AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0829560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0829560 MtrunA17_Chr2g0281121 93.103 261 14 2 1 260 14 271 2.67e-167 463
Msa0829560 MtrunA17_Chr4g0048371 53.532 269 92 10 1 249 24 279 1.05e-47 159
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0829560 AT4G21500.1 34.568 243 101 7 17 240 12 215 1.09e-17 79.7

Find 41 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAAGTTGAAATCTAAAA+TGG 0.203889 5_4:+2574167 Msa0829560:CDS
TCGAGACCCACTTGAAAATT+TGG 0.233268 5_4:-2574134 None:intergenic
CACCGGAGAATATGAATTTC+CGG 0.299531 5_4:+2574406 Msa0829560:CDS
GAACTATCTGAATCAGATTC+AGG 0.336496 5_4:-2574345 None:intergenic
TTTCTTCACTCTTAGATCTC+TGG 0.338598 5_4:-2574014 None:intergenic
TTCATCGGAGAATGAGTTTC+CGG 0.350752 5_4:+2574365 Msa0829560:CDS
AGTTTCCGGCGATGGAGAAC+TGG 0.362497 5_4:+2574379 Msa0829560:CDS
GGCTTTAAGAGGAAGGCTTT+GGG 0.375043 5_4:+2574474 Msa0829560:CDS
TGGCTTTAAGAGGAAGGCTT+TGG 0.384066 5_4:+2574473 Msa0829560:CDS
GAGATCTAAGAGTGAAGAAA+AGG 0.411423 5_4:+2574017 Msa0829560:CDS
TTAATTGAGATAGCTCTTGA+TGG 0.421762 5_4:+2574453 Msa0829560:CDS
TCTAATGAGAATTGTGTTGC+TGG 0.422953 5_4:+2574288 Msa0829560:CDS
ACATTTCTCCGACGAAGCGC+AGG 0.439702 5_4:+2574532 Msa0829560:CDS
TAGCTCTTGATGGCTTTAAG+AGG 0.465176 5_4:+2574463 Msa0829560:CDS
TCACCAGTTCTAATTCTCAC+TGG 0.478806 5_4:+2573955 Msa0829560:CDS
GGAGAATGAGTTTCCGGCGA+TGG 0.484875 5_4:+2574371 Msa0829560:CDS
GAAAAGTTGAAATCTAAAAT+GGG 0.488091 5_4:+2574168 Msa0829560:CDS
CTCTATCTTCTTCATCCCAC+CGG 0.498324 5_4:-2574425 None:intergenic
GGCTTTGGGATTTCAGTTTG+AGG 0.516822 5_4:+2574488 Msa0829560:CDS
CACCGGAAATTCATATTCTC+CGG 0.520892 5_4:-2574408 None:intergenic
ATGGAACGTGAAAGCGCCGT+TGG 0.537116 5_4:+2574230 Msa0829560:CDS
GAAAACTCCCTGCGCTTCGT+CGG 0.545300 5_4:-2574540 None:intergenic
TCTTGATGGCTTTAAGAGGA+AGG 0.545888 5_4:+2574467 Msa0829560:CDS
GGGATGAAGAAGATAGAGAA+GGG 0.548429 5_4:+2574430 Msa0829560:CDS
GATGGAGAACTGGGACTCAC+CGG 0.552428 5_4:+2574389 Msa0829560:CDS
ACACCAGTGAGAATTAGAAC+TGG 0.561591 5_4:-2573958 None:intergenic
GAGTCCCAGTTCTCCATCGC+CGG 0.564533 5_4:-2574384 None:intergenic
GATGAGAATCAAGGTGCAGA+AGG 0.566008 5_4:-2573899 None:intergenic
