AlfalfaGEDB | Msa0820140

M. sativa cultivar zhongmu-4 / Msa0820140

KEGG: K21989
GO: -
PFAM: PF13967.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820140	sp\|Q9LVE4\|CSCL4_ARATH	75.737	746	178	2	1	743	1	746	0.0	1148

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820140	G7KCP6	99.086	766	7	0	1	766	1	766	0.0	1571

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002420	Msa0820140	0.834250	3.340306e-56	-8.615850e-47
Msa0276850	Msa0820140	0.818982	1.475024e-52	-8.615850e-47
Msa0276860	Msa0820140	0.830710	2.519218e-55	-8.615850e-47
Msa0309720	Msa0820140	0.825521	4.479726e-54	-8.615850e-47
Msa0416020	Msa0820140	0.811026	8.600385e-51	-8.615850e-47
Msa0508180	Msa0820140	0.804832	1.787560e-49	-8.615850e-47
Msa0574900	Msa0820140	0.800795	1.218514e-48	-8.615850e-47
Msa0716890	Msa0820140	0.808065	3.719078e-50	-8.615850e-47
Msa0739810	Msa0820140	0.970624	6.685126e-132	-8.615850e-47
Msa0758030	Msa0820140	0.845675	3.503240e-59	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820140	MtrunA17_Chr5g0439871	99.086	766	7	0	1	766	1	766	0.0	1571
Msa0820140	MtrunA17_Chr7g0259591	65.486	762	258	2	1	759	1	760	0.0	1050
Msa0820140	MtrunA17_Chr5g0409801	62.343	717	268	1	1	715	1	717	0.0	952
Msa0820140	MtrunA17_Chr4g0075301	72.500	520	139	2	1	517	1	519	0.0	780
Msa0820140	MtrunA17_Chr6g0453641	32.578	706	446	11	10	711	9	688	1.90e-138	425
Msa0820140	MtrunA17_Chr7g0217981	31.818	726	446	11	12	711	11	713	3.02e-129	402
Msa0820140	MtrunA17_Chr4g0042751	31.557	713	452	13	10	715	9	692	4.72e-123	385
Msa0820140	MtrunA17_Chr1g0152031	30.000	710	456	9	10	712	9	684	4.77e-118	373
Msa0820140	MtrunA17_Chr2g0285541	31.440	722	460	13	19	726	18	718	1.63e-114	363
Msa0820140	MtrunA17_Chr4g0075291	69.737	228	66	1	531	755	2	229	8.19e-112	339

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0820140	AT3G21620.1	75.737	746	178	2	1	743	1	746	1148
Msa0820140	AT1G11960.1	74.406	758	185	3	1	749	1	758	1147
Msa0820140	AT1G11960.3	74.406	758	185	3	1	749	1	758	1147
Msa0820140	AT4G22120.2	73.087	758	199	2	1	753	1	758	1136
Msa0820140	AT4G22120.4	73.087	758	199	2	1	753	1	758	1136
Msa0820140	AT4G22120.5	73.087	758	199	2	1	753	1	758	1136
Msa0820140	AT4G22120.1	73.087	758	199	2	1	753	1	758	1136
Msa0820140	AT4G22120.6	73.087	758	199	2	1	753	1	758	1136
Msa0820140	AT4G22120.3	73.087	758	199	2	1	753	1	758	1136
Msa0820140	AT4G15430.2	72.715	755	203	1	1	752	1	755	1133

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Find 182 sgRNAs with CRISPR-Local

