AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0942190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0942190 MtrunA17_Chr5g0394201 99.432 176 1 0 2 177 175 350 1.00e-115 352
Msa0942190 MtrunA17_Chr1g0205891 80.435 184 28 2 2 177 180 363 2.29e-86 274
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0942190 AT3G06010.1 69.942 173 45 2 12 177 218 390 6.80e-71 232
Msa0942190 AT5G19310.1 65.476 168 56 2 12 177 206 373 1.36e-56 191
Msa0942190 AT5G19310.2 59.259 108 42 2 72 177 2 109 3.62e-24 99.0

Find 31 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATAAACTGCTTGTAAATT+TGG 0.073163 6_3:-16570800 Msa0942190:CDS
TTCAATACCATTTGATTGTT+TGG 0.257333 6_3:+16570750 None:intergenic
TTTGAAGAATGGAATTTCTA+AGG 0.289082 6_3:-16570707 Msa0942190:CDS
AAGAACCTTGGATTTGTAAC+TGG 0.305795 6_3:+16571335 None:intergenic
AATAAACTGCTTGTAAATTT+GGG 0.333637 6_3:-16570799 Msa0942190:CDS
TGAGGAAGATGAGGATTTGA+TGG 0.338325 6_3:-16570674 Msa0942190:CDS
ACAGAGGAATGATGCTGTTC+AGG 0.361881 6_3:-16571299 Msa0942190:intron
GATTCAGATGCTTTGAAGAA+TGG 0.397442 6_3:-16570718 Msa0942190:CDS
ATCAAATGGTATTGAACCCT+TGG 0.429598 6_3:-16570743 Msa0942190:CDS
GACAGCAGTGATTTACTTGA+AGG 0.434120 6_3:-16570634 Msa0942190:CDS
CATACATTCCATTCAAGAAA+AGG 0.438102 6_3:-16570590 None:intergenic
AGCTTTAAAAGCTGATGATC+AGG 0.449727 6_3:-16570887 Msa0942190:CDS
TTCTCCTCTTCAAAGAACCT+TGG 0.472374 6_3:+16571323 None:intergenic
AGAATAGAAGAGCAAGCAAA+AGG 0.502984 6_3:-16571418 Msa0942190:CDS
CAAAGCATCTGAATCTTCCA+AGG 0.504030 6_3:+16570726 None:intergenic
AGAGACTCCAAACAATCAAA+TGG 0.519227 6_3:-16570757 Msa0942190:CDS
GCAAAGACAACGTGCAACTC+GGG 0.524817 6_3:-16570929 Msa0942190:CDS
TATTGCAGGCATGGCATGGA+AGG 0.525468 6_3:-16570951 Msa0942190:intron
ACAATATTGCAGGCATGGCA+TGG 0.526024 6_3:-16570955 Msa0942190:intron
GGCAAAGACAACGTGCAACT+CGG 0.526390 6_3:-16570930 Msa0942190:CDS
AAATCCAAGGTTCTTTGAAG+AGG 0.529452 6_3:-16571327 Msa0942190:CDS
TTCTAAGGAATCACCTCTTG+AGG 0.538019 6_3:-16570692 Msa0942190:CDS
AAATCCTCATCTTCCTCAAG+AGG 0.550669 6_3:+16570679 None:intergenic
TCAGGAAGCTTACATGAGAA+TGG 0.557139 6_3:-16570869 Msa0942190:CDS
GAGTTCCAGTTACAAATCCA+AGG 0.578754 6_3:-16571340 Msa0942190:CDS
AAAGCATCTGAATCTTCCAA+GGG 0.588440 6_3:+16570727 None:intergenic
GGATTTGTAACTGGAACTCA+CGG 0.597702 6_3:+16571344 None:intergenic
ATCACCTCTTGAGGAAGATG+AGG 0.604453 6_3:-16570683 Msa0942190:CDS
