AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1308850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1308850 MtrunA17_Chr8g0335341 95.610 729 25 2 1 728 1 723 0.0 1431
Msa1308850 MtrunA17_Chr7g0250681 68.738 531 127 7 227 728 323 843 0.0 707
Msa1308850 MtrunA17_Chr7g0250681 54.286 210 51 5 1 165 1 210 2.87e-48 183
Msa1308850 MtrunA17_Chr5g0405741 39.341 455 241 9 282 724 204 635 1.14e-92 302
Msa1308850 MtrunA17_Chr5g0405741 55.932 59 26 0 1 59 1 59 8.64e-12 68.6
Msa1308850 MtrunA17_Chr2g0303521 31.195 452 276 8 282 724 208 633 2.49e-52 192
Msa1308850 MtrunA17_Chr3g0139411 28.889 450 281 11 284 720 277 700 4.32e-52 192
Msa1308850 MtrunA17_Chr2g0331821 28.733 442 298 9 274 710 309 738 1.18e-48 183
Msa1308850 MtrunA17_Chr2g0331821 57.627 59 25 0 1 59 1 59 1.95e-14 77.4
Msa1308850 MtrunA17_Chr3g0124841 27.373 453 308 7 282 720 454 899 3.57e-44 171
Msa1308850 MtrunA17_Chr4g0004471 31.448 442 273 11 275 710 119 536 1.00e-43 166
Msa1308850 MtrunA17_Chr1g0211271 28.027 446 294 8 282 710 274 709 5.17e-42 163
Msa1308850 MtrunA17_Chr1g0154051 80.000 105 16 2 34 138 32 131 5.82e-40 143
Msa1308850 MtrunA17_Chr5g0448571 24.208 442 280 11 286 716 358 755 5.86e-27 117
Msa1308850 MtrunA17_Chr4g0072011 27.059 340 227 7 277 602 330 662 7.97e-27 116
Msa1308850 MtrunA17_Chr3g0143731 25.828 453 256 12 282 711 275 670 4.55e-26 114
Msa1308850 MtrunA17_Chr3g0130761 27.536 345 236 6 367 710 433 764 7.31e-25 110
Msa1308850 MtrunA17_Chr5g0440551 33.621 232 137 5 366 588 429 652 8.40e-25 110
Msa1308850 MtrunA17_Chr8g0341331 73.077 104 20 3 31 131 27 125 1.82e-24 99.8
Msa1308850 MtrunA17_Chr2g0298171 39.106 179 97 4 369 537 628 804 3.77e-24 108
Msa1308850 MtrunA17_Chr3g0118401 32.751 229 147 3 366 590 445 670 6.12e-23 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1308850 AT1G02110.1 57.296 747 254 17 1 727 1 702 0.0 795
Msa1308850 AT3G60320.1 56.212 813 254 18 1 728 1 796 0.0 776
Msa1308850 AT3G51290.3 40.889 450 241 9 282 727 200 628 2.26e-95 309
Msa1308850 AT3G51290.2 41.111 450 240 9 282 727 200 628 1.32e-93 309
Msa1308850 AT3G51290.1 40.444 450 237 10 282 727 200 622 1.28e-89 294
Msa1308850 AT2G34670.2 34.605 367 223 6 355 720 324 674 1.09e-60 217
Msa1308850 AT5G25590.1 29.605 456 276 9 283 720 330 758 4.77e-53 196
Msa1308850 AT1G52320.3 29.314 423 278 9 293 710 34 440 9.18e-51 184
Msa1308850 AT1G52320.4 29.314 423 278 9 293 710 34 440 9.18e-51 184
Msa1308850 AT1G52320.1 29.314 423 278 9 293 710 337 743 8.31e-50 187
Msa1308850 AT1G52320.2 29.314 423 278 9 293 710 360 766 1.53e-49 187
Msa1308850 AT1G52320.5 29.314 423 278 9 293 710 360 766 1.53e-49 187
Msa1308850 AT1G21740.1 28.667 450 305 8 282 720 497 941 3.25e-45 174
Msa1308850 AT1G77500.1 27.655 452 284 9 282 720 449 870 9.73e-40 157
Msa1308850 AT2G34670.1 33.628 226 142 4 355 580 324 541 2.74e-32 132
Msa1308850 AT4G35240.4 24.777 448 297 7 282 724 419 831 1.36e-29 126
Msa1308850 AT4G35240.3 24.777 448 297 7 282 724 419 831 1.36e-29 126
Msa1308850 AT4G35240.2 24.777 448 297 7 282 724 419 831 2.22e-29 125
Msa1308850 AT4G35240.1 24.777 448 297 7 282 724 419 831 2.22e-29 125
Msa1308850 AT2G17110.1 29.321 324 208 8 286 594 313 630 7.08e-29 123
Msa1308850 AT2G27090.2 33.621 232 144 6 366 592 425 651 1.09e-24 110
Msa1308850 AT2G27090.1 33.621 232 144 6 366 592 425 651 1.09e-24 110
Msa1308850 AT5G54480.1 25.862 406 244 11 247 626 270 644 8.20e-24 107
Msa1308850 AT1G20530.1 26.546 388 246 10 283 654 232 596 2.18e-23 105
Msa1308850 AT4G39790.1 31.696 224 146 5 369 588 375 595 2.50e-22 102
Msa1308850 AT4G30130.3 31.429 245 153 5 369 602 401 641 1.14e-21 100
Msa1308850 AT4G30130.1 31.429 245 153 5 369 602 401 641 1.14e-21 100
Msa1308850 AT2G19090.1 30.364 247 155 5 369 602 482 724 3.09e-21 99.8
Msa1308850 AT4G30130.4 43.210 81 45 1 369 449 401 480 1.03e-11 68.6
Msa1308850 AT4G30130.2 43.210 81 45 1 369 449 401 480 1.03e-11 68.6

Find 181 sgRNAs with CRISPR-Local

