AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0337500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337500 MtrunA17_Chr2g0323051 65.000 80 26 2 46 125 227 304 4.42e-26 102
Msa0337500 MtrunA17_Chr3g0140681 93.478 46 3 0 1 46 208 253 1.32e-23 94.4
Msa0337500 MtrunA17_Chr4g0023601 56.164 73 29 1 30 99 135 207 1.59e-20 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337500 AT2G32290.1 72.222 54 15 0 46 99 219 272 2.59e-20 86.3
Msa0337500 AT4G15210.2 62.963 54 20 0 46 99 156 209 4.12e-19 82.4
Msa0337500 AT4G15210.1 62.963 54 20 0 46 99 156 209 5.52e-19 82.4
Msa0337500 AT4G15210.3 62.963 54 20 0 46 99 103 156 5.81e-19 82.0
Msa0337500 AT4G15210.4 62.963 54 20 0 46 99 103 156 5.81e-19 82.0
Msa0337500 AT4G00490.1 49.091 55 28 0 46 100 236 290 1.28e-11 61.2

Find 54 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATGTTTATAGATTAATT+AGG 0.166605 2_4:-57546049 Msa0337500:three_prime_UTR
TATGATAATTTAATCAAAAT+TGG 0.267934 2_4:-57545978 Msa0337500:three_prime_UTR
TCTCGTCTTTCGCGGTTTCT+TGG 0.271778 2_4:-57547332 Msa0337500:CDS
GACTGGGTATTTCTTGGTAT+TGG 0.274714 2_4:-57546674 Msa0337500:CDS
ATATGTTTATAGATTAATTA+GGG 0.278241 2_4:-57546048 Msa0337500:three_prime_UTR
CTTCAAATAGTCAGCCATTC+TGG 0.282618 2_4:+57546197 None:intergenic
AAGCTTAATAGCAGCCTTAC+TGG 0.305599 2_4:-57546021 Msa0337500:three_prime_UTR
GAAGAGTTATAGAGAGAATA+TGG 0.309022 2_4:-57546789 Msa0337500:CDS
GTTATGTGGATTTCACTCTT+TGG 0.332758 2_4:-57547366 Msa0337500:CDS
TCGCGGTTTCTTGGTAAAAC+AGG 0.335407 2_4:-57547323 Msa0337500:CDS
TGCCTAGACTGGGTATTTCT+TGG 0.342837 2_4:-57546680 Msa0337500:CDS
AATCCTGGACTCACCGCTTA+TGG 0.358745 2_4:+57546308 None:intergenic
CCGCTTATGGTGACATCATA+AGG 0.359764 2_4:+57546321 None:intergenic
TCCCTCTCATGCATGATATC+TGG 0.374941 2_4:+57546278 None:intergenic
CTTTAGGTTCCTTTGTTATG+TGG 0.393628 2_4:-57547380 Msa0337500:intron
ATAAGGGCTGGTGCACCTAC+TGG 0.427494 2_4:+57546338 None:intergenic
ATAGTCAGCCATTCTGGCTC+TGG 0.443426 2_4:+57546203 None:intergenic
TATATCTATATATATGTTCA+AGG 0.447915 2_4:+57546113 None:intergenic
ATATCTGGCCTCACTAATCC+TGG 0.482839 2_4:+57546293 None:intergenic
AAACAGCATCTCGTCTTTCG+CGG 0.485483 2_4:-57547340 Msa0337500:CDS
GAGGTAGGACTTGGCCCTGC+TGG 0.486945 2_4:-57546731 Msa0337500:CDS
CTTATAAGCGTGTACGTGGC+AGG 0.500665 2_4:-57546479 Msa0337500:CDS
TCACCATAAGCGGTGAGTCC+AGG 0.505916 2_4:-57546311 Msa0337500:three_prime_UTR
CGCTTATGGTGACATCATAA+GGG 0.505983 2_4:+57546322 None:intergenic
CGCGGTTTCTTGGTAAAACA+GGG 0.510516 2_4:-57547322 Msa0337500:CDS
ACCTCCTCTTCTTCTCCAGA+TGG 0.522445 2_4:-57547264 Msa0337500:intron
AAAGCTTAATGTAAGAATCT+CGG 0.527749 2_4:+57546533 None:intergenic
TTGAGATTCCAGAGCCAGAA+TGG 0.530384 2_4:-57546211 Msa0337500:three_prime_UTR
TTGTCAAATATGAACCAGTA+AGG 0.531051 2_4:+57546007 None:intergenic
