AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0091930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0091930 MtrunA17_Chr5g0399521 91.903 247 13 3 2 244 1 244 7.44e-149 415
Msa0091930 MtrunA17_Chr3g0126611 32.086 187 109 9 28 205 43 220 2.91e-15 74.3
Msa0091930 MtrunA17_Chr8g0347101 31.098 164 98 6 30 185 33 189 6.73e-15 73.2
Msa0091930 MtrunA17_Chr4g0037001 32.593 135 77 4 1 124 8 139 1.42e-12 67.0
Msa0091930 MtrunA17_Chr1g0158771 26.667 225 148 7 22 235 37 255 3.82e-12 65.5
Msa0091930 MtrunA17_Chr8g0388231 26.009 223 131 10 2 199 1 214 8.30e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0091930 AT5G53110.1 35.057 174 94 7 27 186 39 207 4.04e-17 80.1
Msa0091930 AT2G25410.1 28.807 243 136 10 31 242 42 278 4.14e-16 77.0
Msa0091930 AT2G46495.2 28.655 171 102 8 29 185 35 199 3.34e-13 68.6
Msa0091930 AT2G46495.1 28.655 171 102 8 29 185 35 199 3.68e-13 68.6
Msa0091930 AT5G36001.2 29.560 159 94 8 29 185 41 183 1.14e-11 62.8
Msa0091930 AT5G36001.1 29.560 159 94 8 29 185 41 183 3.96e-11 62.4

Find 59 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTTCTCACCCTCTTCT+TGG 0.138462 1_2:-74144705 Msa0091930:CDS
TATGATGATACCACTCTTTC+TGG 0.178660 1_2:-74144584 Msa0091930:CDS
AAGTTCTTCATTATCAAATT+TGG 0.203769 1_2:-74143457 Msa0091930:three_prime_UTR
TCACTATCCATTTCACATAA+AGG 0.295402 1_2:-74144630 Msa0091930:CDS
CAAGTAGACTTTGTGAAGTT+TGG 0.298271 1_2:+74144903 None:intergenic
CTTTGCATAAAGAATTAAAA+AGG 0.312037 1_2:+74144842 None:intergenic
TGTCACACAAAGAAAATATA+TGG 0.321871 1_2:+74143246 None:intergenic
AGACTGTTGTCTGAAATATA+AGG 0.324770 1_2:+74144209 None:intergenic
CACTATCCATTTCACATAAA+GGG 0.339521 1_2:-74144629 Msa0091930:CDS
GAACTAGGCATAGGAACTTC+AGG 0.345941 1_2:+74144275 None:intergenic
CATAGCGTGGCAGATTTCTA+AGG 0.365268 1_2:-74143523 Msa0091930:exon
AAGAAGAGTGAATTCATCAT+TGG 0.384353 1_2:+74144722 None:intergenic
AACGCTTCAAGCGATGTTAG+AGG 0.407833 1_2:-74143377 Msa0091930:three_prime_UTR
GTTAACTGCAACGGTATTGC+TGG 0.410536 1_2:-74144662 Msa0091930:CDS
TAAAATACGAACACTATTTG+AGG 0.411041 1_2:-74143180 Msa0091930:three_prime_UTR
TGTTGACCCTTTATGTGAAA+TGG 0.413795 1_2:+74144623 None:intergenic
GTCACGACTACATCAATCTA+AGG 0.414143 1_2:-74143212 Msa0091930:three_prime_UTR
CGTTGCAGTTAACAGAGGTT+TGG 0.417106 1_2:+74144672 None:intergenic
GGCAACACTTGTAGGCATAA+AGG 0.435949 1_2:-74143581 Msa0091930:CDS
AGGACTTCTTGGTTTCTTGT+TGG 0.440422 1_2:+74144862 None:intergenic
GACTGTTGTCTGAAATATAA+GGG 0.447474 1_2:+74144210 None:intergenic
AACATCGCTTGAAGCGTTGA+GGG 0.457835 1_2:+74143382 None:intergenic
TATATTTCAGACAACAGTCT+TGG 0.476999 1_2:-74144206 Msa0091930:CDS
GCATAAAGGTTCATTTATCA+AGG 0.478351 1_2:-74143567 Msa0091930:CDS
GTAGACTTTGTGAAGTTTGG+TGG 0.479187 1_2:+74144906 None:intergenic
