AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0053690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0053690 MtrunA17_Chr2g0323051 50.847 118 48 4 21 138 211 318 5.65e-26 102
Msa0053690 MtrunA17_Chr3g0140681 95.556 45 2 0 1 45 209 253 1.44e-23 95.1
Msa0053690 MtrunA17_Chr4g0023601 56.164 73 29 1 29 98 135 207 2.95e-20 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0053690 AT2G32290.1 50.000 90 43 2 45 134 219 306 2.23e-20 87.0
Msa0053690 AT4G15210.1 62.963 54 20 0 45 98 156 209 7.29e-19 82.8
Msa0053690 AT4G15210.3 62.963 54 20 0 45 98 103 156 2.47e-18 80.9
Msa0053690 AT4G15210.4 62.963 54 20 0 45 98 103 156 2.47e-18 80.9
Msa0053690 AT4G15210.2 62.963 54 20 0 45 98 156 209 2.48e-18 80.9
Msa0053690 AT4G00490.1 49.091 55 28 0 45 99 236 290 1.79e-11 61.6

Find 56 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATGTTTATAGATTAATT+AGG 0.166605 1_2:-8204523 Msa0053690:three_prime_UTR
TATGATAATTTAATCAAAAT+TGG 0.267934 1_2:-8204452 Msa0053690:three_prime_UTR
TCTCGTCTTTCGCGGTTTCT+TGG 0.271778 1_2:-8205799 Msa0053690:CDS
GACTGGGTATTTCTTGGTAT+TGG 0.274714 1_2:-8205141 Msa0053690:CDS
ATATGTTTATAGATTAATTA+GGG 0.278241 1_2:-8204522 Msa0053690:three_prime_UTR
CTTCAAATAGTCAGCCATTC+TGG 0.282618 1_2:+8204671 None:intergenic
AAGCTTAATAGCAGCCTTAC+TGG 0.305599 1_2:-8204495 Msa0053690:three_prime_UTR
GAAGAGTTATAGAGAGAATA+TGG 0.309022 1_2:-8205256 Msa0053690:CDS
GTTATGTGGATTTCACTCTT+TGG 0.332758 1_2:-8205833 Msa0053690:CDS
TCGCGGTTTCTTGGTAAAAC+AGG 0.335407 1_2:-8205790 Msa0053690:CDS
TGCCTAGACTGGGTATTTCT+TGG 0.342837 1_2:-8205147 Msa0053690:CDS
AATCCTGGACTCACCGCTTA+TGG 0.358745 1_2:+8204775 None:intergenic
CCGCTTATGGTGACATCATA+AGG 0.359764 1_2:+8204788 None:intergenic
TCCCTCTCATGCATGATATC+TGG 0.374941 1_2:+8204745 None:intergenic
CTTTAGGTTCCTTTGTTATG+TGG 0.393628 1_2:-8205847 None:intergenic
ATAAGGGCTGGTGCACCTAC+TGG 0.427494 1_2:+8204805 None:intergenic
ATAGTCAGCCATTCTGGCTC+TGG 0.443426 1_2:+8204677 None:intergenic
TATATCTATATATATGTTCA+AGG 0.447915 1_2:+8204587 None:intergenic
ATATCTGGCCTCACTAATCC+TGG 0.482839 1_2:+8204760 None:intergenic
AAACAGCATCTCGTCTTTCG+CGG 0.485483 1_2:-8205807 Msa0053690:CDS
GAGGTAGGACTTGGCCCTGC+TGG 0.486945 1_2:-8205198 Msa0053690:CDS
CTTATAAGCGTGTACGTGGC+AGG 0.500665 1_2:-8204946 Msa0053690:intron
TCACCATAAGCGGTGAGTCC+AGG 0.505916 1_2:-8204778 Msa0053690:CDS
CGCTTATGGTGACATCATAA+GGG 0.505983 1_2:+8204789 None:intergenic
CGCGGTTTCTTGGTAAAACA+GGG 0.510516 1_2:-8205789 Msa0053690:CDS
ACCTCCTCTTCTTCTCCAGA+TGG 0.522445 1_2:-8205731 Msa0053690:intron
AAAGCTTAATGTAAGAATCT+CGG 0.527749 1_2:+8205000 None:intergenic
