AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0191190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0191190 MtrunA17_Chr2g0281121 93.487 261 13 2 1 260 14 271 5.52e-168 465
Msa0191190 MtrunA17_Chr4g0048371 53.160 269 93 10 1 249 24 279 3.08e-47 158
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0191190 AT4G21500.1 34.979 243 100 7 17 240 12 215 3.55e-18 80.9

Find 41 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAAGTTGAAATCTAAAA+TGG 0.203889 2_1:-6731824 Msa0191190:CDS
TCGAGACCCACTTGAAAATT+TGG 0.233268 2_1:+6731857 None:intergenic
CACCGGAGAATATGAATTTC+CGG 0.299531 2_1:-6731585 Msa0191190:CDS
GAACTATCTGAATCAGATTC+AGG 0.336496 2_1:+6731646 None:intergenic
TTTCTTCACTCTTAGATCTC+TGG 0.338598 2_1:+6731977 None:intergenic
TTCATCGGAGAATGAGTTTC+CGG 0.350752 2_1:-6731626 Msa0191190:CDS
AGTTTCCGGCGATGGAGAAC+TGG 0.362497 2_1:-6731612 Msa0191190:CDS
GGCTTTAAGAGGAAGGCTTT+GGG 0.375043 2_1:-6731517 Msa0191190:CDS
TGGCTTTAAGAGGAAGGCTT+TGG 0.384066 2_1:-6731518 Msa0191190:CDS
GAGATCTAAGAGTGAAGAAA+AGG 0.411423 2_1:-6731974 Msa0191190:CDS
TTAATTGAGATAGCTCTTGA+TGG 0.421762 2_1:-6731538 Msa0191190:CDS
TCTAATGAGAATTGTGTTGC+TGG 0.422953 2_1:-6731703 Msa0191190:CDS
ACATTTCTCCGACGAAGCGC+AGG 0.439702 2_1:-6731459 Msa0191190:CDS
TAGCTCTTGATGGCTTTAAG+AGG 0.465176 2_1:-6731528 Msa0191190:CDS
TCACCAGTTCTAATTCTCAC+TGG 0.478806 2_1:-6732036 Msa0191190:CDS
GGAGAATGAGTTTCCGGCGA+TGG 0.484875 2_1:-6731620 Msa0191190:CDS
GAAAAGTTGAAATCTAAAAT+GGG 0.488091 2_1:-6731823 Msa0191190:CDS
CTCTATCTTCTTCATCCCAC+CGG 0.498324 2_1:+6731566 None:intergenic
GGCTTTGGGATTTCAGTTTG+AGG 0.516822 2_1:-6731503 Msa0191190:CDS
CACCGGAAATTCATATTCTC+CGG 0.520892 2_1:+6731583 None:intergenic
ATGGAACGTGAAAGCGCCGT+TGG 0.537116 2_1:-6731761 Msa0191190:CDS
GAAAACTCCCTGCGCTTCGT+CGG 0.545300 2_1:+6731451 None:intergenic
TCTTGATGGCTTTAAGAGGA+AGG 0.545888 2_1:-6731524 Msa0191190:CDS
GGGATGAAGAAGATAGAGAA+GGG 0.548429 2_1:-6731561 Msa0191190:CDS
GATGGAGAACTGGGACTCAC+CGG 0.552428 2_1:-6731602 Msa0191190:CDS
ACACCAGTGAGAATTAGAAC+TGG 0.561591 2_1:+6732033 None:intergenic
GAGTCCCAGTTCTCCATCGC+CGG 0.564533 2_1:+6731607 None:intergenic
GATGAGAATCAAGGTGCAGA+AGG 0.566008 2_1:+6732092 None:intergenic
