AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0859200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0859200 MtrunA17_Chr5g0437991 98.710 155 2 0 1 155 25 179 3.54e-110 310
Msa0859200 MtrunA17_Chr3g0106861 46.835 158 82 1 1 156 1 158 4.13e-46 147
Msa0859200 MtrunA17_Chr7g0244441 58.537 123 50 1 27 149 6 127 2.89e-43 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0859200 AT2G40435.1 64.103 156 54 2 1 155 1 155 5.60e-68 203
Msa0859200 AT3G56220.1 57.419 155 63 3 1 153 1 154 1.34e-57 177
Msa0859200 AT2G40435.3 64.341 129 45 1 1 128 1 129 6.21e-56 172
Msa0859200 AT2G40435.2 65.354 127 43 1 1 126 1 127 6.94e-56 172
Msa0859200 AT3G56220.2 57.364 129 52 3 1 127 1 128 3.26e-44 142
Msa0859200 AT1G29270.1 35.156 128 75 5 1 123 1 125 5.56e-11 57.8

Find 31 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGTTCTGAGGCCATTTAAA+AGG 0.297363 5_4:+57370337 None:intergenic
ATTGGCAGAACTTGAAGATT+TGG 0.300801 5_4:+57368103 None:intergenic
GAAAATATATGCCGGTAGTA+AGG 0.350569 5_4:-57368074 Msa0859200:three_prime_UTR
CTCAATTACCATAGGCAAGT+TGG 0.394555 5_4:+57369489 None:intergenic
CAATACATATAAATAGATAT+TGG 0.395990 5_4:+57368222 None:intergenic
AGAAAACACATGAGATCATA+TGG 0.396404 5_4:-57370381 None:intergenic
GCCTCAGAACTCATCAGAAT+TGG 0.404408 5_4:-57370326 Msa0859200:five_prime_UTR
TCAATTACCATAGGCAAGTT+GGG 0.413845 5_4:+57369490 None:intergenic
TACAGTGGAAACCCTAGAAA+AGG 0.442701 5_4:-57368923 Msa0859200:CDS
GCTTACTGTGCTGGAAGCTA+GGG 0.457026 5_4:-57368821 Msa0859200:CDS
GGTTGCAATTTACACTTGAT+CGG 0.468244 5_4:+57368243 None:intergenic
TATTGAAAATCTAAAGGAAA+AGG 0.484456 5_4:-57369566 Msa0859200:CDS
AGAGATGAGGCTTACTGTGC+TGG 0.485736 5_4:-57368830 Msa0859200:CDS
GGCTTACTGTGCTGGAAGCT+AGG 0.491888 5_4:-57368822 Msa0859200:CDS
CCACAATCCCAACTTGCCTA+TGG 0.504234 5_4:-57369497 Msa0859200:intron
AAAGTATATTGAAAATCTAA+AGG 0.516255 5_4:-57369572 Msa0859200:CDS
AGTAAGCCTCATCTCTTCAA+TGG 0.521158 5_4:+57368837 None:intergenic
CCATAGGCAAGTTGGGATTG+TGG 0.527764 5_4:+57369497 None:intergenic
ACAGTGGAAACCCTAGAAAA+GGG 0.541907 5_4:-57368922 Msa0859200:CDS
AAAATATATGCCGGTAGTAA+GGG 0.546912 5_4:-57368073 Msa0859200:three_prime_UTR
TTACCTCTCCTCCAACAGCT+TGG 0.548657 5_4:+57368773 None:intergenic
TTTCCAAGCTGTTGGAGGAG+AGG 0.549162 5_4:-57368776 Msa0859200:intron
AACAAACGGACCCTTACTAC+CGG 0.570419 5_4:+57368063 None:intergenic
TTGAAGCCATTGAAGAGATG+AGG 0.583640 5_4:-57368843 Msa0859200:CDS
TGATGCATCGATTACGATCG+AGG 0.609202 5_4:+57369594 None:intergenic
GTATTGAAACAAGCATACCT+TGG 0.629414 5_4:+57368866 None:intergenic
ACCAATTCTGATGAGTTCTG+AGG 0.646466 5_4:+57370325 None:intergenic
TGGACTTGTGCAGGTTACAG+TGG 0.658893 5_4:-57368938 Msa0859200:intron
ACAAGCTGTGAAACAAGCCG+TGG 0.676305 5_4:-57368296 Msa0859200:CDS
CAAGCTGTGAAACAAGCCGT+GGG 0.688309 5_4:-57368295 Msa0859200:CDS
