AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0819080


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0819080 MtrunA17_Chr5g0437991 97.765 179 4 0 1 179 1 179 2.39e-127 355
Msa0819080 MtrunA17_Chr3g0106861 46.835 158 82 1 25 180 1 158 1.29e-45 147
Msa0819080 MtrunA17_Chr7g0244441 58.537 123 50 1 51 173 6 127 5.39e-43 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0819080 AT2G40435.1 64.103 156 54 2 25 179 1 155 1.32e-67 203
Msa0819080 AT3G56220.1 57.419 155 63 3 25 177 1 154 5.06e-57 176
Msa0819080 AT2G40435.2 65.354 127 43 1 25 150 1 127 1.45e-55 172
Msa0819080 AT2G40435.3 64.341 129 45 1 25 152 1 129 1.57e-55 172
Msa0819080 AT3G56220.2 58.015 131 48 4 25 151 1 128 1.54e-43 142

Find 31 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGTTCTGAGGCCATTTAAA+AGG 0.297363 5_3:+55161537 None:intergenic
ATTGGCAGAACTTGAAGATT+TGG 0.300801 5_3:+55159081 None:intergenic
GAAAATATATGCCGGTAGTA+AGG 0.350569 5_3:-55159052 Msa0819080:three_prime_UTR
CTCAATTACCATAGGCAAGT+TGG 0.394555 5_3:+55160689 None:intergenic
CAATACATATAAATAGATAT+TGG 0.395990 5_3:+55159200 None:intergenic
AGAAAACACATGAGATCATA+TGG 0.396404 5_3:-55161581 None:intergenic
GCCTCAGAACTCATCAGAAT+TGG 0.404408 5_3:-55161526 Msa0819080:CDS
TCAATTACCATAGGCAAGTT+GGG 0.413845 5_3:+55160690 None:intergenic
TACAGTGGAAACCCTAGAAA+AGG 0.442701 5_3:-55159918 Msa0819080:CDS
GCTTACTGTGCTGGAAGCTA+GGG 0.457026 5_3:-55159816 Msa0819080:CDS
GGTTGCAATTTACACTTGAT+CGG 0.468244 5_3:+55159221 None:intergenic
TATTGAAAATCTAAAGGAAA+AGG 0.484456 5_3:-55160766 Msa0819080:CDS
AGAGATGAGGCTTACTGTGC+TGG 0.485736 5_3:-55159825 Msa0819080:CDS
GGCTTACTGTGCTGGAAGCT+AGG 0.491888 5_3:-55159817 Msa0819080:CDS
CCACAATCCCAACTTGCCTA+TGG 0.504234 5_3:-55160697 Msa0819080:intron
AAAGTATATTGAAAATCTAA+AGG 0.516255 5_3:-55160772 Msa0819080:CDS
AGTAAGCCTCATCTCTTCAA+TGG 0.521158 5_3:+55159832 None:intergenic
CTTCCAAGCTGTTGGAGGAG+AGG 0.526484 5_3:-55159771 Msa0819080:intron
CCATAGGCAAGTTGGGATTG+TGG 0.527764 5_3:+55160697 None:intergenic
ACAGTGGAAACCCTAGAAAA+GGG 0.541907 5_3:-55159917 Msa0819080:CDS
AAAATATATGCCGGTAGTAA+GGG 0.546912 5_3:-55159051 Msa0819080:three_prime_UTR
TTACCTCTCCTCCAACAGCT+TGG 0.552308 5_3:+55159768 None:intergenic
AACAAACGGACCCTTACTAC+CGG 0.570419 5_3:+55159041 None:intergenic
TTGAAGCCATTGAAGAGATG+AGG 0.583640 5_3:-55159838 Msa0819080:CDS
TGATGCATCGATTACGATCG+AGG 0.609202 5_3:+55160794 None:intergenic
GTATTGAAACAAGCATACCT+TGG 0.629414 5_3:+55159861 None:intergenic
