AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1139460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1139460 MtrunA17_Chr7g0218691 92.086 417 20 4 26 435 25 435 0.0 707
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1139460 AT3G29310.1 31.236 445 220 17 9 390 15 436 2.05e-37 143

Find 131 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAAATTGTGTAAGGTTTA+TGG 0.131706 7_4:+9887230 Msa1139460:CDS
TTGGCATTTGGTTCCATAAA+TGG 0.167649 7_4:+9887874 Msa1139460:three_prime_UTR
GTAATGTTTGATTGTATTCT+AGG 0.171441 7_4:+9887794 Msa1139460:three_prime_UTR
TGATAGATGCAAGTTGTTTA+AGG 0.193783 7_4:-9886125 None:intergenic
TGCTCCATTGCCACTTAAAA+TGG 0.217047 7_4:+9887676 Msa1139460:CDS
TGATGAAGGGTTTGTGATTT+GGG 0.218801 7_4:-9885931 None:intergenic
AACTTAGAATGTTTAGTAAT+TGG 0.244051 7_4:+9887829 Msa1139460:three_prime_UTR
TGGGTTGGAGGAGGTGGTGG+TGG 0.257478 7_4:-9885742 None:intergenic
GAACATAACAAATCCATTTA+TGG 0.260273 7_4:-9887887 None:intergenic
TTCTCATCATCATCACCTTC+TGG 0.268545 7_4:-9887164 None:intergenic
GTTGGAGGAGGTGGTGGTGG+TGG 0.273057 7_4:-9885739 None:intergenic
GATGGGGTGGTTGTGGTGTG+GGG 0.278446 7_4:-9885798 None:intergenic
GGAGGAGGTGGTGGTGGTGG+AGG 0.285241 7_4:-9885736 None:intergenic
CATCAAACTTGATTCAATTC+AGG 0.291591 7_4:+9886236 Msa1139460:CDS
CTGTGTGTCTAATTGGCATT+TGG 0.295953 7_4:+9887862 Msa1139460:three_prime_UTR
ATGATGAAGGGTTTGTGATT+TGG 0.301555 7_4:-9885932 None:intergenic
AAGATGGGGTGGTTGTGGTG+TGG 0.309946 7_4:-9885800 None:intergenic
GAGGTTGGTGGTGGCTGTGA+TGG 0.311859 7_4:-9885717 None:intergenic
ATCTCTTCTGTGTGTCTAAT+TGG 0.317547 7_4:+9887855 Msa1139460:three_prime_UTR
GTGGTGGTGGTGGAGGTTGG+TGG 0.319503 7_4:-9885729 None:intergenic
GAGGTGGTGGTGGTGGAGGT+TGG 0.323697 7_4:-9885732 None:intergenic
GTGGTGGTGGAGGTTGGTGG+TGG 0.332471 7_4:-9885726 None:intergenic
AGATGGGGTGGTTGTGGTGT+GGG 0.335949 7_4:-9885799 None:intergenic
CAATGGGTTGGAGGAGGTGG+TGG 0.351890 7_4:-9885745 None:intergenic
GGGGTGTAGCATGGGTAAGA+GGG 0.352158 7_4:-9885778 None:intergenic
AAGGTCCATTAGTAGAGATT+TGG 0.356909 7_4:+9887034 Msa1139460:CDS
AGATGTTGTGGCCACTTCTC+AGG 0.366175 7_4:+9887535 Msa1139460:CDS
ATGGGGTGGTTGTGGTGTGG+GGG 0.370454 7_4:-9885797 None:intergenic
GGGTGTAGCATGGGTAAGAG+GGG 0.380958 7_4:-9885777 None:intergenic
CTGAACCAAATCTCTACTAA+TGG 0.382106 7_4:-9887039 None:intergenic
GGAAAGAACGGAAATGGGTT+TGG 0.385477 7_4:-9886074 None:intergenic
GAACGAGGAAAGAACGGAAA+TGG 0.391524 7_4:-9886080 None:intergenic
GTGCAGTAGCAATGGGTTGG+AGG 0.393924 7_4:-9885754 None:intergenic
CATTACATTATATTCATGTA+AGG 0.395458 7_4:+9887751 Msa1139460:three_prime_UTR
GTCGTGTTGTGGAGAAAGAT+GGG 0.404514 7_4:-9885815 None:intergenic
GTTGGTGGTGGCTGTGATGG+TGG 0.408088 7_4:-9885714 None:intergenic
GGAGAAAGATGGGGTGGTTG+TGG 0.411329 7_4:-9885805 None:intergenic
TAGCAATGGGTTGGAGGAGG+TGG 0.415086 7_4:-9885748 None:intergenic
AGAAAGGAGGATGGAGGAAA+TGG 0.423348 7_4:-9885851 None:intergenic
GAAGATTAGCAAATTGTGTA+AGG 0.423404 7_4:+9887223 Msa1139460:CDS
CTCGCCTTGGTCATCATTTG+AGG 0.425827 7_4:-9887483 None:intergenic
ATCACATATAATCACAACTT+TGG 0.426004 7_4:+9888013 Msa1139460:three_prime_UTR
AAAGAGCTGGAAAATCGAAA+TGG 0.431350 7_4:-9886191 None:intergenic