CTTATGTAGAATGAAGAACA+AGG 0.699994 5_4:-57368334 Msa0859200:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATTCCAATAATAAAAA+TGG + chr5_4:57368409-57368428 None:intergenic 10.0%
!! AAGACTATATATTATTATTA+GGG + chr5_4:57369977-57369996 None:intergenic 10.0%
!! ATTAATTCCAATAATAAAAA+TGG + chr5_4:57368409-57368428 None:intergenic 10.0%
!! AAGACTATATATTATTATTA+GGG + chr5_4:57369977-57369996 None:intergenic 10.0%
!!! GAATATTCTTTTATAGAATT+GGG + chr5_4:57368349-57368368 None:intergenic 15.0%
!!! TGAATATTCTTTTATAGAAT+TGG + chr5_4:57368350-57368369 None:intergenic 15.0%
!! TAATAAAAAAATAGCAAACT+TGG + chr5_4:57368436-57368455 None:intergenic 15.0%
!! AAAGTATATTGAAAATCTAA+AGG - chr5_4:57368868-57368887 Msa0859200:CDS 15.0%
!! AACTTATAAACAATTAAACA+TGG - chr5_4:57369176-57369195 Msa0859200:intron 15.0%
!!! TAATTCACTTTTTTTTTTCT+TGG - chr5_4:57369346-57369365 Msa0859200:intron 15.0%
!!! AATTCACTTTTTTTTTTCTT+GGG - chr5_4:57369347-57369366 Msa0859200:intron 15.0%
!! GAAGACTATATATTATTATT+AGG + chr5_4:57369978-57369997 None:intergenic 15.0%
!! CAATACATATAAATAGATAT+TGG + chr5_4:57370221-57370240 None:intergenic 15.0%
!!! GCATATATTTTCATTAAAAT+TGG + chr5_4:57370358-57370377 None:intergenic 15.0%
!!! ATTTTAATGAAAATATATGC+CGG - chr5_4:57370358-57370377 Msa0859200:five_prime_UTR 15.0%
!!! GAATATTCTTTTATAGAATT+GGG + chr5_4:57368349-57368368 None:intergenic 15.0%
!!! TGAATATTCTTTTATAGAAT+TGG + chr5_4:57368350-57368369 None:intergenic 15.0%
!! TAATAAAAAAATAGCAAACT+TGG + chr5_4:57368436-57368455 None:intergenic 15.0%
!! AAAGTATATTGAAAATCTAA+AGG - chr5_4:57368868-57368887 Msa0859200:CDS 15.0%
!! AACTTATAAACAATTAAACA+TGG - chr5_4:57369176-57369195 Msa0859200:intron 15.0%
!!! TAATTCACTTTTTTTTTTCT+TGG - chr5_4:57369346-57369365 Msa0859200:intron 15.0%
!!! AATTCACTTTTTTTTTTCTT+GGG - chr5_4:57369347-57369366 Msa0859200:intron 15.0%
!! GAAGACTATATATTATTATT+AGG + chr5_4:57369978-57369997 None:intergenic 15.0%
!! CAATACATATAAATAGATAT+TGG + chr5_4:57370221-57370240 None:intergenic 15.0%
!!! GCATATATTTTCATTAAAAT+TGG + chr5_4:57370358-57370377 None:intergenic 15.0%
!!! ATTTTAATGAAAATATATGC+CGG - chr5_4:57370358-57370377 Msa0859200:five_prime_UTR 15.0%
!!! ATCTTTCTTTTTTTTTCCAT+TGG - chr5_4:57368289-57368308 Msa0859200:CDS 20.0%
!! TTTCATTGAGAATATATTGA+TGG + chr5_4:57368323-57368342 None:intergenic 20.0%
!! TTTCTAATACTTTCTTCTTA+TGG - chr5_4:57368789-57368808 Msa0859200:CDS 20.0%
!! TATTGAAAATCTAAAGGAAA+AGG - chr5_4:57368874-57368893 Msa0859200:CDS 20.0%
!!! GAAATTTGAAATGGATTTTA+TGG - chr5_4:57369478-57369497 Msa0859200:intron 20.0%
!!! GAGTAACGTTTTTATATAAA+GGG + chr5_4:57369863-57369882 None:intergenic 20.0%
!!! TGAGTAACGTTTTTATATAA+AGG + chr5_4:57369864-57369883 None:intergenic 20.0%
!! ATATAAAAACGTTACTCATT+TGG - chr5_4:57369866-57369885 Msa0859200:intron 20.0%
!! TATAAAAACGTTACTCATTT+GGG - chr5_4:57369867-57369886 Msa0859200:intron 20.0%
!! TTATTATTAGGGTTAAGATT+AGG + chr5_4:57369966-57369985 None:intergenic 20.0%