TCTTCATATATAACTTCCAA+CGG 0.567961 5_4:-2574246 None:intergenic
AGGAAAATAGATGAGAATCA+AGG 0.575079 5_4:-2573908 None:intergenic
GTTTCCGGCGATGGAGAACT+GGG 0.578488 5_4:+2574380 Msa0829560:CDS
GGAAAACACCTCCTCCTCGC+CGG 0.581509 5_4:-2574562 None:intergenic
TTAATTCCAACTCAAATCAC+CGG 0.596284 5_4:-2574583 None:intergenic
CATTTCTCCGACGAAGCGCA+GGG 0.607667 5_4:+2574533 Msa0829560:CDS
TGGGATGAAGAAGATAGAGA+AGG 0.615054 5_4:+2574429 Msa0829560:CDS
CGGAGAATATGAATTTCCGG+TGG 0.615835 5_4:+2574409 Msa0829560:CDS
ATCTGATTCAGATAGTTCAT+CGG 0.620524 5_4:+2574350 Msa0829560:CDS
ATATGAAGATGAAGAAACAG+AGG 0.620651 5_4:+2574260 Msa0829560:CDS
GGGGAAAACAGAGGAAAATG+TGG 0.620751 5_4:+2574095 Msa0829560:CDS
GGAGAATATGAATTTCCGGT+GGG 0.667099 5_4:+2574410 Msa0829560:CDS
GTAATAATGCATGAAAACAG+AGG 0.676002 5_4:-2573877 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAGATTTCAACTTTTCTT+CGG - chr5_4:2574166-2574185 None:intergenic 20.0%
!! AGAAAAGTTGAAATCTAAAA+TGG + chr5_4:2574167-2574186 Msa0829560:CDS 20.0%
!!! GAAAAGTTGAAATCTAAAAT+GGG + chr5_4:2574168-2574187 Msa0829560:CDS 20.0%
!!! TTTAGATTTCAACTTTTCTT+CGG - chr5_4:2574166-2574185 None:intergenic 20.0%
!! AGAAAAGTTGAAATCTAAAA+TGG + chr5_4:2574167-2574186 Msa0829560:CDS 20.0%
!!! GAAAAGTTGAAATCTAAAAT+GGG + chr5_4:2574168-2574187 Msa0829560:CDS 20.0%
!! ACTATTTTGCAGAATGAATA+AGG - chr5_4:2573931-2573950 None:intergenic 25.0%
!! TTGATTTTTGTACTTCGTTT+TGG + chr5_4:2573985-2574004 Msa0829560:CDS 25.0%
! AGTGAAGAAAAGGAAAAATT+TGG + chr5_4:2574027-2574046 Msa0829560:CDS 25.0%
! TCTTCATATATAACTTCCAA+CGG - chr5_4:2574249-2574268 None:intergenic 25.0%
!! ACTATTTTGCAGAATGAATA+AGG - chr5_4:2573931-2573950 None:intergenic 25.0%
!! TTGATTTTTGTACTTCGTTT+TGG + chr5_4:2573985-2574004 Msa0829560:CDS 25.0%
! AGTGAAGAAAAGGAAAAATT+TGG + chr5_4:2574027-2574046 Msa0829560:CDS 25.0%
! TCTTCATATATAACTTCCAA+CGG - chr5_4:2574249-2574268 None:intergenic 25.0%
GTAATAATGCATGAAAACAG+AGG - chr5_4:2573880-2573899 None:intergenic 30.0%
AGGAAAATAGATGAGAATCA+AGG - chr5_4:2573911-2573930 None:intergenic 30.0%
! TTCATCTCTGTTTTCTTCAT+TGG - chr5_4:2574070-2574089 None:intergenic 30.0%
AGAAAACAGAGATGAAAAAC+AGG + chr5_4:2574074-2574093 Msa0829560:CDS 30.0%
