AlfalfaGEDB | Msa0951280

M. sativa cultivar zhongmu-4 / Msa0951280

KEGG: K00130
GO: GO:0001101 GO:0003674 GO:0003824 GO:0004029 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008802 GO:0009414 GO:0009415 GO:0009507 GO:0009516 GO:0009536 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0016491 GO:0016620 GO:0016903 GO:0030312 GO:0033554 GO:0034059 GO:0036293 GO:0036294 GO:0042221 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051716 GO:0055114 GO:0070482 GO:0070887 GO:0071453 GO:0071454 GO:0071944 GO:1901700
PFAM: PF00171.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0951280	sp\|Q93YB2\|AADH2_PEA	80.189	212	13	2	53	254	311	503	4.57e-116	344

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0951280	B7FM53	83.491	212	6	2	53	254	65	257	6.03e-119	349

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003700	Msa0951280	0.810029	1.412508e-50	-8.615850e-47
Msa0046100	Msa0951280	0.800127	1.667053e-48	-8.615850e-47
Msa0091600	Msa0951280	0.823452	1.375056e-53	-8.615850e-47
Msa0146260	Msa0951280	0.819832	9.439384e-53	-8.615850e-47
Msa0195630	Msa0951280	0.802150	6.430548e-49	-8.615850e-47
Msa0283640	Msa0951280	0.815664	8.232294e-52	-8.615850e-47
Msa0333600	Msa0951280	0.842868	1.988129e-58	-8.615850e-47
Msa0546540	Msa0951280	0.868926	4.538651e-66	-8.615850e-47
Msa0731270	Msa0951280	0.865825	4.456718e-65	-8.615850e-47
Msa0771280	Msa0951280	0.864291	1.351374e-64	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0951280	MtrunA17_Chr4g0059521	83.491	212	6	2	53	254	311	503	4.07e-120	352
Msa0951280	MtrunA17_Chr3g0118121	69.058	223	37	4	43	254	302	503	5.78e-103	308
Msa0951280	MtrunA17_Chr3g0118081	68.161	223	39	4	43	254	332	533	1.76e-100	302
Msa0951280	MtrunA17_Chr6g0485311	35.897	195	94	2	61	245	326	499	9.57e-35	130
Msa0951280	MtrunA17_Chr6g0485301	35.602	191	92	3	65	245	332	501	2.13e-34	129
Msa0951280	MtrunA17_Chr8g0390631	34.500	200	100	3	56	245	315	493	2.54e-33	126
Msa0951280	MtrunA17_Chr8g0390651	34.031	191	95	3	65	245	333	502	1.27e-32	124
Msa0951280	MtrunA17_Chr8g0365221	35.079	191	93	3	65	245	371	540	7.43e-32	122
Msa0951280	MtrunA17_Chr2g0310091	36.126	191	91	2	56	236	360	529	2.58e-31	120
Msa0951280	MtrunA17_Chr3g0117861	35.079	191	93	3	65	245	372	541	2.94e-31	120

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0951280	AT3G48170.1	66.816	223	42	4	43	254	302	503	1.67e-101	305
Msa0951280	AT1G74920.2	68.182	220	38	4	43	251	297	495	6.66e-101	303
Msa0951280	AT1G74920.1	68.182	220	38	4	43	251	302	500	8.66e-101	303
Msa0951280	AT3G24503.1	38.000	200	93	3	56	245	322	500	5.60e-39	142
Msa0951280	AT1G23800.1	33.333	192	97	3	64	245	363	533	1.10e-30	119
Msa0951280	AT1G79440.1	36.364	187	88	2	60	236	355	520	1.74e-30	119
Msa0951280	AT3G48000.1	32.984	191	97	2	65	245	368	537	7.58e-30	117
Msa0951280	AT3G66658.2	29.775	178	98	3	56	227	352	508	4.70e-19	86.7
Msa0951280	AT3G66658.1	29.775	178	98	3	56	227	352	508	5.35e-19	86.3
Msa0951280	AT2G14170.3	29.114	158	95	2	56	213	410	550	2.19e-14	72.8

