AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0387250


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGAAAAGATGGTCATAAT+TGG 0.253911 3_1:+91157796 Msa0387250:CDS
GAAGTTGAACATTTGCGTTT+TGG 0.335077 3_1:-91156656 None:intergenic
GGGAAAATCGGTGAACTTGC+AGG 0.359647 3_1:-91157597 None:intergenic
TGTTACCATGAGGCTGGTTA+AGG 0.382153 3_1:-91157619 None:intergenic
AGAAGCGCATGAACGGCTTA+AGG 0.406373 3_1:+91157848 Msa0387250:CDS
ATGCGTTTCTTTCGAAAAGA+TGG 0.423560 3_1:+91157786 Msa0387250:CDS
AAAATGTAAAGCACTTCTAT+AGG 0.441324 3_1:-91157560 None:intergenic
TTCAAGAAGCTAAGAAAAGA+TGG 0.482062 3_1:+91157531 Msa0387250:five_prime_UTR
GTTACCATGAGGCTGGTTAA+GGG 0.482952 3_1:-91157618 None:intergenic
AGGCTGGTTAAGGGGAAAAT+CGG 0.489276 3_1:-91157609 None:intergenic
GAAAAGATGGTCATAATTGG+CGG 0.506642 3_1:+91157799 Msa0387250:CDS
CTGTTTCAGAAGCGCATGAA+CGG 0.548045 3_1:+91157841 Msa0387250:CDS
TAAGAATGTTACCATGAGGC+TGG 0.551040 3_1:-91157625 None:intergenic
TTTCCCCTTAACCAGCCTCA+TGG 0.561765 3_1:+91157614 Msa0387250:CDS
AGATCAATTACGATGCTACC+TGG 0.572627 3_1:+91156692 Msa0387250:five_prime_UTR
GGTAGCATCGTAATTGATCT+CGG 0.575675 3_1:-91156689 None:intergenic
TTACCATGAGGCTGGTTAAG+GGG 0.590627 3_1:-91157617 None:intergenic
CATCGTAATTGATCTCGGAA+TGG 0.597806 3_1:-91156684 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAAAATTGAATTGATTAA+AGG - chr3_1:91156767-91156786 None:intergenic 15.0%
!!! AGTTCTAATTTCTAAATTAT+TGG + chr3_1:91157457-91157476 Msa0387250:intron 15.0%
!!! GCAATTGATTTTTTTTTTAA+AGG + chr3_1:91157732-91157751 Msa0387250:intron 15.0%
!!! ATTGATTTTTTTTTTAAAGG+TGG + chr3_1:91157735-91157754 Msa0387250:intron 15.0%
!! AGTAAAATTGAATTGATTAA+AGG - chr3_1:91156767-91156786 None:intergenic 15.0%
!!! AGTTCTAATTTCTAAATTAT+TGG + chr3_1:91157457-91157476 Msa0387250:intron 15.0%
!!! GCAATTGATTTTTTTTTTAA+AGG + chr3_1:91157732-91157751 Msa0387250:intron 15.0%
!!! ATTGATTTTTTTTTTAAAGG+TGG + chr3_1:91157735-91157754 Msa0387250:intron 15.0%
!! AAAAATGAAAATTATGCAGA+AGG - chr3_1:91156730-91156749 None:intergenic 20.0%
!!! GTTTTTTTGTGTGTTATTAT+TGG + chr3_1:91156857-91156876 Msa0387250:intron 20.0%
!! GAACTGTAAAAATATCAAAA+AGG - chr3_1:91156890-91156909 None:intergenic 20.0%
!! AAAAATGAAAATTATGCAGA+AGG - chr3_1:91156730-91156749 None:intergenic 20.0%
!!! GTTTTTTTGTGTGTTATTAT+TGG + chr3_1:91156857-91156876 Msa0387250:intron 20.0%
!! GAACTGTAAAAATATCAAAA+AGG - chr3_1:91156890-91156909 None:intergenic 20.0%
! AATTGAATTGATTAAAGGAG+AGG - chr3_1:91156762-91156781 None:intergenic 25.0%
