AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0834030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0834030 MtrunA17_Chr5g0399521 91.903 247 13 3 2 244 1 244 7.44e-149 415
Msa0834030 MtrunA17_Chr3g0126611 32.086 187 109 9 28 205 43 220 2.91e-15 74.3
Msa0834030 MtrunA17_Chr8g0347101 31.098 164 98 6 30 185 33 189 6.73e-15 73.2
Msa0834030 MtrunA17_Chr4g0037001 32.593 135 77 4 1 124 8 139 1.42e-12 67.0
Msa0834030 MtrunA17_Chr1g0158771 26.667 225 148 7 22 235 37 255 3.82e-12 65.5
Msa0834030 MtrunA17_Chr8g0388231 26.009 223 131 10 2 199 1 214 8.30e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0834030 AT5G53110.1 35.057 174 94 7 27 186 39 207 4.04e-17 80.1
Msa0834030 AT2G25410.1 28.807 243 136 10 31 242 42 278 4.14e-16 77.0
Msa0834030 AT2G46495.2 28.655 171 102 8 29 185 35 199 3.34e-13 68.6
Msa0834030 AT2G46495.1 28.655 171 102 8 29 185 35 199 3.68e-13 68.6
Msa0834030 AT5G36001.2 29.560 159 94 8 29 185 41 183 1.14e-11 62.8
Msa0834030 AT5G36001.1 29.560 159 94 8 29 185 41 183 3.96e-11 62.4

Find 59 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTTCTCACCCTCTTCT+TGG 0.138462 5_4:-7087155 Msa0834030:CDS
TATGATGATACCACTCTTTC+TGG 0.178660 5_4:-7087034 Msa0834030:CDS
AAGTTCTTCATTATCAAATT+TGG 0.203769 5_4:-7085907 Msa0834030:three_prime_UTR
TCACTATCCATTTCACATAA+AGG 0.295402 5_4:-7087080 Msa0834030:CDS
CAAGTAGACTTTGTGAAGTT+TGG 0.298271 5_4:+7087353 None:intergenic
CTTTGCATAAAGAATTAAAA+AGG 0.312037 5_4:+7087292 None:intergenic
TGTCACACAAAGAAAATATA+TGG 0.321871 5_4:+7085696 None:intergenic
AGACTGTTGTCTGAAATATA+AGG 0.324770 5_4:+7086659 None:intergenic
CACTATCCATTTCACATAAA+GGG 0.339521 5_4:-7087079 Msa0834030:CDS
GAACTAGGCATAGGAACTTC+AGG 0.345941 5_4:+7086725 None:intergenic
CATAGCGTGGCAGATTTCTA+AGG 0.365268 5_4:-7085973 Msa0834030:exon
AAGAAGAGTGAATTCATCAT+TGG 0.384353 5_4:+7087172 None:intergenic
AACGCTTCAAGCGATGTTAG+AGG 0.407833 5_4:-7085827 Msa0834030:three_prime_UTR
GTTAACTGCAACGGTATTGC+TGG 0.410536 5_4:-7087112 Msa0834030:CDS
TAAAATACGAACACTATTTG+AGG 0.411041 5_4:-7085630 Msa0834030:three_prime_UTR
TGTTGACCCTTTATGTGAAA+TGG 0.413795 5_4:+7087073 None:intergenic
GTCACGACTACATCAATCTA+AGG 0.414143 5_4:-7085662 Msa0834030:three_prime_UTR
CGTTGCAGTTAACAGAGGTT+TGG 0.417106 5_4:+7087122 None:intergenic
GGCAACACTTGTAGGCATAA+AGG 0.435949 5_4:-7086031 Msa0834030:CDS
AGGACTTCTTGGTTTCTTGT+TGG 0.440422 5_4:+7087312 None:intergenic
GACTGTTGTCTGAAATATAA+GGG 0.447474 5_4:+7086660 None:intergenic
AACATCGCTTGAAGCGTTGA+GGG 0.457835 5_4:+7085832 None:intergenic
TATATTTCAGACAACAGTCT+TGG 0.476999 5_4:-7086656 Msa0834030:CDS
GCATAAAGGTTCATTTATCA+AGG 0.478351 5_4:-7086017 Msa0834030:CDS