GTGGCACTGGCAACACTTGT+AGG 0.486667 1_2:-74143589 Msa0091930:CDS
AGAAGAGTGAATTCATCATT+GGG 0.494871 1_2:+74144723 None:intergenic
ACTATCAAATCACCATATGA+TGG 0.503242 1_2:+74144518 None:intergenic
TGAAATGGATAGTGAATGTC+AGG 0.523321 1_2:+74144638 None:intergenic
AATCTGAAGAAAGCAAAGAA+TGG 0.524107 1_2:-74144157 Msa0091930:CDS
AGTGAATTCATCATTGGGAA+TGG 0.525024 1_2:+74144728 None:intergenic
AACTAGGCATAGGAACTTCA+GGG 0.534743 1_2:+74144276 None:intergenic
CATTTATCAAGGAGGAAAGA+AGG 0.537861 1_2:-74143556 Msa0091930:CDS
CAAAATGACCAATTCTGTAA+AGG 0.539785 1_2:+74144929 None:intergenic
TCTCACACAGCACGCAATAC+AGG 0.540563 1_2:-74144119 Msa0091930:intron
ACGGTGTAAAGGTGAGGTTA+AGG 0.543625 1_2:+74144420 None:intergenic
ATAGTATTGGAACGGTGTAA+AGG 0.555489 1_2:+74144409 None:intergenic
ACCATATGATGGAAAATGAA+TGG 0.555827 1_2:+74144529 None:intergenic
TAACATCGCTTGAAGCGTTG+AGG 0.577723 1_2:+74143381 None:intergenic
GATTTCTAAGGACATAGTAA+AGG 0.589533 1_2:-74143511 Msa0091930:exon
ATATAAGGGCTATATTTGAA+AGG 0.590983 1_2:+74144224 None:intergenic
GAAGATGAAAACCAAGAAGA+GGG 0.591458 1_2:+74144694 None:intergenic
AGGACAATTGTTGCATAGCG+TGG 0.602254 1_2:-74143536 Msa0091930:CDS
AAGCTCAAAACCAGAAAGAG+TGG 0.602365 1_2:+74144574 None:intergenic
CAAAGTACATATATTCTAGA+CGG 0.605467 1_2:-74144824 Msa0091930:five_prime_UTR
AGAACGTAATAGTATTGGAA+CGG 0.607440 1_2:+74144401 None:intergenic
GGAAGATGAAAACCAAGAAG+AGG 0.611027 1_2:+74144693 None:intergenic
CAAACCTCTGTTAACTGCAA+CGG 0.615436 1_2:-74144671 Msa0091930:CDS
ATATTTGAAAGGAATAGCAA+TGG 0.622554 1_2:+74144235 None:intergenic
GGAATGGAGAAGATGTTATG+AGG 0.622795 1_2:+74144744 None:intergenic
TAACTTGAATCACTTAAACA+TGG 0.633744 1_2:+74144317 None:intergenic
GTCTTGGTCTTAGATTAACA+TGG 0.638466 1_2:-74144190 Msa0091930:CDS
AAAGTCTTGTGGAGCAATTG+AGG 0.638737 1_2:+74144363 None:intergenic
TAAAGGTTCATTTATCAAGG+AGG 0.641975 1_2:-74143564 Msa0091930:CDS
ATTGGAACGGTGTAAAGGTG+AGG 0.642041 1_2:+74144414 None:intergenic
TCTTGGTCTTAGATTAACAT+GGG 0.645510 1_2:-74144189 Msa0091930:CDS
AATACCGTTGCAGTTAACAG+AGG 0.663218 1_2:+74144667 None:intergenic
TAAGGTTGAGAAAGACTCTG+TGG 0.692883 1_2:+74144438 None:intergenic
ATATGATGGAAAATGAATGG+TGG 0.729816 1_2:+74144532 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTACAAAAATAAAGTTCA+TGG - chr1_2:74144067-74144086 Msa0091930:intron 15.0%
!! ATTACAAAAATAAAGTTCAT+GGG - chr1_2:74144068-74144087 Msa0091930:intron 15.0%
!! TGTATAGTACATATATATAT+TGG - chr1_2:74144203-74144222 Msa0091930:CDS 15.0%
!! GTATAGTACATATATATATT+GGG - chr1_2:74144204-74144223 Msa0091930:CDS 15.0%
!! AATTACAAAAATAAAGTTCA+TGG - chr1_2:74144067-74144086 Msa0091930:intron 15.0%
!! ATTACAAAAATAAAGTTCAT+GGG - chr1_2:74144068-74144087 Msa0091930:intron 15.0%