AGATATCTAAGTTCTCCAGC+AGG 0.532605 2_4:+57546716 None:intergenic
AAATTGGAAATGCAGGGGAC+AGG 0.536511 2_4:-57546364 Msa0337500:intron
TATGGTGACATCATAAGGGC+TGG 0.556208 2_4:+57546326 None:intergenic
AATGGCTGACTATTTGAAGC+AGG 0.562267 2_4:-57546193 Msa0337500:three_prime_UTR
CGAACCATCTGGAGAAGAAG+AGG 0.566975 2_4:+57547260 None:intergenic
ATGTTTATAGATTAATTAGG+GGG 0.570090 2_4:-57546046 Msa0337500:three_prime_UTR
GCTTTACTCATGATGCTGAA+AGG 0.577029 2_4:-57546515 Msa0337500:CDS
CCTTATGATGTCACCATAAG+CGG 0.579231 2_4:-57546321 Msa0337500:three_prime_UTR
TACCAAGAAATACCCAGTCT+AGG 0.594121 2_4:+57546678 None:intergenic
GCCAGATATCATGCATGAGA+GGG 0.594778 2_4:-57546279 Msa0337500:three_prime_UTR
GGCCAGATATCATGCATGAG+AGG 0.594930 2_4:-57546280 Msa0337500:three_prime_UTR
ATAGACATTGAGGTAGGACT+TGG 0.597655 2_4:-57546740 Msa0337500:CDS
TGAGCTACTGATAGACATTG+AGG 0.599509 2_4:-57546750 Msa0337500:CDS
CAGTCTTATAAGCGTGTACG+TGG 0.609552 2_4:-57546483 Msa0337500:CDS
CAGAGATCCTTAAAAGCTCG+AGG 0.621293 2_4:-57546235 Msa0337500:three_prime_UTR
TATTTGAAGCAGGATGGCAA+AGG 0.629381 2_4:-57546183 Msa0337500:three_prime_UTR
GATATCTAAGTTCTCCAGCA+GGG 0.632849 2_4:+57546717 None:intergenic
CTACTGATAGACATTGAGGT+AGG 0.639731 2_4:-57546746 Msa0337500:CDS
GCTGACTATTTGAAGCAGGA+TGG 0.643683 2_4:-57546189 Msa0337500:three_prime_UTR
ACCATCTGGAGAAGAAGAGG+AGG 0.646740 2_4:+57547263 None:intergenic
TATGTTTATAGATTAATTAG+GGG 0.649576 2_4:-57546047 Msa0337500:three_prime_UTR
CGGTGAGTCCAGGATTAGTG+AGG 0.671978 2_4:-57546301 Msa0337500:three_prime_UTR
GCAGGGGACAGGCTTCCAGT+AGG 0.671994 2_4:-57546353 Msa0337500:intron
GAGTGAAATCCACATAACAA+AGG 0.672556 2_4:+57547371 None:intergenic
TGTACGTGGCAGGTTGACCG+AGG 0.702164 2_4:-57546469 Msa0337500:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATGTTTATAGATTAATT+AGG - chr2_4:57547425-57547444 Msa0337500:intron 10.0%
!! ATATGTTTATAGATTAATTA+GGG - chr2_4:57547426-57547445 Msa0337500:intron 10.0%
!! TATGATAATTTAATCAAAAT+TGG - chr2_4:57547496-57547515 Msa0337500:intron 10.0%
!! TATATGTTTATAGATTAATT+AGG - chr2_4:57547425-57547444 Msa0337500:intron 10.0%
!! ATATGTTTATAGATTAATTA+GGG - chr2_4:57547426-57547445 Msa0337500:intron 10.0%
!! TTACATGAATTTAATATCTT+TGG + chr2_4:57546245-57546264 None:intergenic 15.0%
!! AAAGATATTAAATTCATGTA+AGG - chr2_4:57546244-57546263 Msa0337500:three_prime_UTR 15.0%
!! AAAGAAGTAATAATAAAAAG+TGG + chr2_4:57546560-57546579 None:intergenic 15.0%
!!! CATAACAAATTTTAGTAAAA+TGG + chr2_4:57547061-57547080 None:intergenic 15.0%
!! TGGAATATTTATATGAAAAT+TGG - chr2_4:57547094-57547113 Msa0337500:intron 15.0%
!! TATATCTATATATATGTTCA+AGG + chr2_4:57547364-57547383 None:intergenic 15.0%
!! TATGTTTATAGATTAATTAG+GGG - chr2_4:57547427-57547446 Msa0337500:intron 15.0%