! TTGAATTCAAATTCAGTTCA+TGG + chr3_1:91156814-91156833 Msa0387250:intron 25.0%
! TTGTTTATCGTTGTGTTAAA+AGG - chr3_1:91157014-91157033 None:intergenic 25.0%
! TTGCATCTTGTATAAAAAAC+AGG + chr3_1:91157132-91157151 Msa0387250:intron 25.0%
! TGCATCTTGTATAAAAAACA+GGG + chr3_1:91157133-91157152 Msa0387250:intron 25.0%
!! AAAATGTAAAGCACTTCTAT+AGG - chr3_1:91157563-91157582 None:intergenic 25.0%
! AATTGAATTGATTAAAGGAG+AGG - chr3_1:91156762-91156781 None:intergenic 25.0%
! TTGAATTCAAATTCAGTTCA+TGG + chr3_1:91156814-91156833 Msa0387250:intron 25.0%
! TTGTTTATCGTTGTGTTAAA+AGG - chr3_1:91157014-91157033 None:intergenic 25.0%
! TTGCATCTTGTATAAAAAAC+AGG + chr3_1:91157132-91157151 Msa0387250:intron 25.0%
! TGCATCTTGTATAAAAAACA+GGG + chr3_1:91157133-91157152 Msa0387250:intron 25.0%
!! AAAATGTAAAGCACTTCTAT+AGG - chr3_1:91157563-91157582 None:intergenic 25.0%
TAACACAACGATAAACAAGT+CGG + chr3_1:91157016-91157035 Msa0387250:intron 30.0%
AAATGTCATGAGTTAAATCC+TGG + chr3_1:91157106-91157125 Msa0387250:intron 30.0%
TTATACAAGATGCAATTTCC+AGG - chr3_1:91157127-91157146 None:intergenic 30.0%
ACAAACATTCACTTAGTGTA+TGG - chr3_1:91157215-91157234 None:intergenic 30.0%
! ATGATGGTTTGTCAGAATAA+CGG - chr3_1:91157327-91157346 None:intergenic 30.0%
AACTCGGAATTCCATAAAAA+TGG + chr3_1:91157408-91157427 Msa0387250:intron 30.0%
!! CCTTTTCATCTCCATTTTTA+TGG - chr3_1:91157422-91157441 None:intergenic 30.0%
CCATAAAAATGGAGATGAAA+AGG + chr3_1:91157419-91157438 Msa0387250:intron 30.0%
TTCAAGAAGCTAAGAAAAGA+TGG + chr3_1:91157531-91157550 Msa0387250:five_prime_UTR 30.0%
AGAATAAGAATGTTACCATG+AGG - chr3_1:91157632-91157651 None:intergenic 30.0%
! AATTAAGTGACCTGATTTTC+TGG + chr3_1:91157673-91157692 Msa0387250:intron 30.0%
! TTACTGATTGTCAACAACAT+TGG + chr3_1:91157710-91157729 Msa0387250:intron 30.0%
TTCGAAAAGATGGTCATAAT+TGG + chr3_1:91157796-91157815 Msa0387250:CDS 30.0%
TAACACAACGATAAACAAGT+CGG + chr3_1:91157016-91157035 Msa0387250:intron 30.0%
AAATGTCATGAGTTAAATCC+TGG + chr3_1:91157106-91157125 Msa0387250:intron 30.0%
TTATACAAGATGCAATTTCC+AGG - chr3_1:91157127-91157146 None:intergenic 30.0%
ACAAACATTCACTTAGTGTA+TGG - chr3_1:91157215-91157234 None:intergenic 30.0%
! ATGATGGTTTGTCAGAATAA+CGG - chr3_1:91157327-91157346 None:intergenic 30.0%
AACTCGGAATTCCATAAAAA+TGG + chr3_1:91157408-91157427 Msa0387250:intron 30.0%
!! CCTTTTCATCTCCATTTTTA+TGG - chr3_1:91157422-91157441 None:intergenic 30.0%
CCATAAAAATGGAGATGAAA+AGG + chr3_1:91157419-91157438 Msa0387250:intron 30.0%
TTCAAGAAGCTAAGAAAAGA+TGG + chr3_1:91157531-91157550 Msa0387250:five_prime_UTR 30.0%
