AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0658990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0658990 MtrunA17_Chr8g0383251 91.304 184 8 1 1 176 1 184 2.67e-122 342
Msa0658990 MtrunA17_Chr3g0129321 54.082 196 66 8 1 175 1 193 4.15e-55 173
Msa0658990 MtrunA17_Chr1g0150801 46.358 151 65 3 13 156 17 158 1.21e-36 124
Msa0658990 MtrunA17_Chr2g0294231 58.427 89 36 1 83 171 186 273 7.70e-31 115
Msa0658990 MtrunA17_Chr7g0218081 42.975 121 56 1 58 165 355 475 4.51e-29 111
Msa0658990 MtrunA17_Chr7g0218081 56.667 60 25 1 100 159 228 286 2.10e-14 70.1
Msa0658990 MtrunA17_Chr7g0270191 46.957 115 54 2 58 167 100 212 1.43e-28 107
Msa0658990 MtrunA17_Chr3g0101131 38.889 144 75 2 35 165 337 480 4.42e-28 109
Msa0658990 MtrunA17_Chr3g0101131 49.206 63 31 1 100 162 243 304 5.97e-11 60.1
Msa0658990 MtrunA17_Chr4g0051881 58.108 74 31 0 86 159 164 237 6.55e-28 106
Msa0658990 MtrunA17_Chr1g0194481 51.087 92 42 1 74 165 135 223 1.13e-27 105
Msa0658990 MtrunA17_Chr4g0004911 39.850 133 78 1 28 160 266 396 1.60e-27 107
Msa0658990 MtrunA17_Chr4g0004911 37.671 146 74 3 14 159 115 243 2.04e-17 79.0
Msa0658990 MtrunA17_Chr4g0042831 46.078 102 42 1 71 159 236 337 4.52e-27 105
Msa0658990 MtrunA17_Chr4g0042831 49.206 63 32 0 100 162 145 207 1.41e-12 64.7
Msa0658990 MtrunA17_Chr5g0443131 59.211 76 31 0 86 161 143 218 5.14e-27 103
Msa0658990 MtrunA17_Chr5g0445311 48.889 90 46 0 86 175 350 439 7.58e-27 105
Msa0658990 MtrunA17_Chr5g0445311 49.206 63 31 1 100 162 200 261 6.44e-11 60.1
Msa0658990 MtrunA17_Chr5g0418191 58.108 74 31 0 86 159 306 379 7.62e-27 105
Msa0658990 MtrunA17_Chr5g0418191 53.333 60 27 1 100 159 151 209 6.63e-13 65.9
Msa0658990 MtrunA17_Chr3g0079691 52.439 82 39 0 86 167 481 562 8.51e-27 105
Msa0658990 MtrunA17_Chr3g0079691 49.275 69 34 1 100 168 280 347 3.87e-13 66.6
Msa0658990 MtrunA17_Chr7g0243891 56.757 74 32 0 86 159 360 433 1.01e-26 105
Msa0658990 MtrunA17_Chr7g0243891 52.308 65 30 1 95 159 211 274 3.71e-15 72.4
Msa0658990 MtrunA17_Chr7g0249311 51.807 83 40 0 86 168 378 460 1.42e-26 105
Msa0658990 MtrunA17_Chr7g0249311 52.381 63 29 1 100 162 179 240 4.92e-13 66.2
Msa0658990 MtrunA17_Chr7g0249301 51.807 83 39 1 77 159 270 351 1.45e-26 103
Msa0658990 MtrunA17_Chr7g0249301 50.000 64 31 1 99 162 61 123 2.52e-12 63.9
Msa0658990 MtrunA17_Chr7g0224801 56.757 74 32 0 89 162 82 155 2.21e-26 98.6
Msa0658990 MtrunA17_Chr8g0335811 43.697 119 59 2 44 156 94 210 5.03e-26 99.4
Msa0658990 MtrunA17_Chr5g0418781 47.664 107 47 2 50 156 83 180 2.72e-25 98.6
Msa0658990 MtrunA17_Chr3g0106491 51.250 80 39 0 80 159 345 424 9.25e-25 99.8
Msa0658990 MtrunA17_Chr3g0106491 41.000 100 54 2 60 159 162 256 1.38e-14 70.9
Msa0658990 MtrunA17_Chr7g0248721 47.059 102 48 2 76 172 117 217 1.21e-24 95.5
Msa0658990 MtrunA17_Chr4g0068541 47.727 88 46 0 87 174 195 282 1.84e-24 98.2
Msa0658990 MtrunA17_Chr5g0432161 54.054 74 34 0 86 159 407 480 4.14e-24 98.2
Msa0658990 MtrunA17_Chr5g0432161 53.333 60 27 1 100 159 244 302 1.46e-13 67.8
Msa0658990 MtrunA17_Chr3g0129361 54.930 71 32 0 86 156 130 200 1.30e-23 92.8
Msa0658990 MtrunA17_Chr7g0263531 49.333 75 38 0 86 160 347 421 1.37e-22 93.2
Msa0658990 MtrunA17_Chr7g0263531 54.237 59 26 1 101 159 190 247 4.28e-12 63.5
Msa0658990 MtrunA17_Chr5g0447701 54.795 73 33 0 87 159 239 311 2.81e-22 92.0
Msa0658990 MtrunA17_Chr1g0189391 53.947 76 34 1 82 156 79 154 5.06e-22 90.5
Msa0658990 MtrunA17_Chr8g0386241 51.389 72 35 0 87 158 90 161 5.99e-22 87.4
Msa0658990 MtrunA17_Chr8g0383351 54.167 72 32 1 86 156 145 216 8.77e-22 88.2
Msa0658990 MtrunA17_Chr4g0074001 48.750 80 41 0 81 160 340 419 2.52e-21 90.1
Msa0658990 MtrunA17_Chr6g0485431 45.122 82 44 1 82 162 136 217 3.80e-21 88.6
Msa0658990 MtrunA17_Chr1g0188941 47.297 74 39 0 86 159 37 110 4.37e-21 83.2
Msa0658990 MtrunA17_Chr7g0270831 47.297 74 38 1 87 159 203 276 7.86e-21 88.2
Msa0658990 MtrunA17_Chr4g0075201 45.946 74 39 1 87 159 254 327 3.35e-20 86.7
Msa0658990 MtrunA17_Chr4g0051181 48.000 75 38 1 87 160 230 304 1.57e-19 84.7
Msa0658990 MtrunA17_Chr7g0238921 55.932 59 26 0 98 156 122 180 2.23e-19 83.2
Msa0658990 MtrunA17_Chr3g0099641 44.156 77 42 1 87 162 238 314 3.07e-19 84.0
Msa0658990 MtrunA17_Chr4g0053281 45.783 83 41 2 77 158 246 325 1.13e-18 81.6
Msa0658990 MtrunA17_Chr2g0322391 44.595 74 40 1 87 159 336 409 1.32e-18 82.4
Msa0658990 MtrunA17_Chr2g0302391 47.297 74 38 1 87 159 198 271 1.60e-18 81.6
Msa0658990 MtrunA17_Chr1g0147881 43.902 82 45 1 78 158 240 321 3.76e-18 80.1
Msa0658990 MtrunA17_Chr4g0060271 44.000 75 41 1 87 160 242 316 6.75e-18 80.1
Msa0658990 MtrunA17_Chr5g0401821 47.945 73 37 1 87 158 186 258 1.19e-17 77.8
Msa0658990 MtrunA17_Chr2g0288871 45.946 74 39 1 87 159 292 365 1.30e-17 79.3