GTGATTTACTTGAAGGTCAG+CGG 0.616779 6_3:-16570627 Msa0942190:CDS
GCTTGTAAATTTGGGAGCAG+CGG 0.624800 6_3:-16570791 Msa0942190:CDS
CTTTGAAGAGGAGAAAACAG+AGG 0.660756 6_3:-16571315 Msa0942190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAACATGTATGTTTATA+TGG - chr6_3:16570780-16570799 Msa0942190:CDS 20.0%
!! CAAAACATGTATGTTTATAT+GGG - chr6_3:16570781-16570800 Msa0942190:CDS 20.0%
!! AAATAAACTGCTTGTAAATT+TGG - chr6_3:16571224-16571243 Msa0942190:intron 20.0%
!! AATAAACTGCTTGTAAATTT+GGG - chr6_3:16571225-16571244 Msa0942190:intron 20.0%
!! ACAAAACATGTATGTTTATA+TGG - chr6_3:16570780-16570799 Msa0942190:CDS 20.0%
!! CAAAACATGTATGTTTATAT+GGG - chr6_3:16570781-16570800 Msa0942190:CDS 20.0%
!! AAATAAACTGCTTGTAAATT+TGG - chr6_3:16571224-16571243 Msa0942190:intron 20.0%
!! AATAAACTGCTTGTAAATTT+GGG - chr6_3:16571225-16571244 Msa0942190:intron 20.0%
! AAAGATCGTTTAAGTAACTT+TGG - chr6_3:16571019-16571038 Msa0942190:intron 25.0%
!! TAAGTAACTTTGGCTTAATA+TGG - chr6_3:16571029-16571048 Msa0942190:intron 25.0%
! ATTCTCATAAACAATATTGC+AGG - chr6_3:16571059-16571078 Msa0942190:intron 25.0%
! TTCAATACCATTTGATTGTT+TGG + chr6_3:16571277-16571296 None:intergenic 25.0%
! TTTGAAGAATGGAATTTCTA+AGG - chr6_3:16571317-16571336 Msa0942190:CDS 25.0%
! AAAGATCGTTTAAGTAACTT+TGG - chr6_3:16571019-16571038 Msa0942190:intron 25.0%
!! TAAGTAACTTTGGCTTAATA+TGG - chr6_3:16571029-16571048 Msa0942190:intron 25.0%
! ATTCTCATAAACAATATTGC+AGG - chr6_3:16571059-16571078 Msa0942190:intron 25.0%
! TTCAATACCATTTGATTGTT+TGG + chr6_3:16571277-16571296 None:intergenic 25.0%
! TTTGAAGAATGGAATTTCTA+AGG - chr6_3:16571317-16571336 Msa0942190:CDS 25.0%
AGCAAAAAATCTTCTTGTTC+TGG + chr6_3:16570643-16570662 None:intergenic 30.0%
TTGTAACAAATGAAAGACAG+CGG + chr6_3:16570764-16570783 None:intergenic 30.0%
!! ACAGTTGGTATACAACTTTT+TGG - chr6_3:16570816-16570835 Msa0942190:CDS 30.0%
TTCTTGCATATCCAGTATTA+TGG + chr6_3:16570865-16570884 None:intergenic 30.0%
! TCAGATTTTCCCATCTTTTT+GGG - chr6_3:16570993-16571012 Msa0942190:intron 30.0%
! ATCTTTTGACCCAAAAAGAT+GGG + chr6_3:16571005-16571024 None:intergenic 30.0%
! CTTTTCTTGAATGGAATGTA+TGG + chr6_3:16571436-16571455 None:intergenic 30.0%
AGCAAAAAATCTTCTTGTTC+TGG + chr6_3:16570643-16570662 None:intergenic 30.0%
TTGTAACAAATGAAAGACAG+CGG + chr6_3:16570764-16570783 None:intergenic 30.0%
!! ACAGTTGGTATACAACTTTT+TGG - chr6_3:16570816-16570835 Msa0942190:CDS 30.0%
