AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0311920


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0311920 MtrunA17_Chr2g0281121 93.103 261 14 2 1 260 14 271 1.05e-166 461
Msa0311920 MtrunA17_Chr4g0048371 52.788 269 94 10 1 249 24 279 4.62e-47 158
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0311920 AT4G21500.1 34.979 243 100 7 17 240 12 215 4.05e-18 80.9

Find 42 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAAGTTGAAATCTAAAA+TGG 0.203889 2_4:-6883073 Msa0311920:CDS
TCGAGACCCACTTGAAAATT+TGG 0.233268 2_4:+6883106 None:intergenic
CACCGGAGAATATGAATTTC+CGG 0.299531 2_4:-6882834 Msa0311920:CDS
GAACTATCTGAATCAGATTC+AGG 0.336496 2_4:+6882895 None:intergenic
TTTCTTCACTCTTAGATCTC+TGG 0.338598 2_4:+6883226 None:intergenic
TTCATCGGAGAATGAGTTTC+CGG 0.350752 2_4:-6882875 Msa0311920:CDS
AGTTTCCGGCGATGGAGAAC+TGG 0.362497 2_4:-6882861 Msa0311920:CDS
AATATAACACCAGTGAGAAT+TGG 0.374103 2_4:+6883276 None:intergenic
GGCTTTAAGAGGAAGGCTTT+GGG 0.375043 2_4:-6882766 Msa0311920:CDS
TGGCTTTAAGAGGAAGGCTT+TGG 0.384066 2_4:-6882767 Msa0311920:CDS
GAGATCTAAGAGTGAAGAAA+AGG 0.411423 2_4:-6883223 Msa0311920:CDS
TTAATTGAGATAGCTCTTGA+TGG 0.421762 2_4:-6882787 Msa0311920:CDS
TCTAATGAGAATTGTGTTGC+TGG 0.422953 2_4:-6882952 Msa0311920:CDS
ACATTTCTCCGACGAAGCGC+AGG 0.439702 2_4:-6882708 Msa0311920:CDS
TAGCTCTTGATGGCTTTAAG+AGG 0.465176 2_4:-6882777 Msa0311920:CDS
TCACCAGTTCCAATTCTCAC+TGG 0.476880 2_4:-6883285 Msa0311920:CDS
GGAGAATGAGTTTCCGGCGA+TGG 0.484875 2_4:-6882869 Msa0311920:CDS
GAAAAGTTGAAATCTAAAAT+GGG 0.488091 2_4:-6883072 Msa0311920:CDS
CTCTATCTTCTTCATCCCAC+CGG 0.498324 2_4:+6882815 None:intergenic
GGCTTTGGGATTTCAGTTTG+AGG 0.516822 2_4:-6882752 Msa0311920:CDS
CACCGGAAATTCATATTCTC+CGG 0.520892 2_4:+6882832 None:intergenic
ATGGAACGTGAAAGCGCCGT+TGG 0.537116 2_4:-6883010 Msa0311920:CDS
GAAAACTCCCTGCGCTTCGT+CGG 0.545300 2_4:+6882700 None:intergenic
TCTTGATGGCTTTAAGAGGA+AGG 0.545888 2_4:-6882773 Msa0311920:CDS
GGGATGAAGAAGATAGAGAA+GGG 0.548429 2_4:-6882810 Msa0311920:CDS
GATGGAGAACTGGGACTCAC+CGG 0.552428 2_4:-6882851 Msa0311920:CDS
ACACCAGTGAGAATTGGAAC+TGG 0.559238 2_4:+6883282 None:intergenic
GAGTCCCAGTTCTCCATCGC+CGG 0.564533 2_4:+6882856 None:intergenic
