Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1009270 | KEH21720.1 | 84.921 | 126 | 17 | 1 | 1 | 124 | 763 | 888 | 3.32e-62 | 214 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1009270 | sp|Q9SCZ4|FERON_ARATH | 32.117 | 137 | 74 | 3 | 1 | 124 | 765 | 895 | 3.51e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1009270 | A0A072U795 | 84.921 | 126 | 17 | 1 | 1 | 124 | 763 | 888 | 1.59e-62 | 214 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0021460 | Msa1009270 | 0.800625 | 1.320277e-48 | -8.615850e-47 |
| Msa0033340 | Msa1009270 | 0.826642 | 2.425048e-54 | -8.615850e-47 |
| Msa0109970 | Msa1009270 | 0.802153 | 6.422216e-49 | -8.615850e-47 |
| Msa0629940 | Msa1009270 | 0.843328 | 1.499616e-58 | -8.615850e-47 |
| Msa0641900 | Msa1009270 | 0.854454 | 1.218082e-61 | -8.615850e-47 |
| Msa0754180 | Msa1009270 | 0.811500 | 6.786184e-51 | -8.615850e-47 |
| Msa0811000 | Msa1009270 | 0.804637 | 1.963692e-49 | -8.615850e-47 |
| Msa0818030 | Msa1009270 | 0.806009 | 1.012745e-49 | -8.615850e-47 |
| Msa0142230 | Msa1009270 | 0.814297 | 1.654850e-51 | -8.615850e-47 |
| Msa0149170 | Msa1009270 | 0.807672 | 4.508077e-50 | -8.615850e-47 |
| Msa0156190 | Msa1009270 | 0.800229 | 1.589835e-48 | -8.615850e-47 |
| Msa0235190 | Msa1009270 | 0.800408 | 1.461687e-48 | -8.615850e-47 |
| Msa0391050 | Msa1009270 | 0.803520 | 3.351461e-49 | -8.615850e-47 |
| Msa1009270 | Msa1198090 | 0.804993 | 1.654655e-49 | -8.615850e-47 |
| Msa0584130 | Msa1009270 | 0.840184 | 1.013058e-57 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1009270 | MtrunA17_Chr7g0219721 | 84.921 | 126 | 17 | 1 | 1 | 124 | 763 | 888 | 3.28e-66 | 214 |
| Msa1009270 | MtrunA17_Chr7g0219691 | 84.127 | 126 | 18 | 1 | 1 | 124 | 763 | 888 | 4.15e-65 | 211 |
| Msa1009270 | MtrunA17_Chr7g0219671 | 69.767 | 129 | 32 | 3 | 1 | 124 | 763 | 889 | 1.12e-51 | 174 |
| Msa1009270 | MtrunA17_Chr7g0219781 | 71.318 | 129 | 30 | 3 | 1 | 124 | 766 | 892 | 2.26e-50 | 171 |
| Msa1009270 | MtrunA17_Chr7g0219761 | 77.465 | 71 | 16 | 0 | 1 | 71 | 18 | 88 | 7.26e-35 | 115 |
| Msa1009270 | MtrunA17_Chr7g0219681 | 82.540 | 63 | 11 | 0 | 1 | 63 | 754 | 816 | 2.49e-30 | 113 |
| Msa1009270 | MtrunA17_Chr7g0219581 | 44.800 | 125 | 60 | 2 | 1 | 124 | 189 | 305 | 1.28e-24 | 95.5 |
| Msa1009270 | MtrunA17_Chr7g0219571 | 44.355 | 124 | 53 | 2 | 1 | 124 | 765 | 872 | 2.17e-24 | 96.7 |
| Msa1009270 | MtrunA17_Chr4g0025961 | 44.000 | 125 | 61 | 2 | 1 | 124 | 758 | 874 | 2.37e-24 | 96.7 |
| Msa1009270 | MtrunA17_Chr7g0219651 | 44.355 | 124 | 53 | 2 | 1 | 124 | 1750 | 1857 | 8.38e-24 | 95.1 |