GAAAACAGAGATGAAAAACA+GGG + chr5_4:2574075-2574094 Msa0829560:CDS 30.0%
AAAACAGAGATGAAAAACAG+GGG + chr5_4:2574076-2574095 Msa0829560:CDS 30.0%
!!! TCTTGACCAAATTTTCAAGT+GGG + chr5_4:2574128-2574147 Msa0829560:CDS 30.0%
! TTGATGAGTCTTTTGTTGAA+TGG + chr5_4:2574211-2574230 Msa0829560:CDS 30.0%
ATATGAAGATGAAGAAACAG+AGG + chr5_4:2574260-2574279 Msa0829560:CDS 30.0%
ATCTGATTCAGATAGTTCAT+CGG + chr5_4:2574350-2574369 Msa0829560:CDS 30.0%
TTAATTGAGATAGCTCTTGA+TGG + chr5_4:2574453-2574472 Msa0829560:CDS 30.0%
TTAATTCCAACTCAAATCAC+CGG - chr5_4:2574586-2574605 None:intergenic 30.0%
GTAATAATGCATGAAAACAG+AGG - chr5_4:2573880-2573899 None:intergenic 30.0%
AGGAAAATAGATGAGAATCA+AGG - chr5_4:2573911-2573930 None:intergenic 30.0%
! TTCATCTCTGTTTTCTTCAT+TGG - chr5_4:2574070-2574089 None:intergenic 30.0%
AGAAAACAGAGATGAAAAAC+AGG + chr5_4:2574074-2574093 Msa0829560:CDS 30.0%
GAAAACAGAGATGAAAAACA+GGG + chr5_4:2574075-2574094 Msa0829560:CDS 30.0%
AAAACAGAGATGAAAAACAG+GGG + chr5_4:2574076-2574095 Msa0829560:CDS 30.0%
!!! TCTTGACCAAATTTTCAAGT+GGG + chr5_4:2574128-2574147 Msa0829560:CDS 30.0%
! TTGATGAGTCTTTTGTTGAA+TGG + chr5_4:2574211-2574230 Msa0829560:CDS 30.0%
ATATGAAGATGAAGAAACAG+AGG + chr5_4:2574260-2574279 Msa0829560:CDS 30.0%
ATCTGATTCAGATAGTTCAT+CGG + chr5_4:2574350-2574369 Msa0829560:CDS 30.0%
TTAATTGAGATAGCTCTTGA+TGG + chr5_4:2574453-2574472 Msa0829560:CDS 30.0%
TTAATTCCAACTCAAATCAC+CGG - chr5_4:2574586-2574605 None:intergenic 30.0%
TTTCTTCACTCTTAGATCTC+TGG - chr5_4:2574017-2574036 None:intergenic 35.0%
GAGATCTAAGAGTGAAGAAA+AGG + chr5_4:2574017-2574036 Msa0829560:CDS 35.0%
!! CTCTTGACCAAATTTTCAAG+TGG + chr5_4:2574127-2574146 Msa0829560:CDS 35.0%
TCTAATGAGAATTGTGTTGC+TGG + chr5_4:2574288-2574307 Msa0829560:CDS 35.0%
GAACTATCTGAATCAGATTC+AGG - chr5_4:2574348-2574367 None:intergenic 35.0%
TTTCTTCACTCTTAGATCTC+TGG - chr5_4:2574017-2574036 None:intergenic 35.0%
GAGATCTAAGAGTGAAGAAA+AGG + chr5_4:2574017-2574036 Msa0829560:CDS 35.0%
!! CTCTTGACCAAATTTTCAAG+TGG + chr5_4:2574127-2574146 Msa0829560:CDS 35.0%
TCTAATGAGAATTGTGTTGC+TGG + chr5_4:2574288-2574307 Msa0829560:CDS 35.0%
GAACTATCTGAATCAGATTC+AGG - chr5_4:2574348-2574367 None:intergenic 35.0%
