AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0140910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0140910 MtrunA17_Chr2g0310011 90.160 376 5 3 1 372 1 348 0.0 679
Msa0140910 MtrunA17_Chr8g0356501 63.144 388 84 17 1 372 1 345 3.55e-144 412
Msa0140910 MtrunA17_Chr6g0461641 56.098 164 56 2 28 175 35 198 1.87e-52 179
Msa0140910 MtrunA17_Chr2g0321461 55.882 170 53 4 25 173 35 203 1.15e-51 176
Msa0140910 MtrunA17_Chr5g0417521 55.414 157 58 2 28 172 8 164 1.44e-49 170
Msa0140910 MtrunA17_Chr4g0042451 56.410 156 53 3 28 170 10 163 1.46e-49 169
Msa0140910 MtrunA17_Chr5g0409731 53.374 163 58 5 22 168 10 170 3.39e-49 167
Msa0140910 MtrunA17_Chr1g0197171 51.977 177 73 4 14 180 23 197 1.70e-47 163
Msa0140910 MtrunA17_Chr2g0290991 50.877 171 69 4 23 180 27 195 3.33e-44 155
Msa0140910 MtrunA17_Chr7g0254981 54.605 152 54 5 35 178 50 194 9.59e-44 152
Msa0140910 MtrunA17_Chr2g0291021 57.971 138 55 2 35 170 49 185 1.54e-42 151
Msa0140910 MtrunA17_Chr7g0261911 50.370 135 55 3 35 163 262 390 7.23e-36 136
Msa0140910 MtrunA17_Chr7g0274471 43.038 158 76 3 35 186 218 367 3.00e-34 131
Msa0140910 MtrunA17_Chr1g0190301 44.218 147 66 3 25 163 165 303 4.87e-34 129
Msa0140910 MtrunA17_Chr1g0175091 44.526 137 74 1 31 167 254 388 3.40e-31 122
Msa0140910 MtrunA17_Chr7g0258551 37.572 173 102 3 31 198 208 379 3.82e-29 116
Msa0140910 MtrunA17_Chr7g0242521 47.287 129 64 2 35 160 13 140 4.60e-28 110
Msa0140910 MtrunA17_Chr1g0175071 38.961 154 83 3 25 173 167 314 1.26e-23 99.8
Msa0140910 MtrunA17_Chr3g0095611 61.111 54 21 0 35 88 19 72 6.36e-16 75.9
Msa0140910 MtrunA17_Chr6g0479431 56.667 60 26 0 36 95 203 262 2.06e-15 77.0
Msa0140910 MtrunA17_Chr7g0243081 44.444 81 45 0 11 91 47 127 2.56e-15 76.3
Msa0140910 MtrunA17_Chr2g0288601 61.111 54 21 0 36 89 72 125 2.82e-15 72.0
Msa0140910 MtrunA17_Chr1g0210911 56.452 62 22 1 36 92 23 84 3.68e-15 75.9
Msa0140910 MtrunA17_Chr1g0154261 46.667 75 40 0 36 110 127 201 4.78e-15 75.1
Msa0140910 MtrunA17_Chr1g0162931 38.261 115 52 2 36 135 19 129 4.83e-15 74.7
Msa0140910 MtrunA17_Chr8g0366281 58.929 56 23 0 36 91 174 229 5.98e-15 75.5
Msa0140910 MtrunA17_Chr3g0109981 50.704 71 35 0 36 106 275 345 7.85e-15 75.5
Msa0140910 MtrunA17_Chr5g0410741 58.929 56 23 0 36 91 220 275 8.38e-15 75.1
Msa0140910 MtrunA17_Chr1g0175081 49.091 55 28 0 37 91 24 78 9.88e-15 69.7
Msa0140910 MtrunA17_Chr8g0379121 53.571 56 26 0 36 91 112 167 3.75e-14 73.6
Msa0140910 MtrunA17_Chr2g0320231 55.357 56 25 0 36 91 98 153 6.55e-14 72.4
Msa0140910 MtrunA17_Chr7g0258531 35.821 134 67 4 35 158 213 337 6.88e-14 72.0
Msa0140910 MtrunA17_Chr1g0161431 61.818 55 20 1 35 89 200 253 2.46e-13 71.2
Msa0140910 MtrunA17_Chr2g0283951 58.000 50 21 0 36 85 90 139 6.73e-13 69.3
Msa0140910 MtrunA17_Chr4g0031961 52.174 69 31 2 22 89 117 184 6.86e-13 68.9
Msa0140910 MtrunA17_Chr1g0198601 49.315 73 37 0 35 107 211 283 6.98e-13 69.3
Msa0140910 MtrunA17_Chr4g0067981 57.627 59 24 1 31 89 211 268 7.24e-13 70.1
Msa0140910 MtrunA17_Chr5g0402631 51.316 76 34 1 35 110 214 286 1.14e-12 68.6
Msa0140910 MtrunA17_Chr2g0294911 53.333 60 27 1 30 89 206 264 1.15e-12 69.3
Msa0140910 MtrunA17_Chr7g0255711 60.000 55 21 1 35 89 91 144 1.74e-12 67.4
Msa0140910 MtrunA17_Chr4g0009901 52.542 59 27 1 31 89 164 221 9.80e-12 65.1
Msa0140910 MtrunA17_Chr5g0422621 58.182 55 23 0 35 89 198 252 1.31e-11 65.5
Msa0140910 MtrunA17_Chr1g0149841 52.542 59 27 1 31 89 187 244 4.02e-11 64.3
Msa0140910 MtrunA17_Chr4g0009981 53.448 58 26 1 31 88 112 168 4.96e-11 62.8
Msa0140910 MtrunA17_Chr4g0045361 55.172 58 26 0 32 89 271 328 5.02e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0140910 AT1G79430.2 56.122 392 115 16 4 372 1 358 4.91e-121 354
Msa0140910 AT1G79430.1 50.459 327 107 15 67 372 1 293 6.32e-78 242
Msa0140910 AT3G12730.1 70.395 152 37 3 24 167 12 163 8.32e-68 214
Msa0140910 AT4G13640.1 66.667 165 41 3 25 176 27 190 1.39e-65 210
Msa0140910 AT3G24120.1 68.987 158 35 3 24 168 30 186 8.58e-65 208
Msa0140910 AT4G13640.2 65.476 168 41 4 25 176 27 193 1.80e-63 205
Msa0140910 AT3G24120.2 67.702 161 35 4 24 168 30 189 1.14e-62 202
Msa0140910 AT3G04030.3 60.355 169 50 4 24 176 34 201 5.30e-57 191
Msa0140910 AT5G18240.1 60.714 168 51 3 24 176 34 201 3.29e-56 189