Msa0658990 MtrunA17_Chr7g0216891 42.857 77 43 1 87 162 206 282 1.58e-17 79.3
Msa0658990 MtrunA17_Chr4g0025051 45.455 77 41 1 87 162 322 398 1.75e-17 79.0
Msa0658990 MtrunA17_Chr2g0333851 52.381 63 29 1 98 159 167 229 2.46e-17 77.8
Msa0658990 MtrunA17_Chr5g0447711 38.824 85 52 0 75 159 205 289 1.14e-16 75.9
Msa0658990 MtrunA17_Chr3g0136961 50.847 59 28 1 99 156 137 195 1.36e-16 75.5
Msa0658990 MtrunA17_Chr1g0184691 42.500 80 45 1 78 156 271 350 2.95e-16 75.1
Msa0658990 MtrunA17_Chr4g0001361 50.794 63 30 1 98 159 160 222 6.17e-16 73.9
Msa0658990 MtrunA17_Chr8g0379461 44.286 70 38 1 101 169 77 146 1.50e-15 72.0
Msa0658990 MtrunA17_Chr4g0025061 43.243 74 41 1 87 159 347 420 2.36e-15 72.8
Msa0658990 MtrunA17_Chr2g0293481 37.755 98 59 2 64 159 230 327 3.89e-15 72.4
Msa0658990 MtrunA17_Chr4g0071521 50.820 61 29 1 97 156 242 302 4.63e-15 71.2
Msa0658990 MtrunA17_Chr2g0319961 43.662 71 39 1 91 160 71 141 5.88e-15 70.5
Msa0658990 MtrunA17_Chr7g0270051 47.619 63 32 1 98 159 51 113 1.03e-14 70.1
Msa0658990 MtrunA17_Chr8g0348311 46.875 64 33 1 97 159 152 215 2.10e-14 69.7
Msa0658990 MtrunA17_Chr7g0228941 33.636 110 67 3 49 157 6 110 2.38e-14 66.2
Msa0658990 MtrunA17_Chr1g0150281 45.614 57 30 1 101 156 141 197 6.18e-14 68.2
Msa0658990 MtrunA17_Chr7g0248691 47.619 63 32 1 98 159 152 214 8.15e-14 68.2
Msa0658990 MtrunA17_Chr1g0202461 39.759 83 47 2 78 159 241 321 1.29e-13 67.8
Msa0658990 MtrunA17_Chr2g0289181 43.939 66 36 1 95 159 9 74 6.48e-13 64.7
Msa0658990 MtrunA17_Chr2g0284321 39.080 87 49 2 74 159 12 95 7.86e-13 63.9
Msa0658990 MtrunA17_Chr5g0432071 50.794 63 30 1 100 162 104 165 9.88e-13 63.5
Msa0658990 MtrunA17_Chr8g0387501 46.667 60 31 1 101 159 130 189 1.61e-12 63.9
Msa0658990 MtrunA17_Chr1g0150271 41.270 63 36 1 98 159 99 161 1.63e-12 63.9
Msa0658990 MtrunA17_Chr3g0139021 44.262 61 33 1 97 156 311 371 1.64e-12 64.7
Msa0658990 MtrunA17_Chr4g0024391 37.662 77 47 1 87 162 216 292 6.89e-12 62.8
Msa0658990 MtrunA17_Chr5g0402931 45.000 60 31 1 97 156 120 177 1.30e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0658990 AT5G64810.1 45.455 165 77 3 6 157 3 167 5.48e-39 132
Msa0658990 AT5G26170.1 75.714 70 17 0 87 156 100 169 8.64e-36 123
Msa0658990 AT5G49520.1 60.000 75 30 0 87 161 208 282 6.82e-29 110
Msa0658990 AT1G29860.1 61.333 75 29 0 87 161 123 197 2.09e-28 107
Msa0658990 AT4G18170.1 59.459 74 30 0 86 159 158 231 5.61e-28 106
Msa0658990 AT5G46350.1 49.451 91 46 0 86 176 169 259 1.46e-27 105
Msa0658990 AT2G47260.1 56.180 89 36 2 74 162 151 236 2.14e-27 105
Msa0658990 AT3G62340.1 61.333 75 29 0 86 160 104 178 2.48e-27 104
Msa0658990 AT3G01970.1 55.556 72 32 0 87 158 52 123 8.40e-27 99.4
Msa0658990 AT2G46130.1 55.128 78 35 0 79 156 9 86 1.20e-26 97.8
Msa0658990 AT2G38470.1 56.757 74 32 0 86 159 348 421 1.77e-26 105
Msa0658990 AT2G38470.1 51.667 60 28 1 100 159 184 242 1.60e-12 65.1
Msa0658990 AT5G41570.1 52.500 80 38 0 77 156 75 154 1.04e-25 97.4
Msa0658990 AT5G13080.1 56.944 72 31 0 87 158 54 125 1.08e-25 96.7
Msa0658990 AT1G69310.4 57.534 73 31 0 86 158 133 205 1.20e-25 100
Msa0658990 AT1G69310.2 57.534 73 31 0 86 158 133 205 1.20e-25 100
Msa0658990 AT1G69310.1 57.534 73 31 0 86 158 133 205 1.20e-25 100
Msa0658990 AT2G44745.1 44.444 126 66 3 35 159 81 203 1.49e-25 98.2
Msa0658990 AT5G56270.1 55.405 74 33 0 86 159 473 546 1.52e-25 102
Msa0658990 AT5G56270.1 53.333 60 27 1 100 159 273 331 5.74e-13 66.6
Msa0658990 AT3G01080.2 55.405 74 33 0 86 159 330 403 1.83e-25 101
Msa0658990 AT3G01080.2 53.333 60 27 1 100 159 205 263 2.81e-13 67.4
Msa0658990 AT3G01080.1 55.405 74 33 0 86 159 292 365 1.97e-25 101
Msa0658990 AT3G01080.1 53.333 60 27 1 100 159 167 225 3.50e-13 67.0
Msa0658990 AT1G13960.2 54.054 74 34 0 86 159 242 315 2.36e-25 100
Msa0658990 AT1G13960.2 50.667 75 33 2 88 159 61 134 6.57e-16 74.3
Msa0658990 AT5G07100.2 55.405 74 33 0 86 159 127 200 2.66e-25 97.4
Msa0658990 AT5G07100.2 41.772 79 44 1 100 176 24 102 3.51e-12 62.8
Msa0658990 AT5G07100.4 55.405 74 33 0 86 159 127 200 2.66e-25 97.4
Msa0658990 AT5G07100.4 41.772 79 44 1 100 176 24 102 3.51e-12 62.8
Msa0658990 AT5G07100.5 55.405 74 33 0 86 159 127 200 2.66e-25 97.4
Msa0658990 AT5G07100.5 41.772 79 44 1 100 176 24 102 3.51e-12 62.8
Msa0658990 AT5G07100.3 55.405 74 33 0 86 159 127 200 2.66e-25 97.4
Msa0658990 AT5G07100.3 41.772 79 44 1 100 176 24 102 3.51e-12 62.8
Msa0658990 AT2G21900.1 47.917 96 46 2 78 169 83 178 2.97e-25 97.1
Msa0658990 AT1G13960.1 54.054 74 34 0 86 159 395 468 3.45e-25 101
Msa0658990 AT1G13960.1 50.667 75 33 2 88 159 214 287 4.35e-16 75.5
Msa0658990 AT1G64000.1 53.659 82 38 0 75 156 89 170 3.66e-25 96.7
Msa0658990 AT4G26640.5 51.250 80 39 0 86 165 191 270 3.88e-25 100
Msa0658990 AT4G26640.5 50.000 62 30 1 98 159 33 93 2.17e-11 61.6