GTAGACTTTGTGAAGTTTGG+TGG 0.479187 5_4:+7087356 None:intergenic
GTGGCACTGGCAACACTTGT+AGG 0.486667 5_4:-7086039 Msa0834030:CDS
AGAAGAGTGAATTCATCATT+GGG 0.494871 5_4:+7087173 None:intergenic
ACTATCAAATCACCATATGA+TGG 0.503242 5_4:+7086968 None:intergenic
TGAAATGGATAGTGAATGTC+AGG 0.523321 5_4:+7087088 None:intergenic
AATCTGAAGAAAGCAAAGAA+TGG 0.524107 5_4:-7086607 Msa0834030:CDS
AGTGAATTCATCATTGGGAA+TGG 0.525024 5_4:+7087178 None:intergenic
AACTAGGCATAGGAACTTCA+GGG 0.534743 5_4:+7086726 None:intergenic
CATTTATCAAGGAGGAAAGA+AGG 0.537861 5_4:-7086006 Msa0834030:CDS
CAAAATGACCAATTCTGTAA+AGG 0.539785 5_4:+7087379 None:intergenic
TCTCACACAGCACGCAATAC+AGG 0.540563 5_4:-7086569 Msa0834030:intron
ACGGTGTAAAGGTGAGGTTA+AGG 0.543625 5_4:+7086870 None:intergenic
ATAGTATTGGAACGGTGTAA+AGG 0.555489 5_4:+7086859 None:intergenic
ACCATATGATGGAAAATGAA+TGG 0.555827 5_4:+7086979 None:intergenic
TAACATCGCTTGAAGCGTTG+AGG 0.577723 5_4:+7085831 None:intergenic
GATTTCTAAGGACATAGTAA+AGG 0.589533 5_4:-7085961 Msa0834030:exon
ATATAAGGGCTATATTTGAA+AGG 0.590983 5_4:+7086674 None:intergenic
GAAGATGAAAACCAAGAAGA+GGG 0.591458 5_4:+7087144 None:intergenic
AGGACAATTGTTGCATAGCG+TGG 0.602254 5_4:-7085986 Msa0834030:CDS
AAGCTCAAAACCAGAAAGAG+TGG 0.602365 5_4:+7087024 None:intergenic
CAAAGTACATATATTCTAGA+CGG 0.605467 5_4:-7087274 Msa0834030:five_prime_UTR
AGAACGTAATAGTATTGGAA+CGG 0.607440 5_4:+7086851 None:intergenic
GGAAGATGAAAACCAAGAAG+AGG 0.611027 5_4:+7087143 None:intergenic
CAAACCTCTGTTAACTGCAA+CGG 0.615436 5_4:-7087121 Msa0834030:CDS
ATATTTGAAAGGAATAGCAA+TGG 0.622554 5_4:+7086685 None:intergenic
GGAATGGAGAAGATGTTATG+AGG 0.622795 5_4:+7087194 None:intergenic
TAACTTGAATCACTTAAACA+TGG 0.633744 5_4:+7086767 None:intergenic
GTCTTGGTCTTAGATTAACA+TGG 0.638466 5_4:-7086640 Msa0834030:CDS
AAAGTCTTGTGGAGCAATTG+AGG 0.638737 5_4:+7086813 None:intergenic
TAAAGGTTCATTTATCAAGG+AGG 0.641975 5_4:-7086014 Msa0834030:CDS
ATTGGAACGGTGTAAAGGTG+AGG 0.642041 5_4:+7086864 None:intergenic
TCTTGGTCTTAGATTAACAT+GGG 0.645510 5_4:-7086639 Msa0834030:CDS
AATACCGTTGCAGTTAACAG+AGG 0.663218 5_4:+7087117 None:intergenic
TAAGGTTGAGAAAGACTCTG+TGG 0.692883 5_4:+7086888 None:intergenic
ATATGATGGAAAATGAATGG+TGG 0.729816 5_4:+7086982 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTACAAAAATAAAGTTCA+TGG - chr5_4:7086517-7086536 Msa0834030:intron 15.0%
!! ATTACAAAAATAAAGTTCAT+GGG - chr5_4:7086518-7086537 Msa0834030:intron 15.0%
!! TGTATAGTACATATATATAT+TGG - chr5_4:7086653-7086672 Msa0834030:CDS 15.0%
!! GTATAGTACATATATATATT+GGG - chr5_4:7086654-7086673 Msa0834030:CDS 15.0%
!! AATTACAAAAATAAAGTTCA+TGG - chr5_4:7086517-7086536 Msa0834030:intron 15.0%
!! ATTACAAAAATAAAGTTCAT+GGG - chr5_4:7086518-7086537 Msa0834030:intron 15.0%