Msa0140910 AT5G18240.4 60.714 168 51 3 24 176 34 201 3.29e-56 189
Msa0140910 AT3G04030.2 59.172 169 51 5 24 176 34 200 6.06e-54 183
Msa0140910 AT5G18240.5 59.524 168 51 4 24 176 34 199 6.58e-53 180
Msa0140910 AT3G04030.1 57.396 169 49 5 24 176 34 195 1.45e-50 174
Msa0140910 AT5G18240.2 57.738 168 50 4 24 176 34 195 1.14e-49 172
Msa0140910 AT5G18240.3 57.738 168 50 4 24 176 34 195 1.14e-49 172
Msa0140910 AT1G69580.1 46.341 205 81 6 27 212 26 220 3.04e-49 169
Msa0140910 AT1G69580.2 46.117 206 81 6 27 212 26 221 4.37e-49 169
Msa0140910 AT5G45580.2 46.914 162 68 2 25 168 13 174 2.43e-43 152
Msa0140910 AT2G01060.1 56.934 137 57 2 35 170 15 150 6.66e-43 151
Msa0140910 AT5G45580.1 47.273 165 65 3 29 171 1 165 2.94e-42 149
Msa0140910 AT3G12730.2 62.385 109 33 3 67 167 1 109 3.42e-37 133
Msa0140910 AT3G13040.3 44.720 161 75 4 21 167 218 378 1.94e-33 129
Msa0140910 AT3G13040.1 44.720 161 75 4 21 167 218 378 1.94e-33 129
Msa0140910 AT3G13040.2 44.720 161 75 4 21 167 218 378 1.94e-33 129
Msa0140910 AT5G29000.1 41.520 171 82 5 35 196 188 349 1.35e-32 125
Msa0140910 AT5G29000.3 41.520 171 82 5 35 196 188 349 1.35e-32 125
Msa0140910 AT4G28610.1 44.928 138 59 3 35 163 225 354 2.64e-32 125
Msa0140910 AT5G29000.2 41.520 171 82 5 35 196 231 392 3.07e-32 125
Msa0140910 AT5G06800.1 47.287 129 67 1 35 163 192 319 1.92e-31 122
Msa0140910 AT3G04450.4 41.935 155 73 4 35 180 198 344 3.77e-31 122
Msa0140910 AT3G04450.2 41.935 155 73 4 35 180 198 344 3.77e-31 122
Msa0140910 AT5G06800.2 45.522 134 67 2 35 163 192 324 4.49e-31 122
Msa0140910 AT3G04450.1 41.935 155 73 4 35 180 238 384 6.55e-31 122
Msa0140910 AT3G04030.5 54.762 126 40 4 67 176 1 125 6.35e-30 117
Msa0140910 AT2G20400.1 42.177 147 79 3 35 178 231 374 1.13e-26 110
Msa0140910 AT2G20400.2 41.497 147 81 2 35 178 231 375 3.01e-26 108
Msa0140910 AT3G04030.4 50.794 126 39 5 67 176 1 119 9.94e-24 100
Msa0140910 AT5G18240.6 50.400 125 41 4 67 176 1 119 4.38e-23 98.6
Msa0140910 AT2G06020.1 59.375 64 26 0 28 91 79 142 8.32e-21 92.0
Msa0140910 AT2G01060.2 49.020 102 50 2 70 170 1 101 8.71e-21 90.5
Msa0140910 AT5G06800.3 59.649 57 23 0 35 91 192 248 3.17e-18 84.3
Msa0140910 AT5G29000.4 57.895 57 24 0 35 91 231 287 2.84e-17 82.0
Msa0140910 AT3G04450.3 56.140 57 25 0 35 91 238 294 3.04e-17 82.0
Msa0140910 AT1G14600.1 50.685 73 31 1 21 88 4 76 2.07e-16 78.6
Msa0140910 AT2G40260.1 60.714 56 22 0 36 91 83 138 7.94e-16 79.0
Msa0140910 AT4G04605.1 58.491 53 22 0 36 88 15 67 1.01e-15 75.9
Msa0140910 AT2G42660.1 58.929 56 23 0 36 91 51 106 1.10e-15 76.6
Msa0140910 AT2G02060.2 57.627 59 25 0 34 92 28 86 2.45e-15 75.5
Msa0140910 AT2G02060.1 57.627 59 25 0 34 92 28 86 2.45e-15 75.5
Msa0140910 AT1G32240.2 56.667 60 26 0 36 95 214 273 6.42e-15 75.9
Msa0140910 AT5G16560.1 56.667 60 26 0 36 95 220 279 6.45e-15 75.9
Msa0140910 AT1G32240.1 56.667 60 26 0 36 95 214 273 7.98e-15 75.5
Msa0140910 AT2G38300.1 57.143 56 24 0 36 91 55 110 1.56e-14 74.3
Msa0140910 AT5G42630.2 57.143 56 24 0 36 91 106 161 1.74e-14 72.4
Msa0140910 AT5G42630.1 57.143 56 24 0 36 91 106 161 2.37e-14 73.2
Msa0140910 AT4G17695.1 53.333 60 28 0 36 95 165 224 5.18e-14 72.8
Msa0140910 AT3G46640.2 54.286 70 28 2 23 89 129 197 2.11e-13 70.9
Msa0140910 AT3G46640.1 54.286 70 28 2 23 89 129 197 2.11e-13 70.9
Msa0140910 AT3G46640.3 54.286 70 28 2 23 89 129 197 2.76e-13 70.5
Msa0140910 AT5G59570.2 52.857 70 29 2 23 89 126 194 4.18e-13 69.7
Msa0140910 AT5G59570.1 52.857 70 29 2 23 89 126 194 4.18e-13 69.7
Msa0140910 AT4G04580.1 49.123 57 29 0 36 92 15 71 6.80e-13 66.6
Msa0140910 AT5G05090.1 60.000 55 21 1 35 89 81 134 7.74e-13 68.6
Msa0140910 AT3G10760.1 60.000 55 21 1 35 89 105 158 9.84e-13 68.9
Msa0140910 AT4G37180.1 55.172 58 26 0 37 94 212 269 1.37e-12 68.6
Msa0140910 AT4G37180.2 55.172 58 26 0 37 94 219 276 1.57e-12 68.6
Msa0140910 AT2G40970.1 60.000 55 21 1 35 89 105 158 2.27e-12 67.0
Msa0140910 AT1G49560.1 48.235 85 40 1 35 119 192 272 6.59e-12 66.6
Msa0140910 AT3G16857.3 56.364 55 23 1 35 89 167 220 6.69e-12 67.4
Msa0140910 AT3G16857.2 56.364 55 23 1 35 89 237 290 7.67e-12 67.4
Msa0140910 AT3G16857.1 56.364 55 23 1 35 89 237 290 7.69e-12 67.4
Msa0140910 AT5G58080.1 55.172 58 25 1 32 89 191 247 8.33e-12 67.0