Find 304 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATAAATTACCTACTTTA+TGG 0.098163 8_4:-5207640 None:intergenic
TGCCGGAAATATTCGGAATT+TGG 0.192821 8_4:-5203800 None:intergenic
CTCCACTTTGGACCGGCTTT+TGG 0.216350 8_4:+5204629 Msa1308850:CDS
CAGCTGCCATCAATGGTCTT+AGG 0.218432 8_4:+5205626 Msa1308850:CDS
TCCCCTCTTATAGGAAATTA+AGG 0.220886 8_4:+5207793 Msa1308850:three_prime_UTR
CTCCAAATTCCGAATATTTC+CGG 0.231370 8_4:+5203798 Msa1308850:CDS
TCTGATGATGTATCGGATTC+TGG 0.247422 8_4:-5203857 None:intergenic
CCAATATCTTCCACAGAGTT+TGG 0.255194 8_4:-5207390 None:intergenic
GCTGTGCAACCCATTGTTTC+AGG 0.258271 8_4:-5203314 None:intergenic
GTCAAGAACTCAAGATTAAA+AGG 0.266491 8_4:+5207262 Msa1308850:CDS
CCAAACTCTGTGGAAGATAT+TGG 0.303854 8_4:+5207390 Msa1308850:CDS
ATGGGAAAACAAACTCTTTC+AGG 0.306558 8_4:+5204653 Msa1308850:CDS
AAACTTCTTCCGTTTGGAAC+TGG 0.310325 8_4:+5203750 Msa1308850:CDS
AGGTTTGCCTGGAATGTTTC+AGG 0.312214 8_4:+5207554 Msa1308850:CDS
CAGAGATGTTGCAGATTAGT+AGG 0.333806 8_4:+5204239 Msa1308850:CDS
AGAACGTTGCCGGAAATATT+CGG 0.340163 8_4:-5203807 None:intergenic
TCTCCTTAATTTCCTATAAG+AGG 0.341283 8_4:-5207796 None:intergenic
TCATATTCTATGGTTGGTAT+TGG 0.351215 8_4:+5207360 Msa1308850:CDS
CAATGTTGCTTTGCGTTTCA+TGG 0.352365 8_4:-5206882 None:intergenic
AAAGTGGAGCAGAGGCTCTT+TGG 0.352489 8_4:-5204615 None:intergenic
TGTTGCCGGCGGTGAGGTTC+CGG 0.353203 8_4:+5204096 Msa1308850:CDS
ATAATTAATCTGTTCTGCTT+TGG 0.354765 8_4:+5207658 Msa1308850:three_prime_UTR
CAGCAGCATTAGCAACAAAA+TGG 0.360224 8_4:+5207113 Msa1308850:CDS
TCACCGCCGGCAACAAATTC+CGG 0.362313 8_4:-5204088 None:intergenic
GCTTCCGAAGCGACAGTGTC+TGG 0.370101 8_4:-5207192 None:intergenic
CTTCTGAAACTTCTTCCGTT+TGG 0.372515 8_4:+5203744 Msa1308850:CDS
TTTGTGGTTGTTACGGTGGC+TGG 0.373103 8_4:-5203578 None:intergenic
AACACGTCGCCGGAAAATTC+CGG 0.375532 8_4:+5203619 Msa1308850:CDS
TGCTTTGCGTTTCATGGTAC+TGG 0.378095 8_4:-5206876 None:intergenic
CTGTTCTGCTTTGGCTTTCG+AGG 0.382977 8_4:+5207667 Msa1308850:three_prime_UTR
AAATAGAATCACAAAATTCT+TGG 0.384792 8_4:-5207770 None:intergenic
TTATAGGAAATTAAGGAGAT+TGG 0.393221 8_4:+5207800 Msa1308850:three_prime_UTR
AACTGCCAATGGTGGCTTTG+AGG 0.395022 8_4:-5204550 None:intergenic
TTTGATGCCGGTTCACTTGA+AGG 0.399037 8_4:+5204582 Msa1308850:CDS
TTAATCTGAAGGACTAGGTT+AGG 0.406705 8_4:-5203162 None:intergenic
TAGTGACTGTAACCTTGTTA+TGG 0.407255 8_4:-5205569 None:intergenic
AGGAGGTTCGGTGTAGTGAT+TGG 0.415100 8_4:+5203990 Msa1308850:CDS
GTCAATGCCTGAAACATTCC+AGG 0.419912 8_4:-5207561 None:intergenic
TCGGCGGTTGAGAACGTTGC+CGG 0.420345 8_4:-5203817 None:intergenic
AGCTGCCATCAATGGTCTTA+GGG 0.421008 8_4:+5205627 Msa1308850:CDS
CAGCTTGTTGAGCTTTGTCA+TGG 0.421587 8_4:+5205667 Msa1308850:CDS
AGTTGCCTGTTTGTGTGATT+CGG 0.424981 8_4:-5206956 None:intergenic
TGTTCTGCTTTGGCTTTCGA+GGG 0.426961 8_4:+5207668 Msa1308850:three_prime_UTR
GCCCGGTCCTTCAAGTGAAC+CGG 0.428595 8_4:-5204589 None:intergenic
AACTTCTTCCGTTTGGAACT+GGG 0.429013 8_4:+5203751 Msa1308850:CDS
CAGTTCGGAAACTCGCTTCT+CGG 0.429976 8_4:-5203686 None:intergenic
AGAGTCCCTAAGACCATTGA+TGG 0.436049 8_4:-5205632 None:intergenic
TAACCTTGTTATGGTAGCCT+TGG 0.439375 8_4:-5205560 None:intergenic
AACATCCGAATCACACAAAC+AGG 0.442893 8_4:+5206951 Msa1308850:CDS
AACATTGTACAGCAAGTTTG+TGG 0.443164 8_4:+5206898 Msa1308850:CDS
AAATCACCGGAATTTGTTGC+CGG 0.450095 8_4:+5204082 Msa1308850:CDS
AATCTACAGACAGGTTTGCC+TGG 0.453101 8_4:+5207543 Msa1308850:CDS
TTAGAACTTGGAACAATAAG+AGG 0.456298 8_4:-5207730 None:intergenic
GAAGGTGGAGAGTGCGGAAC+CGG 0.456738 8_4:-5203465 None:intergenic
ACGGTGGCTGGAGGAGGAGG+TGG 0.456906 8_4:-5203566 None:intergenic
ACAACAAAATAGTTAGAACT+TGG 0.457524 8_4:-5207742 None:intergenic
CATTGAACAAACGCTTGAAT+CGG 0.458131 8_4:-5203663 None:intergenic
GTTGTTACGGTGGCTGGAGG+AGG 0.459155 8_4:-5203572 None:intergenic
ATATATACCACCTGAAACAA+TGG 0.459754 8_4:+5203304 Msa1308850:exon
GTGGCTGGAGGAGGAGGTGG+CGG 0.461153 8_4:-5203563 None:intergenic
CACCATTGGCAGTTAAATAC+CGG 0.462465 8_4:+5204559 Msa1308850:CDS