!! TGTATAGTACATATATATAT+TGG - chr5_4:7086653-7086672 Msa0834030:CDS 15.0%
!! GTATAGTACATATATATATT+GGG - chr5_4:7086654-7086673 Msa0834030:CDS 15.0%
!! CTTTGCATAAAGAATTAAAA+AGG + chr5_4:7085749-7085768 None:intergenic 20.0%
!!! TTTTACTTCTTATTCATTCT+TGG - chr5_4:7085797-7085816 Msa0834030:three_prime_UTR 20.0%
!!! TTTTAAGAACGTAATAGTAT+TGG + chr5_4:7086195-7086214 None:intergenic 20.0%
!! TTTGTAATTAAAGAAAGAGA+TGG + chr5_4:7086506-7086525 None:intergenic 20.0%
!! AGAAAATTATTAACAAAGCT+TGG - chr5_4:7086881-7086900 Msa0834030:CDS 20.0%
!! AAGTTCTTCATTATCAAATT+TGG - chr5_4:7087131-7087150 Msa0834030:CDS 20.0%
!!! TTTTGTATAGTATATGTTGT+AGG - chr5_4:7087165-7087184 Msa0834030:CDS 20.0%
!! CTTTGCATAAAGAATTAAAA+AGG + chr5_4:7085749-7085768 None:intergenic 20.0%
!!! TTTTACTTCTTATTCATTCT+TGG - chr5_4:7085797-7085816 Msa0834030:three_prime_UTR 20.0%
!!! TTTTAAGAACGTAATAGTAT+TGG + chr5_4:7086195-7086214 None:intergenic 20.0%
!! TTTGTAATTAAAGAAAGAGA+TGG + chr5_4:7086506-7086525 None:intergenic 20.0%
!! AGAAAATTATTAACAAAGCT+TGG - chr5_4:7086881-7086900 Msa0834030:CDS 20.0%
!! AAGTTCTTCATTATCAAATT+TGG - chr5_4:7087131-7087150 Msa0834030:CDS 20.0%
!!! TTTTGTATAGTATATGTTGT+AGG - chr5_4:7087165-7087184 Msa0834030:CDS 20.0%
! AAGAATTAAAAAGGACTTCT+TGG + chr5_4:7085740-7085759 None:intergenic 25.0%
! CAAAGTACATATATTCTAGA+CGG - chr5_4:7085764-7085783 Msa0834030:three_prime_UTR 25.0%
!! ATCAAGAAGATCAATCTTTT+TGG + chr5_4:7086116-7086135 None:intergenic 25.0%
! TAACTTGAATCACTTAAACA+TGG + chr5_4:7086274-7086293 None:intergenic 25.0%
!!! TTGACTTTTTTACATGAACT+AGG + chr5_4:7086331-7086350 None:intergenic 25.0%
! ATATTTGAAAGGAATAGCAA+TGG + chr5_4:7086356-7086375 None:intergenic 25.0%
! ATATAAGGGCTATATTTGAA+AGG + chr5_4:7086367-7086386 None:intergenic 25.0%
! AGGAAATTATGCTCATTTAA+AGG + chr5_4:7086562-7086581 None:intergenic 25.0%
! ATAATTTCCTTATACTAGGT+TGG - chr5_4:7086572-7086591 Msa0834030:CDS 25.0%
! TAATTTCCTTATACTAGGTT+GGG - chr5_4:7086573-7086592 Msa0834030:CDS 25.0%
! AGTACATATATATATTGGGA+AGG - chr5_4:7086658-7086677 Msa0834030:CDS 25.0%
! ATCATTACGAAGTATATACT+AGG - chr5_4:7086712-7086731 Msa0834030:CDS 25.0%
! TGTCACACAAAGAAAATATA+TGG + chr5_4:7087345-7087364 None:intergenic 25.0%
! TAAAATACGAACACTATTTG+AGG - chr5_4:7087408-7087427 Msa0834030:five_prime_UTR 25.0%
! AAGAATTAAAAAGGACTTCT+TGG + chr5_4:7085740-7085759 None:intergenic 25.0%
! CAAAGTACATATATTCTAGA+CGG - chr5_4:7085764-7085783 Msa0834030:three_prime_UTR 25.0%
!! ATCAAGAAGATCAATCTTTT+TGG + chr5_4:7086116-7086135 None:intergenic 25.0%
! TAACTTGAATCACTTAAACA+TGG + chr5_4:7086274-7086293 None:intergenic 25.0%
!!! TTGACTTTTTTACATGAACT+AGG + chr5_4:7086331-7086350 None:intergenic 25.0%
