AlfalfaGEDB | Msa1253400

M. sativa cultivar zhongmu-4 / Msa1253400

KEGG: -
GO: -
PFAM: PF03514.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253400	XP_003597373.1	54.601	163	43	3	20	176	284	421	6.06e-40	161

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253400	sp\|O65015\|AMYB_TRIRP	77.778	54	12	0	239	292	154	207	8.90e-21	96.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253400	G7IRV1	54.601	163	43	3	20	176	284	421	2.90e-40	161

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TFs/TRs:

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Gene ID	Type	Classification
Msa1253400	TF	GRAS

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006880	Msa1253400	0.809877	1.523084e-50	-8.615850e-47
Msa0036370	Msa1253400	0.803517	3.357466e-49	-8.615850e-47
Msa0049390	Msa1253400	0.805010	1.640644e-49	-8.615850e-47
Msa0052630	Msa1253400	0.803790	2.946022e-49	-8.615850e-47
Msa0053690	Msa1253400	0.864779	9.507724e-65	-8.615850e-47
Msa0053700	Msa1253400	0.885245	9.604400e-72	-8.615850e-47
Msa0055150	Msa1253400	0.800072	1.711092e-48	-8.615850e-47
Msa0067890	Msa1253400	0.806196	9.250929e-50	-8.615850e-47
Msa0068020	Msa1253400	0.801121	1.045462e-48	-8.615850e-47
Msa0074750	Msa1253400	0.807021	6.194540e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253400	MtrunA17_Chr2g0328391	54.601	163	43	3	20	176	212	349	5.61e-44	160
Msa1253400	MtrunA17_Chr2g0323051	53.982	113	42	4	215	327	211	313	6.30e-27	111
Msa1253400	MtrunA17_Chr4g0031711	63.889	72	26	0	105	176	340	411	1.18e-25	108
Msa1253400	MtrunA17_Chr4g0031721	42.748	131	58	1	95	208	285	415	8.09e-24	102
Msa1253400	MtrunA17_Chr4g0031701	62.857	70	26	0	107	176	235	304	4.78e-23	100
Msa1253400	MtrunA17_Chr3g0140681	76.667	60	8	1	186	239	194	253	9.69e-23	98.2
Msa1253400	MtrunA17_Chr4g0031731	61.429	70	27	0	107	176	235	304	2.40e-22	98.2
Msa1253400	MtrunA17_Chr7g0238891	46.903	113	52	2	108	212	354	466	2.85e-22	98.2
Msa1253400	MtrunA17_Chr4g0023601	58.904	73	27	1	223	292	135	207	2.54e-20	92.0
Msa1253400	MtrunA17_Chr2g0328381	53.165	79	37	0	97	175	246	324	3.19e-19	89.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253400	AT2G32290.1	52.222	90	41	2	239	328	219	306	4.63e-21	94.7
Msa1253400	AT1G07530.1	51.765	85	39	1	93	175	361	445	3.42e-20	92.4
Msa1253400	AT4G15210.3	66.667	54	18	0	239	292	103	156	1.92e-19	89.4
Msa1253400	AT4G15210.4	66.667	54	18	0	239	292	103	156	1.92e-19	89.4
Msa1253400	AT4G15210.2	66.667	54	18	0	239	292	156	209	1.99e-19	89.4
Msa1253400	AT4G15210.1	66.667	54	18	0	239	292	156	209	2.22e-19	89.4
Msa1253400	AT1G07520.2	55.814	86	25	3	108	186	139	218	1.37e-18	87.4
Msa1253400	AT1G07520.4	55.814	86	25	3	108	186	139	218	1.37e-18	87.4
Msa1253400	AT1G07520.1	61.765	68	23	1	108	175	303	367	1.82e-18	87.0
Msa1253400	AT1G07520.3	61.765	68	23	1	108	175	304	368	1.84e-18	87.0

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Find 105 sgRNAs with CRISPR-Local