GATGAGAATCAAGGTGCAGA+AGG 0.566008 2_4:+6883341 None:intergenic
TCTTCATATATAACTTCCAA+CGG 0.567961 2_4:+6882994 None:intergenic
AGGAAAATAGATGAGAATCA+AGG 0.575079 2_4:+6883332 None:intergenic
GTTTCCGGCGATGGAGAACT+GGG 0.578488 2_4:-6882860 Msa0311920:CDS
GGAAAACACCTCCTCCTCGC+CGG 0.581509 2_4:+6882678 None:intergenic
TTAATTCCAACTCAAATCAC+CGG 0.596284 2_4:+6882657 None:intergenic
CATTTCTCCGACGAAGCGCA+GGG 0.607667 2_4:-6882707 Msa0311920:CDS
TGGGATGAAGAAGATAGAGA+AGG 0.615054 2_4:-6882811 Msa0311920:CDS
CGGAGAATATGAATTTCCGG+TGG 0.615835 2_4:-6882831 Msa0311920:CDS
ATCTGATTCAGATAGTTCAT+CGG 0.620524 2_4:-6882890 Msa0311920:CDS
ATATGAAGATGAAGAAACAG+AGG 0.620651 2_4:-6882980 Msa0311920:CDS
GGGGAAAACAGAGGAAAATG+TGG 0.620751 2_4:-6883145 Msa0311920:CDS
GGAGAATATGAATTTCCGGT+GGG 0.667099 2_4:-6882830 Msa0311920:CDS
GTAATAATGCATGAAAACAG+AGG 0.676002 2_4:+6883363 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAGATTTCAACTTTTCTT+CGG + chr2_4:6883000-6883019 None:intergenic 20.0%
!! AGAAAAGTTGAAATCTAAAA+TGG - chr2_4:6883001-6883020 Msa0311920:CDS 20.0%
!!! GAAAAGTTGAAATCTAAAAT+GGG - chr2_4:6883002-6883021 Msa0311920:CDS 20.0%
!!! TTTAGATTTCAACTTTTCTT+CGG + chr2_4:6883000-6883019 None:intergenic 20.0%
!! AGAAAAGTTGAAATCTAAAA+TGG - chr2_4:6883001-6883020 Msa0311920:CDS 20.0%
!!! GAAAAGTTGAAATCTAAAAT+GGG - chr2_4:6883002-6883021 Msa0311920:CDS 20.0%
!! ACTATTTTGCAGAATGAATA+AGG + chr2_4:6882765-6882784 None:intergenic 25.0%
!! TTGATTTTTGTACTTCGTTT+TGG - chr2_4:6882819-6882838 Msa0311920:CDS 25.0%
! AGTGAAGAAAAGGAAAAATT+TGG - chr2_4:6882861-6882880 Msa0311920:CDS 25.0%
! TCTTCATATATAACTTCCAA+CGG + chr2_4:6883083-6883102 None:intergenic 25.0%
!! ACTATTTTGCAGAATGAATA+AGG + chr2_4:6882765-6882784 None:intergenic 25.0%
!! TTGATTTTTGTACTTCGTTT+TGG - chr2_4:6882819-6882838 Msa0311920:CDS 25.0%
! AGTGAAGAAAAGGAAAAATT+TGG - chr2_4:6882861-6882880 Msa0311920:CDS 25.0%
! TCTTCATATATAACTTCCAA+CGG + chr2_4:6883083-6883102 None:intergenic 25.0%
GTAATAATGCATGAAAACAG+AGG + chr2_4:6882714-6882733 None:intergenic 30.0%
AGGAAAATAGATGAGAATCA+AGG + chr2_4:6882745-6882764 None:intergenic 30.0%
AATATAACACCAGTGAGAAT+TGG + chr2_4:6882801-6882820 None:intergenic 30.0%
! TTCATCTCTGTTTTCTTCAT+TGG + chr2_4:6882904-6882923 None:intergenic 30.0%