! ATATTTGAAAGGAATAGCAA+TGG + chr5_4:7086356-7086375 None:intergenic 25.0%
! ATATAAGGGCTATATTTGAA+AGG + chr5_4:7086367-7086386 None:intergenic 25.0%
! AGGAAATTATGCTCATTTAA+AGG + chr5_4:7086562-7086581 None:intergenic 25.0%
! ATAATTTCCTTATACTAGGT+TGG - chr5_4:7086572-7086591 Msa0834030:CDS 25.0%
! TAATTTCCTTATACTAGGTT+GGG - chr5_4:7086573-7086592 Msa0834030:CDS 25.0%
! AGTACATATATATATTGGGA+AGG - chr5_4:7086658-7086677 Msa0834030:CDS 25.0%
! ATCATTACGAAGTATATACT+AGG - chr5_4:7086712-7086731 Msa0834030:CDS 25.0%
! TGTCACACAAAGAAAATATA+TGG + chr5_4:7087345-7087364 None:intergenic 25.0%
! TAAAATACGAACACTATTTG+AGG - chr5_4:7087408-7087427 Msa0834030:five_prime_UTR 25.0%
! TTTTTGCTCCTTTACAGAAT+TGG - chr5_4:7085651-7085670 Msa0834030:three_prime_UTR 30.0%
CAAAATGACCAATTCTGTAA+AGG + chr5_4:7085662-7085681 None:intergenic 30.0%
AGAAGAGTGAATTCATCATT+GGG + chr5_4:7085868-7085887 None:intergenic 30.0%
AAGAAGAGTGAATTCATCAT+TGG + chr5_4:7085869-7085888 None:intergenic 30.0%
TCACTATCCATTTCACATAA+AGG - chr5_4:7085958-7085977 Msa0834030:exon 30.0%
CACTATCCATTTCACATAAA+GGG - chr5_4:7085959-7085978 Msa0834030:exon 30.0%
! ATATGATGGAAAATGAATGG+TGG + chr5_4:7086059-7086078 None:intergenic 30.0%
! ACCATTCATTTTCCATCATA+TGG - chr5_4:7086058-7086077 Msa0834030:CDS 30.0%
! ACCATATGATGGAAAATGAA+TGG + chr5_4:7086062-7086081 None:intergenic 30.0%
ACTATCAAATCACCATATGA+TGG + chr5_4:7086073-7086092 None:intergenic 30.0%
!!! ATCAATCTTTTTGGTGTCAT+AGG + chr5_4:7086107-7086126 None:intergenic 30.0%
! AGAACGTAATAGTATTGGAA+CGG + chr5_4:7086190-7086209 None:intergenic 30.0%
!! TTTTGTTTGTGAAAGTCTTG+TGG + chr5_4:7086239-7086258 None:intergenic 30.0%
! TTTTTACATGAACTAGGCAT+AGG + chr5_4:7086325-7086344 None:intergenic 30.0%
! GACTGTTGTCTGAAATATAA+GGG + chr5_4:7086381-7086400 None:intergenic 30.0%
! AGACTGTTGTCTGAAATATA+AGG + chr5_4:7086382-7086401 None:intergenic 30.0%
TATATTTCAGACAACAGTCT+TGG - chr5_4:7086382-7086401 Msa0834030:intron 30.0%
!! TCTTGGTCTTAGATTAACAT+GGG - chr5_4:7086399-7086418 Msa0834030:intron 30.0%
AATCTGAAGAAAGCAAAGAA+TGG - chr5_4:7086431-7086450 Msa0834030:intron 30.0%
GAGCATAATTTCCTTATACT+AGG - chr5_4:7086568-7086587 Msa0834030:intron 30.0%
TTACGAAGTATATACTAGGT+TGG - chr5_4:7086716-7086735 Msa0834030:CDS 30.0%
TACGAAGTATATACTAGGTT+GGG - chr5_4:7086717-7086736 Msa0834030:CDS 30.0%
TAGTACATTATCTTTCGTGT+AGG - chr5_4:7086779-7086798 Msa0834030:CDS 30.0%
GCATAAAGGTTCATTTATCA+AGG - chr5_4:7087021-7087040 Msa0834030:CDS 30.0%
TAAAGGTTCATTTATCAAGG+AGG - chr5_4:7087024-7087043 Msa0834030:CDS 30.0%
! GATTTCTAAGGACATAGTAA+AGG - chr5_4:7087077-7087096 Msa0834030:CDS 30.0%
AGTGTCTAAACTTCAATGTT+GGG + chr5_4:7085597-7085616 None:intergenic 30.0%