! TCACCAGTTCTAATTCTCAC+TGG + chr5_4:2573955-2573974 Msa0829560:CDS 40.0%
ACACCAGTGAGAATTAGAAC+TGG - chr5_4:2573961-2573980 None:intergenic 40.0%
TGAAAAACAGGGGAAAACAG+AGG + chr5_4:2574086-2574105 Msa0829560:CDS 40.0%
TCGAGACCCACTTGAAAATT+TGG - chr5_4:2574137-2574156 None:intergenic 40.0%
TTCATCGGAGAATGAGTTTC+CGG + chr5_4:2574365-2574384 Msa0829560:CDS 40.0%
CACCGGAGAATATGAATTTC+CGG + chr5_4:2574406-2574425 Msa0829560:CDS 40.0%
CACCGGAAATTCATATTCTC+CGG - chr5_4:2574411-2574430 None:intergenic 40.0%
GGAGAATATGAATTTCCGGT+GGG + chr5_4:2574410-2574429 Msa0829560:CDS 40.0%
TGGGATGAAGAAGATAGAGA+AGG + chr5_4:2574429-2574448 Msa0829560:CDS 40.0%
GGGATGAAGAAGATAGAGAA+GGG + chr5_4:2574430-2574449 Msa0829560:CDS 40.0%
!! TAGCTCTTGATGGCTTTAAG+AGG + chr5_4:2574463-2574482 Msa0829560:CDS 40.0%
!! TCTTGATGGCTTTAAGAGGA+AGG + chr5_4:2574467-2574486 Msa0829560:CDS 40.0%
! TGTTTTCCGGTGATTTGAGT+TGG + chr5_4:2574577-2574596 Msa0829560:CDS 40.0%
! TCACCAGTTCTAATTCTCAC+TGG + chr5_4:2573955-2573974 Msa0829560:CDS 40.0%
ACACCAGTGAGAATTAGAAC+TGG - chr5_4:2573961-2573980 None:intergenic 40.0%
TGAAAAACAGGGGAAAACAG+AGG + chr5_4:2574086-2574105 Msa0829560:CDS 40.0%
TCGAGACCCACTTGAAAATT+TGG - chr5_4:2574137-2574156 None:intergenic 40.0%
TTCATCGGAGAATGAGTTTC+CGG + chr5_4:2574365-2574384 Msa0829560:CDS 40.0%
CACCGGAGAATATGAATTTC+CGG + chr5_4:2574406-2574425 Msa0829560:CDS 40.0%
CACCGGAAATTCATATTCTC+CGG - chr5_4:2574411-2574430 None:intergenic 40.0%
GGAGAATATGAATTTCCGGT+GGG + chr5_4:2574410-2574429 Msa0829560:CDS 40.0%
TGGGATGAAGAAGATAGAGA+AGG + chr5_4:2574429-2574448 Msa0829560:CDS 40.0%
GGGATGAAGAAGATAGAGAA+GGG + chr5_4:2574430-2574449 Msa0829560:CDS 40.0%
!! TAGCTCTTGATGGCTTTAAG+AGG + chr5_4:2574463-2574482 Msa0829560:CDS 40.0%
!! TCTTGATGGCTTTAAGAGGA+AGG + chr5_4:2574467-2574486 Msa0829560:CDS 40.0%
! TGTTTTCCGGTGATTTGAGT+TGG + chr5_4:2574577-2574596 Msa0829560:CDS 40.0%
GATGAGAATCAAGGTGCAGA+AGG - chr5_4:2573902-2573921 None:intergenic 45.0%
GGGGAAAACAGAGGAAAATG+TGG + chr5_4:2574095-2574114 Msa0829560:CDS 45.0%
CGGAGAATATGAATTTCCGG+TGG + chr5_4:2574409-2574428 Msa0829560:CDS 45.0%
CTCTATCTTCTTCATCCCAC+CGG - chr5_4:2574428-2574447 None:intergenic 45.0%
!! TGGCTTTAAGAGGAAGGCTT+TGG + chr5_4:2574473-2574492 Msa0829560:CDS 45.0%