Find 387 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGACCGTGAAGAAGCAA+TGG	0.595265	5_3:-57371259	Msa0820140:CDS
AAAATGCTAGGAGTGAAATG+GGG	0.730707	5_3:+57374760	None:intergenic
AAACAGGGTAATATGGCATA+AGG	0.496095	5_3:-57369953	Msa0820140:three_prime_UTR
AAACCCTTCATATACCATTT+TGG	0.198255	5_3:+57375766	None:intergenic
AAACTAGAAATCCAACAATA+TGG	0.385358	5_3:-57372732	Msa0820140:CDS
AAATACACCCTATCGTTTAT+AGG	0.291133	5_3:+57375790	None:intergenic
AAATCTTTCAAATTGAAGTT+TGG	0.330467	5_3:+57370422	None:intergenic
AACAATATGGTTGACTGAAT+GGG	0.447238	5_3:-57372719	Msa0820140:CDS
AACCAAGAATATGAGAGTGC+TGG	0.536287	5_3:-57370859	Msa0820140:CDS
AACCACATTTGAACGTTTCA+AGG	0.375878	5_3:+57374704	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAACAGAAAAAGAAGAGAA+TGG	+	chr5_3:57370042-57370061	None:intergenic	30.0%
GGTAATGTACTCTTATGTAA+TGG	-	chr5_3:57370113-57370132	Msa0820140:three_prime_UTR	30.0%
TGAGCTAGATTATGTGAAAA+GGG	-	chr5_3:57370449-57370468	Msa0820140:CDS	30.0%
AAAAAACTAGTAAAGTGGTG+GGG	+	chr5_3:57370565-57370584	None:intergenic	30.0%
GATTCAACCTATAAACGATA+GGG	-	chr5_3:57370920-57370939	Msa0820140:intron	30.0%
AAATACACCCTATCGTTTAT+AGG	+	chr5_3:57370930-57370949	None:intergenic	30.0%
AATGGTATATGAAGGGTTTA+AGG	-	chr5_3:57370955-57370974	Msa0820140:intron	30.0%
ATTGGTTAAAATCTACTCTC+TGG	+	chr5_3:57371213-57371232	None:intergenic	30.0%
AAGTAAGGACTAAACTTAGA+AGG	-	chr5_3:57371776-57371795	Msa0820140:CDS	30.0%
TCTTGAAACTAGAAAAACGT+AGG	+	chr5_3:57371863-57371882	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	57369890	57376849	57369890	ID=Msa0820140;Name=Msa0820140
chr5_3	mRNA	57369890	57376849	57369890	ID=Msa0820140-mRNA-1;Parent=Msa0820140;Name=Msa0820140-mRNA-1;_AED=0.07;_eAED=0.07;_QI=255\|1\|1\|1\|0.90\|0.83\|12\|339\|766
chr5_3	exon	57369890	57370483	57369890	ID=Msa0820140-mRNA-1:exon:17334;Parent=Msa0820140-mRNA-1
chr5_3	exon	57370663	57370896	57370663	ID=Msa0820140-mRNA-1:exon:17333;Parent=Msa0820140-mRNA-1
chr5_3	exon	57371113	57371437	57371113	ID=Msa0820140-mRNA-1:exon:17332;Parent=Msa0820140-mRNA-1
chr5_3	exon	57371567	57371812	57371567	ID=Msa0820140-mRNA-1:exon:17331;Parent=Msa0820140-mRNA-1
chr5_3	exon	57372523	57372863	57372523	ID=Msa0820140-mRNA-1:exon:17330;Parent=Msa0820140-mRNA-1
chr5_3	exon	57373187	57373267	57373187	ID=Msa0820140-mRNA-1:exon:17329;Parent=Msa0820140-mRNA-1
chr5_3	exon	57373407	57373526	57373407	ID=Msa0820140-mRNA-1:exon:17328;Parent=Msa0820140-mRNA-1
chr5_3	exon	57373931	57374032	57373931	ID=Msa0820140-mRNA-1:exon:17327;Parent=Msa0820140-mRNA-1
chr5_3	exon	57374187	57374322	57374187	ID=Msa0820140-mRNA-1:exon:17326;Parent=Msa0820140-mRNA-1
chr5_3	exon	57374651	57374797	57374651	ID=Msa0820140-mRNA-1:exon:17325;Parent=Msa0820140-mRNA-1
chr5_3	exon	57375592	57375931	57375592	ID=Msa0820140-mRNA-1:exon:17324;Parent=Msa0820140-mRNA-1
chr5_3	exon	57376621	57376849	57376621	ID=Msa0820140-mRNA-1:exon:17323;Parent=Msa0820140-mRNA-1
chr5_3	five_prime_UTR	57376621	57376849	57376621	ID=Msa0820140-mRNA-1:five_prime_utr;Parent=Msa0820140-mRNA-1
chr5_3	five_prime_UTR	57375906	57375931	57375906	ID=Msa0820140-mRNA-1:five_prime_utr;Parent=Msa0820140-mRNA-1
chr5_3	CDS	57375592	57375905	57375592	ID=Msa0820140-mRNA-1:cds;Parent=Msa0820140-mRNA-1
chr5_3	CDS	57374651	57374797	57374651	ID=Msa0820140-mRNA-1:cds;Parent=Msa0820140-mRNA-1
chr5_3	CDS	57374187	57374322	57374187	ID=Msa0820140-mRNA-1:cds;Parent=Msa0820140-mRNA-1
chr5_3	CDS	57373931	57374032	57373931	ID=Msa0820140-mRNA-1:cds;Parent=Msa0820140-mRNA-1
chr5_3	CDS	57373407	57373526	57373407	ID=Msa0820140-mRNA-1:cds;Parent=Msa0820140-mRNA-1
chr5_3	CDS	57373187	57373267	57373187	ID=Msa0820140-mRNA-1:cds;Parent=Msa0820140-mRNA-1
chr5_3	CDS	57372523	57372863	57372523	ID=Msa0820140-mRNA-1:cds;Parent=Msa0820140-mRNA-1
chr5_3	CDS	57371567	57371812	57371567	ID=Msa0820140-mRNA-1:cds;Parent=Msa0820140-mRNA-1
chr5_3	CDS	57371113	57371437	57371113	ID=Msa0820140-mRNA-1:cds;Parent=Msa0820140-mRNA-1
chr5_3	CDS	57370663	57370896	57370663	ID=Msa0820140-mRNA-1:cds;Parent=Msa0820140-mRNA-1
chr5_3	CDS	57370229	57370483	57370229	ID=Msa0820140-mRNA-1:cds;Parent=Msa0820140-mRNA-1
chr5_3	three_prime_UTR	57369890	57370228	57369890	ID=Msa0820140-mRNA-1:three_prime_utr;Parent=Msa0820140-mRNA-1