TCTTCATATATAACTTCCAA+CGG 0.567961 2_1:+6731745 None:intergenic
AGGAAAATAGATGAGAATCA+AGG 0.575079 2_1:+6732083 None:intergenic
GTTTCCGGCGATGGAGAACT+GGG 0.578488 2_1:-6731611 Msa0191190:CDS
GGAAAACACCTCCTCCTCGC+CGG 0.581509 2_1:+6731429 None:intergenic
TTAATTCCAACTCAAATCAC+CGG 0.596284 2_1:+6731408 None:intergenic
CATTTCTCCGACGAAGCGCA+GGG 0.607667 2_1:-6731458 Msa0191190:CDS
TGGGATGAAGAAGATAGAGA+AGG 0.615054 2_1:-6731562 Msa0191190:CDS
CGGAGAATATGAATTTCCGG+TGG 0.615835 2_1:-6731582 Msa0191190:CDS
ATCTGATTCAGATAGTTCAT+CGG 0.620524 2_1:-6731641 Msa0191190:CDS
ATATGAAGATGAAGAAACAG+AGG 0.620651 2_1:-6731731 Msa0191190:CDS
GGGGAAAACAGAGGAAAATG+TGG 0.620751 2_1:-6731896 Msa0191190:CDS
GGAGAATATGAATTTCCGGT+GGG 0.667099 2_1:-6731581 Msa0191190:CDS
GTAATAATGCATGAAAACAG+AGG 0.676002 2_1:+6732114 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAGATTTCAACTTTTCTT+CGG + chr2_1:6731751-6731770 None:intergenic 20.0%
!! AGAAAAGTTGAAATCTAAAA+TGG - chr2_1:6731752-6731771 Msa0191190:CDS 20.0%
!!! GAAAAGTTGAAATCTAAAAT+GGG - chr2_1:6731753-6731772 Msa0191190:CDS 20.0%
!!! TTTAGATTTCAACTTTTCTT+CGG + chr2_1:6731751-6731770 None:intergenic 20.0%
!! AGAAAAGTTGAAATCTAAAA+TGG - chr2_1:6731752-6731771 Msa0191190:CDS 20.0%
!!! GAAAAGTTGAAATCTAAAAT+GGG - chr2_1:6731753-6731772 Msa0191190:CDS 20.0%
!! ACTATTTTGCAGAATGAATA+AGG + chr2_1:6731516-6731535 None:intergenic 25.0%
!! TTGATTTTTGTACTTCGTTT+TGG - chr2_1:6731570-6731589 Msa0191190:CDS 25.0%
! AGTGAAGAAAAGGAAAAATT+TGG - chr2_1:6731612-6731631 Msa0191190:CDS 25.0%
! TCTTCATATATAACTTCCAA+CGG + chr2_1:6731834-6731853 None:intergenic 25.0%
!! ACTATTTTGCAGAATGAATA+AGG + chr2_1:6731516-6731535 None:intergenic 25.0%
!! TTGATTTTTGTACTTCGTTT+TGG - chr2_1:6731570-6731589 Msa0191190:CDS 25.0%
! AGTGAAGAAAAGGAAAAATT+TGG - chr2_1:6731612-6731631 Msa0191190:CDS 25.0%
! TCTTCATATATAACTTCCAA+CGG + chr2_1:6731834-6731853 None:intergenic 25.0%
GTAATAATGCATGAAAACAG+AGG + chr2_1:6731465-6731484 None:intergenic 30.0%
AGGAAAATAGATGAGAATCA+AGG + chr2_1:6731496-6731515 None:intergenic 30.0%
! TTCATCTCTGTTTTCTTCAT+TGG + chr2_1:6731655-6731674 None:intergenic 30.0%
AGAAAACAGAGATGAAAAAC+AGG - chr2_1:6731659-6731678 Msa0191190:CDS 30.0%
