AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1019010


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1019010 MtrunA17_Chr7g0249081 56.000 125 50 1 19 143 138 257 1.13e-39 134
Msa1019010 MtrunA17_Chr1g0203361 75.000 76 18 1 1 76 718 792 2.43e-19 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1019010 AT1G79790.1 33.333 135 85 1 5 139 113 242 9.79e-20 82.4
Msa1019010 AT1G79790.4 41.053 95 51 1 45 139 59 148 4.96e-19 78.2
Msa1019010 AT1G79790.2 41.053 95 51 1 45 139 178 267 6.60e-18 77.8

Find 21 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATAGTTATTGTCTTCAT+AGG 0.296702 7_1:-40147083 Msa1019010:intron
CTCATTGTGTTTCAAGAAAG+AGG 0.321623 7_1:-40147461 Msa1019010:CDS
CGCGAAGAACTGTCATTGTT+GGG 0.354663 7_1:-40146991 Msa1019010:CDS
TCCTCATAGAATTCAGTATC+CGG 0.447494 7_1:+40147435 None:intergenic
GCGCGAAGAACTGTCATTGT+TGG 0.454550 7_1:-40146992 Msa1019010:CDS
GTGGAGGCAGCAGCTGAAGT+TGG 0.459723 7_1:-40147051 Msa1019010:CDS
AGCAGCTGAAGTTGGGATCA+AGG 0.474632 7_1:-40147043 Msa1019010:CDS
TGGAGGCAGCAGCTGAAGTT+GGG 0.474813 7_1:-40147050 Msa1019010:CDS
AGAAGCAGAATTAAATGCTA+TGG 0.479810 7_1:-40148210 Msa1019010:CDS
GTCAAAATCAGAAATGCATA+AGG 0.483727 7_1:-40148105 Msa1019010:CDS
AGCTGCCTCATTGTCTCTTG+AGG 0.501894 7_1:+40148145 None:intergenic
TGTTTCAAGAAAGAGGAAAC+CGG 0.511148 7_1:-40147454 Msa1019010:CDS
AATGCATAAGGTAATTGCTC+AGG 0.523450 7_1:-40148093 Msa1019010:intron
TATATCTCAATTAGAAGAAG+AGG 0.523544 7_1:-40148243 Msa1019010:CDS
AAGAGACAATGAGGCAGCTA+AGG 0.534926 7_1:-40148141 Msa1019010:CDS
ACCGGATACTGAATTCTATG+AGG 0.572307 7_1:-40147436 Msa1019010:CDS
CTCAAGAATAACAAAGGACG+AGG 0.579104 7_1:-40148179 Msa1019010:CDS
GCGAAGAACTGTCATTGTTG+GGG 0.610758 7_1:-40146990 Msa1019010:CDS
AAGAAGCTCAAGAATAACAA+AGG 0.616117 7_1:-40148185 Msa1019010:CDS
TATGACCTCAAGAGACAATG+AGG 0.680939 7_1:-40148150 Msa1019010:CDS
AATTCTATGAGGAAGTTGTG+AGG 0.684361 7_1:-40147425 Msa1019010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTATAAGTGATATCA+TGG + chr7_1:40147902-40147921 None:intergenic 15.0%
!! ATAAATTATAAGTGATATCA+TGG + chr7_1:40147902-40147921 None:intergenic 15.0%
!! TTGATATCTGATAATAAGTA+TGG + chr7_1:40147151-40147170 None:intergenic 20.0%
!!! CTCAAAGTTATTTTTCTTTA+AGG + chr7_1:40147390-40147409 None:intergenic 20.0%
!! TGATATTATCTACATTCTAA+TGG - chr7_1:40147648-40147667 Msa1019010:intron 20.0%