!! TTACATGAATTTAATATCTT+TGG + chr2_4:57546245-57546264 None:intergenic 15.0%
!! AAAGATATTAAATTCATGTA+AGG - chr2_4:57546244-57546263 Msa0337500:three_prime_UTR 15.0%
!! AAAGAAGTAATAATAAAAAG+TGG + chr2_4:57546560-57546579 None:intergenic 15.0%
!!! CATAACAAATTTTAGTAAAA+TGG + chr2_4:57547061-57547080 None:intergenic 15.0%
!! TGGAATATTTATATGAAAAT+TGG - chr2_4:57547094-57547113 Msa0337500:intron 15.0%
!! TATATCTATATATATGTTCA+AGG + chr2_4:57547364-57547383 None:intergenic 15.0%
!! TATGTTTATAGATTAATTAG+GGG - chr2_4:57547427-57547446 Msa0337500:intron 15.0%
!!! AAGCATTTATTTTTATCTGA+TGG - chr2_4:57546175-57546194 Msa0337500:three_prime_UTR 20.0%
!! TATTATATCTCTTTCTCTAT+AGG - chr2_4:57546344-57546363 Msa0337500:exon 20.0%
!! TGAACTAAAACAAAAGAATA+CGG + chr2_4:57546423-57546442 None:intergenic 20.0%
!! TGTTATGTAATGTTTAATGT+TGG - chr2_4:57547074-57547093 Msa0337500:intron 20.0%
!! ATGTTTATAGATTAATTAGG+GGG - chr2_4:57547428-57547447 Msa0337500:intron 20.0%
!!! AAGCATTTATTTTTATCTGA+TGG - chr2_4:57546175-57546194 Msa0337500:three_prime_UTR 20.0%
!! TATTATATCTCTTTCTCTAT+AGG - chr2_4:57546344-57546363 Msa0337500:exon 20.0%
!! TGAACTAAAACAAAAGAATA+CGG + chr2_4:57546423-57546442 None:intergenic 20.0%
!! TGTTATGTAATGTTTAATGT+TGG - chr2_4:57547074-57547093 Msa0337500:intron 20.0%
!! ATGTTTATAGATTAATTAGG+GGG - chr2_4:57547428-57547447 Msa0337500:intron 20.0%
! GTTATTGAGGGTAATTAAAT+AGG - chr2_4:57545981-57546000 Msa0337500:three_prime_UTR 25.0%
!! TTTGGAATTTCACATACTTT+AGG - chr2_4:57546078-57546097 Msa0337500:three_prime_UTR 25.0%
!! TATGTTGTTTGTTCATGATT+TGG + chr2_4:57546319-57546338 None:intergenic 25.0%
! GATAGTAATAGAATATGCAA+AGG + chr2_4:57546456-57546475 None:intergenic 25.0%
!!! TTCAAGTTTTGCTTATGTTT+AGG + chr2_4:57546524-57546543 None:intergenic 25.0%
! AAAGCTTAATGTAAGAATCT+CGG + chr2_4:57546944-57546963 None:intergenic 25.0%
! TATATGAAAATTGGAAATGC+AGG - chr2_4:57547103-57547122 Msa0337500:intron 25.0%
! ATATGAAAATTGGAAATGCA+GGG - chr2_4:57547104-57547123 Msa0337500:intron 25.0%
! GTTATTGAGGGTAATTAAAT+AGG - chr2_4:57545981-57546000 Msa0337500:three_prime_UTR 25.0%
!! TTTGGAATTTCACATACTTT+AGG - chr2_4:57546078-57546097 Msa0337500:three_prime_UTR 25.0%
!! TATGTTGTTTGTTCATGATT+TGG + chr2_4:57546319-57546338 None:intergenic 25.0%
! GATAGTAATAGAATATGCAA+AGG + chr2_4:57546456-57546475 None:intergenic 25.0%
!!! TTCAAGTTTTGCTTATGTTT+AGG + chr2_4:57546524-57546543 None:intergenic 25.0%
! AAAGCTTAATGTAAGAATCT+CGG + chr2_4:57546944-57546963 None:intergenic 25.0%
! TATATGAAAATTGGAAATGC+AGG - chr2_4:57547103-57547122 Msa0337500:intron 25.0%
! ATATGAAAATTGGAAATGCA+GGG - chr2_4:57547104-57547123 Msa0337500:intron 25.0%
! GATTTGGTTTCTAAACCATA+AGG + chr2_4:57546303-57546322 None:intergenic 30.0%
TCATTTAAACTTACAGACAG+TGG + chr2_4:57546490-57546509 None:intergenic 30.0%
