AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0828920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0828920 MtrunA17_Chr8g0380791 97.133 593 17 0 1 593 1 593 0.0 1199
Msa0828920 MtrunA17_Chr1g0180261 52.053 609 270 3 6 593 44 651 0.0 623
Msa0828920 MtrunA17_Chr8g0353121 41.818 605 307 11 2 592 41 614 3.73e-155 463
Msa0828920 MtrunA17_Chr1g0171491 40.163 615 321 8 9 591 57 656 5.81e-155 464
Msa0828920 MtrunA17_Chr1g0171491 37.815 119 74 0 76 194 55 173 3.53e-18 88.6
Msa0828920 MtrunA17_Chr7g0256301 41.528 602 330 10 8 592 66 662 4.95e-149 449
Msa0828920 MtrunA17_Chr3g0115111 41.304 598 283 12 1 591 45 581 7.85e-147 441
Msa0828920 MtrunA17_Chr3g0082501 38.799 616 292 13 9 591 60 623 4.26e-134 409
Msa0828920 MtrunA17_Chr3g0082501 28.643 199 73 2 76 205 58 256 5.50e-17 84.7
Msa0828920 MtrunA17_Chr3g0131001 40.410 537 292 10 59 591 55 567 9.84e-116 360
Msa0828920 MtrunA17_Chr3g0131001 43.500 200 113 0 9 208 51 250 4.39e-44 167
Msa0828920 MtrunA17_Chr3g0131001 41.176 85 50 0 7 91 141 225 8.30e-13 71.2
Msa0828920 MtrunA17_Chr5g0424301 58.103 253 103 2 341 591 331 582 5.52e-99 317
Msa0828920 MtrunA17_Chr5g0424301 46.429 168 90 0 9 176 65 232 5.07e-48 179
Msa0828920 MtrunA17_Chr5g0424301 35.135 148 94 1 47 194 59 204 2.02e-21 99.0
Msa0828920 MtrunA17_Chr8g0385201 55.805 267 114 3 327 591 277 541 3.46e-97 310
Msa0828920 MtrunA17_Chr8g0385201 45.509 167 90 1 6 171 23 189 3.57e-43 164
Msa0828920 MtrunA17_Chr8g0385201 37.956 137 84 1 59 194 30 166 3.73e-19 91.7
Msa0828920 MtrunA17_Chr8g0385201 44.595 74 41 0 7 80 117 190 3.27e-13 72.8
Msa0828920 MtrunA17_Chr8g0382971 41.209 364 146 8 6 337 18 345 5.31e-67 222
Msa0828920 MtrunA17_Chr8g0377671 54.167 192 88 0 1 192 8 199 1.19e-63 211
Msa0828920 MtrunA17_Chr8g0377671 40.252 159 95 0 12 170 111 269 1.61e-30 121
Msa0828920 MtrunA17_Chr8g0385241 34.670 424 178 5 5 334 2 420 8.99e-59 202
Msa0828920 MtrunA17_Chr8g0377191 53.226 186 87 0 7 192 6 191 8.05e-58 192
Msa0828920 MtrunA17_Chr8g0377191 37.398 123 53 2 101 199 8 130 2.89e-13 68.9
Msa0828920 MtrunA17_Chr4g0059911 53.723 188 86 1 7 193 3 190 2.58e-57 197
Msa0828920 MtrunA17_Chr4g0059911 37.931 145 90 0 15 159 104 248 5.56e-21 95.1
Msa0828920 MtrunA17_Chr8g0377221 42.969 256 102 5 12 227 4 255 4.45e-53 182
Msa0828920 MtrunA17_Chr8g0383551 34.933 375 151 11 13 337 28 359 8.17e-49 174
Msa0828920 MtrunA17_Chr2g0316611 47.179 195 102 1 6 199 29 223 5.70e-45 163
Msa0828920 MtrunA17_Chr2g0316611 46.316 190 101 1 11 199 172 361 8.22e-42 155
Msa0828920 MtrunA17_Chr2g0316611 42.021 188 108 1 13 199 105 292 5.41e-38 144
Msa0828920 MtrunA17_Chr2g0316611 53.543 127 59 0 13 139 243 369 3.25e-35 136
Msa0828920 MtrunA17_Chr2g0316611 56.410 78 26 1 5 74 296 373 7.65e-18 85.9
Msa0828920 MtrunA17_Chr2g0316631 39.167 240 99 2 6 198 29 268 2.42e-43 157
Msa0828920 MtrunA17_Chr2g0316631 43.085 188 106 1 13 199 105 292 7.29e-40 147
Msa0828920 MtrunA17_Chr2g0316631 43.902 123 69 0 13 135 174 296 1.99e-26 109
Msa0828920 MtrunA17_Chr2g0316631 64.062 64 23 0 11 74 241 304 1.03e-19 90.1
Msa0828920 MtrunA17_Chr6g0467061 45.078 193 105 1 6 197 12 204 6.03e-43 157
Msa0828920 MtrunA17_Chr6g0467061 45.455 187 101 1 13 198 88 274 8.97e-40 148
Msa0828920 MtrunA17_Chr6g0467061 43.575 179 101 0 13 191 157 335 3.72e-34 132
Msa0828920 MtrunA17_Chr8g0377211 45.294 170 69 2 1 170 8 153 2.63e-40 144
Msa0828920 MtrunA17_Chr8g0377211 52.252 111 52 1 82 192 20 129 1.05e-28 112
Msa0828920 MtrunA17_Chr5g0398011 53.509 114 53 0 14 127 36 149 8.27e-32 120
Msa0828920 MtrunA17_Chr5g0398011 33.333 114 76 0 56 169 32 145 1.69e-14 71.2
Msa0828920 MtrunA17_Chr5g0398011 63.830 47 17 0 13 59 104 150 3.33e-14 70.5
Msa0828920 MtrunA17_Chr7g0233351 36.170 188 117 1 12 196 191 378 3.22e-30 124
Msa0828920 MtrunA17_Chr7g0233351 35.075 134 87 0 10 143 235 368 1.79e-18 88.6
Msa0828920 MtrunA17_Chr7g0233351 41.304 92 54 0 102 193 189 280 3.74e-14 75.1
Msa0828920 MtrunA17_Chr7g0233351 25.000 228 146 3 51 253 184 411 9.64e-14 73.6
Msa0828920 MtrunA17_Chr8g0383331 44.094 127 71 0 56 182 1 127 7.15e-29 112
Msa0828920 MtrunA17_Chr8g0383331 36.913 149 94 0 11 159 2 150 7.40e-24 98.6
Msa0828920 MtrunA17_Chr8g0383331 40.659 91 31 1 125 192 1 91 2.08e-13 68.6
Msa0828920 MtrunA17_Chr4g0055371 35.955 178 114 0 12 189 171 348 1.85e-28 118
Msa0828920 MtrunA17_Chr4g0055371 35.036 137 89 0 7 143 212 348 1.95e-18 88.2
Msa0828920 MtrunA17_Chr4g0055371 39.474 114 69 0 82 195 172 285 6.80e-14 73.9
Msa0828920 MtrunA17_Chr3g0114791 34.857 175 114 0 12 186 184 358 3.12e-28 117
Msa0828920 MtrunA17_Chr3g0114791 35.965 114 73 0 82 195 185 298 2.29e-12 69.3
Msa0828920 MtrunA17_Chr3g0114791 39.785 93 56 0 100 192 180 272 6.46e-12 67.8
Msa0828920 MtrunA17_Chr5g0424111 36.313 179 114 0 8 186 192 370 1.05e-27 116
Msa0828920 MtrunA17_Chr5g0424111 36.090 133 85 0 10 142 240 372 2.18e-16 82.0
Msa0828920 MtrunA17_Chr5g0424111 37.719 114 71 0 82 195 197 310 1.67e-12 69.7
Msa0828920 MtrunA17_Chr5g0398021 48.649 111 55 1 4 112 2 112 2.10e-27 108
Msa0828920 MtrunA17_Chr5g0398021 35.965 114 73 0 71 184 2 115 1.62e-14 71.2
Msa0828920 MtrunA17_Chr5g0398021 34.694 98 64 0 61 158 15 112 1.43e-12 65.9
Msa0828920 MtrunA17_Chr5g0398021 31.373 102 70 0 93 194 1 102 7.96e-11 60.8
Msa0828920 MtrunA17_Chr8g0336161 32.778 180 121 0 10 189 194 373 1.65e-24 107
Msa0828920 MtrunA17_Chr8g0336161 34.307 137 90 0 7 143 237 373 6.83e-18 86.7
Msa0828920 MtrunA17_Chr8g0336161 43.478 92 52 0 102 193 194 285 1.00e-14 77.0
Msa0828920 MtrunA17_Chr4g0069951 33.133 166 95 6 437 588 1552 1715 7.56e-15 78.6