TTGAGATTCCAGAGCCAGAA+TGG 0.530384 1_2:-8204685 Msa0053690:three_prime_UTR
TTGTCAAATATGAACCAGTA+AGG 0.531051 1_2:+8204481 None:intergenic
AGATATCTAAGTTCTCCAGC+AGG 0.532605 1_2:+8205183 None:intergenic
AAATTGGAAATGCAGGGGAC+AGG 0.536511 1_2:-8204831 Msa0053690:intron
TATGGTGACATCATAAGGGC+TGG 0.556208 1_2:+8204793 None:intergenic
AATGGCTGACTATTTGAAGC+AGG 0.562267 1_2:-8204667 Msa0053690:three_prime_UTR
CGAACCATCTGGAGAAGAAG+AGG 0.566975 1_2:+8205727 None:intergenic
ATGTTTATAGATTAATTAGG+GGG 0.570090 1_2:-8204520 Msa0053690:three_prime_UTR
GCTTTACTCATGATGCTGAA+AGG 0.577029 1_2:-8204982 Msa0053690:CDS
CCTTATGATGTCACCATAAG+CGG 0.579231 1_2:-8204788 Msa0053690:CDS
TACCAAGAAATACCCAGTCT+AGG 0.594121 1_2:+8205145 None:intergenic
GCCAGATATCATGCATGAGA+GGG 0.594778 1_2:-8204746 Msa0053690:exon
GGCCAGATATCATGCATGAG+AGG 0.594930 1_2:-8204747 Msa0053690:exon
ATAGACATTGAGGTAGGACT+TGG 0.597655 1_2:-8205207 Msa0053690:CDS
TGAGCTACTGATAGACATTG+AGG 0.599509 1_2:-8205217 Msa0053690:CDS
CTCTGGAATCTCAATCCTCG+AGG 0.601459 1_2:+8204694 None:intergenic
CAGTCTTATAAGCGTGTACG+TGG 0.609552 1_2:-8204950 Msa0053690:CDS
TATTTGAAGCAGGATGGCAA+AGG 0.629381 1_2:-8204657 Msa0053690:three_prime_UTR
GATATCTAAGTTCTCCAGCA+GGG 0.632849 1_2:+8205184 None:intergenic
TCTGGAATCTCAATCCTCGA+GGG 0.635537 1_2:+8204695 None:intergenic
CTACTGATAGACATTGAGGT+AGG 0.639731 1_2:-8205213 Msa0053690:CDS
GCTGACTATTTGAAGCAGGA+TGG 0.643683 1_2:-8204663 Msa0053690:three_prime_UTR
ACCATCTGGAGAAGAAGAGG+AGG 0.646740 1_2:+8205730 None:intergenic
TATGTTTATAGATTAATTAG+GGG 0.649576 1_2:-8204521 Msa0053690:three_prime_UTR
CGGTGAGTCCAGGATTAGTG+AGG 0.671978 1_2:-8204768 Msa0053690:CDS
GCAGGGGACAGGCTTCCAGT+AGG 0.671994 1_2:-8204820 Msa0053690:intron
GAGTGAAATCCACATAACAA+AGG 0.672556 1_2:+8205838 None:intergenic
CAATCCTCGAGGGATCTCTG+AGG 0.696479 1_2:+8204705 None:intergenic
ATTTCCTCAGAGATCCCTCG+AGG 0.709622 1_2:-8204709 Msa0053690:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATGTTTATAGATTAATT+AGG - chr1_2:8205770-8205789 Msa0053690:CDS 10.0%
!! ATATGTTTATAGATTAATTA+GGG - chr1_2:8205771-8205790 Msa0053690:CDS 10.0%
!! TATGATAATTTAATCAAAAT+TGG - chr1_2:8205841-8205860 Msa0053690:CDS 10.0%
!! ATGATAATTTAATCAAAATT+GGG - chr1_2:8205842-8205861 Msa0053690:CDS 10.0%
!! TATATGTTTATAGATTAATT+AGG - chr1_2:8205770-8205789 Msa0053690:CDS 10.0%
!! ATATGTTTATAGATTAATTA+GGG - chr1_2:8205771-8205790 Msa0053690:CDS 10.0%
!! TTACATGAATTTAATATCTT+TGG + chr1_2:8204597-8204616 None:intergenic 15.0%
!! AAAGATATTAAATTCATGTA+AGG - chr1_2:8204596-8204615 Msa0053690:three_prime_UTR 15.0%
!! AAAGAAGTAATAATAAAAAG+TGG + chr1_2:8204912-8204931 None:intergenic 15.0%
