AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0809960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 82 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATGTTTAAGCATAAT+TGG 0.227612 5_3:-37814616 Msa0809960:intron
AGATTTGGCCATTGCAGATT+TGG 0.265527 5_3:+37816276 None:intergenic
CATGAACAGTGTTGCGATTT+GGG 0.277970 5_3:+37816169 None:intergenic
TATTTGGATTATGTATTTAG+AGG 0.331200 5_3:+37816354 None:intergenic
AGTAAGGATGAGGCAAAATA+TGG 0.338880 5_3:-37814648 Msa0809960:five_prime_UTR
CCATGAACAGTGTTGCGATT+TGG 0.352695 5_3:+37816168 None:intergenic
CCAAATCGCAACACTGTTCA+TGG 0.355738 5_3:-37816168 Msa0809960:five_prime_UTR
GAACGAGTGATGTTTAGATT+TGG 0.367908 5_3:+37816261 None:intergenic
ATTGGAAAAGGGGACTTTCC+CGG 0.373983 5_3:+37816383 None:intergenic
GAACACATGAAAGATGTATC+AGG 0.374816 5_3:-37814199 Msa0809960:intron
TTCTGATGATACAGATCAAT+GGG 0.377166 5_3:+37814765 None:intergenic
GGAAATAATGCCCTGGGAAA+AGG 0.405380 5_3:-37814253 Msa0809960:five_prime_UTR
TTCCGATGATACACCTCAAT+GGG 0.406158 5_3:+37813664 None:intergenic
CGGTGAAGAACAAAGGGATT+TGG 0.407573 5_3:+37816219 None:intergenic
GAAGCATAAAAGGGATGAAA+AGG 0.423775 5_3:-37814680 Msa0809960:five_prime_UTR
TGAAGTTGAAGGTGGATGAT+AGG 0.433777 5_3:+37814796 None:intergenic
TTTCGTGTAACTTCTTTCAC+GGG 0.436604 5_3:-37813847 Msa0809960:CDS
ATTCAACTTGTGAGAGTGAA+TGG 0.442397 5_3:-37814227 Msa0809960:five_prime_UTR
AGAACCCGATGAACAGTGTT+GGG 0.454102 5_3:+37816110 None:intergenic
CTTCTGATGATACAGATCAA+TGG 0.456816 5_3:+37814764 None:intergenic
TGAGGCAAAATATGGAAGAA+TGG 0.460558 5_3:-37814640 Msa0809960:five_prime_UTR
AGTGTTGGGATTTAAGATAC+AGG 0.461647 5_3:+37816124 None:intergenic
GAAAAGGATGTGAACAAGTA+AGG 0.463154 5_3:-37814664 Msa0809960:five_prime_UTR
GAAGAGGCAAAGGTAGAGGA+AGG 0.464396 5_3:-37814823 Msa0809960:five_prime_UTR
GAGGCAAAATATGGAAGAAT+GGG 0.469998 5_3:-37814639 Msa0809960:five_prime_UTR
GGAGCTAAATGATCTGTAGA+AGG 0.474490 5_3:-37815404 Msa0809960:five_prime_UTR
AAATCCCAACACTGTTCATC+GGG 0.475409 5_3:-37816114 Msa0809960:five_prime_UTR
ATCGGAAGAAACTAAAATTG+AGG 0.487071 5_3:-37813648 Msa0809960:CDS
TCGGAAGAAACTAAAATTGA+GGG 0.497291 5_3:-37813647 Msa0809960:CDS
GGATTTGGTTCAGTTTCAAT+CGG 0.501068 5_3:+37816234 None:intergenic
GAGTGAGGCTGAGTTGTTTG+CGG 0.503889 5_3:-37813879 Msa0809960:CDS
TGAGAACGGTGAAGAACAAA+GGG 0.506076 5_3:+37816213 None:intergenic
CTTCCGATGATACACCTCAA+TGG 0.508352 5_3:+37813663 None:intergenic
AAAGGAAGAGGCAAAGGTAG+AGG 0.514762 5_3:-37814827 Msa0809960:five_prime_UTR
GACAGTAAAGGAAGAGGCAA+AGG 0.516865 5_3:-37814833 Msa0809960:intron