GAAAACAGAGATGAAAAACA+GGG - chr2_1:6731660-6731679 Msa0191190:CDS 30.0%
AAAACAGAGATGAAAAACAG+GGG - chr2_1:6731661-6731680 Msa0191190:CDS 30.0%
!!! TCTTGACCAAATTTTCAAGT+GGG - chr2_1:6731713-6731732 Msa0191190:CDS 30.0%
! TTGATGAGTCTTTTGTTGAA+TGG - chr2_1:6731796-6731815 Msa0191190:CDS 30.0%
ATATGAAGATGAAGAAACAG+AGG - chr2_1:6731845-6731864 Msa0191190:CDS 30.0%
ATCTGATTCAGATAGTTCAT+CGG - chr2_1:6731935-6731954 Msa0191190:CDS 30.0%
TTAATTGAGATAGCTCTTGA+TGG - chr2_1:6732038-6732057 Msa0191190:CDS 30.0%
TTAATTCCAACTCAAATCAC+CGG + chr2_1:6732171-6732190 None:intergenic 30.0%
GTAATAATGCATGAAAACAG+AGG + chr2_1:6731465-6731484 None:intergenic 30.0%
AGGAAAATAGATGAGAATCA+AGG + chr2_1:6731496-6731515 None:intergenic 30.0%
! TTCATCTCTGTTTTCTTCAT+TGG + chr2_1:6731655-6731674 None:intergenic 30.0%
AGAAAACAGAGATGAAAAAC+AGG - chr2_1:6731659-6731678 Msa0191190:CDS 30.0%
GAAAACAGAGATGAAAAACA+GGG - chr2_1:6731660-6731679 Msa0191190:CDS 30.0%
AAAACAGAGATGAAAAACAG+GGG - chr2_1:6731661-6731680 Msa0191190:CDS 30.0%
!!! TCTTGACCAAATTTTCAAGT+GGG - chr2_1:6731713-6731732 Msa0191190:CDS 30.0%
! TTGATGAGTCTTTTGTTGAA+TGG - chr2_1:6731796-6731815 Msa0191190:CDS 30.0%
ATATGAAGATGAAGAAACAG+AGG - chr2_1:6731845-6731864 Msa0191190:CDS 30.0%
ATCTGATTCAGATAGTTCAT+CGG - chr2_1:6731935-6731954 Msa0191190:CDS 30.0%
TTAATTGAGATAGCTCTTGA+TGG - chr2_1:6732038-6732057 Msa0191190:CDS 30.0%
TTAATTCCAACTCAAATCAC+CGG + chr2_1:6732171-6732190 None:intergenic 30.0%
TTTCTTCACTCTTAGATCTC+TGG + chr2_1:6731602-6731621 None:intergenic 35.0%
GAGATCTAAGAGTGAAGAAA+AGG - chr2_1:6731602-6731621 Msa0191190:CDS 35.0%
!! CTCTTGACCAAATTTTCAAG+TGG - chr2_1:6731712-6731731 Msa0191190:CDS 35.0%
TCTAATGAGAATTGTGTTGC+TGG - chr2_1:6731873-6731892 Msa0191190:CDS 35.0%
GAACTATCTGAATCAGATTC+AGG + chr2_1:6731933-6731952 None:intergenic 35.0%
TTTCTTCACTCTTAGATCTC+TGG + chr2_1:6731602-6731621 None:intergenic 35.0%
GAGATCTAAGAGTGAAGAAA+AGG - chr2_1:6731602-6731621 Msa0191190:CDS 35.0%
!! CTCTTGACCAAATTTTCAAG+TGG - chr2_1:6731712-6731731 Msa0191190:CDS 35.0%
TCTAATGAGAATTGTGTTGC+TGG - chr2_1:6731873-6731892 Msa0191190:CDS 35.0%
GAACTATCTGAATCAGATTC+AGG + chr2_1:6731933-6731952 None:intergenic 35.0%