CTCCTTAATTTCCTATAAGA+GGG 0.463804 8_4:-5207795 None:intergenic
TTGTTGTTGTCATTGTTGAC+TGG 0.464323 8_4:-5207090 None:intergenic
CGAGGAGGTGAATGAAGAAA+AGG 0.465133 8_4:+5204129 Msa1308850:CDS
GTTACGGTGGCTGGAGGAGG+AGG 0.465245 8_4:-5203569 None:intergenic
TATTCGGAATTTGGAGGAGA+AGG 0.467016 8_4:-5203791 None:intergenic
GGAGGTTCGGTGTAGTGATT+GGG 0.467726 8_4:+5203991 Msa1308850:CDS
TGCCGGTTCACTTGAAGGAC+CGG 0.468386 8_4:+5204587 Msa1308850:CDS
ACTAATTATTGTAACATCTC+AGG 0.470791 8_4:+5205588 Msa1308850:CDS
GTTAAATACCGGTTTGATGC+CGG 0.472409 8_4:+5204570 Msa1308850:CDS
TCGGAATTTGGAGGAGAAGG+AGG 0.477917 8_4:-5203788 None:intergenic
ACACGGACAAGTGGAGGAGC+CGG 0.482188 8_4:+5207869 Msa1308850:three_prime_UTR
GAAAACTGACACTGCTTACA+AGG 0.482447 8_4:+5207284 Msa1308850:CDS
TACAACTCATATTCTATGGT+TGG 0.486108 8_4:+5207354 Msa1308850:CDS
AAAGGTGAAGATGAAGCATA+AGG 0.487575 8_4:+5204147 Msa1308850:CDS
AAACGGAACGTGAGGAGGTT+CGG 0.488757 8_4:+5203978 Msa1308850:CDS
AAAGAGCCTCTGCTCCACTT+TGG 0.490480 8_4:+5204617 Msa1308850:CDS
TTTGAGAAAGCGGCGGTGGC+CGG 0.491434 8_4:+5204205 Msa1308850:CDS
GAATTTGGAGGAGAAGGAGG+AGG 0.496035 8_4:-5203785 None:intergenic
TGTTCCAGACACTGTCGCTT+CGG 0.496334 8_4:+5207188 Msa1308850:CDS
ACCTCCTCGTCTTTCCTCAC+CGG 0.497977 8_4:-5204115 None:intergenic
ACTACCTCAGCTGCCATCAA+TGG 0.500376 8_4:+5205619 Msa1308850:CDS
GAAGCATTCGAAGGCTATTG+AGG 0.506274 8_4:+5207497 Msa1308850:CDS
CGAGAAGCGAGTTTCCGAAC+TGG 0.509820 8_4:+5203687 Msa1308850:CDS
GCACTCTCCACCTTCGCCGC+CGG 0.510838 8_4:+5203473 Msa1308850:CDS
GTGTGAAGATTGAGCATGAA+AGG 0.511307 8_4:+5205473 Msa1308850:CDS
AGGTTCACCGGCGGCGAAGG+TGG 0.514217 8_4:-5203480 None:intergenic
AGAATGTGTGGAAGCTTCAC+CGG 0.514510 8_4:-5203638 None:intergenic
TAGTTTCCATCCATCACAAA+AGG 0.515874 8_4:-5207154 None:intergenic
CGGAAATATTCGGAATTTGG+AGG 0.517284 8_4:-5203797 None:intergenic
GAGAGGTTCACCGGCGGCGA+AGG 0.519230 8_4:-5203483 None:intergenic
TATATACCACCTGAAACAAT+GGG 0.521293 8_4:+5203305 Msa1308850:exon
CTTGTGAACCAATCATCAAA+AGG 0.523864 8_4:+5206922 Msa1308850:CDS
GGAACAAGAACTAGATCGAT+TGG 0.523973 8_4:+5203955 Msa1308850:CDS
AGCTTGTTGAGCTTTGTCAT+GGG 0.524450 8_4:+5205668 Msa1308850:CDS
TAGAATCAGCTCTGTCAGCT+TGG 0.525936 8_4:+5206986 Msa1308850:CDS
CAAGTGAACCGGCATCAAAC+CGG 0.530021 8_4:-5204578 None:intergenic
TGAGCAAAGTGAGCTCAACT+TGG 0.530284 8_4:+5204526 Msa1308850:CDS
AAGCGGCGGTGGCCGGAGAT+CGG 0.531676 8_4:+5204212 Msa1308850:CDS
CAGAGGCTCTTTGGACCGCC+CGG 0.533714 8_4:-5204606 None:intergenic
GGAAGATATTGGACAAGGCT+TGG 0.535265 8_4:+5207401 Msa1308850:CDS
AGAACTAGATCGATTGGAAA+CGG 0.539459 8_4:+5203961 Msa1308850:CDS
CACGGACAAGTGGAGGAGCC+GGG 0.540071 8_4:+5207870 Msa1308850:three_prime_UTR
TCAGAATCACAAGACTGAAA+CGG 0.540629 8_4:+5203934 Msa1308850:CDS
TCAACCGCCGAATCATAAAC+CGG 0.541451 8_4:+5203829 Msa1308850:CDS
AAGTGTTAATCTGAAGGACT+AGG 0.544611 8_4:-5203167 None:intergenic
AGAGATGTTGCAGATTAGTA+GGG 0.545879 8_4:+5204240 Msa1308850:CDS
ACACTTAATAATCTACAGAC+AGG 0.546583 8_4:+5207534 Msa1308850:CDS
GCCTCTGCTCCACTTTGGAC+CGG 0.546977 8_4:+5204622 Msa1308850:CDS
TTGCTTAATTAGGCTAGAGA+AGG 0.551085 8_4:+5205451 Msa1308850:intron
CATCTCTGATAACCGATCTC+CGG 0.555701 8_4:-5204224 None:intergenic
ATTGGAAACGGAACGTGAGG+AGG 0.556480 8_4:+5203973 Msa1308850:CDS
GAACGATTCTGATGATGTAT+CGG 0.557299 8_4:-5203864 None:intergenic
TTAGTAGGGCTCAACTTGAT+CGG 0.558121 8_4:+5204254 Msa1308850:CDS
AACTGCAAGTGTTAATCTGA+AGG 0.564018 8_4:-5203173 None:intergenic
GCGGTTGGAATGAACCAGTT+CGG 0.568073 8_4:-5203701 None:intergenic
GGAATTTGTTGCCGGCGGTG+AGG 0.568540 8_4:+5204090 Msa1308850:CDS
AAAATTCTCCCAGTTCCAAA+CGG 0.570472 8_4:-5203759 None:intergenic
CAAAAGGAAAATGCATCAGA+TGG 0.571615 8_4:-5207138 None:intergenic
TAAACTCGACAACGAAGAAA+CGG 0.572183 8_4:+5203340 Msa1308850:CDS
AACCGGTATTTAACTGCCAA+TGG 0.572575 8_4:-5204561 None:intergenic
CCGTTCATATGCCATAAAGT+AGG 0.573938 8_4:+5207629 Msa1308850:CDS
AGAACGCCGCCGTCTCATGA+AGG 0.575210 8_4:+5203376 Msa1308850:CDS