!! TGTATAGTACATATATATAT+TGG - chr1_2:74144203-74144222 Msa0091930:CDS 15.0%
!! GTATAGTACATATATATATT+GGG - chr1_2:74144204-74144223 Msa0091930:CDS 15.0%
!! CTTTGCATAAAGAATTAAAA+AGG + chr1_2:74143299-74143318 None:intergenic 20.0%
!!! TTTTACTTCTTATTCATTCT+TGG - chr1_2:74143347-74143366 Msa0091930:three_prime_UTR 20.0%
!!! TTTTAAGAACGTAATAGTAT+TGG + chr1_2:74143745-74143764 None:intergenic 20.0%
!! TTTGTAATTAAAGAAAGAGA+TGG + chr1_2:74144056-74144075 None:intergenic 20.0%
!! AGAAAATTATTAACAAAGCT+TGG - chr1_2:74144431-74144450 Msa0091930:CDS 20.0%
!! AAGTTCTTCATTATCAAATT+TGG - chr1_2:74144681-74144700 Msa0091930:CDS 20.0%
!!! TTTTGTATAGTATATGTTGT+AGG - chr1_2:74144715-74144734 Msa0091930:CDS 20.0%
!! CTTTGCATAAAGAATTAAAA+AGG + chr1_2:74143299-74143318 None:intergenic 20.0%
!!! TTTTACTTCTTATTCATTCT+TGG - chr1_2:74143347-74143366 Msa0091930:three_prime_UTR 20.0%
!!! TTTTAAGAACGTAATAGTAT+TGG + chr1_2:74143745-74143764 None:intergenic 20.0%
!! TTTGTAATTAAAGAAAGAGA+TGG + chr1_2:74144056-74144075 None:intergenic 20.0%
!! AGAAAATTATTAACAAAGCT+TGG - chr1_2:74144431-74144450 Msa0091930:CDS 20.0%
!! AAGTTCTTCATTATCAAATT+TGG - chr1_2:74144681-74144700 Msa0091930:CDS 20.0%
!!! TTTTGTATAGTATATGTTGT+AGG - chr1_2:74144715-74144734 Msa0091930:CDS 20.0%
! AAGAATTAAAAAGGACTTCT+TGG + chr1_2:74143290-74143309 None:intergenic 25.0%
! CAAAGTACATATATTCTAGA+CGG - chr1_2:74143314-74143333 Msa0091930:three_prime_UTR 25.0%
!! ATCAAGAAGATCAATCTTTT+TGG + chr1_2:74143666-74143685 None:intergenic 25.0%
! TAACTTGAATCACTTAAACA+TGG + chr1_2:74143824-74143843 None:intergenic 25.0%
!!! TTGACTTTTTTACATGAACT+AGG + chr1_2:74143881-74143900 None:intergenic 25.0%
! ATATTTGAAAGGAATAGCAA+TGG + chr1_2:74143906-74143925 None:intergenic 25.0%
! ATATAAGGGCTATATTTGAA+AGG + chr1_2:74143917-74143936 None:intergenic 25.0%
! AGGAAATTATGCTCATTTAA+AGG + chr1_2:74144112-74144131 None:intergenic 25.0%
! ATAATTTCCTTATACTAGGT+TGG - chr1_2:74144122-74144141 Msa0091930:CDS 25.0%
! TAATTTCCTTATACTAGGTT+GGG - chr1_2:74144123-74144142 Msa0091930:CDS 25.0%
! AGTACATATATATATTGGGA+AGG - chr1_2:74144208-74144227 Msa0091930:CDS 25.0%
! ATCATTACGAAGTATATACT+AGG - chr1_2:74144262-74144281 Msa0091930:CDS 25.0%
! TGTCACACAAAGAAAATATA+TGG + chr1_2:74144895-74144914 None:intergenic 25.0%
! TAAAATACGAACACTATTTG+AGG - chr1_2:74144958-74144977 Msa0091930:five_prime_UTR 25.0%
! AAGAATTAAAAAGGACTTCT+TGG + chr1_2:74143290-74143309 None:intergenic 25.0%
! CAAAGTACATATATTCTAGA+CGG - chr1_2:74143314-74143333 Msa0091930:three_prime_UTR 25.0%
!! ATCAAGAAGATCAATCTTTT+TGG + chr1_2:74143666-74143685 None:intergenic 25.0%
! TAACTTGAATCACTTAAACA+TGG + chr1_2:74143824-74143843 None:intergenic 25.0%
!!! TTGACTTTTTTACATGAACT+AGG + chr1_2:74143881-74143900 None:intergenic 25.0%