Find 462 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGAACGAGAGAAGGG+AGG	0.577096	8_2:-50855503	Msa1253400:CDS
AAAAGAAGGAAAACAGAACA+CGG	0.558132	8_2:-50855547	Msa1253400:CDS
AAACAGAACGAGAGAAGGGA+GGG	0.502519	8_2:-50855502	Msa1253400:CDS
AAACAGCATCTCGTCTTTCG+CGG	0.485483	8_2:-50849900	Msa1253400:CDS
AAAGAAGGAAAACAGAACAC+GGG	0.584884	8_2:-50855546	Msa1253400:CDS
AAAGCTTAATGTAAGAATCT+CGG	0.527749	8_2:+50849093	None:intergenic
AAATTACCTTGTAAGTATGG+TGG	0.624420	8_2:+50854805	None:intergenic
AAATTGGAAATGCAGGGGAC+AGG	0.536511	8_2:-50848924	Msa1253400:intron
AAGAAGCCGATGGAGGAAGA+AGG	0.492336	8_2:-50855463	Msa1253400:CDS
AAGCTTAATAGCAACCTTAC+TGG	0.376747	8_2:-50848581	Msa1253400:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGAAGGAAAACAGAACA+CGG	-	chr8_2:50848711-50848730	Msa1253400:three_prime_UTR	30.0%
AAAAAAAACAGAACGAGAGA+AGG	-	chr8_2:50848751-50848770	Msa1253400:three_prime_UTR	30.0%
AAAAAAACAGAACGAGAGAA+GGG	-	chr8_2:50848752-50848771	Msa1253400:three_prime_UTR	30.0%
TCAAATTTCAACATGCATGA+GGG	+	chr8_2:50848875-50848894	None:intergenic	30.0%
ATCAAATTTCAACATGCATG+AGG	+	chr8_2:50848876-50848895	None:intergenic	30.0%
CTTGGTATAAATTGCACATA+AGG	-	chr8_2:50849147-50849166	Msa1253400:intron	30.0%
TACCATTAATACCTCTTCTA+TGG	+	chr8_2:50849424-50849443	None:intergenic	30.0%
AAATTACCTTGTAAGTATGG+TGG	+	chr8_2:50849456-50849475	None:intergenic	30.0%
ATGTATGATAGCAGTAATGT+TGG	-	chr8_2:50850231-50850250	Msa1253400:intron	30.0%
CAGTTATTAACTATTGCTCT+AGG	+	chr8_2:50850579-50850598	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	50848538	50855742	50848538	ID=Msa1253400;Name=Msa1253400
chr8_2	mRNA	50848538	50855742	50848538	ID=Msa1253400-mRNA-1;Parent=Msa1253400;Name=Msa1253400-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0\|0.25\|0.22\|0.77\|0.75\|0.88\|9\|306\|351
chr8_2	exon	50848538	50848931	50848538	ID=Msa1253400-mRNA-1:exon:15551;Parent=Msa1253400-mRNA-1
chr8_2	exon	50849040	50849128	50849040	ID=Msa1253400-mRNA-1:exon:15550;Parent=Msa1253400-mRNA-1
chr8_2	exon	50849224	50849388	50849224	ID=Msa1253400-mRNA-1:exon:15549;Parent=Msa1253400-mRNA-1
chr8_2	exon	50849825	50849956	50849825	ID=Msa1253400-mRNA-1:exon:15548;Parent=Msa1253400-mRNA-1
chr8_2	exon	50851112	50851168	50851112	ID=Msa1253400-mRNA-1:exon:15547;Parent=Msa1253400-mRNA-1
chr8_2	exon	50851640	50851884	50851640	ID=Msa1253400-mRNA-1:exon:15546;Parent=Msa1253400-mRNA-1
chr8_2	exon	50854812	50854929	50854812	ID=Msa1253400-mRNA-1:exon:15545;Parent=Msa1253400-mRNA-1
chr8_2	exon	50855429	50855582	50855429	ID=Msa1253400-mRNA-1:exon:15544;Parent=Msa1253400-mRNA-1
chr8_2	exon	50855735	50855742	50855735	ID=Msa1253400-mRNA-1:exon:15543;Parent=Msa1253400-mRNA-1
chr8_2	CDS	50855735	50855742	50855735	ID=Msa1253400-mRNA-1:cds;Parent=Msa1253400-mRNA-1
chr8_2	CDS	50855429	50855582	50855429	ID=Msa1253400-mRNA-1:cds;Parent=Msa1253400-mRNA-1
chr8_2	CDS	50854812	50854929	50854812	ID=Msa1253400-mRNA-1:cds;Parent=Msa1253400-mRNA-1
chr8_2	CDS	50851640	50851884	50851640	ID=Msa1253400-mRNA-1:cds;Parent=Msa1253400-mRNA-1
chr8_2	CDS	50851112	50851168	50851112	ID=Msa1253400-mRNA-1:cds;Parent=Msa1253400-mRNA-1
chr8_2	CDS	50849825	50849956	50849825	ID=Msa1253400-mRNA-1:cds;Parent=Msa1253400-mRNA-1
chr8_2	CDS	50849224	50849388	50849224	ID=Msa1253400-mRNA-1:cds;Parent=Msa1253400-mRNA-1
chr8_2	CDS	50849040	50849128	50849040	ID=Msa1253400-mRNA-1:cds;Parent=Msa1253400-mRNA-1
chr8_2	CDS	50848844	50848931	50848844	ID=Msa1253400-mRNA-1:cds;Parent=Msa1253400-mRNA-1
chr8_2	three_prime_UTR	50848538	50848843	50848538	ID=Msa1253400-mRNA-1:three_prime_utr;Parent=Msa1253400-mRNA-1