!!! TTTATCAAGCTTTATTTTGT+TGG - chr5_4:57370277-57370296 Msa0859200:exon 20.0%
!!! ATCTTTCTTTTTTTTTCCAT+TGG - chr5_4:57368289-57368308 Msa0859200:CDS 20.0%
!! TTTCATTGAGAATATATTGA+TGG + chr5_4:57368323-57368342 None:intergenic 20.0%
!! TTTCTAATACTTTCTTCTTA+TGG - chr5_4:57368789-57368808 Msa0859200:CDS 20.0%
!! TATTGAAAATCTAAAGGAAA+AGG - chr5_4:57368874-57368893 Msa0859200:CDS 20.0%
!!! GAAATTTGAAATGGATTTTA+TGG - chr5_4:57369478-57369497 Msa0859200:intron 20.0%
!!! GAGTAACGTTTTTATATAAA+GGG + chr5_4:57369863-57369882 None:intergenic 20.0%
!!! TGAGTAACGTTTTTATATAA+AGG + chr5_4:57369864-57369883 None:intergenic 20.0%
!! ATATAAAAACGTTACTCATT+TGG - chr5_4:57369866-57369885 Msa0859200:intron 20.0%
!! TATAAAAACGTTACTCATTT+GGG - chr5_4:57369867-57369886 Msa0859200:intron 20.0%
!! TTATTATTAGGGTTAAGATT+AGG + chr5_4:57369966-57369985 None:intergenic 20.0%
!!! TTTATCAAGCTTTATTTTGT+TGG - chr5_4:57370277-57370296 Msa0859200:exon 20.0%
!! TCATAACTCTTCCTTTTAAA+TGG - chr5_4:57368092-57368111 Msa0859200:three_prime_UTR 25.0%
! GAAAGAGAAAACACAAAAAA+TGG - chr5_4:57368163-57368182 Msa0859200:three_prime_UTR 25.0%
! AAACACAAAAAATGGTTTCT+AGG - chr5_4:57368171-57368190 Msa0859200:three_prime_UTR 25.0%
! AACACAAAAAATGGTTTCTA+GGG - chr5_4:57368172-57368191 Msa0859200:three_prime_UTR 25.0%
!!! TTTGGGTCCATTTTTATTAT+TGG - chr5_4:57368399-57368418 Msa0859200:intron 25.0%
! GCAGAAAGAATGAATTAATA+AGG + chr5_4:57368510-57368529 None:intergenic 25.0%
!!! GTCTCTTTGAATTGTTTTAT+TGG + chr5_4:57369268-57369287 None:intergenic 25.0%
!!! TTTTTTAGGATAGATAGACA+AGG + chr5_4:57369294-57369313 None:intergenic 25.0%
! GAGTAATTGTTTACAATAAC+TGG + chr5_4:57369384-57369403 None:intergenic 25.0%
! TGCAAGTATGAAATTTGAAA+TGG - chr5_4:57369469-57369488 Msa0859200:intron 25.0%
!!! TTTGAAATGGATTTTATGGA+TGG - chr5_4:57369482-57369501 Msa0859200:intron 25.0%
!! TATAAGAAATCCCTTTTCTA+GGG + chr5_4:57369531-57369550 None:intergenic 25.0%
!! TTATAAGAAATCCCTTTTCT+AGG + chr5_4:57369532-57369551 None:intergenic 25.0%
! ACAAAAATATTGACAAGTGA+AGG + chr5_4:57369826-57369845 None:intergenic 25.0%
! ATAAAAACGTTACTCATTTG+GGG - chr5_4:57369868-57369887 Msa0859200:intron 25.0%
! GTACATTGAATCATTGATAT+TGG - chr5_4:57369917-57369936 Msa0859200:intron 25.0%
! TCTTCATTTAATTGTCTACT+AGG - chr5_4:57369993-57370012 Msa0859200:intron 25.0%
!!! AGCTTTATTTTGTTGGTTTA+AGG - chr5_4:57370284-57370303 Msa0859200:five_prime_UTR 25.0%
!! TCATAACTCTTCCTTTTAAA+TGG - chr5_4:57368092-57368111 Msa0859200:three_prime_UTR 25.0%
! GAAAGAGAAAACACAAAAAA+TGG - chr5_4:57368163-57368182 Msa0859200:three_prime_UTR 25.0%
! AAACACAAAAAATGGTTTCT+AGG - chr5_4:57368171-57368190 Msa0859200:three_prime_UTR 25.0%
! AACACAAAAAATGGTTTCTA+GGG - chr5_4:57368172-57368191 Msa0859200:three_prime_UTR 25.0%
!!! TTTGGGTCCATTTTTATTAT+TGG - chr5_4:57368399-57368418 Msa0859200:intron 25.0%