GAAGAGTTATAGAGAGAATA+TGG - chr2_4:57546685-57546704 Msa0337500:CDS 30.0%
TGAACATATATGTATACACG+TGG + chr2_4:57546878-57546897 None:intergenic 30.0%
CGTGTATACATATATGTTCA+TGG - chr2_4:57546878-57546897 Msa0337500:intron 30.0%
TATGAAAATTGGAAATGCAG+GGG - chr2_4:57547105-57547124 Msa0337500:intron 30.0%
!! CTCTGAGGAAATTTTGTATT+TGG + chr2_4:57547224-57547243 None:intergenic 30.0%
TGAAGAAACTGATGAAAAAG+AGG - chr2_4:57547329-57547348 Msa0337500:CDS 30.0%
GAAGAAACTGATGAAAAAGA+GGG - chr2_4:57547330-57547349 Msa0337500:CDS 30.0%
TTGTCAAATATGAACCAGTA+AGG + chr2_4:57547470-57547489 None:intergenic 30.0%
! GATTTGGTTTCTAAACCATA+AGG + chr2_4:57546303-57546322 None:intergenic 30.0%
TCATTTAAACTTACAGACAG+TGG + chr2_4:57546490-57546509 None:intergenic 30.0%
GAAGAGTTATAGAGAGAATA+TGG - chr2_4:57546685-57546704 Msa0337500:CDS 30.0%
TGAACATATATGTATACACG+TGG + chr2_4:57546878-57546897 None:intergenic 30.0%
CGTGTATACATATATGTTCA+TGG - chr2_4:57546878-57546897 Msa0337500:intron 30.0%
TATGAAAATTGGAAATGCAG+GGG - chr2_4:57547105-57547124 Msa0337500:intron 30.0%
!! CTCTGAGGAAATTTTGTATT+TGG + chr2_4:57547224-57547243 None:intergenic 30.0%
TGAAGAAACTGATGAAAAAG+AGG - chr2_4:57547329-57547348 Msa0337500:CDS 30.0%
GAAGAAACTGATGAAAAAGA+GGG - chr2_4:57547330-57547349 Msa0337500:CDS 30.0%
TTGTCAAATATGAACCAGTA+AGG + chr2_4:57547470-57547489 None:intergenic 30.0%
CATGTCATACTAATCTGTGT+TGG - chr2_4:57546035-57546054 Msa0337500:three_prime_UTR 35.0%
! AAAGCTTCATGACATGGTTT+TGG - chr2_4:57546060-57546079 Msa0337500:three_prime_UTR 35.0%
! CTTTAGGTTCCTTTGTTATG+TGG - chr2_4:57546094-57546113 Msa0337500:three_prime_UTR 35.0%
GAGTGAAATCCACATAACAA+AGG + chr2_4:57546106-57546125 None:intergenic 35.0%
GTTATGTGGATTTCACTCTT+TGG - chr2_4:57546108-57546127 Msa0337500:three_prime_UTR 35.0%
! TGATTTTCGCAACTTCCTTA+TGG - chr2_4:57546285-57546304 Msa0337500:three_prime_UTR 35.0%
TTCTCTATAGGTCTGCTTTA+CGG - chr2_4:57546356-57546375 Msa0337500:intron 35.0%
GTCGTAACGAATATTTGCAT+AGG + chr2_4:57546401-57546420 None:intergenic 35.0%
TTTCCCTTAGTTCCCTTAAT+TGG - chr2_4:57546633-57546652 Msa0337500:intron 35.0%
ACACCAATTAAGGGAACTAA+GGG + chr2_4:57546639-57546658 None:intergenic 35.0%
AACACCAATTAAGGGAACTA+AGG + chr2_4:57546640-57546659 None:intergenic 35.0%
CTGCATCAAACACCAATTAA+GGG + chr2_4:57546648-57546667 None:intergenic 35.0%
TCTGCATCAAACACCAATTA+AGG + chr2_4:57546649-57546668 None:intergenic 35.0%
!! TTTTTCAGAATGCCTAGACT+GGG - chr2_4:57546784-57546803 Msa0337500:CDS 35.0%
TGTATCCTGCATATTGTTTC+AGG - chr2_4:57546906-57546925 Msa0337500:intron 35.0%
GTATCCTGCATATTGTTTCA+GGG - chr2_4:57546907-57546926 Msa0337500:intron 35.0%
GAAATTGTTCCATCATACCT+CGG + chr2_4:57547025-57547044 None:intergenic 35.0%
CATGTCATACTAATCTGTGT+TGG - chr2_4:57546035-57546054 Msa0337500:three_prime_UTR 35.0%