AGAGGAAGACGCAAAGGTAG+AGG 0.521904 5_3:-37813727 Msa0809960:CDS
TTCACCTTCCATCCCCATTG+AGG 0.526879 5_3:-37813677 Msa0809960:CDS
CCTGTACTGCATGATGTTGA+AGG 0.533167 5_3:+37815379 None:intergenic
TAAATCCCAACACTGTTCAT+CGG 0.533942 5_3:-37816115 Msa0809960:five_prime_UTR
AGACAGTGGAAATAATGCCC+TGG 0.534611 5_3:-37814260 Msa0809960:five_prime_UTR
CCTTCAACATCATGCAGTAC+AGG 0.540407 5_3:-37815379 Msa0809960:intron
GGCTGAGAGCGGAGGGAGTG+AGG 0.541825 5_3:-37813894 Msa0809960:CDS
GAACGAGAGATCTCGAATGA+GGG 0.551036 5_3:+37816298 None:intergenic
GGAACGAGAGATCTCGAATG+AGG 0.557478 5_3:+37816297 None:intergenic
GACAGTGGAAATAATGCCCT+GGG 0.560030 5_3:-37814259 Msa0809960:five_prime_UTR
GACAGTAGAGGAAGACGCAA+AGG 0.562445 5_3:-37813733 Msa0809960:intron
GAAGACGCAAAGGTAGAGGA+AGG 0.563013 5_3:-37813723 Msa0809960:CDS
GGGATGGAAGGTGAAGTTGA+AGG 0.563546 5_3:+37813685 None:intergenic
GGGGTGGAAGGTGAAGTTGA+AGG 0.563546 5_3:+37814785 None:intergenic
CTCTCGTTCCAAATCTGCAA+TGG 0.563736 5_3:-37816284 Msa0809960:five_prime_UTR
TGAAGTTGAAGGTGGATGAA+AGG 0.563768 5_3:+37813696 None:intergenic
TAGAACCCGATGAACAGTGT+TGG 0.565549 5_3:+37816109 None:intergenic
CTGGTTTGAAGAGGTTGAAG+AGG 0.567087 5_3:-37814316 Msa0809960:intron
CTTAACAGTGTACGTTGGAA+CGG 0.569539 5_3:-37813915 Msa0809960:intron
GTGTTGGGATTTAAGATACA+GGG 0.571925 5_3:+37816125 None:intergenic
ATACAGATCAATGGGGTGGA+AGG 0.572935 5_3:+37814773 None:intergenic
TTGAGAACGGTGAAGAACAA+AGG 0.573710 5_3:+37816212 None:intergenic
ATGATACACCTCAATGGGGA+TGG 0.573795 5_3:+37813669 None:intergenic
ATTGATGACAGTAAAGGAAG+AGG 0.580307 5_3:-37814839 Msa0809960:intron
ATGGAAGGTGAAGTTGAAGG+TGG 0.584449 5_3:+37813688 None:intergenic
TTGGAACGGCTGAGAGCGGA+GGG 0.588560 5_3:-37813901 Msa0809960:CDS
TACACCTCAATGGGGATGGA+AGG 0.596472 5_3:+37813673 None:intergenic
GATGTGAACAAGTAAGGATG+AGG 0.596783 5_3:-37814658 Msa0809960:five_prime_UTR
GTGGAAGGTGAAGTTGAAGG+TGG 0.597288 5_3:+37814788 None:intergenic
TACGTTGGAACGGCTGAGAG+CGG 0.602342 5_3:-37813905 Msa0809960:CDS
ACTGAAAGCAAAGAAGATGA+AGG 0.603713 5_3:-37814292 Msa0809960:five_prime_UTR
TCCCCATTGAGGTGTATCAT+CGG 0.605021 5_3:-37813666 Msa0809960:CDS
AAGAGGCAAAGGTAGAGGAA+GGG 0.606199 5_3:-37814822 Msa0809960:five_prime_UTR
GTTGGAACGGCTGAGAGCGG+AGG 0.606352 5_3:-37813902 Msa0809960:CDS
TTTATCTTAACAGTGTACGT+TGG 0.612159 5_3:-37813920 Msa0809960:intron
CAAATCGCAACACTGTTCAT+GGG 0.617397 5_3:-37816167 Msa0809960:five_prime_UTR
GGCTTCATAGTGCATCGTGA+AGG 0.627058 5_3:-37815425 Msa0809960:intron
AACGAGAGATCTCGAATGAG+GGG 0.633603 5_3:+37816299 None:intergenic