! GCAGAAAGAATGAATTAATA+AGG + chr5_4:57368510-57368529 None:intergenic 25.0%
!!! GTCTCTTTGAATTGTTTTAT+TGG + chr5_4:57369268-57369287 None:intergenic 25.0%
!!! TTTTTTAGGATAGATAGACA+AGG + chr5_4:57369294-57369313 None:intergenic 25.0%
! GAGTAATTGTTTACAATAAC+TGG + chr5_4:57369384-57369403 None:intergenic 25.0%
! TGCAAGTATGAAATTTGAAA+TGG - chr5_4:57369469-57369488 Msa0859200:intron 25.0%
!!! TTTGAAATGGATTTTATGGA+TGG - chr5_4:57369482-57369501 Msa0859200:intron 25.0%
!! TATAAGAAATCCCTTTTCTA+GGG + chr5_4:57369531-57369550 None:intergenic 25.0%
!! TTATAAGAAATCCCTTTTCT+AGG + chr5_4:57369532-57369551 None:intergenic 25.0%
! ACAAAAATATTGACAAGTGA+AGG + chr5_4:57369826-57369845 None:intergenic 25.0%
! ATAAAAACGTTACTCATTTG+GGG - chr5_4:57369868-57369887 Msa0859200:intron 25.0%
! GTACATTGAATCATTGATAT+TGG - chr5_4:57369917-57369936 Msa0859200:intron 25.0%
! TCTTCATTTAATTGTCTACT+AGG - chr5_4:57369993-57370012 Msa0859200:intron 25.0%
!!! AGCTTTATTTTGTTGGTTTA+AGG - chr5_4:57370284-57370303 Msa0859200:five_prime_UTR 25.0%
GATAAGAGATAGAAAATGCA+GGG + chr5_4:57368272-57368291 None:intergenic 30.0%
AGATAAGAGATAGAAAATGC+AGG + chr5_4:57368273-57368292 None:intergenic 30.0%
TATACTCTTGTTCCTGATTT+GGG - chr5_4:57368382-57368401 Msa0859200:intron 30.0%
AATAAAAATGGACCCAAATC+AGG + chr5_4:57368397-57368416 None:intergenic 30.0%
AGGATACACATAAACTACTA+TGG + chr5_4:57368714-57368733 None:intergenic 30.0%
GCTGATAAGAATATAGGATA+AGG + chr5_4:57368734-57368753 None:intergenic 30.0%
CACATAGCTGATAAGAATAT+AGG + chr5_4:57368740-57368759 None:intergenic 30.0%
CTATATTCTTATCAGCTATG+TGG - chr5_4:57368738-57368757 Msa0859200:intron 30.0%
TATGTGGAGCTTATTTGATA+TGG - chr5_4:57368754-57368773 Msa0859200:intron 30.0%
! CTTCTTATGGTTTTTATGCA+TGG - chr5_4:57368802-57368821 Msa0859200:CDS 30.0%
! TATGGTTTTTATGCATGGTT+TGG - chr5_4:57368807-57368826 Msa0859200:CDS 30.0%
! GTACTTTTCTCAATTACCAT+AGG + chr5_4:57368962-57368981 None:intergenic 30.0%
!! CTGCTTTTGGATAAACAATT+TGG - chr5_4:57369058-57369077 Msa0859200:intron 30.0%
!!! TAGGGTCAAGACTTTTTTTT+AGG + chr5_4:57369308-57369327 None:intergenic 30.0%
ATTCTGCTGCAAGTTATTTA+GGG + chr5_4:57369326-57369345 None:intergenic 30.0%
AATTCTGCTGCAAGTTATTT+AGG + chr5_4:57369327-57369346 None:intergenic 30.0%
!! AATTTTTGTTTCCAAGCTGT+TGG - chr5_4:57369656-57369675 Msa0859200:intron 30.0%
AAGATTAGGCACCATATATA+TGG + chr5_4:57369952-57369971 None:intergenic 30.0%
CTTATGTAGAATGAAGAACA+AGG - chr5_4:57370106-57370125 Msa0859200:intron 30.0%
!!! ATTTTTTATTGCTTGTCCCA+CGG + chr5_4:57370164-57370183 None:intergenic 30.0%
TGGGACAAGCAATAAAAAAT+TGG - chr5_4:57370164-57370183 Msa0859200:intron 30.0%
AAAATATATGCCGGTAGTAA+GGG - chr5_4:57370367-57370386 Msa0859200:five_prime_UTR 30.0%
TTCAAATCCATATGATCTCA+CGG + chr5_4:57368069-57368088 None:intergenic 30.0%
GATAAGAGATAGAAAATGCA+GGG + chr5_4:57368272-57368291 None:intergenic 30.0%