GTTGATTGAGGGAAGATTCA+AGG 0.442554 7_4:-9886002 None:intergenic
GGTCGTGTTGTGGAGAAAGA+TGG 0.442953 7_4:-9885816 None:intergenic
GGCTGTGATGGTGGTTGTAG+TGG 0.443051 7_4:-9885705 None:intergenic
GGAGAGAAGAGAGTGTAGAT+TGG 0.446189 7_4:-9885963 None:intergenic
AACGAGGAAAGAACGGAAAT+GGG 0.452174 7_4:-9886079 None:intergenic
AGAAGCTGTTGAAGAATTTG+AGG 0.452656 7_4:+9887189 Msa1139460:CDS
GGTGGTTGAGTTGTGGAGAA+AGG 0.452850 7_4:-9885867 None:intergenic
GGTGTAGCATGGGTAAGAGG+GGG 0.467114 7_4:-9885776 None:intergenic
ATCAAACATTACAAATCAAA+GGG 0.474368 7_4:-9887783 None:intergenic
TGGAGAAGGTTTAGCGAATG+AGG 0.477760 7_4:+9887259 Msa1139460:CDS
CAATGATCGTGGATGGGAAA+AGG 0.480914 7_4:+9887015 Msa1139460:CDS
TAACTCCATGATTGTTGAGA+AGG 0.481847 7_4:-9887953 None:intergenic
AGTGTGAGATTTGCAGAGAA+TGG 0.482476 7_4:+9887410 Msa1139460:CDS
TGTAAGGTTTATGGAGATGA+TGG 0.484958 7_4:+9887239 Msa1139460:CDS
TCAGCCCTTCTCAACAATCA+TGG 0.485463 7_4:+9887948 Msa1139460:three_prime_UTR
GAAGGGTTTGTGATTTGGGG+TGG 0.485747 7_4:-9885927 None:intergenic
GATGAGTTTGATGTTGATTG+AGG 0.494626 7_4:-9886014 None:intergenic
CAGAGAACAAAATCTTCTCT+TGG 0.499499 7_4:-9887655 None:intergenic
GAAGCTGTTGAAGAATTTGA+GGG 0.504399 7_4:+9887190 Msa1139460:CDS
TGGTGTCTTTGTTCAAAGGC+AGG 0.507089 7_4:+9887364 Msa1139460:CDS
AAAATGGTGTCTTTGTTCAA+AGG 0.507657 7_4:+9887360 Msa1139460:CDS
GCGGTAAAGAGTTCGAGGTC+TGG 0.513210 7_4:+9887116 Msa1139460:CDS
ATCTACGAACGAGGAAAGAA+CGG 0.513538 7_4:-9886086 None:intergenic
TAGCACAAGAACTTGTGAAT+CGG 0.513547 7_4:+9887451 Msa1139460:CDS
AATCAAACATTACAAATCAA+AGG 0.515244 7_4:-9887784 None:intergenic
GGTGGTCGTGTACGAGGTTG+TGG 0.516181 7_4:-9885897 None:intergenic
AAGAGCTGGAAAATCGAAAT+GGG 0.517094 7_4:-9886190 None:intergenic
GAGTTGTGGAGAAAGGAGGA+TGG 0.519893 7_4:-9885860 None:intergenic
CTCACACTCTTCTTTACTCT+AGG 0.520724 7_4:-9887395 None:intergenic
AGAACTGATTTGAAGAGTAA+AGG 0.524279 7_4:+9887704 Msa1139460:CDS
CAGTAGCAATGGGTTGGAGG+AGG 0.524583 7_4:-9885751 None:intergenic
CAAGGGTTTCAATGCGGTGA+AGG 0.527850 7_4:-9885984 None:intergenic
TTGATTGAGGGAAGATTCAA+GGG 0.529353 7_4:-9886001 None:intergenic
TATTCTTCAAAACCTCGCCT+TGG 0.532468 7_4:-9887496 None:intergenic
CTAATAAACAACAATTGAAG+TGG 0.533165 7_4:-9887909 None:intergenic
AACACTCACACTCAGCTGAA+TGG 0.543056 7_4:+9885672 Msa1139460:exon
AGATCTAATAGCAATGAAAG+AGG 0.544021 7_4:-9886151 None:intergenic
TCAACACGTAGTAGTGATGA+TGG 0.546972 7_4:+9887602 Msa1139460:CDS
GTGATTTGGGGTGGTGTACG+AGG 0.550229 7_4:-9885918 None:intergenic
ATGAGTTTGATGTTGATTGA+GGG 0.550394 7_4:-9886013 None:intergenic
AACTCCATGATTGTTGAGAA+GGG 0.554944 7_4:-9887952 None:intergenic
TGTACGAGGTGGTTGAGTTG+TGG 0.556062 7_4:-9885874 None:intergenic
GTTTATGGAGATGATGGAGA+AGG 0.561130 7_4:+9887245 Msa1139460:CDS
TGACGAGGAAGTGCTGGTGG+AGG 0.561276 7_4:+9887568 Msa1139460:CDS
GATGAAGGGTTTGTGATTTG+GGG 0.561923 7_4:-9885930 None:intergenic
TGAAGATGACGAGGAAGTGC+TGG 0.561932 7_4:+9887562 Msa1139460:CDS
TTGTGGAGAAAGGAGGATGG+AGG 0.563095 7_4:-9885857 None:intergenic
TGTGATGGTGGTTGTAGTGG+TGG 0.564747 7_4:-9885702 None:intergenic
ATCTTCAACACCCTGAGAAG+TGG 0.565897 7_4:-9887546 None:intergenic
TTAAAGCGGTAAAGAGTTCG+AGG 0.566611 7_4:+9887111 Msa1139460:CDS
TGTACGAGGTGGTCGTGTTG+TGG 0.568594 7_4:-9885826 None:intergenic