GATGATACAGATCAATGGGG+TGG 0.638378 5_3:+37814769 None:intergenic
CTGTACTGCATGATGTTGAA+GGG 0.646034 5_3:+37815380 None:intergenic
TTCGTGTAACTTCTTTCACG+GGG 0.646991 5_3:-37813846 Msa0809960:CDS
AAGAACAAGAGATTTGAGAA+CGG 0.648564 5_3:+37816199 None:intergenic
GAAGGCACAAAGTAAGACAG+TGG 0.655331 5_3:-37814274 Msa0809960:five_prime_UTR
AAGACGCAAAGGTAGAGGAA+GGG 0.660530 5_3:-37813722 Msa0809960:CDS
TCGTGTAACTTCTTTCACGG+GGG 0.732087 5_3:-37813845 Msa0809960:CDS
TCCGATGATACACCTCAATG+GGG 0.735633 5_3:+37813665 None:intergenic
TCTGATGATACAGATCAATG+GGG 0.765833 5_3:+37814766 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAAATGTTTTTTTTTATT+TGG + chr5_3:37813701-37813720 None:intergenic 10.0%
!!! TAAAAGTGTTTTTATTTTTA+TGG - chr5_3:37814241-37814260 Msa0809960:five_prime_UTR 10.0%
!!! TTTTATTATTAAAGTTTGAA+GGG - chr5_3:37814758-37814777 Msa0809960:five_prime_UTR 10.0%
!!! TGTTTTTACTATAATATGTT+AGG - chr5_3:37814134-37814153 Msa0809960:intron 15.0%
!!! AATACAATATAACTTGATTT+TGG - chr5_3:37814461-37814480 Msa0809960:intron 15.0%
!! TATAACATGTTCAATTATTA+CGG - chr5_3:37814590-37814609 Msa0809960:intron 15.0%
!!! CTTTTATTATTAAAGTTTGA+AGG - chr5_3:37814757-37814776 Msa0809960:five_prime_UTR 15.0%
!! TGAATCAATTTATTGTATTA+AGG - chr5_3:37814974-37814993 Msa0809960:intron 15.0%
!! TCTAAAGGTAAAAATATAAT+TGG + chr5_3:37815014-37815033 None:intergenic 15.0%
!! AAGTAAAATAATATTACAGA+AGG + chr5_3:37815138-37815157 None:intergenic 15.0%
!!! GAAAATATTGTTGTTAAATA+CGG + chr5_3:37815681-37815700 None:intergenic 15.0%
!!! TAAAAATTATTTGTGTTTTC+TGG - chr5_3:37815701-37815720 Msa0809960:intron 15.0%
!! TATTTGGATTATGTATTTAG+AGG + chr5_3:37813685-37813704 None:intergenic 20.0%
!!! CAGAAGAATAATGAAATTTT+GGG + chr5_3:37813982-37814001 None:intergenic 20.0%
!!! ACAGAAGAATAATGAAATTT+TGG + chr5_3:37813983-37814002 None:intergenic 20.0%
!! AAAATGATAACAAGTGAAAA+AGG + chr5_3:37814045-37814064 None:intergenic 20.0%
!! TTGGAATTTCAATTTATCTA+GGG - chr5_3:37814182-37814201 Msa0809960:intron 20.0%
!!! GCATAAAAAAAGCATAAAAT+TGG + chr5_3:37814857-37814876 None:intergenic 20.0%
!!! ATTTTATGCTTTTTTTATGC+CGG - chr5_3:37814857-37814876 Msa0809960:intron 20.0%
!!! CTATGTTATTTAGTTTAGAA+AGG - chr5_3:37814898-37814917 Msa0809960:intron 20.0%
!!! TTTAGCTTTTAGAATAAAGT+TGG - chr5_3:37814928-37814947 Msa0809960:intron 20.0%
!! AGGTAAAAATATAATTGGTT+TGG + chr5_3:37815009-37815028 None:intergenic 20.0%
!! AAGAAAAAATGAAGCATAAA+AGG - chr5_3:37815346-37815365 Msa0809960:intron 20.0%
!! AGAAAAAATGAAGCATAAAA+GGG - chr5_3:37815347-37815366 Msa0809960:intron 20.0%