ACCAATTCTGATGAGTTCTG+AGG 0.646466 5_3:+55161525 None:intergenic
TGGACTTGTGCAGGTTACAG+TGG 0.658893 5_3:-55159933 Msa0819080:intron
ACAAGCTGTGAAACAAGCCG+TGG 0.676305 5_3:-55159274 Msa0819080:CDS
CAAGCTGTGAAACAAGCCGT+GGG 0.688309 5_3:-55159273 Msa0819080:CDS
CTTATGTAGAATGAAGAACA+AGG 0.699994 5_3:-55159312 Msa0819080:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATTCCAATAATAAAAA+TGG + chr5_3:55159387-55159406 None:intergenic 10.0%
!! AAGACTATATATTATTATTA+GGG + chr5_3:55161179-55161198 None:intergenic 10.0%
!!! GAATATTCTTTTATAGAATT+GGG + chr5_3:55159327-55159346 None:intergenic 15.0%
!!! TGAATATTCTTTTATAGAAT+TGG + chr5_3:55159328-55159347 None:intergenic 15.0%
!! TAATAAAAAAATAGCAAACT+TGG + chr5_3:55159414-55159433 None:intergenic 15.0%
!! AAAGTATATTGAAAATCTAA+AGG - chr5_3:55159846-55159865 Msa0819080:CDS 15.0%
!!! TATAGAGCAAATATTTTTTA+TGG - chr5_3:55159983-55160002 Msa0819080:intron 15.0%
!! AACTTATAAACAATTAAACA+TGG - chr5_3:55160361-55160380 Msa0819080:intron 15.0%
!!! TAATTCACTTTTTTTTTTCA+TGG - chr5_3:55160531-55160550 Msa0819080:intron 15.0%
!!! AATTCACTTTTTTTTTTCAT+GGG - chr5_3:55160532-55160551 Msa0819080:intron 15.0%
!! GAAGACTATATATTATTATT+AGG + chr5_3:55161180-55161199 None:intergenic 15.0%
!! CAATACATATAAATAGATAT+TGG + chr5_3:55161421-55161440 None:intergenic 15.0%
!!! GCATATATTTTCATTAAAAT+TGG + chr5_3:55161558-55161577 None:intergenic 15.0%
!!! ATTTTAATGAAAATATATGC+CGG - chr5_3:55161558-55161577 Msa0819080:five_prime_UTR 15.0%
!! AAAGAGAAAACACAAAAAAA+TGG - chr5_3:55159142-55159161 Msa0819080:three_prime_UTR 20.0%
!! TTTCATTGAGAATATATTGA+TGG + chr5_3:55159301-55159320 None:intergenic 20.0%
!! TTTCTAATACTTTCTTCTTA+TGG - chr5_3:55159767-55159786 Msa0819080:intron 20.0%
!! TATTGAAAATCTAAAGGAAA+AGG - chr5_3:55159852-55159871 Msa0819080:CDS 20.0%
!!! AGAATTTTTCAAGAAATAGT+TGG - chr5_3:55160037-55160056 Msa0819080:intron 20.0%
!!! AATAGTTGGCAAATTTTAAT+TGG - chr5_3:55160051-55160070 Msa0819080:intron 20.0%
!!! ATCTCTTTGAATTGTTTTAT+TGG + chr5_3:55160453-55160472 None:intergenic 20.0%
!!! GAAATTTGAAATGGATTTTA+TGG - chr5_3:55160661-55160680 Msa0819080:intron 20.0%
!!! GAGTAACGTTTTTATATAAA+GGG + chr5_3:55161065-55161084 None:intergenic 20.0%
!!! TGAGTAACGTTTTTATATAA+AGG + chr5_3:55161066-55161085 None:intergenic 20.0%
!! ATATAAAAACGTTACTCATT+TGG - chr5_3:55161068-55161087 Msa0819080:intron 20.0%
!! TATAAAAACGTTACTCATTT+GGG - chr5_3:55161069-55161088 Msa0819080:intron 20.0%