Msa0658990 AT4G26640.6 51.250 80 39 0 86 165 191 270 3.88e-25 100
Msa0658990 AT4G26640.6 50.000 62 30 1 98 159 33 93 2.17e-11 61.6
Msa0658990 AT4G26640.4 51.250 80 39 0 86 165 191 270 3.88e-25 100
Msa0658990 AT4G26640.4 50.000 62 30 1 98 159 33 93 2.17e-11 61.6
Msa0658990 AT4G26640.3 51.250 80 39 0 86 165 191 270 3.88e-25 100
Msa0658990 AT4G26640.3 50.000 62 30 1 98 159 33 93 2.17e-11 61.6
Msa0658990 AT5G07100.1 55.405 74 33 0 86 159 220 293 4.80e-25 98.6
Msa0658990 AT5G07100.1 41.772 79 44 1 100 176 117 195 2.97e-12 63.9
Msa0658990 AT4G26640.1 51.250 80 39 0 86 165 295 374 5.87e-25 100
Msa0658990 AT4G26640.1 50.000 62 30 1 98 159 137 197 1.24e-11 62.4
Msa0658990 AT4G26640.7 51.250 80 39 0 86 165 327 406 6.36e-25 100
Msa0658990 AT4G26640.7 50.000 62 30 1 98 159 169 229 1.22e-11 62.4
Msa0658990 AT4G26640.2 51.250 80 39 0 86 165 367 446 7.38e-25 100
Msa0658990 AT4G26640.2 50.000 62 30 1 98 159 209 269 1.22e-11 62.8
Msa0658990 AT2G03340.1 52.703 74 35 0 86 159 401 474 4.76e-24 98.2
Msa0658990 AT2G03340.1 56.667 60 25 1 100 159 250 308 3.77e-15 72.8
Msa0658990 AT4G39410.1 52.326 86 40 1 71 156 195 279 5.81e-24 95.9
Msa0658990 AT2G04880.2 42.857 112 59 2 51 159 233 342 5.98e-24 97.4
Msa0658990 AT2G04880.2 43.750 64 35 1 97 160 108 170 4.40e-11 60.8
Msa0658990 AT2G04880.1 42.857 112 59 2 51 159 257 366 6.11e-24 97.8
Msa0658990 AT2G04880.1 43.750 64 35 1 97 160 108 170 4.64e-11 60.8
Msa0658990 AT4G26440.1 48.780 82 42 0 86 167 358 439 1.02e-23 97.4
Msa0658990 AT4G26440.1 51.667 60 28 1 100 159 178 236 2.67e-11 61.6
Msa0658990 AT2G46130.2 52.564 78 33 1 79 156 9 82 3.71e-23 88.6
Msa0658990 AT2G30250.1 45.556 90 43 1 70 159 304 387 2.16e-22 92.8
Msa0658990 AT2G30250.1 48.649 74 36 2 100 172 166 238 3.89e-11 60.8
Msa0658990 AT1G69310.3 53.425 73 30 1 86 158 133 201 6.75e-22 90.1
Msa0658990 AT4G04450.2 47.297 74 38 1 87 159 185 258 1.59e-20 87.8
Msa0658990 AT5G43290.1 62.712 59 22 0 98 156 112 170 3.24e-20 85.1
Msa0658990 AT1G55600.1 43.529 85 48 0 75 159 282 366 4.09e-20 87.0
Msa0658990 AT4G04450.1 47.297 74 38 1 87 159 279 352 5.23e-20 86.7
Msa0658990 AT1G68150.1 46.667 75 39 1 87 160 222 296 1.40e-19 84.7
Msa0658990 AT2G23320.1 47.674 86 41 2 74 158 217 299 1.62e-19 84.0
Msa0658990 AT1G62300.1 47.297 74 38 1 87 159 299 372 2.71e-19 84.7
Msa0658990 AT4G30935.1 45.946 74 40 0 87 160 318 391 3.18e-19 84.3
Msa0658990 AT5G15130.2 44.156 77 42 1 87 162 172 248 3.92e-19 84.3
Msa0658990 AT5G15130.1 44.156 77 42 1 87 162 214 290 4.21e-19 84.0
Msa0658990 AT4G01720.1 44.595 74 40 1 87 159 226 299 1.73e-18 82.4
Msa0658990 AT1G18860.1 44.595 74 40 1 87 159 226 299 9.90e-18 80.1
Msa0658990 AT4G24240.1 53.968 63 28 1 97 158 278 340 1.66e-17 79.0
Msa0658990 AT3G04670.1 46.739 92 39 3 66 156 237 319 3.19e-17 77.8
Msa0658990 AT4G22070.4 47.297 74 38 1 87 159 284 357 3.62e-17 78.6
Msa0658990 AT4G22070.3 47.297 74 38 1 87 159 284 357 3.62e-17 78.6
Msa0658990 AT4G22070.2 47.297 74 38 1 87 159 284 357 3.62e-17 78.6
Msa0658990 AT4G22070.1 47.297 74 38 1 87 159 284 357 3.62e-17 78.6
Msa0658990 AT1G29280.1 50.794 63 30 1 101 162 75 137 3.84e-17 76.6
Msa0658990 AT2G25000.2 47.692 65 33 1 99 162 102 166 5.16e-17 75.9
Msa0658990 AT2G25000.4 47.692 65 33 1 99 162 102 166 5.16e-17 75.9
Msa0658990 AT2G25000.3 47.692 65 33 1 99 162 124 188 8.84e-17 75.5
Msa0658990 AT1G80840.1 46.032 63 33 1 98 159 144 206 1.10e-16 76.3
Msa0658990 AT5G28650.1 45.652 92 40 3 66 156 237 319 1.31e-16 76.3
Msa0658990 AT2G25000.1 47.692 65 33 1 99 162 145 209 1.44e-16 75.5
Msa0658990 AT2G24570.1 37.500 104 63 2 55 156 197 300 2.14e-16 75.5
Msa0658990 AT4G31550.2 47.727 88 36 4 72 156 222 302 2.85e-16 75.1
Msa0658990 AT2G34830.1 42.391 92 45 3 76 159 184 275 2.93e-16 75.9
Msa0658990 AT2G34830.2 42.391 92 45 3 76 159 193 284 2.99e-16 75.9
Msa0658990 AT4G31550.1 41.250 80 46 1 78 156 224 303 5.22e-16 74.3
Msa0658990 AT1G30650.1 40.860 93 47 3 76 160 186 278 1.16e-15 73.9
Msa0658990 AT3G58710.2 36.220 127 72 4 44 162 8 133 1.60e-15 72.4
Msa0658990 AT4G31800.2 43.939 66 36 1 99 163 110 175 1.84e-15 72.0
Msa0658990 AT4G31800.1 43.939 66 36 1 99 163 175 240 3.05e-15 72.4
Msa0658990 AT2G37260.3 46.875 64 34 0 95 158 264 327 5.25e-15 72.0
Msa0658990 AT2G37260.3 41.237 97 48 3 71 159 48 143 3.19e-13 67.0
Msa0658990 AT2G37260.2 46.875 64 34 0 95 158 262 325 5.36e-15 72.0
Msa0658990 AT2G37260.2 41.237 97 48 3 71 159 46 141 3.08e-13 67.0
Msa0658990 AT2G37260.1 46.875 64 34 0 95 158 344 407 5.43e-15 72.0
Msa0658990 AT2G37260.1 41.237 97 48 3 71 159 128 223 3.42e-13 67.0
Msa0658990 AT2G30590.1 52.459 61 28 1 97 156 310 370 6.09e-15 72.0
Msa0658990 AT3G58710.1 45.205 73 39 1 91 162 62 134 7.24e-15 70.9