!! ATCAAATGTTTAAGCATAAT+TGG - chr5_3:37815420-37815439 Msa0809960:intron 20.0%
!!! TCATTTTTGGTTTTTTGTTT+TGG - chr5_3:37815885-37815904 Msa0809960:intron 20.0%
!!! TGTCAACATGATTAATTTTA+TGG - chr5_3:37816004-37816023 Msa0809960:intron 20.0%
!!! ATAACTAGTACTATTTTTTC+TGG - chr5_3:37816059-37816078 Msa0809960:intron 20.0%
!!! ATTTTTTCTGGAAATAGTTA+AGG - chr5_3:37816071-37816090 Msa0809960:intron 20.0%
!! ATAAACTTACATTATAGCAT+CGG + chr5_3:37816101-37816120 None:intergenic 20.0%
! TGTATTTAGAGGTGAAAAAT+TGG + chr5_3:37813674-37813693 None:intergenic 25.0%
! TTTCTCAAGTTCAATCAAAT+AGG - chr5_3:37814072-37814091 Msa0809960:intron 25.0%
! TTCTCAAGTTCAATCAAATA+GGG - chr5_3:37814073-37814092 Msa0809960:intron 25.0%
!!! GAATAAGAACTACTTTTACT+TGG - chr5_3:37814163-37814182 Msa0809960:intron 25.0%
! CTTGGAATTTCAATTTATCT+AGG - chr5_3:37814181-37814200 Msa0809960:intron 25.0%
!! ATAAAGATTTCGTAGGTTTT+GGG - chr5_3:37814274-37814293 Msa0809960:five_prime_UTR 25.0%
! TTTCATAAGTCATCAAAGAT+AGG - chr5_3:37814411-37814430 Msa0809960:intron 25.0%
!! TTGGTTGTTGATTATTGATT+AGG - chr5_3:37814799-37814818 Msa0809960:five_prime_UTR 25.0%
!! TGGTTGTTGATTATTGATTA+GGG - chr5_3:37814800-37814819 Msa0809960:five_prime_UTR 25.0%
! GTCAAAATATTAACAATGAC+CGG + chr5_3:37814879-37814898 None:intergenic 25.0%
! AAAGTTGGATACTTGATTTA+AGG - chr5_3:37814943-37814962 Msa0809960:intron 25.0%
!! TTACATATTGATGACAGTAA+AGG - chr5_3:37815191-37815210 Msa0809960:intron 25.0%
!!! TGGTAGTTTCTTTATTTTGA+AGG - chr5_3:37815440-37815459 Msa0809960:intron 25.0%
!!! GGTAGTTTCTTTATTTTGAA+GGG - chr5_3:37815441-37815460 Msa0809960:intron 25.0%
! GATCAATTTCTACTTAACAA+CGG - chr5_3:37815543-37815562 Msa0809960:intron 25.0%
! TATATATAGCTATCAAATCC+TGG + chr5_3:37815961-37815980 None:intergenic 25.0%
AGAGGTGAAAAATTGGAAAA+GGG + chr5_3:37813667-37813686 None:intergenic 30.0%
TAGAGGTGAAAAATTGGAAA+AGG + chr5_3:37813668-37813687 None:intergenic 30.0%
AAGAACAAGAGATTTGAGAA+CGG + chr5_3:37813840-37813859 None:intergenic 30.0%
ACCTACGAAATCTTTATGAA+AGG + chr5_3:37814271-37814290 None:intergenic 30.0%
! CATAAAGATTTCGTAGGTTT+TGG - chr5_3:37814273-37814292 Msa0809960:five_prime_UTR 30.0%
AAAGTTGTGAGAATGTTGTT+TGG - chr5_3:37814335-37814354 Msa0809960:intron 30.0%
AGCTTATGAACATGTTCATA+AGG - chr5_3:37814522-37814541 Msa0809960:intron 30.0%
CACAACACAGATAAGAAAAA+GGG + chr5_3:37814717-37814736 None:intergenic 30.0%
ACACAACACAGATAAGAAAA+AGG + chr5_3:37814718-37814737 None:intergenic 30.0%
TCAACAACCAATATAAGAGT+GGG + chr5_3:37814790-37814809 None:intergenic 30.0%
ATCAACAACCAATATAAGAG+TGG + chr5_3:37814791-37814810 None:intergenic 30.0%