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Find 46 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATGTGAGCGTGTAACTA+AGG	0.508552	6_3:+41277340	Msa0951280:CDS
ACCTGTCCTTCACTAACAAC+AGG	0.463133	6_3:-41276452	None:intergenic
ACCTGTTGTTAGTGAAGGAC+AGG	0.472260	6_3:+41276451	Msa0951280:CDS
AGCCTGCAACCTTCTTCAAA+GGG	0.524637	6_3:-41276425	None:intergenic
AGTACATCTCTGATGAAACA+TGG	0.587377	6_3:+41278097	Msa0951280:CDS
AGTAGTCACATCAGTTATGA+TGG	0.526637	6_3:-41277080	None:intergenic
ATATTGCTTCAATTCATGCA+CGG	0.618950	6_3:-41275878	None:intergenic
ATCCCTTTGAAGAAGGTTGC+AGG	0.363936	6_3:+41276423	Msa0951280:CDS
ATGCTTCACTCAAGCGCCAT+GGG	0.461661	6_3:+41277488	Msa0951280:CDS
ATTTAAGGCCGGAATAGTAT+GGG	0.604605	6_3:+41277449	Msa0951280:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGAAATAGTTGGAAGTAGT+TGG	-	chr6_3:41273650-41273669	None:intergenic	30.0%
AAGACATGCAATGAATAACA+GGG	-	chr6_3:41273737-41273756	None:intergenic	30.0%
AAAGACATGCAATGAATAAC+AGG	-	chr6_3:41273738-41273757	None:intergenic	30.0%
TTTAATTGATGTTCTTCCTC+AGG	+	chr6_3:41273799-41273818	Msa0951280:CDS	30.0%
AAGCTTAAGTAATTGTGATG+TGG	+	chr6_3:41274287-41274306	Msa0951280:intron	30.0%
TTAAGTAATTGTGATGTGGT+TGG	+	chr6_3:41274291-41274310	Msa0951280:intron	30.0%
AATCACAAAGAAAAGCCATA+AGG	-	chr6_3:41274336-41274355	None:intergenic	30.0%
TGAACAAACTATATCAGTTC+CGG	-	chr6_3:41274380-41274399	None:intergenic	30.0%
GGATAAATCATTGGTGTATA+AGG	+	chr6_3:41274428-41274447	Msa0951280:intron	30.0%
GTCTTAGAATGAGATTATGA+TGG	+	chr6_3:41274454-41274473	Msa0951280:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	41273515	41278149	41273515	ID=Msa0951280;Name=Msa0951280
chr6_3	mRNA	41273515	41278149	41273515	ID=Msa0951280-mRNA-1;Parent=Msa0951280;Name=Msa0951280-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0\|0.22\|0.1\|0.5\|1\|1\|10\|0\|254
chr6_3	exon	41273515	41273520	41273515	ID=Msa0951280-mRNA-1:exon:9836;Parent=Msa0951280-mRNA-1
chr6_3	exon	41273797	41273844	41273797	ID=Msa0951280-mRNA-1:exon:9837;Parent=Msa0951280-mRNA-1
chr6_3	exon	41273986	41274009	41273986	ID=Msa0951280-mRNA-1:exon:9838;Parent=Msa0951280-mRNA-1
chr6_3	exon	41275832	41275917	41275832	ID=Msa0951280-mRNA-1:exon:9839;Parent=Msa0951280-mRNA-1
chr6_3	exon	41276388	41276472	41276388	ID=Msa0951280-mRNA-1:exon:9840;Parent=Msa0951280-mRNA-1
chr6_3	exon	41276881	41276958	41276881	ID=Msa0951280-mRNA-1:exon:9841;Parent=Msa0951280-mRNA-1
chr6_3	exon	41276992	41277200	41276992	ID=Msa0951280-mRNA-1:exon:9842;Parent=Msa0951280-mRNA-1
chr6_3	exon	41277301	41277361	41277301	ID=Msa0951280-mRNA-1:exon:9843;Parent=Msa0951280-mRNA-1
chr6_3	exon	41277447	41277528	41277447	ID=Msa0951280-mRNA-1:exon:9844;Parent=Msa0951280-mRNA-1
chr6_3	exon	41278064	41278149	41278064	ID=Msa0951280-mRNA-1:exon:9845;Parent=Msa0951280-mRNA-1
chr6_3	CDS	41273515	41273520	41273515	ID=Msa0951280-mRNA-1:cds;Parent=Msa0951280-mRNA-1
chr6_3	CDS	41273797	41273844	41273797	ID=Msa0951280-mRNA-1:cds;Parent=Msa0951280-mRNA-1
chr6_3	CDS	41273986	41274009	41273986	ID=Msa0951280-mRNA-1:cds;Parent=Msa0951280-mRNA-1
chr6_3	CDS	41275832	41275917	41275832	ID=Msa0951280-mRNA-1:cds;Parent=Msa0951280-mRNA-1
chr6_3	CDS	41276388	41276472	41276388	ID=Msa0951280-mRNA-1:cds;Parent=Msa0951280-mRNA-1
chr6_3	CDS	41276881	41276958	41276881	ID=Msa0951280-mRNA-1:cds;Parent=Msa0951280-mRNA-1
chr6_3	CDS	41276992	41277200	41276992	ID=Msa0951280-mRNA-1:cds;Parent=Msa0951280-mRNA-1
chr6_3	CDS	41277301	41277361	41277301	ID=Msa0951280-mRNA-1:cds;Parent=Msa0951280-mRNA-1
chr6_3	CDS	41277447	41277528	41277447	ID=Msa0951280-mRNA-1:cds;Parent=Msa0951280-mRNA-1
chr6_3	CDS	41278064	41278149	41278064	ID=Msa0951280-mRNA-1:cds;Parent=Msa0951280-mRNA-1