!! CAAAAAAACTTATCATTTGA+GGG - chr7_1:40147673-40147692 Msa1019010:intron 20.0%
!! GAATGTATCTACAATATATA+AGG + chr7_1:40147734-40147753 None:intergenic 20.0%
!!! CTTTGCTTCTATTTTTTAAT+AGG + chr7_1:40148040-40148059 None:intergenic 20.0%
!! TATATAGTTATTGTCTTCAT+AGG - chr7_1:40148131-40148150 Msa1019010:CDS 20.0%
!! TTGATATCTGATAATAAGTA+TGG + chr7_1:40147151-40147170 None:intergenic 20.0%
!!! CTCAAAGTTATTTTTCTTTA+AGG + chr7_1:40147390-40147409 None:intergenic 20.0%
!! TGATATTATCTACATTCTAA+TGG - chr7_1:40147648-40147667 Msa1019010:intron 20.0%
!! CAAAAAAACTTATCATTTGA+GGG - chr7_1:40147673-40147692 Msa1019010:intron 20.0%
!! GAATGTATCTACAATATATA+AGG + chr7_1:40147734-40147753 None:intergenic 20.0%
!!! CTTTGCTTCTATTTTTTAAT+AGG + chr7_1:40148040-40148059 None:intergenic 20.0%
!! TATATAGTTATTGTCTTCAT+AGG - chr7_1:40148131-40148150 Msa1019010:CDS 20.0%
! TATATCTCAATTAGAAGAAG+AGG - chr7_1:40146971-40146990 Msa1019010:CDS 25.0%
! GAACATACTTATTGCATATT+AGG - chr7_1:40147171-40147190 Msa1019010:intron 25.0%
! ACATTGATGAGTTTATTATG+TGG - chr7_1:40147214-40147233 Msa1019010:intron 25.0%
! TTAAAACTTGTATGAGACAT+GGG - chr7_1:40147327-40147346 Msa1019010:intron 25.0%
!! CTTTTACTAACTATCCTATA+TGG - chr7_1:40147417-40147436 Msa1019010:CDS 25.0%
!!! CCTCAAATGATAAGTTTTTT+TGG + chr7_1:40147675-40147694 None:intergenic 25.0%
! CCAAAAAAACTTATCATTTG+AGG - chr7_1:40147672-40147691 Msa1019010:intron 25.0%
!! ATTGTATTTTCATAGATGAC+AGG - chr7_1:40147834-40147853 Msa1019010:intron 25.0%
!! TTTTAATAGGCAAATGTTAG+TGG + chr7_1:40148027-40148046 None:intergenic 25.0%
! GAAGCAAAGTTCAATTTATT+TGG - chr7_1:40148051-40148070 Msa1019010:intron 25.0%
! TATATCTCAATTAGAAGAAG+AGG - chr7_1:40146971-40146990 Msa1019010:CDS 25.0%
! GAACATACTTATTGCATATT+AGG - chr7_1:40147171-40147190 Msa1019010:intron 25.0%
! ACATTGATGAGTTTATTATG+TGG - chr7_1:40147214-40147233 Msa1019010:intron 25.0%
! TTAAAACTTGTATGAGACAT+GGG - chr7_1:40147327-40147346 Msa1019010:intron 25.0%
!! CTTTTACTAACTATCCTATA+TGG - chr7_1:40147417-40147436 Msa1019010:CDS 25.0%
!!! CCTCAAATGATAAGTTTTTT+TGG + chr7_1:40147675-40147694 None:intergenic 25.0%
! CCAAAAAAACTTATCATTTG+AGG - chr7_1:40147672-40147691 Msa1019010:intron 25.0%
!! ATTGTATTTTCATAGATGAC+AGG - chr7_1:40147834-40147853 Msa1019010:intron 25.0%