! ATATTTGAAAGGAATAGCAA+TGG + chr1_2:74143906-74143925 None:intergenic 25.0%
! ATATAAGGGCTATATTTGAA+AGG + chr1_2:74143917-74143936 None:intergenic 25.0%
! AGGAAATTATGCTCATTTAA+AGG + chr1_2:74144112-74144131 None:intergenic 25.0%
! ATAATTTCCTTATACTAGGT+TGG - chr1_2:74144122-74144141 Msa0091930:CDS 25.0%
! TAATTTCCTTATACTAGGTT+GGG - chr1_2:74144123-74144142 Msa0091930:CDS 25.0%
! AGTACATATATATATTGGGA+AGG - chr1_2:74144208-74144227 Msa0091930:CDS 25.0%
! ATCATTACGAAGTATATACT+AGG - chr1_2:74144262-74144281 Msa0091930:CDS 25.0%
! TGTCACACAAAGAAAATATA+TGG + chr1_2:74144895-74144914 None:intergenic 25.0%
! TAAAATACGAACACTATTTG+AGG - chr1_2:74144958-74144977 Msa0091930:five_prime_UTR 25.0%
! TTTTTGCTCCTTTACAGAAT+TGG - chr1_2:74143201-74143220 Msa0091930:three_prime_UTR 30.0%
CAAAATGACCAATTCTGTAA+AGG + chr1_2:74143212-74143231 None:intergenic 30.0%
AGAAGAGTGAATTCATCATT+GGG + chr1_2:74143418-74143437 None:intergenic 30.0%
AAGAAGAGTGAATTCATCAT+TGG + chr1_2:74143419-74143438 None:intergenic 30.0%
TCACTATCCATTTCACATAA+AGG - chr1_2:74143508-74143527 Msa0091930:exon 30.0%
CACTATCCATTTCACATAAA+GGG - chr1_2:74143509-74143528 Msa0091930:exon 30.0%
! ATATGATGGAAAATGAATGG+TGG + chr1_2:74143609-74143628 None:intergenic 30.0%
! ACCATTCATTTTCCATCATA+TGG - chr1_2:74143608-74143627 Msa0091930:CDS 30.0%
! ACCATATGATGGAAAATGAA+TGG + chr1_2:74143612-74143631 None:intergenic 30.0%
ACTATCAAATCACCATATGA+TGG + chr1_2:74143623-74143642 None:intergenic 30.0%
!!! ATCAATCTTTTTGGTGTCAT+AGG + chr1_2:74143657-74143676 None:intergenic 30.0%
! AGAACGTAATAGTATTGGAA+CGG + chr1_2:74143740-74143759 None:intergenic 30.0%
!! TTTTGTTTGTGAAAGTCTTG+TGG + chr1_2:74143789-74143808 None:intergenic 30.0%
! TTTTTACATGAACTAGGCAT+AGG + chr1_2:74143875-74143894 None:intergenic 30.0%
! GACTGTTGTCTGAAATATAA+GGG + chr1_2:74143931-74143950 None:intergenic 30.0%
! AGACTGTTGTCTGAAATATA+AGG + chr1_2:74143932-74143951 None:intergenic 30.0%
TATATTTCAGACAACAGTCT+TGG - chr1_2:74143932-74143951 Msa0091930:intron 30.0%
!! TCTTGGTCTTAGATTAACAT+GGG - chr1_2:74143949-74143968 Msa0091930:intron 30.0%
AATCTGAAGAAAGCAAAGAA+TGG - chr1_2:74143981-74144000 Msa0091930:intron 30.0%
GAGCATAATTTCCTTATACT+AGG - chr1_2:74144118-74144137 Msa0091930:intron 30.0%
TTACGAAGTATATACTAGGT+TGG - chr1_2:74144266-74144285 Msa0091930:CDS 30.0%
TACGAAGTATATACTAGGTT+GGG - chr1_2:74144267-74144286 Msa0091930:CDS 30.0%
TAGTACATTATCTTTCGTGT+AGG - chr1_2:74144329-74144348 Msa0091930:CDS 30.0%
GCATAAAGGTTCATTTATCA+AGG - chr1_2:74144571-74144590 Msa0091930:CDS 30.0%
TAAAGGTTCATTTATCAAGG+AGG - chr1_2:74144574-74144593 Msa0091930:CDS 30.0%
! GATTTCTAAGGACATAGTAA+AGG - chr1_2:74144627-74144646 Msa0091930:CDS 30.0%
AGTGTCTAAACTTCAATGTT+GGG + chr1_2:74143147-74143166 None:intergenic 30.0%