!!! CATAACAAATTTTAGTAAAA+TGG + chr1_2:8205413-8205432 None:intergenic 15.0%
!! TGGAATATTTATATGAAAAT+TGG - chr1_2:8205446-8205465 Msa0053690:intron 15.0%
!! TATATCTATATATATGTTCA+AGG + chr1_2:8205709-8205728 None:intergenic 15.0%
!! TATGTTTATAGATTAATTAG+GGG - chr1_2:8205772-8205791 Msa0053690:CDS 15.0%
!! TTACATGAATTTAATATCTT+TGG + chr1_2:8204597-8204616 None:intergenic 15.0%
!! AAAGATATTAAATTCATGTA+AGG - chr1_2:8204596-8204615 Msa0053690:three_prime_UTR 15.0%
!! AAAGAAGTAATAATAAAAAG+TGG + chr1_2:8204912-8204931 None:intergenic 15.0%
!!! CATAACAAATTTTAGTAAAA+TGG + chr1_2:8205413-8205432 None:intergenic 15.0%
!! TGGAATATTTATATGAAAAT+TGG - chr1_2:8205446-8205465 Msa0053690:intron 15.0%
!! TATATCTATATATATGTTCA+AGG + chr1_2:8205709-8205728 None:intergenic 15.0%
!! TATGTTTATAGATTAATTAG+GGG - chr1_2:8205772-8205791 Msa0053690:CDS 15.0%
!!! AAGCATTTATTTTTATCTGA+TGG - chr1_2:8204527-8204546 Msa0053690:three_prime_UTR 20.0%
!! TATTATATCTCTTTCTCTAT+AGG - chr1_2:8204696-8204715 Msa0053690:three_prime_UTR 20.0%
!! TGAACTAAAACAAAAGAATA+CGG + chr1_2:8204775-8204794 None:intergenic 20.0%
!! TGTTATGTAATGTTTAATGT+TGG - chr1_2:8205426-8205445 Msa0053690:intron 20.0%
!! ATGTTTATAGATTAATTAGG+GGG - chr1_2:8205773-8205792 Msa0053690:CDS 20.0%
!!! AAGCATTTATTTTTATCTGA+TGG - chr1_2:8204527-8204546 Msa0053690:three_prime_UTR 20.0%
!! TATTATATCTCTTTCTCTAT+AGG - chr1_2:8204696-8204715 Msa0053690:three_prime_UTR 20.0%
!! TGAACTAAAACAAAAGAATA+CGG + chr1_2:8204775-8204794 None:intergenic 20.0%
!! TGTTATGTAATGTTTAATGT+TGG - chr1_2:8205426-8205445 Msa0053690:intron 20.0%
!! ATGTTTATAGATTAATTAGG+GGG - chr1_2:8205773-8205792 Msa0053690:CDS 20.0%
!! TATGTTGTTTGTTCATGATT+TGG + chr1_2:8204671-8204690 None:intergenic 25.0%
! GATAGTAATAGAATATGCAA+AGG + chr1_2:8204808-8204827 None:intergenic 25.0%
!!! TTCAAGTTTTGCTTATGTTT+AGG + chr1_2:8204876-8204895 None:intergenic 25.0%
! AAAGCTTAATGTAAGAATCT+CGG + chr1_2:8205296-8205315 None:intergenic 25.0%
! TATATGAAAATTGGAAATGC+AGG - chr1_2:8205455-8205474 Msa0053690:intron 25.0%
! ATATGAAAATTGGAAATGCA+GGG - chr1_2:8205456-8205475 Msa0053690:intron 25.0%
!! TATGTTGTTTGTTCATGATT+TGG + chr1_2:8204671-8204690 None:intergenic 25.0%
! GATAGTAATAGAATATGCAA+AGG + chr1_2:8204808-8204827 None:intergenic 25.0%
!!! TTCAAGTTTTGCTTATGTTT+AGG + chr1_2:8204876-8204895 None:intergenic 25.0%
! AAAGCTTAATGTAAGAATCT+CGG + chr1_2:8205296-8205315 None:intergenic 25.0%
! TATATGAAAATTGGAAATGC+AGG - chr1_2:8205455-8205474 Msa0053690:intron 25.0%
! ATATGAAAATTGGAAATGCA+GGG - chr1_2:8205456-8205475 Msa0053690:intron 25.0%
! GATTTGGTTTCTAAACCATA+AGG + chr1_2:8204655-8204674 None:intergenic 30.0%