AGATAAGAGATAGAAAATGC+AGG + chr5_4:57368273-57368292 None:intergenic 30.0%
TATACTCTTGTTCCTGATTT+GGG - chr5_4:57368382-57368401 Msa0859200:intron 30.0%
AATAAAAATGGACCCAAATC+AGG + chr5_4:57368397-57368416 None:intergenic 30.0%
AGGATACACATAAACTACTA+TGG + chr5_4:57368714-57368733 None:intergenic 30.0%
GCTGATAAGAATATAGGATA+AGG + chr5_4:57368734-57368753 None:intergenic 30.0%
CACATAGCTGATAAGAATAT+AGG + chr5_4:57368740-57368759 None:intergenic 30.0%
CTATATTCTTATCAGCTATG+TGG - chr5_4:57368738-57368757 Msa0859200:intron 30.0%
TATGTGGAGCTTATTTGATA+TGG - chr5_4:57368754-57368773 Msa0859200:intron 30.0%
! CTTCTTATGGTTTTTATGCA+TGG - chr5_4:57368802-57368821 Msa0859200:CDS 30.0%
! TATGGTTTTTATGCATGGTT+TGG - chr5_4:57368807-57368826 Msa0859200:CDS 30.0%
! GTACTTTTCTCAATTACCAT+AGG + chr5_4:57368962-57368981 None:intergenic 30.0%
!! CTGCTTTTGGATAAACAATT+TGG - chr5_4:57369058-57369077 Msa0859200:intron 30.0%
!!! TAGGGTCAAGACTTTTTTTT+AGG + chr5_4:57369308-57369327 None:intergenic 30.0%
ATTCTGCTGCAAGTTATTTA+GGG + chr5_4:57369326-57369345 None:intergenic 30.0%
AATTCTGCTGCAAGTTATTT+AGG + chr5_4:57369327-57369346 None:intergenic 30.0%
!! AATTTTTGTTTCCAAGCTGT+TGG - chr5_4:57369656-57369675 Msa0859200:intron 30.0%
AAGATTAGGCACCATATATA+TGG + chr5_4:57369952-57369971 None:intergenic 30.0%
CTTATGTAGAATGAAGAACA+AGG - chr5_4:57370106-57370125 Msa0859200:intron 30.0%
!!! ATTTTTTATTGCTTGTCCCA+CGG + chr5_4:57370164-57370183 None:intergenic 30.0%
TGGGACAAGCAATAAAAAAT+TGG - chr5_4:57370164-57370183 Msa0859200:intron 30.0%
AAAATATATGCCGGTAGTAA+GGG - chr5_4:57370367-57370386 Msa0859200:five_prime_UTR 30.0%
ATTGATGGAGAGAAATCCAA+TGG + chr5_4:57368308-57368327 None:intergenic 35.0%
CTATACTCTTGTTCCTGATT+TGG - chr5_4:57368381-57368400 Msa0859200:intron 35.0%
! GGATGAGAGCATAAAAGTAT+AGG + chr5_4:57368544-57368563 None:intergenic 35.0%
TTTGAACCTAGATATGCATG+TGG + chr5_4:57368565-57368584 None:intergenic 35.0%
TCAATTACCATAGGCAAGTT+GGG + chr5_4:57368953-57368972 None:intergenic 35.0%
! GATCAGAATTTGACTGCTTT+TGG - chr5_4:57369045-57369064 Msa0859200:intron 35.0%
TTGTTAATATCGCGAGCTAA+TGG + chr5_4:57369211-57369230 None:intergenic 35.0%
CTCACAAGTGTAATGTTAGT+AGG - chr5_4:57369232-57369251 Msa0859200:intron 35.0%
TTAGGATAGATAGACAAGGA+TGG + chr5_4:57369290-57369309 None:intergenic 35.0%
GTATTGAAACAAGCATACCT+TGG + chr5_4:57369577-57369596 None:intergenic 35.0%
TGATTCAATGTACAGCAACT+CGG + chr5_4:57369910-57369929 None:intergenic 35.0%
GGTCATGCAATCCATATATA+TGG - chr5_4:57369938-57369957 Msa0859200:intron 35.0%
TCGTTATTGATGAACTTAGC+TGG + chr5_4:57370027-57370046 None:intergenic 35.0%
!! AGAATGACGGAAAGTTTTTG+TGG - chr5_4:57370083-57370102 Msa0859200:intron 35.0%
GGTTGCAATTTACACTTGAT+CGG + chr5_4:57370200-57370219 None:intergenic 35.0%
ATTGGCAGAACTTGAAGATT+TGG + chr5_4:57370340-57370359 None:intergenic 35.0%
GAAAATATATGCCGGTAGTA+AGG - chr5_4:57370366-57370385 Msa0859200:five_prime_UTR 35.0%