Msa0828920 MtrunA17_Chr1g0200881 34.356 163 91 7 440 588 1346 1506 3.98e-14 76.3
Msa0828920 MtrunA17_Chr8g0345151 32.530 166 96 6 437 588 1558 1721 1.50e-13 74.3
Msa0828920 MtrunA17_Chr8g0377201 59.184 49 19 1 12 60 26 73 1.95e-12 63.2
Msa0828920 MtrunA17_Chr8g0377201 59.574 47 19 0 148 194 1 47 1.73e-11 60.5
Msa0828920 MtrunA17_Chr3g0085921 52.830 53 25 0 142 194 1 53 1.02e-11 62.0
Msa0828920 MtrunA17_Chr1g0210831 29.134 127 90 0 61 187 615 741 4.33e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0828920 AT1G60890.1 61.960 602 206 4 6 593 4 596 0.0 765
Msa0828920 AT1G60890.2 62.104 599 204 4 9 593 19 608 0.0 764
Msa0828920 AT1G10900.2 63.265 588 208 3 6 593 4 583 0.0 763
Msa0828920 AT1G10900.1 63.265 588 208 3 6 593 4 583 0.0 763
Msa0828920 AT1G10900.3 63.265 588 208 3 6 593 4 583 0.0 763
Msa0828920 AT3G09920.3 50.498 602 282 7 6 593 46 645 0.0 597
Msa0828920 AT3G09920.4 50.498 602 282 7 6 593 46 645 0.0 597
Msa0828920 AT3G09920.2 50.498 602 282 7 6 593 46 645 0.0 597
Msa0828920 AT3G09920.1 50.498 602 282 7 6 593 46 645 0.0 597
Msa0828920 AT3G07960.3 42.691 602 283 8 9 591 23 581 4.17e-166 487
Msa0828920 AT3G07960.3 36.975 119 75 0 76 194 21 139 1.01e-18 90.5
Msa0828920 AT3G07960.2 42.691 602 283 8 9 591 23 581 5.94e-166 486
Msa0828920 AT3G07960.2 36.975 119 75 0 76 194 21 139 1.32e-18 90.1
Msa0828920 AT3G07960.4 42.691 602 283 8 9 591 23 581 5.94e-166 486
Msa0828920 AT3G07960.4 36.975 119 75 0 76 194 21 139 1.32e-18 90.1
Msa0828920 AT3G07960.1 42.691 602 283 8 9 591 23 581 6.82e-165 487
Msa0828920 AT3G07960.1 36.975 119 75 0 76 194 21 139 6.55e-19 91.3
Msa0828920 AT2G41210.1 43.339 593 303 8 9 591 66 635 3.10e-164 488
Msa0828920 AT3G56960.2 42.521 595 310 11 9 591 68 642 1.84e-161 481
Msa0828920 AT3G56960.1 42.521 595 310 11 9 591 68 642 1.84e-161 481
Msa0828920 AT2G26420.1 42.687 588 280 11 6 591 46 578 5.93e-154 459
Msa0828920 AT2G26420.2 42.687 588 280 11 6 591 46 578 6.43e-154 459
Msa0828920 AT2G26420.3 42.687 588 280 11 6 591 46 578 6.43e-154 459
Msa0828920 AT1G77740.2 42.321 586 283 12 9 591 73 606 3.74e-144 435
Msa0828920 AT1G77740.1 42.321 586 283 12 9 591 73 606 3.74e-144 435
Msa0828920 AT1G21980.1 38.605 588 301 12 9 591 72 604 1.06e-126 390
Msa0828920 AT1G01460.1 41.850 227 131 1 365 591 3 228 3.38e-57 198
Msa0828920 AT4G01190.3 42.731 227 124 4 363 589 8 228 6.20e-53 186
Msa0828920 AT4G01190.2 42.731 227 124 4 363 589 8 228 6.20e-53 186
Msa0828920 AT4G01190.1 44.444 198 109 1 392 589 22 218 1.07e-52 186
Msa0828920 AT4G17080.1 34.270 178 117 0 10 187 254 431 2.99e-27 116
Msa0828920 AT4G17080.1 35.625 160 103 0 35 194 256 415 8.65e-22 99.8
Msa0828920 AT4G17080.1 32.836 134 90 0 10 143 300 433 1.99e-17 85.9
Msa0828920 AT4G17080.1 36.429 140 87 1 56 195 233 370 8.22e-15 77.8
Msa0828920 AT1G77660.1 35.429 175 113 0 12 186 177 351 4.30e-27 114
Msa0828920 AT1G77660.1 33.099 142 92 1 4 142 212 353 1.87e-15 79.3
Msa0828920 AT1G77660.1 35.088 114 74 0 82 195 178 291 5.43e-11 65.5
Msa0828920 AT2G35170.1 35.135 185 119 1 3 187 217 400 1.44e-26 114
Msa0828920 AT2G35170.1 34.028 144 86 2 56 195 201 339 1.74e-12 70.5
Msa0828920 AT2G35170.1 33.333 123 82 0 11 133 293 415 2.62e-12 69.7
Msa0828920 AT1G21920.1 34.091 176 116 0 12 187 173 348 6.78e-26 110
Msa0828920 AT1G21920.1 32.331 133 90 0 10 142 217 349 3.09e-14 75.5
Msa0828920 AT1G21920.1 35.965 114 73 0 82 195 174 287 1.80e-11 67.0
Msa0828920 AT1G21920.2 34.091 176 116 0 12 187 173 348 9.81e-26 109
Msa0828920 AT1G21920.2 32.331 133 90 0 10 142 217 349 4.33e-14 74.7
Msa0828920 AT1G21920.2 35.965 114 73 0 82 195 174 287 1.97e-11 66.6
Msa0828920 AT1G34260.2 30.189 212 125 9 392 588 1180 1383 6.76e-17 85.5
Msa0828920 AT1G34260.1 30.189 212 125 9 392 588 1180 1383 6.76e-17 85.5
Msa0828920 AT1G34260.3 30.189 212 125 9 392 588 1180 1383 6.76e-17 85.5
Msa0828920 AT1G34260.4 30.189 212 125 9 392 588 940 1143 9.47e-17 84.7
Msa0828920 AT4G33240.6 31.390 223 130 9 380 588 1444 1657 3.33e-15 80.1
Msa0828920 AT4G33240.1 31.390 223 130 9 380 588 1444 1657 3.33e-15 80.1
Msa0828920 AT4G33240.7 31.390 223 130 9 380 588 1444 1657 3.33e-15 80.1
Msa0828920 AT4G33240.2 31.390 223 130 9 380 588 1443 1656 3.42e-15 80.1
Msa0828920 AT4G33240.3 31.390 223 130 9 380 588 1440 1653 3.51e-15 80.1
Msa0828920 AT4G33240.5 31.390 223 130 9 380 588 1404 1617 3.58e-15 80.1
Msa0828920 AT4G33240.4 31.390 223 130 9 380 588 1441 1654 3.69e-15 80.1
Msa0828920 AT1G71010.1 33.333 162 94 5 440 588 1417 1577 1.13e-13 75.1
Msa0828920 AT1G75010.1 30.709 127 88 0 61 187 604 730 1.31e-11 68.2

Find 141 sgRNAs with CRISPR-Local

Find 277 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAATATCACTAATTTATT+TGG 0.100499 5_4:+1853859 None:intergenic
CAGATGTTTACGAAGGTTTA+TGG 0.157602 5_4:-1853370 Msa0828920:CDS
CTACGGTTAAATCTTGAATT+TGG 0.182290 5_4:+1853032 None:intergenic
CCTACATGATGGTAGTATTT+AGG 0.206353 5_4:+1851845 None:intergenic
AACTGCTTAATACTATTCTT+TGG 0.216939 5_4:-1854757 Msa0828920:five_prime_UTR
TTCTCTAGACTGCAAGTTCT+TGG 0.217156 5_4:-1850388 Msa0828920:CDS
TTTGATGGTTGTTGGTTAAA+TGG 0.249491 5_4:-1853224 Msa0828920:CDS
TTTGAGAGAAATGTTCAAAT+TGG 0.275332 5_4:-1852158 Msa0828920:CDS
GAAGTTCGGTCGTCTGATTT+TGG 0.281272 5_4:-1852427 Msa0828920:CDS
TCAGATGGAGGACTTTATAT+TGG 0.295938 5_4:-1853170 Msa0828920:CDS
GAAGTATCTATATTGGAAAT+TGG 0.296465 5_4:-1853301 Msa0828920:CDS
AACACTGAGAGGTGGGAAAA+AGG 0.313597 5_4:-1851774 Msa0828920:intron
ACTCTTATTACAAAATTCTT+CGG 0.317002 5_4:-1851809 Msa0828920:CDS