AAGTGTCTAAACTTCAATGT+TGG + chr1_2:74143148-74143167 None:intergenic 30.0%
! TTTTTGCTCCTTTACAGAAT+TGG - chr1_2:74143201-74143220 Msa0091930:three_prime_UTR 30.0%
CAAAATGACCAATTCTGTAA+AGG + chr1_2:74143212-74143231 None:intergenic 30.0%
AGAAGAGTGAATTCATCATT+GGG + chr1_2:74143418-74143437 None:intergenic 30.0%
AAGAAGAGTGAATTCATCAT+TGG + chr1_2:74143419-74143438 None:intergenic 30.0%
TCACTATCCATTTCACATAA+AGG - chr1_2:74143508-74143527 Msa0091930:exon 30.0%
CACTATCCATTTCACATAAA+GGG - chr1_2:74143509-74143528 Msa0091930:exon 30.0%
! ATATGATGGAAAATGAATGG+TGG + chr1_2:74143609-74143628 None:intergenic 30.0%
! ACCATTCATTTTCCATCATA+TGG - chr1_2:74143608-74143627 Msa0091930:CDS 30.0%
! ACCATATGATGGAAAATGAA+TGG + chr1_2:74143612-74143631 None:intergenic 30.0%
ACTATCAAATCACCATATGA+TGG + chr1_2:74143623-74143642 None:intergenic 30.0%
!!! ATCAATCTTTTTGGTGTCAT+AGG + chr1_2:74143657-74143676 None:intergenic 30.0%
! AGAACGTAATAGTATTGGAA+CGG + chr1_2:74143740-74143759 None:intergenic 30.0%
!! TTTTGTTTGTGAAAGTCTTG+TGG + chr1_2:74143789-74143808 None:intergenic 30.0%
! TTTTTACATGAACTAGGCAT+AGG + chr1_2:74143875-74143894 None:intergenic 30.0%
! GACTGTTGTCTGAAATATAA+GGG + chr1_2:74143931-74143950 None:intergenic 30.0%
! AGACTGTTGTCTGAAATATA+AGG + chr1_2:74143932-74143951 None:intergenic 30.0%
TATATTTCAGACAACAGTCT+TGG - chr1_2:74143932-74143951 Msa0091930:intron 30.0%
!! TCTTGGTCTTAGATTAACAT+GGG - chr1_2:74143949-74143968 Msa0091930:intron 30.0%
AATCTGAAGAAAGCAAAGAA+TGG - chr1_2:74143981-74144000 Msa0091930:intron 30.0%
GAGCATAATTTCCTTATACT+AGG - chr1_2:74144118-74144137 Msa0091930:intron 30.0%
TTACGAAGTATATACTAGGT+TGG - chr1_2:74144266-74144285 Msa0091930:CDS 30.0%
TACGAAGTATATACTAGGTT+GGG - chr1_2:74144267-74144286 Msa0091930:CDS 30.0%
TAGTACATTATCTTTCGTGT+AGG - chr1_2:74144329-74144348 Msa0091930:CDS 30.0%
GCATAAAGGTTCATTTATCA+AGG - chr1_2:74144571-74144590 Msa0091930:CDS 30.0%
TAAAGGTTCATTTATCAAGG+AGG - chr1_2:74144574-74144593 Msa0091930:CDS 30.0%
! GATTTCTAAGGACATAGTAA+AGG - chr1_2:74144627-74144646 Msa0091930:CDS 30.0%
CAAGTAGACTTTGTGAAGTT+TGG + chr1_2:74143238-74143257 None:intergenic 35.0%
AGTGAATTCATCATTGGGAA+TGG + chr1_2:74143413-74143432 None:intergenic 35.0%
GAAGATGAAAACCAAGAAGA+GGG + chr1_2:74143447-74143466 None:intergenic 35.0%
TGAAATGGATAGTGAATGTC+AGG + chr1_2:74143503-74143522 None:intergenic 35.0%
TGTTGACCCTTTATGTGAAA+TGG + chr1_2:74143518-74143537 None:intergenic 35.0%
TATGATGATACCACTCTTTC+TGG - chr1_2:74143554-74143573 Msa0091930:CDS 35.0%
!!! CTTTTTGGTGTCATAGGAAA+TGG + chr1_2:74143651-74143670 None:intergenic 35.0%
! ATAGTATTGGAACGGTGTAA+AGG + chr1_2:74143732-74143751 None:intergenic 35.0%
!! GTCTTGGTCTTAGATTAACA+TGG - chr1_2:74143948-74143967 Msa0091930:intron 35.0%