ATTGATGGAGAGAAATCCAA+TGG + chr5_4:57368308-57368327 None:intergenic 35.0%
CTATACTCTTGTTCCTGATT+TGG - chr5_4:57368381-57368400 Msa0859200:intron 35.0%
! GGATGAGAGCATAAAAGTAT+AGG + chr5_4:57368544-57368563 None:intergenic 35.0%
TTTGAACCTAGATATGCATG+TGG + chr5_4:57368565-57368584 None:intergenic 35.0%
TCAATTACCATAGGCAAGTT+GGG + chr5_4:57368953-57368972 None:intergenic 35.0%
! GATCAGAATTTGACTGCTTT+TGG - chr5_4:57369045-57369064 Msa0859200:intron 35.0%
TTGTTAATATCGCGAGCTAA+TGG + chr5_4:57369211-57369230 None:intergenic 35.0%
CTCACAAGTGTAATGTTAGT+AGG - chr5_4:57369232-57369251 Msa0859200:intron 35.0%
TTAGGATAGATAGACAAGGA+TGG + chr5_4:57369290-57369309 None:intergenic 35.0%
GTATTGAAACAAGCATACCT+TGG + chr5_4:57369577-57369596 None:intergenic 35.0%
TGATTCAATGTACAGCAACT+CGG + chr5_4:57369910-57369929 None:intergenic 35.0%
GGTCATGCAATCCATATATA+TGG - chr5_4:57369938-57369957 Msa0859200:intron 35.0%
TCGTTATTGATGAACTTAGC+TGG + chr5_4:57370027-57370046 None:intergenic 35.0%
!! AGAATGACGGAAAGTTTTTG+TGG - chr5_4:57370083-57370102 Msa0859200:intron 35.0%
GGTTGCAATTTACACTTGAT+CGG + chr5_4:57370200-57370219 None:intergenic 35.0%
ATTGGCAGAACTTGAAGATT+TGG + chr5_4:57370340-57370359 None:intergenic 35.0%
GAAAATATATGCCGGTAGTA+AGG - chr5_4:57370366-57370385 Msa0859200:five_prime_UTR 35.0%
!! GAGTTCTGAGGCCATTTAAA+AGG + chr5_4:57368106-57368125 None:intergenic 40.0%
ACCAATTCTGATGAGTTCTG+AGG + chr5_4:57368118-57368137 None:intergenic 40.0%
! GAGCATAAAAGTATAGGCAG+TGG + chr5_4:57368538-57368557 None:intergenic 40.0%
TCTCATCCACATGCATATCT+AGG - chr5_4:57368556-57368575 Msa0859200:intron 40.0%
CTCAATTACCATAGGCAAGT+TGG + chr5_4:57368954-57368973 None:intergenic 40.0%
TTCGTTAACCGTCTTGATGA+AGG - chr5_4:57369130-57369149 Msa0859200:intron 40.0%
TCGTTAACCGTCTTGATGAA+GGG - chr5_4:57369131-57369150 Msa0859200:intron 40.0%
TCATAAGCCCTTCATCAAGA+CGG + chr5_4:57369141-57369160 None:intergenic 40.0%
!! TTTTATGGATGGACTTGTGC+AGG - chr5_4:57369493-57369512 Msa0859200:intron 40.0%
TACAGTGGAAACCCTAGAAA+AGG - chr5_4:57369517-57369536 Msa0859200:CDS 40.0%
ACAGTGGAAACCCTAGAAAA+GGG - chr5_4:57369518-57369537 Msa0859200:CDS 40.0%
! TTTTCTGCAAAAAGCAGCCA+AGG - chr5_4:57369557-57369576 Msa0859200:CDS 40.0%
TTGAAGCCATTGAAGAGATG+AGG - chr5_4:57369597-57369616 Msa0859200:CDS 40.0%
AGTAAGCCTCATCTCTTCAA+TGG + chr5_4:57369606-57369625 None:intergenic 40.0%
!! TTTTGTTTCCAAGCTGTTGG+AGG - chr5_4:57369659-57369678 Msa0859200:intron 40.0%
! ATTTGGGGTTCTCGAGAAAA+GGG - chr5_4:57369883-57369902 Msa0859200:intron 40.0%
!! GAGTTCTGAGGCCATTTAAA+AGG + chr5_4:57368106-57368125 None:intergenic 40.0%
ACCAATTCTGATGAGTTCTG+AGG + chr5_4:57368118-57368137 None:intergenic 40.0%
! GAGCATAAAAGTATAGGCAG+TGG + chr5_4:57368538-57368557 None:intergenic 40.0%
TCTCATCCACATGCATATCT+AGG - chr5_4:57368556-57368575 Msa0859200:intron 40.0%