TTGGACCTCAAAGCCACCAT+TGG 0.576071 8_4:+5204545 Msa1308850:CDS
GGAGACAGCGAGAGGTTCAC+CGG 0.577083 8_4:-5203492 None:intergenic
CAGTGTCTGGAACACGGTCA+AGG 0.579288 8_4:-5207179 None:intergenic
TGCCAAAAGCCGGTCCAAAG+TGG 0.581464 8_4:-5204631 None:intergenic
CAAACTCTTTCAGGAAGTGA+AGG 0.582816 8_4:+5204662 Msa1308850:CDS
TCTGCAGAGTGAAGAATGCA+AGG 0.582873 8_4:+5205507 Msa1308850:CDS
TATCGCCGGTTTGAAGTCAT+CGG 0.584629 8_4:+5204057 Msa1308850:CDS
GATAGAGATGAAGTTATCGC+CGG 0.585630 8_4:+5204043 Msa1308850:CDS
TGTAACTCTCTTCGCCTCAC+CGG 0.588335 8_4:+5203446 Msa1308850:CDS
GCCGGTTCACTTGAAGGACC+GGG 0.588752 8_4:+5204588 Msa1308850:CDS
GCCGGTCCAAAGTGGAGCAG+AGG 0.591183 8_4:-5204623 None:intergenic
GGTTCGGTGTAGTGATTGGG+AGG 0.592402 8_4:+5203994 Msa1308850:CDS
CAGTTTCTGAAACACGTCGC+CGG 0.594202 8_4:+5203609 Msa1308850:CDS
AGTGTCTGGAACACGGTCAA+GGG 0.596369 8_4:-5207178 None:intergenic
GTGCAACCCATTGTTTCAGG+TGG 0.596711 8_4:-5203311 None:intergenic
GTGGTTGTTACGGTGGCTGG+AGG 0.605382 8_4:-5203575 None:intergenic
AAGACCATTGATGGCAGCTG+AGG 0.609697 8_4:-5205623 None:intergenic
GGCGGCGAAGGTGGAGAGTG+CGG 0.611743 8_4:-5203471 None:intergenic
CGGTATTTAACTGCCAATGG+TGG 0.612149 8_4:-5204558 None:intergenic
TCCTTAATTTCCTATAAGAG+GGG 0.612933 8_4:-5207794 None:intergenic
GACGTGTTTCAGAAACTGTA+CGG 0.616647 8_4:-5203604 None:intergenic
ACTGTGTTGATGAAAACGGC+GGG 0.618606 8_4:-5203524 None:intergenic
TGAAAACTGACACGGACAAG+TGG 0.619868 8_4:+5207860 Msa1308850:three_prime_UTR
GAAAACGGCGGGAATGTCGT+CGG 0.622356 8_4:-5203513 None:intergenic
TGGAATAATTCCAAACTCTG+TGG 0.623866 8_4:+5207380 Msa1308850:CDS
AACATAGAATCATTCACATG+TGG 0.629855 8_4:+5206842 Msa1308850:intron
TGAAAAGTTGAAGCATTCGA+AGG 0.634173 8_4:+5207488 Msa1308850:CDS
CTTGAATCGGAGAGAATGTG+TGG 0.636876 8_4:-5203650 None:intergenic
TCGATTGGAAACGGAACGTG+AGG 0.636903 8_4:+5203970 Msa1308850:CDS
CAGAGTGAAGAATGCAAGGG+AGG 0.640119 8_4:+5205511 Msa1308850:CDS
CCTACTTTATGGCATATGAA+CGG 0.642947 8_4:-5207629 None:intergenic
GTTCTGCTTTGGCTTTCGAG+GGG 0.645485 8_4:+5207669 Msa1308850:three_prime_UTR
AAACTGACACGGACAAGTGG+AGG 0.645665 8_4:+5207863 Msa1308850:three_prime_UTR
TGACAAAGCTCAACAAGCTG+AGG 0.646039 8_4:-5205664 None:intergenic
TGGAGAGTGCGGAACCGGTG+AGG 0.647266 8_4:-5203460 None:intergenic
AAACCAAGGCTACCATAACA+AGG 0.647826 8_4:+5205557 Msa1308850:CDS
TCACCGGAATTTGTTGCCGG+CGG 0.648273 8_4:+5204085 Msa1308850:CDS
AAGCGACAGTGTCTGGAACA+CGG 0.656033 8_4:-5207185 None:intergenic
CGCTCGCCACTACCTCGCGG+CGG 0.656448 8_4:+5203409 Msa1308850:CDS
ATTGCTTAACTGATATTACG+TGG 0.657821 8_4:-5207239 None:intergenic
CCTCACCGGAACCTCACCGC+CGG 0.660549 8_4:-5204101 None:intergenic
ATGTCGTCGGAGACAGCGAG+AGG 0.661397 8_4:-5203500 None:intergenic
TCGACAACGAAGAAACGGTA+CGG 0.665354 8_4:+5203345 Msa1308850:CDS
TACTGTGTTGATGAAAACGG+CGG 0.666428 8_4:-5203525 None:intergenic
CTGCAGAGTGAAGAATGCAA+GGG 0.667767 8_4:+5205508 Msa1308850:CDS
AACAAAGATAGACAAAACCA+AGG 0.673276 8_4:+5205543 Msa1308850:CDS
TCCGGTGAGGAAAGACGAGG+AGG 0.676721 8_4:+5204114 Msa1308850:CDS
CCGGCGGTGAGGTTCCGGTG+AGG 0.679781 8_4:+5204101 Msa1308850:CDS
AATCAGAGTGCGCCGCCGCG+AGG 0.683669 8_4:-5203421 None:intergenic
TCTGTGGAAGATATTGGACA+AGG 0.692232 8_4:+5207396 Msa1308850:CDS
AAACTGCTTCCTTCATGAGA+CGG 0.693412 8_4:-5203385 None:intergenic
ATAGTCTTTGAAAACTGACA+CGG 0.701263 8_4:+5207852 Msa1308850:three_prime_UTR
CTGCTTCCTTCATGAGACGG+CGG 0.729263 8_4:-5203382 None:intergenic
GACAGCGAGAGGTTCACCGG+CGG 0.729279 8_4:-5203489 None:intergenic
ACAACGAAGAAACGGTACGG+CGG 0.748653 8_4:+5203348 Msa1308850:CDS
TTACGCTCGCCACTACCTCG+CGG 0.753107 8_4:+5203406 Msa1308850:CDS
GGTTCCGGTGAGGAAAGACG+AGG 0.762077 8_4:+5204111 Msa1308850:CDS
GGTTCACTTGAAGGACCGGG+CGG 0.787079 8_4:+5204591 Msa1308850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATACTAATAATTGA+TGG + chr8_4:5204782-5204801 Msa1308850:intron 10.0%
!! TTAATCATATATTTAAATTG+GGG + chr8_4:5205797-5205816 Msa1308850:intron 10.0%