!! TTATTATTAGGGTTAAGATT+AGG + chr5_3:55161168-55161187 None:intergenic 20.0%
!!! TTTATCAAGCTTTATTTTGT+TGG - chr5_3:55161477-55161496 Msa0819080:CDS 20.0%
!! TCATAACTCTTCCTTTTAAA+TGG - chr5_3:55159070-55159089 Msa0819080:three_prime_UTR 25.0%
! AACACAAAAAAATGGTTTCT+AGG - chr5_3:55159150-55159169 Msa0819080:three_prime_UTR 25.0%
! ACACAAAAAAATGGTTTCTA+GGG - chr5_3:55159151-55159170 Msa0819080:three_prime_UTR 25.0%
! AATGGAAAGAAAAGAAAGAT+AGG + chr5_3:55159268-55159287 None:intergenic 25.0%
!! TATCTTTCTTTTCTTTCCAT+TGG - chr5_3:55159267-55159286 Msa0819080:CDS 25.0%
!!! TTTGGGTCCATTTTTATTAT+TGG - chr5_3:55159377-55159396 Msa0819080:intron 25.0%
! GCAGAAAGAATGAATTAATA+AGG + chr5_3:55159488-55159507 None:intergenic 25.0%
!!! TTTTTTAGGATAGATAGACA+AGG + chr5_3:55160479-55160498 None:intergenic 25.0%
! GAGAAATTGTTTACAATAAC+TGG + chr5_3:55160569-55160588 None:intergenic 25.0%
! TGCAAGTATGAAATTTGAAA+TGG - chr5_3:55160652-55160671 Msa0819080:intron 25.0%
!!! TTTGAAATGGATTTTATGGA+TGG - chr5_3:55160665-55160684 Msa0819080:intron 25.0%
!! TATAAGAAATCCCTTTTCTA+GGG + chr5_3:55160714-55160733 None:intergenic 25.0%
!! TTATAAGAAATCCCTTTTCT+AGG + chr5_3:55160715-55160734 None:intergenic 25.0%
! ACAAAAATATTGACAAGTGA+AGG + chr5_3:55161028-55161047 None:intergenic 25.0%
! ATAAAAACGTTACTCATTTG+GGG - chr5_3:55161070-55161089 Msa0819080:intron 25.0%
! GTACATTGAATCATTGATAT+TGG - chr5_3:55161119-55161138 Msa0819080:intron 25.0%
! TCTTCATTTAATTGTCTACT+AGG - chr5_3:55161195-55161214 Msa0819080:intron 25.0%
!!! AGCTTTATTTTGTTGGTTTA+AGG - chr5_3:55161484-55161503 Msa0819080:CDS 25.0%
TATACTCTTGTTCCTGATTT+GGG - chr5_3:55159360-55159379 Msa0819080:intron 30.0%
AATAAAAATGGACCCAAATC+AGG + chr5_3:55159375-55159394 None:intergenic 30.0%
GCTGATAAGAATATAGGATA+AGG + chr5_3:55159712-55159731 None:intergenic 30.0%
CACATAGCTGATAAGAATAT+AGG + chr5_3:55159718-55159737 None:intergenic 30.0%
CTATATTCTTATCAGCTATG+TGG - chr5_3:55159716-55159735 Msa0819080:intron 30.0%
TATGTGGAGCTTATTTGATA+TGG - chr5_3:55159732-55159751 Msa0819080:intron 30.0%
! CTTCTTATGGTTTTTATGCA+TGG - chr5_3:55159780-55159799 Msa0819080:CDS 30.0%
! TATGGTTTTTATGCATGGTT+TGG - chr5_3:55159785-55159804 Msa0819080:CDS 30.0%
! GTACTTTTCTCAATTACCAT+AGG + chr5_3:55159940-55159959 None:intergenic 30.0%
!! CTGCTTTTGGATAAACAATT+TGG - chr5_3:55160246-55160265 Msa0819080:intron 30.0%
!!! TAGGGTCAAGACTTTTTTTT+AGG + chr5_3:55160493-55160512 None:intergenic 30.0%