GTAAAGAAGAGTTCAATTAT+AGG 0.329256 5_4:-1852708 Msa0828920:CDS
TATGAAGGGGATTGGATAGA+TGG 0.337976 5_4:-1853569 Msa0828920:CDS
CTCTTATTACAAAATTCTTC+GGG 0.342573 5_4:-1851808 Msa0828920:CDS
ATCTCAAAGTACTTCATTGT+TGG 0.345418 5_4:-1852958 Msa0828920:CDS
ATTATTGCCCTATGGTCTTC+AGG 0.349462 5_4:-1852318 Msa0828920:intron
AATGATGGAAGTATCTATAT+TGG 0.350708 5_4:-1853308 Msa0828920:CDS
ACTGCTTAATACTATTCTTT+GGG 0.353424 5_4:-1854756 Msa0828920:five_prime_UTR
AAAGGAACATTCTATCCTTC+TGG 0.363846 5_4:-1853119 Msa0828920:CDS
CTCAGATTGACCTTCATTAG+AGG 0.364250 5_4:+1852863 None:intergenic
GTTTAACCTTTCAAAAGAAA+TGG 0.366422 5_4:-1853900 Msa0828920:five_prime_UTR
GGTATCTGGTATGAGTAATA+TGG 0.375485 5_4:-1852841 Msa0828920:CDS
AAGTACGAGGGCGAATTCTC+CGG 0.383441 5_4:-1853503 Msa0828920:CDS
ATCGCATAATAAGAACAAAA+AGG 0.391301 5_4:-1852754 Msa0828920:CDS
TAATTTATTTGGATAACTTC+AGG 0.393216 5_4:+1853870 None:intergenic
ATTCTCATTGCTATGAAGAT+TGG 0.396427 5_4:+1853064 None:intergenic
ATGTTTCAGATTGCTCTATC+AGG 0.403238 5_4:-1853983 Msa0828920:intron
ACCTTCGTAAACATCTGAAT+TGG 0.404965 5_4:+1853376 None:intergenic
CCCTCGTACTTTGCTCCTGA+TGG 0.405502 5_4:+1853515 None:intergenic
TTGGAGAAAGAGCTAGGATA+AGG 0.407652 5_4:-1852408 Msa0828920:CDS
GAAGGTCAATCTGAGGTATC+TGG 0.410812 5_4:-1852855 Msa0828920:CDS
CAGGGAAAGGGATACTTATA+TGG 0.412305 5_4:-1853538 Msa0828920:CDS
CGGTTCCTGCACGCGAAGTT+CGG 0.417255 5_4:-1852441 Msa0828920:CDS
TCTAAAATACGAATTTCATA+TGG 0.420253 5_4:-1851535 Msa0828920:CDS
AACAACCATCTCTAAAGAAA+TGG 0.425820 5_4:-1853091 Msa0828920:CDS
TTAATTTGCAGCTTGGCATC+AGG 0.428757 5_4:-1852650 Msa0828920:intron
GGGATTATGAAGTGGTTTAA+CGG 0.429532 5_4:-1853254 Msa0828920:CDS
TTAGGTGCGGTTTGTTGTCA+TGG 0.434098 5_4:-1851676 Msa0828920:intron
GATCGGTATATGAAGGGGAT+TGG 0.434561 5_4:-1853577 Msa0828920:CDS
TACGAAGGTTTATGGAAAGA+AGG 0.438135 5_4:-1853362 Msa0828920:CDS
GAAGAGAAGTCCTTTACAAA+TGG 0.438715 5_4:-1853668 Msa0828920:CDS
AATTGATTACAGTCTTCTGT+TGG 0.443015 5_4:-1850352 Msa0828920:CDS
GTATACTTCCCCACGGCAAC+GGG 0.447403 5_4:-1853619 Msa0828920:CDS
ACTGTTCTGTAGACTTCTAC+TGG 0.449316 5_4:-1852345 Msa0828920:CDS
GGAAAATTTACTTGGAATGA+TGG 0.451266 5_4:-1853323 Msa0828920:CDS
ATGAATGCTCATCACGGGAT+TGG 0.454271 5_4:-1853413 Msa0828920:CDS
CGACATATGCAATCGTCAGC+AGG 0.456001 5_4:+1852912 None:intergenic
CGAACTTCGCGTGCAGGAAC+CGG 0.457813 5_4:+1852442 None:intergenic
GATAATAGCTTCGGCGGTGT+TGG 0.458656 5_4:+1852679 None:intergenic
AAGGCAGCGGAAAATTTACT+TGG 0.465244 5_4:-1853331 Msa0828920:CDS
CTGAAGCTTAATTTGCAGCT+TGG 0.465574 5_4:-1852657 Msa0828920:CDS
ATTGATTACAGTCTTCTGTT+GGG 0.467533 5_4:-1850351 Msa0828920:CDS
GGGATACTTATATGGCCATC+AGG 0.475813 5_4:-1853530 Msa0828920:CDS
GAAAATAAGTATCCTGTGAC+AGG 0.476368 5_4:+1854683 None:intergenic
AGTACGAGGGCGAATTCTCC+GGG 0.479858 5_4:-1853502 Msa0828920:CDS
TAAGCTTCAGATAATAGCTT+CGG 0.480311 5_4:+1852670 None:intergenic
TAGAGCACAATCACCACAAA+TGG 0.481172 5_4:+1852114 None:intergenic
TGTCTACATTGGTAAATTCA+AGG 0.485501 5_4:-1853642 Msa0828920:CDS
TCATAGCAATGAGAATTGTA+AGG 0.491080 5_4:-1853057 Msa0828920:CDS
CTGGAAAGATTATTGCCCTA+TGG 0.492808 5_4:-1852326 Msa0828920:CDS
AATGGACTTAGACAAGGATC+TGG 0.498528 5_4:-1853206 Msa0828920:CDS
CTAAAGGACATATCTTCAGC+TGG 0.501585 5_4:-1852094 Msa0828920:CDS
CACCTCTCAGTGTTATTCGA+TGG 0.502502 5_4:+1851783 None:intergenic
TTTCATAACTTCCTACATGA+TGG 0.502637 5_4:+1851834 None:intergenic
TATATTGGAACATGGAGTAA+AGG 0.502815 5_4:-1853155 Msa0828920:CDS
TTGTGGTGATTGTGCTCTAA+AGG 0.503067 5_4:-1852110 Msa0828920:CDS
TAGGTGCGGTTTGTTGTCAT+GGG 0.512383 5_4:-1851675 Msa0828920:CDS
GGGAATTATCTTCACGGTCA+CGG 0.512896 5_4:-1853482 Msa0828920:CDS
TGTGTACTTCCCGTTGCCGT+GGG 0.513815 5_4:+1853610 None:intergenic
GAAGGAATTCGCGAAGGCAG+CGG 0.514143 5_4:-1853344 Msa0828920:CDS
GTACACCATTTCTTTAGAGA+TGG 0.515782 5_4:+1853086 None:intergenic
GGTCTGCGATAACGCGGATG+AGG 0.519666 5_4:+1852884 None:intergenic
GACGACCGAACTTCGCGTGC+AGG 0.520685 5_4:+1852436 None:intergenic
ATGTGTACTTCCCGTTGCCG+TGG 0.523495 5_4:+1853609 None:intergenic
GATAAGGATGTACTTTCCTA+AGG 0.524503 5_4:-1852392 Msa0828920:CDS
AAAGGCCTAAAGGACGGAAA+AGG 0.524731 5_4:-1853137 Msa0828920:CDS
GGTATACTTCCCCACGGCAA+CGG 0.526432 5_4:-1853620 Msa0828920:CDS
GTCAATTGTGAACCCTCCTT+AGG 0.529080 5_4:+1852376 None:intergenic
TTCTCCGGGAATTATCTTCA+CGG 0.530694 5_4:-1853488 Msa0828920:CDS
AATGAATACCTGAAGACCAT+AGG 0.538150 5_4:+1852310 None:intergenic
TCAGATGGATCGGTATATGA+AGG 0.538278 5_4:-1853584 Msa0828920:CDS
CCTAAATACTACCATCATGT+AGG 0.550012 5_4:-1851845 Msa0828920:CDS
GTCTACATTGGTAAATTCAA+GGG 0.551103 5_4:-1853641 Msa0828920:CDS
AAGGATGTACTTTCCTAAGG+AGG 0.552053 5_4:-1852389 Msa0828920:CDS
CCATCGTCGTTACGATCTGA+AGG 0.555421 5_4:-1851625 Msa0828920:CDS
TGGAGGATGAATGCTCATCA+CGG 0.558790 5_4:-1853419 Msa0828920:CDS
GTAGACATCACCATTTGTAA+AGG 0.559629 5_4:+1853658 None:intergenic
GTGACCGTGAAGATAATTCC+CGG 0.561940 5_4:+1853484 None:intergenic
ACAAATGGTGATGTCTACAT+TGG 0.565018 5_4:-1853653 Msa0828920:CDS
TAGTGATATTCTGCTAGTCA+TGG 0.565239 5_4:-1853849 Msa0828920:exon
ATGAATACCTGAAGACCATA+GGG 0.565904 5_4:+1852311 None:intergenic
GTACACATGGTCAGATGGAT+CGG 0.577176 5_4:-1853594 Msa0828920:CDS
GATGGTTGTTTGCTACCAGA+AGG 0.578528 5_4:+1853104 None:intergenic
GGAGGATGAATGCTCATCAC+GGG 0.578586 5_4:-1853418 Msa0828920:CDS
CAGATGGATCGGTATATGAA+GGG 0.578591 5_4:-1853583 Msa0828920:CDS
TTATCGCAGACCTCTAATGA+AGG 0.580718 5_4:-1852873 Msa0828920:CDS
AACATGGAGTAAAGGCCTAA+AGG 0.583252 5_4:-1853147 Msa0828920:CDS
AACGGCTGTGTTTACACTGG+TGG 0.584384 5_4:-1853443 Msa0828920:CDS