| Msa1009270 | MtrunA17_Chr7g0219651 | 45.161 | 124 | 58 | 3 | 1 | 124 | 754 | 867 | 7.95e-23 | 92.4 |
| Msa1009270 | MtrunA17_Chr7g0219601 | 44.800 | 125 | 60 | 2 | 1 | 124 | 749 | 865 | 3.31e-23 | 93.6 |
| Msa1009270 | MtrunA17_Chr7g0219621 | 63.492 | 63 | 23 | 0 | 1 | 63 | 156 | 218 | 5.51e-21 | 84.0 |
| Msa1009270 | MtrunA17_Chr7g0219611 | 40.000 | 125 | 52 | 3 | 1 | 124 | 745 | 847 | 3.77e-19 | 82.0 |
| Msa1009270 | MtrunA17_Chr6g0455321 | 33.824 | 136 | 78 | 2 | 1 | 124 | 765 | 900 | 6.79e-13 | 63.9 |
| Msa1009270 | MtrunA17_Chr1g0190581 | 33.600 | 125 | 74 | 2 | 1 | 124 | 752 | 868 | 8.25e-12 | 60.8 |
| Msa1009270 | MtrunA17_Chr7g0245131 | 31.159 | 138 | 73 | 4 | 1 | 124 | 764 | 893 | 7.83e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1009270 | AT3G51550.1 | 32.117 | 137 | 74 | 3 | 1 | 124 | 765 | 895 | 3.57e-12 | 62.4 |
Find 20 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAACTATCTATCGTCCTTT+TGG | 0.245350 | 7_1:+10509060 | None:intergenic |
| TAAAGGTTGTTCTTCAGTTC+TGG | 0.302035 | 7_1:+10509122 | None:intergenic |
| TTCTTCAGTTCTGGTTGAAT+TGG | 0.337677 | 7_1:+10509131 | None:intergenic |
| TTCAGTTCTGGTTGAATTGG+TGG | 0.360869 | 7_1:+10509134 | None:intergenic |
| ACAAGTAGTAGTGATGAGTA+TGG | 0.451889 | 7_1:-10509165 | Msa1009270:CDS |
| TCTGAGATTGTAAATCCAAA+AGG | 0.457133 | 7_1:-10509075 | Msa1009270:CDS |
| GATGGTGGATCCGTTCATAA+AGG | 0.467567 | 7_1:-10509421 | Msa1009270:CDS |
| TTGAGTTGTTTGCATGGTGA+TGG | 0.480383 | 7_1:-10509354 | Msa1009270:CDS |
| ATTAGAGTTTGCATTGCAGT+TGG | 0.491251 | 7_1:-10509295 | Msa1009270:CDS |
| TTTGAACTGTTATTGTCAAA+TGG | 0.492850 | 7_1:-10509379 | Msa1009270:CDS |
| TACTGATGATGGGAGTGAGA+TGG | 0.539564 | 7_1:-10509193 | Msa1009270:CDS |
| GTTATAGAATCCTTTATGAA+CGG | 0.544789 | 7_1:+10509411 | None:intergenic |
| GTAGCAGAAAACAAAGGTAA+AGG | 0.563496 | 7_1:+10509105 | None:intergenic |
| AAAAGGAAAAGAGTGAGCAA+AGG | 0.564346 | 7_1:-10509233 | Msa1009270:CDS |
| TGTCGATGAGTGATGTGGTT+GGG | 0.575459 | 7_1:-10509320 | Msa1009270:CDS |
| GCAGTTGGAGATGAGTGAGC+AGG | 0.578624 | 7_1:-10509280 | Msa1009270:CDS |
| ATGGTGTTGAGTTGTTTGCA+TGG | 0.623409 | 7_1:-10509360 | Msa1009270:CDS |
| ATGTCGATGAGTGATGTGGT+TGG | 0.684958 | 7_1:-10509321 | Msa1009270:CDS |
| ACGAATGTCGATGAGTGATG+TGG | 0.712330 | 7_1:-10509325 | Msa1009270:CDS |
| AACACCGTAGCAGAAAACAA+AGG | 0.728731 | 7_1:+10509099 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTTATAGAATCCTTTATGAA+CGG | + | chr7_1:10509080-10509099 | None:intergenic | 25.0% |