Gene Sequence

>Msa0820140

ATGGCTTCACTTGGCGATATAGGGCTTGCAGCTGCAATCAACATCCTTACTGCAATTGCATTCTTACTGGCATTTGCCATACTCCGGATTCAACCTATAAACGATAGGGTGTATTTTCCAAAATGGTATATGAAGGGTTTAAGGTCCAGTCCATTGCAAGGAGGGGCATTTGTGAGCAAATTTGTCAATATAGACTTCAGGTCATACATAAGGTTCTTGAACTGGATGCCTGCTGCATTGCAAATGCCAGAACCCGAACTAATCGAACATGCAGGCTTGGATTCTGCTGTATACTTGAGGATCTACTTACTCGGGCTGAAAATATTTGTCCCCATTTCACTCCTAGCATTTTCTGTTATGGTCCCTGTCAATTGGACCAATGACACCTTGAAACGTTCAAATGTGGTTTATAGCAGCATCGATAAGCTTTCAATTTCAAATATTCCACTTGGATCAAATAGATTTTGGACTCACTTGGTAATGGCTTATGCTTTTACCTTCTGGACATGCTATATCTTGAAAAGGGAGTATCAGATAGTTGCAGCAATGAGATTGTCTTTTCTTGCATCTGAAAGACGCCGTCCTGACCAATTCACGGTACTTGTTAGGAATGTACCGCCTGATGCTGATGAATCAGTCAGTGAGCTAGTTGAACATTTCTTTTTGGTGAACCATCCTGATCAATATCTAACTCATCAGGTTGTTTACGATGCAAAGAAACTCTCTAGTCTAGTTGCTAAGAAGAAGAAAAAACAGAATTGGCTTGACTACTATGAACTTAAATATTCTAGAAACGAATCCATAAGGCCAACTAAAAAGACTGGCTTTTTAGGTCTTTGTGGCAGTAAAGTGGATGCTATTGATTTTTATACTGCTGCAATTGAGAGACTATCAAGAGACATAGAGCTGGAGAAAGATAAGGTGACAAAGAATCCTAAATCTATAATGCCAGCAGCCTTTGTTTCCTTCAAAACTCGTTGGGGTGCTGCAGTTTGTGCACAAACTCAACAAACTAGAAATCCAACAATATGGTTGACTGAATGGGCTCCAGAGCCCCGTGACATATACTGGGATAACATGGCTATACCATATGTTTCTCTCTCAATAAGGAGACTTGTAATTGGTGTTGCGTTCTTCTTTCTTACATTCTTTTTCATGATTCCCATTGCATTCGTACAGTCACTTGCTAATATAGAAGGCATTGAAAAAGCCGCACCATTCCTCAAGTCCATTATTGAAATCGACGTCATCAAGTCTTTTATACAAGGTTTCCTTCCTGGGATTGCTTTGAAGCTATTCCTCATTTTTTTGCCAACAATATTGATGATCATGTCCAAGTTCGAAGGGTTTATAAGTCAGTCTTCTCTGGAAAGAAGATGCGCCTCAAGATATTACATCTTCCAGTTCATTAATGTGTTTCTGGGGAGCATAATTACCGGGACTGCATTCCAACAATTAGATAAATTCATTCATCAGTCTGCAAATGAAATTCCAAAGACAATTGGTGTTTCGATTCCAATGAAAGCAACTTTCTTCATAACATACATAATGGTTGATGGATGGGCAGGATGTGCTGGTGAGATTTTAAGGTTGAAGCCTTTGATATTTTATCACTTAAAGAATTTCTTGTTGGTGAAGACTGAAAAAGACCGTGAAGAAGCAATGGATCCTGGGACTATTGGTTTCAATACAGGAGAACCTCAGATACAACTTTATTTCTTGCTTGGCCTTGTGTATGCTGTAGTCACACCATTTCTACTTCCATACATCATTGTCTTTTTTGGTTTGGCATATCTTGTCTACCGTCATCAGATTATAAACGTGTACAACCAAGAATATGAGAGTGCTGGAGCATTCTGGCCTGATGTCCATGGACGTATTGTATTTGCATTAGTTGTCTCACAACTTCTGTTAATGGGATTATTGAGTACAAAAGAAGCTGCTAATTCGACTCCATTACTCATCGCTCTCCCAGTTTTGACAATATGGTTTCATCGGTTTTGCAAGGGAAGCTACGAACCTGCTTTTACTACTCATCCATTACAGGAAGCAATGGTCAAAGACACATTGGAGCGTACTAAAGAGCCAAACTTCAATTTGAAAGATTTCCTCCATGATGCATATATCCATCCCGTTTTCAAAGATGACGGAGACACAGACAGTGATGTGATGAGTCAAGAGTGGAAGGAAGAGCCGGTAATTGTCCAAACAAAACGCCAGTCGCGGAAGAACACACCTGCGCCTAGCAAACATAGTGGTGGCTCATTACAAGCCTCTATGCATGGCACTACTGATGTCTGA