AAACTACCCAACCTAGTATA+AGG + chr1_2:74144132-74144151 None:intergenic 35.0%
! ATATACTAGGTTGGGTAGTT+TGG - chr1_2:74144275-74144294 Msa0091930:CDS 35.0%
TCTTTCGTGTAGGATTGATT+CGG - chr1_2:74144339-74144358 Msa0091930:CDS 35.0%
CATTTATCAAGGAGGAAAGA+AGG - chr1_2:74144582-74144601 Msa0091930:CDS 35.0%
GTGTCTAAACTTCAATGTTG+GGG + chr1_2:74143146-74143165 None:intergenic 35.0%
CAAGTAGACTTTGTGAAGTT+TGG + chr1_2:74143238-74143257 None:intergenic 35.0%
AGTGAATTCATCATTGGGAA+TGG + chr1_2:74143413-74143432 None:intergenic 35.0%
GAAGATGAAAACCAAGAAGA+GGG + chr1_2:74143447-74143466 None:intergenic 35.0%
TGAAATGGATAGTGAATGTC+AGG + chr1_2:74143503-74143522 None:intergenic 35.0%
TGTTGACCCTTTATGTGAAA+TGG + chr1_2:74143518-74143537 None:intergenic 35.0%
TATGATGATACCACTCTTTC+TGG - chr1_2:74143554-74143573 Msa0091930:CDS 35.0%
!!! CTTTTTGGTGTCATAGGAAA+TGG + chr1_2:74143651-74143670 None:intergenic 35.0%
! ATAGTATTGGAACGGTGTAA+AGG + chr1_2:74143732-74143751 None:intergenic 35.0%
!! GTCTTGGTCTTAGATTAACA+TGG - chr1_2:74143948-74143967 Msa0091930:intron 35.0%
AAACTACCCAACCTAGTATA+AGG + chr1_2:74144132-74144151 None:intergenic 35.0%
! ATATACTAGGTTGGGTAGTT+TGG - chr1_2:74144275-74144294 Msa0091930:CDS 35.0%
TCTTTCGTGTAGGATTGATT+CGG - chr1_2:74144339-74144358 Msa0091930:CDS 35.0%
CATTTATCAAGGAGGAAAGA+AGG - chr1_2:74144582-74144601 Msa0091930:CDS 35.0%
GTAGACTTTGTGAAGTTTGG+TGG + chr1_2:74143235-74143254 None:intergenic 40.0%
!! TGTTGGCTTCAAAAAGCTCA+GGG + chr1_2:74143262-74143281 None:intergenic 40.0%
!! TTGTTGGCTTCAAAAAGCTC+AGG + chr1_2:74143263-74143282 None:intergenic 40.0%
AGGACTTCTTGGTTTCTTGT+TGG + chr1_2:74143279-74143298 None:intergenic 40.0%
GGAATGGAGAAGATGTTATG+AGG + chr1_2:74143397-74143416 None:intergenic 40.0%
GGAAGATGAAAACCAAGAAG+AGG + chr1_2:74143448-74143467 None:intergenic 40.0%
CAAACCTCTGTTAACTGCAA+CGG - chr1_2:74143467-74143486 Msa0091930:three_prime_UTR 40.0%
AATACCGTTGCAGTTAACAG+AGG + chr1_2:74143474-74143493 None:intergenic 40.0%
AAGCTCAAAACCAGAAAGAG+TGG + chr1_2:74143567-74143586 None:intergenic 40.0%
!!! ACTCTGTGGACACAGTTTTT+TGG + chr1_2:74143689-74143708 None:intergenic 40.0%
TAAGGTTGAGAAAGACTCTG+TGG + chr1_2:74143703-74143722 None:intergenic 40.0%
! AAAGTCTTGTGGAGCAATTG+AGG + chr1_2:74143778-74143797 None:intergenic 40.0%
AACTAGGCATAGGAACTTCA+GGG + chr1_2:74143865-74143884 None:intergenic 40.0%
TTCAACACATGTGCAGTTCT+TGG - chr1_2:74144504-74144523 Msa0091930:CDS 40.0%
! TCTCAGCATCTGTATTTTCG+TGG - chr1_2:74144527-74144546 Msa0091930:CDS 40.0%
GTCACGACTACATCAATCTA+AGG - chr1_2:74144926-74144945 Msa0091930:five_prime_UTR 40.0%
GTAGACTTTGTGAAGTTTGG+TGG + chr1_2:74143235-74143254 None:intergenic 40.0%
!! TGTTGGCTTCAAAAAGCTCA+GGG + chr1_2:74143262-74143281 None:intergenic 40.0%