AGAAAACAGAGATGAAAAAC+AGG - chr2_4:6882908-6882927 Msa0311920:CDS 30.0%
GAAAACAGAGATGAAAAACA+GGG - chr2_4:6882909-6882928 Msa0311920:CDS 30.0%
AAAACAGAGATGAAAAACAG+GGG - chr2_4:6882910-6882929 Msa0311920:CDS 30.0%
!!! TCTTGACCAAATTTTCAAGT+GGG - chr2_4:6882962-6882981 Msa0311920:CDS 30.0%
! TTGATGAGTCTTTTGTTGAA+TGG - chr2_4:6883045-6883064 Msa0311920:CDS 30.0%
ATATGAAGATGAAGAAACAG+AGG - chr2_4:6883094-6883113 Msa0311920:CDS 30.0%
ATCTGATTCAGATAGTTCAT+CGG - chr2_4:6883184-6883203 Msa0311920:CDS 30.0%
TTAATTGAGATAGCTCTTGA+TGG - chr2_4:6883287-6883306 Msa0311920:CDS 30.0%
TTAATTCCAACTCAAATCAC+CGG + chr2_4:6883420-6883439 None:intergenic 30.0%
GTAATAATGCATGAAAACAG+AGG + chr2_4:6882714-6882733 None:intergenic 30.0%
AGGAAAATAGATGAGAATCA+AGG + chr2_4:6882745-6882764 None:intergenic 30.0%
AATATAACACCAGTGAGAAT+TGG + chr2_4:6882801-6882820 None:intergenic 30.0%
! TTCATCTCTGTTTTCTTCAT+TGG + chr2_4:6882904-6882923 None:intergenic 30.0%
AGAAAACAGAGATGAAAAAC+AGG - chr2_4:6882908-6882927 Msa0311920:CDS 30.0%
GAAAACAGAGATGAAAAACA+GGG - chr2_4:6882909-6882928 Msa0311920:CDS 30.0%
AAAACAGAGATGAAAAACAG+GGG - chr2_4:6882910-6882929 Msa0311920:CDS 30.0%
!!! TCTTGACCAAATTTTCAAGT+GGG - chr2_4:6882962-6882981 Msa0311920:CDS 30.0%
! TTGATGAGTCTTTTGTTGAA+TGG - chr2_4:6883045-6883064 Msa0311920:CDS 30.0%
ATATGAAGATGAAGAAACAG+AGG - chr2_4:6883094-6883113 Msa0311920:CDS 30.0%
ATCTGATTCAGATAGTTCAT+CGG - chr2_4:6883184-6883203 Msa0311920:CDS 30.0%
TTAATTGAGATAGCTCTTGA+TGG - chr2_4:6883287-6883306 Msa0311920:CDS 30.0%
TTAATTCCAACTCAAATCAC+CGG + chr2_4:6883420-6883439 None:intergenic 30.0%
TTTCTTCACTCTTAGATCTC+TGG + chr2_4:6882851-6882870 None:intergenic 35.0%
GAGATCTAAGAGTGAAGAAA+AGG - chr2_4:6882851-6882870 Msa0311920:CDS 35.0%
!! CTCTTGACCAAATTTTCAAG+TGG - chr2_4:6882961-6882980 Msa0311920:CDS 35.0%
TCTAATGAGAATTGTGTTGC+TGG - chr2_4:6883122-6883141 Msa0311920:CDS 35.0%
GAACTATCTGAATCAGATTC+AGG + chr2_4:6883182-6883201 None:intergenic 35.0%
TTTCTTCACTCTTAGATCTC+TGG + chr2_4:6882851-6882870 None:intergenic 35.0%
GAGATCTAAGAGTGAAGAAA+AGG - chr2_4:6882851-6882870 Msa0311920:CDS 35.0%
!! CTCTTGACCAAATTTTCAAG+TGG - chr2_4:6882961-6882980 Msa0311920:CDS 35.0%
TCTAATGAGAATTGTGTTGC+TGG - chr2_4:6883122-6883141 Msa0311920:CDS 35.0%