TTCTTGCATATCCAGTATTA+TGG + chr6_3:16570865-16570884 None:intergenic 30.0%
! TCAGATTTTCCCATCTTTTT+GGG - chr6_3:16570993-16571012 Msa0942190:intron 30.0%
! ATCTTTTGACCCAAAAAGAT+GGG + chr6_3:16571005-16571024 None:intergenic 30.0%
! CTTTTCTTGAATGGAATGTA+TGG + chr6_3:16571436-16571455 None:intergenic 30.0%
AGAATAGAAGAGCAAGCAAA+AGG - chr6_3:16570606-16570625 Msa0942190:CDS 35.0%
AAGAACCTTGGATTTGTAAC+TGG + chr6_3:16570692-16570711 None:intergenic 35.0%
! AAATCCAAGGTTCTTTGAAG+AGG - chr6_3:16570697-16570716 Msa0942190:CDS 35.0%
GGGAAACTAAAATGTACAGT+TGG - chr6_3:16570801-16570820 Msa0942190:CDS 35.0%
AAAGCACATGCACTTGTAAT+AGG + chr6_3:16570842-16570861 None:intergenic 35.0%
! GTCAGATTTTCCCATCTTTT+TGG - chr6_3:16570992-16571011 Msa0942190:intron 35.0%
! GATCTTTTGACCCAAAAAGA+TGG + chr6_3:16571006-16571025 None:intergenic 35.0%
CATAAACAATATTGCAGGCA+TGG - chr6_3:16571064-16571083 Msa0942190:intron 35.0%
!!! GATCATCAGCTTTTAAAGCT+TGG + chr6_3:16571137-16571156 None:intergenic 35.0%
!! AGCTTTAAAAGCTGATGATC+AGG - chr6_3:16571137-16571156 Msa0942190:intron 35.0%
AGAGACTCCAAACAATCAAA+TGG - chr6_3:16571267-16571286 Msa0942190:intron 35.0%
! ATCAAATGGTATTGAACCCT+TGG - chr6_3:16571281-16571300 Msa0942190:intron 35.0%
! AAAGCATCTGAATCTTCCAA+GGG + chr6_3:16571300-16571319 None:intergenic 35.0%
!! GATTCAGATGCTTTGAAGAA+TGG - chr6_3:16571306-16571325 Msa0942190:CDS 35.0%
AGAATAGAAGAGCAAGCAAA+AGG - chr6_3:16570606-16570625 Msa0942190:CDS 35.0%
AAGAACCTTGGATTTGTAAC+TGG + chr6_3:16570692-16570711 None:intergenic 35.0%
! AAATCCAAGGTTCTTTGAAG+AGG - chr6_3:16570697-16570716 Msa0942190:CDS 35.0%
GGGAAACTAAAATGTACAGT+TGG - chr6_3:16570801-16570820 Msa0942190:CDS 35.0%
AAAGCACATGCACTTGTAAT+AGG + chr6_3:16570842-16570861 None:intergenic 35.0%
! GTCAGATTTTCCCATCTTTT+TGG - chr6_3:16570992-16571011 Msa0942190:intron 35.0%
! GATCTTTTGACCCAAAAAGA+TGG + chr6_3:16571006-16571025 None:intergenic 35.0%
CATAAACAATATTGCAGGCA+TGG - chr6_3:16571064-16571083 Msa0942190:intron 35.0%
!!! GATCATCAGCTTTTAAAGCT+TGG + chr6_3:16571137-16571156 None:intergenic 35.0%
!! AGCTTTAAAAGCTGATGATC+AGG - chr6_3:16571137-16571156 Msa0942190:intron 35.0%
AGAGACTCCAAACAATCAAA+TGG - chr6_3:16571267-16571286 Msa0942190:intron 35.0%
! ATCAAATGGTATTGAACCCT+TGG - chr6_3:16571281-16571300 Msa0942190:intron 35.0%
! AAAGCATCTGAATCTTCCAA+GGG + chr6_3:16571300-16571319 None:intergenic 35.0%