CACTATACTGAAAACTATCA+CGG + chr5_3:37815065-37815084 None:intergenic 30.0%
TTCAGTATAGTGTATTTCAC+AGG - chr5_3:37815073-37815092 Msa0809960:intron 30.0%
!!! ACAGGTTATGTTTTTGAGTA+GGG - chr5_3:37815091-37815110 Msa0809960:intron 30.0%
TTCTGATGATACAGATCAAT+GGG + chr5_3:37815274-37815293 None:intergenic 30.0%
TTGTACTAAATAGTGCATCA+TGG - chr5_3:37815606-37815625 Msa0809960:intron 30.0%
!!! ACATCTTCATCTGTCATTTT+TGG - chr5_3:37815872-37815891 Msa0809960:intron 30.0%
TGTTTCTCTCACATTATTCA+TGG + chr5_3:37815923-37815942 None:intergenic 30.0%
TTTATCTTAACAGTGTACGT+TGG - chr5_3:37816116-37816135 Msa0809960:five_prime_UTR 30.0%
! TTTTCGTGTAACTTCTTTCA+CGG - chr5_3:37816188-37816207 Msa0809960:five_prime_UTR 30.0%
! TTACTTATTGATGACAGTAG+AGG - chr5_3:37816291-37816310 Msa0809960:five_prime_UTR 30.0%
ATCGGAAGAAACTAAAATTG+AGG - chr5_3:37816388-37816407 Msa0809960:five_prime_UTR 30.0%
TCGGAAGAAACTAAAATTGA+GGG - chr5_3:37816389-37816408 Msa0809960:five_prime_UTR 30.0%
GAGGTGAAAAATTGGAAAAG+GGG + chr5_3:37813666-37813685 None:intergenic 35.0%
GAACGAGTGATGTTTAGATT+TGG + chr5_3:37813778-37813797 None:intergenic 35.0%
! GGATTTGGTTCAGTTTCAAT+CGG + chr5_3:37813805-37813824 None:intergenic 35.0%
! GTGTTGGGATTTAAGATACA+GGG + chr5_3:37813914-37813933 None:intergenic 35.0%
! AGTGTTGGGATTTAAGATAC+AGG + chr5_3:37813915-37813934 None:intergenic 35.0%
TAAATCCCAACACTGTTCAT+CGG - chr5_3:37813921-37813940 Msa0809960:intron 35.0%
!!! TCTTCTGTTTTTACGTTCTG+TGG - chr5_3:37813995-37814014 Msa0809960:intron 35.0%
GCCTTTCATAAAGATTTCGT+AGG - chr5_3:37814267-37814286 Msa0809960:five_prime_UTR 35.0%
GTAACAACCCACTCTTATAT+TGG - chr5_3:37814780-37814799 Msa0809960:five_prime_UTR 35.0%
GCATGGTGCAATAAATCTAA+AGG + chr5_3:37815029-37815048 None:intergenic 35.0%
AGATTTATTGCACCATGCTT+TGG - chr5_3:37815031-37815050 Msa0809960:intron 35.0%
! ACGGATTTAAAACCAAAGCA+TGG + chr5_3:37815046-37815065 None:intergenic 35.0%
!!! CACAGGTTATGTTTTTGAGT+AGG - chr5_3:37815090-37815109 Msa0809960:intron 35.0%
!!! CAGGTTATGTTTTTGAGTAG+GGG - chr5_3:37815092-37815111 Msa0809960:intron 35.0%
! ATTGATGACAGTAAAGGAAG+AGG - chr5_3:37815197-37815216 Msa0809960:intron 35.0%
TCTGATGATACAGATCAATG+GGG + chr5_3:37815273-37815292 None:intergenic 35.0%
CTTCTGATGATACAGATCAA+TGG + chr5_3:37815275-37815294 None:intergenic 35.0%
GAAGCATAAAAGGGATGAAA+AGG - chr5_3:37815356-37815375 Msa0809960:intron 35.0%
GAAAAGGATGTGAACAAGTA+AGG - chr5_3:37815372-37815391 Msa0809960:intron 35.0%
AGTAAGGATGAGGCAAAATA+TGG - chr5_3:37815388-37815407 Msa0809960:five_prime_UTR 35.0%