ATTCTGCTGCAAGTTATTTA+GGG + chr5_3:55160511-55160530 None:intergenic 30.0%
AATTCTGCTGCAAGTTATTT+AGG + chr5_3:55160512-55160531 None:intergenic 30.0%
AAGATTAGGCACCATATATA+TGG + chr5_3:55161154-55161173 None:intergenic 30.0%
CTTATGTAGAATGAAGAACA+AGG - chr5_3:55161306-55161325 Msa0819080:intron 30.0%
!!! ATTTTTTATTGCTTGTCCCA+CGG + chr5_3:55161364-55161383 None:intergenic 30.0%
TGGGACAAGCAATAAAAAAT+TGG - chr5_3:55161364-55161383 Msa0819080:intron 30.0%
AAAATATATGCCGGTAGTAA+GGG - chr5_3:55161567-55161586 Msa0819080:five_prime_UTR 30.0%
GATAGGAGATAGAAAATGCA+GGG + chr5_3:55159251-55159270 None:intergenic 35.0%
AGATAGGAGATAGAAAATGC+AGG + chr5_3:55159252-55159271 None:intergenic 35.0%
ATTGATGGAGAGAAATCCAA+TGG + chr5_3:55159286-55159305 None:intergenic 35.0%
CTATACTCTTGTTCCTGATT+TGG - chr5_3:55159359-55159378 Msa0819080:intron 35.0%
! GGATGAGAGCATAAAAGTAT+AGG + chr5_3:55159522-55159541 None:intergenic 35.0%
TTTGAACCTAGATATGCATG+TGG + chr5_3:55159543-55159562 None:intergenic 35.0%
AGGATACACATACACTACTA+TGG + chr5_3:55159692-55159711 None:intergenic 35.0%
TCAATTACCATAGGCAAGTT+GGG + chr5_3:55159931-55159950 None:intergenic 35.0%
! GATCAGAATTTGACTGCTTT+TGG - chr5_3:55160233-55160252 Msa0819080:intron 35.0%
! TTTTCGTTAACCGTCTTGAA+GGG - chr5_3:55160316-55160335 Msa0819080:intron 35.0%
! TTTTCATAAGCCCTTCAAGA+CGG + chr5_3:55160329-55160348 None:intergenic 35.0%
TTGTTAATATCGCGAGCTAA+TGG + chr5_3:55160396-55160415 None:intergenic 35.0%
CTCACAAGTGTAATGTTAGT+AGG - chr5_3:55160417-55160436 Msa0819080:intron 35.0%
GTATTGAAACAAGCATACCT+TGG + chr5_3:55160760-55160779 None:intergenic 35.0%
! AATTTTTGCTTCCAAGCTGT+TGG - chr5_3:55160839-55160858 Msa0819080:intron 35.0%
TGATTCAATGTACAGCAACT+CGG + chr5_3:55161112-55161131 None:intergenic 35.0%
GGTCATGCAATCCATATATA+TGG - chr5_3:55161140-55161159 Msa0819080:intron 35.0%
TCGTTATTGATGAACTTAGC+TGG + chr5_3:55161229-55161248 None:intergenic 35.0%
!! AGAATGACGGAAAGTTTTTG+TGG - chr5_3:55161283-55161302 Msa0819080:intron 35.0%
GGTTGCAATTTACACTTGAT+CGG + chr5_3:55161400-55161419 None:intergenic 35.0%
ATTGGCAGAACTTGAAGATT+TGG + chr5_3:55161540-55161559 None:intergenic 35.0%
GAAAATATATGCCGGTAGTA+AGG - chr5_3:55161566-55161585 Msa0819080:five_prime_UTR 35.0%
!! GAGTTCTGAGGCCATTTAAA+AGG + chr5_3:55159084-55159103 None:intergenic 40.0%
ACCAATTCTGATGAGTTCTG+AGG + chr5_3:55159096-55159115 None:intergenic 40.0%
! GAGCATAAAAGTATAGGCAG+TGG + chr5_3:55159516-55159535 None:intergenic 40.0%
