AlfalfaGEDB | Msa0865370

M. sativa cultivar zhongmu-4 / Msa0865370

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0865370	XP_003618062.1	71.981	207	31	2	1	180	1	207	1.37e-81	251

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0865370	G7K0N6	71.981	207	31	2	1	180	1	207	6.57e-82	251

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0007280	Msa0865370	0.832717	8.059933e-56	-8.615850e-47
Msa0054250	Msa0865370	0.830518	2.806932e-55	-8.615850e-47
Msa0054260	Msa0865370	0.829245	5.734259e-55	-8.615850e-47
Msa0054310	Msa0865370	0.833925	4.029158e-56	-8.615850e-47
Msa0100070	Msa0865370	0.804630	1.970270e-49	-8.615850e-47
Msa0100080	Msa0865370	0.804477	2.119702e-49	-8.615850e-47
Msa0100090	Msa0865370	0.823800	1.139370e-53	-8.615850e-47
Msa0658990	Msa0865370	0.804195	2.427150e-49	-8.615850e-47
Msa0148720	Msa0865370	0.834548	2.812918e-56	-8.615850e-47
Msa0197660	Msa0865370	0.820391	7.031895e-53	-8.615850e-47
Msa0239800	Msa0865370	0.821496	3.914428e-53	-8.615850e-47
Msa0277820	Msa0865370	0.809166	2.164118e-50	-8.615850e-47
Msa0313920	Msa0865370	0.802595	5.207222e-49	-8.615850e-47
Msa0317510	Msa0865370	0.820584	6.351336e-53	-8.615850e-47
Msa0865370	Msa0865390	0.829729	4.373310e-55	-8.615850e-47
Msa0865370	Msa1002320	0.805508	1.290277e-49	-8.615850e-47
Msa0865370	Msa1006560	0.800930	1.143570e-48	-8.615850e-47
Msa0865370	Msa1006630	0.817976	2.493146e-52	-8.615850e-47
Msa0865370	Msa1027240	0.801924	7.158085e-49	-8.615850e-47
Msa0865370	Msa1110960	0.808041	3.764076e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0865370	MtrunA17_Chr5g0448291	71.981	207	31	2	1	180	1	207	1.26e-85	251
Msa0865370	MtrunA17_Chr5g0448311	50.901	222	42	5	1	164	1	213	4.36e-50	160
Msa0865370	MtrunA17_Chr5g0448321	43.617	188	73	6	13	178	10	186	4.05e-31	111