Msa0140910 AT3G25790.1 60.000 50 20 0 40 89 201 250 8.39e-12 66.2
Msa0140910 AT2G01760.3 54.545 55 24 1 35 89 135 188 1.05e-11 65.9
Msa0140910 AT2G01760.1 54.545 55 24 1 35 89 200 253 1.92e-11 65.5
Msa0140910 AT2G01760.2 54.545 55 24 1 35 89 228 281 2.86e-11 65.1
Msa0140910 AT5G44190.1 55.556 54 23 1 35 88 147 199 4.85e-11 64.3
Msa0140910 AT5G44190.2 55.556 54 23 1 35 88 147 199 5.91e-11 63.5
Msa0140910 AT1G13300.1 54.545 55 25 0 35 89 181 235 8.72e-11 63.2

Find 113 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGGACTATTGAAAGAATT+TGG 0.175321 1_3:-75562990 Msa0140910:CDS
GATTTAAGGCTTCAAGATTT+AGG 0.193912 1_3:-75562750 Msa0140910:CDS
TGCTACAACTAATAACTTAA+AGG 0.195092 1_3:-75563045 Msa0140910:CDS
CTTTGTTGTGAAACTCTCTT+AGG 0.204487 1_3:-75562390 Msa0140910:three_prime_UTR
TAATGTGGAGTGATGATTTA+AGG 0.215191 1_3:-75562764 Msa0140910:CDS
AAATCATCACTCCACATTAA+TGG 0.218919 1_3:+75562768 None:intergenic
TTTCTATTACATAGAAATTT+AGG 0.227246 1_3:-75563942 Msa0140910:intron
TTCATAGTTGAAGGCTTCTT+TGG 0.249952 1_3:+75564585 None:intergenic
AACAAATGCGGATGCAAAAT+AGG 0.252816 1_3:+75564657 None:intergenic
AACTTAAAGGGCGTTGTTCT+TGG 0.261188 1_3:-75563032 Msa0140910:CDS
CCACTATAAGGGTTAGTGTT+AGG 0.267537 1_3:+75562804 None:intergenic
GCATATATGCATGATGATCT+AGG 0.285163 1_3:+75562868 None:intergenic
ATTTAAGGCTTCAAGATTTA+GGG 0.292742 1_3:-75562749 Msa0140910:CDS
AATCATCACTCCACATTAAT+GGG 0.299412 1_3:+75562769 None:intergenic
TAAAACTATCATGAGGGTTA+TGG 0.306320 1_3:-75564306 Msa0140910:CDS
GAAATACATGCAAAGTATAT+TGG 0.312337 1_3:-75563108 Msa0140910:CDS
ATTCAATGATCACTCAATTA+AGG 0.327183 1_3:-75563896 Msa0140910:CDS
GTCCAACGAAGACGAGGTTT+TGG 0.340512 1_3:+75564504 None:intergenic
TCAAATTGCACCACCATCAT+TGG 0.344106 1_3:-75562682 Msa0140910:CDS
CCATCTCAAGAGCCATCTTC+AGG 0.344761 1_3:-75564261 Msa0140910:intron
TGAAGAGATGGCCCTCTTCT+TGG 0.346647 1_3:+75562543 None:intergenic
TATGATTAGTACTGGTACTA+TGG 0.347038 1_3:-75562502 Msa0140910:CDS
CACTATAAGGGTTAGTGTTA+GGG 0.355596 1_3:+75562805 None:intergenic
AAAGCTTATCAGACACTTGC+AGG 0.356175 1_3:-75563083 Msa0140910:CDS
GCTTCCTCTTCGGCTATGAT+TGG 0.358723 1_3:-75563718 Msa0140910:CDS
ACCTCATGGAATGGCTGAAA+TGG 0.378868 1_3:-75563009 Msa0140910:CDS
GAGCTTCAATTCGTAGTTGA+AGG 0.381017 1_3:+75563135 None:intergenic
AAAACTATCATGAGGGTTAT+GGG 0.387308 1_3:-75564305 Msa0140910:CDS
AAAATTTCCCCTTGAAGTAA+AGG 0.387443 1_3:+75562615 None:intergenic
ACTAACCCTTATAGTGGAAA+TGG 0.388772 1_3:-75562798 Msa0140910:CDS
CAGCATCAACAAAACGTTCA+TGG 0.392478 1_3:+75564472 None:intergenic
CAAGGTAGAGGCTCACCATT+TGG 0.401119 1_3:-75562477 Msa0140910:CDS
GATACAAAGCCTTTACTTCA+AGG 0.414154 1_3:-75562624 Msa0140910:CDS
GAAATCATCATGTTCTTATG+AGG 0.415377 1_3:-75562349 Msa0140910:three_prime_UTR
TCATGTGAATTCATAGTTGA+AGG 0.417635 1_3:+75564576 None:intergenic
ACAAATGCGGATGCAAAATA+GGG 0.425308 1_3:+75564658 None:intergenic
CCTAACACTAACCCTTATAG+TGG 0.433462 1_3:-75562804 Msa0140910:CDS
GGCGTTGTTCTTGGACCTCA+TGG 0.435582 1_3:-75563023 Msa0140910:CDS
ATGAGGGTTATGGGTGTAAA+GGG 0.439033 1_3:-75564296 Msa0140910:CDS
GTTGATGCTGTTACTCAGCT+TGG 0.442902 1_3:-75564459 Msa0140910:CDS
ATGCAAAATAGGGAAGAATA+TGG 0.444532 1_3:+75564668 None:intergenic
CATGAGGGTTATGGGTGTAA+AGG 0.448006 1_3:-75564297 Msa0140910:CDS
AGGGACAGCTTCATCATGCT+TGG 0.454830 1_3:-75562730 Msa0140910:CDS
TTTGTTGTGAAACTCTCTTA+GGG 0.456419 1_3:-75562389 Msa0140910:three_prime_UTR
TTTGTATTCAAACCTGAAGA+TGG 0.457979 1_3:+75564249 None:intergenic
TAAGGGTTAGTGTTAGGGTT+AGG 0.458671 1_3:+75562810 None:intergenic
ACGGAATACTGCTTCCTCTT+CGG 0.462582 1_3:-75563728 Msa0140910:CDS
GCTACAACTAATAACTTAAA+GGG 0.463497 1_3:-75563044 Msa0140910:CDS
CATATATGCATGATGATCTA+GGG 0.467621 1_3:+75562869 None:intergenic
TGAAAAGATGTAAAACTAAG+AGG 0.472361 1_3:+75562963 None:intergenic
GTGATCATTGAATTCCTTGT+GGG 0.478768 1_3:+75563906 None:intergenic
ACTTAAGCCTTGGCAAGAAA+AGG 0.483728 1_3:-75562833 Msa0140910:CDS
ATAGTACCAGTACTAATCAT+AGG 0.485418 1_3:+75562504 None:intergenic
CACCACCACCGCCTCCGAAA+AGG 0.487952 1_3:+75562931 None:intergenic
ATAGTTGAAGGCTTCTTTGG+AGG 0.491992 1_3:+75564588 None:intergenic
ATAACTAATTCTCATCCAAA+TGG 0.494435 1_3:+75562462 None:intergenic
ATTACATAGAAATTTAGGCT+TGG 0.496295 1_3:-75563937 Msa0140910:intron