!! GGCTTTAAGAGGAAGGCTTT+GGG + chr5_4:2574474-2574493 Msa0829560:CDS 45.0%
!! GGCTTTGGGATTTCAGTTTG+AGG + chr5_4:2574488-2574507 Msa0829560:CDS 45.0%
GATGAGAATCAAGGTGCAGA+AGG - chr5_4:2573902-2573921 None:intergenic 45.0%
GGGGAAAACAGAGGAAAATG+TGG + chr5_4:2574095-2574114 Msa0829560:CDS 45.0%
CGGAGAATATGAATTTCCGG+TGG + chr5_4:2574409-2574428 Msa0829560:CDS 45.0%
CTCTATCTTCTTCATCCCAC+CGG - chr5_4:2574428-2574447 None:intergenic 45.0%
!! TGGCTTTAAGAGGAAGGCTT+TGG + chr5_4:2574473-2574492 Msa0829560:CDS 45.0%
!! GGCTTTAAGAGGAAGGCTTT+GGG + chr5_4:2574474-2574493 Msa0829560:CDS 45.0%
!! GGCTTTGGGATTTCAGTTTG+AGG + chr5_4:2574488-2574507 Msa0829560:CDS 45.0%
!! ATGGAACGTGAAAGCGCCGT+TGG + chr5_4:2574230-2574249 Msa0829560:CDS 55.0%
GGAGAATGAGTTTCCGGCGA+TGG + chr5_4:2574371-2574390 Msa0829560:CDS 55.0%
AGTTTCCGGCGATGGAGAAC+TGG + chr5_4:2574379-2574398 Msa0829560:CDS 55.0%
GTTTCCGGCGATGGAGAACT+GGG + chr5_4:2574380-2574399 Msa0829560:CDS 55.0%
GATGGAGAACTGGGACTCAC+CGG + chr5_4:2574389-2574408 Msa0829560:CDS 55.0%
ACATTTCTCCGACGAAGCGC+AGG + chr5_4:2574532-2574551 Msa0829560:CDS 55.0%
CATTTCTCCGACGAAGCGCA+GGG + chr5_4:2574533-2574552 Msa0829560:CDS 55.0%
GAAAACTCCCTGCGCTTCGT+CGG - chr5_4:2574543-2574562 None:intergenic 55.0%
! ACGAAGCGCAGGGAGTTTTC+CGG + chr5_4:2574543-2574562 Msa0829560:CDS 55.0%
!! ATGGAACGTGAAAGCGCCGT+TGG + chr5_4:2574230-2574249 Msa0829560:CDS 55.0%
GGAGAATGAGTTTCCGGCGA+TGG + chr5_4:2574371-2574390 Msa0829560:CDS 55.0%
AGTTTCCGGCGATGGAGAAC+TGG + chr5_4:2574379-2574398 Msa0829560:CDS 55.0%
GTTTCCGGCGATGGAGAACT+GGG + chr5_4:2574380-2574399 Msa0829560:CDS 55.0%
GATGGAGAACTGGGACTCAC+CGG + chr5_4:2574389-2574408 Msa0829560:CDS 55.0%
ACATTTCTCCGACGAAGCGC+AGG + chr5_4:2574532-2574551 Msa0829560:CDS 55.0%
CATTTCTCCGACGAAGCGCA+GGG + chr5_4:2574533-2574552 Msa0829560:CDS 55.0%
GAAAACTCCCTGCGCTTCGT+CGG - chr5_4:2574543-2574562 None:intergenic 55.0%
! ACGAAGCGCAGGGAGTTTTC+CGG + chr5_4:2574543-2574562 Msa0829560:CDS 55.0%
GAGTCCCAGTTCTCCATCGC+CGG - chr5_4:2574387-2574406 None:intergenic 60.0%
GGAAAACACCTCCTCCTCGC+CGG - chr5_4:2574565-2574584 None:intergenic 60.0%
!!! GGCGAGGAGGAGGTGTTTTC+CGG + chr5_4:2574564-2574583 Msa0829560:CDS 60.0%