AAGTGTCTAAACTTCAATGT+TGG + chr5_4:7085598-7085617 None:intergenic 30.0%
! TTTTTGCTCCTTTACAGAAT+TGG - chr5_4:7085651-7085670 Msa0834030:three_prime_UTR 30.0%
CAAAATGACCAATTCTGTAA+AGG + chr5_4:7085662-7085681 None:intergenic 30.0%
AGAAGAGTGAATTCATCATT+GGG + chr5_4:7085868-7085887 None:intergenic 30.0%
AAGAAGAGTGAATTCATCAT+TGG + chr5_4:7085869-7085888 None:intergenic 30.0%
TCACTATCCATTTCACATAA+AGG - chr5_4:7085958-7085977 Msa0834030:exon 30.0%
CACTATCCATTTCACATAAA+GGG - chr5_4:7085959-7085978 Msa0834030:exon 30.0%
! ATATGATGGAAAATGAATGG+TGG + chr5_4:7086059-7086078 None:intergenic 30.0%
! ACCATTCATTTTCCATCATA+TGG - chr5_4:7086058-7086077 Msa0834030:CDS 30.0%
! ACCATATGATGGAAAATGAA+TGG + chr5_4:7086062-7086081 None:intergenic 30.0%
ACTATCAAATCACCATATGA+TGG + chr5_4:7086073-7086092 None:intergenic 30.0%
!!! ATCAATCTTTTTGGTGTCAT+AGG + chr5_4:7086107-7086126 None:intergenic 30.0%
! AGAACGTAATAGTATTGGAA+CGG + chr5_4:7086190-7086209 None:intergenic 30.0%
!! TTTTGTTTGTGAAAGTCTTG+TGG + chr5_4:7086239-7086258 None:intergenic 30.0%
! TTTTTACATGAACTAGGCAT+AGG + chr5_4:7086325-7086344 None:intergenic 30.0%
! GACTGTTGTCTGAAATATAA+GGG + chr5_4:7086381-7086400 None:intergenic 30.0%
! AGACTGTTGTCTGAAATATA+AGG + chr5_4:7086382-7086401 None:intergenic 30.0%
TATATTTCAGACAACAGTCT+TGG - chr5_4:7086382-7086401 Msa0834030:intron 30.0%
!! TCTTGGTCTTAGATTAACAT+GGG - chr5_4:7086399-7086418 Msa0834030:intron 30.0%
AATCTGAAGAAAGCAAAGAA+TGG - chr5_4:7086431-7086450 Msa0834030:intron 30.0%
GAGCATAATTTCCTTATACT+AGG - chr5_4:7086568-7086587 Msa0834030:intron 30.0%
TTACGAAGTATATACTAGGT+TGG - chr5_4:7086716-7086735 Msa0834030:CDS 30.0%
TACGAAGTATATACTAGGTT+GGG - chr5_4:7086717-7086736 Msa0834030:CDS 30.0%
TAGTACATTATCTTTCGTGT+AGG - chr5_4:7086779-7086798 Msa0834030:CDS 30.0%
GCATAAAGGTTCATTTATCA+AGG - chr5_4:7087021-7087040 Msa0834030:CDS 30.0%
TAAAGGTTCATTTATCAAGG+AGG - chr5_4:7087024-7087043 Msa0834030:CDS 30.0%
! GATTTCTAAGGACATAGTAA+AGG - chr5_4:7087077-7087096 Msa0834030:CDS 30.0%
CAAGTAGACTTTGTGAAGTT+TGG + chr5_4:7085688-7085707 None:intergenic 35.0%
AGTGAATTCATCATTGGGAA+TGG + chr5_4:7085863-7085882 None:intergenic 35.0%
GAAGATGAAAACCAAGAAGA+GGG + chr5_4:7085897-7085916 None:intergenic 35.0%
TGAAATGGATAGTGAATGTC+AGG + chr5_4:7085953-7085972 None:intergenic 35.0%
TGTTGACCCTTTATGTGAAA+TGG + chr5_4:7085968-7085987 None:intergenic 35.0%
TATGATGATACCACTCTTTC+TGG - chr5_4:7086004-7086023 Msa0834030:CDS 35.0%
!!! CTTTTTGGTGTCATAGGAAA+TGG + chr5_4:7086101-7086120 None:intergenic 35.0%
! ATAGTATTGGAACGGTGTAA+AGG + chr5_4:7086182-7086201 None:intergenic 35.0%
!! GTCTTGGTCTTAGATTAACA+TGG - chr5_4:7086398-7086417 Msa0834030:intron 35.0%