GATAGACCCATGTGTGTTCA+AGG 0.496295 1_3:-75564555 Msa0140910:CDS
CCTCATGGAATGGCTGAAAT+GGG 0.498758 1_3:-75563008 Msa0140910:CDS
ATACAAAGCCTTTACTTCAA+GGG 0.499005 1_3:-75562623 Msa0140910:CDS
TAGTTGAAGGCTTCTTTGGA+GGG 0.504455 1_3:+75564589 None:intergenic
GAAGAGAAAATGGAAGTGAA+TGG 0.505470 1_3:+75564703 None:intergenic
TGAATTTGATCACCTTTGAA+AGG 0.506520 1_3:+75562702 None:intergenic
CGTTAGAATAATTAAAACGT+TGG 0.509154 1_3:+75562305 None:intergenic
ATGGCTCTTGAGATGGTAAA+GGG 0.513730 1_3:+75564268 None:intergenic
CGGTATTCTCTTCACAGAGA+TGG 0.514178 1_3:-75564622 Msa0140910:exon
ATGAGTCCTATGATTAGTAC+TGG 0.525466 1_3:-75562510 Msa0140910:CDS
ACCCAAAACCTCGTCTTCGT+TGG 0.527604 1_3:-75564506 Msa0140910:CDS
GATGGCCCTCTTCTTGGTGA+GGG 0.531501 1_3:+75562549 None:intergenic
TACAAAGCCTTTACTTCAAG+GGG 0.533890 1_3:-75562622 Msa0140910:CDS
TCACTTCCTCTATCCAATGA+TGG 0.535417 1_3:+75562669 None:intergenic
GATGGCTCTTGAGATGGTAA+AGG 0.536000 1_3:+75564267 None:intergenic
TGTTCTTGGACCTCATGGAA+TGG 0.536570 1_3:-75563018 Msa0140910:CDS
AGATGGCCCTCTTCTTGGTG+AGG 0.537995 1_3:+75562548 None:intergenic
AAACGTTGGTTTGCCATGCA+AGG 0.538717 1_3:+75562319 None:intergenic
GCACCACCATCATTGGATAG+AGG 0.539525 1_3:-75562675 Msa0140910:CDS
ATCAATGAGAACTTAAGCCT+TGG 0.540269 1_3:-75562843 Msa0140910:CDS
ATGTGTGTTCAAGGTGACTC+AGG 0.543370 1_3:-75564546 Msa0140910:CDS
GGTACTATGGCACAAGGTAG+AGG 0.548888 1_3:-75562489 Msa0140910:CDS
CCTGAAGATGGCTCTTGAGA+TGG 0.549039 1_3:+75564261 None:intergenic
TGCTTGGATGATCCTTTCAA+AGG 0.557843 1_3:-75562714 Msa0140910:CDS
GATGCTGTTACTCAGCTTGG+AGG 0.559349 1_3:-75564456 Msa0140910:CDS
CAACTACGAATTGAAGCTCA+AGG 0.565636 1_3:-75563131 Msa0140910:CDS
ATGCATACTCATTCATGTTG+CGG 0.570245 1_3:+75563695 None:intergenic
ATTCTTTCAGCATGAAGAGA+TGG 0.571803 1_3:+75562531 None:intergenic
GACACTTGCAGGTGAAAACA+TGG 0.571973 1_3:-75563072 Msa0140910:CDS
CTTTGCTTTATACTTTGAGA+AGG 0.579123 1_3:-75564736 Msa0140910:five_prime_UTR
CTTCAGCTTTAGAACTGCAA+CGG 0.582298 1_3:-75563747 Msa0140910:CDS
ACTTGACCTTCAACAAAACA+TGG 0.583014 1_3:-75562901 Msa0140910:CDS
ATGAGGTATAATGCCTTGCA+TGG 0.587240 1_3:-75562332 Msa0140910:three_prime_UTR
TGAGAATGCTCACATTAACT+AGG 0.597203 1_3:+75562254 None:intergenic
TGAGTCACCTTGAACACACA+TGG 0.598179 1_3:+75564548 None:intergenic
GAAAGACCCTCACCAAGAAG+AGG 0.599610 1_3:-75562555 Msa0140910:CDS
AATGATCACTCAATTAAGGA+TGG 0.602702 1_3:-75563892 Msa0140910:intron
AAGTATAAAGCAAAGTGAGA+AGG 0.606762 1_3:+75564744 None:intergenic
CTAAGAGAGTTTCACAACAA+AGG 0.608999 1_3:+75562391 None:intergenic
AAAGACCCTCACCAAGAAGA+GGG 0.613710 1_3:-75562554 Msa0140910:CDS
GCTTGGAAAGCAACCCCACA+AGG 0.615422 1_3:-75563920 Msa0140910:CDS
CCACTCCTAAAACTATCATG+AGG 0.619863 1_3:-75564313 Msa0140910:CDS
TGATCATTGAATTCCTTGTG+GGG 0.623211 1_3:+75563907 None:intergenic
AACTACGAATTGAAGCTCAA+GGG 0.632870 1_3:-75563130 Msa0140910:CDS
CACTCCTAAAACTATCATGA+GGG 0.634268 1_3:-75564312 Msa0140910:CDS
CATTTGTTCTTGAATCTCTG+CGG 0.639752 1_3:-75564642 Msa0140910:five_prime_UTR
AGTGATCATTGAATTCCTTG+TGG 0.639862 1_3:+75563905 None:intergenic
TCAACAGTCCAACGAAGACG+AGG 0.644546 1_3:+75564498 None:intergenic
TGTAATCAGGATGCAAATTG+AGG 0.645996 1_3:-75563479 Msa0140910:intron
CCCATTTCAGCCATTCCATG+AGG 0.656331 1_3:+75563008 None:intergenic
CTTCCTCTATCCAATGATGG+TGG 0.659128 1_3:+75562672 None:intergenic
GCGGCCAATCATAGCCGAAG+AGG 0.663510 1_3:+75563714 None:intergenic
AAGACTACATGAGCAACTTG+AGG 0.669000 1_3:-75563449 Msa0140910:intron
CAGAGATTCAAGAACAAATG+CGG 0.674681 1_3:+75564645 None:intergenic
GAGTCACCTTGAACACACAT+GGG 0.682407 1_3:+75564549 None:intergenic
AGTACTGGTACTATGGCACA+AGG 0.707110 1_3:-75562495 Msa0140910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTTGTTATTTTTATATT+TGG - chr1_3:75562591-75562610 Msa0140910:CDS 10.0%
!!! TTTTAATTTTTTGAACTTTA+TGG - chr1_3:75563146-75563165 Msa0140910:CDS 10.0%
!!! TGATTTGAATCTTTTTTTTA+TGG - chr1_3:75562935-75562954 Msa0140910:CDS 15.0%
!!! TTTTTTATGGTTAATTTTTC+AGG - chr1_3:75562948-75562967 Msa0140910:CDS 15.0%
!! GGAAAAACAATAAAAAAAAA+AGG + chr1_3:75563003-75563022 None:intergenic 15.0%
!! TTTCTATTACATAGAAATTT+AGG - chr1_3:75563030-75563049 Msa0140910:CDS 15.0%