Msa0658990 AT4G01250.1 50.000 60 29 1 101 159 129 188 6.21e-14 68.6
Msa0658990 AT4G23550.1 43.939 66 36 1 95 159 135 200 1.33e-13 67.8
Msa0658990 AT3G01080.3 53.333 60 27 1 100 159 205 263 1.45e-13 67.8
Msa0658990 AT1G69810.2 37.079 89 48 3 74 160 125 207 3.24e-13 66.6
Msa0658990 AT1G69810.1 37.079 89 48 3 74 160 183 265 4.89e-13 66.6
Msa0658990 AT5G52830.1 47.619 63 32 1 98 159 163 225 1.25e-12 65.1
Msa0658990 AT5G45050.4 48.333 60 30 1 101 159 1042 1101 2.44e-12 64.7
Msa0658990 AT5G45050.1 48.333 60 30 1 101 159 1181 1240 2.66e-12 64.7
Msa0658990 AT5G45050.2 48.333 60 30 1 101 159 1153 1212 2.84e-12 64.7
Msa0658990 AT1G66560.1 37.079 89 44 3 90 175 93 172 1.56e-11 61.2

Find 46 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAAACGGTTACTTTGTT+AGG 0.243599 4_3:-69227108 Msa0658990:three_prime_UTR
GAAGTAGAGTGAGCAAAATT+TGG 0.273680 4_3:+69229168 None:intergenic
AAGATCAGAGCTTGAGATTA+TGG 0.274000 4_3:-69228530 Msa0658990:CDS
ATGGGTTGTTGGTATATAAA+TGG 0.277706 4_3:+69229243 None:intergenic
CTTGTTCTAAAGGCTATCTT+TGG 0.312484 4_3:+69228550 None:intergenic
TGCATAGATAAACTCAGTTA+TGG 0.351269 4_3:+69229224 None:intergenic
TGCATCATTGAATTCTTGAT+TGG 0.365316 4_3:+69229032 None:intergenic
CAGAATGCACAAAAGAGATT+TGG 0.367718 4_3:+69227258 None:intergenic
AAATGGGATTAAGCGAAATA+AGG 0.381517 4_3:-69228584 Msa0658990:CDS
TTACATATTATTGTTCTTTG+AGG 0.400718 4_3:+69229008 None:intergenic
TTGCTTGATGATATTTGTAT+TGG 0.407278 4_3:-69229120 Msa0658990:CDS
GCATAGATAAACTCAGTTAT+GGG 0.408805 4_3:+69229225 None:intergenic
TGGCTGCAGTGGGAGGTAAA+TGG 0.416069 4_3:+69227278 None:intergenic
CAAAAGAGATTTGGCTGCAG+TGG 0.429261 4_3:+69227267 None:intergenic
TAAACTCAGTTATGGGTTGT+TGG 0.446707 4_3:+69229232 None:intergenic
TGTTACCTTCTCTAATGATT+CGG 0.455426 4_3:+69229065 None:intergenic
TGTGAAGAAAAGGGTGGAAA+GGG 0.465962 4_3:-69227360 Msa0658990:CDS
GGATACAAGTGGAGGAAGTA+CGG 0.474552 4_3:-69228502 Msa0658990:CDS
AAGCTCTGATCTTGTTCTAA+AGG 0.476991 4_3:+69228540 None:intergenic
GTGACTGAGATTCTTGATCA+AGG 0.485292 4_3:+69229094 None:intergenic
GAGCTTGAGATTATGGATGA+TGG 0.492012 4_3:-69228523 Msa0658990:CDS
TGGGATGTAATGTGAAGAAA+AGG 0.500798 4_3:-69227370 Msa0658990:CDS
GTGAGAGAATACAATTTGAG+AGG 0.523214 4_3:+69227186 None:intergenic
AAGCGAAATAAGGTAGAAGC+GGG 0.536791 4_3:-69228574 Msa0658990:CDS
ATGTGAAGAAAAGGGTGGAA+AGG 0.539725 4_3:-69227361 Msa0658990:CDS
AACTGAGTTTATCTATGCAA+TGG 0.539741 4_3:-69229220 Msa0658990:exon
CAAAATTTGGAGTAGCATGA+GGG 0.551934 4_3:+69229181 None:intergenic
GATACAAGTGGAGGAAGTAC+GGG 0.554483 4_3:-69228501 Msa0658990:CDS
TGAAGAACAGTCCTAACCTA+AGG 0.563814 4_3:-69228468 Msa0658990:intron
TATGTGATAACAAGTTATGA+AGG 0.564114 4_3:-69227320 Msa0658990:CDS
TACTACAAATGTTCAAGTGT+GGG 0.566302 4_3:-69227389 Msa0658990:CDS
TGAGAGAATACAATTTGAGA+GGG 0.572606 4_3:+69227187 None:intergenic
AAAAGAGATTTGGCTGCAGT+GGG 0.577682 4_3:+69227268 None:intergenic
ATATAAATGAGAAATGAAAA+CGG 0.580286 4_3:-69227121 Msa0658990:three_prime_UTR
GCAAAATTTGGAGTAGCATG+AGG 0.589246 4_3:+69229180 None:intergenic
CTACTACAAATGTTCAAGTG+TGG 0.597033 4_3:-69227390 Msa0658990:CDS
TTATGGATGATGGATACAAG+TGG 0.599156 4_3:-69228513 Msa0658990:CDS
AAGTACCGAATCATTAGAGA+AGG 0.606756 4_3:-69229070 Msa0658990:CDS
GGGAGGTAAATGGAATCTCA+TGG 0.608064 4_3:+69227288 None:intergenic
AGAGATTTGGCTGCAGTGGG+AGG 0.615268 4_3:+69227271 None:intergenic
TAAGCGAAATAAGGTAGAAG+CGG 0.616655 4_3:-69228575 Msa0658990:CDS
GCAGTGAGTTTGCACTGCAG+TGG 0.652818 4_3:+69227217 None:intergenic
TGGATGATGGATACAAGTGG+AGG 0.653822 4_3:-69228510 Msa0658990:CDS
GGGATGTAATGTGAAGAAAA+GGG 0.671707 4_3:-69227369 Msa0658990:CDS
ATGTAATGTGAAGAAAAGGG+TGG 0.682851 4_3:-69227366 Msa0658990:CDS
AGCGAAATAAGGTAGAAGCG+GGG 0.727454 4_3:-69228573 Msa0658990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCATTTAAAATTAAGAAAA+GGG - chr4_3:69227406-69227425 Msa0658990:intron 10.0%
!!! TTCATTTAAAATTAAGAAAA+GGG - chr4_3:69227406-69227425 Msa0658990:intron 10.0%
!!! GTTCATTTAAAATTAAGAAA+AGG - chr4_3:69227405-69227424 Msa0658990:intron 15.0%
!! CAAATACAAAATGAAATTTA+AGG + chr4_3:69227958-69227977 None:intergenic 15.0%
!!! TTGAAATTCAATTTTTAACT+CGG - chr4_3:69228226-69228245 Msa0658990:intron 15.0%
!! ATATAAATGAGAAATGAAAA+CGG - chr4_3:69229239-69229258 Msa0658990:five_prime_UTR 15.0%
!!! GTTCATTTAAAATTAAGAAA+AGG - chr4_3:69227405-69227424 Msa0658990:intron 15.0%
!! CAAATACAAAATGAAATTTA+AGG + chr4_3:69227958-69227977 None:intergenic 15.0%
!!! TTGAAATTCAATTTTTAACT+CGG - chr4_3:69228226-69228245 Msa0658990:intron 15.0%
!! ATATAAATGAGAAATGAAAA+CGG - chr4_3:69229239-69229258 Msa0658990:five_prime_UTR 15.0%
!! TTACATATTATTGTTCTTTG+AGG + chr4_3:69227355-69227374 None:intergenic 20.0%
!!! TAAAAAACATCATTTTGTTC+AGG + chr4_3:69227515-69227534 None:intergenic 20.0%