TCTCATCCACATGCATATCT+AGG - chr5_3:55159534-55159553 Msa0819080:intron 40.0%
CTCAATTACCATAGGCAAGT+TGG + chr5_3:55159932-55159951 None:intergenic 40.0%
GTGGCTGAAAAATAGGAAGT+GGG + chr5_3:55160089-55160108 None:intergenic 40.0%
TGTGGCTGAAAAATAGGAAG+TGG + chr5_3:55160090-55160109 None:intergenic 40.0%
CTATTTGCCACAATTCAAGG+AGG + chr5_3:55160113-55160132 None:intergenic 40.0%
GCACTATTTGCCACAATTCA+AGG + chr5_3:55160116-55160135 None:intergenic 40.0%
GGCAAATAGTGCATTGTGAT+TGG - chr5_3:55160124-55160143 Msa0819080:intron 40.0%
! GTTTTCGTTAACCGTCTTGA+AGG - chr5_3:55160315-55160334 Msa0819080:intron 40.0%
!! TTTTATGGATGGACTTGTGC+AGG - chr5_3:55160676-55160695 Msa0819080:intron 40.0%
TACAGTGGAAACCCTAGAAA+AGG - chr5_3:55160700-55160719 Msa0819080:CDS 40.0%
ACAGTGGAAACCCTAGAAAA+GGG - chr5_3:55160701-55160720 Msa0819080:CDS 40.0%
! TTTTCTGCAAAAAGCAGCCA+AGG - chr5_3:55160740-55160759 Msa0819080:CDS 40.0%
TTGAAGCCATTGAAGAGATG+AGG - chr5_3:55160780-55160799 Msa0819080:CDS 40.0%
AGTAAGCCTCATCTCTTCAA+TGG + chr5_3:55160789-55160808 None:intergenic 40.0%
! ATTTGGGGTTCTCGAGAAAA+GGG - chr5_3:55161085-55161104 Msa0819080:intron 40.0%
GCCTCAGAACTCATCAGAAT+TGG - chr5_3:55159092-55159111 Msa0819080:three_prime_UTR 45.0%
!! TGATGCATCGATTACGATCG+AGG + chr5_3:55159827-55159846 None:intergenic 45.0%
! AGGAGGTGTGGCTGAAAAAT+AGG + chr5_3:55160096-55160115 None:intergenic 45.0%
! TTTTGCTTCCAAGCTGTTGG+AGG - chr5_3:55160842-55160861 Msa0819080:intron 45.0%
! CATTTGGGGTTCTCGAGAAA+AGG - chr5_3:55161084-55161103 Msa0819080:intron 45.0%
CCGTCATTCTCATGCATGAT+GGG + chr5_3:55161273-55161292 None:intergenic 45.0%
CCCATCATGCATGAGAATGA+CGG - chr5_3:55161270-55161289 Msa0819080:intron 45.0%
TCCGTCATTCTCATGCATGA+TGG + chr5_3:55161274-55161293 None:intergenic 45.0%
!! CTATAAAAAATTATATAAAT+GGG + chr5_3:55159968-55159987 None:intergenic 5.0%
!! TCTATAAAAAATTATATAAA+TGG + chr5_3:55159969-55159988 None:intergenic 5.0%
!! AAGAAAAATAATAATAAATA+TGG + chr5_3:55161450-55161469 None:intergenic 5.0%
! CCATAGGCAAGTTGGGATTG+TGG + chr5_3:55159924-55159943 None:intergenic 50.0%
CCACAATCCCAACTTGCCTA+TGG - chr5_3:55159921-55159940 Msa0819080:CDS 50.0%
AGCCACACCTCCTTGAATTG+TGG - chr5_3:55160103-55160122 Msa0819080:intron 50.0%
TGCCACAATTCAAGGAGGTG+TGG + chr5_3:55160108-55160127 None:intergenic 50.0%
TGGACTTGTGCAGGTTACAG+TGG - chr5_3:55160685-55160704 Msa0819080:intron 50.0%
AGAGATGAGGCTTACTGTGC+TGG - chr5_3:55160793-55160812 Msa0819080:CDS 50.0%