CTCAATTACCATAGGCAAGT+TGG + chr5_4:57368954-57368973 None:intergenic 40.0%
TTCGTTAACCGTCTTGATGA+AGG - chr5_4:57369130-57369149 Msa0859200:intron 40.0%
TCGTTAACCGTCTTGATGAA+GGG - chr5_4:57369131-57369150 Msa0859200:intron 40.0%
TCATAAGCCCTTCATCAAGA+CGG + chr5_4:57369141-57369160 None:intergenic 40.0%
!! TTTTATGGATGGACTTGTGC+AGG - chr5_4:57369493-57369512 Msa0859200:intron 40.0%
TACAGTGGAAACCCTAGAAA+AGG - chr5_4:57369517-57369536 Msa0859200:CDS 40.0%
ACAGTGGAAACCCTAGAAAA+GGG - chr5_4:57369518-57369537 Msa0859200:CDS 40.0%
! TTTTCTGCAAAAAGCAGCCA+AGG - chr5_4:57369557-57369576 Msa0859200:CDS 40.0%
TTGAAGCCATTGAAGAGATG+AGG - chr5_4:57369597-57369616 Msa0859200:CDS 40.0%
AGTAAGCCTCATCTCTTCAA+TGG + chr5_4:57369606-57369625 None:intergenic 40.0%
!! TTTTGTTTCCAAGCTGTTGG+AGG - chr5_4:57369659-57369678 Msa0859200:intron 40.0%
! ATTTGGGGTTCTCGAGAAAA+GGG - chr5_4:57369883-57369902 Msa0859200:intron 40.0%
GCCTCAGAACTCATCAGAAT+TGG - chr5_4:57368114-57368133 Msa0859200:three_prime_UTR 45.0%
!! TGATGCATCGATTACGATCG+AGG + chr5_4:57368849-57368868 None:intergenic 45.0%
! CATTTGGGGTTCTCGAGAAA+AGG - chr5_4:57369882-57369901 Msa0859200:intron 45.0%
CCGTCATTCTCATGCATGAT+GGG + chr5_4:57370073-57370092 None:intergenic 45.0%
CCCATCATGCATGAGAATGA+CGG - chr5_4:57370070-57370089 Msa0859200:intron 45.0%
TCCGTCATTCTCATGCATGA+TGG + chr5_4:57370074-57370093 None:intergenic 45.0%
GCCTCAGAACTCATCAGAAT+TGG - chr5_4:57368114-57368133 Msa0859200:three_prime_UTR 45.0%
!! TGATGCATCGATTACGATCG+AGG + chr5_4:57368849-57368868 None:intergenic 45.0%
! CATTTGGGGTTCTCGAGAAA+AGG - chr5_4:57369882-57369901 Msa0859200:intron 45.0%
CCGTCATTCTCATGCATGAT+GGG + chr5_4:57370073-57370092 None:intergenic 45.0%
CCCATCATGCATGAGAATGA+CGG - chr5_4:57370070-57370089 Msa0859200:intron 45.0%
TCCGTCATTCTCATGCATGA+TGG + chr5_4:57370074-57370093 None:intergenic 45.0%
!! CTATAAAAAATTATATAAAT+GGG + chr5_4:57368990-57369009 None:intergenic 5.0%
!! TCTATAAAAAATTATATAAA+TGG + chr5_4:57368991-57369010 None:intergenic 5.0%
!! AAGAAAAATAATAATAAATA+TGG + chr5_4:57370250-57370269 None:intergenic 5.0%
!! CTATAAAAAATTATATAAAT+GGG + chr5_4:57368990-57369009 None:intergenic 5.0%
!! TCTATAAAAAATTATATAAA+TGG + chr5_4:57368991-57369010 None:intergenic 5.0%
!! AAGAAAAATAATAATAAATA+TGG + chr5_4:57370250-57370269 None:intergenic 5.0%
! CCATAGGCAAGTTGGGATTG+TGG + chr5_4:57368946-57368965 None:intergenic 50.0%
CCACAATCCCAACTTGCCTA+TGG - chr5_4:57368943-57368962 Msa0859200:intron 50.0%
TGGACTTGTGCAGGTTACAG+TGG - chr5_4:57369502-57369521 Msa0859200:CDS 50.0%
AGAGATGAGGCTTACTGTGC+TGG - chr5_4:57369610-57369629 Msa0859200:intron 50.0%
GCTTACTGTGCTGGAAGCTA+GGG - chr5_4:57369619-57369638 Msa0859200:intron 50.0%
TTTCCAAGCTGTTGGAGGAG+AGG - chr5_4:57369664-57369683 Msa0859200:intron 50.0%
TTACCTCTCCTCCAACAGCT+TGG + chr5_4:57369670-57369689 None:intergenic 50.0%
ACAAGCTGTGAAACAAGCCG+TGG - chr5_4:57370144-57370163 Msa0859200:intron 50.0%