! AAAGCTTCATGACATGGTTT+TGG - chr2_4:57546060-57546079 Msa0337500:three_prime_UTR 35.0%
! CTTTAGGTTCCTTTGTTATG+TGG - chr2_4:57546094-57546113 Msa0337500:three_prime_UTR 35.0%
GAGTGAAATCCACATAACAA+AGG + chr2_4:57546106-57546125 None:intergenic 35.0%
GTTATGTGGATTTCACTCTT+TGG - chr2_4:57546108-57546127 Msa0337500:three_prime_UTR 35.0%
! TGATTTTCGCAACTTCCTTA+TGG - chr2_4:57546285-57546304 Msa0337500:three_prime_UTR 35.0%
TTCTCTATAGGTCTGCTTTA+CGG - chr2_4:57546356-57546375 Msa0337500:intron 35.0%
GTCGTAACGAATATTTGCAT+AGG + chr2_4:57546401-57546420 None:intergenic 35.0%
TTTCCCTTAGTTCCCTTAAT+TGG - chr2_4:57546633-57546652 Msa0337500:intron 35.0%
ACACCAATTAAGGGAACTAA+GGG + chr2_4:57546639-57546658 None:intergenic 35.0%
AACACCAATTAAGGGAACTA+AGG + chr2_4:57546640-57546659 None:intergenic 35.0%
CTGCATCAAACACCAATTAA+GGG + chr2_4:57546648-57546667 None:intergenic 35.0%
TCTGCATCAAACACCAATTA+AGG + chr2_4:57546649-57546668 None:intergenic 35.0%
!! TTTTTCAGAATGCCTAGACT+GGG - chr2_4:57546784-57546803 Msa0337500:CDS 35.0%
TGTATCCTGCATATTGTTTC+AGG - chr2_4:57546906-57546925 Msa0337500:intron 35.0%
GTATCCTGCATATTGTTTCA+GGG - chr2_4:57546907-57546926 Msa0337500:intron 35.0%
GAAATTGTTCCATCATACCT+CGG + chr2_4:57547025-57547044 None:intergenic 35.0%
TTGGTGAAAGCTTCATGACA+TGG - chr2_4:57546054-57546073 Msa0337500:three_prime_UTR 40.0%
GAATATTTGCATAGGCAACG+CGG + chr2_4:57546393-57546412 None:intergenic 40.0%
TGAGCTACTGATAGACATTG+AGG - chr2_4:57546724-57546743 Msa0337500:CDS 40.0%
CTACTGATAGACATTGAGGT+AGG - chr2_4:57546728-57546747 Msa0337500:CDS 40.0%
ATAGACATTGAGGTAGGACT+TGG - chr2_4:57546734-57546753 Msa0337500:CDS 40.0%
GATATCTAAGTTCTCCAGCA+GGG + chr2_4:57546760-57546779 None:intergenic 40.0%
AGATATCTAAGTTCTCCAGC+AGG + chr2_4:57546761-57546780 None:intergenic 40.0%
!!! CTTTTTCAGAATGCCTAGAC+TGG - chr2_4:57546783-57546802 Msa0337500:CDS 40.0%
TACCAAGAAATACCCAGTCT+AGG + chr2_4:57546799-57546818 None:intergenic 40.0%
GACTGGGTATTTCTTGGTAT+TGG - chr2_4:57546800-57546819 Msa0337500:CDS 40.0%
GCTTTACTCATGATGCTGAA+AGG - chr2_4:57546959-57546978 Msa0337500:intron 40.0%
CGCTTATGGTGACATCATAA+GGG + chr2_4:57547155-57547174 None:intergenic 40.0%
CCTTATGATGTCACCATAAG+CGG - chr2_4:57547153-57547172 Msa0337500:intron 40.0%
! TCTCAATCCTCGAGCTTTTA+AGG + chr2_4:57547249-57547268 None:intergenic 40.0%
CTTCAAATAGTCAGCCATTC+TGG + chr2_4:57547280-57547299 None:intergenic 40.0%
! AATGGCTGACTATTTGAAGC+AGG - chr2_4:57547281-57547300 Msa0337500:CDS 40.0%
! TATTTGAAGCAGGATGGCAA+AGG - chr2_4:57547291-57547310 Msa0337500:CDS 40.0%
AAGCTTAATAGCAGCCTTAC+TGG - chr2_4:57547453-57547472 Msa0337500:intron 40.0%
TTGGTGAAAGCTTCATGACA+TGG - chr2_4:57546054-57546073 Msa0337500:three_prime_UTR 40.0%
GAATATTTGCATAGGCAACG+CGG + chr2_4:57546393-57546412 None:intergenic 40.0%