Protein sequence

>Msa0820140

MASLGDIGLAAAINILTAIAFLLAFAILRIQPINDRVYFPKWYMKGLRSSPLQGGAFVSKFVNIDFRSYIRFLNWMPAALQMPEPELIEHAGLDSAVYLRIYLLGLKIFVPISLLAFSVMVPVNWTNDTLKRSNVVYSSIDKLSISNIPLGSNRFWTHLVMAYAFTFWTCYILKREYQIVAAMRLSFLASERRRPDQFTVLVRNVPPDADESVSELVEHFFLVNHPDQYLTHQVVYDAKKLSSLVAKKKKKQNWLDYYELKYSRNESIRPTKKTGFLGLCGSKVDAIDFYTAAIERLSRDIELEKDKVTKNPKSIMPAAFVSFKTRWGAAVCAQTQQTRNPTIWLTEWAPEPRDIYWDNMAIPYVSLSIRRLVIGVAFFFLTFFFMIPIAFVQSLANIEGIEKAAPFLKSIIEIDVIKSFIQGFLPGIALKLFLIFLPTILMIMSKFEGFISQSSLERRCASRYYIFQFINVFLGSIITGTAFQQLDKFIHQSANEIPKTIGVSIPMKATFFITYIMVDGWAGCAGEILRLKPLIFYHLKNFLLVKTEKDREEAMDPGTIGFNTGEPQIQLYFLLGLVYAVVTPFLLPYIIVFFGLAYLVYRHQIINVYNQEYESAGAFWPDVHGRIVFALVVSQLLLMGLLSTKEAANSTPLLIALPVLTIWFHRFCKGSYEPAFTTHPLQEAMVKDTLERTKEPNFNLKDFLHDAYIHPVFKDDGDTDSDVMSQEWKEEPVIVQTKRQSRKNTPAPSKHSGGSLQASMHGTTDV*