GAACTATCTGAATCAGATTC+AGG + chr2_4:6883182-6883201 None:intergenic 35.0%
TGAAAAACAGGGGAAAACAG+AGG - chr2_4:6882920-6882939 Msa0311920:CDS 40.0%
TCGAGACCCACTTGAAAATT+TGG + chr2_4:6882971-6882990 None:intergenic 40.0%
TTCATCGGAGAATGAGTTTC+CGG - chr2_4:6883199-6883218 Msa0311920:CDS 40.0%
CACCGGAGAATATGAATTTC+CGG - chr2_4:6883240-6883259 Msa0311920:CDS 40.0%
CACCGGAAATTCATATTCTC+CGG + chr2_4:6883245-6883264 None:intergenic 40.0%
GGAGAATATGAATTTCCGGT+GGG - chr2_4:6883244-6883263 Msa0311920:CDS 40.0%
TGGGATGAAGAAGATAGAGA+AGG - chr2_4:6883263-6883282 Msa0311920:CDS 40.0%
GGGATGAAGAAGATAGAGAA+GGG - chr2_4:6883264-6883283 Msa0311920:CDS 40.0%
!! TAGCTCTTGATGGCTTTAAG+AGG - chr2_4:6883297-6883316 Msa0311920:CDS 40.0%
!! TCTTGATGGCTTTAAGAGGA+AGG - chr2_4:6883301-6883320 Msa0311920:CDS 40.0%
! TGTTTTCCGGTGATTTGAGT+TGG - chr2_4:6883411-6883430 Msa0311920:CDS 40.0%
TGAAAAACAGGGGAAAACAG+AGG - chr2_4:6882920-6882939 Msa0311920:CDS 40.0%
TCGAGACCCACTTGAAAATT+TGG + chr2_4:6882971-6882990 None:intergenic 40.0%
TTCATCGGAGAATGAGTTTC+CGG - chr2_4:6883199-6883218 Msa0311920:CDS 40.0%
CACCGGAGAATATGAATTTC+CGG - chr2_4:6883240-6883259 Msa0311920:CDS 40.0%
CACCGGAAATTCATATTCTC+CGG + chr2_4:6883245-6883264 None:intergenic 40.0%
GGAGAATATGAATTTCCGGT+GGG - chr2_4:6883244-6883263 Msa0311920:CDS 40.0%
TGGGATGAAGAAGATAGAGA+AGG - chr2_4:6883263-6883282 Msa0311920:CDS 40.0%
GGGATGAAGAAGATAGAGAA+GGG - chr2_4:6883264-6883283 Msa0311920:CDS 40.0%
!! TAGCTCTTGATGGCTTTAAG+AGG - chr2_4:6883297-6883316 Msa0311920:CDS 40.0%
!! TCTTGATGGCTTTAAGAGGA+AGG - chr2_4:6883301-6883320 Msa0311920:CDS 40.0%
! TGTTTTCCGGTGATTTGAGT+TGG - chr2_4:6883411-6883430 Msa0311920:CDS 40.0%
GATGAGAATCAAGGTGCAGA+AGG + chr2_4:6882736-6882755 None:intergenic 45.0%
TCACCAGTTCCAATTCTCAC+TGG - chr2_4:6882789-6882808 Msa0311920:CDS 45.0%
ACACCAGTGAGAATTGGAAC+TGG + chr2_4:6882795-6882814 None:intergenic 45.0%
GGGGAAAACAGAGGAAAATG+TGG - chr2_4:6882929-6882948 Msa0311920:CDS 45.0%
CGGAGAATATGAATTTCCGG+TGG - chr2_4:6883243-6883262 Msa0311920:CDS 45.0%
CTCTATCTTCTTCATCCCAC+CGG + chr2_4:6883262-6883281 None:intergenic 45.0%
!! TGGCTTTAAGAGGAAGGCTT+TGG - chr2_4:6883307-6883326 Msa0311920:CDS 45.0%
!! GGCTTTAAGAGGAAGGCTTT+GGG - chr2_4:6883308-6883327 Msa0311920:CDS 45.0%