!! TTGTTGGCTTCAAAAAGCTC+AGG + chr1_2:74143263-74143282 None:intergenic 40.0%
AGGACTTCTTGGTTTCTTGT+TGG + chr1_2:74143279-74143298 None:intergenic 40.0%
GGAATGGAGAAGATGTTATG+AGG + chr1_2:74143397-74143416 None:intergenic 40.0%
GGAAGATGAAAACCAAGAAG+AGG + chr1_2:74143448-74143467 None:intergenic 40.0%
CAAACCTCTGTTAACTGCAA+CGG - chr1_2:74143467-74143486 Msa0091930:three_prime_UTR 40.0%
AATACCGTTGCAGTTAACAG+AGG + chr1_2:74143474-74143493 None:intergenic 40.0%
AAGCTCAAAACCAGAAAGAG+TGG + chr1_2:74143567-74143586 None:intergenic 40.0%
!!! ACTCTGTGGACACAGTTTTT+TGG + chr1_2:74143689-74143708 None:intergenic 40.0%
TAAGGTTGAGAAAGACTCTG+TGG + chr1_2:74143703-74143722 None:intergenic 40.0%
! AAAGTCTTGTGGAGCAATTG+AGG + chr1_2:74143778-74143797 None:intergenic 40.0%
AACTAGGCATAGGAACTTCA+GGG + chr1_2:74143865-74143884 None:intergenic 40.0%
TTCAACACATGTGCAGTTCT+TGG - chr1_2:74144504-74144523 Msa0091930:CDS 40.0%
! TCTCAGCATCTGTATTTTCG+TGG - chr1_2:74144527-74144546 Msa0091930:CDS 40.0%
GTCACGACTACATCAATCTA+AGG - chr1_2:74144926-74144945 Msa0091930:five_prime_UTR 40.0%
CTTCTTTCTCACCCTCTTCT+TGG - chr1_2:74143433-74143452 Msa0091930:three_prime_UTR 45.0%
CGTTGCAGTTAACAGAGGTT+TGG + chr1_2:74143469-74143488 None:intergenic 45.0%
GTTAACTGCAACGGTATTGC+TGG - chr1_2:74143476-74143495 Msa0091930:three_prime_UTR 45.0%
ACGGTGTAAAGGTGAGGTTA+AGG + chr1_2:74143721-74143740 None:intergenic 45.0%
ATTGGAACGGTGTAAAGGTG+AGG + chr1_2:74143727-74143746 None:intergenic 45.0%
GAACTAGGCATAGGAACTTC+AGG + chr1_2:74143866-74143885 None:intergenic 45.0%
! CAGCATCTGTATTTTCGTGG+TGG - chr1_2:74144530-74144549 Msa0091930:CDS 45.0%
GGCAACACTTGTAGGCATAA+AGG - chr1_2:74144557-74144576 Msa0091930:CDS 45.0%
AGGACAATTGTTGCATAGCG+TGG - chr1_2:74144602-74144621 Msa0091930:CDS 45.0%
CATAGCGTGGCAGATTTCTA+AGG - chr1_2:74144615-74144634 Msa0091930:CDS 45.0%
!! AACATCGCTTGAAGCGTTGA+GGG + chr1_2:74144759-74144778 None:intergenic 45.0%
!! TAACATCGCTTGAAGCGTTG+AGG + chr1_2:74144760-74144779 None:intergenic 45.0%
AACGCTTCAAGCGATGTTAG+AGG - chr1_2:74144761-74144780 Msa0091930:five_prime_UTR 45.0%
CTTCTTTCTCACCCTCTTCT+TGG - chr1_2:74143433-74143452 Msa0091930:three_prime_UTR 45.0%
CGTTGCAGTTAACAGAGGTT+TGG + chr1_2:74143469-74143488 None:intergenic 45.0%
GTTAACTGCAACGGTATTGC+TGG - chr1_2:74143476-74143495 Msa0091930:three_prime_UTR 45.0%
ACGGTGTAAAGGTGAGGTTA+AGG + chr1_2:74143721-74143740 None:intergenic 45.0%
ATTGGAACGGTGTAAAGGTG+AGG + chr1_2:74143727-74143746 None:intergenic 45.0%
GAACTAGGCATAGGAACTTC+AGG + chr1_2:74143866-74143885 None:intergenic 45.0%
! CAGCATCTGTATTTTCGTGG+TGG - chr1_2:74144530-74144549 Msa0091930:CDS 45.0%
GGCAACACTTGTAGGCATAA+AGG - chr1_2:74144557-74144576 Msa0091930:CDS 45.0%