AAAAGGGAAAATAGATGGAA+GGG 0.584921 5_4:-1853276 Msa0828920:CDS
CCTTCAGATCGTAACGACGA+TGG 0.585986 5_4:+1851625 None:intergenic
TTCGAAAGGGAATATATGCA+AGG 0.587505 5_4:-1852816 Msa0828920:CDS
GATCTGAAGGGATCTTCTCA+AGG 0.588048 5_4:-1851612 Msa0828920:CDS
TCCAATTCAGATGTTTACGA+AGG 0.595150 5_4:-1853377 Msa0828920:CDS
AAAGGGAAAATAGATGGAAG+GGG 0.596052 5_4:-1853275 Msa0828920:CDS
AGGATGTACTTTCCTAAGGA+GGG 0.596234 5_4:-1852388 Msa0828920:CDS
GGGAAGTACACATGGTCAGA+TGG 0.596249 5_4:-1853599 Msa0828920:CDS
ATCGAATAACACTGAGAGGT+GGG 0.596556 5_4:-1851781 Msa0828920:CDS
GAGTACATGATGTCCATTTG+TGG 0.605960 5_4:-1852127 Msa0828920:CDS
ATTAGAGGTCTGCGATAACG+CGG 0.609836 5_4:+1852878 None:intergenic
TGGTTAAATGGACTTAGACA+AGG 0.611568 5_4:-1853212 Msa0828920:CDS
GACATATGCAATCGTCAGCA+GGG 0.613342 5_4:+1852913 None:intergenic
ACAAATGGACATCATGTACT+CGG 0.617112 5_4:+1852129 None:intergenic
CATCGAATAACACTGAGAGG+TGG 0.622054 5_4:-1851782 Msa0828920:CDS
CTGCTAGTCATGGAGGACAA+TGG 0.627968 5_4:-1853839 Msa0828920:exon
TCAATTCTCAGGTACACTGT+AGG 0.629207 5_4:-1852475 Msa0828920:intron
GCTTCAGATAATAGCTTCGG+CGG 0.629314 5_4:+1852673 None:intergenic
CTCTAATGAAGGTCAATCTG+AGG 0.630043 5_4:-1852862 Msa0828920:CDS
TAGTCATGGAGGACAATGGA+AGG 0.633428 5_4:-1853835 Msa0828920:intron
AAGGGAAAATAGATGGAAGG+GGG 0.634233 5_4:-1853274 Msa0828920:CDS
TGGAGTAAAGGCCTAAAGGA+CGG 0.638246 5_4:-1853143 Msa0828920:CDS
GTGTTTACACTGGTGGATGG+AGG 0.638524 5_4:-1853436 Msa0828920:CDS
TGGAAAGAAGGAATTCGCGA+AGG 0.641463 5_4:-1853350 Msa0828920:CDS
AGATGGATCGGTATATGAAG+GGG 0.644736 5_4:-1853582 Msa0828920:CDS
GAGGACTTTATATTGGAACA+TGG 0.648337 5_4:-1853163 Msa0828920:CDS
GCCATCAGGAGCAAAGTACG+AGG 0.650915 5_4:-1853516 Msa0828920:CDS
TATGCAAGGAGTTCTAATTG+TGG 0.655152 5_4:-1852802 Msa0828920:CDS
AGAAATGTTCAAATTGGACG+CGG 0.655936 5_4:-1852152 Msa0828920:CDS
GCTGTGTTTACACTGGTGGA+TGG 0.657960 5_4:-1853439 Msa0828920:CDS
TGATATTCTGCTAGTCATGG+AGG 0.663987 5_4:-1853846 Msa0828920:exon
TCGAACGGCTGTGTTTACAC+TGG 0.664113 5_4:-1853446 Msa0828920:CDS
TTCAAGGGTATACTTCCCCA+CGG 0.668280 5_4:-1853626 Msa0828920:CDS
ACGGCAACGGGAAGTACACA+TGG 0.669163 5_4:-1853607 Msa0828920:CDS
ATCCATCGAATAACACTGAG+AGG 0.670109 5_4:-1851785 Msa0828920:CDS
GGAACATTCACAAAATCGAA+CGG 0.670799 5_4:-1853461 Msa0828920:CDS
CATCGTCGTTACGATCTGAA+GGG 0.676822 5_4:-1851624 Msa0828920:CDS
GTGTACTTCCCGTTGCCGTG+GGG 0.689730 5_4:+1853611 None:intergenic
AAGTCTACAGAACAGTGTGG+AGG 0.692411 5_4:+1852352 None:intergenic
TAGAAGTCTACAGAACAGTG+TGG 0.705962 5_4:+1852349 None:intergenic
CCATCAGGAGCAAAGTACGA+GGG 0.707876 5_4:-1853515 Msa0828920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGATTATTGAAAATAA+TGG - chr5_4:1850925-1850944 Msa0828920:intron 10.0%
!! AACTTAATTAAAAGTTAATA+TGG + chr5_4:1853128-1853147 None:intergenic 10.0%
!!! AATATCTTTTGTTTTATTTA+AGG + chr5_4:1853893-1853912 None:intergenic 10.0%
!! AATAAAAATATTTGTTTCAT+TGG + chr5_4:1853923-1853942 None:intergenic 10.0%
!!! CTATATTTTATATGTAATAA+TGG - chr5_4:1853956-1853975 Msa0828920:five_prime_UTR 10.0%
!!! TCAATAATCTTTTTATTAGT+GGG + chr5_4:1850919-1850938 None:intergenic 15.0%
!!! TTCAATAATCTTTTTATTAG+TGG + chr5_4:1850920-1850939 None:intergenic 15.0%
!! CACTTGAATATTTATTTATT+TGG - chr5_4:1852552-1852571 Msa0828920:intron 15.0%
!!! ATATGATTTTATGATTATTG+TGG - chr5_4:1852837-1852856 Msa0828920:CDS 15.0%
!!! TATTGCTATTAATTTTATCT+TGG + chr5_4:1853517-1853536 None:intergenic 15.0%
!!! AGTAATACAATTTTACTTTT+TGG - chr5_4:1853792-1853811 Msa0828920:intron 15.0%
!! AATAAAACAAAAGATATTAG+TGG - chr5_4:1853895-1853914 Msa0828920:five_prime_UTR 15.0%
!!! AAAATCATCTGTTTAATTTT+CGG + chr5_4:1854345-1854364 None:intergenic 15.0%
!! ATATAAGTGTAAAGAAATAT+TGG - chr5_4:1854370-1854389 Msa0828920:intron 15.0%
!! TAAAAATCAACATATCTGTT+AGG - chr5_4:1850831-1850850 Msa0828920:intron 20.0%
!!! TAATTAGTTTCTTTATTCTC+TGG + chr5_4:1850890-1850909 None:intergenic 20.0%
!! AGATTATTGAAAATAATGGT+TGG - chr5_4:1850929-1850948 Msa0828920:intron 20.0%
!! TAATTTATTTGGATAACTTC+AGG + chr5_4:1851224-1851243 None:intergenic 20.0%
!! CAGAATATCACTAATTTATT+TGG + chr5_4:1851235-1851254 None:intergenic 20.0%
!! TATATTGGAAATTGGAAAAA+AGG - chr5_4:1851798-1851817 Msa0828920:CDS 20.0%
!! ATATTGGAAATTGGAAAAAA+GGG - chr5_4:1851799-1851818 Msa0828920:CDS 20.0%
!! TATCAGAATAACATGTATAT+AGG - chr5_4:1852803-1852822 Msa0828920:CDS 20.0%
!! ACTAAAAAAATATGAGCTAA+AGG - chr5_4:1853074-1853093 Msa0828920:CDS 20.0%
!! ACTCTTATTACAAAATTCTT+CGG - chr5_4:1853282-1853301 Msa0828920:CDS 20.0%
!!! AGAAAACAACAAAGTATTTA+TGG + chr5_4:1853392-1853411 None:intergenic 20.0%
!!! TCTAAAATACGAATTTCATA+TGG - chr5_4:1853556-1853575 Msa0828920:CDS 20.0%
!! AAAAGTTACTCTTATAAAGT+AGG + chr5_4:1853617-1853636 None:intergenic 20.0%
!! TACTAACATAATCATGAAAT+AGG + chr5_4:1853712-1853731 None:intergenic 20.0%
!!! ATACAATTTTACTTTTTGGA+CGG - chr5_4:1853796-1853815 Msa0828920:intron 20.0%
! AACTGCTTAATACTATTCTT+TGG - chr5_4:1850334-1850353 Msa0828920:CDS 25.0%
! ACTGCTTAATACTATTCTTT+GGG - chr5_4:1850335-1850354 Msa0828920:CDS 25.0%
! AAAAATTGAGATGAGAAAGA+TGG + chr5_4:1850448-1850467 None:intergenic 25.0%
! AAGGATTACAGAAAAGTTTA+GGG - chr5_4:1850780-1850799 Msa0828920:intron 25.0%
! GGTAAAAACATCTAAATAAG+TGG - chr5_4:1850950-1850969 Msa0828920:intron 25.0%
! AAAACATCTAAATAAGTGGA+TGG - chr5_4:1850954-1850973 Msa0828920:intron 25.0%
! AAAATTCCAAAGACAGAAAA+AGG + chr5_4:1850985-1851004 None:intergenic 25.0%
!! TGTTTTCAGACTCATTATAT+TGG - chr5_4:1851079-1851098 Msa0828920:intron 25.0%
! GTTTAACCTTTCAAAAGAAA+TGG - chr5_4:1851191-1851210 Msa0828920:intron 25.0%