!! GATTCAGATGCTTTGAAGAA+TGG - chr6_3:16571306-16571325 Msa0942190:CDS 35.0%
GGATTTGTAACTGGAACTCA+CGG + chr6_3:16570683-16570702 None:intergenic 40.0%
GAGTTCCAGTTACAAATCCA+AGG - chr6_3:16570684-16570703 Msa0942190:CDS 40.0%
TTCTCCTCTTCAAAGAACCT+TGG + chr6_3:16570704-16570723 None:intergenic 40.0%
! CTTTGAAGAGGAGAAAACAG+AGG - chr6_3:16570709-16570728 Msa0942190:CDS 40.0%
AAAGACAGCGGAAGAAATAG+CGG + chr6_3:16570752-16570771 None:intergenic 40.0%
GCATGTGCTTTCCATAATAC+TGG - chr6_3:16570851-16570870 Msa0942190:CDS 40.0%
TTGTTACTGTCTGTCTCTCT+TGG - chr6_3:16570937-16570956 Msa0942190:CDS 40.0%
TGTTACTGTCTGTCTCTCTT+GGG - chr6_3:16570938-16570957 Msa0942190:CDS 40.0%
TCAGGAAGCTTACATGAGAA+TGG - chr6_3:16571155-16571174 Msa0942190:intron 40.0%
! CAAAGCATCTGAATCTTCCA+AGG + chr6_3:16571301-16571320 None:intergenic 40.0%
! TTCTAAGGAATCACCTCTTG+AGG - chr6_3:16571332-16571351 Msa0942190:CDS 40.0%
AAATCCTCATCTTCCTCAAG+AGG + chr6_3:16571348-16571367 None:intergenic 40.0%
TGAGGAAGATGAGGATTTGA+TGG - chr6_3:16571350-16571369 Msa0942190:CDS 40.0%
GACAGCAGTGATTTACTTGA+AGG - chr6_3:16571390-16571409 Msa0942190:CDS 40.0%
GTGATTTACTTGAAGGTCAG+CGG - chr6_3:16571397-16571416 Msa0942190:CDS 40.0%
GGATTTGTAACTGGAACTCA+CGG + chr6_3:16570683-16570702 None:intergenic 40.0%
GAGTTCCAGTTACAAATCCA+AGG - chr6_3:16570684-16570703 Msa0942190:CDS 40.0%
TTCTCCTCTTCAAAGAACCT+TGG + chr6_3:16570704-16570723 None:intergenic 40.0%
! CTTTGAAGAGGAGAAAACAG+AGG - chr6_3:16570709-16570728 Msa0942190:CDS 40.0%
AAAGACAGCGGAAGAAATAG+CGG + chr6_3:16570752-16570771 None:intergenic 40.0%
GCATGTGCTTTCCATAATAC+TGG - chr6_3:16570851-16570870 Msa0942190:CDS 40.0%
TTGTTACTGTCTGTCTCTCT+TGG - chr6_3:16570937-16570956 Msa0942190:CDS 40.0%
TGTTACTGTCTGTCTCTCTT+GGG - chr6_3:16570938-16570957 Msa0942190:CDS 40.0%
TCAGGAAGCTTACATGAGAA+TGG - chr6_3:16571155-16571174 Msa0942190:intron 40.0%
! CAAAGCATCTGAATCTTCCA+AGG + chr6_3:16571301-16571320 None:intergenic 40.0%
! TTCTAAGGAATCACCTCTTG+AGG - chr6_3:16571332-16571351 Msa0942190:CDS 40.0%
AAATCCTCATCTTCCTCAAG+AGG + chr6_3:16571348-16571367 None:intergenic 40.0%
TGAGGAAGATGAGGATTTGA+TGG - chr6_3:16571350-16571369 Msa0942190:CDS 40.0%
GACAGCAGTGATTTACTTGA+AGG - chr6_3:16571390-16571409 Msa0942190:CDS 40.0%
GTGATTTACTTGAAGGTCAG+CGG - chr6_3:16571397-16571416 Msa0942190:CDS 40.0%
ACAGAGGAATGATGCTGTTC+AGG - chr6_3:16570725-16570744 Msa0942190:CDS 45.0%