TGAGGCAAAATATGGAAGAA+TGG - chr5_3:37815396-37815415 Msa0809960:five_prime_UTR 35.0%
GAGGCAAAATATGGAAGAAT+GGG - chr5_3:37815397-37815416 Msa0809960:five_prime_UTR 35.0%
!!! TTGTGTTTTCTGGTTTGAAG+AGG - chr5_3:37815711-37815730 Msa0809960:intron 35.0%
ACTGAAAGCAAAGAAGATGA+AGG - chr5_3:37815744-37815763 Msa0809960:intron 35.0%
! GTTGAATTTTCCTTTTCCCA+GGG + chr5_3:37815796-37815815 None:intergenic 35.0%
! AGTTGAATTTTCCTTTTCCC+AGG + chr5_3:37815797-37815816 None:intergenic 35.0%
ATTCAACTTGTGAGAGTGAA+TGG - chr5_3:37815809-37815828 Msa0809960:intron 35.0%
GAACACATGAAAGATGTATC+AGG - chr5_3:37815837-37815856 Msa0809960:intron 35.0%
CATGAAAGATGTATCAGGTT+TGG - chr5_3:37815842-37815861 Msa0809960:intron 35.0%
TTTCGTGTAACTTCTTTCAC+GGG - chr5_3:37816189-37816208 Msa0809960:five_prime_UTR 35.0%
AGATTTGGCCATTGCAGATT+TGG + chr5_3:37813763-37813782 None:intergenic 40.0%
TGAGAACGGTGAAGAACAAA+GGG + chr5_3:37813826-37813845 None:intergenic 40.0%
TTGAGAACGGTGAAGAACAA+AGG + chr5_3:37813827-37813846 None:intergenic 40.0%
CATGAACAGTGTTGCGATTT+GGG + chr5_3:37813870-37813889 None:intergenic 40.0%
CAAATCGCAACACTGTTCAT+GGG - chr5_3:37813869-37813888 Msa0809960:CDS 40.0%
AAATCCCAACACTGTTCATC+GGG - chr5_3:37813922-37813941 Msa0809960:intron 40.0%
GGAGCTAAATGATCTGTAGA+AGG - chr5_3:37814632-37814651 Msa0809960:five_prime_UTR 40.0%
CTGTACTGCATGATGTTGAA+GGG + chr5_3:37814659-37814678 None:intergenic 40.0%
TGAAGTTGAAGGTGGATGAT+AGG + chr5_3:37815243-37815262 None:intergenic 40.0%
GATGTGAACAAGTAAGGATG+AGG - chr5_3:37815378-37815397 Msa0809960:intron 40.0%
CAATGTGCGATTGACAACAT+TGG + chr5_3:37815507-37815526 None:intergenic 40.0%
GCAGCAGAGTTCAAACAAAA+TGG + chr5_3:37815639-37815658 None:intergenic 40.0%
ACATAAACTAGTATCACGCC+AGG - chr5_3:37815940-37815959 Msa0809960:intron 40.0%
CTTAACAGTGTACGTTGGAA+CGG - chr5_3:37816121-37816140 Msa0809960:five_prime_UTR 40.0%
TTCGTGTAACTTCTTTCACG+GGG - chr5_3:37816190-37816209 Msa0809960:five_prime_UTR 40.0%
! TTTTTCCTGCAGAATTCTGC+AGG - chr5_3:37816224-37816243 Msa0809960:five_prime_UTR 40.0%
AAAAACCTGCAGAATTCTGC+AGG + chr5_3:37816232-37816251 None:intergenic 40.0%
TGAAGTTGAAGGTGGATGAA+AGG + chr5_3:37816343-37816362 None:intergenic 40.0%
TTCCGATGATACACCTCAAT+GGG + chr5_3:37816375-37816394 None:intergenic 40.0%
ATTGGAAAAGGGGACTTTCC+CGG + chr5_3:37813656-37813675 None:intergenic 45.0%
AACGAGAGATCTCGAATGAG+GGG + chr5_3:37813740-37813759 None:intergenic 45.0%
GAACGAGAGATCTCGAATGA+GGG + chr5_3:37813741-37813760 None:intergenic 45.0%
CTCTCGTTCCAAATCTGCAA+TGG - chr5_3:37813752-37813771 Msa0809960:intron 45.0%