! CAAAACAAAAGAGAATGAAT+AGG + chr5_4:1851309-1851328 None:intergenic 25.0%
!! TAACTGTTTCTTGTTAATGT+AGG - chr5_4:1851397-1851416 Msa0828920:intron 25.0%
!!! AATGATGGAAGTATCTATAT+TGG - chr5_4:1851783-1851802 Msa0828920:CDS 25.0%
!! GAAGTATCTATATTGGAAAT+TGG - chr5_4:1851790-1851809 Msa0828920:CDS 25.0%
! ATCGCATAATAAGAACAAAA+AGG - chr5_4:1852337-1852356 Msa0828920:CDS 25.0%
! GTAAAGAAGAGTTCAATTAT+AGG - chr5_4:1852383-1852402 Msa0828920:CDS 25.0%
!!! ATTATTTTTGCTCAATTCTC+AGG - chr5_4:1852605-1852624 Msa0828920:intron 25.0%
! ATGTGATTTCATATATTTCC+AGG - chr5_4:1852908-1852927 Msa0828920:CDS 25.0%
! TTTGAGAGAAATGTTCAAAT+TGG - chr5_4:1852933-1852952 Msa0828920:CDS 25.0%
! AATTAAGTACTTAAGAAGTC+AGG + chr5_4:1853182-1853201 None:intergenic 25.0%
! CTCTTATTACAAAATTCTTC+GGG - chr5_4:1853283-1853302 Msa0828920:CDS 25.0%
! ACTTCTTTCTTCTTGTTAAT+TGG - chr5_4:1853361-1853380 Msa0828920:CDS 25.0%
!!! GTTAGTATTTTATTTCCCAT+AGG - chr5_4:1853725-1853744 Msa0828920:intron 25.0%
!! ACTATCAAAATCTGAAATTG+TGG + chr5_4:1854514-1854533 None:intergenic 25.0%
!!! TTTCAGATTTTGATAGTACT+TGG - chr5_4:1854517-1854536 Msa0828920:intron 25.0%
TGACAGGAATAAAAACTGAA+AGG + chr5_4:1850395-1850414 None:intergenic 30.0%
!! AGGATACTTATTTTCATCCA+AGG - chr5_4:1850416-1850435 Msa0828920:intron 30.0%
!!! CTTTTGTTTTCTTTCTGTTC+TGG - chr5_4:1850470-1850489 Msa0828920:intron 30.0%
TGTATAGAAACATCAGAACT+AGG + chr5_4:1850527-1850546 None:intergenic 30.0%
AAAGTCAGATCAAAGAAAGA+TGG + chr5_4:1850556-1850575 None:intergenic 30.0%
TGAAACAATACATGCATGTT+GGG + chr5_4:1850674-1850693 None:intergenic 30.0%
GAGTGTAATCAACATCATAA+AGG + chr5_4:1850753-1850772 None:intergenic 30.0%
! ACTTTTCTGTAATCCTTGTT+AGG + chr5_4:1850777-1850796 None:intergenic 30.0%
CAAGGATTACAGAAAAGTTT+AGG - chr5_4:1850779-1850798 Msa0828920:intron 30.0%
!!! TTTCTGTCTTTGGAATTTTG+TGG - chr5_4:1850986-1851005 Msa0828920:intron 30.0%
!!! TTCTGTCTTTGGAATTTTGT+GGG - chr5_4:1850987-1851006 Msa0828920:intron 30.0%
! TCAACACTAAAGAATGCTTT+TGG + chr5_4:1851021-1851040 None:intergenic 30.0%
! TTAGTGTTGAAACCTGTTAA+AGG - chr5_4:1851031-1851050 Msa0828920:intron 30.0%
ACAACAATGAATCCTTTAAC+AGG + chr5_4:1851046-1851065 None:intergenic 30.0%
!!! AATGTGCCATTTCTTTTGAA+AGG + chr5_4:1851200-1851219 None:intergenic 30.0%
TGTCTACATTGGTAAATTCA+AGG - chr5_4:1851449-1851468 Msa0828920:intron 30.0%
GTCTACATTGGTAAATTCAA+GGG - chr5_4:1851450-1851469 Msa0828920:intron 30.0%
GGAAAATTTACTTGGAATGA+TGG - chr5_4:1851768-1851787 Msa0828920:intron 30.0%
TGGAAAAAAGGGAAAATAGA+TGG - chr5_4:1851810-1851829 Msa0828920:CDS 30.0%
AAAAAGGGAAAATAGATGGA+AGG - chr5_4:1851814-1851833 Msa0828920:CDS 30.0%
AAAAGGGAAAATAGATGGAA+GGG - chr5_4:1851815-1851834 Msa0828920:CDS 30.0%
!! TTTAACGGTGATGTTTTTGA+TGG - chr5_4:1851852-1851871 Msa0828920:CDS 30.0%
! TTTGATGGTTGTTGGTTAAA+TGG - chr5_4:1851867-1851886 Msa0828920:intron 30.0%
! GGAGTTTATAGATTTTCAGA+TGG - chr5_4:1851906-1851925 Msa0828920:intron 30.0%
! GTTTATAGATTTTCAGATGG+AGG - chr5_4:1851909-1851928 Msa0828920:intron 30.0%
TATATTGGAACATGGAGTAA+AGG - chr5_4:1851936-1851955 Msa0828920:intron 30.0%
! AACAACCATCTCTAAAGAAA+TGG - chr5_4:1852000-1852019 Msa0828920:intron 30.0%
ATTCTCATTGCTATGAAGAT+TGG + chr5_4:1852030-1852049 None:intergenic 30.0%
TCATAGCAATGAGAATTGTA+AGG - chr5_4:1852034-1852053 Msa0828920:CDS 30.0%
CTACGGTTAAATCTTGAATT+TGG + chr5_4:1852062-1852081 None:intergenic 30.0%
! ATCTCAAAGTACTTCATTGT+TGG - chr5_4:1852133-1852152 Msa0828920:CDS 30.0%
! TGAGTAATATGGTTTTCGAA+AGG - chr5_4:1852261-1852280 Msa0828920:intron 30.0%
! GAGTAATATGGTTTTCGAAA+GGG - chr5_4:1852262-1852281 Msa0828920:intron 30.0%
TAAGCTTCAGATAATAGCTT+CGG + chr5_4:1852424-1852443 None:intergenic 30.0%
TTTCATAACTTCCTACATGA+TGG + chr5_4:1853260-1853279 None:intergenic 30.0%
AAGAAGTAAAGACGGATATT+AGG + chr5_4:1853348-1853367 None:intergenic 30.0%
CAAGAAGAAAGAAGTAAAGA+CGG + chr5_4:1853356-1853375 None:intergenic 30.0%
!!! ACTTTGTTGTTTTCTTCGTT+AGG - chr5_4:1853397-1853416 Msa0828920:CDS 30.0%
! AGTAGTAAAGCATTTCCTAT+GGG + chr5_4:1853743-1853762 None:intergenic 30.0%
! AAGTAGTAAAGCATTTCCTA+TGG + chr5_4:1853744-1853763 None:intergenic 30.0%
! CTTTGTTATCGTAAGTACTA+TGG - chr5_4:1853761-1853780 Msa0828920:intron 30.0%
CTAATAATACTGGCACAATA+TGG - chr5_4:1853820-1853839 Msa0828920:intron 30.0%
ATTTGTGAGGGATATCAATA+AGG + chr5_4:1854059-1854078 None:intergenic 30.0%
TCTCTCTATTTGTAAAGCTT+TGG + chr5_4:1854210-1854229 None:intergenic 30.0%
ATCTCTTATTGACAGTATCA+TGG - chr5_4:1854294-1854313 Msa0828920:intron 30.0%
!!! ATGACCTGACTTTATTTTTC+TGG - chr5_4:1854669-1854688 Msa0828920:intron 30.0%
TGAACCAGAAAAATAAAGTC+AGG + chr5_4:1854676-1854695 None:intergenic 30.0%
!!! TGACTTTATTTTTCTGGTTC+AGG - chr5_4:1854675-1854694 Msa0828920:intron 30.0%
AATTGATTACAGTCTTCTGT+TGG - chr5_4:1854739-1854758 Msa0828920:five_prime_UTR 30.0%
ATTGATTACAGTCTTCTGTT+GGG - chr5_4:1854740-1854759 Msa0828920:five_prime_UTR 30.0%
! TCAGTTTTTATTCCTGTCAC+AGG - chr5_4:1850396-1850415 Msa0828920:CDS 35.0%
GAAAATAAGTATCCTGTGAC+AGG + chr5_4:1850411-1850430 None:intergenic 35.0%
CTGAAACAATACATGCATGT+TGG + chr5_4:1850675-1850694 None:intergenic 35.0%
CAACATGCATGTATTGTTTC+AGG - chr5_4:1850673-1850692 Msa0828920:intron 35.0%
TTGATTACACTCTCCTAACA+AGG - chr5_4:1850761-1850780 Msa0828920:intron 35.0%
TTCCAAAGACAGAAAAAGGA+GGG + chr5_4:1850981-1851000 None:intergenic 35.0%
ATTCCAAAGACAGAAAAAGG+AGG + chr5_4:1850982-1851001 None:intergenic 35.0%
ATGTTTCAGATTGCTCTATC+AGG - chr5_4:1851108-1851127 Msa0828920:intron 35.0%