TGAGCTACTGATAGACATTG+AGG - chr2_4:57546724-57546743 Msa0337500:CDS 40.0%
CTACTGATAGACATTGAGGT+AGG - chr2_4:57546728-57546747 Msa0337500:CDS 40.0%
ATAGACATTGAGGTAGGACT+TGG - chr2_4:57546734-57546753 Msa0337500:CDS 40.0%
GATATCTAAGTTCTCCAGCA+GGG + chr2_4:57546760-57546779 None:intergenic 40.0%
AGATATCTAAGTTCTCCAGC+AGG + chr2_4:57546761-57546780 None:intergenic 40.0%
!!! CTTTTTCAGAATGCCTAGAC+TGG - chr2_4:57546783-57546802 Msa0337500:CDS 40.0%
TACCAAGAAATACCCAGTCT+AGG + chr2_4:57546799-57546818 None:intergenic 40.0%
GACTGGGTATTTCTTGGTAT+TGG - chr2_4:57546800-57546819 Msa0337500:CDS 40.0%
GCTTTACTCATGATGCTGAA+AGG - chr2_4:57546959-57546978 Msa0337500:intron 40.0%
CGCTTATGGTGACATCATAA+GGG + chr2_4:57547155-57547174 None:intergenic 40.0%
CCTTATGATGTCACCATAAG+CGG - chr2_4:57547153-57547172 Msa0337500:intron 40.0%
! TCTCAATCCTCGAGCTTTTA+AGG + chr2_4:57547249-57547268 None:intergenic 40.0%
CTTCAAATAGTCAGCCATTC+TGG + chr2_4:57547280-57547299 None:intergenic 40.0%
! AATGGCTGACTATTTGAAGC+AGG - chr2_4:57547281-57547300 Msa0337500:CDS 40.0%
! TATTTGAAGCAGGATGGCAA+AGG - chr2_4:57547291-57547310 Msa0337500:CDS 40.0%
AAGCTTAATAGCAGCCTTAC+TGG - chr2_4:57547453-57547472 Msa0337500:intron 40.0%
AAACAGCATCTCGTCTTTCG+CGG - chr2_4:57546134-57546153 Msa0337500:three_prime_UTR 45.0%
TCGCGGTTTCTTGGTAAAAC+AGG - chr2_4:57546151-57546170 Msa0337500:three_prime_UTR 45.0%
CGCGGTTTCTTGGTAAAACA+GGG - chr2_4:57546152-57546171 Msa0337500:three_prime_UTR 45.0%
TGCCTAGACTGGGTATTTCT+TGG - chr2_4:57546794-57546813 Msa0337500:CDS 45.0%
AGCACCCTGAAACAATATGC+AGG + chr2_4:57546914-57546933 None:intergenic 45.0%
! CAGTCTTATAAGCGTGTACG+TGG - chr2_4:57546991-57547010 Msa0337500:intron 45.0%
AAATTGGAAATGCAGGGGAC+AGG - chr2_4:57547110-57547129 Msa0337500:intron 45.0%
TATGGTGACATCATAAGGGC+TGG + chr2_4:57547151-57547170 None:intergenic 45.0%
CCGCTTATGGTGACATCATA+AGG + chr2_4:57547156-57547175 None:intergenic 45.0%
ATATCTGGCCTCACTAATCC+TGG + chr2_4:57547184-57547203 None:intergenic 45.0%
GCCAGATATCATGCATGAGA+GGG - chr2_4:57547195-57547214 Msa0337500:intron 45.0%
TCCCTCTCATGCATGATATC+TGG + chr2_4:57547199-57547218 None:intergenic 45.0%
!! CGAGCTTTTAAGGATCTCTG+AGG + chr2_4:57547239-57547258 None:intergenic 45.0%
CAGAGATCCTTAAAAGCTCG+AGG - chr2_4:57547239-57547258 Msa0337500:intron 45.0%
TTGAGATTCCAGAGCCAGAA+TGG - chr2_4:57547263-57547282 Msa0337500:intron 45.0%
! GCTGACTATTTGAAGCAGGA+TGG - chr2_4:57547285-57547304 Msa0337500:CDS 45.0%
AAACAGCATCTCGTCTTTCG+CGG - chr2_4:57546134-57546153 Msa0337500:three_prime_UTR 45.0%
TCGCGGTTTCTTGGTAAAAC+AGG - chr2_4:57546151-57546170 Msa0337500:three_prime_UTR 45.0%
CGCGGTTTCTTGGTAAAACA+GGG - chr2_4:57546152-57546171 Msa0337500:three_prime_UTR 45.0%