! TCACCAGTTCTAATTCTCAC+TGG - chr2_1:6731540-6731559 Msa0191190:CDS 40.0%
ACACCAGTGAGAATTAGAAC+TGG + chr2_1:6731546-6731565 None:intergenic 40.0%
TGAAAAACAGGGGAAAACAG+AGG - chr2_1:6731671-6731690 Msa0191190:CDS 40.0%
TCGAGACCCACTTGAAAATT+TGG + chr2_1:6731722-6731741 None:intergenic 40.0%
TTCATCGGAGAATGAGTTTC+CGG - chr2_1:6731950-6731969 Msa0191190:CDS 40.0%
CACCGGAGAATATGAATTTC+CGG - chr2_1:6731991-6732010 Msa0191190:CDS 40.0%
CACCGGAAATTCATATTCTC+CGG + chr2_1:6731996-6732015 None:intergenic 40.0%
GGAGAATATGAATTTCCGGT+GGG - chr2_1:6731995-6732014 Msa0191190:CDS 40.0%
TGGGATGAAGAAGATAGAGA+AGG - chr2_1:6732014-6732033 Msa0191190:CDS 40.0%
GGGATGAAGAAGATAGAGAA+GGG - chr2_1:6732015-6732034 Msa0191190:CDS 40.0%
!! TAGCTCTTGATGGCTTTAAG+AGG - chr2_1:6732048-6732067 Msa0191190:CDS 40.0%
!! TCTTGATGGCTTTAAGAGGA+AGG - chr2_1:6732052-6732071 Msa0191190:CDS 40.0%
! TGTTTTCCGGTGATTTGAGT+TGG - chr2_1:6732162-6732181 Msa0191190:CDS 40.0%
! TCACCAGTTCTAATTCTCAC+TGG - chr2_1:6731540-6731559 Msa0191190:CDS 40.0%
ACACCAGTGAGAATTAGAAC+TGG + chr2_1:6731546-6731565 None:intergenic 40.0%
TGAAAAACAGGGGAAAACAG+AGG - chr2_1:6731671-6731690 Msa0191190:CDS 40.0%
TCGAGACCCACTTGAAAATT+TGG + chr2_1:6731722-6731741 None:intergenic 40.0%
TTCATCGGAGAATGAGTTTC+CGG - chr2_1:6731950-6731969 Msa0191190:CDS 40.0%
CACCGGAGAATATGAATTTC+CGG - chr2_1:6731991-6732010 Msa0191190:CDS 40.0%
CACCGGAAATTCATATTCTC+CGG + chr2_1:6731996-6732015 None:intergenic 40.0%
GGAGAATATGAATTTCCGGT+GGG - chr2_1:6731995-6732014 Msa0191190:CDS 40.0%
TGGGATGAAGAAGATAGAGA+AGG - chr2_1:6732014-6732033 Msa0191190:CDS 40.0%
GGGATGAAGAAGATAGAGAA+GGG - chr2_1:6732015-6732034 Msa0191190:CDS 40.0%
!! TAGCTCTTGATGGCTTTAAG+AGG - chr2_1:6732048-6732067 Msa0191190:CDS 40.0%
!! TCTTGATGGCTTTAAGAGGA+AGG - chr2_1:6732052-6732071 Msa0191190:CDS 40.0%
! TGTTTTCCGGTGATTTGAGT+TGG - chr2_1:6732162-6732181 Msa0191190:CDS 40.0%
GATGAGAATCAAGGTGCAGA+AGG + chr2_1:6731487-6731506 None:intergenic 45.0%
GGGGAAAACAGAGGAAAATG+TGG - chr2_1:6731680-6731699 Msa0191190:CDS 45.0%
CGGAGAATATGAATTTCCGG+TGG - chr2_1:6731994-6732013 Msa0191190:CDS 45.0%
CTCTATCTTCTTCATCCCAC+CGG + chr2_1:6732013-6732032 None:intergenic 45.0%
!! TGGCTTTAAGAGGAAGGCTT+TGG - chr2_1:6732058-6732077 Msa0191190:CDS 45.0%