CGGTGAAGAACAAAGGGATT+TGG + chr5_3:37813820-37813839 None:intergenic 45.0%
CCATGAACAGTGTTGCGATT+TGG + chr5_3:37813871-37813890 None:intergenic 45.0%
CCAAATCGCAACACTGTTCA+TGG - chr5_3:37813868-37813887 Msa0809960:CDS 45.0%
AGAACCCGATGAACAGTGTT+GGG + chr5_3:37813929-37813948 None:intergenic 45.0%
TAGAACCCGATGAACAGTGT+TGG + chr5_3:37813930-37813949 None:intergenic 45.0%
!!! TCGTAGGTTTTGGGAGTGTT+TGG - chr5_3:37814283-37814302 Msa0809960:five_prime_UTR 45.0%
CCTGTACTGCATGATGTTGA+AGG + chr5_3:37814660-37814679 None:intergenic 45.0%
CCTTCAACATCATGCAGTAC+AGG - chr5_3:37814657-37814676 Msa0809960:five_prime_UTR 45.0%
GACAGTAAAGGAAGAGGCAA+AGG - chr5_3:37815203-37815222 Msa0809960:intron 45.0%
AAAGGAAGAGGCAAAGGTAG+AGG - chr5_3:37815209-37815228 Msa0809960:intron 45.0%
AAGAGGCAAAGGTAGAGGAA+GGG - chr5_3:37815214-37815233 Msa0809960:intron 45.0%
ATACAGATCAATGGGGTGGA+AGG + chr5_3:37815266-37815285 None:intergenic 45.0%
GATGATACAGATCAATGGGG+TGG + chr5_3:37815270-37815289 None:intergenic 45.0%
! CTGGTTTGAAGAGGTTGAAG+AGG - chr5_3:37815720-37815739 Msa0809960:intron 45.0%
! GAAGGCACAAAGTAAGACAG+TGG - chr5_3:37815762-37815781 Msa0809960:intron 45.0%
AGACAGTGGAAATAATGCCC+TGG - chr5_3:37815776-37815795 Msa0809960:intron 45.0%
GACAGTGGAAATAATGCCCT+GGG - chr5_3:37815777-37815796 Msa0809960:intron 45.0%
GGAAATAATGCCCTGGGAAA+AGG - chr5_3:37815783-37815802 Msa0809960:intron 45.0%
TCGTGTAACTTCTTTCACGG+GGG - chr5_3:37816191-37816210 Msa0809960:five_prime_UTR 45.0%
AAGACGCAAAGGTAGAGGAA+GGG - chr5_3:37816314-37816333 Msa0809960:five_prime_UTR 45.0%
ATGGAAGGTGAAGTTGAAGG+TGG + chr5_3:37816351-37816370 None:intergenic 45.0%
ATGATACACCTCAATGGGGA+TGG + chr5_3:37816370-37816389 None:intergenic 45.0%
TCCCCATTGAGGTGTATCAT+CGG - chr5_3:37816370-37816389 Msa0809960:five_prime_UTR 45.0%
TCCGATGATACACCTCAATG+GGG + chr5_3:37816374-37816393 None:intergenic 45.0%
CTTCCGATGATACACCTCAA+TGG + chr5_3:37816376-37816395 None:intergenic 45.0%
GGAACGAGAGATCTCGAATG+AGG + chr5_3:37813742-37813761 None:intergenic 50.0%
GGCTTCATAGTGCATCGTGA+AGG - chr5_3:37814611-37814630 Msa0809960:intron 50.0%
GAAGAGGCAAAGGTAGAGGA+AGG - chr5_3:37815213-37815232 Msa0809960:intron 50.0%
GTGGAAGGTGAAGTTGAAGG+TGG + chr5_3:37815251-37815270 None:intergenic 50.0%
GAGTGAGGCTGAGTTGTTTG+CGG - chr5_3:37816157-37816176 Msa0809960:five_prime_UTR 50.0%
GACAGTAGAGGAAGACGCAA+AGG - chr5_3:37816303-37816322 Msa0809960:five_prime_UTR 50.0%
AGAGGAAGACGCAAAGGTAG+AGG - chr5_3:37816309-37816328 Msa0809960:five_prime_UTR 50.0%
GAAGACGCAAAGGTAGAGGA+AGG - chr5_3:37816313-37816332 Msa0809960:five_prime_UTR 50.0%