!! TTTTAATAGGCAAATGTTAG+TGG + chr7_1:40148027-40148046 None:intergenic 25.0%
! GAAGCAAAGTTCAATTTATT+TGG - chr7_1:40148051-40148070 Msa1019010:intron 25.0%
AGAAGCAGAATTAAATGCTA+TGG - chr7_1:40147004-40147023 Msa1019010:CDS 30.0%
AAGAAGCTCAAGAATAACAA+AGG - chr7_1:40147029-40147048 Msa1019010:CDS 30.0%
GTCAAAATCAGAAATGCATA+AGG - chr7_1:40147109-40147128 Msa1019010:intron 30.0%
TACTTATTGCATATTAGGAC+AGG - chr7_1:40147176-40147195 Msa1019010:intron 30.0%
ACATATGATCTTTAACTGTC+AGG - chr7_1:40147261-40147280 Msa1019010:intron 30.0%
! TGTTCTTCTTTTTCTTTGAC+AGG - chr7_1:40147302-40147321 Msa1019010:intron 30.0%
! CATGTCTCATACAAGTTTTA+AGG + chr7_1:40147328-40147347 None:intergenic 30.0%
CTTAAAACTTGTATGAGACA+TGG - chr7_1:40147326-40147345 Msa1019010:intron 30.0%
TATCCTATATGGTAAGACAA+TGG - chr7_1:40147428-40147447 Msa1019010:CDS 30.0%
AATCCATTGTCTTACCATAT+AGG + chr7_1:40147434-40147453 None:intergenic 30.0%
TTGAAATTGTAAGTACCAGT+TGG - chr7_1:40147507-40147526 Msa1019010:intron 30.0%
AGATTTGTATGCAATACTGA+AGG - chr7_1:40147938-40147957 Msa1019010:intron 30.0%
AGAAGCAGAATTAAATGCTA+TGG - chr7_1:40147004-40147023 Msa1019010:CDS 30.0%
AAGAAGCTCAAGAATAACAA+AGG - chr7_1:40147029-40147048 Msa1019010:CDS 30.0%
GTCAAAATCAGAAATGCATA+AGG - chr7_1:40147109-40147128 Msa1019010:intron 30.0%
TACTTATTGCATATTAGGAC+AGG - chr7_1:40147176-40147195 Msa1019010:intron 30.0%
ACATATGATCTTTAACTGTC+AGG - chr7_1:40147261-40147280 Msa1019010:intron 30.0%
! TGTTCTTCTTTTTCTTTGAC+AGG - chr7_1:40147302-40147321 Msa1019010:intron 30.0%
! CATGTCTCATACAAGTTTTA+AGG + chr7_1:40147328-40147347 None:intergenic 30.0%
CTTAAAACTTGTATGAGACA+TGG - chr7_1:40147326-40147345 Msa1019010:intron 30.0%
TATCCTATATGGTAAGACAA+TGG - chr7_1:40147428-40147447 Msa1019010:CDS 30.0%
AATCCATTGTCTTACCATAT+AGG + chr7_1:40147434-40147453 None:intergenic 30.0%
TTGAAATTGTAAGTACCAGT+TGG - chr7_1:40147507-40147526 Msa1019010:intron 30.0%
AGATTTGTATGCAATACTGA+AGG - chr7_1:40147938-40147957 Msa1019010:intron 30.0%
AATGCATAAGGTAATTGCTC+AGG - chr7_1:40147121-40147140 Msa1019010:intron 35.0%
GCATATTAGGACAGGTTAAA+TGG - chr7_1:40147184-40147203 Msa1019010:intron 35.0%
CATGGGTATTCATACATAGA+AGG - chr7_1:40147344-40147363 Msa1019010:intron 35.0%
CAAAAAGATGATCGACACAA+AGG + chr7_1:40147620-40147639 None:intergenic 35.0%