!! GGCTTTAAGAGGAAGGCTTT+GGG - chr2_1:6732059-6732078 Msa0191190:CDS 45.0%
!! GGCTTTGGGATTTCAGTTTG+AGG - chr2_1:6732073-6732092 Msa0191190:CDS 45.0%
GATGAGAATCAAGGTGCAGA+AGG + chr2_1:6731487-6731506 None:intergenic 45.0%
GGGGAAAACAGAGGAAAATG+TGG - chr2_1:6731680-6731699 Msa0191190:CDS 45.0%
CGGAGAATATGAATTTCCGG+TGG - chr2_1:6731994-6732013 Msa0191190:CDS 45.0%
CTCTATCTTCTTCATCCCAC+CGG + chr2_1:6732013-6732032 None:intergenic 45.0%
!! TGGCTTTAAGAGGAAGGCTT+TGG - chr2_1:6732058-6732077 Msa0191190:CDS 45.0%
!! GGCTTTAAGAGGAAGGCTTT+GGG - chr2_1:6732059-6732078 Msa0191190:CDS 45.0%
!! GGCTTTGGGATTTCAGTTTG+AGG - chr2_1:6732073-6732092 Msa0191190:CDS 45.0%
!! ATGGAACGTGAAAGCGCCGT+TGG - chr2_1:6731815-6731834 Msa0191190:CDS 55.0%
GGAGAATGAGTTTCCGGCGA+TGG - chr2_1:6731956-6731975 Msa0191190:CDS 55.0%
AGTTTCCGGCGATGGAGAAC+TGG - chr2_1:6731964-6731983 Msa0191190:CDS 55.0%
GTTTCCGGCGATGGAGAACT+GGG - chr2_1:6731965-6731984 Msa0191190:CDS 55.0%
GATGGAGAACTGGGACTCAC+CGG - chr2_1:6731974-6731993 Msa0191190:CDS 55.0%
ACATTTCTCCGACGAAGCGC+AGG - chr2_1:6732117-6732136 Msa0191190:CDS 55.0%
CATTTCTCCGACGAAGCGCA+GGG - chr2_1:6732118-6732137 Msa0191190:CDS 55.0%
GAAAACTCCCTGCGCTTCGT+CGG + chr2_1:6732128-6732147 None:intergenic 55.0%
! ACGAAGCGCAGGGAGTTTTC+CGG - chr2_1:6732128-6732147 Msa0191190:CDS 55.0%
!! ATGGAACGTGAAAGCGCCGT+TGG - chr2_1:6731815-6731834 Msa0191190:CDS 55.0%
GGAGAATGAGTTTCCGGCGA+TGG - chr2_1:6731956-6731975 Msa0191190:CDS 55.0%
AGTTTCCGGCGATGGAGAAC+TGG - chr2_1:6731964-6731983 Msa0191190:CDS 55.0%
GTTTCCGGCGATGGAGAACT+GGG - chr2_1:6731965-6731984 Msa0191190:CDS 55.0%
GATGGAGAACTGGGACTCAC+CGG - chr2_1:6731974-6731993 Msa0191190:CDS 55.0%
ACATTTCTCCGACGAAGCGC+AGG - chr2_1:6732117-6732136 Msa0191190:CDS 55.0%
CATTTCTCCGACGAAGCGCA+GGG - chr2_1:6732118-6732137 Msa0191190:CDS 55.0%
GAAAACTCCCTGCGCTTCGT+CGG + chr2_1:6732128-6732147 None:intergenic 55.0%
! ACGAAGCGCAGGGAGTTTTC+CGG - chr2_1:6732128-6732147 Msa0191190:CDS 55.0%
GAGTCCCAGTTCTCCATCGC+CGG + chr2_1:6731972-6731991 None:intergenic 60.0%
GGAAAACACCTCCTCCTCGC+CGG + chr2_1:6732150-6732169 None:intergenic 60.0%
!!! GGCGAGGAGGAGGTGTTTTC+CGG - chr2_1:6732149-6732168 Msa0191190:CDS 60.0%