GAGTCCCAGTTCTCCATCGC+CGG - chr5_4:2574387-2574406 None:intergenic 60.0%
GGAAAACACCTCCTCCTCGC+CGG - chr5_4:2574565-2574584 None:intergenic 60.0%
!!! GGCGAGGAGGAGGTGTTTTC+CGG + chr5_4:2574564-2574583 Msa0829560:CDS 60.0%
! CAGGGAGTTTTCCGGCGAGG+AGG + chr5_4:2574551-2574570 Msa0829560:CDS 65.0%
! GGAGTTTTCCGGCGAGGAGG+AGG + chr5_4:2574554-2574573 Msa0829560:CDS 65.0%
! CAGGGAGTTTTCCGGCGAGG+AGG + chr5_4:2574551-2574570 Msa0829560:CDS 65.0%
! GGAGTTTTCCGGCGAGGAGG+AGG + chr5_4:2574554-2574573 Msa0829560:CDS 65.0%
! GCGCAGGGAGTTTTCCGGCG+AGG + chr5_4:2574548-2574567 Msa0829560:CDS 70.0%
! GCGCAGGGAGTTTTCCGGCG+AGG + chr5_4:2574548-2574567 Msa0829560:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 2573823 2574605 2573823 ID=Msa0829560;Name=Msa0829560
chr5_4 mRNA 2573823 2574605 2573823 ID=Msa0829560-mRNA-1;Parent=Msa0829560;Name=Msa0829560-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|260
chr5_4 exon 2573823 2574605 2573823 ID=Msa0829560-mRNA-1:exon:845;Parent=Msa0829560-mRNA-1
chr5_4 CDS 2573823 2574605 2573823 ID=Msa0829560-mRNA-1:cds;Parent=Msa0829560-mRNA-1
Gene Sequence

>Msa0829560

ATGAACTTGTTATGCAAAGAAACTTTTAACTTTCTCTCAATTGTTTCTTCAAATCCTCTGTTTTCATGCATTATTACCTTCTGCACCTTGATTCTCATCTATTTTCCTTATTCATTCTGCAAAATAGTTTTCTCACCAGTTCTAATTCTCACTGGTGTTATATTGATTTTTGTACTTCGTTTTGGTGCAATCCAGAGATCTAAGAGTGAAGAAAAGGAAAAATTTGGTGAATTAGAATCAGTTACCAATGAAGAAAACAGAGATGAAAAACAGGGGAAAACAGAGGAAAATGTGGAAAATGAGTCTCTTGACCAAATTTTCAAGTGGGTCTCGAGAAATTCCGAAGAAAAGTTGAAATCTAAAATGGGTTTTGAATCAAATTCGTTTCTTGATGAGTCTTTTGTTGAATGGAACGTGAAAGCGCCGTTGGAAGTTATATATGAAGATGAAGAAACAGAGGATATTTCTAATGAGAATTGTGTTGCTGGAATTTTGAGACACTCTTCATTGTCACGTTATTATCCTGAATCTGATTCAGATAGTTCATCGGAGAATGAGTTTCCGGCGATGGAGAACTGGGACTCACCGGAGAATATGAATTTCCGGTGGGATGAAGAAGATAGAGAAGGGTTAATTGAGATAGCTCTTGATGGCTTTAAGAGGAAGGCTTTGGGATTTCAGTTTGAGGAAGAGAATATGATTGAGATTGACATTTCTCCGACGAAGCGCAGGGAGTTTTCCGGCGAGGAGGAGGTGTTTTCCGGTGATTTGAGTTGGAATTAA

Protein sequence

>Msa0829560

MNLLCKETFNFLSIVSSNPLFSCIITFCTLILIYFPYSFCKIVFSPVLILTGVILIFVLRFGAIQRSKSEEKEKFGELESVTNEENRDEKQGKTEENVENESLDQIFKWVSRNSEEKLKSKMGFESNSFLDESFVEWNVKAPLEVIYEDEETEDISNENCVAGILRHSSLSRYYPESDSDSSSENEFPAMENWDSPENMNFRWDEEDREGLIEIALDGFKRKALGFQFEEENMIEIDISPTKRREFSGEEEVFSGDLSWN*