AGAATAAGAATGTTACCATG+AGG - chr3_1:91157632-91157651 None:intergenic 30.0%
! AATTAAGTGACCTGATTTTC+TGG + chr3_1:91157673-91157692 Msa0387250:intron 30.0%
! TTACTGATTGTCAACAACAT+TGG + chr3_1:91157710-91157729 Msa0387250:intron 30.0%
TTCGAAAAGATGGTCATAAT+TGG + chr3_1:91157796-91157815 Msa0387250:CDS 30.0%
! GAAGTTGAACATTTGCGTTT+TGG - chr3_1:91156659-91156678 None:intergenic 35.0%
! AGAAGGAGAAAGTTTTTACC+AGG - chr3_1:91156713-91156732 None:intergenic 35.0%
! TGACTTGACTGGTCATAAAA+AGG - chr3_1:91156921-91156940 None:intergenic 35.0%
CTGTGTACAATACACCAAAA+TGG + chr3_1:91157161-91157180 Msa0387250:intron 35.0%
TGTACAATACACCAAAATGG+TGG + chr3_1:91157164-91157183 Msa0387250:intron 35.0%
GTACAATACACCAAAATGGT+GGG + chr3_1:91157165-91157184 Msa0387250:intron 35.0%
! TAGCTTTTGCTAAATCACAG+TGG - chr3_1:91157270-91157289 None:intergenic 35.0%
! TAGCTTTTGCAAAATCACAG+TGG + chr3_1:91157297-91157316 Msa0387250:intron 35.0%
!! ATTCCGAGTTGATCAAAGTT+GGG - chr3_1:91157398-91157417 None:intergenic 35.0%
!! AATTCCGAGTTGATCAAAGT+TGG - chr3_1:91157399-91157418 None:intergenic 35.0%
TAACTTGACATTGTCATGAC+AGG + chr3_1:91157482-91157501 Msa0387250:intron 35.0%
ATGCGTTTCTTTCGAAAAGA+TGG + chr3_1:91157786-91157805 Msa0387250:CDS 35.0%
GAAAAGATGGTCATAATTGG+CGG + chr3_1:91157799-91157818 Msa0387250:CDS 35.0%
AATTGGCGGAAGAAAAAAGA+TGG + chr3_1:91157813-91157832 Msa0387250:CDS 35.0%
! GAAGTTGAACATTTGCGTTT+TGG - chr3_1:91156659-91156678 None:intergenic 35.0%
! AGAAGGAGAAAGTTTTTACC+AGG - chr3_1:91156713-91156732 None:intergenic 35.0%
! TGACTTGACTGGTCATAAAA+AGG - chr3_1:91156921-91156940 None:intergenic 35.0%
CTGTGTACAATACACCAAAA+TGG + chr3_1:91157161-91157180 Msa0387250:intron 35.0%
TGTACAATACACCAAAATGG+TGG + chr3_1:91157164-91157183 Msa0387250:intron 35.0%
GTACAATACACCAAAATGGT+GGG + chr3_1:91157165-91157184 Msa0387250:intron 35.0%
! TAGCTTTTGCTAAATCACAG+TGG - chr3_1:91157270-91157289 None:intergenic 35.0%
! TAGCTTTTGCAAAATCACAG+TGG + chr3_1:91157297-91157316 Msa0387250:intron 35.0%
!! ATTCCGAGTTGATCAAAGTT+GGG - chr3_1:91157398-91157417 None:intergenic 35.0%
!! AATTCCGAGTTGATCAAAGT+TGG - chr3_1:91157399-91157418 None:intergenic 35.0%
TAACTTGACATTGTCATGAC+AGG + chr3_1:91157482-91157501 Msa0387250:intron 35.0%
ATGCGTTTCTTTCGAAAAGA+TGG + chr3_1:91157786-91157805 Msa0387250:CDS 35.0%
GAAAAGATGGTCATAATTGG+CGG + chr3_1:91157799-91157818 Msa0387250:CDS 35.0%
AATTGGCGGAAGAAAAAAGA+TGG + chr3_1:91157813-91157832 Msa0387250:CDS 35.0%
CATCGTAATTGATCTCGGAA+TGG - chr3_1:91156687-91156706 None:intergenic 40.0%