CAAGCTGTGAAACAAGCCGT+GGG - chr5_4:57370145-57370164 Msa0859200:intron 50.0%
! CCATAGGCAAGTTGGGATTG+TGG + chr5_4:57368946-57368965 None:intergenic 50.0%
CCACAATCCCAACTTGCCTA+TGG - chr5_4:57368943-57368962 Msa0859200:intron 50.0%
TGGACTTGTGCAGGTTACAG+TGG - chr5_4:57369502-57369521 Msa0859200:CDS 50.0%
AGAGATGAGGCTTACTGTGC+TGG - chr5_4:57369610-57369629 Msa0859200:intron 50.0%
GCTTACTGTGCTGGAAGCTA+GGG - chr5_4:57369619-57369638 Msa0859200:intron 50.0%
TTTCCAAGCTGTTGGAGGAG+AGG - chr5_4:57369664-57369683 Msa0859200:intron 50.0%
TTACCTCTCCTCCAACAGCT+TGG + chr5_4:57369670-57369689 None:intergenic 50.0%
ACAAGCTGTGAAACAAGCCG+TGG - chr5_4:57370144-57370163 Msa0859200:intron 50.0%
CAAGCTGTGAAACAAGCCGT+GGG - chr5_4:57370145-57370164 Msa0859200:intron 50.0%
GGCTTACTGTGCTGGAAGCT+AGG - chr5_4:57369618-57369637 Msa0859200:intron 55.0%
GGCTTACTGTGCTGGAAGCT+AGG - chr5_4:57369618-57369637 Msa0859200:intron 55.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 57368069 57370393 57368069 ID=Msa0859200;Name=Msa0859200
chr5_4 mRNA 57368069 57370393 57368069 ID=Msa0859200-mRNA-1;Parent=Msa0859200;Name=Msa0859200-mRNA-1;_AED=0.09;_eAED=0.09;_QI=113|1|1|1|1|1|4|183|157
chr5_4 exon 57368069 57368347 57368069 ID=Msa0859200-mRNA-1:exon:17497;Parent=Msa0859200-mRNA-1
chr5_4 exon 57368777 57368947 57368777 ID=Msa0859200-mRNA-1:exon:17496;Parent=Msa0859200-mRNA-1
chr5_4 exon 57369498 57369626 57369498 ID=Msa0859200-mRNA-1:exon:17495;Parent=Msa0859200-mRNA-1
chr5_4 exon 57370203 57370393 57370203 ID=Msa0859200-mRNA-1:exon:17494;Parent=Msa0859200-mRNA-1
chr5_4 five_prime_UTR 57370281 57370393 57370281 ID=Msa0859200-mRNA-1:five_prime_utr;Parent=Msa0859200-mRNA-1
chr5_4 CDS 57370203 57370280 57370203 ID=Msa0859200-mRNA-1:cds;Parent=Msa0859200-mRNA-1
chr5_4 CDS 57369498 57369626 57369498 ID=Msa0859200-mRNA-1:cds;Parent=Msa0859200-mRNA-1
chr5_4 CDS 57368777 57368947 57368777 ID=Msa0859200-mRNA-1:cds;Parent=Msa0859200-mRNA-1
chr5_4 CDS 57368252 57368347 57368252 ID=Msa0859200-mRNA-1:cds;Parent=Msa0859200-mRNA-1
chr5_4 three_prime_UTR 57368069 57368251 57368069 ID=Msa0859200-mRNA-1:three_prime_utr;Parent=Msa0859200-mRNA-1
Gene Sequence

>Msa0859200

ATGGTTTCTAGGGAGCAAAAGAGAGCAGCACTGCATGAGAAGCTGCAACTTCTTCGTTCTGTTACTAACTCTCATGCTCTAAATGATACCTCGATCGTAATCGATGCATCAAAGTATATTGAAAATCTAAAGGAAAAGGTAGAACGACTGAATGAAGAAATAGCTTCTGCAGAAAGTTCAAGTGTCCACAATCCCAACTTGCCTATGGTTACAGTGGAAACCCTAGAAAAGGGATTTCTTATAAACGTTTTTTCTGCAAAAAGCAGCCAAGGTATGCTTGTTTCAATACTTGAAGCCATTGAAGAGATGAGGCTTACTGTGCTGGAAGCTAGGGTTTCTTGTACAGACAATTTTTGTTTCCAAGCTGTTGGAGGAGAGAATGAAGAACAAGGTGAGACTATTGATGCACAAGCTGTGAAACAAGCCGTGGGACAAGCAATAAAAAATTGGAGTGAAAGTTCCGATCAAGTGTAA
Protein sequence

>Msa0859200

MVSREQKRAALHEKLQLLRSVTNSHALNDTSIVIDASKYIENLKEKVERLNEEIASAESSSVHNPNLPMVTVETLEKGFLINVFSAKSSQGMLVSILEAIEEMRLTVLEARVSCTDNFCFQAVGGENEEQGETIDAQAVKQAVGQAIKNWSESSDQV*