TGCCTAGACTGGGTATTTCT+TGG - chr2_4:57546794-57546813 Msa0337500:CDS 45.0%
AGCACCCTGAAACAATATGC+AGG + chr2_4:57546914-57546933 None:intergenic 45.0%
! CAGTCTTATAAGCGTGTACG+TGG - chr2_4:57546991-57547010 Msa0337500:intron 45.0%
AAATTGGAAATGCAGGGGAC+AGG - chr2_4:57547110-57547129 Msa0337500:intron 45.0%
TATGGTGACATCATAAGGGC+TGG + chr2_4:57547151-57547170 None:intergenic 45.0%
CCGCTTATGGTGACATCATA+AGG + chr2_4:57547156-57547175 None:intergenic 45.0%
ATATCTGGCCTCACTAATCC+TGG + chr2_4:57547184-57547203 None:intergenic 45.0%
GCCAGATATCATGCATGAGA+GGG - chr2_4:57547195-57547214 Msa0337500:intron 45.0%
TCCCTCTCATGCATGATATC+TGG + chr2_4:57547199-57547218 None:intergenic 45.0%
!! CGAGCTTTTAAGGATCTCTG+AGG + chr2_4:57547239-57547258 None:intergenic 45.0%
CAGAGATCCTTAAAAGCTCG+AGG - chr2_4:57547239-57547258 Msa0337500:intron 45.0%
TTGAGATTCCAGAGCCAGAA+TGG - chr2_4:57547263-57547282 Msa0337500:intron 45.0%
! GCTGACTATTTGAAGCAGGA+TGG - chr2_4:57547285-57547304 Msa0337500:CDS 45.0%
TCTCGTCTTTCGCGGTTTCT+TGG - chr2_4:57546142-57546161 Msa0337500:three_prime_UTR 50.0%
ACCTCCTCTTCTTCTCCAGA+TGG - chr2_4:57546210-57546229 Msa0337500:three_prime_UTR 50.0%
ACCATCTGGAGAAGAAGAGG+AGG + chr2_4:57546214-57546233 None:intergenic 50.0%
CGAACCATCTGGAGAAGAAG+AGG + chr2_4:57546217-57546236 None:intergenic 50.0%
!! CTTTGGAGTCACGAACCATC+TGG + chr2_4:57546228-57546247 None:intergenic 50.0%
ATAGGCAACGCGGAGTTTGA+AGG + chr2_4:57546383-57546402 None:intergenic 50.0%
CTTATAAGCGTGTACGTGGC+AGG - chr2_4:57546995-57547014 Msa0337500:intron 50.0%
AATCCTGGACTCACCGCTTA+TGG + chr2_4:57547169-57547188 None:intergenic 50.0%
GGCCAGATATCATGCATGAG+AGG - chr2_4:57547194-57547213 Msa0337500:intron 50.0%
!! ATAGTCAGCCATTCTGGCTC+TGG + chr2_4:57547274-57547293 None:intergenic 50.0%
TCTCGTCTTTCGCGGTTTCT+TGG - chr2_4:57546142-57546161 Msa0337500:three_prime_UTR 50.0%
ACCTCCTCTTCTTCTCCAGA+TGG - chr2_4:57546210-57546229 Msa0337500:three_prime_UTR 50.0%
ACCATCTGGAGAAGAAGAGG+AGG + chr2_4:57546214-57546233 None:intergenic 50.0%
CGAACCATCTGGAGAAGAAG+AGG + chr2_4:57546217-57546236 None:intergenic 50.0%
!! CTTTGGAGTCACGAACCATC+TGG + chr2_4:57546228-57546247 None:intergenic 50.0%
ATAGGCAACGCGGAGTTTGA+AGG + chr2_4:57546383-57546402 None:intergenic 50.0%
CTTATAAGCGTGTACGTGGC+AGG - chr2_4:57546995-57547014 Msa0337500:intron 50.0%
AATCCTGGACTCACCGCTTA+TGG + chr2_4:57547169-57547188 None:intergenic 50.0%
GGCCAGATATCATGCATGAG+AGG - chr2_4:57547194-57547213 Msa0337500:intron 50.0%
!! ATAGTCAGCCATTCTGGCTC+TGG + chr2_4:57547274-57547293 None:intergenic 50.0%
! GCAGGTTGACCGAGGTATGA+TGG - chr2_4:57547013-57547032 Msa0337500:intron 55.0%
! ATAAGGGCTGGTGCACCTAC+TGG + chr2_4:57547139-57547158 None:intergenic 55.0%
TCACCATAAGCGGTGAGTCC+AGG - chr2_4:57547163-57547182 Msa0337500:intron 55.0%