!!! CTAATAAAGTGTACATATTT+TGG - chr4_3:69227719-69227738 Msa0658990:intron 20.0%
!!! TTTTGCAGAAAATACAAAAA+TGG - chr4_3:69227759-69227778 Msa0658990:intron 20.0%
!! TTTGCAGAAAATACAAAAAT+GGG - chr4_3:69227760-69227779 Msa0658990:intron 20.0%
!! TAACTATAAGTTCATGATAT+TGG - chr4_3:69228080-69228099 Msa0658990:intron 20.0%
!! TATAAAATGTCAGATAACAA+GGG + chr4_3:69228498-69228517 None:intergenic 20.0%
!! ATATAAAATGTCAGATAACA+AGG + chr4_3:69228499-69228518 None:intergenic 20.0%
!! GTCTTAACATAAAATTAGTT+AGG - chr4_3:69228725-69228744 Msa0658990:intron 20.0%
!! TTTAGTGAAATTTCAAACTA+GGG + chr4_3:69228767-69228786 None:intergenic 20.0%
!! AAACATATAAAGTCAAAACA+AGG + chr4_3:69228853-69228872 None:intergenic 20.0%
!! AAAATGTAGTAACTAATCTT+CGG + chr4_3:69228901-69228920 None:intergenic 20.0%
!! TTACATATTATTGTTCTTTG+AGG + chr4_3:69227355-69227374 None:intergenic 20.0%
!!! TAAAAAACATCATTTTGTTC+AGG + chr4_3:69227515-69227534 None:intergenic 20.0%
!!! CTAATAAAGTGTACATATTT+TGG - chr4_3:69227719-69227738 Msa0658990:intron 20.0%
!!! TTTTGCAGAAAATACAAAAA+TGG - chr4_3:69227759-69227778 Msa0658990:intron 20.0%
!! TTTGCAGAAAATACAAAAAT+GGG - chr4_3:69227760-69227779 Msa0658990:intron 20.0%
!! TAACTATAAGTTCATGATAT+TGG - chr4_3:69228080-69228099 Msa0658990:intron 20.0%
!! TATAAAATGTCAGATAACAA+GGG + chr4_3:69228498-69228517 None:intergenic 20.0%
!! ATATAAAATGTCAGATAACA+AGG + chr4_3:69228499-69228518 None:intergenic 20.0%
!! GTCTTAACATAAAATTAGTT+AGG - chr4_3:69228725-69228744 Msa0658990:intron 20.0%
!! TTTAGTGAAATTTCAAACTA+GGG + chr4_3:69228767-69228786 None:intergenic 20.0%
!! AAACATATAAAGTCAAAACA+AGG + chr4_3:69228853-69228872 None:intergenic 20.0%
!! AAAATGTAGTAACTAATCTT+CGG + chr4_3:69228901-69228920 None:intergenic 20.0%
!! TTGCTTGATGATATTTGTAT+TGG - chr4_3:69227240-69227259 Msa0658990:exon 25.0%
! ACAAGAGCAATACAAATTAA+GGG + chr4_3:69227387-69227406 None:intergenic 25.0%
! AACAAGAGCAATACAAATTA+AGG + chr4_3:69227388-69227407 None:intergenic 25.0%
!! TCAAAAGTGTACTTCTAAAT+GGG + chr4_3:69227468-69227487 None:intergenic 25.0%
!! TTCAAAAGTGTACTTCTAAA+TGG + chr4_3:69227469-69227488 None:intergenic 25.0%
!!! TCTAAAAGTGTACATTTTGA+GGG - chr4_3:69227538-69227557 Msa0658990:intron 25.0%
! ATTAATGAAGCATATACCAT+CGG - chr4_3:69227650-69227669 Msa0658990:intron 25.0%
!! GTATTTCCTTTTATTACCTT+AGG + chr4_3:69227911-69227930 None:intergenic 25.0%
!! ATTTGAACAAAGTAGCTTTA+AGG - chr4_3:69227973-69227992 Msa0658990:intron 25.0%
! AAACCGAAGAAAATGAATAT+TGG - chr4_3:69228133-69228152 Msa0658990:intron 25.0%
!! CTACCAATATTCATTTTCTT+CGG + chr4_3:69228139-69228158 None:intergenic 25.0%
! AATTAGTTAGGTTTGACAAA+TGG - chr4_3:69228737-69228756 Msa0658990:intron 25.0%
! CTTTAGTGAAATTTCAAACT+AGG + chr4_3:69228768-69228787 None:intergenic 25.0%
! AGATGATAAAAAACGAGATT+TGG - chr4_3:69228794-69228813 Msa0658990:intron 25.0%
! TATTGAATTAATGATGACAC+AGG - chr4_3:69228945-69228964 Msa0658990:intron 25.0%
! TATGTGATAACAAGTTATGA+AGG - chr4_3:69229040-69229059 Msa0658990:CDS 25.0%
!! TTGCTTGATGATATTTGTAT+TGG - chr4_3:69227240-69227259 Msa0658990:exon 25.0%
! ACAAGAGCAATACAAATTAA+GGG + chr4_3:69227387-69227406 None:intergenic 25.0%
! AACAAGAGCAATACAAATTA+AGG + chr4_3:69227388-69227407 None:intergenic 25.0%
!! TCAAAAGTGTACTTCTAAAT+GGG + chr4_3:69227468-69227487 None:intergenic 25.0%
!! TTCAAAAGTGTACTTCTAAA+TGG + chr4_3:69227469-69227488 None:intergenic 25.0%
!!! TCTAAAAGTGTACATTTTGA+GGG - chr4_3:69227538-69227557 Msa0658990:intron 25.0%
! ATTAATGAAGCATATACCAT+CGG - chr4_3:69227650-69227669 Msa0658990:intron 25.0%
!! GTATTTCCTTTTATTACCTT+AGG + chr4_3:69227911-69227930 None:intergenic 25.0%
!! ATTTGAACAAAGTAGCTTTA+AGG - chr4_3:69227973-69227992 Msa0658990:intron 25.0%
! AAACCGAAGAAAATGAATAT+TGG - chr4_3:69228133-69228152 Msa0658990:intron 25.0%
!! CTACCAATATTCATTTTCTT+CGG + chr4_3:69228139-69228158 None:intergenic 25.0%
! AATTAGTTAGGTTTGACAAA+TGG - chr4_3:69228737-69228756 Msa0658990:intron 25.0%
! CTTTAGTGAAATTTCAAACT+AGG + chr4_3:69228768-69228787 None:intergenic 25.0%
! AGATGATAAAAAACGAGATT+TGG - chr4_3:69228794-69228813 Msa0658990:intron 25.0%
! TATTGAATTAATGATGACAC+AGG - chr4_3:69228945-69228964 Msa0658990:intron 25.0%
! TATGTGATAACAAGTTATGA+AGG - chr4_3:69229040-69229059 Msa0658990:CDS 25.0%
! ATGGGTTGTTGGTATATAAA+TGG + chr4_3:69227120-69227139 None:intergenic 30.0%
GCATAGATAAACTCAGTTAT+GGG + chr4_3:69227138-69227157 None:intergenic 30.0%
TGCATAGATAAACTCAGTTA+TGG + chr4_3:69227139-69227158 None:intergenic 30.0%
AACTGAGTTTATCTATGCAA+TGG - chr4_3:69227140-69227159 Msa0658990:three_prime_UTR 30.0%
TGTTACCTTCTCTAATGATT+CGG + chr4_3:69227298-69227317 None:intergenic 30.0%
TGCATCATTGAATTCTTGAT+TGG + chr4_3:69227331-69227350 None:intergenic 30.0%
