AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0676760


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0676760 MtrunA17_Chr8g0388331 91.935 62 5 0 1 62 132 193 8.34e-35 116
Msa0676760 MtrunA17_Chr8g0346911 67.213 61 20 0 1 61 155 215 2.72e-24 90.1
Msa0676760 MtrunA17_Chr3g0126801 56.452 62 27 0 1 62 26 87 9.59e-22 80.9
Msa0676760 MtrunA17_Chr5g0399931 54.839 62 28 0 1 62 1 62 8.92e-21 77.4
Msa0676760 MtrunA17_Chr1g0151621 58.065 62 26 0 1 62 171 232 1.86e-20 80.5
Msa0676760 MtrunA17_Chr4g0055421 53.226 62 29 0 1 62 132 193 2.90e-20 79.3
Msa0676760 MtrunA17_Chr4g0076551 61.290 62 24 0 1 62 148 209 9.16e-19 75.9
Msa0676760 MtrunA17_Chr8g0388311 51.613 62 30 0 1 62 1 62 2.13e-18 71.2
Msa0676760 MtrunA17_Chr3g0102821 59.677 62 25 0 1 62 147 208 3.28e-18 74.3
Msa0676760 MtrunA17_Chr2g0290771 55.738 61 27 0 1 61 112 172 6.06e-18 72.8
Msa0676760 MtrunA17_Chr5g0446931 58.065 62 26 0 1 62 147 208 7.24e-18 73.2
Msa0676760 MtrunA17_Chr2g0290751 57.377 61 26 0 1 61 150 210 3.65e-17 71.2
Msa0676760 MtrunA17_Chr2g0290761 55.738 61 27 0 1 61 150 210 2.64e-16 68.9
Msa0676760 MtrunA17_Chr4g0063121 40.323 62 37 0 1 62 89 150 1.36e-13 62.0
Msa0676760 MtrunA17_Chr2g0290781 50.820 61 30 0 1 61 152 212 3.68e-12 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0676760 AT1G64480.4 61.290 62 24 0 1 62 136 197 3.95e-19 76.6
Msa0676760 AT1G64480.2 61.290 62 24 0 1 62 136 197 6.05e-19 76.3
Msa0676760 AT1G64480.3 61.290 62 24 0 1 62 136 197 6.05e-19 76.3
Msa0676760 AT1G64480.1 61.290 62 24 0 1 62 136 197 6.05e-19 76.3
Msa0676760 AT5G24270.3 53.226 62 29 0 1 62 89 150 9.44e-19 75.1
Msa0676760 AT4G17615.2 53.226 62 29 0 1 62 90 151 1.89e-18 73.9
Msa0676760 AT5G24270.5 53.226 62 29 0 1 62 111 172 2.14e-18 74.7
Msa0676760 AT5G47100.1 53.226 62 29 0 1 62 132 193 2.87e-18 74.7
Msa0676760 AT5G24270.4 53.226 62 29 0 1 62 136 197 4.43e-18 74.3
Msa0676760 AT5G24270.1 53.226 62 29 0 1 62 136 197 4.43e-18 74.3
Msa0676760 AT5G24270.2 53.226 62 29 0 1 62 136 197 4.43e-18 74.3
Msa0676760 AT4G17615.4 53.226 62 29 0 1 62 132 193 4.93e-18 73.9
Msa0676760 AT4G17615.1 53.226 62 29 0 1 62 132 193 4.93e-18 73.9
Msa0676760 AT4G17615.5 53.226 62 29 0 1 62 132 193 4.93e-18 73.9
Msa0676760 AT4G26570.2 58.065 62 26 0 1 62 151 212 7.82e-18 73.6
Msa0676760 AT4G26570.1 58.065 62 26 0 1 62 147 208 9.00e-18 73.6
Msa0676760 AT4G17615.3 53.226 62 29 0 1 62 132 193 8.79e-17 71.2
Msa0676760 AT5G55990.1 54.839 62 28 0 1 62 147 208 3.11e-16 69.3
Msa0676760 AT5G55990.2 54.839 62 28 0 1 62 147 208 3.11e-16 69.3
Msa0676760 AT4G33000.1 53.226 62 29 0 1 62 178 239 2.72e-15 67.4
Msa0676760 AT4G33000.2 53.226 62 29 0 1 62 168 229 3.78e-15 67.0
Msa0676760 AT4G16350.2 51.613 62 30 0 1 62 141 202 8.30e-15 65.9
Msa0676760 AT4G16350.1 51.613 62 30 0 1 62 141 202 8.30e-15 65.9
Msa0676760 AT4G01420.2 48.387 62 32 0 1 62 131 192 8.18e-14 62.4
Msa0676760 AT4G01420.1 48.387 62 32 0 1 62 131 192 9.29e-14 62.4
Msa0676760 AT4G26560.1 51.613 62 30 0 1 62 135 196 2.79e-13 61.6