!! GGCTTTGGGATTTCAGTTTG+AGG - chr2_4:6883322-6883341 Msa0311920:CDS 45.0%
GATGAGAATCAAGGTGCAGA+AGG + chr2_4:6882736-6882755 None:intergenic 45.0%
TCACCAGTTCCAATTCTCAC+TGG - chr2_4:6882789-6882808 Msa0311920:CDS 45.0%
ACACCAGTGAGAATTGGAAC+TGG + chr2_4:6882795-6882814 None:intergenic 45.0%
GGGGAAAACAGAGGAAAATG+TGG - chr2_4:6882929-6882948 Msa0311920:CDS 45.0%
CGGAGAATATGAATTTCCGG+TGG - chr2_4:6883243-6883262 Msa0311920:CDS 45.0%
CTCTATCTTCTTCATCCCAC+CGG + chr2_4:6883262-6883281 None:intergenic 45.0%
!! TGGCTTTAAGAGGAAGGCTT+TGG - chr2_4:6883307-6883326 Msa0311920:CDS 45.0%
!! GGCTTTAAGAGGAAGGCTTT+GGG - chr2_4:6883308-6883327 Msa0311920:CDS 45.0%
!! GGCTTTGGGATTTCAGTTTG+AGG - chr2_4:6883322-6883341 Msa0311920:CDS 45.0%
!! ATGGAACGTGAAAGCGCCGT+TGG - chr2_4:6883064-6883083 Msa0311920:CDS 55.0%
GGAGAATGAGTTTCCGGCGA+TGG - chr2_4:6883205-6883224 Msa0311920:CDS 55.0%
AGTTTCCGGCGATGGAGAAC+TGG - chr2_4:6883213-6883232 Msa0311920:CDS 55.0%
GTTTCCGGCGATGGAGAACT+GGG - chr2_4:6883214-6883233 Msa0311920:CDS 55.0%
GATGGAGAACTGGGACTCAC+CGG - chr2_4:6883223-6883242 Msa0311920:CDS 55.0%
ACATTTCTCCGACGAAGCGC+AGG - chr2_4:6883366-6883385 Msa0311920:CDS 55.0%
CATTTCTCCGACGAAGCGCA+GGG - chr2_4:6883367-6883386 Msa0311920:CDS 55.0%
GAAAACTCCCTGCGCTTCGT+CGG + chr2_4:6883377-6883396 None:intergenic 55.0%
! ACGAAGCGCAGGGAGTTTTC+CGG - chr2_4:6883377-6883396 Msa0311920:CDS 55.0%
!! ATGGAACGTGAAAGCGCCGT+TGG - chr2_4:6883064-6883083 Msa0311920:CDS 55.0%
GGAGAATGAGTTTCCGGCGA+TGG - chr2_4:6883205-6883224 Msa0311920:CDS 55.0%
AGTTTCCGGCGATGGAGAAC+TGG - chr2_4:6883213-6883232 Msa0311920:CDS 55.0%
GTTTCCGGCGATGGAGAACT+GGG - chr2_4:6883214-6883233 Msa0311920:CDS 55.0%
GATGGAGAACTGGGACTCAC+CGG - chr2_4:6883223-6883242 Msa0311920:CDS 55.0%
ACATTTCTCCGACGAAGCGC+AGG - chr2_4:6883366-6883385 Msa0311920:CDS 55.0%
CATTTCTCCGACGAAGCGCA+GGG - chr2_4:6883367-6883386 Msa0311920:CDS 55.0%
GAAAACTCCCTGCGCTTCGT+CGG + chr2_4:6883377-6883396 None:intergenic 55.0%
! ACGAAGCGCAGGGAGTTTTC+CGG - chr2_4:6883377-6883396 Msa0311920:CDS 55.0%
GAGTCCCAGTTCTCCATCGC+CGG + chr2_4:6883221-6883240 None:intergenic 60.0%
GGAAAACACCTCCTCCTCGC+CGG + chr2_4:6883399-6883418 None:intergenic 60.0%
!!! GGCGAGGAGGAGGTGTTTTC+CGG - chr2_4:6883398-6883417 Msa0311920:CDS 60.0%