TCATTTAAACTTACAGACAG+TGG + chr1_2:8204842-8204861 None:intergenic 30.0%
GAAGAGTTATAGAGAGAATA+TGG - chr1_2:8205037-8205056 Msa0053690:intron 30.0%
TTTAGTAAAATGGCAAGTCA+TGG + chr1_2:8205403-8205422 None:intergenic 30.0%
TATGAAAATTGGAAATGCAG+GGG - chr1_2:8205457-8205476 Msa0053690:intron 30.0%
!! CTCTGAGGAAATTTTGTATT+TGG + chr1_2:8205576-8205595 None:intergenic 30.0%
TGAAGAAACTGATGAAAAAG+AGG - chr1_2:8205674-8205693 Msa0053690:intron 30.0%
GAAGAAACTGATGAAAAAGA+GGG - chr1_2:8205675-8205694 Msa0053690:intron 30.0%
TTGTCAAATATGAACCAGTA+AGG + chr1_2:8205815-8205834 None:intergenic 30.0%
! GATTTGGTTTCTAAACCATA+AGG + chr1_2:8204655-8204674 None:intergenic 30.0%
TCATTTAAACTTACAGACAG+TGG + chr1_2:8204842-8204861 None:intergenic 30.0%
GAAGAGTTATAGAGAGAATA+TGG - chr1_2:8205037-8205056 Msa0053690:intron 30.0%
TTTAGTAAAATGGCAAGTCA+TGG + chr1_2:8205403-8205422 None:intergenic 30.0%
TATGAAAATTGGAAATGCAG+GGG - chr1_2:8205457-8205476 Msa0053690:intron 30.0%
!! CTCTGAGGAAATTTTGTATT+TGG + chr1_2:8205576-8205595 None:intergenic 30.0%
TGAAGAAACTGATGAAAAAG+AGG - chr1_2:8205674-8205693 Msa0053690:intron 30.0%
GAAGAAACTGATGAAAAAGA+GGG - chr1_2:8205675-8205694 Msa0053690:intron 30.0%
TTGTCAAATATGAACCAGTA+AGG + chr1_2:8205815-8205834 None:intergenic 30.0%
GAGTGAAATCCACATAACAA+AGG + chr1_2:8204458-8204477 None:intergenic 35.0%
GTTATGTGGATTTCACTCTT+TGG - chr1_2:8204460-8204479 Msa0053690:three_prime_UTR 35.0%
! TGATTTTCGCAACTTCCTTA+TGG - chr1_2:8204637-8204656 Msa0053690:three_prime_UTR 35.0%
TTCTCTATAGGTCTGCTTTA+CGG - chr1_2:8204708-8204727 Msa0053690:three_prime_UTR 35.0%
GTCGTAACGAATATTTGCAT+AGG + chr1_2:8204753-8204772 None:intergenic 35.0%
TTTCCCTTAGTTCCCTTAAT+TGG - chr1_2:8204985-8205004 Msa0053690:CDS 35.0%
ACACCAATTAAGGGAACTAA+GGG + chr1_2:8204991-8205010 None:intergenic 35.0%
AACACCAATTAAGGGAACTA+AGG + chr1_2:8204992-8205011 None:intergenic 35.0%
CTGCATCAAACACCAATTAA+GGG + chr1_2:8205000-8205019 None:intergenic 35.0%
TCTGCATCAAACACCAATTA+AGG + chr1_2:8205001-8205020 None:intergenic 35.0%
!! TTTTTCAGAATGCCTAGACT+GGG - chr1_2:8205136-8205155 Msa0053690:CDS 35.0%
TGAACATATATGCATACACG+TGG + chr1_2:8205230-8205249 None:intergenic 35.0%
CGTGTATGCATATATGTTCA+TGG - chr1_2:8205230-8205249 Msa0053690:CDS 35.0%
TGTATCCTGCATATTGTTTC+AGG - chr1_2:8205258-8205277 Msa0053690:CDS 35.0%
GTATCCTGCATATTGTTTCA+GGG - chr1_2:8205259-8205278 Msa0053690:CDS 35.0%
GAAATTGTTCCATCATACCT+CGG + chr1_2:8205377-8205396 None:intergenic 35.0%
GAGTGAAATCCACATAACAA+AGG + chr1_2:8204458-8204477 None:intergenic 35.0%
GTTATGTGGATTTCACTCTT+TGG - chr1_2:8204460-8204479 Msa0053690:three_prime_UTR 35.0%