! CGGTGAGTCCAGGATTAGTG+AGG - chr2_4:57547173-57547192 Msa0337500:intron 55.0%
! GCAGGTTGACCGAGGTATGA+TGG - chr2_4:57547013-57547032 Msa0337500:intron 55.0%
! ATAAGGGCTGGTGCACCTAC+TGG + chr2_4:57547139-57547158 None:intergenic 55.0%
TCACCATAAGCGGTGAGTCC+AGG - chr2_4:57547163-57547182 Msa0337500:intron 55.0%
! CGGTGAGTCCAGGATTAGTG+AGG - chr2_4:57547173-57547192 Msa0337500:intron 55.0%
! TGTACGTGGCAGGTTGACCG+AGG - chr2_4:57547005-57547024 Msa0337500:intron 60.0%
! TGTACGTGGCAGGTTGACCG+AGG - chr2_4:57547005-57547024 Msa0337500:intron 60.0%
GAGGTAGGACTTGGCCCTGC+TGG - chr2_4:57546743-57546762 Msa0337500:CDS 65.0%
GCAGGGGACAGGCTTCCAGT+AGG - chr2_4:57547121-57547140 Msa0337500:intron 65.0%
GAGGTAGGACTTGGCCCTGC+TGG - chr2_4:57546743-57546762 Msa0337500:CDS 65.0%
GCAGGGGACAGGCTTCCAGT+AGG - chr2_4:57547121-57547140 Msa0337500:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 57545978 57547518 57545978 ID=Msa0337500;Name=Msa0337500
chr2_4 mRNA 57545978 57547518 57545978 ID=Msa0337500-mRNA-1;Parent=Msa0337500;Name=Msa0337500-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|0.5|0.6|0.8|0.5|0.8|5|376|138
chr2_4 exon 57545978 57546371 57545978 ID=Msa0337500-mRNA-1:exon:17562;Parent=Msa0337500-mRNA-1
chr2_4 exon 57546470 57546568 57546470 ID=Msa0337500-mRNA-1:exon:17561;Parent=Msa0337500-mRNA-1
chr2_4 exon 57546664 57546828 57546664 ID=Msa0337500-mRNA-1:exon:17560;Parent=Msa0337500-mRNA-1
chr2_4 exon 57547265 57547396 57547265 ID=Msa0337500-mRNA-1:exon:17559;Parent=Msa0337500-mRNA-1
chr2_4 exon 57547516 57547518 57547516 ID=Msa0337500-mRNA-1:exon:17558;Parent=Msa0337500-mRNA-1
chr2_4 CDS 57547516 57547518 57547516 ID=Msa0337500-mRNA-1:cds;Parent=Msa0337500-mRNA-1
chr2_4 CDS 57547265 57547396 57547265 ID=Msa0337500-mRNA-1:cds;Parent=Msa0337500-mRNA-1
chr2_4 CDS 57546664 57546828 57546664 ID=Msa0337500-mRNA-1:cds;Parent=Msa0337500-mRNA-1
chr2_4 CDS 57546470 57546568 57546470 ID=Msa0337500-mRNA-1:cds;Parent=Msa0337500-mRNA-1
chr2_4 CDS 57546354 57546371 57546354 ID=Msa0337500-mRNA-1:cds;Parent=Msa0337500-mRNA-1
chr2_4 three_prime_UTR 57545978 57546353 57545978 ID=Msa0337500-mRNA-1:three_prime_utr;Parent=Msa0337500-mRNA-1
Gene Sequence

>Msa0337500

ATGGTTCCTTTGTTATGTGGATTTCACTCTTTGGAGAAAACAGCATCTCGTCTTTCGCGGTTTCTTGGTAAAACAGGGAAGCATTTATTTTTATCTGATGGTAAGAATGAAAAACCTCCTCTTCTTCTCCAGATGATGTATAGCGACTATATGAAGAGTTATAGAGAGAATATGGCAAATTTCTTAGAATCTGAGCTACTGATAGACATTGAGGTAGGACTTGGCCCTGCTGGAGAACTTAGATATCTTTCTTTTTCAGAATGCCTAGACTGGGTATTTCTTGGTATTGGAGAATTTAATGGTGCTCATGATGCCGAGATTCTTACATTAAGCTTTACTCATGATGCTGAAAGGTGTAGTTATCAGTCTTATAAGCGTGTACGTGGCAGGTTGACCGAGGGGACAGGCTTCCAGTAG
Protein sequence

>Msa0337500

MVPLLCGFHSLEKTASRLSRFLGKTGKHLFLSDGKNEKPPLLLQMMYSDYMKSYRENMANFLESELLIDIEVGLGPAGELRYLSFSECLDWVFLGIGEFNGAHDAEILTLSFTHDAERCSYQSYKRVRGRLTEGTGFQ*