GGTAGCATCGTAATTGATCT+CGG - chr3_1:91156692-91156711 None:intergenic 40.0%
AGATCAATTACGATGCTACC+TGG + chr3_1:91156692-91156711 Msa0387250:five_prime_UTR 40.0%
!! ACAATCAGAACTGACTTGAC+TGG - chr3_1:91156932-91156951 None:intergenic 40.0%
GCTCATGTTAGACATCATGA+TGG - chr3_1:91157343-91157362 None:intergenic 40.0%
!! TTCCGAGTTGATCAAAGTTG+GGG - chr3_1:91157397-91157416 None:intergenic 40.0%
TAAGAATGTTACCATGAGGC+TGG - chr3_1:91157628-91157647 None:intergenic 40.0%
GCACAAATCACCAGAAAATC+AGG - chr3_1:91157686-91157705 None:intergenic 40.0%
CATCGTAATTGATCTCGGAA+TGG - chr3_1:91156687-91156706 None:intergenic 40.0%
GGTAGCATCGTAATTGATCT+CGG - chr3_1:91156692-91156711 None:intergenic 40.0%
AGATCAATTACGATGCTACC+TGG + chr3_1:91156692-91156711 Msa0387250:five_prime_UTR 40.0%
!! ACAATCAGAACTGACTTGAC+TGG - chr3_1:91156932-91156951 None:intergenic 40.0%
GCTCATGTTAGACATCATGA+TGG - chr3_1:91157343-91157362 None:intergenic 40.0%
!! TTCCGAGTTGATCAAAGTTG+GGG - chr3_1:91157397-91157416 None:intergenic 40.0%
TAAGAATGTTACCATGAGGC+TGG - chr3_1:91157628-91157647 None:intergenic 40.0%
GCACAAATCACCAGAAAATC+AGG - chr3_1:91157686-91157705 None:intergenic 40.0%
ACAGAGTTTGTGCCACGTAT+GGG + chr3_1:91157043-91157062 Msa0387250:intron 45.0%
GTATGGTATACGCACAGTTC+TGG - chr3_1:91157198-91157217 None:intergenic 45.0%
! AGGCTGGTTAAGGGGAAAAT+CGG - chr3_1:91157612-91157631 None:intergenic 45.0%
! TTACCATGAGGCTGGTTAAG+GGG - chr3_1:91157620-91157639 None:intergenic 45.0%
! GTTACCATGAGGCTGGTTAA+GGG - chr3_1:91157621-91157640 None:intergenic 45.0%
! TGTTACCATGAGGCTGGTTA+AGG - chr3_1:91157622-91157641 None:intergenic 45.0%
! CTGTTTCAGAAGCGCATGAA+CGG + chr3_1:91157841-91157860 Msa0387250:CDS 45.0%
ACAGAGTTTGTGCCACGTAT+GGG + chr3_1:91157043-91157062 Msa0387250:intron 45.0%
GTATGGTATACGCACAGTTC+TGG - chr3_1:91157198-91157217 None:intergenic 45.0%
! AGGCTGGTTAAGGGGAAAAT+CGG - chr3_1:91157612-91157631 None:intergenic 45.0%
! TTACCATGAGGCTGGTTAAG+GGG - chr3_1:91157620-91157639 None:intergenic 45.0%
! GTTACCATGAGGCTGGTTAA+GGG - chr3_1:91157621-91157640 None:intergenic 45.0%
! TGTTACCATGAGGCTGGTTA+AGG - chr3_1:91157622-91157641 None:intergenic 45.0%
! CTGTTTCAGAAGCGCATGAA+CGG + chr3_1:91157841-91157860 Msa0387250:CDS 45.0%
GACAGAGTTTGTGCCACGTA+TGG + chr3_1:91157042-91157061 Msa0387250:intron 50.0%
CAGAGTTTGTGCCACGTATG+GGG + chr3_1:91157044-91157063 Msa0387250:intron 50.0%
AGAGTTTGTGCCACGTATGG+GGG + chr3_1:91157045-91157064 Msa0387250:intron 50.0%
CGTTAAAACTCCCCCATACG+TGG - chr3_1:91157058-91157077 None:intergenic 50.0%
! TGGAAGGAGTCCCACCATTT+TGG - chr3_1:91157178-91157197 None:intergenic 50.0%