AAACTACCCAACCTAGTATA+AGG + chr5_4:7086582-7086601 None:intergenic 35.0%
! ATATACTAGGTTGGGTAGTT+TGG - chr5_4:7086725-7086744 Msa0834030:CDS 35.0%
TCTTTCGTGTAGGATTGATT+CGG - chr5_4:7086789-7086808 Msa0834030:CDS 35.0%
CATTTATCAAGGAGGAAAGA+AGG - chr5_4:7087032-7087051 Msa0834030:CDS 35.0%
GTGTCTAAACTTCAATGTTG+GGG + chr5_4:7085596-7085615 None:intergenic 35.0%
CAAGTAGACTTTGTGAAGTT+TGG + chr5_4:7085688-7085707 None:intergenic 35.0%
AGTGAATTCATCATTGGGAA+TGG + chr5_4:7085863-7085882 None:intergenic 35.0%
GAAGATGAAAACCAAGAAGA+GGG + chr5_4:7085897-7085916 None:intergenic 35.0%
TGAAATGGATAGTGAATGTC+AGG + chr5_4:7085953-7085972 None:intergenic 35.0%
TGTTGACCCTTTATGTGAAA+TGG + chr5_4:7085968-7085987 None:intergenic 35.0%
TATGATGATACCACTCTTTC+TGG - chr5_4:7086004-7086023 Msa0834030:CDS 35.0%
!!! CTTTTTGGTGTCATAGGAAA+TGG + chr5_4:7086101-7086120 None:intergenic 35.0%
! ATAGTATTGGAACGGTGTAA+AGG + chr5_4:7086182-7086201 None:intergenic 35.0%
!! GTCTTGGTCTTAGATTAACA+TGG - chr5_4:7086398-7086417 Msa0834030:intron 35.0%
AAACTACCCAACCTAGTATA+AGG + chr5_4:7086582-7086601 None:intergenic 35.0%
! ATATACTAGGTTGGGTAGTT+TGG - chr5_4:7086725-7086744 Msa0834030:CDS 35.0%
TCTTTCGTGTAGGATTGATT+CGG - chr5_4:7086789-7086808 Msa0834030:CDS 35.0%
CATTTATCAAGGAGGAAAGA+AGG - chr5_4:7087032-7087051 Msa0834030:CDS 35.0%
GTAGACTTTGTGAAGTTTGG+TGG + chr5_4:7085685-7085704 None:intergenic 40.0%
!! TGTTGGCTTCAAAAAGCTCA+GGG + chr5_4:7085712-7085731 None:intergenic 40.0%
!! TTGTTGGCTTCAAAAAGCTC+AGG + chr5_4:7085713-7085732 None:intergenic 40.0%
AGGACTTCTTGGTTTCTTGT+TGG + chr5_4:7085729-7085748 None:intergenic 40.0%
GGAATGGAGAAGATGTTATG+AGG + chr5_4:7085847-7085866 None:intergenic 40.0%
GGAAGATGAAAACCAAGAAG+AGG + chr5_4:7085898-7085917 None:intergenic 40.0%
CAAACCTCTGTTAACTGCAA+CGG - chr5_4:7085917-7085936 Msa0834030:three_prime_UTR 40.0%
AATACCGTTGCAGTTAACAG+AGG + chr5_4:7085924-7085943 None:intergenic 40.0%
AAGCTCAAAACCAGAAAGAG+TGG + chr5_4:7086017-7086036 None:intergenic 40.0%
!!! ACTCTGTGGACACAGTTTTT+TGG + chr5_4:7086139-7086158 None:intergenic 40.0%
TAAGGTTGAGAAAGACTCTG+TGG + chr5_4:7086153-7086172 None:intergenic 40.0%
! AAAGTCTTGTGGAGCAATTG+AGG + chr5_4:7086228-7086247 None:intergenic 40.0%
AACTAGGCATAGGAACTTCA+GGG + chr5_4:7086315-7086334 None:intergenic 40.0%
TTCAACACATGTGCAGTTCT+TGG - chr5_4:7086954-7086973 Msa0834030:CDS 40.0%
! TCTCAGCATCTGTATTTTCG+TGG - chr5_4:7086977-7086996 Msa0834030:CDS 40.0%
GTCACGACTACATCAATCTA+AGG - chr5_4:7087376-7087395 Msa0834030:five_prime_UTR 40.0%
GTAGACTTTGTGAAGTTTGG+TGG + chr5_4:7085685-7085704 None:intergenic 40.0%
!! TGTTGGCTTCAAAAAGCTCA+GGG + chr5_4:7085712-7085731 None:intergenic 40.0%