GTGTGAGTGTTACTCCCTCA+AGG 0.570318 7_4:-9885660 None:intergenic
GATGTTGTGGCCACTTCTCA+GGG 0.573838 7_4:+9887536 Msa1139460:CDS
AGCTGTTGAAGAATTTGAGG+GGG 0.574755 7_4:+9887192 Msa1139460:CDS
GTTTGCAGTTGTAGATGTTG+TGG 0.582194 7_4:+9887523 Msa1139460:CDS
ACAGCTTTGAGAGAAAGAGC+TGG 0.591007 7_4:-9886204 None:intergenic
GGGGTGCAGTAGCAATGGGT+TGG 0.592304 7_4:-9885757 None:intergenic
TGTGATACAATGATCGTGGA+TGG 0.592819 7_4:+9887008 Msa1139460:CDS
TTCGTAGATCAAGAACACTA+AGG 0.594526 7_4:+9886100 Msa1139460:CDS
GGTTGAGTTGTGGAGAAAGG+AGG 0.595377 7_4:-9885864 None:intergenic
GCATATGCATTATGTTAAAG+CGG 0.601940 7_4:+9887097 Msa1139460:CDS
TTACACGTGCTGCGGAGTGT+CGG 0.611191 7_4:-9886050 None:intergenic
GTGGTTGTAGTGGTGGTGAG+AGG 0.617629 7_4:-9885695 None:intergenic
AAGCTGTTGAAGAATTTGAG+GGG 0.617769 7_4:+9887191 Msa1139460:CDS
CTCAAATGATGACCAAGGCG+AGG 0.628250 7_4:+9887484 Msa1139460:CDS
TGTGAGTGTTACTCCCTCAA+GGG 0.634914 7_4:-9885659 None:intergenic
CAGAGACCAAGAAAACCAGA+AGG 0.649951 7_4:+9887149 Msa1139460:CDS
ACAAGAACTTGTGAATCGGA+TGG 0.651774 7_4:+9887455 Msa1139460:CDS
TGTTGTGGAGAAAGATGGGG+TGG 0.652811 7_4:-9885811 None:intergenic
TCGTGTTGTGGAGAAAGATG+GGG 0.652928 7_4:-9885814 None:intergenic
AGAGAATGGTAATGTTTGTG+AGG 0.654824 7_4:+9887424 Msa1139460:CDS
GGTTTGGATTACACGTGCTG+CGG 0.656835 7_4:-9886058 None:intergenic
GAAGTGCTGGTGGAGGACAG+TGG 0.658352 7_4:+9887575 Msa1139460:CDS
GTGATACAATGATCGTGGAT+GGG 0.659335 7_4:+9887009 Msa1139460:CDS
AGATGACGAGGAAGTGCTGG+TGG 0.672090 7_4:+9887565 Msa1139460:CDS
AGGAAATGGTTTGTACGAGG+TGG 0.672405 7_4:-9885837 None:intergenic
GTGCTGGTGGAGGACAGTGG+AGG 0.676247 7_4:+9887578 Msa1139460:CDS
GTACGAGGTGGTCGTGTACG+AGG 0.676857 7_4:-9885903 None:intergenic
TGGAGGAAATGGTTTGTACG+AGG 0.681151 7_4:-9885840 None:intergenic
GACGATGATTATGATGACGA+TGG 0.683290 7_4:+9887299 Msa1139460:CDS
GGACTGTGATACAATGATCG+TGG 0.688220 7_4:+9887004 Msa1139460:intron
TCAGGGTGTTGAAGATGACG+AGG 0.691426 7_4:+9887553 Msa1139460:CDS
AGTTCCTCAAATGATGACCA+AGG 0.698816 7_4:+9887479 Msa1139460:CDS
GTGTTCTTGATCTACGAACG+AGG 0.702234 7_4:-9886095 None:intergenic
GTACGAGGTTGTGGTGTACG+AGG 0.710331 7_4:-9885888 None:intergenic
ATTTGGGGTGGTGTACGAGG+TGG 0.713420 7_4:-9885915 None:intergenic
AAGATTCAAGGGTTTCAATG+CGG 0.722631 7_4:-9885990 None:intergenic
CGAGGTTGTGGTGTACGAGG+TGG 0.734134 7_4:-9885885 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCAAATTTTTATTTTCA+GGG - chr7_4:9886337-9886356 None:intergenic 10.0%
!!! AATTTCAAATTTTTATTTTC+AGG - chr7_4:9886338-9886357 None:intergenic 10.0%
!!! GAAAATAAAAATTTGAAATT+TGG + chr7_4:9886338-9886357 Msa1139460:intron 10.0%
!!! TTTTATTTTGAATTTTTTAG+GGG + chr7_4:9886412-9886431 Msa1139460:intron 10.0%
!!! AAAATTTGAAATTTGGTTTT+AGG + chr7_4:9886345-9886364 Msa1139460:intron 15.0%
!!! AAATTTGAAATTTGGTTTTA+GGG + chr7_4:9886346-9886365 Msa1139460:intron 15.0%
!!! TTTATTTTGAATTTTTTAGG+GGG + chr7_4:9886413-9886432 Msa1139460:intron 15.0%
!!! ATTTCAAATTTTCATTTTCA+TGG - chr7_4:9886458-9886477 None:intergenic 15.0%
!!! AAAATTTGAAATTTAGCTTT+AGG + chr7_4:9886466-9886485 Msa1139460:intron 15.0%
!!! AAATTTGAAATTTAGCTTTA+GGG + chr7_4:9886467-9886486 Msa1139460:intron 15.0%