! TGATTTTCGCAACTTCCTTA+TGG - chr1_2:8204637-8204656 Msa0053690:three_prime_UTR 35.0%
TTCTCTATAGGTCTGCTTTA+CGG - chr1_2:8204708-8204727 Msa0053690:three_prime_UTR 35.0%
GTCGTAACGAATATTTGCAT+AGG + chr1_2:8204753-8204772 None:intergenic 35.0%
TTTCCCTTAGTTCCCTTAAT+TGG - chr1_2:8204985-8205004 Msa0053690:CDS 35.0%
ACACCAATTAAGGGAACTAA+GGG + chr1_2:8204991-8205010 None:intergenic 35.0%
AACACCAATTAAGGGAACTA+AGG + chr1_2:8204992-8205011 None:intergenic 35.0%
CTGCATCAAACACCAATTAA+GGG + chr1_2:8205000-8205019 None:intergenic 35.0%
TCTGCATCAAACACCAATTA+AGG + chr1_2:8205001-8205020 None:intergenic 35.0%
!! TTTTTCAGAATGCCTAGACT+GGG - chr1_2:8205136-8205155 Msa0053690:CDS 35.0%
TGAACATATATGCATACACG+TGG + chr1_2:8205230-8205249 None:intergenic 35.0%
CGTGTATGCATATATGTTCA+TGG - chr1_2:8205230-8205249 Msa0053690:CDS 35.0%
TGTATCCTGCATATTGTTTC+AGG - chr1_2:8205258-8205277 Msa0053690:CDS 35.0%
GTATCCTGCATATTGTTTCA+GGG - chr1_2:8205259-8205278 Msa0053690:CDS 35.0%
GAAATTGTTCCATCATACCT+CGG + chr1_2:8205377-8205396 None:intergenic 35.0%
GAATATTTGCATAGGCAACG+CGG + chr1_2:8204745-8204764 None:intergenic 40.0%
TGAGCTACTGATAGACATTG+AGG - chr1_2:8205076-8205095 Msa0053690:intron 40.0%
CTACTGATAGACATTGAGGT+AGG - chr1_2:8205080-8205099 Msa0053690:intron 40.0%
ATAGACATTGAGGTAGGACT+TGG - chr1_2:8205086-8205105 Msa0053690:intron 40.0%
GATATCTAAGTTCTCCAGCA+GGG + chr1_2:8205112-8205131 None:intergenic 40.0%
AGATATCTAAGTTCTCCAGC+AGG + chr1_2:8205113-8205132 None:intergenic 40.0%
!!! CTTTTTCAGAATGCCTAGAC+TGG - chr1_2:8205135-8205154 Msa0053690:CDS 40.0%
TACCAAGAAATACCCAGTCT+AGG + chr1_2:8205151-8205170 None:intergenic 40.0%
GACTGGGTATTTCTTGGTAT+TGG - chr1_2:8205152-8205171 Msa0053690:CDS 40.0%
GCTTTACTCATGATGCTGAA+AGG - chr1_2:8205311-8205330 Msa0053690:intron 40.0%
CGCTTATGGTGACATCATAA+GGG + chr1_2:8205507-8205526 None:intergenic 40.0%
CCTTATGATGTCACCATAAG+CGG - chr1_2:8205505-8205524 Msa0053690:intron 40.0%
CTTCAAATAGTCAGCCATTC+TGG + chr1_2:8205625-8205644 None:intergenic 40.0%
! AATGGCTGACTATTTGAAGC+AGG - chr1_2:8205626-8205645 Msa0053690:intron 40.0%
! TATTTGAAGCAGGATGGCAA+AGG - chr1_2:8205636-8205655 Msa0053690:intron 40.0%
AAGCTTAATAGCAGCCTTAC+TGG - chr1_2:8205798-8205817 Msa0053690:CDS 40.0%
GAATATTTGCATAGGCAACG+CGG + chr1_2:8204745-8204764 None:intergenic 40.0%
TGAGCTACTGATAGACATTG+AGG - chr1_2:8205076-8205095 Msa0053690:intron 40.0%
CTACTGATAGACATTGAGGT+AGG - chr1_2:8205080-8205099 Msa0053690:intron 40.0%
ATAGACATTGAGGTAGGACT+TGG - chr1_2:8205086-8205105 Msa0053690:intron 40.0%
GATATCTAAGTTCTCCAGCA+GGG + chr1_2:8205112-8205131 None:intergenic 40.0%