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 14 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGGGATTCACTTTCTATA+AGG	0.250035	5_4:-65997238	None:intergenic
AAATGAAGAAGTGAAGAGTT+TGG	0.384795	5_4:+65997268	Msa0865370:CDS
AGTGAAGAGTTTGGTGATAG+TGG	0.385158	5_4:+65997277	Msa0865370:CDS
GAGATTACAGAATCAATTGA+TGG	0.442182	5_4:+65997209	Msa0865370:CDS
TGAGAATGTGAGATTGAAAC+AGG	0.459687	5_4:-65996923	None:intergenic
TTGCATAAGGAATGGCTTCA+AGG	0.464946	5_4:-65997009	None:intergenic
GAGAACACAGAACAGAGAAA+AGG	0.479620	5_4:-65996958	None:intergenic
CTGATATGTAAAATTCGAAC+CGG	0.481168	5_4:-65997181	None:intergenic
TTCATAAGTGGTTAATGAAG+AGG	0.499381	5_4:-65997123	None:intergenic
CTAAAGAATTGCATAAGGAA+TGG	0.511448	5_4:-65997017	None:intergenic
TGTTGCTAAAGAATTGCATA+AGG	0.546812	5_4:-65997022	None:intergenic
TTCAAGGACTGATAGAGTAA+AGG	0.552897	5_4:-65996993	None:intergenic
TCGACAGTGAGAAGAGTCTC+CGG	0.579495	5_4:+65997162	Msa0865370:CDS
GGAGACTCTTCTCACTGTCG+AGG	0.654535	5_4:-65997160	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGTTGAATGTTTTCATAAG+TGG	-	chr5_4:65997138-65997157	None:intergenic	25.0%
!	GAAACTAAACAAAAAATGTG+AGG	+	chr5_4:65997337-65997356	Msa0865370:CDS	25.0%
	CTAAAGAATTGCATAAGGAA+TGG	-	chr5_4:65997020-65997039	None:intergenic	30.0%
	TGTTGCTAAAGAATTGCATA+AGG	-	chr5_4:65997025-65997044	None:intergenic	30.0%
	TTCATAAGTGGTTAATGAAG+AGG	-	chr5_4:65997126-65997145	None:intergenic	30.0%
	CTGATATGTAAAATTCGAAC+CGG	-	chr5_4:65997184-65997203	None:intergenic	30.0%
	GAGATTACAGAATCAATTGA+TGG	+	chr5_4:65997209-65997228	Msa0865370:CDS	30.0%
!	TTCTTCATTTTCTTCTGCTT+GGG	-	chr5_4:65997258-65997277	None:intergenic	30.0%
	AAATGAAGAAGTGAAGAGTT+TGG	+	chr5_4:65997268-65997287	Msa0865370:CDS	30.0%
	TGAGAATGTGAGATTGAAAC+AGG	-	chr5_4:65996926-65996945	None:intergenic	35.0%
	TTCAAGGACTGATAGAGTAA+AGG	-	chr5_4:65996996-65997015	None:intergenic	35.0%
	CTTGGGATTCACTTTCTATA+AGG	-	chr5_4:65997241-65997260	None:intergenic	35.0%
!	CTTCTTCATTTTCTTCTGCT+TGG	-	chr5_4:65997259-65997278	None:intergenic	35.0%
	GAGAACACAGAACAGAGAAA+AGG	-	chr5_4:65996961-65996980	None:intergenic	40.0%
	TTGCATAAGGAATGGCTTCA+AGG	-	chr5_4:65997012-65997031	None:intergenic	40.0%
!	AGTGAAGAGTTTGGTGATAG+TGG	+	chr5_4:65997277-65997296	Msa0865370:CDS	40.0%
	TCGACAGTGAGAAGAGTCTC+CGG	+	chr5_4:65997162-65997181	Msa0865370:CDS	50.0%
	GGAGACTCTTCTCACTGTCG+AGG	-	chr5_4:65997163-65997182	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	65996909	65997469	65996909	ID=Msa0865370;Name=Msa0865370
chr5_4	mRNA	65996909	65997469	65996909	ID=Msa0865370-mRNA-1;Parent=Msa0865370;Name=Msa0865370-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|186
chr5_4	exon	65996909	65997469	65996909	ID=Msa0865370-mRNA-1:exon:20884;Parent=Msa0865370-mRNA-1
chr5_4	CDS	65996909	65997469	65996909	ID=Msa0865370-mRNA-1:cds;Parent=Msa0865370-mRNA-1

Gene Sequence

>Msa0865370

ATGGATTCTTTCAACCTGTTTCAATCTCACATTCTCAAGTTTCTACTCTCCTTTTCTCTGTTCTGTGTTCTCTTTTCTTTACTTCCTTTACTCTATCAGTCCTTGAAGCCATTCCTTATGCAATTCTTTAGCAACATCATTGAAAAGACTTACATGTTTCTTCTGTGCAATACACTTCTTGCTTTTATAGCATTGTATTCTACTATAGTCAATACCTCTTCATTAACCACTTATGAAAACATTCAACATGTCCTCGACAGTGAGAAGAGTCTCCGGTTCGAATTTTACATATCAGAATCAGAGATTACAGAATCAATTGATGGTGATAACCTTATAGAAAGTGAATCCCAAGCAGAAGAAAATGAAGAAGTGAAGAGTTTGGTGATAGTGGAACAAGAAAGTGTGATTATTGATGAAGATGAGACTGAGAAACTAAACAAAAAATGTGAGGATTTTATCAAAAAAATGAAAGCTGCTTTCTGTTCTGATTCAAGAGCAGAGAATAAGTTTTATTTTGATTATGATCAGAAATCAGAATCAGAAATCACTTGTGCTTGTTAA

Protein sequence

>Msa0865370

MDSFNLFQSHILKFLLSFSLFCVLFSLLPLLYQSLKPFLMQFFSNIIEKTYMFLLCNTLLAFIALYSTIVNTSSLTTYENIQHVLDSEKSLRFEFYISESEITESIDGDNLIESESQAEENEEVKSLVIVEQESVIIDEDETEKLNKKCEDFIKKMKAAFCSDSRAENKFYFDYDQKSESEITCAC*