!! ATCGGTTTTTAAGAAACTAC+GGG + chr4_3:69227445-69227464 None:intergenic 30.0%
!! GATCGGTTTTTAAGAAACTA+CGG + chr4_3:69227446-69227465 None:intergenic 30.0%
!! GTCTAAAAGTGTACATTTTG+AGG - chr4_3:69227537-69227556 Msa0658990:intron 30.0%
!! AGTGTACATATTTTGGATTG+TGG - chr4_3:69227726-69227745 Msa0658990:intron 30.0%
!! GTGTACATATTTTGGATTGT+GGG - chr4_3:69227727-69227746 Msa0658990:intron 30.0%
!! TGTACATATTTTGGATTGTG+GGG - chr4_3:69227728-69227747 Msa0658990:intron 30.0%
AAATGGGATTAAGCGAAATA+AGG - chr4_3:69227776-69227795 Msa0658990:intron 30.0%
TCCTAACCTAAGGTAATAAA+AGG - chr4_3:69227902-69227921 Msa0658990:intron 30.0%
! TCCTTTTATTACCTTAGGTT+AGG + chr4_3:69227906-69227925 None:intergenic 30.0%
TTCAAACATCATAGTACGAT+AGG + chr4_3:69228019-69228038 None:intergenic 30.0%
! ATGATATTGGTCTACTCATT+TGG - chr4_3:69228093-69228112 Msa0658990:intron 30.0%
! TGATATTGGTCTACTCATTT+GGG - chr4_3:69228094-69228113 Msa0658990:intron 30.0%
AATACAATTGATGAGATGTG+TGG + chr4_3:69228418-69228437 None:intergenic 30.0%
AGTTAAACTTAACTAACCAG+TGG - chr4_3:69228608-69228627 Msa0658990:intron 30.0%
TACTACAAATGTTCAAGTGT+GGG - chr4_3:69228971-69228990 Msa0658990:intron 30.0%
TGAGAGAATACAATTTGAGA+GGG + chr4_3:69229176-69229195 None:intergenic 30.0%
! ATGAAAACGGTTACTTTGTT+AGG - chr4_3:69229252-69229271 Msa0658990:five_prime_UTR 30.0%
! ATGGGTTGTTGGTATATAAA+TGG + chr4_3:69227120-69227139 None:intergenic 30.0%
GCATAGATAAACTCAGTTAT+GGG + chr4_3:69227138-69227157 None:intergenic 30.0%
TGCATAGATAAACTCAGTTA+TGG + chr4_3:69227139-69227158 None:intergenic 30.0%
AACTGAGTTTATCTATGCAA+TGG - chr4_3:69227140-69227159 Msa0658990:three_prime_UTR 30.0%
TGTTACCTTCTCTAATGATT+CGG + chr4_3:69227298-69227317 None:intergenic 30.0%
TGCATCATTGAATTCTTGAT+TGG + chr4_3:69227331-69227350 None:intergenic 30.0%
!! ATCGGTTTTTAAGAAACTAC+GGG + chr4_3:69227445-69227464 None:intergenic 30.0%
!! GATCGGTTTTTAAGAAACTA+CGG + chr4_3:69227446-69227465 None:intergenic 30.0%
!! GTCTAAAAGTGTACATTTTG+AGG - chr4_3:69227537-69227556 Msa0658990:intron 30.0%
!! AGTGTACATATTTTGGATTG+TGG - chr4_3:69227726-69227745 Msa0658990:intron 30.0%
!! GTGTACATATTTTGGATTGT+GGG - chr4_3:69227727-69227746 Msa0658990:intron 30.0%
!! TGTACATATTTTGGATTGTG+GGG - chr4_3:69227728-69227747 Msa0658990:intron 30.0%
AAATGGGATTAAGCGAAATA+AGG - chr4_3:69227776-69227795 Msa0658990:intron 30.0%
TCCTAACCTAAGGTAATAAA+AGG - chr4_3:69227902-69227921 Msa0658990:intron 30.0%
! TCCTTTTATTACCTTAGGTT+AGG + chr4_3:69227906-69227925 None:intergenic 30.0%
TTCAAACATCATAGTACGAT+AGG + chr4_3:69228019-69228038 None:intergenic 30.0%
! ATGATATTGGTCTACTCATT+TGG - chr4_3:69228093-69228112 Msa0658990:intron 30.0%
! TGATATTGGTCTACTCATTT+GGG - chr4_3:69228094-69228113 Msa0658990:intron 30.0%
AATACAATTGATGAGATGTG+TGG + chr4_3:69228418-69228437 None:intergenic 30.0%
AGTTAAACTTAACTAACCAG+TGG - chr4_3:69228608-69228627 Msa0658990:intron 30.0%
TACTACAAATGTTCAAGTGT+GGG - chr4_3:69228971-69228990 Msa0658990:intron 30.0%
TGAGAGAATACAATTTGAGA+GGG + chr4_3:69229176-69229195 None:intergenic 30.0%
! ATGAAAACGGTTACTTTGTT+AGG - chr4_3:69229252-69229271 Msa0658990:five_prime_UTR 30.0%
! TAAACTCAGTTATGGGTTGT+TGG + chr4_3:69227131-69227150 None:intergenic 35.0%
! CAAAATTTGGAGTAGCATGA+GGG + chr4_3:69227182-69227201 None:intergenic 35.0%
GAAGTAGAGTGAGCAAAATT+TGG + chr4_3:69227195-69227214 None:intergenic 35.0%
!! AAGTACCGAATCATTAGAGA+AGG - chr4_3:69227290-69227309 Msa0658990:CDS 35.0%
GCAATACAAATTAAGGGAGA+AGG + chr4_3:69227381-69227400 None:intergenic 35.0%
!! TCGGTTTTTAAGAAACTACG+GGG + chr4_3:69227444-69227463 None:intergenic 35.0%
AGTGTACTTCTAAATGGGAT+CGG + chr4_3:69227463-69227482 None:intergenic 35.0%
!! AAGTGTACATTTTGAGGGAT+CGG - chr4_3:69227543-69227562 Msa0658990:intron 35.0%
!! GGATCGGTTTTTAGAAATCA+TGG - chr4_3:69227559-69227578 Msa0658990:intron 35.0%
!!! TGCATGATTGCGTTTTTTCA+AGG + chr4_3:69227599-69227618 None:intergenic 35.0%
CAAATGAAGTAATGAACCGA+TGG + chr4_3:69227669-69227688 None:intergenic 35.0%
TAAGCGAAATAAGGTAGAAG+CGG - chr4_3:69227785-69227804 Msa0658990:intron 35.0%
!! CTTGTTCTAAAGGCTATCTT+TGG + chr4_3:69227813-69227832 None:intergenic 35.0%
! AAGCTCTGATCTTGTTCTAA+AGG + chr4_3:69227823-69227842 None:intergenic 35.0%
AAGATCAGAGCTTGAGATTA+TGG - chr4_3:69227830-69227849 Msa0658990:intron 35.0%
!! TTATGGATGATGGATACAAG+TGG - chr4_3:69227847-69227866 Msa0658990:intron 35.0%
!! TGAACAAAGTAGCTTTAAGG+TGG - chr4_3:69227976-69227995 Msa0658990:intron 35.0%
! GATATTGGTCTACTCATTTG+GGG - chr4_3:69228095-69228114 Msa0658990:intron 35.0%
TTTAAGCATCAATCCATCGT+AGG - chr4_3:69228380-69228399 Msa0658990:intron 35.0%
TTAAGCATCAATCCATCGTA+GGG - chr4_3:69228381-69228400 Msa0658990:intron 35.0%
ACTTAACTAACCAGTGGATA+AGG - chr4_3:69228614-69228633 Msa0658990:intron 35.0%