!! TGTGTCGATCATCTTTTTGT+TGG - chr7_1:40147621-40147640 Msa1019010:intron 35.0%
CTCATTGTGTTTCAAGAAAG+AGG - chr7_1:40147753-40147772 Msa1019010:intron 35.0%
TGTTTCAAGAAAGAGGAAAC+CGG - chr7_1:40147760-40147779 Msa1019010:intron 35.0%
TCCTCATAGAATTCAGTATC+CGG + chr7_1:40147782-40147801 None:intergenic 35.0%
AATTCTATGAGGAAGTTGTG+AGG - chr7_1:40147789-40147808 Msa1019010:intron 35.0%
!! GCAATACTGAAGGAGTATTA+AGG - chr7_1:40147948-40147967 Msa1019010:intron 35.0%
!! ATACTGAAGGAGTATTAAGG+AGG - chr7_1:40147951-40147970 Msa1019010:intron 35.0%
!! AAGGAGTATTAAGGAGGTTT+AGG - chr7_1:40147957-40147976 Msa1019010:intron 35.0%
!! AGGAGTATTAAGGAGGTTTA+GGG - chr7_1:40147958-40147977 Msa1019010:intron 35.0%
!! TGTTAGTGGATGGTTTGTTA+GGG + chr7_1:40148013-40148032 None:intergenic 35.0%
!! ATGTTAGTGGATGGTTTGTT+AGG + chr7_1:40148014-40148033 None:intergenic 35.0%
AATAGGCAAATGTTAGTGGA+TGG + chr7_1:40148023-40148042 None:intergenic 35.0%
CTACTTCAACTTCAACTGTT+TGG + chr7_1:40148089-40148108 None:intergenic 35.0%
TCTTCATAGGCCAAAAAATG+TGG - chr7_1:40148144-40148163 Msa1019010:CDS 35.0%
AATGCATAAGGTAATTGCTC+AGG - chr7_1:40147121-40147140 Msa1019010:intron 35.0%
GCATATTAGGACAGGTTAAA+TGG - chr7_1:40147184-40147203 Msa1019010:intron 35.0%
CATGGGTATTCATACATAGA+AGG - chr7_1:40147344-40147363 Msa1019010:intron 35.0%
CAAAAAGATGATCGACACAA+AGG + chr7_1:40147620-40147639 None:intergenic 35.0%
!! TGTGTCGATCATCTTTTTGT+TGG - chr7_1:40147621-40147640 Msa1019010:intron 35.0%
CTCATTGTGTTTCAAGAAAG+AGG - chr7_1:40147753-40147772 Msa1019010:intron 35.0%
TGTTTCAAGAAAGAGGAAAC+CGG - chr7_1:40147760-40147779 Msa1019010:intron 35.0%
TCCTCATAGAATTCAGTATC+CGG + chr7_1:40147782-40147801 None:intergenic 35.0%
AATTCTATGAGGAAGTTGTG+AGG - chr7_1:40147789-40147808 Msa1019010:intron 35.0%
!! GCAATACTGAAGGAGTATTA+AGG - chr7_1:40147948-40147967 Msa1019010:intron 35.0%
!! ATACTGAAGGAGTATTAAGG+AGG - chr7_1:40147951-40147970 Msa1019010:intron 35.0%
!! AAGGAGTATTAAGGAGGTTT+AGG - chr7_1:40147957-40147976 Msa1019010:intron 35.0%
!! AGGAGTATTAAGGAGGTTTA+GGG - chr7_1:40147958-40147977 Msa1019010:intron 35.0%
!! TGTTAGTGGATGGTTTGTTA+GGG + chr7_1:40148013-40148032 None:intergenic 35.0%
!! ATGTTAGTGGATGGTTTGTT+AGG + chr7_1:40148014-40148033 None:intergenic 35.0%
AATAGGCAAATGTTAGTGGA+TGG + chr7_1:40148023-40148042 None:intergenic 35.0%