GAGTCCCAGTTCTCCATCGC+CGG + chr2_1:6731972-6731991 None:intergenic 60.0%
GGAAAACACCTCCTCCTCGC+CGG + chr2_1:6732150-6732169 None:intergenic 60.0%
!!! GGCGAGGAGGAGGTGTTTTC+CGG - chr2_1:6732149-6732168 Msa0191190:CDS 60.0%
! CAGGGAGTTTTCCGGCGAGG+AGG - chr2_1:6732136-6732155 Msa0191190:CDS 65.0%
! GGAGTTTTCCGGCGAGGAGG+AGG - chr2_1:6732139-6732158 Msa0191190:CDS 65.0%
! CAGGGAGTTTTCCGGCGAGG+AGG - chr2_1:6732136-6732155 Msa0191190:CDS 65.0%
! GGAGTTTTCCGGCGAGGAGG+AGG - chr2_1:6732139-6732158 Msa0191190:CDS 65.0%
! GCGCAGGGAGTTTTCCGGCG+AGG - chr2_1:6732133-6732152 Msa0191190:CDS 70.0%
! GCGCAGGGAGTTTTCCGGCG+AGG - chr2_1:6732133-6732152 Msa0191190:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 6731408 6732190 6731408 ID=Msa0191190;Name=Msa0191190
chr2_1 mRNA 6731408 6732190 6731408 ID=Msa0191190-mRNA-1;Parent=Msa0191190;Name=Msa0191190-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|260
chr2_1 exon 6731408 6732190 6731408 ID=Msa0191190-mRNA-1:exon:3079;Parent=Msa0191190-mRNA-1
chr2_1 CDS 6731408 6732190 6731408 ID=Msa0191190-mRNA-1:cds;Parent=Msa0191190-mRNA-1
Gene Sequence

>Msa0191190

ATGAACTTGTTATGCAAAGAAACTTTTAACTTTCTCTCAATTGTTTCTTCAAATCCTCTGTTTTCATGCATTATTACCTTCTGCACCTTGATTCTCATCTATTTTCCTTATTCATTCTGCAAAATAGTTTTCTCACCAGTTCTAATTCTCACTGGTGTTATATTGATTTTTGTACTTCGTTTTGGTGCAATCCAGAGATCTAAGAGTGAAGAAAAGGAAAAATTTGGTGAATTAGAATCAGTTACCAATGAAGAAAACAGAGATGAAAAACAGGGGAAAACAGAGGAAAATGTGGAAAATGATTCTCTTGACCAAATTTTCAAGTGGGTCTCGAGAAATTCCGAAGAAAAGTTGAAATCTAAAATGGGTTTTGAATCAAATTCGTTTCTTGATGAGTCTTTTGTTGAATGGAACGTGAAAGCGCCGTTGGAAGTTATATATGAAGATGAAGAAACAGAGGATATTTCTAATGAGAATTGTGTTGCTGGAATTTTGAGACACTCTTCATTGTCACGTTATTATCCTGAATCTGATTCAGATAGTTCATCGGAGAATGAGTTTCCGGCGATGGAGAACTGGGACTCACCGGAGAATATGAATTTCCGGTGGGATGAAGAAGATAGAGAAGGGTTAATTGAGATAGCTCTTGATGGCTTTAAGAGGAAGGCTTTGGGATTTCAGTTTGAGGAAGAGAATATGATTGAGATTGACATTTCTCCGACGAAGCGCAGGGAGTTTTCCGGCGAGGAGGAGGTGTTTTCCGGTGATTTGAGTTGGAATTAA

Protein sequence

>Msa0191190

MNLLCKETFNFLSIVSSNPLFSCIITFCTLILIYFPYSFCKIVFSPVLILTGVILIFVLRFGAIQRSKSEEKEKFGELESVTNEENRDEKQGKTEENVENDSLDQIFKWVSRNSEEKLKSKMGFESNSFLDESFVEWNVKAPLEVIYEDEETEDISNENCVAGILRHSSLSRYYPESDSDSSSENEFPAMENWDSPENMNFRWDEEDREGLIEIALDGFKRKALGFQFEEENMIEIDISPTKRREFSGEEEVFSGDLSWN*