!!! TAATTTTTTGAACAATGTAA+AGG + chr8_4:5204733-5204752 Msa1308850:intron 15.0%
!! TAATCATATATTTAAATTGG+GGG + chr8_4:5205798-5205817 Msa1308850:intron 15.0%
!!! CAAATACTATTTCAAAATTT+TGG - chr8_4:5206000-5206019 None:intergenic 15.0%
!! AAATAGTATTTGAGAAAATA+TGG + chr8_4:5206008-5206027 Msa1308850:intron 15.0%
!! ATGAAATTGGAAAATTATAA+TGG + chr8_4:5206550-5206569 Msa1308850:intron 15.0%
!! TATTGAAATTTGAATTGTAA+GGG - chr8_4:5206627-5206646 None:intergenic 15.0%
!! ATATTGAAATTTGAATTGTA+AGG - chr8_4:5206628-5206647 None:intergenic 15.0%
!!! TATAAACTTGATAATCATAA+AGG + chr8_4:5206645-5206664 Msa1308850:intron 15.0%
!! ATTATAAATTACCTACTTTA+TGG - chr8_4:5207643-5207662 None:intergenic 15.0%
!! AGATTTATATCTGAAGAAAT+TGG + chr8_4:5204403-5204422 Msa1308850:intron 20.0%
!!! TAGTTTAGGTTGAATTAAAA+AGG - chr8_4:5205336-5205355 None:intergenic 20.0%
!!! ATTTAGAGTTAATTTGTGAA+GGG - chr8_4:5205381-5205400 None:intergenic 20.0%
!!! TATTTAGAGTTAATTTGTGA+AGG - chr8_4:5205382-5205401 None:intergenic 20.0%
!! TCTAAATACTCATCTAAATT+AGG + chr8_4:5205394-5205413 Msa1308850:intron 20.0%
!!! TTTTGCTTAATTGCTTAATT+AGG + chr8_4:5205441-5205460 Msa1308850:intron 20.0%
!!! ATTTTCTAGTAAAATGAGAT+TGG + chr8_4:5205863-5205882 Msa1308850:intron 20.0%
!!! ATACTATTTCAAAATTTTGG+TGG - chr8_4:5205997-5206016 None:intergenic 20.0%
!! ATTTGAGAGTTAATTATCAA+TGG + chr8_4:5206103-5206122 Msa1308850:intron 20.0%
!! TCTCAATACTAGTATATATA+TGG - chr8_4:5206683-5206702 None:intergenic 20.0%
!! AAATAGAATCACAAAATTCT+TGG - chr8_4:5207773-5207792 None:intergenic 20.0%
!!! TTTTACTGTGTTGATGAAAA+CGG - chr8_4:5203531-5203550 None:intergenic 25.0%
!! GGATTTAATGTTTTTCGTTA+TGG + chr8_4:5204424-5204443 Msa1308850:intron 25.0%
!! TACGATAAATTTTCATGTTG+AGG + chr8_4:5204885-5204904 Msa1308850:intron 25.0%
!!! TGATTCAATTTCGAGTTATA+AGG + chr8_4:5205121-5205140 Msa1308850:intron 25.0%
!!! TAAAAAGGAAGTGACTTTTT+TGG - chr8_4:5205321-5205340 None:intergenic 25.0%
! ACTAATTATTGTAACATCTC+AGG + chr8_4:5205588-5205607 Msa1308850:CDS 25.0%
! TGTGTTTGATATTCAATTCA+AGG + chr8_4:5205920-5205939 Msa1308850:intron 25.0%
! AACTTGATTCTACTGTATTA+TGG - chr8_4:5206150-5206169 None:intergenic 25.0%
! AGTGAACAAAAAATAACTTC+AGG - chr8_4:5206258-5206277 None:intergenic 25.0%
!!! GAAGTTATTTTTTGTTCACT+AGG + chr8_4:5206258-5206277 Msa1308850:intron 25.0%
!!! ATAAAATGGTTTGCTCAAAT+GGG - chr8_4:5206392-5206411 None:intergenic 25.0%
!!! AATAAAATGGTTTGCTCAAA+TGG - chr8_4:5206393-5206412 None:intergenic 25.0%
! GTAACTGTCAACAAATAAAA+TGG - chr8_4:5206406-5206425 None:intergenic 25.0%
!!! CTCTTTGAAATTTTATGATC+CGG - chr8_4:5207026-5207045 None:intergenic 25.0%
!! GTTTTACAACTCATATTCTA+TGG + chr8_4:5207350-5207369 Msa1308850:CDS 25.0%
! ATAATTAATCTGTTCTGCTT+TGG + chr8_4:5207658-5207677 Msa1308850:three_prime_UTR 25.0%
! ACAACAAAATAGTTAGAACT+TGG - chr8_4:5207745-5207764 None:intergenic 25.0%
! TTATAGGAAATTAAGGAGAT+TGG + chr8_4:5207800-5207819 Msa1308850:three_prime_UTR 25.0%
ATATATACCACCTGAAACAA+TGG + chr8_4:5203304-5203323 Msa1308850:exon 30.0%
TATATACCACCTGAAACAAT+GGG + chr8_4:5203305-5203324 Msa1308850:exon 30.0%
!! TATGAAGATGAATTTTCGAG+TGG - chr8_4:5203901-5203920 None:intergenic 30.0%
!! AAGAGAGAATTTTGAGAAAG+CGG + chr8_4:5204195-5204214 Msa1308850:CDS 30.0%
AACAATGTAAAGGTCTTCTA+GGG + chr8_4:5204743-5204762 Msa1308850:intron 30.0%
! TGCACATCATAATTTTGTTG+AGG + chr8_4:5205017-5205036 Msa1308850:intron 30.0%
CAACCTAAACTAATATGAAC+TGG + chr8_4:5205344-5205363 Msa1308850:intron 30.0%
! TTGCCAGTTCATATTAGTTT+AGG - chr8_4:5205350-5205369 None:intergenic 30.0%
AACAAAGATAGACAAAACCA+AGG + chr8_4:5205543-5205562 Msa1308850:CDS 30.0%
ATGTCTCTCTATTCTACTAT+TGG + chr8_4:5205692-5205711 Msa1308850:intron 30.0%
CTTATATCTAAGTTCAGAGA+GGG - chr8_4:5205719-5205738 None:intergenic 30.0%
ACTTATATCTAAGTTCAGAG+AGG - chr8_4:5205720-5205739 None:intergenic 30.0%
TATCTTATTCACATATGAGC+TGG + chr8_4:5205753-5205772 Msa1308850:intron 30.0%
GTATTTGCACAATGTATGAA+AGG + chr8_4:5205823-5205842 Msa1308850:intron 30.0%