AGATATCTAAGTTCTCCAGC+AGG + chr1_2:8205113-8205132 None:intergenic 40.0%
!!! CTTTTTCAGAATGCCTAGAC+TGG - chr1_2:8205135-8205154 Msa0053690:CDS 40.0%
TACCAAGAAATACCCAGTCT+AGG + chr1_2:8205151-8205170 None:intergenic 40.0%
GACTGGGTATTTCTTGGTAT+TGG - chr1_2:8205152-8205171 Msa0053690:CDS 40.0%
GCTTTACTCATGATGCTGAA+AGG - chr1_2:8205311-8205330 Msa0053690:intron 40.0%
CGCTTATGGTGACATCATAA+GGG + chr1_2:8205507-8205526 None:intergenic 40.0%
CCTTATGATGTCACCATAAG+CGG - chr1_2:8205505-8205524 Msa0053690:intron 40.0%
CTTCAAATAGTCAGCCATTC+TGG + chr1_2:8205625-8205644 None:intergenic 40.0%
! AATGGCTGACTATTTGAAGC+AGG - chr1_2:8205626-8205645 Msa0053690:intron 40.0%
! TATTTGAAGCAGGATGGCAA+AGG - chr1_2:8205636-8205655 Msa0053690:intron 40.0%
AAGCTTAATAGCAGCCTTAC+TGG - chr1_2:8205798-8205817 Msa0053690:CDS 40.0%
AAACAGCATCTCGTCTTTCG+CGG - chr1_2:8204486-8204505 Msa0053690:three_prime_UTR 45.0%
TCGCGGTTTCTTGGTAAAAC+AGG - chr1_2:8204503-8204522 Msa0053690:three_prime_UTR 45.0%
CGCGGTTTCTTGGTAAAACA+GGG - chr1_2:8204504-8204523 Msa0053690:three_prime_UTR 45.0%
TGCCTAGACTGGGTATTTCT+TGG - chr1_2:8205146-8205165 Msa0053690:CDS 45.0%
AGCACCCTGAAACAATATGC+AGG + chr1_2:8205266-8205285 None:intergenic 45.0%
! CAGTCTTATAAGCGTGTACG+TGG - chr1_2:8205343-8205362 Msa0053690:intron 45.0%
AAATTGGAAATGCAGGGGAC+AGG - chr1_2:8205462-8205481 Msa0053690:intron 45.0%
TATGGTGACATCATAAGGGC+TGG + chr1_2:8205503-8205522 None:intergenic 45.0%
CCGCTTATGGTGACATCATA+AGG + chr1_2:8205508-8205527 None:intergenic 45.0%
ATATCTGGCCTCACTAATCC+TGG + chr1_2:8205536-8205555 None:intergenic 45.0%
GCCAGATATCATGCATGAGA+GGG - chr1_2:8205547-8205566 Msa0053690:intron 45.0%
TCCCTCTCATGCATGATATC+TGG + chr1_2:8205551-8205570 None:intergenic 45.0%
! TCTGGAATCTCAATCCTCGA+GGG + chr1_2:8205601-8205620 None:intergenic 45.0%
TTGAGATTCCAGAGCCAGAA+TGG - chr1_2:8205608-8205627 Msa0053690:intron 45.0%
! GCTGACTATTTGAAGCAGGA+TGG - chr1_2:8205630-8205649 Msa0053690:intron 45.0%
AAACAGCATCTCGTCTTTCG+CGG - chr1_2:8204486-8204505 Msa0053690:three_prime_UTR 45.0%
TCGCGGTTTCTTGGTAAAAC+AGG - chr1_2:8204503-8204522 Msa0053690:three_prime_UTR 45.0%
CGCGGTTTCTTGGTAAAACA+GGG - chr1_2:8204504-8204523 Msa0053690:three_prime_UTR 45.0%
TGCCTAGACTGGGTATTTCT+TGG - chr1_2:8205146-8205165 Msa0053690:CDS 45.0%
AGCACCCTGAAACAATATGC+AGG + chr1_2:8205266-8205285 None:intergenic 45.0%
! CAGTCTTATAAGCGTGTACG+TGG - chr1_2:8205343-8205362 Msa0053690:intron 45.0%
AAATTGGAAATGCAGGGGAC+AGG - chr1_2:8205462-8205481 Msa0053690:intron 45.0%
TATGGTGACATCATAAGGGC+TGG + chr1_2:8205503-8205522 None:intergenic 45.0%
CCGCTTATGGTGACATCATA+AGG + chr1_2:8205508-8205527 None:intergenic 45.0%