| ! | TTTGAACTGTTATTGTCAAA+TGG | - | chr7_1:10509109-10509128 | Msa1009270:CDS | 25.0% |
| !!! | TAGTAAGTTTTTTGCTACCA+AGG | - | chr7_1:10509232-10509251 | Msa1009270:CDS | 30.0% |
| ! | TTGTCACATTTTACTGATGA+TGG | - | chr7_1:10509284-10509303 | Msa1009270:CDS | 30.0% |
| ! | TGTCACATTTTACTGATGAT+GGG | - | chr7_1:10509285-10509304 | Msa1009270:CDS | 30.0% |
| !!! | TTTACCTTTGTTTTCTGCTA+CGG | - | chr7_1:10509385-10509404 | Msa1009270:CDS | 30.0% |
| ! | TCTGAGATTGTAAATCCAAA+AGG | - | chr7_1:10509413-10509432 | Msa1009270:CDS | 30.0% |
| ATTAGAGTTTGCATTGCAGT+TGG | - | chr7_1:10509193-10509212 | Msa1009270:CDS | 35.0% | |
| !!! | GTTTTTTGCTACCAAGGAAA+AGG | - | chr7_1:10509238-10509257 | Msa1009270:CDS | 35.0% |
| AAAAGGAAAAGAGTGAGCAA+AGG | - | chr7_1:10509255-10509274 | Msa1009270:CDS | 35.0% | |
| ACAAGTAGTAGTGATGAGTA+TGG | - | chr7_1:10509323-10509342 | Msa1009270:CDS | 35.0% | |
| ! | TTCTTCAGTTCTGGTTGAAT+TGG | + | chr7_1:10509360-10509379 | None:intergenic | 35.0% |
| TAAAGGTTGTTCTTCAGTTC+TGG | + | chr7_1:10509369-10509388 | None:intergenic | 35.0% | |
| GTAGCAGAAAACAAAGGTAA+AGG | + | chr7_1:10509386-10509405 | None:intergenic | 35.0% | |
| ! | ATGGTGTTGAGTTGTTTGCA+TGG | - | chr7_1:10509128-10509147 | Msa1009270:CDS | 40.0% |
| ! | TTGAGTTGTTTGCATGGTGA+TGG | - | chr7_1:10509134-10509153 | Msa1009270:CDS | 40.0% |
| ! | CTCACTCTTTTCCTTTTCCT+TGG | + | chr7_1:10509252-10509271 | None:intergenic | 40.0% |
| ! | TTCAGTTCTGGTTGAATTGG+TGG | + | chr7_1:10509357-10509376 | None:intergenic | 40.0% |
| AACACCGTAGCAGAAAACAA+AGG | + | chr7_1:10509392-10509411 | None:intergenic | 40.0% | |
| ACGAATGTCGATGAGTGATG+TGG | - | chr7_1:10509163-10509182 | Msa1009270:CDS | 45.0% | |
| ATGTCGATGAGTGATGTGGT+TGG | - | chr7_1:10509167-10509186 | Msa1009270:CDS | 45.0% | |
| TGTCGATGAGTGATGTGGTT+GGG | - | chr7_1:10509168-10509187 | Msa1009270:CDS | 45.0% | |
| !! | TACTGATGATGGGAGTGAGA+TGG | - | chr7_1:10509295-10509314 | Msa1009270:CDS | 45.0% |
| GCAGTTGGAGATGAGTGAGC+AGG | - | chr7_1:10509208-10509227 | Msa1009270:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 10509068 | 10509442 | 10509068 | ID=Msa1009270;Name=Msa1009270 |
| chr7_1 | mRNA | 10509068 | 10509442 | 10509068 | ID=Msa1009270-mRNA-1;Parent=Msa1009270;Name=Msa1009270-mRNA-1;_AED=0.58;_eAED=0.58;_QI=0|-1|0|1|-1|1|1|0|124 |
| chr7_1 | exon | 10509068 | 10509442 | 10509068 | ID=Msa1009270-mRNA-1:exon:2861;Parent=Msa1009270-mRNA-1 |
| chr7_1 | CDS | 10509068 | 10509442 | 10509068 | ID=Msa1009270-mRNA-1:cds;Parent=Msa1009270-mRNA-1 |
| Gene Sequence |
| Protein sequence |