! ATTGCTATTCGTACTTTCAT+TGG + chr8_4:5205970-5205989 Msa1308850:intron 30.0%
! TTGCTATTCGTACTTTCATT+GGG + chr8_4:5205971-5205990 Msa1308850:intron 30.0%
! AATACAGTAGAATCAAGTTG+TGG + chr8_4:5206151-5206170 Msa1308850:intron 30.0%
!!! TATTTTTTGTTCACTAGGTG+TGG + chr8_4:5206263-5206282 Msa1308850:intron 30.0%
!! TAAAATGGTTTGCTCAAATG+GGG - chr8_4:5206391-5206410 None:intergenic 30.0%
AGGGAAACAATTATACAGAA+TGG - chr8_4:5206507-5206526 None:intergenic 30.0%
CTGTATAATTGTTTCCCTTA+AGG + chr8_4:5206509-5206528 Msa1308850:intron 30.0%
CAATTTCATATACTATTGCC+AGG - chr8_4:5206539-5206558 None:intergenic 30.0%
CTGGCAATAGTATATGAAAT+TGG + chr8_4:5206537-5206556 Msa1308850:intron 30.0%
GAACATAGTAGCATATTTCT+TGG + chr8_4:5206572-5206591 Msa1308850:intron 30.0%
! ATTTCTTGGTAAAGCATCTT+TGG + chr8_4:5206586-5206605 Msa1308850:intron 30.0%
TTGATGCACAACAATAATGT+AGG - chr8_4:5206754-5206773 None:intergenic 30.0%
CATGTGAATGATTCTATGTT+TGG - chr8_4:5206842-5206861 None:intergenic 30.0%
AACATAGAATCATTCACATG+TGG + chr8_4:5206842-5206861 Msa1308850:intron 30.0%
!!! GACTTTATTTTGTCACTACA+TGG + chr8_4:5207045-5207064 Msa1308850:CDS 30.0%
!! TTATTTTGTCACTACATGGT+TGG + chr8_4:5207049-5207068 Msa1308850:CDS 30.0%
ATTGCTTAACTGATATTACG+TGG - chr8_4:5207242-5207261 None:intergenic 30.0%
GTCAAGAACTCAAGATTAAA+AGG + chr8_4:5207262-5207281 Msa1308850:CDS 30.0%
TACAACTCATATTCTATGGT+TGG + chr8_4:5207354-5207373 Msa1308850:CDS 30.0%
! TCATATTCTATGGTTGGTAT+TGG + chr8_4:5207360-5207379 Msa1308850:CDS 30.0%
ACACTTAATAATCTACAGAC+AGG + chr8_4:5207534-5207553 Msa1308850:CDS 30.0%
!!! GTATAAATGCCGATTTTTTC+AGG + chr8_4:5207702-5207721 Msa1308850:three_prime_UTR 30.0%
TTAGAACTTGGAACAATAAG+AGG - chr8_4:5207733-5207752 None:intergenic 30.0%
! ATTCTATTTTCCCCTCTTAT+AGG + chr8_4:5207784-5207803 Msa1308850:three_prime_UTR 30.0%
TCCTTAATTTCCTATAAGAG+GGG - chr8_4:5207797-5207816 None:intergenic 30.0%
CTCCTTAATTTCCTATAAGA+GGG - chr8_4:5207798-5207817 None:intergenic 30.0%
TCTCCTTAATTTCCTATAAG+AGG - chr8_4:5207799-5207818 None:intergenic 30.0%
! ATAGTCTTTGAAAACTGACA+CGG + chr8_4:5207852-5207871 Msa1308850:three_prime_UTR 30.0%
TTAATCTGAAGGACTAGGTT+AGG - chr8_4:5203165-5203184 None:intergenic 35.0%
AAGTGTTAATCTGAAGGACT+AGG - chr8_4:5203170-5203189 None:intergenic 35.0%
AACTGCAAGTGTTAATCTGA+AGG - chr8_4:5203176-5203195 None:intergenic 35.0%
TAAACTCGACAACGAAGAAA+CGG + chr8_4:5203340-5203359 Msa1308850:CDS 35.0%
CATTGAACAAACGCTTGAAT+CGG - chr8_4:5203666-5203685 None:intergenic 35.0%
!!! TGTTGATTCATTTTGATGCG+CGG - chr8_4:5203723-5203742 None:intergenic 35.0%
AAAATTCTCCCAGTTCCAAA+CGG - chr8_4:5203762-5203781 None:intergenic 35.0%
CTCCAAATTCCGAATATTTC+CGG + chr8_4:5203798-5203817 Msa1308850:CDS 35.0%
! GGATTTTCCGGTTTATGATT+CGG - chr8_4:5203839-5203858 None:intergenic 35.0%
! GAACGATTCTGATGATGTAT+CGG - chr8_4:5203867-5203886 None:intergenic 35.0%
TCAGAATCACAAGACTGAAA+CGG + chr8_4:5203934-5203953 Msa1308850:CDS 35.0%
AGAACTAGATCGATTGGAAA+CGG + chr8_4:5203961-5203980 Msa1308850:CDS 35.0%
! TTTTTCCGATGACTTCAAAC+CGG - chr8_4:5204065-5204084 None:intergenic 35.0%
AAAGGTGAAGATGAAGCATA+AGG + chr8_4:5204147-5204166 Msa1308850:CDS 35.0%
AGAGATGTTGCAGATTAGTA+GGG + chr8_4:5204240-5204259 Msa1308850:CDS 35.0%
ATGGGAAAACAAACTCTTTC+AGG + chr8_4:5204653-5204672 Msa1308850:CDS 35.0%
GAACAATGTAAAGGTCTTCT+AGG + chr8_4:5204742-5204761 Msa1308850:intron 35.0%
ACAATGTAAAGGTCTTCTAG+GGG + chr8_4:5204744-5204763 Msa1308850:intron 35.0%
! ATCACAGAATCAGTCACTTT+CGG + chr8_4:5204819-5204838 Msa1308850:intron 35.0%
AACACTAAAACACCTAAAGC+AGG - chr8_4:5204930-5204949 None:intergenic 35.0%
CAGTGAGTTAAGCAAAATCA+CGG - chr8_4:5205080-5205099 None:intergenic 35.0%
TTATAAGGAATGAGTCGAGT+AGG + chr8_4:5205136-5205155 Msa1308850:intron 35.0%
TATAAGGAATGAGTCGAGTA+GGG + chr8_4:5205137-5205156 Msa1308850:intron 35.0%
TTGCTTAATTAGGCTAGAGA+AGG + chr8_4:5205451-5205470 Msa1308850:intron 35.0%