!! TTAAAATAAGAGATGTATAA+TGG + chr1_3:75563318-75563337 None:intergenic 15.0%
!! ATACTAATTATGTAACAAAT+TGG + chr1_3:75563762-75563781 None:intergenic 15.0%
!!! TACATAATTAGTATTTTCAA+TGG - chr1_3:75563768-75563787 Msa0140910:intron 15.0%
!! CATTACTAAGATATTTATGA+TGG - chr1_3:75562616-75562635 Msa0140910:CDS 20.0%
!! TTCTTTACAACACTTTAATA+TGG - chr1_3:75562783-75562802 Msa0140910:CDS 20.0%
!!! CAGCAAAATAATTTTTAACT+GGG + chr1_3:75562893-75562912 None:intergenic 20.0%
!! ATAATACATAATCATGTGTA+AGG + chr1_3:75563173-75563192 None:intergenic 20.0%
!! ATTGACAAGATCTTTATATA+TGG - chr1_3:75563199-75563218 Msa0140910:intron 20.0%
!!! TTTGTTTTGAATCTTTTTGT+TGG - chr1_3:75563401-75563420 Msa0140910:intron 20.0%
!!! TTGTTTTGAATCTTTTTGTT+GGG - chr1_3:75563402-75563421 Msa0140910:intron 20.0%
!! TTTATAAGTGCACATATATT+TGG - chr1_3:75563583-75563602 Msa0140910:intron 20.0%
!! TGAATTTAGTTGTATATTTC+AGG - chr1_3:75563804-75563823 Msa0140910:intron 20.0%
! TTTGAGAAGAGAAAGAAAAA+AGG + chr1_3:75562567-75562586 None:intergenic 25.0%
!!! TGGTTTCTTAAATTTTTCAG+AGG - chr1_3:75562636-75562655 Msa0140910:CDS 25.0%
!! GCAGCAAAATAATTTTTAAC+TGG + chr1_3:75562894-75562913 None:intergenic 25.0%
!!! TAAAAATTATTTTGCTGCAC+CGG - chr1_3:75562896-75562915 Msa0140910:CDS 25.0%
! CTATGTAATAGAAACAACAA+AGG + chr1_3:75563024-75563043 None:intergenic 25.0%
! ATTACATAGAAATTTAGGCT+TGG - chr1_3:75563035-75563054 Msa0140910:CDS 25.0%
! ATTCAATGATCACTCAATTA+AGG - chr1_3:75563076-75563095 Msa0140910:CDS 25.0%
!!! TGTTTTGAATCTTTTTGTTG+GGG - chr1_3:75563403-75563422 Msa0140910:intron 25.0%
! AATTGTATATATAGTGCATC+CGG - chr1_3:75563725-75563744 Msa0140910:CDS 25.0%
! GAAATACATGCAAAGTATAT+TGG - chr1_3:75563864-75563883 Msa0140910:intron 25.0%
! TGCTACAACTAATAACTTAA+AGG - chr1_3:75563927-75563946 Msa0140910:CDS 25.0%
! GCTACAACTAATAACTTAAA+GGG - chr1_3:75563928-75563947 Msa0140910:CDS 25.0%
! TGAAAAGATGTAAAACTAAG+AGG + chr1_3:75564012-75564031 None:intergenic 25.0%
!! TTTTTCCATTTCCACTATAA+GGG + chr1_3:75564182-75564201 None:intergenic 25.0%
!!! TTTTTTCCATTTCCACTATA+AGG + chr1_3:75564183-75564202 None:intergenic 25.0%
!! ATTTAAGGCTTCAAGATTTA+GGG - chr1_3:75564223-75564242 Msa0140910:intron 25.0%
! ATAACTAATTCTCATCCAAA+TGG + chr1_3:75564513-75564532 None:intergenic 25.0%
! CGTTAGAATAATTAAAACGT+TGG + chr1_3:75564670-75564689 None:intergenic 25.0%
!!! TCTCACTTGTATGTTTTTTT+AGG - chr1_3:75564736-75564755 Msa0140910:five_prime_UTR 25.0%
AAGTATAAAGCAAAGTGAGA+AGG + chr1_3:75562231-75562250 None:intergenic 30.0%
! CTTTGCTTTATACTTTGAGA+AGG - chr1_3:75562236-75562255 Msa0140910:three_prime_UTR 30.0%
!! CGTTTTTGAAGAAGAGAAAA+TGG + chr1_3:75562282-75562301 None:intergenic 30.0%
ATGCAAAATAGGGAAGAATA+TGG + chr1_3:75562307-75562326 None:intergenic 30.0%
TCATGTGAATTCATAGTTGA+AGG + chr1_3:75562399-75562418 None:intergenic 30.0%
! ATAACCCTCATGATAGTTTT+AGG + chr1_3:75562667-75562686 None:intergenic 30.0%
TAAAACTATCATGAGGGTTA+TGG - chr1_3:75562666-75562685 Msa0140910:CDS 30.0%
AAAACTATCATGAGGGTTAT+GGG - chr1_3:75562667-75562686 Msa0140910:CDS 30.0%
TTTGTATTCAAACCTGAAGA+TGG + chr1_3:75562726-75562745 None:intergenic 30.0%
CTTGTACAAAAATTTGCAAC+CGG - chr1_3:75562850-75562869 Msa0140910:CDS 30.0%
AATGATCACTCAATTAAGGA+TGG - chr1_3:75563080-75563099 Msa0140910:CDS 30.0%
! AGAGATGTATAATGGAAGAA+GGG + chr1_3:75563310-75563329 None:intergenic 30.0%
! AAGAGATGTATAATGGAAGA+AGG + chr1_3:75563311-75563330 None:intergenic 30.0%
!! GTTTTGAATCTTTTTGTTGG+GGG - chr1_3:75563404-75563423 Msa0140910:intron 30.0%
!!! TTTGTTTTGGAATGTATGTG+TGG - chr1_3:75563437-75563456 Msa0140910:intron 30.0%
TTGAATTAGTAACTCACACA+AGG - chr1_3:75563466-75563485 Msa0140910:CDS 30.0%
! TGCACATATATTTGGAATCA+TGG - chr1_3:75563591-75563610 Msa0140910:intron 30.0%
GTTTGACGAAATTATAACGA+TGG - chr1_3:75563617-75563636 Msa0140910:intron 30.0%
TGAAAAACTATGCACTTTGA+AGG + chr1_3:75563661-75563680 None:intergenic 30.0%
ATGGGACTATTGAAAGAATT+TGG - chr1_3:75563982-75564001 Msa0140910:intron 30.0%
! TTTCAAGATCTTGACCTTTT+CGG - chr1_3:75564027-75564046 Msa0140910:intron 30.0%
CATATATGCATGATGATCTA+GGG + chr1_3:75564106-75564125 None:intergenic 30.0%
ATGGAAAAAACCCATTAATG+TGG - chr1_3:75564193-75564212 Msa0140910:intron 30.0%