!! TTGTTGGCTTCAAAAAGCTC+AGG + chr5_4:7085713-7085732 None:intergenic 40.0%
AGGACTTCTTGGTTTCTTGT+TGG + chr5_4:7085729-7085748 None:intergenic 40.0%
GGAATGGAGAAGATGTTATG+AGG + chr5_4:7085847-7085866 None:intergenic 40.0%
GGAAGATGAAAACCAAGAAG+AGG + chr5_4:7085898-7085917 None:intergenic 40.0%
CAAACCTCTGTTAACTGCAA+CGG - chr5_4:7085917-7085936 Msa0834030:three_prime_UTR 40.0%
AATACCGTTGCAGTTAACAG+AGG + chr5_4:7085924-7085943 None:intergenic 40.0%
AAGCTCAAAACCAGAAAGAG+TGG + chr5_4:7086017-7086036 None:intergenic 40.0%
!!! ACTCTGTGGACACAGTTTTT+TGG + chr5_4:7086139-7086158 None:intergenic 40.0%
TAAGGTTGAGAAAGACTCTG+TGG + chr5_4:7086153-7086172 None:intergenic 40.0%
! AAAGTCTTGTGGAGCAATTG+AGG + chr5_4:7086228-7086247 None:intergenic 40.0%
AACTAGGCATAGGAACTTCA+GGG + chr5_4:7086315-7086334 None:intergenic 40.0%
TTCAACACATGTGCAGTTCT+TGG - chr5_4:7086954-7086973 Msa0834030:CDS 40.0%
! TCTCAGCATCTGTATTTTCG+TGG - chr5_4:7086977-7086996 Msa0834030:CDS 40.0%
GTCACGACTACATCAATCTA+AGG - chr5_4:7087376-7087395 Msa0834030:five_prime_UTR 40.0%
CTTCTTTCTCACCCTCTTCT+TGG - chr5_4:7085883-7085902 Msa0834030:three_prime_UTR 45.0%
CGTTGCAGTTAACAGAGGTT+TGG + chr5_4:7085919-7085938 None:intergenic 45.0%
GTTAACTGCAACGGTATTGC+TGG - chr5_4:7085926-7085945 Msa0834030:three_prime_UTR 45.0%
ACGGTGTAAAGGTGAGGTTA+AGG + chr5_4:7086171-7086190 None:intergenic 45.0%
ATTGGAACGGTGTAAAGGTG+AGG + chr5_4:7086177-7086196 None:intergenic 45.0%
GAACTAGGCATAGGAACTTC+AGG + chr5_4:7086316-7086335 None:intergenic 45.0%
! CAGCATCTGTATTTTCGTGG+TGG - chr5_4:7086980-7086999 Msa0834030:CDS 45.0%
GGCAACACTTGTAGGCATAA+AGG - chr5_4:7087007-7087026 Msa0834030:CDS 45.0%
AGGACAATTGTTGCATAGCG+TGG - chr5_4:7087052-7087071 Msa0834030:CDS 45.0%
CATAGCGTGGCAGATTTCTA+AGG - chr5_4:7087065-7087084 Msa0834030:CDS 45.0%
!! AACATCGCTTGAAGCGTTGA+GGG + chr5_4:7087209-7087228 None:intergenic 45.0%
!! TAACATCGCTTGAAGCGTTG+AGG + chr5_4:7087210-7087229 None:intergenic 45.0%
AACGCTTCAAGCGATGTTAG+AGG - chr5_4:7087211-7087230 Msa0834030:five_prime_UTR 45.0%
CTTCTTTCTCACCCTCTTCT+TGG - chr5_4:7085883-7085902 Msa0834030:three_prime_UTR 45.0%
CGTTGCAGTTAACAGAGGTT+TGG + chr5_4:7085919-7085938 None:intergenic 45.0%
GTTAACTGCAACGGTATTGC+TGG - chr5_4:7085926-7085945 Msa0834030:three_prime_UTR 45.0%
ACGGTGTAAAGGTGAGGTTA+AGG + chr5_4:7086171-7086190 None:intergenic 45.0%
ATTGGAACGGTGTAAAGGTG+AGG + chr5_4:7086177-7086196 None:intergenic 45.0%
GAACTAGGCATAGGAACTTC+AGG + chr5_4:7086316-7086335 None:intergenic 45.0%
! CAGCATCTGTATTTTCGTGG+TGG - chr5_4:7086980-7086999 Msa0834030:CDS 45.0%
GGCAACACTTGTAGGCATAA+AGG - chr5_4:7087007-7087026 Msa0834030:CDS 45.0%
AGGACAATTGTTGCATAGCG+TGG - chr5_4:7087052-7087071 Msa0834030:CDS 45.0%