!! GGTATACGCACAGTTCTGGA+AGG - chr3_1:91157194-91157213 None:intergenic 50.0%
TAGCCAGTAGTCACAGTGTG+TGG + chr3_1:91157371-91157390 Msa0387250:intron 50.0%
GGCCCCAACTTTGATCAACT+CGG + chr3_1:91157392-91157411 Msa0387250:intron 50.0%
GGGAAAATCGGTGAACTTGC+AGG - chr3_1:91157600-91157619 None:intergenic 50.0%
TTTCCCCTTAACCAGCCTCA+TGG + chr3_1:91157614-91157633 Msa0387250:CDS 50.0%
GACAGAGTTTGTGCCACGTA+TGG + chr3_1:91157042-91157061 Msa0387250:intron 50.0%
CAGAGTTTGTGCCACGTATG+GGG + chr3_1:91157044-91157063 Msa0387250:intron 50.0%
AGAGTTTGTGCCACGTATGG+GGG + chr3_1:91157045-91157064 Msa0387250:intron 50.0%
CGTTAAAACTCCCCCATACG+TGG - chr3_1:91157058-91157077 None:intergenic 50.0%
! TGGAAGGAGTCCCACCATTT+TGG - chr3_1:91157178-91157197 None:intergenic 50.0%
!! GGTATACGCACAGTTCTGGA+AGG - chr3_1:91157194-91157213 None:intergenic 50.0%
TAGCCAGTAGTCACAGTGTG+TGG + chr3_1:91157371-91157390 Msa0387250:intron 50.0%
GGCCCCAACTTTGATCAACT+CGG + chr3_1:91157392-91157411 Msa0387250:intron 50.0%
GGGAAAATCGGTGAACTTGC+AGG - chr3_1:91157600-91157619 None:intergenic 50.0%
TTTCCCCTTAACCAGCCTCA+TGG + chr3_1:91157614-91157633 Msa0387250:CDS 50.0%
GGGCCACACACTGTGACTAC+TGG - chr3_1:91157377-91157396 None:intergenic 60.0%
GGGCCACACACTGTGACTAC+TGG - chr3_1:91157377-91157396 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 91156578 91157867 91156578 ID=Msa0387250;Name=Msa0387250
chr3_1 mRNA 91156578 91157867 91156578 ID=Msa0387250-mRNA-1;Parent=Msa0387250;Name=Msa0387250-mRNA-1;_AED=0.15;_eAED=0.15;_QI=223|0.5|0.33|0.66|0.5|0|3|0|53
chr3_1 exon 91156578 91156713 91156578 ID=Msa0387250-mRNA-1:exon:25318;Parent=Msa0387250-mRNA-1
chr3_1 exon 91157504 91157635 91157504 ID=Msa0387250-mRNA-1:exon:25319;Parent=Msa0387250-mRNA-1
chr3_1 exon 91157754 91157867 91157754 ID=Msa0387250-mRNA-1:exon:25320;Parent=Msa0387250-mRNA-1
chr3_1 five_prime_UTR 91156578 91156713 91156578 ID=Msa0387250-mRNA-1:five_prime_utr;Parent=Msa0387250-mRNA-1
chr3_1 five_prime_UTR 91157504 91157590 91157504 ID=Msa0387250-mRNA-1:five_prime_utr;Parent=Msa0387250-mRNA-1
chr3_1 CDS 91157591 91157635 91157591 ID=Msa0387250-mRNA-1:cds;Parent=Msa0387250-mRNA-1
chr3_1 CDS 91157754 91157867 91157754 ID=Msa0387250-mRNA-1:cds;Parent=Msa0387250-mRNA-1
Gene Sequence

>Msa0387250

ATGATACCTGCAAGTTCACCGATTTTCCCCTTAACCAGCCTCATGGTGGATCTTTGTATCTGTTTAATAGAAGAGTCATGCGTTTCTTTCGAAAAGATGGTCATAATTGGCGGAAGAAAAAAGATGGAAGAACTGTTTCAGAAGCGCATGAACGGCTTA

Protein sequence

>Msa0387250

MIPASSPIFPLTSLMVDLCICLIEESCVSFEKMVIIGGRKKMEELFQKRMNGL