ACAATATTGCAGGCATGGCA+TGG - chr6_3:16571069-16571088 Msa0942190:intron 45.0%
GCTTGTAAATTTGGGAGCAG+CGG - chr6_3:16571233-16571252 Msa0942190:intron 45.0%
ATCACCTCTTGAGGAAGATG+AGG - chr6_3:16571341-16571360 Msa0942190:CDS 45.0%
ACAGAGGAATGATGCTGTTC+AGG - chr6_3:16570725-16570744 Msa0942190:CDS 45.0%
ACAATATTGCAGGCATGGCA+TGG - chr6_3:16571069-16571088 Msa0942190:intron 45.0%
GCTTGTAAATTTGGGAGCAG+CGG - chr6_3:16571233-16571252 Msa0942190:intron 45.0%
ATCACCTCTTGAGGAAGATG+AGG - chr6_3:16571341-16571360 Msa0942190:CDS 45.0%
TATTGCAGGCATGGCATGGA+AGG - chr6_3:16571073-16571092 Msa0942190:intron 50.0%
GGCAAAGACAACGTGCAACT+CGG - chr6_3:16571094-16571113 Msa0942190:intron 50.0%
GCAAAGACAACGTGCAACTC+GGG - chr6_3:16571095-16571114 Msa0942190:intron 50.0%
TATTGCAGGCATGGCATGGA+AGG - chr6_3:16571073-16571092 Msa0942190:intron 50.0%
GGCAAAGACAACGTGCAACT+CGG - chr6_3:16571094-16571113 Msa0942190:intron 50.0%
GCAAAGACAACGTGCAACTC+GGG - chr6_3:16571095-16571114 Msa0942190:intron 50.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 16570591 16571455 16570591 ID=Msa0942190;Name=Msa0942190
chr6_3 mRNA 16570591 16571455 16570591 ID=Msa0942190-mRNA-1;Parent=Msa0942190;Name=Msa0942190-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|1|1|2|0|177
chr6_3 exon 16571300 16571455 16571300 ID=Msa0942190-mRNA-1:exon:5041;Parent=Msa0942190-mRNA-1
chr6_3 exon 16570591 16570965 16570591 ID=Msa0942190-mRNA-1:exon:5040;Parent=Msa0942190-mRNA-1
chr6_3 CDS 16571300 16571455 16571300 ID=Msa0942190-mRNA-1:cds;Parent=Msa0942190-mRNA-1
chr6_3 CDS 16570591 16570965 16570591 ID=Msa0942190-mRNA-1:cds;Parent=Msa0942190-mRNA-1
Gene Sequence

>Msa0942190

TTGCAGAGACTATCTAGAATAGAAGAGCAAGCAAAAGGTCAGATAGAGACCAGAACAAGAAGATTTTTTGCTGAAATACTGAATGCTGTCCGTGAGTTCCAGTTACAAATCCAAGGTTCTTTGAAGAGGAGAAAACAGAGGAATGATGCTGTTCAGGCATGGCATGGAAGGCAAAGACAACGTGCAACTCGGGCTGAGAAGCTGAGATTCCAAGCTTTAAAAGCTGATGATCAGGAAGCTTACATGAGAATGGTGAAAGAGAGTAAGAATGAAAGATTAACGTTGCTTCTTGAAGAAACAAATAAACTGCTTGTAAATTTGGGAGCAGCGGTTCAACGTCAAAGAGACTCCAAACAATCAAATGGTATTGAACCCTTGGAAGATTCAGATGCTTTGAAGAATGGAATTTCTAAGGAATCACCTCTTGAGGAAGATGAGGATTTGATGGATTCTGATCATAATGATGACAGCAGTGATTTACTTGAAGGTCAGCGGCAATACAATTCAACCATACATTCCATTCAAGAAAAG

Protein sequence

>Msa0942190

LQRLSRIEEQAKGQIETRTRRFFAEILNAVREFQLQIQGSLKRRKQRNDAVQAWHGRQRQRATRAEKLRFQALKADDQEAYMRMVKESKNERLTLLLEETNKLLVNLGAAVQRQRDSKQSNGIEPLEDSDALKNGISKESPLEEDEDLMDSDHNDDSSDLLEGQRQYNSTIHSIQEK