!!! TTTCATTTGTGTTTTTTTTG+AGG + chr7_4:9886290-9886309 Msa1139460:intron 20.0%
!! TAAATTACAATGATTGTGAA+TGG + chr7_4:9886377-9886396 Msa1139460:intron 20.0%
!! AAATTACAATGATTGTGAAT+GGG + chr7_4:9886378-9886397 Msa1139460:intron 20.0%
!!! GATACTTTTAACGATATTTT+AGG + chr7_4:9886589-9886608 Msa1139460:intron 20.0%
!!! GTAAAAATGTTTTACACTAT+TGG + chr7_4:9886658-9886677 Msa1139460:intron 20.0%
!!! GATATATGTAGAGATATTTT+AGG + chr7_4:9886794-9886813 Msa1139460:intron 20.0%
!!! GTAAAGAATTTTTACAATGT+CGG + chr7_4:9886864-9886883 Msa1139460:intron 20.0%
!! AATTTGAATCCATAGATAAT+TGG + chr7_4:9886894-9886913 Msa1139460:intron 20.0%
!! AAAATAGAACCAATTATCTA+TGG - chr7_4:9886906-9886925 None:intergenic 20.0%
!! CATTACATTATATTCATGTA+AGG + chr7_4:9887751-9887770 Msa1139460:three_prime_UTR 20.0%
!! ATCAAACATTACAAATCAAA+GGG - chr7_4:9887786-9887805 None:intergenic 20.0%
!! AATCAAACATTACAAATCAA+AGG - chr7_4:9887787-9887806 None:intergenic 20.0%
!! AACTTAGAATGTTTAGTAAT+TGG + chr7_4:9887829-9887848 Msa1139460:three_prime_UTR 20.0%
! AAATGAAAAATACCCAAGAT+TGG - chr7_4:9886267-9886286 None:intergenic 25.0%
! GATTAATTTCTCTTTGTTGA+CGG + chr7_4:9886521-9886540 Msa1139460:intron 25.0%
! TCTATTTGTCATGATTTGAT+AGG - chr7_4:9886776-9886795 None:intergenic 25.0%
! CTTCATGACATAAATAATAG+AGG - chr7_4:9886960-9886979 None:intergenic 25.0%
!!! GAAGAGAAAGTTTTGAAATT+TGG + chr7_4:9886976-9886995 Msa1139460:intron 25.0%
!!! AAGTTTTGAAATTTGGCTTT+AGG + chr7_4:9886983-9887002 Msa1139460:intron 25.0%
! GTAATGTTTGATTGTATTCT+AGG + chr7_4:9887794-9887813 Msa1139460:three_prime_UTR 25.0%
! GAACATAACAAATCCATTTA+TGG - chr7_4:9887890-9887909 None:intergenic 25.0%
! CTAATAAACAACAATTGAAG+TGG - chr7_4:9887912-9887931 None:intergenic 25.0%
! ATCACATATAATCACAACTT+TGG + chr7_4:9888013-9888032 Msa1139460:three_prime_UTR 25.0%
!!! TTGGTGATTTTGATGATGAA+GGG - chr7_4:9885947-9885966 None:intergenic 30.0%
!!! ATTGGTGATTTTGATGATGA+AGG - chr7_4:9885948-9885967 None:intergenic 30.0%
! ATGAGTTTGATGTTGATTGA+GGG - chr7_4:9886016-9886035 None:intergenic 30.0%
TGATAGATGCAAGTTGTTTA+AGG - chr7_4:9886128-9886147 None:intergenic 30.0%
AGATCTAATAGCAATGAAAG+AGG - chr7_4:9886154-9886173 None:intergenic 30.0%
CATCAAACTTGATTCAATTC+AGG + chr7_4:9886236-9886255 Msa1139460:CDS 30.0%
AATTCAGGTAAATCCAATCT+TGG + chr7_4:9886251-9886270 Msa1139460:intron 30.0%
ATTCAGGTAAATCCAATCTT+GGG + chr7_4:9886252-9886271 Msa1139460:intron 30.0%
TAGCATCAAGTGATTTGATT+AGG + chr7_4:9886628-9886647 Msa1139460:intron 30.0%
CAAACTAAATTCATTGCAGA+AGG + chr7_4:9886687-9886706 Msa1139460:intron 30.0%
! GTGTAAACATTTTACACTCT+CGG - chr7_4:9886747-9886766 None:intergenic 30.0%
!! TAGAGATTTGGTTCAGTTTT+TGG + chr7_4:9887046-9887065 Msa1139460:CDS 30.0%
GCATATGCATTATGTTAAAG+CGG + chr7_4:9887097-9887116 Msa1139460:CDS 30.0%
GAAGATTAGCAAATTGTGTA+AGG + chr7_4:9887223-9887242 Msa1139460:CDS 30.0%
AGCAAATTGTGTAAGGTTTA+TGG + chr7_4:9887230-9887249 Msa1139460:CDS 30.0%
!!! TGATGTTTTGATTGGTAGAA+GGG + chr7_4:9887328-9887347 Msa1139460:CDS 30.0%
AAAATGGTGTCTTTGTTCAA+AGG + chr7_4:9887360-9887379 Msa1139460:CDS 30.0%
!! AGAACTGATTTGAAGAGTAA+AGG + chr7_4:9887704-9887723 Msa1139460:CDS 30.0%
AGAAAAACAATAGTCCCTTG+AGG + chr7_4:9885645-9885664 Msa1139460:five_prime_UTR 35.0%