AATCATCACTCCACATTAAT+GGG + chr1_3:75564206-75564225 None:intergenic 30.0%
AAATCATCACTCCACATTAA+TGG + chr1_3:75564207-75564226 None:intergenic 30.0%
TAATGTGGAGTGATGATTTA+AGG - chr1_3:75564208-75564227 Msa0140910:intron 30.0%
! GATTTAAGGCTTCAAGATTT+AGG - chr1_3:75564222-75564241 Msa0140910:intron 30.0%
TGAATTTGATCACCTTTGAA+AGG + chr1_3:75564273-75564292 None:intergenic 30.0%
ATACAAAGCCTTTACTTCAA+GGG - chr1_3:75564349-75564368 Msa0140910:intron 30.0%
AAAATTTCCCCTTGAAGTAA+AGG + chr1_3:75564360-75564379 None:intergenic 30.0%
! TTACTTCAAGGGGAAATTTT+AGG - chr1_3:75564360-75564379 Msa0140910:intron 30.0%
ATAGTACCAGTACTAATCAT+AGG + chr1_3:75564471-75564490 None:intergenic 30.0%
!! TATGATTAGTACTGGTACTA+TGG - chr1_3:75564470-75564489 Msa0140910:CDS 30.0%
! TTTGTTGTGAAACTCTCTTA+GGG - chr1_3:75564583-75564602 Msa0140910:CDS 30.0%
GAAATCATCATGTTCTTATG+AGG - chr1_3:75564623-75564642 Msa0140910:exon 30.0%
GAAGAGAAAATGGAAGTGAA+TGG + chr1_3:75562272-75562291 None:intergenic 35.0%
ACAAATGCGGATGCAAAATA+GGG + chr1_3:75562317-75562336 None:intergenic 35.0%
AACAAATGCGGATGCAAAAT+AGG + chr1_3:75562318-75562337 None:intergenic 35.0%
CAGAGATTCAAGAACAAATG+CGG + chr1_3:75562330-75562349 None:intergenic 35.0%
CATTTGTTCTTGAATCTCTG+CGG - chr1_3:75562330-75562349 Msa0140910:three_prime_UTR 35.0%
TTCATAGTTGAAGGCTTCTT+TGG + chr1_3:75562390-75562409 None:intergenic 35.0%
GAGAAGAGAAAGAAAAAAGG+TGG + chr1_3:75562564-75562583 None:intergenic 35.0%
CACTCCTAAAACTATCATGA+GGG - chr1_3:75562660-75562679 Msa0140910:CDS 35.0%
TGATCATTGAATTCCTTGTG+GGG + chr1_3:75563068-75563087 None:intergenic 35.0%
GTGATCATTGAATTCCTTGT+GGG + chr1_3:75563069-75563088 None:intergenic 35.0%
AGTGATCATTGAATTCCTTG+TGG + chr1_3:75563070-75563089 None:intergenic 35.0%
TCAATTAAGGATGGTATGAG+AGG - chr1_3:75563089-75563108 Msa0140910:CDS 35.0%
! GTGACACTTCTTTTCTTTCT+AGG - chr1_3:75563111-75563130 Msa0140910:CDS 35.0%
ATGCATACTCATTCATGTTG+CGG + chr1_3:75563280-75563299 None:intergenic 35.0%
! GTATAATGGAAGAAGGGAAT+TGG + chr1_3:75563304-75563323 None:intergenic 35.0%
TGTAATCAGGATGCAAATTG+AGG - chr1_3:75563493-75563512 Msa0140910:intron 35.0%
TTGAAGGTTGAACGAAATCA+CGG + chr1_3:75563645-75563664 None:intergenic 35.0%
!! ATCCTTTGAAACCAGTTGTA+CGG - chr1_3:75563695-75563714 Msa0140910:intron 35.0%
!! CAAATTGGATTCTCTAAAGC+CGG + chr1_3:75563747-75563766 None:intergenic 35.0%
AACTACGAATTGAAGCTCAA+GGG - chr1_3:75563842-75563861 Msa0140910:intron 35.0%
ACTTGACCTTCAACAAAACA+TGG - chr1_3:75564071-75564090 Msa0140910:intron 35.0%
GCATATATGCATGATGATCT+AGG + chr1_3:75564107-75564126 None:intergenic 35.0%
ATCAATGAGAACTTAAGCCT+TGG - chr1_3:75564129-75564148 Msa0140910:intron 35.0%
! CACTATAAGGGTTAGTGTTA+GGG + chr1_3:75564170-75564189 None:intergenic 35.0%
! ACTAACCCTTATAGTGGAAA+TGG - chr1_3:75564174-75564193 Msa0140910:intron 35.0%
GATACAAAGCCTTTACTTCA+AGG - chr1_3:75564348-75564367 Msa0140910:intron 35.0%
TACAAAGCCTTTACTTCAAG+GGG - chr1_3:75564350-75564369 Msa0140910:intron 35.0%
ATTCTTTCAGCATGAAGAGA+TGG + chr1_3:75564444-75564463 None:intergenic 35.0%
ATGAGTCCTATGATTAGTAC+TGG - chr1_3:75564462-75564481 Msa0140910:CDS 35.0%
CTAAGAGAGTTTCACAACAA+AGG + chr1_3:75564584-75564603 None:intergenic 35.0%
! CTTTGTTGTGAAACTCTCTT+AGG - chr1_3:75564582-75564601 Msa0140910:CDS 35.0%
!!! CTCTCTTAGGGTTTTGAATT+TGG - chr1_3:75564595-75564614 Msa0140910:CDS 35.0%
!!! TAGGGTTTTGAATTTGGTCT+TGG - chr1_3:75564601-75564620 Msa0140910:CDS 35.0%
TGAGAATGCTCACATTAACT+AGG + chr1_3:75564721-75564740 None:intergenic 35.0%
TAGTTGAAGGCTTCTTTGGA+GGG + chr1_3:75562386-75562405 None:intergenic 40.0%
ATAGTTGAAGGCTTCTTTGG+AGG + chr1_3:75562387-75562406 None:intergenic 40.0%
CAGCATCAACAAAACGTTCA+TGG + chr1_3:75562503-75562522 None:intergenic 40.0%
GGTGAAAGAATAGTACTGTC+AGG + chr1_3:75562543-75562562 None:intergenic 40.0%
!!! CCTCATGATAGTTTTAGGAG+TGG + chr1_3:75562662-75562681 None:intergenic 40.0%
CCACTCCTAAAACTATCATG+AGG - chr1_3:75562659-75562678 Msa0140910:CDS 40.0%
ATGAGGGTTATGGGTGTAAA+GGG - chr1_3:75562676-75562695 Msa0140910:CDS 40.0%
ATGGCTCTTGAGATGGTAAA+GGG + chr1_3:75562707-75562726 None:intergenic 40.0%
AATCATCTCCTACGATGCAA+AGG - chr1_3:75562813-75562832 Msa0140910:CDS 40.0%
GGACAACATACTACTGTATC+CGG + chr1_3:75562872-75562891 None:intergenic 40.0%