CATAGCGTGGCAGATTTCTA+AGG - chr5_4:7087065-7087084 Msa0834030:CDS 45.0%
!! AACATCGCTTGAAGCGTTGA+GGG + chr5_4:7087209-7087228 None:intergenic 45.0%
!! TAACATCGCTTGAAGCGTTG+AGG + chr5_4:7087210-7087229 None:intergenic 45.0%
AACGCTTCAAGCGATGTTAG+AGG - chr5_4:7087211-7087230 Msa0834030:five_prime_UTR 45.0%
TCTCACACAGCACGCAATAC+AGG - chr5_4:7086469-7086488 Msa0834030:intron 50.0%
! CTGTATTTTCGTGGTGGCAC+TGG - chr5_4:7086986-7087005 Msa0834030:CDS 50.0%
TCTCACACAGCACGCAATAC+AGG - chr5_4:7086469-7086488 Msa0834030:intron 50.0%
! CTGTATTTTCGTGGTGGCAC+TGG - chr5_4:7086986-7087005 Msa0834030:CDS 50.0%
!! GTGGCACTGGCAACACTTGT+AGG - chr5_4:7086999-7087018 Msa0834030:CDS 55.0%
!! GTGGCACTGGCAACACTTGT+AGG - chr5_4:7086999-7087018 Msa0834030:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 7085596 7087464 7085596 ID=Msa0834030;Name=Msa0834030
chr5_4 mRNA 7085596 7087464 7085596 ID=Msa0834030-mRNA-1;Parent=Msa0834030;Name=Msa0834030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=273|1|1|1|1|1|2|379|245
chr5_4 exon 7085596 7086090 7085596 ID=Msa0834030-mRNA-1:exon:3131;Parent=Msa0834030-mRNA-1
chr5_4 exon 7086570 7087464 7086570 ID=Msa0834030-mRNA-1:exon:3130;Parent=Msa0834030-mRNA-1
chr5_4 five_prime_UTR 7087192 7087464 7087192 ID=Msa0834030-mRNA-1:five_prime_utr;Parent=Msa0834030-mRNA-1
chr5_4 CDS 7086570 7087191 7086570 ID=Msa0834030-mRNA-1:cds;Parent=Msa0834030-mRNA-1
chr5_4 CDS 7085975 7086090 7085975 ID=Msa0834030-mRNA-1:cds;Parent=Msa0834030-mRNA-1
chr5_4 three_prime_UTR 7085596 7085974 7085596 ID=Msa0834030-mRNA-1:three_prime_utr;Parent=Msa0834030-mRNA-1
Gene Sequence

>Msa0834030

ATGATGAATTCACTCTTCTTTCTCACCCTCTTCTTGGTTTTCATCTTCCAAACCTCTGTTAACTGCAACGGTATTGCTGGTCCTGACATTCACTATCCATTTCACATAAAGGGTCAACAGCAACAACAACAAGAGTATGATGATACCACTCTTTCTGGTTTTGAGCTTCTTCGCAAAGACAACATCACCACCATTCATTTTCCATCATATGGTGATTTGATAGTCAAGTCCATTTCCTATGACACCAAAAAGATTGATCTTCTTGATCCAAAAAACTGTGTCCACAGAGTCTTTCTCAACCTTAACCTCACCTTTACACCGTTCCAATACTATTACGTTCTTAAAAACTTCACATACCTCAATTGCTCCACAAGACTTTCACAAACAAAATTCATTGAAGTTCCATGTTTAAGTGATTCAAGTTATCATGTTTACACAGTTGACCCTGAAGTTCCTATGCCTAGTTCATGTAAAAAAGTCAAAACCATTGCTATTCCTTTCAAATATAGCCCTTATATTTCAGACAACAGTCTTGGTCTTAGATTAACATGGGACTTGCGTGAATCTGAAGAAAGCAAAGAATGGAACAAGACTAGAGATTCTCACACAGCACGCAATACAGTTCTTGGTCTCAGCATCTGTATTTTCGTGGTGGCACTGGCAACACTTGTAGGCATAAAGGTTCATTTATCAAGGAGGAAAGAAGGACAATTGTTGCATAGCGTGGCAGATTTCTAA
Protein sequence

>Msa0834030

MMNSLFFLTLFLVFIFQTSVNCNGIAGPDIHYPFHIKGQQQQQQEYDDTTLSGFELLRKDNITTIHFPSYGDLIVKSISYDTKKIDLLDPKNCVHRVFLNLNLTFTPFQYYYVLKNFTYLNCSTRLSQTKFIEVPCLSDSSYHVYTVDPEVPMPSSCKKVKTIAIPFKYSPYISDNSLGLRLTWDLRESEESKEWNKTRDSHTARNTVLGLSICIFVVALATLVGIKVHLSRRKEGQLLHSVADF*