GGGATGGAAGGTGAAGTTGA+AGG + chr5_3:37816354-37816373 None:intergenic 50.0%
TTCACCTTCCATCCCCATTG+AGG - chr5_3:37816359-37816378 Msa0809960:five_prime_UTR 50.0%
TACACCTCAATGGGGATGGA+AGG + chr5_3:37816366-37816385 None:intergenic 50.0%
GGGGTGGAAGGTGAAGTTGA+AGG + chr5_3:37815254-37815273 None:intergenic 55.0%
TACGTTGGAACGGCTGAGAG+CGG - chr5_3:37816131-37816150 Msa0809960:five_prime_UTR 55.0%
TTGGAACGGCTGAGAGCGGA+GGG - chr5_3:37816135-37816154 Msa0809960:five_prime_UTR 60.0%
GTTGGAACGGCTGAGAGCGG+AGG - chr5_3:37816134-37816153 Msa0809960:five_prime_UTR 65.0%
GGCTGAGAGCGGAGGGAGTG+AGG - chr5_3:37816142-37816161 Msa0809960:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 37813642 37816416 37813642 ID=Msa0809960;Name=Msa0809960
chr5_3 mRNA 37813642 37816416 37813642 ID=Msa0809960-mRNA-1;Parent=Msa0809960;Name=Msa0809960-mRNA-1;_AED=0.24;_eAED=0.24;_QI=737|0.8|0.66|1|0.4|0.33|6|0|69
chr5_3 exon 37813642 37813750 37813642 ID=Msa0809960-mRNA-1:exon:11598;Parent=Msa0809960-mRNA-1
chr5_3 exon 37813838 37813929 37813838 ID=Msa0809960-mRNA-1:exon:11597;Parent=Msa0809960-mRNA-1
chr5_3 exon 37814200 37814327 37814200 ID=Msa0809960-mRNA-1:exon:11596;Parent=Msa0809960-mRNA-1
chr5_3 exon 37814617 37814850 37814617 ID=Msa0809960-mRNA-1:exon:11595;Parent=Msa0809960-mRNA-1
chr5_3 exon 37815380 37815437 37815380 ID=Msa0809960-mRNA-1:exon:11594;Parent=Msa0809960-mRNA-1
chr5_3 exon 37816091 37816416 37816091 ID=Msa0809960-mRNA-1:exon:11593;Parent=Msa0809960-mRNA-1
chr5_3 five_prime_UTR 37816091 37816416 37816091 ID=Msa0809960-mRNA-1:five_prime_utr;Parent=Msa0809960-mRNA-1
chr5_3 five_prime_UTR 37815380 37815437 37815380 ID=Msa0809960-mRNA-1:five_prime_utr;Parent=Msa0809960-mRNA-1
chr5_3 five_prime_UTR 37814617 37814850 37814617 ID=Msa0809960-mRNA-1:five_prime_utr;Parent=Msa0809960-mRNA-1
chr5_3 five_prime_UTR 37814209 37814327 37814209 ID=Msa0809960-mRNA-1:five_prime_utr;Parent=Msa0809960-mRNA-1
chr5_3 CDS 37814200 37814208 37814200 ID=Msa0809960-mRNA-1:cds;Parent=Msa0809960-mRNA-1
chr5_3 CDS 37813838 37813929 37813838 ID=Msa0809960-mRNA-1:cds;Parent=Msa0809960-mRNA-1
chr5_3 CDS 37813642 37813750 37813642 ID=Msa0809960-mRNA-1:cds;Parent=Msa0809960-mRNA-1
Gene Sequence

>Msa0809960

ATGTATCAGTGTACGTTGGAACGGCTGAGAGCGGAGGGAGTGAGGCTGAGTTGTTTGCGGCATGCTGTTTTTCGTGTAACTTCTTTCACGGGGGCTGTGCTTAGAGGAAGACGCAAAGGTAGAGGAAGGGTTTCCTTTCATCCACCTTCAACTTCACCTTCCATCCCCATTGAGGTGTATCATCGGAAGAAACTAAAATTGAGGGACTAA
Protein sequence

>Msa0809960

MYQCTLERLRAEGVRLSCLRHAVFRVTSFTGAVLRGRRKGRGRVSFHPPSTSPSIPIEVYHRKKLKLRD*