ATATCTGGCCTCACTAATCC+TGG + chr1_2:8205536-8205555 None:intergenic 45.0%
GCCAGATATCATGCATGAGA+GGG - chr1_2:8205547-8205566 Msa0053690:intron 45.0%
TCCCTCTCATGCATGATATC+TGG + chr1_2:8205551-8205570 None:intergenic 45.0%
! TCTGGAATCTCAATCCTCGA+GGG + chr1_2:8205601-8205620 None:intergenic 45.0%
TTGAGATTCCAGAGCCAGAA+TGG - chr1_2:8205608-8205627 Msa0053690:intron 45.0%
! GCTGACTATTTGAAGCAGGA+TGG - chr1_2:8205630-8205649 Msa0053690:intron 45.0%
TCTCGTCTTTCGCGGTTTCT+TGG - chr1_2:8204494-8204513 Msa0053690:three_prime_UTR 50.0%
ACCTCCTCTTCTTCTCCAGA+TGG - chr1_2:8204562-8204581 Msa0053690:three_prime_UTR 50.0%
ACCATCTGGAGAAGAAGAGG+AGG + chr1_2:8204566-8204585 None:intergenic 50.0%
CGAACCATCTGGAGAAGAAG+AGG + chr1_2:8204569-8204588 None:intergenic 50.0%
!! CTTTGGAGTCACGAACCATC+TGG + chr1_2:8204580-8204599 None:intergenic 50.0%
ATAGGCAACGCGGAGTTTGA+AGG + chr1_2:8204735-8204754 None:intergenic 50.0%
CTTATAAGCGTGTACGTGGC+AGG - chr1_2:8205347-8205366 Msa0053690:intron 50.0%
AATCCTGGACTCACCGCTTA+TGG + chr1_2:8205521-8205540 None:intergenic 50.0%
GGCCAGATATCATGCATGAG+AGG - chr1_2:8205546-8205565 Msa0053690:intron 50.0%
ATTTCCTCAGAGATCCCTCG+AGG - chr1_2:8205584-8205603 Msa0053690:intron 50.0%
!! CTCTGGAATCTCAATCCTCG+AGG + chr1_2:8205602-8205621 None:intergenic 50.0%
!! ATAGTCAGCCATTCTGGCTC+TGG + chr1_2:8205619-8205638 None:intergenic 50.0%
TCTCGTCTTTCGCGGTTTCT+TGG - chr1_2:8204494-8204513 Msa0053690:three_prime_UTR 50.0%
ACCTCCTCTTCTTCTCCAGA+TGG - chr1_2:8204562-8204581 Msa0053690:three_prime_UTR 50.0%
ACCATCTGGAGAAGAAGAGG+AGG + chr1_2:8204566-8204585 None:intergenic 50.0%
CGAACCATCTGGAGAAGAAG+AGG + chr1_2:8204569-8204588 None:intergenic 50.0%
!! CTTTGGAGTCACGAACCATC+TGG + chr1_2:8204580-8204599 None:intergenic 50.0%
ATAGGCAACGCGGAGTTTGA+AGG + chr1_2:8204735-8204754 None:intergenic 50.0%
CTTATAAGCGTGTACGTGGC+AGG - chr1_2:8205347-8205366 Msa0053690:intron 50.0%
AATCCTGGACTCACCGCTTA+TGG + chr1_2:8205521-8205540 None:intergenic 50.0%
GGCCAGATATCATGCATGAG+AGG - chr1_2:8205546-8205565 Msa0053690:intron 50.0%
ATTTCCTCAGAGATCCCTCG+AGG - chr1_2:8205584-8205603 Msa0053690:intron 50.0%
!! CTCTGGAATCTCAATCCTCG+AGG + chr1_2:8205602-8205621 None:intergenic 50.0%
!! ATAGTCAGCCATTCTGGCTC+TGG + chr1_2:8205619-8205638 None:intergenic 50.0%
! GCAGGTTGACCGAGGTATGA+TGG - chr1_2:8205365-8205384 Msa0053690:intron 55.0%
! ATAAGGGCTGGTGCACCTAC+TGG + chr1_2:8205491-8205510 None:intergenic 55.0%
TCACCATAAGCGGTGAGTCC+AGG - chr1_2:8205515-8205534 Msa0053690:intron 55.0%
! CGGTGAGTCCAGGATTAGTG+AGG - chr1_2:8205525-8205544 Msa0053690:intron 55.0%
CAATCCTCGAGGGATCTCTG+AGG + chr1_2:8205591-8205610 None:intergenic 55.0%