TAGTGACTGTAACCTTGTTA+TGG - chr8_4:5205572-5205591 None:intergenic 35.0%
! TTCCTGACTTTGTTGACTAA+TGG - chr8_4:5205890-5205909 None:intergenic 35.0%
CTACTGTATTATGGCAATAC+AGG - chr8_4:5206141-5206160 None:intergenic 35.0%
TTACTCTTGCACACATAGTT+AGG - chr8_4:5206204-5206223 None:intergenic 35.0%
AACATTGTACAGCAAGTTTG+TGG + chr8_4:5206898-5206917 Msa1308850:CDS 35.0%
!! CTTTTGATGATTGGTTCACA+AGG - chr8_4:5206924-5206943 None:intergenic 35.0%
CTTGTGAACCAATCATCAAA+AGG + chr8_4:5206922-5206941 Msa1308850:CDS 35.0%
!! TTGAGTGACCTTTTGATGAT+TGG - chr8_4:5206933-5206952 None:intergenic 35.0%
!! CTTGGCATTCAAGTTTTATC+CGG + chr8_4:5207004-5207023 Msa1308850:CDS 35.0%
! TTGTTGTTGTCATTGTTGAC+TGG - chr8_4:5207093-5207112 None:intergenic 35.0%
CAAAAGGAAAATGCATCAGA+TGG - chr8_4:5207141-5207160 None:intergenic 35.0%
! GATGCATTTTCCTTTTGTGA+TGG + chr8_4:5207144-5207163 Msa1308850:CDS 35.0%
!! CATTTTCCTTTTGTGATGGA+TGG + chr8_4:5207148-5207167 Msa1308850:CDS 35.0%
TAGTTTCCATCCATCACAAA+AGG - chr8_4:5207157-5207176 None:intergenic 35.0%
TGGAATAATTCCAAACTCTG+TGG + chr8_4:5207380-5207399 Msa1308850:CDS 35.0%
!!! CCAATTTTTTCTCAGCAAGA+GGG - chr8_4:5207437-5207456 None:intergenic 35.0%
!!! TCCAATTTTTTCTCAGCAAG+AGG - chr8_4:5207438-5207457 None:intergenic 35.0%
! TGAAAAGTTGAAGCATTCGA+AGG + chr8_4:5207488-5207507 Msa1308850:CDS 35.0%
CCTACTTTATGGCATATGAA+CGG - chr8_4:5207632-5207651 None:intergenic 35.0%
TCCCCTCTTATAGGAAATTA+AGG + chr8_4:5207793-5207812 Msa1308850:three_prime_UTR 35.0%
AAACTGCTTCCTTCATGAGA+CGG - chr8_4:5203388-5203407 None:intergenic 40.0%
! TACTGTGTTGATGAAAACGG+CGG - chr8_4:5203528-5203547 None:intergenic 40.0%
!! ACGGAGTTTTGTGGTTGTTA+CGG - chr8_4:5203588-5203607 None:intergenic 40.0%
! AGAAACTGTACGGAGTTTTG+TGG - chr8_4:5203597-5203616 None:intergenic 40.0%
GACGTGTTTCAGAAACTGTA+CGG - chr8_4:5203607-5203626 None:intergenic 40.0%
!! CTTCTGAAACTTCTTCCGTT+TGG + chr8_4:5203744-5203763 Msa1308850:CDS 40.0%
! AAACTTCTTCCGTTTGGAAC+TGG + chr8_4:5203750-5203769 Msa1308850:CDS 40.0%
! AACTTCTTCCGTTTGGAACT+GGG + chr8_4:5203751-5203770 Msa1308850:CDS 40.0%
TATTCGGAATTTGGAGGAGA+AGG - chr8_4:5203794-5203813 None:intergenic 40.0%
CGGAAATATTCGGAATTTGG+AGG - chr8_4:5203800-5203819 None:intergenic 40.0%
TGCCGGAAATATTCGGAATT+TGG - chr8_4:5203803-5203822 None:intergenic 40.0%
AGAACGTTGCCGGAAATATT+CGG - chr8_4:5203810-5203829 None:intergenic 40.0%
!! TTTTCCGGTTTATGATTCGG+CGG - chr8_4:5203836-5203855 None:intergenic 40.0%
! GTATCGGATTCTGGATTTTC+CGG - chr8_4:5203851-5203870 None:intergenic 40.0%
! TCTGATGATGTATCGGATTC+TGG - chr8_4:5203860-5203879 None:intergenic 40.0%
GGAACAAGAACTAGATCGAT+TGG + chr8_4:5203955-5203974 Msa1308850:CDS 40.0%
GATAGAGATGAAGTTATCGC+CGG + chr8_4:5204043-5204062 Msa1308850:CDS 40.0%
GAAGTCATCGGAAAAATCAC+CGG + chr8_4:5204069-5204088 Msa1308850:CDS 40.0%
!! AAATCACCGGAATTTGTTGC+CGG + chr8_4:5204082-5204101 Msa1308850:CDS 40.0%
!! AGAGAATTTTGAGAAAGCGG+CGG + chr8_4:5204198-5204217 Msa1308850:CDS 40.0%
CAGAGATGTTGCAGATTAGT+AGG + chr8_4:5204239-5204258 Msa1308850:CDS 40.0%
TTAGTAGGGCTCAACTTGAT+CGG + chr8_4:5204254-5204273 Msa1308850:CDS 40.0%
GTTTGCTTACTGAGAAAGTG+CGG + chr8_4:5204324-5204343 Msa1308850:intron 40.0%
! TTTGCTTACTGAGAAAGTGC+GGG + chr8_4:5204325-5204344 Msa1308850:intron 40.0%
CACCATTGGCAGTTAAATAC+CGG + chr8_4:5204559-5204578 Msa1308850:CDS 40.0%
AACCGGTATTTAACTGCCAA+TGG - chr8_4:5204564-5204583 None:intergenic 40.0%
!! GTTAAATACCGGTTTGATGC+CGG + chr8_4:5204570-5204589 Msa1308850:CDS 40.0%
CAAACTCTTTCAGGAAGTGA+AGG + chr8_4:5204662-5204681 Msa1308850:CDS 40.0%
! CTGATGATGTTTCCTGCTTT+AGG + chr8_4:5204915-5204934 Msa1308850:intron 40.0%
CTTAACTCACTGCGAAACAA+GGG + chr8_4:5205088-5205107 Msa1308850:intron 40.0%
CATGCTAATCAAACCACGTT+TGG - chr8_4:5205206-5205225 None:intergenic 40.0%
CGTGGTTTGATTAGCATGTT+TGG + chr8_4:5205208-5205227 Msa1308850:intron 40.0%
GTGTGAAGATTGAGCATGAA+AGG + chr8_4:5205473-5205492 Msa1308850:CDS 40.0%
AAACCAAGGCTACCATAACA+AGG + chr8_4:5205557-5205576 Msa1308850:CDS 40.0%