GAGTCCCAGTTCTCCATCGC+CGG + chr2_4:6883221-6883240 None:intergenic 60.0%
GGAAAACACCTCCTCCTCGC+CGG + chr2_4:6883399-6883418 None:intergenic 60.0%
!!! GGCGAGGAGGAGGTGTTTTC+CGG - chr2_4:6883398-6883417 Msa0311920:CDS 60.0%
! CAGGGAGTTTTCCGGCGAGG+AGG - chr2_4:6883385-6883404 Msa0311920:CDS 65.0%
! GGAGTTTTCCGGCGAGGAGG+AGG - chr2_4:6883388-6883407 Msa0311920:CDS 65.0%
! CAGGGAGTTTTCCGGCGAGG+AGG - chr2_4:6883385-6883404 Msa0311920:CDS 65.0%
! GGAGTTTTCCGGCGAGGAGG+AGG - chr2_4:6883388-6883407 Msa0311920:CDS 65.0%
! GCGCAGGGAGTTTTCCGGCG+AGG - chr2_4:6883382-6883401 Msa0311920:CDS 70.0%
! GCGCAGGGAGTTTTCCGGCG+AGG - chr2_4:6883382-6883401 Msa0311920:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 6882657 6883439 6882657 ID=Msa0311920;Name=Msa0311920
chr2_4 mRNA 6882657 6883439 6882657 ID=Msa0311920-mRNA-1;Parent=Msa0311920;Name=Msa0311920-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|260
chr2_4 exon 6882657 6883439 6882657 ID=Msa0311920-mRNA-1:exon:2628;Parent=Msa0311920-mRNA-1
chr2_4 CDS 6882657 6883439 6882657 ID=Msa0311920-mRNA-1:cds;Parent=Msa0311920-mRNA-1
Gene Sequence

>Msa0311920

ATGAACTTGTTATGCAAAGAAACTTTTAACTTTCTCTCAATTGTTTCTTCAAATCCTCTGTTTTCATGCATTATTACCTTCTGCACCTTGATTCTCATCTATTTTCCTTATTCATTCTGCAAAATAGTTTTCTCACCAGTTCCAATTCTCACTGGTGTTATATTGATTTTTGTACTTCGTTTTGGTGCAATCCAGAGATCTAAGAGTGAAGAAAAGGAAAAATTTGGTGAATTAGAATCAGTTACCAATGAAGAAAACAGAGATGAAAAACAGGGGAAAACAGAGGAAAATGTGGAAAATGATTCTCTTGACCAAATTTTCAAGTGGGTCTCGAGAAATTCCGAAGAAAAGTTGAAATCTAAAATGGGTTTTGAATCAAATTCGTTTCTTGATGAGTCTTTTGTTGAATGGAACGTGAAAGCGCCGTTGGAAGTTATATATGAAGATGAAGAAACAGAGGATATTTCTAATGAGAATTGTGTTGCTGGAATTTTGAGACACTCTTCATTGTCACGTTATTATCCTGAATCTGATTCAGATAGTTCATCGGAGAATGAGTTTCCGGCGATGGAGAACTGGGACTCACCGGAGAATATGAATTTCCGGTGGGATGAAGAAGATAGAGAAGGGTTAATTGAGATAGCTCTTGATGGCTTTAAGAGGAAGGCTTTGGGATTTCAGTTTGAGGAAGAGAATATGATTGAGATTGACATTTCTCCGACGAAGCGCAGGGAGTTTTCCGGCGAGGAGGAGGTGTTTTCCGGTGATTTGAGTTGGAATTAA

Protein sequence

>Msa0311920

MNLLCKETFNFLSIVSSNPLFSCIITFCTLILIYFPYSFCKIVFSPVPILTGVILIFVLRFGAIQRSKSEEKEKFGELESVTNEENRDEKQGKTEENVENDSLDQIFKWVSRNSEEKLKSKMGFESNSFLDESFVEWNVKAPLEVIYEDEETEDISNENCVAGILRHSSLSRYYPESDSDSSSENEFPAMENWDSPENMNFRWDEEDREGLIEIALDGFKRKALGFQFEEENMIEIDISPTKRREFSGEEEVFSGDLSWN*