GAAAAACAATAGTCCCTTGA+GGG + chr7_4:9885646-9885665 Msa1139460:five_prime_UTR 35.0%
TGATGAAGGGTTTGTGATTT+GGG - chr7_4:9885934-9885953 None:intergenic 35.0%
ATGATGAAGGGTTTGTGATT+TGG - chr7_4:9885935-9885954 None:intergenic 35.0%
AAGATTCAAGGGTTTCAATG+CGG - chr7_4:9885993-9886012 None:intergenic 35.0%
TTGATTGAGGGAAGATTCAA+GGG - chr7_4:9886004-9886023 None:intergenic 35.0%
! GATGAGTTTGATGTTGATTG+AGG - chr7_4:9886017-9886036 None:intergenic 35.0%
TTCGTAGATCAAGAACACTA+AGG + chr7_4:9886100-9886119 Msa1139460:CDS 35.0%
AAGAGCTGGAAAATCGAAAT+GGG - chr7_4:9886193-9886212 None:intergenic 35.0%
AAAGAGCTGGAAAATCGAAA+TGG - chr7_4:9886194-9886213 None:intergenic 35.0%
GGGTTGTGATACAATGATTA+TGG + chr7_4:9886487-9886506 Msa1139460:intron 35.0%
!! CACTCAATGATGCACTTTTT+GGG - chr7_4:9886826-9886845 None:intergenic 35.0%
! TCACTCAATGATGCACTTTT+TGG - chr7_4:9886827-9886846 None:intergenic 35.0%
AAGGTCCATTAGTAGAGATT+TGG + chr7_4:9887034-9887053 Msa1139460:CDS 35.0%
CTGAACCAAATCTCTACTAA+TGG - chr7_4:9887042-9887061 None:intergenic 35.0%
!! GTTTTTGGACTCAATTGAAG+AGG + chr7_4:9887061-9887080 Msa1139460:CDS 35.0%
AGAAGCTGTTGAAGAATTTG+AGG + chr7_4:9887189-9887208 Msa1139460:CDS 35.0%
GAAGCTGTTGAAGAATTTGA+GGG + chr7_4:9887190-9887209 Msa1139460:CDS 35.0%
AAGCTGTTGAAGAATTTGAG+GGG + chr7_4:9887191-9887210 Msa1139460:CDS 35.0%
TGTAAGGTTTATGGAGATGA+TGG + chr7_4:9887239-9887258 Msa1139460:CDS 35.0%
!!! GGAGTAACTGATGTTTTGAT+TGG + chr7_4:9887320-9887339 Msa1139460:CDS 35.0%
!!! CTGATGTTTTGATTGGTAGA+AGG + chr7_4:9887327-9887346 Msa1139460:CDS 35.0%
AGAAGGGATAGCAACAAAAA+TGG + chr7_4:9887344-9887363 Msa1139460:CDS 35.0%
! AGAGAATGGTAATGTTTGTG+AGG + chr7_4:9887424-9887443 Msa1139460:CDS 35.0%
TAGCACAAGAACTTGTGAAT+CGG + chr7_4:9887451-9887470 Msa1139460:CDS 35.0%
CAGAGAACAAAATCTTCTCT+TGG - chr7_4:9887658-9887677 None:intergenic 35.0%
!! CTTTCCATTTTAAGTGGCAA+TGG - chr7_4:9887683-9887702 None:intergenic 35.0%
! GTTCTGCTTTCCATTTTAAG+TGG - chr7_4:9887689-9887708 None:intergenic 35.0%
ATCTCTTCTGTGTGTCTAAT+TGG + chr7_4:9887855-9887874 Msa1139460:three_prime_UTR 35.0%
! TTGGCATTTGGTTCCATAAA+TGG + chr7_4:9887874-9887893 Msa1139460:three_prime_UTR 35.0%
AACTCCATGATTGTTGAGAA+GGG - chr7_4:9887955-9887974 None:intergenic 35.0%
TAACTCCATGATTGTTGAGA+AGG - chr7_4:9887956-9887975 None:intergenic 35.0%
GATGAAGGGTTTGTGATTTG+GGG - chr7_4:9885933-9885952 None:intergenic 40.0%
GTTGATTGAGGGAAGATTCA+AGG - chr7_4:9886005-9886024 None:intergenic 40.0%
AACGAGGAAAGAACGGAAAT+GGG - chr7_4:9886082-9886101 None:intergenic 40.0%
ATCTACGAACGAGGAAAGAA+CGG - chr7_4:9886089-9886108 None:intergenic 40.0%
TGTGATACAATGATCGTGGA+TGG + chr7_4:9887008-9887027 Msa1139460:CDS 40.0%
GTGATACAATGATCGTGGAT+GGG + chr7_4:9887009-9887028 Msa1139460:CDS 40.0%
TTAAAGCGGTAAAGAGTTCG+AGG + chr7_4:9887111-9887130 Msa1139460:CDS 40.0%
!!! TCATCACCTTCTGGTTTTCT+TGG - chr7_4:9887158-9887177 None:intergenic 40.0%
TTCTCATCATCATCACCTTC+TGG - chr7_4:9887167-9887186 None:intergenic 40.0%
AGCTGTTGAAGAATTTGAGG+GGG + chr7_4:9887192-9887211 Msa1139460:CDS 40.0%
!! GTTTATGGAGATGATGGAGA+AGG + chr7_4:9887245-9887264 Msa1139460:CDS 40.0%
! GACGATGATTATGATGACGA+TGG + chr7_4:9887299-9887318 Msa1139460:CDS 40.0%