CTACTTCAACTTCAACTGTT+TGG + chr7_1:40148089-40148108 None:intergenic 35.0%
TCTTCATAGGCCAAAAAATG+TGG - chr7_1:40148144-40148163 Msa1019010:CDS 35.0%
CTCAAGAATAACAAAGGACG+AGG - chr7_1:40147035-40147054 Msa1019010:CDS 40.0%
TATGACCTCAAGAGACAATG+AGG - chr7_1:40147064-40147083 Msa1019010:CDS 40.0%
ACCGGATACTGAATTCTATG+AGG - chr7_1:40147778-40147797 Msa1019010:intron 40.0%
!! GTTAGTGGATGGTTTGTTAG+GGG + chr7_1:40148012-40148031 None:intergenic 40.0%
TCATAGGCCAAAAAATGTGG+AGG - chr7_1:40148147-40148166 Msa1019010:CDS 40.0%
CTCAAGAATAACAAAGGACG+AGG - chr7_1:40147035-40147054 Msa1019010:CDS 40.0%
TATGACCTCAAGAGACAATG+AGG - chr7_1:40147064-40147083 Msa1019010:CDS 40.0%
ACCGGATACTGAATTCTATG+AGG - chr7_1:40147778-40147797 Msa1019010:intron 40.0%
!! GTTAGTGGATGGTTTGTTAG+GGG + chr7_1:40148012-40148031 None:intergenic 40.0%
TCATAGGCCAAAAAATGTGG+AGG - chr7_1:40148147-40148166 Msa1019010:CDS 40.0%
AAGAGACAATGAGGCAGCTA+AGG - chr7_1:40147073-40147092 Msa1019010:CDS 45.0%
TTGTAAGTACCAGTTGGTCG+AGG - chr7_1:40147513-40147532 Msa1019010:intron 45.0%
!! TTAACTTGTCCTCGACCAAC+TGG + chr7_1:40147525-40147544 None:intergenic 45.0%
!! ACGTTTTGTTCGTGTGCACT+TGG - chr7_1:40147568-40147587 Msa1019010:intron 45.0%
!! TGGATGGTTTGTTAGGGGTA+GGG + chr7_1:40148007-40148026 None:intergenic 45.0%
CGCGAAGAACTGTCATTGTT+GGG - chr7_1:40148223-40148242 Msa1019010:CDS 45.0%
GCGAAGAACTGTCATTGTTG+GGG - chr7_1:40148224-40148243 Msa1019010:CDS 45.0%
AAGAGACAATGAGGCAGCTA+AGG - chr7_1:40147073-40147092 Msa1019010:CDS 45.0%
TTGTAAGTACCAGTTGGTCG+AGG - chr7_1:40147513-40147532 Msa1019010:intron 45.0%
!! TTAACTTGTCCTCGACCAAC+TGG + chr7_1:40147525-40147544 None:intergenic 45.0%
!! ACGTTTTGTTCGTGTGCACT+TGG - chr7_1:40147568-40147587 Msa1019010:intron 45.0%
!! TGGATGGTTTGTTAGGGGTA+GGG + chr7_1:40148007-40148026 None:intergenic 45.0%
CGCGAAGAACTGTCATTGTT+GGG - chr7_1:40148223-40148242 Msa1019010:CDS 45.0%
GCGAAGAACTGTCATTGTTG+GGG - chr7_1:40148224-40148243 Msa1019010:CDS 45.0%
AGCTGCCTCATTGTCTCTTG+AGG + chr7_1:40147072-40147091 None:intergenic 50.0%
! ACACAAAGGCGCAGAGAGAA+AGG + chr7_1:40147606-40147625 None:intergenic 50.0%
!! GTGGATGGTTTGTTAGGGGT+AGG + chr7_1:40148008-40148027 None:intergenic 50.0%
!!! GCTGCTGCCTCCACATTTTT+TGG + chr7_1:40148157-40148176 None:intergenic 50.0%
AGCAGCTGAAGTTGGGATCA+AGG - chr7_1:40148171-40148190 Msa1019010:CDS 50.0%