GCTTACTGTGCTGGAAGCTA+GGG - chr5_3:55160802-55160821 Msa0819080:CDS 50.0%
TTACCTCTCCTCCAACAGCT+TGG + chr5_3:55160853-55160872 None:intergenic 50.0%
ACAAGCTGTGAAACAAGCCG+TGG - chr5_3:55161344-55161363 Msa0819080:intron 50.0%
CAAGCTGTGAAACAAGCCGT+GGG - chr5_3:55161345-55161364 Msa0819080:intron 50.0%
GGCTTACTGTGCTGGAAGCT+AGG - chr5_3:55160801-55160820 Msa0819080:CDS 55.0%
CTTCCAAGCTGTTGGAGGAG+AGG - chr5_3:55160847-55160866 Msa0819080:intron 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 55159047 55161593 55159047 ID=Msa0819080;Name=Msa0819080
chr5_3 mRNA 55159047 55161593 55159047 ID=Msa0819080-mRNA-1;Parent=Msa0819080;Name=Msa0819080-mRNA-1;_AED=0.11;_eAED=0.11;_QI=42|1|1|1|1|1|4|183|181
chr5_3 exon 55159047 55159325 55159047 ID=Msa0819080-mRNA-1:exon:16745;Parent=Msa0819080-mRNA-1
chr5_3 exon 55159772 55159942 55159772 ID=Msa0819080-mRNA-1:exon:16744;Parent=Msa0819080-mRNA-1
chr5_3 exon 55160698 55160826 55160698 ID=Msa0819080-mRNA-1:exon:16743;Parent=Msa0819080-mRNA-1
chr5_3 exon 55161402 55161593 55161402 ID=Msa0819080-mRNA-1:exon:16742;Parent=Msa0819080-mRNA-1
chr5_3 five_prime_UTR 55161552 55161593 55161552 ID=Msa0819080-mRNA-1:five_prime_utr;Parent=Msa0819080-mRNA-1
chr5_3 CDS 55161402 55161551 55161402 ID=Msa0819080-mRNA-1:cds;Parent=Msa0819080-mRNA-1
chr5_3 CDS 55160698 55160826 55160698 ID=Msa0819080-mRNA-1:cds;Parent=Msa0819080-mRNA-1
chr5_3 CDS 55159772 55159942 55159772 ID=Msa0819080-mRNA-1:cds;Parent=Msa0819080-mRNA-1
chr5_3 CDS 55159230 55159325 55159230 ID=Msa0819080-mRNA-1:cds;Parent=Msa0819080-mRNA-1
chr5_3 three_prime_UTR 55159047 55159229 55159047 ID=Msa0819080-mRNA-1:three_prime_utr;Parent=Msa0819080-mRNA-1
Gene Sequence

>Msa0819080

ATGGCCTCAGAACTCATCAGAATTGGTAGCAAAAGAAAAAGAGAGAGATATAGAAAGAGAAAACACAAAAAAATGGTTTCTAGGGAGCAAAAGAGAGCAGCACTGCATGAGAAGCTGCAACTTCTTCGTTCTGTTACTAACTCTCATGCTCTAAATGATACCTCGATCGTAATCGATGCATCAAAGTATATTGAAAATCTAAAGGAAAAGGTAGAACGACTGAATGAAGAAATAGCTTCTGCAGAAAGTTCAAGTGTCCACAATCCCAACTTGCCTATGGTTACAGTGGAAACCCTAGAAAAGGGATTTCTTATAAACGTTTTTTCTGCAAAAAGCAGCCAAGGTATGCTTGTTTCAATACTTGAAGCCATTGAAGAGATGAGGCTTACTGTGCTGGAAGCTAGGGTTTCTTGTACAGACAATTTTTGCTTCCAAGCTGTTGGAGGAGAGAATGAAGAACAAGGTGAGACTATTGATGCACAAGCTGTGAAACAAGCCGTGGGACAAGCAATAAAAAATTGGAGTGAAAGTTCCGATCAAGTGTAA

Protein sequence

>Msa0819080

MASELIRIGSKRKRERYRKRKHKKMVSREQKRAALHEKLQLLRSVTNSHALNDTSIVIDASKYIENLKEKVERLNEEIASAESSSVHNPNLPMVTVETLEKGFLINVFSAKSSQGMLVSILEAIEEMRLTVLEARVSCTDNFCFQAVGGENEEQGETIDAQAVKQAVGQAIKNWSESSDQV*