ATCAGGTCACAATAAAAAGC+TGG - chr5_4:1851125-1851144 Msa0828920:intron 35.0%
TAGTGATATTCTGCTAGTCA+TGG - chr5_4:1851242-1851261 Msa0828920:intron 35.0%
GAAGAGAAGTCCTTTACAAA+TGG - chr5_4:1851423-1851442 Msa0828920:intron 35.0%
GTAGACATCACCATTTGTAA+AGG + chr5_4:1851436-1851455 None:intergenic 35.0%
! ACAAATGGTGATGTCTACAT+TGG - chr5_4:1851438-1851457 Msa0828920:intron 35.0%
TGGATAGATGGAAAAATGAC+AGG - chr5_4:1851534-1851553 Msa0828920:CDS 35.0%
GGATAGATGGAAAAATGACA+GGG - chr5_4:1851535-1851554 Msa0828920:CDS 35.0%
ATGGAAAAATGACAGGGAAA+GGG - chr5_4:1851541-1851560 Msa0828920:CDS 35.0%
GGAACATTCACAAAATCGAA+CGG - chr5_4:1851630-1851649 Msa0828920:CDS 35.0%
TCCAATTCAGATGTTTACGA+AGG - chr5_4:1851714-1851733 Msa0828920:intron 35.0%
ACCTTCGTAAACATCTGAAT+TGG + chr5_4:1851718-1851737 None:intergenic 35.0%
CAGATGTTTACGAAGGTTTA+TGG - chr5_4:1851721-1851740 Msa0828920:intron 35.0%
TACGAAGGTTTATGGAAAGA+AGG - chr5_4:1851729-1851748 Msa0828920:intron 35.0%
AAAGGGAAAATAGATGGAAG+GGG - chr5_4:1851816-1851835 Msa0828920:CDS 35.0%
GGGATTATGAAGTGGTTTAA+CGG - chr5_4:1851837-1851856 Msa0828920:CDS 35.0%
!! GTGATGTTTTTGATGGTTGT+TGG - chr5_4:1851859-1851878 Msa0828920:CDS 35.0%
! TGGTTAAATGGACTTAGACA+AGG - chr5_4:1851879-1851898 Msa0828920:intron 35.0%
TCAGATGGAGGACTTTATAT+TGG - chr5_4:1851921-1851940 Msa0828920:intron 35.0%
GAGGACTTTATATTGGAACA+TGG - chr5_4:1851928-1851947 Msa0828920:intron 35.0%
AAAGGAACATTCTATCCTTC+TGG - chr5_4:1851972-1851991 Msa0828920:intron 35.0%
! GTACACCATTTCTTTAGAGA+TGG + chr5_4:1852008-1852027 None:intergenic 35.0%
! TGAACTTTTTGATCTACCAC+TGG + chr5_4:1852105-1852124 None:intergenic 35.0%
GGTATCTGGTATGAGTAATA+TGG - chr5_4:1852250-1852269 Msa0828920:intron 35.0%
TTCGAAAGGGAATATATGCA+AGG - chr5_4:1852275-1852294 Msa0828920:intron 35.0%
! TATGCAAGGAGTTCTAATTG+TGG - chr5_4:1852289-1852308 Msa0828920:intron 35.0%
CACTGTAGGAAAAATTACAC+CGG - chr5_4:1852630-1852649 Msa0828920:intron 35.0%
GATAAGGATGTACTTTCCTA+AGG - chr5_4:1852699-1852718 Msa0828920:CDS 35.0%
ATGAATACCTGAAGACCATA+GGG + chr5_4:1852783-1852802 None:intergenic 35.0%
AATGAATACCTGAAGACCAT+AGG + chr5_4:1852784-1852803 None:intergenic 35.0%
GAACATTTCTCTCAAATTCC+TGG + chr5_4:1852929-1852948 None:intergenic 35.0%
AGAAATGTTCAAATTGGACG+CGG - chr5_4:1852939-1852958 Msa0828920:CDS 35.0%
ACAAATGGACATCATGTACT+CGG + chr5_4:1852965-1852984 None:intergenic 35.0%
ATATCTTCAGCTGGAAAAAG+TGG - chr5_4:1853006-1853025 Msa0828920:CDS 35.0%
! GCATACATTCCTCTTGTTAT+AGG - chr5_4:1853206-1853225 Msa0828920:CDS 35.0%
CCTACATGATGGTAGTATTT+AGG + chr5_4:1853249-1853268 None:intergenic 35.0%
CCTAAATACTACCATCATGT+AGG - chr5_4:1853246-1853265 Msa0828920:CDS 35.0%
! TGTAGCAATTTTGCTCTTCT+AGG - chr5_4:1853672-1853691 Msa0828920:CDS 35.0%
!! TTTGGACGGACTAATAATAC+TGG - chr5_4:1853810-1853829 Msa0828920:intron 35.0%
ATATCCCTCACAAATTGATG+CGG - chr5_4:1854064-1854083 Msa0828920:intron 35.0%
!!! GCTTGTGGATATTTTAACCA+AGG + chr5_4:1854146-1854165 None:intergenic 35.0%
TGGTTAAAATATCCACAAGC+CGG - chr5_4:1854146-1854165 Msa0828920:intron 35.0%
GACAGTATCATGGTAGTAAT+AGG - chr5_4:1854304-1854323 Msa0828920:intron 35.0%
TCGCTAAAATTTGCAATAGC+AGG + chr5_4:1854431-1854450 None:intergenic 35.0%
AATTACTTTCCCTTCAGACA+AGG + chr5_4:1854632-1854651 None:intergenic 35.0%
TGAAGGGAAAGTAATTTACC+TGG - chr5_4:1854636-1854655 Msa0828920:intron 35.0%
AGGTCACAATAAAAAGCTGG+TGG - chr5_4:1851128-1851147 Msa0828920:intron 40.0%
TGATATTCTGCTAGTCATGG+AGG - chr5_4:1851245-1851264 Msa0828920:intron 40.0%
TCAGATGGATCGGTATATGA+AGG - chr5_4:1851507-1851526 Msa0828920:CDS 40.0%
CAGATGGATCGGTATATGAA+GGG - chr5_4:1851508-1851527 Msa0828920:CDS 40.0%
AGATGGATCGGTATATGAAG+GGG - chr5_4:1851509-1851528 Msa0828920:CDS 40.0%
TATGAAGGGGATTGGATAGA+TGG - chr5_4:1851522-1851541 Msa0828920:CDS 40.0%
GATGGAAAAATGACAGGGAA+AGG - chr5_4:1851540-1851559 Msa0828920:CDS 40.0%
CAGGGAAAGGGATACTTATA+TGG - chr5_4:1851553-1851572 Msa0828920:CDS 40.0%
TTCTCCGGGAATTATCTTCA+CGG - chr5_4:1851603-1851622 Msa0828920:CDS 40.0%
AAGGCAGCGGAAAATTTACT+TGG - chr5_4:1851760-1851779 Msa0828920:intron 40.0%
AAGGGAAAATAGATGGAAGG+GGG - chr5_4:1851817-1851836 Msa0828920:CDS 40.0%
! AATGGACTTAGACAAGGATC+TGG - chr5_4:1851885-1851904 Msa0828920:intron 40.0%
AACATGGAGTAAAGGCCTAA+AGG - chr5_4:1851944-1851963 Msa0828920:intron 40.0%
! ATGTTCCTTTTCCGTCCTTT+AGG + chr5_4:1851962-1851981 None:intergenic 40.0%
!!! GAGCTTTTTCAGAGAGACTA+CGG + chr5_4:1852079-1852098 None:intergenic 40.0%
TTATCGCAGACCTCTAATGA+AGG - chr5_4:1852218-1852237 Msa0828920:intron 40.0%
CTCAGATTGACCTTCATTAG+AGG + chr5_4:1852231-1852250 None:intergenic 40.0%
CTCTAATGAAGGTCAATCTG+AGG - chr5_4:1852229-1852248 Msa0828920:intron 40.0%
!! CTGAAGCTTAATTTGCAGCT+TGG - chr5_4:1852434-1852453 Msa0828920:CDS 40.0%
!! TTAATTTGCAGCTTGGCATC+AGG - chr5_4:1852441-1852460 Msa0828920:CDS 40.0%
TCAATTCTCAGGTACACTGT+AGG - chr5_4:1852616-1852635 Msa0828920:intron 40.0%
!! CTGATTTTGGAGAAAGAGCT+AGG - chr5_4:1852677-1852696 Msa0828920:CDS 40.0%
TTGGAGAAAGAGCTAGGATA+AGG - chr5_4:1852683-1852702 Msa0828920:CDS 40.0%
AAGGATGTACTTTCCTAAGG+AGG - chr5_4:1852702-1852721 Msa0828920:CDS 40.0%
AGGATGTACTTTCCTAAGGA+GGG - chr5_4:1852703-1852722 Msa0828920:CDS 40.0%
TAGAAGTCTACAGAACAGTG+TGG + chr5_4:1852745-1852764 None:intergenic 40.0%
ACTGTTCTGTAGACTTCTAC+TGG - chr5_4:1852746-1852765 Msa0828920:CDS 40.0%
CTGGAAAGATTATTGCCCTA+TGG - chr5_4:1852765-1852784 Msa0828920:CDS 40.0%
ATTATTGCCCTATGGTCTTC+AGG - chr5_4:1852773-1852792 Msa0828920:CDS 40.0%
GAGTACATGATGTCCATTTG+TGG - chr5_4:1852964-1852983 Msa0828920:CDS 40.0%