!! CTTCAGCTTTAGAACTGCAA+CGG - chr1_3:75563225-75563244 Msa0140910:intron 40.0%
!! GGGTGAGGAGATTTTTGTTT+TGG - chr1_3:75563424-75563443 Msa0140910:intron 40.0%
!! CACACAAGGTTGATGTAATC+AGG - chr1_3:75563480-75563499 Msa0140910:intron 40.0%
AAGACTACATGAGCAACTTG+AGG - chr1_3:75563523-75563542 Msa0140910:intron 40.0%
! CAACTGGTTTCAAAGGATCT+AGG + chr1_3:75563693-75563712 None:intergenic 40.0%
GAGCTTCAATTCGTAGTTGA+AGG + chr1_3:75563840-75563859 None:intergenic 40.0%
CAACTACGAATTGAAGCTCA+AGG - chr1_3:75563841-75563860 Msa0140910:intron 40.0%
AAAGCTTATCAGACACTTGC+AGG - chr1_3:75563889-75563908 Msa0140910:intron 40.0%
!! AACTTAAAGGGCGTTGTTCT+TGG - chr1_3:75563940-75563959 Msa0140910:intron 40.0%
!! GCTTGTCCATGTTTTGTTGA+AGG + chr1_3:75564080-75564099 None:intergenic 40.0%
ACTTAAGCCTTGGCAAGAAA+AGG - chr1_3:75564139-75564158 Msa0140910:intron 40.0%
! TAAGGGTTAGTGTTAGGGTT+AGG + chr1_3:75564165-75564184 None:intergenic 40.0%
! CCACTATAAGGGTTAGTGTT+AGG + chr1_3:75564171-75564190 None:intergenic 40.0%
CCTAACACTAACCCTTATAG+TGG - chr1_3:75564168-75564187 Msa0140910:intron 40.0%
TGCTTGGATGATCCTTTCAA+AGG - chr1_3:75564258-75564277 Msa0140910:intron 40.0%
TCAAATTGCACCACCATCAT+TGG - chr1_3:75564290-75564309 Msa0140910:CDS 40.0%
TCACTTCCTCTATCCAATGA+TGG + chr1_3:75564306-75564325 None:intergenic 40.0%
ATGAGGTATAATGCCTTGCA+TGG - chr1_3:75564640-75564659 Msa0140910:five_prime_UTR 40.0%
CGGTATTCTCTTCACAGAGA+TGG - chr1_3:75562350-75562369 Msa0140910:three_prime_UTR 45.0%
GATAGACCCATGTGTGTTCA+AGG - chr1_3:75562417-75562436 Msa0140910:three_prime_UTR 45.0%
GAGTCACCTTGAACACACAT+GGG + chr1_3:75562426-75562445 None:intergenic 45.0%
TGAGTCACCTTGAACACACA+TGG + chr1_3:75562427-75562446 None:intergenic 45.0%
ATGTGTGTTCAAGGTGACTC+AGG - chr1_3:75562426-75562445 Msa0140910:three_prime_UTR 45.0%
! TCCAACGAAGACGAGGTTTT+GGG + chr1_3:75562470-75562489 None:intergenic 45.0%
GTTGATGCTGTTACTCAGCT+TGG - chr1_3:75562513-75562532 Msa0140910:CDS 45.0%
CATGAGGGTTATGGGTGTAA+AGG - chr1_3:75562675-75562694 Msa0140910:CDS 45.0%
GATGGCTCTTGAGATGGTAA+AGG + chr1_3:75562708-75562727 None:intergenic 45.0%
ACGGAATACTGCTTCCTCTT+CGG - chr1_3:75563244-75563263 Msa0140910:intron 45.0%
! GAATCTTTTTGTTGGGGGTG+AGG - chr1_3:75563409-75563428 Msa0140910:intron 45.0%
! CACCGTACAACTGGTTTCAA+AGG + chr1_3:75563700-75563719 None:intergenic 45.0%
AATTGACTGCACCGTACAAC+TGG + chr1_3:75563709-75563728 None:intergenic 45.0%
GACACTTGCAGGTGAAAACA+TGG - chr1_3:75563900-75563919 Msa0140910:CDS 45.0%
TGTTCTTGGACCTCATGGAA+TGG - chr1_3:75563954-75563973 Msa0140910:intron 45.0%
ACCTCATGGAATGGCTGAAA+TGG - chr1_3:75563963-75563982 Msa0140910:intron 45.0%
CCTCATGGAATGGCTGAAAT+GGG - chr1_3:75563964-75563983 Msa0140910:intron 45.0%
! CAAGATCTTGACCTTTTCGG+AGG - chr1_3:75564030-75564049 Msa0140910:intron 45.0%
CTTCCTCTATCCAATGATGG+TGG + chr1_3:75564303-75564322 None:intergenic 45.0%
AAAGACCCTCACCAAGAAGA+GGG - chr1_3:75564418-75564437 Msa0140910:intron 45.0%
!! AGTACTGGTACTATGGCACA+AGG - chr1_3:75564477-75564496 Msa0140910:CDS 45.0%
!! AAACGTTGGTTTGCCATGCA+AGG + chr1_3:75564656-75564675 None:intergenic 45.0%
!! CAAAAAATTAAAAAAAAATT+TGG + chr1_3:75563139-75563158 None:intergenic 5.0%
ACCCAAAACCTCGTCTTCGT+TGG - chr1_3:75562466-75562485 Msa0140910:exon 50.0%
! GTCCAACGAAGACGAGGTTT+TGG + chr1_3:75562471-75562490 None:intergenic 50.0%
TCAACAGTCCAACGAAGACG+AGG + chr1_3:75562477-75562496 None:intergenic 50.0%
GATGCTGTTACTCAGCTTGG+AGG - chr1_3:75562516-75562535 Msa0140910:CDS 50.0%
CCTGAAGATGGCTCTTGAGA+TGG + chr1_3:75562714-75562733 None:intergenic 50.0%
CCATCTCAAGAGCCATCTTC+AGG - chr1_3:75562711-75562730 Msa0140910:CDS 50.0%
TTGCTGCACCTTTGCATCGT+AGG + chr1_3:75562824-75562843 None:intergenic 50.0%
GCTTCCTCTTCGGCTATGAT+TGG - chr1_3:75563254-75563273 Msa0140910:intron 50.0%
CCCATTTCAGCCATTCCATG+AGG + chr1_3:75563967-75563986 None:intergenic 50.0%
! GATCTTGACCTTTTCGGAGG+CGG - chr1_3:75564033-75564052 Msa0140910:intron 50.0%
! GGTTAGGCCTTTTCTTGCCA+AGG + chr1_3:75564149-75564168 None:intergenic 50.0%
AGGGACAGCTTCATCATGCT+TGG - chr1_3:75564242-75564261 Msa0140910:intron 50.0%
GCACCACCATCATTGGATAG+AGG - chr1_3:75564297-75564316 Msa0140910:CDS 50.0%