TAGGTAGCTAGATAATGTTG+TGG - chr4_3:69228655-69228674 Msa0658990:intron 35.0%
AGTGAAATTTCAAACTAGGG+TGG + chr4_3:69228764-69228783 None:intergenic 35.0%
CTACTACAAATGTTCAAGTG+TGG - chr4_3:69228970-69228989 Msa0658990:intron 35.0%
TGGGATGTAATGTGAAGAAA+AGG - chr4_3:69228990-69229009 Msa0658990:intron 35.0%
GGGATGTAATGTGAAGAAAA+GGG - chr4_3:69228991-69229010 Msa0658990:intron 35.0%
ATGTAATGTGAAGAAAAGGG+TGG - chr4_3:69228994-69229013 Msa0658990:intron 35.0%
CAGAATGCACAAAAGAGATT+TGG + chr4_3:69229105-69229124 None:intergenic 35.0%
GTGAGAGAATACAATTTGAG+AGG + chr4_3:69229177-69229196 None:intergenic 35.0%
! TAAACTCAGTTATGGGTTGT+TGG + chr4_3:69227131-69227150 None:intergenic 35.0%
! CAAAATTTGGAGTAGCATGA+GGG + chr4_3:69227182-69227201 None:intergenic 35.0%
GAAGTAGAGTGAGCAAAATT+TGG + chr4_3:69227195-69227214 None:intergenic 35.0%
!! AAGTACCGAATCATTAGAGA+AGG - chr4_3:69227290-69227309 Msa0658990:CDS 35.0%
GCAATACAAATTAAGGGAGA+AGG + chr4_3:69227381-69227400 None:intergenic 35.0%
!! TCGGTTTTTAAGAAACTACG+GGG + chr4_3:69227444-69227463 None:intergenic 35.0%
AGTGTACTTCTAAATGGGAT+CGG + chr4_3:69227463-69227482 None:intergenic 35.0%
!! AAGTGTACATTTTGAGGGAT+CGG - chr4_3:69227543-69227562 Msa0658990:intron 35.0%
!! GGATCGGTTTTTAGAAATCA+TGG - chr4_3:69227559-69227578 Msa0658990:intron 35.0%
!!! TGCATGATTGCGTTTTTTCA+AGG + chr4_3:69227599-69227618 None:intergenic 35.0%
CAAATGAAGTAATGAACCGA+TGG + chr4_3:69227669-69227688 None:intergenic 35.0%
TAAGCGAAATAAGGTAGAAG+CGG - chr4_3:69227785-69227804 Msa0658990:intron 35.0%
!! CTTGTTCTAAAGGCTATCTT+TGG + chr4_3:69227813-69227832 None:intergenic 35.0%
! AAGCTCTGATCTTGTTCTAA+AGG + chr4_3:69227823-69227842 None:intergenic 35.0%
AAGATCAGAGCTTGAGATTA+TGG - chr4_3:69227830-69227849 Msa0658990:intron 35.0%
!! TTATGGATGATGGATACAAG+TGG - chr4_3:69227847-69227866 Msa0658990:intron 35.0%
!! TGAACAAAGTAGCTTTAAGG+TGG - chr4_3:69227976-69227995 Msa0658990:intron 35.0%
! GATATTGGTCTACTCATTTG+GGG - chr4_3:69228095-69228114 Msa0658990:intron 35.0%
TTTAAGCATCAATCCATCGT+AGG - chr4_3:69228380-69228399 Msa0658990:intron 35.0%
TTAAGCATCAATCCATCGTA+GGG - chr4_3:69228381-69228400 Msa0658990:intron 35.0%
ACTTAACTAACCAGTGGATA+AGG - chr4_3:69228614-69228633 Msa0658990:intron 35.0%
TAGGTAGCTAGATAATGTTG+TGG - chr4_3:69228655-69228674 Msa0658990:intron 35.0%
AGTGAAATTTCAAACTAGGG+TGG + chr4_3:69228764-69228783 None:intergenic 35.0%
CTACTACAAATGTTCAAGTG+TGG - chr4_3:69228970-69228989 Msa0658990:intron 35.0%
TGGGATGTAATGTGAAGAAA+AGG - chr4_3:69228990-69229009 Msa0658990:intron 35.0%
GGGATGTAATGTGAAGAAAA+GGG - chr4_3:69228991-69229010 Msa0658990:intron 35.0%
ATGTAATGTGAAGAAAAGGG+TGG - chr4_3:69228994-69229013 Msa0658990:intron 35.0%
CAGAATGCACAAAAGAGATT+TGG + chr4_3:69229105-69229124 None:intergenic 35.0%
GTGAGAGAATACAATTTGAG+AGG + chr4_3:69229177-69229196 None:intergenic 35.0%
! GCAAAATTTGGAGTAGCATG+AGG + chr4_3:69227183-69227202 None:intergenic 40.0%
GTGACTGAGATTCTTGATCA+AGG + chr4_3:69227269-69227288 None:intergenic 40.0%
AAGCGAAATAAGGTAGAAGC+GGG - chr4_3:69227786-69227805 Msa0658990:intron 40.0%
GAGCTTGAGATTATGGATGA+TGG - chr4_3:69227837-69227856 Msa0658990:intron 40.0%
TGAAGAACAGTCCTAACCTA+AGG - chr4_3:69227892-69227911 Msa0658990:intron 40.0%
! CTACTCATTTGGGGTTGAAT+CGG - chr4_3:69228104-69228123 Msa0658990:intron 40.0%
ACTAACCAGTGGATAAGGAT+AGG - chr4_3:69228619-69228638 Msa0658990:intron 40.0%
CTAACCAGTGGATAAGGATA+GGG - chr4_3:69228620-69228639 Msa0658990:intron 40.0%
GTAGCTAGATAATGTTGTGG+CGG - chr4_3:69228658-69228677 Msa0658990:intron 40.0%
CTGACTAATGGCTTGAGATA+GGG - chr4_3:69228693-69228712 Msa0658990:intron 40.0%
ATGTGAAGAAAAGGGTGGAA+AGG - chr4_3:69228999-69229018 Msa0658990:intron 40.0%
TGTGAAGAAAAGGGTGGAAA+GGG - chr4_3:69229000-69229019 Msa0658990:intron 40.0%
AAAAGAGATTTGGCTGCAGT+GGG + chr4_3:69229095-69229114 None:intergenic 40.0%
! GCAAAATTTGGAGTAGCATG+AGG + chr4_3:69227183-69227202 None:intergenic 40.0%
GTGACTGAGATTCTTGATCA+AGG + chr4_3:69227269-69227288 None:intergenic 40.0%
AAGCGAAATAAGGTAGAAGC+GGG - chr4_3:69227786-69227805 Msa0658990:intron 40.0%
GAGCTTGAGATTATGGATGA+TGG - chr4_3:69227837-69227856 Msa0658990:intron 40.0%
TGAAGAACAGTCCTAACCTA+AGG - chr4_3:69227892-69227911 Msa0658990:intron 40.0%
! CTACTCATTTGGGGTTGAAT+CGG - chr4_3:69228104-69228123 Msa0658990:intron 40.0%
ACTAACCAGTGGATAAGGAT+AGG - chr4_3:69228619-69228638 Msa0658990:intron 40.0%
CTAACCAGTGGATAAGGATA+GGG - chr4_3:69228620-69228639 Msa0658990:intron 40.0%
GTAGCTAGATAATGTTGTGG+CGG - chr4_3:69228658-69228677 Msa0658990:intron 40.0%
CTGACTAATGGCTTGAGATA+GGG - chr4_3:69228693-69228712 Msa0658990:intron 40.0%
ATGTGAAGAAAAGGGTGGAA+AGG - chr4_3:69228999-69229018 Msa0658990:intron 40.0%