TAGAGCACAATCACCACAAA+TGG + chr5_4:1852980-1852999 None:intergenic 40.0%
TTGTGGTGATTGTGCTCTAA+AGG - chr5_4:1852981-1853000 Msa0828920:CDS 40.0%
CTAAAGGACATATCTTCAGC+TGG - chr5_4:1852997-1853016 Msa0828920:CDS 40.0%
! TAGTCACCTCTTTTGCTTGA+TGG - chr5_4:1853101-1853120 Msa0828920:CDS 40.0%
TATGGTCCATCAAGCAAAAG+AGG + chr5_4:1853110-1853129 None:intergenic 40.0%
TGAGCAGAACCTATAACAAG+AGG + chr5_4:1853218-1853237 None:intergenic 40.0%
ATCCATCGAATAACACTGAG+AGG - chr5_4:1853306-1853325 Msa0828920:CDS 40.0%
ATCGAATAACACTGAGAGGT+GGG - chr5_4:1853310-1853329 Msa0828920:CDS 40.0%
!! GTTGTTTTCTTCGTTAGGTG+CGG - chr5_4:1853402-1853421 Msa0828920:CDS 40.0%
TTCAGCTTTCTTGCTTTCGT+TGG - chr5_4:1853856-1853875 Msa0828920:five_prime_UTR 40.0%
AGGTATTTCGATTCATCGCT+GGG + chr5_4:1854039-1854058 None:intergenic 40.0%
! AAGGTATTTCGATTCATCGC+TGG + chr5_4:1854040-1854059 None:intergenic 40.0%
!! CTGATGAGAAATTGATAGCG+AGG - chr5_4:1854109-1854128 Msa0828920:intron 40.0%
GGTTAAAATATCCACAAGCC+GGG - chr5_4:1854147-1854166 Msa0828920:intron 40.0%
CTTCGTGCACTCATGAAAAA+AGG - chr5_4:1854247-1854266 Msa0828920:intron 40.0%
TGCAATAGCAGGATCTAACA+AGG + chr5_4:1854420-1854439 None:intergenic 40.0%
TAGAACTTCTCCTTGTCTGA+AGG - chr5_4:1854619-1854638 Msa0828920:intron 40.0%
AGAACTTCTCCTTGTCTGAA+GGG - chr5_4:1854620-1854639 Msa0828920:intron 40.0%
AGGGAAAGTAATTTACCTGG+TGG - chr5_4:1854639-1854658 Msa0828920:intron 40.0%
TTCTCTAGACTGCAAGTTCT+TGG - chr5_4:1854703-1854722 Msa0828920:five_prime_UTR 40.0%
GAGAAAGATGGAAGTGACCT+TGG + chr5_4:1850436-1850455 None:intergenic 45.0%
! GTCCCTCCTTTTTCTGTCTT+TGG - chr5_4:1850976-1850995 Msa0828920:intron 45.0%
TAGTCATGGAGGACAATGGA+AGG - chr5_4:1851256-1851275 Msa0828920:intron 45.0%
! TTCAAGGGTATACTTCCCCA+CGG - chr5_4:1851465-1851484 Msa0828920:intron 45.0%
GTACACATGGTCAGATGGAT+CGG - chr5_4:1851497-1851516 Msa0828920:intron 45.0%
GATCGGTATATGAAGGGGAT+TGG - chr5_4:1851514-1851533 Msa0828920:CDS 45.0%
GGGATACTTATATGGCCATC+AGG - chr5_4:1851561-1851580 Msa0828920:CDS 45.0%
GTGACCGTGAAGATAATTCC+CGG + chr5_4:1851610-1851629 None:intergenic 45.0%
GGGAATTATCTTCACGGTCA+CGG - chr5_4:1851609-1851628 Msa0828920:CDS 45.0%
TGGAGGATGAATGCTCATCA+CGG - chr5_4:1851672-1851691 Msa0828920:CDS 45.0%
ATGAATGCTCATCACGGGAT+TGG - chr5_4:1851678-1851697 Msa0828920:intron 45.0%
TGGAAAGAAGGAATTCGCGA+AGG - chr5_4:1851741-1851760 Msa0828920:intron 45.0%
ATGGAAGGGGGATTATGAAG+TGG - chr5_4:1851829-1851848 Msa0828920:CDS 45.0%
TGGAGTAAAGGCCTAAAGGA+CGG - chr5_4:1851948-1851967 Msa0828920:intron 45.0%
AAAGGCCTAAAGGACGGAAA+AGG - chr5_4:1851954-1851973 Msa0828920:intron 45.0%
! GATGGTTGTTTGCTACCAGA+AGG + chr5_4:1851990-1852009 None:intergenic 45.0%
GACATATGCAATCGTCAGCA+GGG + chr5_4:1852181-1852200 None:intergenic 45.0%
ATTAGAGGTCTGCGATAACG+CGG + chr5_4:1852216-1852235 None:intergenic 45.0%
! GAAGGTCAATCTGAGGTATC+TGG - chr5_4:1852236-1852255 Msa0828920:intron 45.0%
GCTTCAGATAATAGCTTCGG+CGG + chr5_4:1852421-1852440 None:intergenic 45.0%
! GAAGTTCGGTCGTCTGATTT+TGG - chr5_4:1852664-1852683 Msa0828920:CDS 45.0%
GTCAATTGTGAACCCTCCTT+AGG + chr5_4:1852718-1852737 None:intergenic 45.0%
AAGTCTACAGAACAGTGTGG+AGG + chr5_4:1852742-1852761 None:intergenic 45.0%
! CACCTCTCAGTGTTATTCGA+TGG + chr5_4:1853311-1853330 None:intergenic 45.0%
CATCGAATAACACTGAGAGG+TGG - chr5_4:1853309-1853328 Msa0828920:CDS 45.0%
AACACTGAGAGGTGGGAAAA+AGG - chr5_4:1853317-1853336 Msa0828920:CDS 45.0%
!! TTAGGTGCGGTTTGTTGTCA+TGG - chr5_4:1853415-1853434 Msa0828920:CDS 45.0%
!! TAGGTGCGGTTTGTTGTCAT+GGG - chr5_4:1853416-1853435 Msa0828920:CDS 45.0%
CATCGTCGTTACGATCTGAA+GGG - chr5_4:1853467-1853486 Msa0828920:CDS 45.0%
GATCTGAAGGGATCTTCTCA+AGG - chr5_4:1853479-1853498 Msa0828920:CDS 45.0%
GCTTTCTTGCTTTCGTTGGT+AGG - chr5_4:1853860-1853879 Msa0828920:five_prime_UTR 45.0%
!! AGAAATTGATAGCGAGGTCG+AGG - chr5_4:1854115-1854134 Msa0828920:intron 45.0%
TGAGTGCACGAAGCATATTG+AGG + chr5_4:1854240-1854259 None:intergenic 45.0%
CAGGTCATATCATAACCACC+AGG + chr5_4:1854657-1854676 None:intergenic 45.0%
!! AATAAAATAAAATACAATAA+TGG - chr5_4:1853995-1854014 Msa0828920:intron 5.0%
CTGCTAGTCATGGAGGACAA+TGG - chr5_4:1851252-1851271 Msa0828920:intron 50.0%
! GGGAAGTACACATGGTCAGA+TGG - chr5_4:1851492-1851511 Msa0828920:intron 50.0%
CCATCAGGAGCAAAGTACGA+GGG - chr5_4:1851576-1851595 Msa0828920:CDS 50.0%
AAGTACGAGGGCGAATTCTC+CGG - chr5_4:1851588-1851607 Msa0828920:CDS 50.0%
TCGAACGGCTGTGTTTACAC+TGG - chr5_4:1851645-1851664 Msa0828920:CDS 50.0%
AACGGCTGTGTTTACACTGG+TGG - chr5_4:1851648-1851667 Msa0828920:CDS 50.0%
GCTGTGTTTACACTGGTGGA+TGG - chr5_4:1851652-1851671 Msa0828920:CDS 50.0%
! GTGTTTACACTGGTGGATGG+AGG - chr5_4:1851655-1851674 Msa0828920:CDS 50.0%
GGAGGATGAATGCTCATCAC+GGG - chr5_4:1851673-1851692 Msa0828920:CDS 50.0%
TCTGAAAAAGCTCGAGCCAG+TGG - chr5_4:1852086-1852105 Msa0828920:CDS 50.0%
CGACATATGCAATCGTCAGC+AGG + chr5_4:1852182-1852201 None:intergenic 50.0%
GATAATAGCTTCGGCGGTGT+TGG + chr5_4:1852415-1852434 None:intergenic 50.0%
CCTTCAGATCGTAACGACGA+TGG + chr5_4:1853469-1853488 None:intergenic 50.0%
CCATCGTCGTTACGATCTGA+AGG - chr5_4:1853466-1853485 Msa0828920:CDS 50.0%
CCAGCCGCATCAATTTGTGA+GGG + chr5_4:1854071-1854090 None:intergenic 50.0%
ACCAGCCGCATCAATTTGTG+AGG + chr5_4:1854072-1854091 None:intergenic 50.0%
GGTATACTTCCCCACGGCAA+CGG - chr5_4:1851471-1851490 Msa0828920:intron 55.0%
GTATACTTCCCCACGGCAAC+GGG - chr5_4:1851472-1851491 Msa0828920:intron 55.0%
TGTGTACTTCCCGTTGCCGT+GGG + chr5_4:1851484-1851503 None:intergenic 55.0%