GAAAGACCCTCACCAAGAAG+AGG - chr1_3:75564417-75564436 Msa0140910:intron 50.0%
TGAAGAGATGGCCCTCTTCT+TGG + chr1_3:75564432-75564451 None:intergenic 50.0%
GGTACTATGGCACAAGGTAG+AGG - chr1_3:75564483-75564502 Msa0140910:CDS 50.0%
CAAGGTAGAGGCTCACCATT+TGG - chr1_3:75564495-75564514 Msa0140910:CDS 50.0%
GCTTGGAAAGCAACCCCACA+AGG - chr1_3:75563052-75563071 Msa0140910:CDS 55.0%
GGCGTTGTTCTTGGACCTCA+TGG - chr1_3:75563949-75563968 Msa0140910:intron 55.0%
GATGGCCCTCTTCTTGGTGA+GGG + chr1_3:75564426-75564445 None:intergenic 55.0%
AGATGGCCCTCTTCTTGGTG+AGG + chr1_3:75564427-75564446 None:intergenic 55.0%
TCATCTCTCACGCTGCGCAC+CGG + chr1_3:75562918-75562937 None:intergenic 60.0%
GCGGCCAATCATAGCCGAAG+AGG + chr1_3:75563261-75563280 None:intergenic 60.0%
! CTTGACCTTTTCGGAGGCGG+TGG - chr1_3:75564036-75564055 Msa0140910:intron 60.0%
! GACCTTTTCGGAGGCGGTGG+TGG - chr1_3:75564039-75564058 Msa0140910:intron 65.0%
CACCACCACCGCCTCCGAAA+AGG + chr1_3:75564044-75564063 None:intergenic 65.0%
!! CTTTTCGGAGGCGGTGGTGG+TGG - chr1_3:75564042-75564061 Msa0140910:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 75562213 75564781 75562213 ID=Msa0140910;Name=Msa0140910
chr1_3 mRNA 75562213 75564781 75562213 ID=Msa0140910-mRNA-1;Parent=Msa0140910;Name=Msa0140910-mRNA-1;_AED=0.07;_eAED=0.07;_QI=156|0.8|0.83|1|0.8|0.83|6|260|372
chr1_3 exon 75562213 75563168 75562213 ID=Msa0140910-mRNA-1:exon:26353;Parent=Msa0140910-mRNA-1
chr1_3 exon 75563450 75563492 75563450 ID=Msa0140910-mRNA-1:exon:26352;Parent=Msa0140910-mRNA-1
chr1_3 exon 75563703 75563769 75563703 ID=Msa0140910-mRNA-1:exon:26351;Parent=Msa0140910-mRNA-1
chr1_3 exon 75563893 75563950 75563893 ID=Msa0140910-mRNA-1:exon:26350;Parent=Msa0140910-mRNA-1
chr1_3 exon 75564262 75564338 75564262 ID=Msa0140910-mRNA-1:exon:26349;Parent=Msa0140910-mRNA-1
chr1_3 exon 75564448 75564781 75564448 ID=Msa0140910-mRNA-1:exon:26348;Parent=Msa0140910-mRNA-1
chr1_3 five_prime_UTR 75564626 75564781 75564626 ID=Msa0140910-mRNA-1:five_prime_utr;Parent=Msa0140910-mRNA-1
chr1_3 CDS 75564448 75564625 75564448 ID=Msa0140910-mRNA-1:cds;Parent=Msa0140910-mRNA-1
chr1_3 CDS 75564262 75564338 75564262 ID=Msa0140910-mRNA-1:cds;Parent=Msa0140910-mRNA-1
chr1_3 CDS 75563893 75563950 75563893 ID=Msa0140910-mRNA-1:cds;Parent=Msa0140910-mRNA-1
chr1_3 CDS 75563703 75563769 75563703 ID=Msa0140910-mRNA-1:cds;Parent=Msa0140910-mRNA-1
chr1_3 CDS 75563450 75563492 75563450 ID=Msa0140910-mRNA-1:cds;Parent=Msa0140910-mRNA-1
chr1_3 CDS 75562473 75563168 75562473 ID=Msa0140910-mRNA-1:cds;Parent=Msa0140910-mRNA-1
chr1_3 three_prime_UTR 75562213 75562472 75562213 ID=Msa0140910-mRNA-1:three_prime_utr;Parent=Msa0140910-mRNA-1
Gene Sequence

>Msa0140910

ATGGAAAGAATGTTCCCTCCAAAGAAGCCTTCAACTATGAATTCACATGATAGACCCATGTGTGTTCAAGGTGACTCAGGTCTTGTTCTTACAACTGACCCAAAACCTCGTCTTCGTTGGACTGTTGAACTCCATGAACGTTTTGTTGATGCTGTTACTCAGCTTGGAGGACCTGACAAGGCCACTCCTAAAACTATCATGAGGGTTATGGGTGTAAAGGGTCTTACCCTTTACCATCTCAAGAGCCATCTTCAGAAATTTAGGCTTGGAAAGCAACCCCACAAGGAATTCAATGATCACTCAATTAAGGATGCTTCAGCTTTAGAACTGCAACGGAATACTGCTTCCTCTTCGGCTATGATTGGCCGCAACATGAATGAGATGCAAATTGAGGTGCAGAGAAGACTACATGAGCAACTTGAGGTACAAAAACACCTTCAACTACGAATTGAAGCTCAAGGGAAATACATGCAAAGTATATTGGAAAAAGCTTATCAGACACTTGCAGGTGAAAACATGGCATCTGCTACAACTAATAACTTAAAGGGCGTTGTTCTTGGACCTCATGGAATGGCTGAAATGGGACTATTGAAAGAATTTGGTTCACCTCTTAGTTTTACATCTTTTCAAGATCTTGACCTTTTCGGAGGCGGTGGTGGTGGTGATCAACTTGACCTTCAACAAAACATGGACAAGCAAACCCTAGATCATCATGCATATATGCAAATCAATGAGAACTTAAGCCTTGGCAAGAAAAGGCCTAACCCTAACACTAACCCTTATAGTGGAAATGGAAAAAACCCATTAATGTGGAGTGATGATTTAAGGCTTCAAGATTTAGGGACAGCTTCATCATGCTTGGATGATCCTTTCAAAGGTGATCAAATTCAAATTGCACCACCATCATTGGATAGAGGAAGTGATATTGAAACTATAGATATTTATGATACAAAGCCTTTACTTCAAGGGGAAATTTTAGGTGAAAAGAAATTTGATGCATCTATGAATAAACTTGAAAGACCCTCACCAAGAAGAGGGCCATCTCTTCATGCTGAAAGAATGAGTCCTATGATTAGTACTGGTACTATGGCACAAGGTAGAGGCTCACCATTTGGATGA
Protein sequence

>Msa0140910

MERMFPPKKPSTMNSHDRPMCVQGDSGLVLTTDPKPRLRWTVELHERFVDAVTQLGGPDKATPKTIMRVMGVKGLTLYHLKSHLQKFRLGKQPHKEFNDHSIKDASALELQRNTASSSAMIGRNMNEMQIEVQRRLHEQLEVQKHLQLRIEAQGKYMQSILEKAYQTLAGENMASATTNNLKGVVLGPHGMAEMGLLKEFGSPLSFTSFQDLDLFGGGGGGDQLDLQQNMDKQTLDHHAYMQINENLSLGKKRPNPNTNPYSGNGKNPLMWSDDLRLQDLGTASSCLDDPFKGDQIQIAPPSLDRGSDIETIDIYDTKPLLQGEILGEKKFDASMNKLERPSPRRGPSLHAERMSPMISTGTMAQGRGSPFG*