CTCACACTCTTCTTTACTCT+AGG - chr7_4:9887398-9887417 None:intergenic 40.0%
AGTGTGAGATTTGCAGAGAA+TGG + chr7_4:9887410-9887429 Msa1139460:CDS 40.0%
ACAAGAACTTGTGAATCGGA+TGG + chr7_4:9887455-9887474 Msa1139460:CDS 40.0%
AGTTCCTCAAATGATGACCA+AGG + chr7_4:9887479-9887498 Msa1139460:CDS 40.0%
TATTCTTCAAAACCTCGCCT+TGG - chr7_4:9887499-9887518 None:intergenic 40.0%
GTTTGCAGTTGTAGATGTTG+TGG + chr7_4:9887523-9887542 Msa1139460:CDS 40.0%
TCAACACGTAGTAGTGATGA+TGG + chr7_4:9887602-9887621 Msa1139460:CDS 40.0%
! TGCTCCATTGCCACTTAAAA+TGG + chr7_4:9887676-9887695 Msa1139460:CDS 40.0%
CTGTGTGTCTAATTGGCATT+TGG + chr7_4:9887862-9887881 Msa1139460:three_prime_UTR 40.0%
! TGTGAGTGTTACTCCCTCAA+GGG - chr7_4:9885662-9885681 None:intergenic 45.0%
AACACTCACACTCAGCTGAA+TGG + chr7_4:9885672-9885691 Msa1139460:exon 45.0%
TCGTGTTGTGGAGAAAGATG+GGG - chr7_4:9885817-9885836 None:intergenic 45.0%
GTCGTGTTGTGGAGAAAGAT+GGG - chr7_4:9885818-9885837 None:intergenic 45.0%
! AGGAAATGGTTTGTACGAGG+TGG - chr7_4:9885840-9885859 None:intergenic 45.0%
! TGGAGGAAATGGTTTGTACG+AGG - chr7_4:9885843-9885862 None:intergenic 45.0%
AGAAAGGAGGATGGAGGAAA+TGG - chr7_4:9885854-9885873 None:intergenic 45.0%
! GGAGAGAAGAGAGTGTAGAT+TGG - chr7_4:9885966-9885985 None:intergenic 45.0%
! GGAAAGAACGGAAATGGGTT+TGG - chr7_4:9886077-9886096 None:intergenic 45.0%
GAACGAGGAAAGAACGGAAA+TGG - chr7_4:9886083-9886102 None:intergenic 45.0%
GTGTTCTTGATCTACGAACG+AGG - chr7_4:9886098-9886117 None:intergenic 45.0%
!! ACAGCTTTGAGAGAAAGAGC+TGG - chr7_4:9886207-9886226 None:intergenic 45.0%
GGACTGTGATACAATGATCG+TGG + chr7_4:9887004-9887023 Msa1139460:intron 45.0%
CAATGATCGTGGATGGGAAA+AGG + chr7_4:9887015-9887034 Msa1139460:CDS 45.0%
CAGAGACCAAGAAAACCAGA+AGG + chr7_4:9887149-9887168 Msa1139460:CDS 45.0%
TGTTGAAGAATTTGAGGGGG+AGG + chr7_4:9887195-9887214 Msa1139460:CDS 45.0%
GTTGAAGAATTTGAGGGGGA+GGG + chr7_4:9887196-9887215 Msa1139460:CDS 45.0%
TGGAGAAGGTTTAGCGAATG+AGG + chr7_4:9887259-9887278 Msa1139460:CDS 45.0%
TGGTGTCTTTGTTCAAAGGC+AGG + chr7_4:9887364-9887383 Msa1139460:CDS 45.0%
ATCTTCAACACCCTGAGAAG+TGG - chr7_4:9887549-9887568 None:intergenic 45.0%
TCAGCCCTTCTCAACAATCA+TGG + chr7_4:9887948-9887967 Msa1139460:three_prime_UTR 45.0%
!!! TATTTTATTTTGAATTTTTT+AGG + chr7_4:9886410-9886429 Msa1139460:intron 5.0%
!!! ATTTTATTTTGAATTTTTTA+GGG + chr7_4:9886411-9886430 Msa1139460:intron 5.0%
!!! TAAATTTTTTTTTTTTTTTG+AGG + chr7_4:9886929-9886948 Msa1139460:intron 5.0%
GAGAGAGAGAGAGAGAGAGA+GGG - chr7_4:9885585-9885604 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG - chr7_4:9885584-9885603 None:intergenic 50.0%
! GTGTGAGTGTTACTCCCTCA+AGG - chr7_4:9885663-9885682 None:intergenic 50.0%
!! TGTGATGGTGGTTGTAGTGG+TGG - chr7_4:9885705-9885724 None:intergenic 50.0%
TGTTGTGGAGAAAGATGGGG+TGG - chr7_4:9885814-9885833 None:intergenic 50.0%
GGTCGTGTTGTGGAGAAAGA+TGG - chr7_4:9885819-9885838 None:intergenic 50.0%
TTGTGGAGAAAGGAGGATGG+AGG - chr7_4:9885860-9885879 None:intergenic 50.0%
GAGTTGTGGAGAAAGGAGGA+TGG - chr7_4:9885863-9885882 None:intergenic 50.0%
! GGTTGAGTTGTGGAGAAAGG+AGG - chr7_4:9885867-9885886 None:intergenic 50.0%
! GGTGGTTGAGTTGTGGAGAA+AGG - chr7_4:9885870-9885889 None:intergenic 50.0%