! GCAGGTTGACCGAGGTATGA+TGG - chr1_2:8205365-8205384 Msa0053690:intron 55.0%
! ATAAGGGCTGGTGCACCTAC+TGG + chr1_2:8205491-8205510 None:intergenic 55.0%
TCACCATAAGCGGTGAGTCC+AGG - chr1_2:8205515-8205534 Msa0053690:intron 55.0%
! CGGTGAGTCCAGGATTAGTG+AGG - chr1_2:8205525-8205544 Msa0053690:intron 55.0%
CAATCCTCGAGGGATCTCTG+AGG + chr1_2:8205591-8205610 None:intergenic 55.0%
! TGTACGTGGCAGGTTGACCG+AGG - chr1_2:8205357-8205376 Msa0053690:intron 60.0%
! TGTACGTGGCAGGTTGACCG+AGG - chr1_2:8205357-8205376 Msa0053690:intron 60.0%
GAGGTAGGACTTGGCCCTGC+TGG - chr1_2:8205095-8205114 Msa0053690:intron 65.0%
GCAGGGGACAGGCTTCCAGT+AGG - chr1_2:8205473-8205492 Msa0053690:intron 65.0%
GAGGTAGGACTTGGCCCTGC+TGG - chr1_2:8205095-8205114 Msa0053690:intron 65.0%
GCAGGGGACAGGCTTCCAGT+AGG - chr1_2:8205473-8205492 Msa0053690:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 8204452 8205863 8204452 ID=Msa0053690;Name=Msa0053690
chr1_2 mRNA 8204452 8205863 8204452 ID=Msa0053690-mRNA-1;Parent=Msa0053690;Name=Msa0053690-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|0.33|0.5|1|1|1|4|299|157
chr1_2 exon 8204452 8204838 8204452 ID=Msa0053690-mRNA-1:exon:2952;Parent=Msa0053690-mRNA-1
chr1_2 exon 8204947 8205035 8204947 ID=Msa0053690-mRNA-1:exon:2951;Parent=Msa0053690-mRNA-1
chr1_2 exon 8205131 8205295 8205131 ID=Msa0053690-mRNA-1:exon:2950;Parent=Msa0053690-mRNA-1
chr1_2 exon 8205732 8205863 8205732 ID=Msa0053690-mRNA-1:exon:2949;Parent=Msa0053690-mRNA-1
chr1_2 CDS 8205732 8205863 8205732 ID=Msa0053690-mRNA-1:cds;Parent=Msa0053690-mRNA-1
chr1_2 CDS 8205131 8205295 8205131 ID=Msa0053690-mRNA-1:cds;Parent=Msa0053690-mRNA-1
chr1_2 CDS 8204947 8205035 8204947 ID=Msa0053690-mRNA-1:cds;Parent=Msa0053690-mRNA-1
chr1_2 CDS 8204751 8204838 8204751 ID=Msa0053690-mRNA-1:cds;Parent=Msa0053690-mRNA-1
chr1_2 three_prime_UTR 8204452 8204750 8204452 ID=Msa0053690-mRNA-1:three_prime_utr;Parent=Msa0053690-mRNA-1
Gene Sequence

>Msa0053690

GTTCCTTTGTTATGTGGATTTCACTCTTTGGAGAAAACAGCATCTCGTCTTTCGCGGTTTCTTGGTAAAACAGGGAAGCATTTATTTTTATCTGATGGTAAGAATGAAAAACCTCCTCTTCTTCTCCAGATGATGTATAGCGACTATATGAAGAGTTATAGAGAGAATATGGCAAATTTCTTAGAATCTGAGCTACTGATAGACATTGAGGTAGGACTTGGCCCTGCTGGAGAACTTAGATATCTTTCTTTTTCAGAATGCCTAGACTGGGTATTTCTTGGTATTGGAGAATTTAATGGTGCTCATGATGCCGAGATTCTTACATTAAGCTTTACTCATGATGCTGAAAGGTGTAGTTATCAGTCTTATAAGCGTGTACGTGGCAGGGGACAGGCTTCCAGTAGGTGCACCAGCCCTTATGATGTCACCATAAGCGGTGAGTCCAGGATTAGTGAGGCCAGATATCATGCATGA
Protein sequence

>Msa0053690

VPLLCGFHSLEKTASRLSRFLGKTGKHLFLSDGKNEKPPLLLQMMYSDYMKSYRENMANFLESELLIDIEVGLGPAGELRYLSFSECLDWVFLGIGEFNGAHDAEILTLSFTHDAERCSYQSYKRVRGRGQASSRCTSPYDVTISGESRISEARYHA*