Gene Sequence

>Msa1253400

ATGACCAGAAAAGGAAAAAAAAAAAGAAGGAAAACAGAACACGGGACAGAGAAGAAAAAAGAAAAAAAACAGAACGAGAGAAGGGAGGGAGAGGAGAAGAAGGAAAAGAAGCCGATGGAGGAAGAAGGGAAATTGGAGAAGAAGGCAAGCTTCAATTTTAAGGTTAATGAACTTGCTACATGCCATGGTGAAATCAAGGTCGTAAGGGCAATTGAAGCTAAGAAAGATAAGGCCATAGAAGAGGTATTAATGGTAATTACTAAGCCACCATACTTACAAGTGGTGGAGAGTAGAACACAGGTTGGTGAACTTGATGGAGGGAAGGCACGTTCGAAGAAACAAGGGAGGAAGAGGGATGTTGTGGATTTGAGGACCCTTTTGGTTCTGTGTGCACAAGCTGTGTCTGCTAATGACAATAGGACTGCTAATGAATTGTTGAAGCAGAATAGGAAGCATTCTTGTCCTTCAGGTGATGCATCACAGAGAATGGCTCATTACTTTGCGAATGCGATTGAAGCGCGCATGATTCAAGTCAACAGAAGTGACTATGACAATGTAATTGGAGCTTTGCCATATCTGAAAGTTCCTTTGTTATGTGGATTTCACTCTTTGGAGAAAACAGCATCTCGTCTTTCGCGGTTTCTTGGTAAAACAGGGAAGCATTTATTTTTATCTGATGGTAAGAATGAAAAACCTCCTCTTCTTCTCCAGATGATGTATAGCGACTATATGAAGAGTTATAGAGAGAATATGGCAAATTTCTTAGAATCTGAGCTACTGATAGACATTGAGGTAGGACTTGGCCCTGCTGGAGAACTTAGATATCTTTCTTTTTCAGAATGCCTAGGCTGGGTATTTCCTGGTATTGGAGAATTTAATGGTGCTCATGATGCCGAGATTCTTACATTAAGCTTTACTCATGATGCTGAAAGGTGTAGTTATCAGTCTTATAAGCGTGTACGTGGCAGGGGACAGGCTTCCAGTAGGTGCACCAGCCCTTATGATGTCACCATAAGCGGTGAGTCCAGGATTAGTGAGGCCAGATATCATGCATGA

Protein sequence

>Msa1253400

MTRKGKKKRRKTEHGTEKKKEKKQNERREGEEKKEKKPMEEEGKLEKKASFNFKVNELATCHGEIKVVRAIEAKKDKAIEEVLMVITKPPYLQVVESRTQVGELDGGKARSKKQGRKRDVVDLRTLLVLCAQAVSANDNRTANELLKQNRKHSCPSGDASQRMAHYFANAIEARMIQVNRSDYDNVIGALPYLKVPLLCGFHSLEKTASRLSRFLGKTGKHLFLSDGKNEKPPLLLQMMYSDYMKSYRENMANFLESELLIDIEVGLGPAGELRYLSFSECLGWVFPGIGEFNGAHDAEILTLSFTHDAERCSYQSYKRVRGRGQASSRCTSPYDVTISGESRISEARYHA*