! TGTACGAGGTGGTTGAGTTG+TGG - chr7_4:9885877-9885896 None:intergenic 50.0%
! GAAGGGTTTGTGATTTGGGG+TGG - chr7_4:9885930-9885949 None:intergenic 50.0%
CAAGGGTTTCAATGCGGTGA+AGG - chr7_4:9885987-9886006 None:intergenic 50.0%
GGTTTGGATTACACGTGCTG+CGG - chr7_4:9886061-9886080 None:intergenic 50.0%
CTCGCCTTGGTCATCATTTG+AGG - chr7_4:9887486-9887505 None:intergenic 50.0%
CTCAAATGATGACCAAGGCG+AGG + chr7_4:9887484-9887503 Msa1139460:CDS 50.0%
AGATGTTGTGGCCACTTCTC+AGG + chr7_4:9887535-9887554 Msa1139460:CDS 50.0%
GATGTTGTGGCCACTTCTCA+GGG + chr7_4:9887536-9887555 Msa1139460:CDS 50.0%
!! TCAGGGTGTTGAAGATGACG+AGG + chr7_4:9887553-9887572 Msa1139460:CDS 50.0%
! TGAAGATGACGAGGAAGTGC+TGG + chr7_4:9887562-9887581 Msa1139460:CDS 50.0%
! GTGGTTGTAGTGGTGGTGAG+AGG - chr7_4:9885698-9885717 None:intergenic 55.0%
!! GGCTGTGATGGTGGTTGTAG+TGG - chr7_4:9885708-9885727 None:intergenic 55.0%
!! TAGCAATGGGTTGGAGGAGG+TGG - chr7_4:9885751-9885770 None:intergenic 55.0%
!! CAGTAGCAATGGGTTGGAGG+AGG - chr7_4:9885754-9885773 None:intergenic 55.0%
!! GTGCAGTAGCAATGGGTTGG+AGG - chr7_4:9885757-9885776 None:intergenic 55.0%
! AGAGGGGGTGCAGTAGCAAT+GGG - chr7_4:9885764-9885783 None:intergenic 55.0%
! AAGAGGGGGTGCAGTAGCAA+TGG - chr7_4:9885765-9885784 None:intergenic 55.0%
GGTGTAGCATGGGTAAGAGG+GGG - chr7_4:9885779-9885798 None:intergenic 55.0%
GGGTGTAGCATGGGTAAGAG+GGG - chr7_4:9885780-9885799 None:intergenic 55.0%
GGGGTGTAGCATGGGTAAGA+GGG - chr7_4:9885781-9885800 None:intergenic 55.0%
AGATGGGGTGGTTGTGGTGT+GGG - chr7_4:9885802-9885821 None:intergenic 55.0%
AAGATGGGGTGGTTGTGGTG+TGG - chr7_4:9885803-9885822 None:intergenic 55.0%
GGAGAAAGATGGGGTGGTTG+TGG - chr7_4:9885808-9885827 None:intergenic 55.0%
TGTACGAGGTGGTCGTGTTG+TGG - chr7_4:9885829-9885848 None:intergenic 55.0%
GTACGAGGTTGTGGTGTACG+AGG - chr7_4:9885891-9885910 None:intergenic 55.0%
! ATTTGGGGTGGTGTACGAGG+TGG - chr7_4:9885918-9885937 None:intergenic 55.0%
! GTGATTTGGGGTGGTGTACG+AGG - chr7_4:9885921-9885940 None:intergenic 55.0%
TTACACGTGCTGCGGAGTGT+CGG - chr7_4:9886053-9886072 None:intergenic 55.0%
GCGGTAAAGAGTTCGAGGTC+TGG + chr7_4:9887116-9887135 Msa1139460:CDS 55.0%
!! AGATGACGAGGAAGTGCTGG+TGG + chr7_4:9887565-9887584 Msa1139460:CDS 55.0%
!! GTTGGTGGTGGCTGTGATGG+TGG - chr7_4:9885717-9885736 None:intergenic 60.0%
!! GAGGTTGGTGGTGGCTGTGA+TGG - chr7_4:9885720-9885739 None:intergenic 60.0%
!! CAATGGGTTGGAGGAGGTGG+TGG - chr7_4:9885748-9885767 None:intergenic 60.0%
GGGGTGCAGTAGCAATGGGT+TGG - chr7_4:9885760-9885779 None:intergenic 60.0%
! GGGGGTGTAGCATGGGTAAG+AGG - chr7_4:9885782-9885801 None:intergenic 60.0%
! GTGGTGTGGGGGTGTAGCAT+GGG - chr7_4:9885789-9885808 None:intergenic 60.0%
! TGTGGTGTGGGGGTGTAGCA+TGG - chr7_4:9885790-9885809 None:intergenic 60.0%
ATGGGGTGGTTGTGGTGTGG+GGG - chr7_4:9885800-9885819 None:intergenic 60.0%
GATGGGGTGGTTGTGGTGTG+GGG - chr7_4:9885801-9885820 None:intergenic 60.0%
CGAGGTTGTGGTGTACGAGG+TGG - chr7_4:9885888-9885907 None:intergenic 60.0%
GGTGGTCGTGTACGAGGTTG+TGG - chr7_4:9885900-9885919 None:intergenic 60.0%
GTACGAGGTGGTCGTGTACG+AGG - chr7_4:9885906-9885925 None:intergenic 60.0%
!! TGACGAGGAAGTGCTGGTGG+AGG + chr7_4:9887568-9887587 Msa1139460:CDS 60.0%
!! GAAGTGCTGGTGGAGGACAG+TGG + chr7_4:9887575-9887594 Msa1139460:CDS 60.0%