AGGACAATTGTTGCATAGCG+TGG - chr1_2:74144602-74144621 Msa0091930:CDS 45.0%
CATAGCGTGGCAGATTTCTA+AGG - chr1_2:74144615-74144634 Msa0091930:CDS 45.0%
!! AACATCGCTTGAAGCGTTGA+GGG + chr1_2:74144759-74144778 None:intergenic 45.0%
!! TAACATCGCTTGAAGCGTTG+AGG + chr1_2:74144760-74144779 None:intergenic 45.0%
AACGCTTCAAGCGATGTTAG+AGG - chr1_2:74144761-74144780 Msa0091930:five_prime_UTR 45.0%
TCTCACACAGCACGCAATAC+AGG - chr1_2:74144019-74144038 Msa0091930:intron 50.0%
! CTGTATTTTCGTGGTGGCAC+TGG - chr1_2:74144536-74144555 Msa0091930:CDS 50.0%
TCTCACACAGCACGCAATAC+AGG - chr1_2:74144019-74144038 Msa0091930:intron 50.0%
! CTGTATTTTCGTGGTGGCAC+TGG - chr1_2:74144536-74144555 Msa0091930:CDS 50.0%
!! GTGGCACTGGCAACACTTGT+AGG - chr1_2:74144549-74144568 Msa0091930:CDS 55.0%
!! GTGGCACTGGCAACACTTGT+AGG - chr1_2:74144549-74144568 Msa0091930:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 74143146 74145014 74143146 ID=Msa0091930;Name=Msa0091930
chr1_2 mRNA 74143146 74145014 74143146 ID=Msa0091930-mRNA-1;Parent=Msa0091930;Name=Msa0091930-mRNA-1;_AED=0.00;_eAED=0.00;_QI=273|1|1|1|1|1|2|379|245
chr1_2 exon 74143146 74143640 74143146 ID=Msa0091930-mRNA-1:exon:26138;Parent=Msa0091930-mRNA-1
chr1_2 exon 74144120 74145014 74144120 ID=Msa0091930-mRNA-1:exon:26137;Parent=Msa0091930-mRNA-1
chr1_2 five_prime_UTR 74144742 74145014 74144742 ID=Msa0091930-mRNA-1:five_prime_utr;Parent=Msa0091930-mRNA-1
chr1_2 CDS 74144120 74144741 74144120 ID=Msa0091930-mRNA-1:cds;Parent=Msa0091930-mRNA-1
chr1_2 CDS 74143525 74143640 74143525 ID=Msa0091930-mRNA-1:cds;Parent=Msa0091930-mRNA-1
chr1_2 three_prime_UTR 74143146 74143524 74143146 ID=Msa0091930-mRNA-1:three_prime_utr;Parent=Msa0091930-mRNA-1
Gene Sequence

>Msa0091930

ATGATGAATTCACTCTTCTTTCTCACCCTCTTCTTGGTTTTCATCTTCCAAACCTCTGTTAACTGCAACGGTATTGCTGGTCCTGACATTCACTATCCATTTCACATAAAGGGTCAACAGCAACAACAACAAGAGTATGATGATACCACTCTTTCTGGTTTTGAGCTTCTTCGCAAAGACAACATCACCACCATTCATTTTCCATCATATGGTGATTTGATAGTCAAGTCCATTTCCTATGACACCAAAAAGATTGATCTTCTTGATCCAAAAAACTGTGTCCACAGAGTCTTTCTCAACCTTAACCTCACCTTTACACCGTTCCAATACTATTACGTTCTTAAAAACTTCACATACCTCAATTGCTCCACAAGACTTTCACAAACAAAATTCATTGAAGTTCCATGTTTAAGTGATTCAAGTTATCATGTTTACACAGTTGACCCTGAAGTTCCTATGCCTAGTTCATGTAAAAAAGTCAAAACCATTGCTATTCCTTTCAAATATAGCCCTTATATTTCAGACAACAGTCTTGGTCTTAGATTAACATGGGACTTGCGTGAATCTGAAGAAAGCAAAGAATGGAACAAGACTAGAGATTCTCACACAGCACGCAATACAGTTCTTGGTCTCAGCATCTGTATTTTCGTGGTGGCACTGGCAACACTTGTAGGCATAAAGGTTCATTTATCAAGGAGGAAAGAAGGACAATTGTTGCATAGCGTGGCAGATTTCTAA
Protein sequence

>Msa0091930

MMNSLFFLTLFLVFIFQTSVNCNGIAGPDIHYPFHIKGQQQQQQEYDDTTLSGFELLRKDNITTIHFPSYGDLIVKSISYDTKKIDLLDPKNCVHRVFLNLNLTFTPFQYYYVLKNFTYLNCSTRLSQTKFIEVPCLSDSSYHVYTVDPEVPMPSSCKKVKTIAIPFKYSPYISDNSLGLRLTWDLRESEESKEWNKTRDSHTARNTVLGLSICIFVVALATLVGIKVHLSRRKEGQLLHSVADF*