GCGCGAAGAACTGTCATTGT+TGG - chr7_1:40148222-40148241 Msa1019010:CDS 50.0%
AGCTGCCTCATTGTCTCTTG+AGG + chr7_1:40147072-40147091 None:intergenic 50.0%
! ACACAAAGGCGCAGAGAGAA+AGG + chr7_1:40147606-40147625 None:intergenic 50.0%
!! GTGGATGGTTTGTTAGGGGT+AGG + chr7_1:40148008-40148027 None:intergenic 50.0%
!!! GCTGCTGCCTCCACATTTTT+TGG + chr7_1:40148157-40148176 None:intergenic 50.0%
AGCAGCTGAAGTTGGGATCA+AGG - chr7_1:40148171-40148190 Msa1019010:CDS 50.0%
GCGCGAAGAACTGTCATTGT+TGG - chr7_1:40148222-40148241 Msa1019010:CDS 50.0%
TGGAGGCAGCAGCTGAAGTT+GGG - chr7_1:40148164-40148183 Msa1019010:CDS 55.0%
TGGAGGCAGCAGCTGAAGTT+GGG - chr7_1:40148164-40148183 Msa1019010:CDS 55.0%
GTGGAGGCAGCAGCTGAAGT+TGG - chr7_1:40148163-40148182 Msa1019010:CDS 60.0%
GTGGAGGCAGCAGCTGAAGT+TGG - chr7_1:40148163-40148182 Msa1019010:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 40146960 40148276 40146960 ID=Msa1019010;Name=Msa1019010
chr7_1 mRNA 40146960 40148276 40146960 ID=Msa1019010-mRNA-1;Parent=Msa1019010;Name=Msa1019010-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|0.66|1|1|3|0|143
chr7_1 exon 40148094 40148276 40148094 ID=Msa1019010-mRNA-1:exon:7763;Parent=Msa1019010-mRNA-1
chr7_1 exon 40147381 40147490 40147381 ID=Msa1019010-mRNA-1:exon:7762;Parent=Msa1019010-mRNA-1
chr7_1 exon 40146960 40147098 40146960 ID=Msa1019010-mRNA-1:exon:7761;Parent=Msa1019010-mRNA-1
chr7_1 CDS 40148094 40148276 40148094 ID=Msa1019010-mRNA-1:cds;Parent=Msa1019010-mRNA-1
chr7_1 CDS 40147381 40147490 40147381 ID=Msa1019010-mRNA-1:cds;Parent=Msa1019010-mRNA-1
chr7_1 CDS 40146960 40147098 40146960 ID=Msa1019010-mRNA-1:cds;Parent=Msa1019010-mRNA-1
Gene Sequence

>Msa1019010

ATGAAGAAACATATATCTCAATTAGAAGAAGAGGTCAAGAAGAAAGAAGCAGAATTAAATGCTATGGAAAAGAAGCTCAAGAATAACAAAGGACGAGGTGCAGCTATGACCTCAAGAGACAATGAGGCAGCTAAGGCAAATGCGAAGAAGTCAAAATCAGAAATGCATAAGGTAATTGCTCAGATACATTCTCATTGTGTTTCAAGAAAGAGGAAACCGGATACTGAATTCTATGAGGAAGTTGTGAGGCATCTTGAAGTTGATCTAACATATTGTATTTTCATAGATGACAGTTATTGTCTTCATAGGCCAAAAAATGTGGAGGCAGCAGCTGAAGTTGGGATCAAGGATGTACATTTTAAGAATGTCGATTTAATGCGCGAAGAACTGTCATTGTTGGGGATTGACATTTCAACAGATGAAGATCAATAG

Protein sequence

>Msa1019010

MKKHISQLEEEVKKKEAELNAMEKKLKNNKGRGAAMTSRDNEAAKANAKKSKSEMHKVIAQIHSHCVSRKRKPDTEFYEEVVRHLEVDLTYCIFIDDSYCLHRPKNVEAAAEVGIKDVHFKNVDLMREELSLLGIDISTDEDQ*