!! GTGGTGGTGGAGGTTGGTGG+TGG - chr7_4:9885729-9885748 None:intergenic 65.0%
!! GTGGTGGTGGTGGAGGTTGG+TGG - chr7_4:9885732-9885751 None:intergenic 65.0%
!! GAGGTGGTGGTGGTGGAGGT+TGG - chr7_4:9885735-9885754 None:intergenic 65.0%
!! GTTGGAGGAGGTGGTGGTGG+TGG - chr7_4:9885742-9885761 None:intergenic 65.0%
! TGGGTTGGAGGAGGTGGTGG+TGG - chr7_4:9885745-9885764 None:intergenic 65.0%
! GTGCTGGTGGAGGACAGTGG+AGG + chr7_4:9887578-9887597 Msa1139460:CDS 65.0%
!! GGAGGAGGTGGTGGTGGTGG+AGG - chr7_4:9885739-9885758 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 9885582 9888037 9885582 ID=Msa1139460;Name=Msa1139460
chr7_4 mRNA 9885582 9888037 9885582 ID=Msa1139460-mRNA-1;Parent=Msa1139460;Name=Msa1139460-mRNA-1;_AED=0.05;_eAED=0.05;_QI=109|1|1|1|1|1|2|292|435
chr7_4 exon 9885582 9886257 9885582 ID=Msa1139460-mRNA-1:exon:2483;Parent=Msa1139460-mRNA-1
chr7_4 exon 9887005 9888037 9887005 ID=Msa1139460-mRNA-1:exon:2484;Parent=Msa1139460-mRNA-1
chr7_4 five_prime_UTR 9885582 9885690 9885582 ID=Msa1139460-mRNA-1:five_prime_utr;Parent=Msa1139460-mRNA-1
chr7_4 CDS 9885691 9886257 9885691 ID=Msa1139460-mRNA-1:cds;Parent=Msa1139460-mRNA-1
chr7_4 CDS 9887005 9887745 9887005 ID=Msa1139460-mRNA-1:cds;Parent=Msa1139460-mRNA-1
chr7_4 three_prime_UTR 9887746 9888037 9887746 ID=Msa1139460-mRNA-1:three_prime_utr;Parent=Msa1139460-mRNA-1
Gene Sequence

>Msa1139460

ATGGCCTCTCACCACCACTACAACCACCATCACAGCCACCACCAACCTCCACCACCACCACCTCCTCCAACCCATTGCTACTGCACCCCCTCTTACCCATGCTACACCCCCACACCACAACCACCCCATCTTTCTCCACAACACGACCACCTCGTACAAACCATTTCCTCCATCCTCCTTTCTCCACAACTCAACCACCTCGTACACCACAACCTCGTACACGACCACCTCGTACACCACCCCAAATCACAAACCCTTCATCATCAAAATCACCAATCTACACTCTCTTCTCTCCTTCACCGCATTGAAACCCTTGAATCTTCCCTCAATCAACATCAAACTCATCAATCTCAATCTCTCCGACACTCCGCAGCACGTGTAATCCAAACCCATTTCCGTTCTTTCCTCGTTCGTAGATCAAGAACACTAAGGAACCTTAAACAACTTGCATCTATCAAATCCTCTTTCATTGCTATTAGATCTTCGTTTTCTACGCATACCCATTTCGATTTTCCAGCTCTTTCTCTCAAAGCTGTTAACTTGCTCATCAAACTTGATTCAATTCAGGACTGTGATACAATGATCGTGGATGGGAAAAGGTCCATTAGTAGAGATTTGGTTCAGTTTTTGGACTCAATTGAAGAGGTTGCTGCGAAAAAGCATATGCATTATGTTAAAGCGGTAAAGAGTTCGAGGTCTGGTCAGAAAGTTCAGAGACCAAGAAAACCAGAAGGTGATGATGATGAGAAGAAGAAGCTGTTGAAGAATTTGAGGGGGAGGGTTGAGAAGATTAGCAAATTGTGTAAGGTTTATGGAGATGATGGAGAAGGTTTAGCGAATGAGGAGAGCATTCATGATCATGACGATGATTATGATGACGATGGAGTAACTGATGTTTTGATTGGTAGAAGGGATAGCAACAAAAATGGTGTCTTTGTTCAAAGGCAGGTAATTCAACCTAGAGTAAAGAAGAGTGTGAGATTTGCAGAGAATGGTAATGTTTGTGAGGTTTATAGCACAAGAACTTGTGAATCGGATGGAAGTTCCTCAAATGATGACCAAGGCGAGGTTTTGAAGAATAGAAAGTTTGCAGTTGTAGATGTTGTGGCCACTTCTCAGGGTGTTGAAGATGACGAGGAAGTGCTGGTGGAGGACAGTGGAGGATCAACACGTAGTAGTGATGATGGAGATATAGTGAAAGAGCAGTTTCAAGCACACCAAGAGAAGATTTTGTTCTCTGCTCCATTGCCACTTAAAATGGAAAGCAGAACTGATTTGAAGAGTAAAGGCGTGAAAATTTTGACGTGA
Protein sequence

>Msa1139460

MASHHHYNHHHSHHQPPPPPPPPTHCYCTPSYPCYTPTPQPPHLSPQHDHLVQTISSILLSPQLNHLVHHNLVHDHLVHHPKSQTLHHQNHQSTLSSLLHRIETLESSLNQHQTHQSQSLRHSAARVIQTHFRSFLVRRSRTLRNLKQLASIKSSFIAIRSSFSTHTHFDFPALSLKAVNLLIKLDSIQDCDTMIVDGKRSISRDLVQFLDSIEEVAAKKHMHYVKAVKSSRSGQKVQRPRKPEGDDDEKKKLLKNLRGRVEKISKLCKVYGDDGEGLANEESIHDHDDDYDDDGVTDVLIGRRDSNKNGVFVQRQVIQPRVKKSVRFAENGNVCEVYSTRTCESDGSSSNDDQGEVLKNRKFAVVDVVATSQGVEDDEEVLVEDSGGSTRSSDDGDIVKEQFQAHQEKILFSAPLPLKMESRTDLKSKGVKILT*