Gene Sequence

>Msa0951280

ATGAATGTTTTAATTGATGTTCTTCCTCAGGTTCGGTTGATATCAAGCTCAAAGGTTTCTTTGTTATTGAGCTCAGAGGTTTGTTATAATGTTCTGAAAATCGCCATTGACGCCATTCCAGATTCCGTGCATGAATTGAAGCAATATTTTATAAGTCTTCTCATGATGGTGAAATGGATCAAAAACATCAAAATTTCAGATCCCTTTGAAGAAGGTTGCAGGCTAGGACCTGTTGTTAGTGAAGGACAGTACAAAAAAATATTGAAGTTTGTCTCGAACGCTAAGAGTGAGGGTGCGACAATTTTGACTGGTGGGTCTCGACCAGAGTTAGGTATTTCTGCAGTTTCCATGATAATTGATTATGTTTTGCAAATGCAATGGCAGCATCTAAAGAAAGGATTCTTTGTTGAACCAACCATCATAACTGATGTGACTACTTCCATGCAAATTTGGAAAGAAGAAGTATTTGGACCTGTTCTGTGTGTAAAAACGTTTAGCACCGAGGAAGAAGCTATTGATCTAGCAAATGACACTATCTATGGCTTAGGTGCTGCTGTAATATCAAATGATCTAGAAAGATGTGAGCGTGTAACTAAGGCATTTAAGGCCGGAATAGTATGGGTCAATTGCTGTCAGCCATGCTTCACTCAAGCGCCATGGGGAGGCATTAAACGTAGTGGGGACAATTACTTGAGCGTGAAGCAAGTGACTCAGTACATCTCTGATGAAACATGGGGATGGTACCAACCTCCTGCAAAACTGTGA

Protein sequence

>Msa0951280

MNVLIDVLPQVRLISSSKVSLLLSSEVCYNVLKIAIDAIPDSVHELKQYFISLLMMVKWIKNIKISDPFEEGCRLGPVVSEGQYKKILKFVSNAKSEGATILTGGSRPELGISAVSMIIDYVLQMQWQHLKKGFFVEPTIITDVTTSMQIWKEEVFGPVLCVKTFSTEEEAIDLANDTIYGLGAAVISNDLERCERVTKAFKAGIVWVNCCQPCFTQAPWGGIKRSGDNYLSVKQVTQYISDETWGWYQPPAKL*