ATGTGTACTTCCCGTTGCCG+TGG + chr5_4:1851485-1851504 None:intergenic 55.0%
! ACGGCAACGGGAAGTACACA+TGG - chr5_4:1851484-1851503 Msa0828920:intron 55.0%
GCCATCAGGAGCAAAGTACG+AGG - chr5_4:1851575-1851594 Msa0828920:CDS 55.0%
CCCTCGTACTTTGCTCCTGA+TGG + chr5_4:1851579-1851598 None:intergenic 55.0%
AGTACGAGGGCGAATTCTCC+GGG - chr5_4:1851589-1851608 Msa0828920:CDS 55.0%
GAAGGAATTCGCGAAGGCAG+CGG - chr5_4:1851747-1851766 Msa0828920:intron 55.0%
CCCTCACAAATTGATGCGGC+TGG - chr5_4:1854068-1854087 Msa0828920:intron 55.0%
TCCTCATTCACCTCGCAACC+CGG + chr5_4:1854168-1854187 None:intergenic 55.0%
GTGTACTTCCCGTTGCCGTG+GGG + chr5_4:1851483-1851502 None:intergenic 60.0%
GGTCTGCGATAACGCGGATG+AGG + chr5_4:1852210-1852229 None:intergenic 60.0%
CGAACTTCGCGTGCAGGAAC+CGG + chr5_4:1852652-1852671 None:intergenic 60.0%
CGGTTCCTGCACGCGAAGTT+CGG - chr5_4:1852650-1852669 Msa0828920:intron 60.0%
GCGAGGTCGAGGTACAACCT+TGG - chr5_4:1854126-1854145 Msa0828920:intron 60.0%
TATCCACAAGCCGGGTTGCG+AGG - chr5_4:1854155-1854174 Msa0828920:intron 60.0%
GACGACCGAACTTCGCGTGC+AGG + chr5_4:1852658-1852677 None:intergenic 65.0%
! TCACCTCGCAACCCGGCTTG+TGG + chr5_4:1854161-1854180 None:intergenic 65.0%
GCCGGGTTGCGAGGTGAATG+AGG - chr5_4:1854164-1854183 Msa0828920:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 1850332 1854781 1850332 ID=Msa0828920;Name=Msa0828920
chr5_4 mRNA 1850332 1854781 1850332 ID=Msa0828920-mRNA-1;Parent=Msa0828920;Name=Msa0828920-mRNA-1;_AED=0.08;_eAED=0.08;_QI=250|1|1|1|0.71|0.62|8|0|593
chr5_4 exon 1850332 1850416 1850332 ID=Msa0828920-mRNA-1:exon:511;Parent=Msa0828920-mRNA-1
chr5_4 exon 1851507 1851694 1851507 ID=Msa0828920-mRNA-1:exon:510;Parent=Msa0828920-mRNA-1
chr5_4 exon 1851775 1851885 1851775 ID=Msa0828920-mRNA-1:exon:509;Parent=Msa0828920-mRNA-1
chr5_4 exon 1852018 1852183 1852018 ID=Msa0828920-mRNA-1:exon:508;Parent=Msa0828920-mRNA-1
chr5_4 exon 1852319 1852486 1852319 ID=Msa0828920-mRNA-1:exon:507;Parent=Msa0828920-mRNA-1
chr5_4 exon 1852651 1853694 1852651 ID=Msa0828920-mRNA-1:exon:506;Parent=Msa0828920-mRNA-1
chr5_4 exon 1853836 1853996 1853836 ID=Msa0828920-mRNA-1:exon:505;Parent=Msa0828920-mRNA-1
chr5_4 exon 1854676 1854781 1854676 ID=Msa0828920-mRNA-1:exon:504;Parent=Msa0828920-mRNA-1
chr5_4 five_prime_UTR 1854676 1854781 1854676 ID=Msa0828920-mRNA-1:five_prime_utr;Parent=Msa0828920-mRNA-1
chr5_4 five_prime_UTR 1853853 1853996 1853853 ID=Msa0828920-mRNA-1:five_prime_utr;Parent=Msa0828920-mRNA-1
chr5_4 CDS 1853836 1853852 1853836 ID=Msa0828920-mRNA-1:cds;Parent=Msa0828920-mRNA-1
chr5_4 CDS 1852651 1853694 1852651 ID=Msa0828920-mRNA-1:cds;Parent=Msa0828920-mRNA-1
chr5_4 CDS 1852319 1852486 1852319 ID=Msa0828920-mRNA-1:cds;Parent=Msa0828920-mRNA-1
chr5_4 CDS 1852018 1852183 1852018 ID=Msa0828920-mRNA-1:cds;Parent=Msa0828920-mRNA-1
chr5_4 CDS 1851775 1851885 1851775 ID=Msa0828920-mRNA-1:cds;Parent=Msa0828920-mRNA-1
chr5_4 CDS 1851507 1851694 1851507 ID=Msa0828920-mRNA-1:cds;Parent=Msa0828920-mRNA-1
chr5_4 CDS 1850332 1850416 1850332 ID=Msa0828920-mRNA-1:cds;Parent=Msa0828920-mRNA-1
Gene Sequence

>Msa0828920

ATGGAGGACAATGGAAGGTTTGAAGAGAAGTCCTTTACAAATGGTGATGTCTACATTGGTAAATTCAAGGGTATACTTCCCCACGGCAACGGGAAGTACACATGGTCAGATGGATCGGTATATGAAGGGGATTGGATAGATGGAAAAATGACAGGGAAAGGGATACTTATATGGCCATCAGGAGCAAAGTACGAGGGCGAATTCTCCGGGAATTATCTTCACGGTCACGGAACATTCACAAAATCGAACGGCTGTGTTTACACTGGTGGATGGAGGATGAATGCTCATCACGGGATTGGACGAAAAGTGTATTCCAATTCAGATGTTTACGAAGGTTTATGGAAAGAAGGAATTCGCGAAGGCAGCGGAAAATTTACTTGGAATGATGGAAGTATCTATATTGGAAATTGGAAAAAAGGGAAAATAGATGGAAGGGGGATTATGAAGTGGTTTAACGGTGATGTTTTTGATGGTTGTTGGTTAAATGGACTTAGACAAGGATCTGGAGTTTATAGATTTTCAGATGGAGGACTTTATATTGGAACATGGAGTAAAGGCCTAAAGGACGGAAAAGGAACATTCTATCCTTCTGGTAGCAAACAACCATCTCTAAAGAAATGGTGTACCAATCTTCATAGCAATGAGAATTGTAAGGTTCCAAATTCAAGATTTAACCGTAGTCTCTCTGAAAAAGCTCGAGCCAGTGGTAGATCAAAAAGTTCACGTCATTTATCTCAAAGTACTTCATTGTTGGATGAGAATTGTGTGATTCAAAACCCTGCTGACGATTGCATATGTCGTGATTCCTCATCCGCGTTATCGCAGACCTCTAATGAAGGTCAATCTGAGGTATCTGGTATGAGTAATATGGTTTTCGAAAGGGAATATATGCAAGGAGTTCTAATTGTGGAGAGACTTCGTGAGTATTCAGAAATATCGCATAATAAGAACAAAAAGGAAAGTAAATTTAGTATGAAGCAAGTAAAGAAGAGTTCAATTATAGGCACTTTCCAACACCGCCGAAGCTATTATCTGAAGCTTAATTTGCAGCTTGGCATCAGGTACACTGTAGGAAAAATTACACCGGTTCCTGCACGCGAAGTTCGGTCGTCTGATTTTGGAGAAAGAGCTAGGATAAGGATGTACTTTCCTAAGGAGGGTTCACAATTGACTCCTCCACACTGTTCTGTAGACTTCTACTGGAAAGATTATTGCCCTATGGTCTTCAGGAATTTGAGAGAAATGTTCAAATTGGACGCGGCCGAGTACATGATGTCCATTTGTGGTGATTGTGCTCTAAAGGACATATCTTCAGCTGGAAAAAGTGGCAGCATATTCTTTATTTCTCAAGATGACAAATTTGTGATCAAGACACTAAAAAAATATGAGCTAAAGGTTCTGCTCAATATGCTTCCTAAATACTACCATCATGTAGGAAGTTATGAAAATACTCTTATTACAAAATTCTTCGGGATCCATCGAATAACACTGAGAGGTGGGAAAAAGGTGCGGTTTGTTGTCATGGGAAATATGTTTTGCACAGAACTTCATATCCATCGTCGTTACGATCTGAAGGGATCTTCTCAAGGAAGATATACAAGCCAAGATAAAATTAATAGCAATACAACATTGAAAGATCTTGATCTAAAATACGAATTTCATATGGATAAAAAGTTGCGAGAATCGTTATTCAAGCAAATTTCTCTAGACTGCAAGTTCTTGGAATCTCAGCACATAATTGATTACAGTCTTCTGTTGGGATTGCATTTTAGAGCTCCA
Protein sequence

>Msa0828920

MEDNGRFEEKSFTNGDVYIGKFKGILPHGNGKYTWSDGSVYEGDWIDGKMTGKGILIWPSGAKYEGEFSGNYLHGHGTFTKSNGCVYTGGWRMNAHHGIGRKVYSNSDVYEGLWKEGIREGSGKFTWNDGSIYIGNWKKGKIDGRGIMKWFNGDVFDGCWLNGLRQGSGVYRFSDGGLYIGTWSKGLKDGKGTFYPSGSKQPSLKKWCTNLHSNENCKVPNSRFNRSLSEKARASGRSKSSRHLSQSTSLLDENCVIQNPADDCICRDSSSALSQTSNEGQSEVSGMSNMVFEREYMQGVLIVERLREYSEISHNKNKKESKFSMKQVKKSSIIGTFQHRRSYYLKLNLQLGIRYTVGKITPVPAREVRSSDFGERARIRMYFPKEGSQLTPPHCSVDFYWKDYCPMVFRNLREMFKLDAAEYMMSICGDCALKDISSAGKSGSIFFISQDDKFVIKTLKKYELKVLLNMLPKYYHHVGSYENTLITKFFGIHRITLRGGKKVRFVVMGNMFCTELHIHRRYDLKGSSQGRYTSQDKINSNTTLKDLDLKYEFHMDKKLRESLFKQISLDCKFLESQHIIDYSLLLGLHFRAP