Find 14 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATATTGTTGAATCCATTA+TGG 0.253676 4_4:-28008835 Msa0676760:CDS
TGTTGAATCCATTATGGAAA+AGG 0.404316 4_4:-28008829 Msa0676760:intron
AGGAGATGGGAAGATTGATA+TGG 0.412059 4_4:-28008679 Msa0676760:CDS
AAAGAAACCTACTTTAGATA+TGG 0.414269 4_4:+28008600 None:intergenic
ATGAAGGAGGTTGACTCAAA+AGG 0.481217 4_4:-28008699 Msa0676760:CDS
GAAGTTGAAGGAGATGGTCT+TGG 0.495842 4_4:-28008892 Msa0676760:exon
GTCATGATCTTAAGAAGAGA+AGG 0.521718 4_4:+28008627 None:intergenic
GAGGTTGACTCAAAAGGAGA+TGG 0.527295 4_4:-28008693 Msa0676760:CDS
TAATGGCATACTGAAGTTGA+AGG 0.542035 4_4:-28008904 Msa0676760:five_prime_UTR
ACTTTAGTAGTTATAGACTA+CGG 0.573937 4_4:-28008984 Msa0676760:five_prime_UTR
GACTCTGCCATATCTAAAGT+AGG 0.577386 4_4:-28008607 None:intergenic
GGAAGATTGATATGGAAGAG+TGG 0.580086 4_4:-28008671 Msa0676760:CDS
CATACTGAAGTTGAAGGAGA+TGG 0.587037 4_4:-28008898 Msa0676760:exon
AGGTTGACTCAAAAGGAGAT+GGG 0.621613 4_4:-28008692 Msa0676760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACTTTAGTAGTTATAGACTA+CGG - chr4_4:28008610-28008629 Msa0676760:CDS 25.0%
!!! TGAACCTTTTTTGTACATAA+TGG - chr4_4:28008673-28008692 Msa0676760:CDS 25.0%
! TATGCCATTATGTACAAAAA+AGG + chr4_4:28008680-28008699 None:intergenic 25.0%
! GATAAATGTATGGTTTCATA+TGG + chr4_4:28008798-28008817 None:intergenic 25.0%
! AAGAGAAAAGAATGAAAATG+AGG + chr4_4:28008842-28008861 None:intergenic 25.0%
! TTTCTCTTATACTTCTTTCT+TGG - chr4_4:28008854-28008873 Msa0676760:CDS 25.0%
! TTCATTGTCTAAAAAAGATC+AGG + chr4_4:28008880-28008899 None:intergenic 25.0%
!!! TGATCTTTTTTAGACAATGA+AGG - chr4_4:28008879-28008898 Msa0676760:CDS 25.0%
CGATATTGTTGAATCCATTA+TGG - chr4_4:28008759-28008778 Msa0676760:intron 30.0%
TGTTGAATCCATTATGGAAA+AGG - chr4_4:28008765-28008784 Msa0676760:intron 30.0%
! GTTAAGAACCTTTTCCATAA+TGG + chr4_4:28008776-28008795 None:intergenic 30.0%
ATTGAAGGAGGATAAATGTA+TGG + chr4_4:28008808-28008827 None:intergenic 30.0%
GAAACTATAGAAATTGAAGG+AGG + chr4_4:28008820-28008839 None:intergenic 30.0%
GAGGAAACTATAGAAATTGA+AGG + chr4_4:28008823-28008842 None:intergenic 30.0%
!!! TCTTTTTTAGACAATGAAGG+AGG - chr4_4:28008882-28008901 Msa0676760:CDS 30.0%
TAATGGCATACTGAAGTTGA+AGG - chr4_4:28008690-28008709 Msa0676760:CDS 35.0%
GTCATGATCTTAAGAAGAGA+AGG + chr4_4:28008970-28008989 None:intergenic 35.0%
CATACTGAAGTTGAAGGAGA+TGG - chr4_4:28008696-28008715 Msa0676760:CDS 40.0%
! ATGAAGGAGGTTGACTCAAA+AGG - chr4_4:28008895-28008914 Msa0676760:exon 40.0%
! AGGTTGACTCAAAAGGAGAT+GGG - chr4_4:28008902-28008921 Msa0676760:five_prime_UTR 40.0%
AGGAGATGGGAAGATTGATA+TGG - chr4_4:28008915-28008934 Msa0676760:five_prime_UTR 40.0%
GGAAGATTGATATGGAAGAG+TGG - chr4_4:28008923-28008942 Msa0676760:five_prime_UTR 40.0%
GAAGTTGAAGGAGATGGTCT+TGG - chr4_4:28008702-28008721 Msa0676760:CDS 45.0%
! GAGGTTGACTCAAAAGGAGA+TGG - chr4_4:28008901-28008920 Msa0676760:exon 45.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 28008608 28009008 28008608 ID=Msa0676760;Name=Msa0676760
chr4_4 mRNA 28008608 28009008 28008608 ID=Msa0676760-mRNA-1;Parent=Msa0676760;Name=Msa0676760-mRNA-1;_AED=0.08;_eAED=0.08;_QI=107|1|0.5|1|1|1|2|0|62
chr4_4 exon 28008608 28008724 28008608 ID=Msa0676760-mRNA-1:exon:7379;Parent=Msa0676760-mRNA-1
chr4_4 exon 28008830 28009008 28008830 ID=Msa0676760-mRNA-1:exon:7378;Parent=Msa0676760-mRNA-1
chr4_4 five_prime_UTR 28008902 28009008 28008902 ID=Msa0676760-mRNA-1:five_prime_utr;Parent=Msa0676760-mRNA-1
chr4_4 CDS 28008830 28008901 28008830 ID=Msa0676760-mRNA-1:cds;Parent=Msa0676760-mRNA-1
chr4_4 CDS 28008608 28008724 28008608 ID=Msa0676760-mRNA-1:cds;Parent=Msa0676760-mRNA-1
Gene Sequence

>Msa0676760

ATGGTCTTGGCTACTTTGATTGAATCAGAAGTTACAGTTTCAGACGATATTGTTGAATCCATTATGGAAAAGACAATGAAGGAGGTTGACTCAAAAGGAGATGGGAAGATTGATATGGAAGAGTGGAAAGAGTATGCAGCAAAGAATCCTTCTCTTCTTAAGATCATGACTCTGCCATATCTAAAGTAG

Protein sequence

>Msa0676760

MVLATLIESEVTVSDDIVESIMEKTMKEVDSKGDGKIDMEEWKEYAAKNPSLLKIMTLPYLK*