | |
| !! | CAGGTTGGTGAACTTGATGG+AGG | + | chr5_3:10668904-10668923 | Msa0798090:CDS | 50.0% |
| TGGTGAACTTGATGGAGGGA+AGG | + | chr5_3:10668909-10668928 | Msa0798090:CDS | 50.0% | |
| CACGTTCGAAGAAACAAGGG+AGG | + | chr5_3:10668932-10668951 | Msa0798090:intron | 50.0% | |
| CGAAGAAACAAGGGAGGAAG+AGG | + | chr5_3:10668938-10668957 | Msa0798090:intron | 50.0% | |
| CTCTGTGATGCATCACCTGA+AGG | - | chr5_3:10669072-10669091 | None:intergenic | 50.0% | |
| GTCGTTGCGTGCTGAACAAA+AGG | + | chr5_3:10668855-10668874 | Msa0798090:CDS | 50.0% | |
| ! | AGTGGTGGAGAGTAGAACAC+AGG | + | chr5_3:10668885-10668904 | Msa0798090:CDS | 50.0% |
| GTGGAGAGTAGAACACAGGT+TGG | + | chr5_3:10668889-10668908 | Msa0798090:CDS | 50.0% | |
| !! | CAGGTTGGTGAACTTGATGG+AGG | + | chr5_3:10668904-10668923 | Msa0798090:CDS | 50.0% |
| TGGTGAACTTGATGGAGGGA+AGG | + | chr5_3:10668909-10668928 | Msa0798090:CDS | 50.0% | |
| CACGTTCGAAGAAACAAGGG+AGG | + | chr5_3:10668932-10668951 | Msa0798090:intron | 50.0% | |
| CGAAGAAACAAGGGAGGAAG+AGG | + | chr5_3:10668938-10668957 | Msa0798090:intron | 50.0% | |
| CTCTGTGATGCATCACCTGA+AGG | - | chr5_3:10669072-10669091 | None:intergenic | 50.0% | |
| AGGGAGGAAGAGGGATGTTG+TGG | + | chr5_3:10668948-10668967 | Msa0798090:intron | 55.0% | |
| GAATGCGATTGAAGCGCGCA+TGG | + | chr5_3:10669110-10669129 | Msa0798090:CDS | 55.0% | |
| AGGGAGGAAGAGGGATGTTG+TGG | + | chr5_3:10668948-10668967 | Msa0798090:intron | 55.0% | |
| GAATGCGATTGAAGCGCGCA+TGG | + | chr5_3:10669110-10669129 | Msa0798090:CDS | 55.0% | |
| GCGATTGAAGCGCGCATGGT+TGG | + | chr5_3:10669114-10669133 | Msa0798090:intron | 60.0% | |
| GCGATTGAAGCGCGCATGGT+TGG | + | chr5_3:10669114-10669133 | Msa0798090:intron | 60.0% | |
| ! | GAAGCGCGCATGGTTGGTGC+TGG | + | chr5_3:10669120-10669139 | Msa0798090:intron | 65.0% |
| ! | GAAGCGCGCATGGTTGGTGC+TGG | + | chr5_3:10669120-10669139 | Msa0798090:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 10668783 | 10669680 | 10668783 | ID=Msa0798090;Name=Msa0798090 |
| chr5_3 | mRNA | 10668783 | 10669680 | 10668783 | ID=Msa0798090-mRNA-1;Parent=Msa0798090;Name=Msa0798090-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|0.33|0|0.75|0|0.5|4|0|79 |
| chr5_3 | exon | 10668783 | 10668801 | 10668783 | ID=Msa0798090-mRNA-1:exon:4420;Parent=Msa0798090-mRNA-1 |
| chr5_3 | exon | 10668848 | 10668934 | 10668848 | ID=Msa0798090-mRNA-1:exon:4421;Parent=Msa0798090-mRNA-1 |
| chr5_3 | exon | 10669076 | 10669131 | 10669076 | ID=Msa0798090-mRNA-1:exon:4422;Parent=Msa0798090-mRNA-1 |
| chr5_3 | exon | 10669603 | 10669680 | 10669603 | ID=Msa0798090-mRNA-1:exon:4423;Parent=Msa0798090-mRNA-1 |
| chr5_3 | CDS | 10668783 | 10668801 | 10668783 | ID=Msa0798090-mRNA-1:cds;Parent=Msa0798090-mRNA-1 |
| chr5_3 | CDS | 10668848 | 10668934 | 10668848 | ID=Msa0798090-mRNA-1:cds;Parent=Msa0798090-mRNA-1 |
| chr5_3 | CDS | 10669076 | 10669131 | 10669076 | ID=Msa0798090-mRNA-1:cds;Parent=Msa0798090-mRNA-1 |
| chr5_3 | CDS | 10669603 | 10669680 | 10669603 | ID=Msa0798090-mRNA-1:cds;Parent=Msa0798090-mRNA-1 |
| Gene Sequence |
| Protein sequence |