TGTGAAGAAAAGGGTGGAAA+GGG - chr4_3:69229000-69229019 Msa0658990:intron 40.0%
AAAAGAGATTTGGCTGCAGT+GGG + chr4_3:69229095-69229114 None:intergenic 40.0%
AGCGAAATAAGGTAGAAGCG+GGG - chr4_3:69227787-69227806 Msa0658990:intron 45.0%
!! TGGATGATGGATACAAGTGG+AGG - chr4_3:69227850-69227869 Msa0658990:intron 45.0%
GGATACAAGTGGAGGAAGTA+CGG - chr4_3:69227858-69227877 Msa0658990:intron 45.0%
! GATACAAGTGGAGGAAGTAC+GGG - chr4_3:69227859-69227878 Msa0658990:intron 45.0%
TTGATGAGATGTGTGGTGTC+AGG + chr4_3:69228411-69228430 None:intergenic 45.0%
TAAGGATAGGGCAGTACTTC+AGG - chr4_3:69228632-69228651 Msa0658990:intron 45.0%
!! CTGGTCATAGTGCTGACTAA+TGG - chr4_3:69228681-69228700 Msa0658990:intron 45.0%
GCTGACTAATGGCTTGAGAT+AGG - chr4_3:69228692-69228711 Msa0658990:intron 45.0%
GGGAGGTAAATGGAATCTCA+TGG + chr4_3:69229075-69229094 None:intergenic 45.0%
CAAAAGAGATTTGGCTGCAG+TGG + chr4_3:69229096-69229115 None:intergenic 45.0%
AGCGAAATAAGGTAGAAGCG+GGG - chr4_3:69227787-69227806 Msa0658990:intron 45.0%
!! TGGATGATGGATACAAGTGG+AGG - chr4_3:69227850-69227869 Msa0658990:intron 45.0%
GGATACAAGTGGAGGAAGTA+CGG - chr4_3:69227858-69227877 Msa0658990:intron 45.0%
! GATACAAGTGGAGGAAGTAC+GGG - chr4_3:69227859-69227878 Msa0658990:intron 45.0%
TTGATGAGATGTGTGGTGTC+AGG + chr4_3:69228411-69228430 None:intergenic 45.0%
TAAGGATAGGGCAGTACTTC+AGG - chr4_3:69228632-69228651 Msa0658990:intron 45.0%
!! CTGGTCATAGTGCTGACTAA+TGG - chr4_3:69228681-69228700 Msa0658990:intron 45.0%
GCTGACTAATGGCTTGAGAT+AGG - chr4_3:69228692-69228711 Msa0658990:intron 45.0%
GGGAGGTAAATGGAATCTCA+TGG + chr4_3:69229075-69229094 None:intergenic 45.0%
CAAAAGAGATTTGGCTGCAG+TGG + chr4_3:69229096-69229115 None:intergenic 45.0%
!!! ACATTTTATATTTTTTTTAA+AGG - chr4_3:69228508-69228527 Msa0658990:CDS 5.0%
!!! ACATTTTATATTTTTTTTAA+AGG - chr4_3:69228508-69228527 Msa0658990:CDS 5.0%
ACTGCCCTATCCTTATCCAC+TGG + chr4_3:69228627-69228646 None:intergenic 50.0%
GATAGGGCAGTACTTCAGGT+AGG - chr4_3:69228636-69228655 Msa0658990:intron 50.0%
CTAGATAATGTTGTGGCGGC+TGG - chr4_3:69228662-69228681 Msa0658990:intron 50.0%
ACTGCCCTATCCTTATCCAC+TGG + chr4_3:69228627-69228646 None:intergenic 50.0%
GATAGGGCAGTACTTCAGGT+AGG - chr4_3:69228636-69228655 Msa0658990:intron 50.0%
CTAGATAATGTTGTGGCGGC+TGG - chr4_3:69228662-69228681 Msa0658990:intron 50.0%
GTGTCAGGTACTCCCTACGA+TGG + chr4_3:69228396-69228415 None:intergenic 55.0%
TGGCTGCAGTGGGAGGTAAA+TGG + chr4_3:69229085-69229104 None:intergenic 55.0%
AGAGATTTGGCTGCAGTGGG+AGG + chr4_3:69229092-69229111 None:intergenic 55.0%
GCAGTGAGTTTGCACTGCAG+TGG + chr4_3:69229146-69229165 None:intergenic 55.0%
GTGTCAGGTACTCCCTACGA+TGG + chr4_3:69228396-69228415 None:intergenic 55.0%
TGGCTGCAGTGGGAGGTAAA+TGG + chr4_3:69229085-69229104 None:intergenic 55.0%
AGAGATTTGGCTGCAGTGGG+AGG + chr4_3:69229092-69229111 None:intergenic 55.0%
GCAGTGAGTTTGCACTGCAG+TGG + chr4_3:69229146-69229165 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 69227090 69229292 69227090 ID=Msa0658990;Name=Msa0658990
chr4_3 mRNA 69227090 69229292 69227090 ID=Msa0658990-mRNA-1;Parent=Msa0658990;Name=Msa0658990-mRNA-1;_AED=0.14;_eAED=0.14;_QI=69|1|1|1|1|1|3|154|176
chr4_3 exon 69227090 69227415 69227090 ID=Msa0658990-mRNA-1:exon:20291;Parent=Msa0658990-mRNA-1
chr4_3 exon 69228469 69228615 69228469 ID=Msa0658990-mRNA-1:exon:20290;Parent=Msa0658990-mRNA-1
chr4_3 exon 69229012 69229292 69229012 ID=Msa0658990-mRNA-1:exon:20289;Parent=Msa0658990-mRNA-1
chr4_3 five_prime_UTR 69229224 69229292 69229224 ID=Msa0658990-mRNA-1:five_prime_utr;Parent=Msa0658990-mRNA-1
chr4_3 CDS 69229012 69229223 69229012 ID=Msa0658990-mRNA-1:cds;Parent=Msa0658990-mRNA-1
chr4_3 CDS 69228469 69228615 69228469 ID=Msa0658990-mRNA-1:cds;Parent=Msa0658990-mRNA-1
chr4_3 CDS 69227244 69227415 69227244 ID=Msa0658990-mRNA-1:cds;Parent=Msa0658990-mRNA-1
chr4_3 three_prime_UTR 69227090 69227243 69227090 ID=Msa0658990-mRNA-1:three_prime_utr;Parent=Msa0658990-mRNA-1
Gene Sequence

>Msa0658990

ATGGACTACTATTTTGTAAACCCTCATGCTACTCCAAATTTTGCTCACTCTACTTCTGAGTTTATACTATCTGATTATCTTTTGCTTGATGATATTTGTATTGGTCACCTTGATCAAGAATCTCAGTCACAAAGTACCGAATCATTAGAGAAGGTAACATTCAATGATGCCAATCAAGAATTCAATGATGCAACCTCAAAGAACAATAATATAAAATACAAAAATGGGATTAAGCGAAATAAGGTAGAAGCGGGGCCAAAGATAGCCTTTAGAACAAGATCAGAGCTTGAGATTATGGATGATGGATACAAGTGGAGGAAGTACGGGAAGAAGTCTGTGAAGAACAGTCCTAACCTAAGGAACTACTACAAATGTTCAAGTGTGGGATGTAATGTGAAGAAAAGGGTGGAAAGGGATAGAGATGATTCGAGCTATGTGATAACAAGTTATGAAGGTGTGCATAACCATGAGATTCCATTTACCTCCCACTGCAGCCAAATCTCTTTTGTGCATTCTGATTCATATAATTAA
Protein sequence

>Msa0658990

MDYYFVNPHATPNFAHSTSEFILSDYLLLDDICIGHLDQESQSQSTESLEKVTFNDANQEFNDATSKNNNIKYKNGIKRNKVEAGPKIAFRTRSELEIMDDGYKWRKYGKKSVKNSPNLRNYYKCSSVGCNVKKRVERDRDDSSYVITSYEGVHNHEIPFTSHCSQISFVHSDSYN*