! TAACCTTGTTATGGTAGCCT+TGG - chr8_4:5205563-5205582 None:intergenic 40.0%
!! AGCTTGTTGAGCTTTGTCAT+GGG + chr8_4:5205668-5205687 Msa1308850:CDS 40.0%
GACCATTAGTCAACAAAGTC+AGG + chr8_4:5205885-5205904 Msa1308850:intron 40.0%
GTCAACAAAGTCAGGAACTT+AGG + chr8_4:5205893-5205912 Msa1308850:intron 40.0%
GAGCAACATTGAGTCTGAAT+TGG - chr8_4:5206043-5206062 None:intergenic 40.0%
! TATTGCCAGGTTGACCTTAA+GGG - chr8_4:5206526-5206545 None:intergenic 40.0%
!! CAATGTTGCTTTGCGTTTCA+TGG - chr8_4:5206885-5206904 None:intergenic 40.0%
AACATCCGAATCACACAAAC+AGG + chr8_4:5206951-5206970 Msa1308850:CDS 40.0%
AGTTGCCTGTTTGTGTGATT+CGG - chr8_4:5206959-5206978 None:intergenic 40.0%
CAGCAGCATTAGCAACAAAA+TGG + chr8_4:5207113-5207132 Msa1308850:CDS 40.0%
GAAAACTGACACTGCTTACA+AGG + chr8_4:5207284-5207303 Msa1308850:CDS 40.0%
CCAATATCTTCCACAGAGTT+TGG - chr8_4:5207393-5207412 None:intergenic 40.0%
CCAAACTCTGTGGAAGATAT+TGG + chr8_4:5207390-5207409 Msa1308850:CDS 40.0%
TCTGTGGAAGATATTGGACA+AGG + chr8_4:5207396-5207415 Msa1308850:CDS 40.0%
CCCTCTTGCTGAGAAAAAAT+TGG + chr8_4:5207434-5207453 Msa1308850:CDS 40.0%
CCGTTCATATGCCATAAAGT+AGG + chr8_4:5207629-5207648 Msa1308850:CDS 40.0%
TCGACAACGAAGAAACGGTA+CGG + chr8_4:5203345-5203364 Msa1308850:CDS 45.0%
! ACTGTGTTGATGAAAACGGC+GGG - chr8_4:5203527-5203546 None:intergenic 45.0%
!! GAGTTTTGTGGTTGTTACGG+TGG - chr8_4:5203585-5203604 None:intergenic 45.0%
! GAAGCTTCACCGGAATTTTC+CGG - chr8_4:5203631-5203650 None:intergenic 45.0%
AGAATGTGTGGAAGCTTCAC+CGG - chr8_4:5203641-5203660 None:intergenic 45.0%
CTTGAATCGGAGAGAATGTG+TGG - chr8_4:5203653-5203672 None:intergenic 45.0%
!! GATTCATTTTGATGCGCGGT+TGG - chr8_4:5203719-5203738 None:intergenic 45.0%
TCAACCGCCGAATCATAAAC+CGG + chr8_4:5203829-5203848 Msa1308850:CDS 45.0%
!! TATCGCCGGTTTGAAGTCAT+CGG + chr8_4:5204057-5204076 Msa1308850:CDS 45.0%
CGAGGAGGTGAATGAAGAAA+AGG + chr8_4:5204129-5204148 Msa1308850:CDS 45.0%
CATCTCTGATAACCGATCTC+CGG - chr8_4:5204227-5204246 None:intergenic 45.0%
TGAGCAAAGTGAGCTCAACT+TGG + chr8_4:5204526-5204545 Msa1308850:CDS 45.0%
CGGTATTTAACTGCCAATGG+TGG - chr8_4:5204561-5204580 None:intergenic 45.0%
!! TTTGATGCCGGTTCACTTGA+AGG + chr8_4:5204582-5204601 Msa1308850:CDS 45.0%
! TGTTTTCCCATGCCAAAAGC+CGG - chr8_4:5204644-5204663 None:intergenic 45.0%
! TCTTCTAGGGGTAGTGTAGT+GGG + chr8_4:5204756-5204775 Msa1308850:intron 45.0%
GCAGGAAACATCATCAGCAA+AGG - chr8_4:5204912-5204931 None:intergenic 45.0%
GCTTAACTCACTGCGAAACA+AGG + chr8_4:5205087-5205106 Msa1308850:intron 45.0%
AGGGTGAGTGGATGGATAAT+AGG + chr8_4:5205156-5205175 Msa1308850:intron 45.0%
ACGTTCAAATGCACCAAACG+TGG + chr8_4:5205190-5205209 Msa1308850:intron 45.0%
TCTGCAGAGTGAAGAATGCA+AGG + chr8_4:5205507-5205526 Msa1308850:CDS 45.0%
CTGCAGAGTGAAGAATGCAA+GGG + chr8_4:5205508-5205527 Msa1308850:CDS 45.0%
! AGCTGCCATCAATGGTCTTA+GGG + chr8_4:5205627-5205646 Msa1308850:CDS 45.0%
AGAGTCCCTAAGACCATTGA+TGG - chr8_4:5205635-5205654 None:intergenic 45.0%
TGACAAAGCTCAACAAGCTG+AGG - chr8_4:5205667-5205686 None:intergenic 45.0%
!! CAGCTTGTTGAGCTTTGTCA+TGG + chr8_4:5205667-5205686 Msa1308850:CDS 45.0%
CACTAGGTGTGGTGTATGAA+TGG + chr8_4:5206274-5206293 Msa1308850:intron 45.0%
CAGCGAGTTCTGCGAAATTA+CGG + chr8_4:5206302-5206321 Msa1308850:intron 45.0%
TGTTTCCCTTAAGGTCAACC+TGG + chr8_4:5206518-5206537 Msa1308850:intron 45.0%
! CTATTGCCAGGTTGACCTTA+AGG - chr8_4:5206527-5206546 None:intergenic 45.0%
ATTAACTGTCGTTGCTGCAC+AGG - chr8_4:5206707-5206726 None:intergenic 45.0%
TGTGCAGCAACGACAGTTAA+TGG + chr8_4:5206706-5206725 Msa1308850:intron 45.0%
TGCTTTGCGTTTCATGGTAC+TGG - chr8_4:5206879-5206898 None:intergenic 45.0%
TAGAATCAGCTCTGTCAGCT+TGG + chr8_4:5206986-5207005 Msa1308850:CDS 45.0%
GGAAGATATTGGACAAGGCT+TGG + chr8_4:5207401-5207420 Msa1308850:CDS 45.0%
! GAAGCATTCGAAGGCTATTG+AGG + chr8_4:5207497-5207516 Msa1308850:CDS 45.0%
AATCTACAGACAGGTTTGCC+TGG + chr8_4:5207543-5207562 Msa1308850:CDS 45.0%
AGGTTTGCCTGGAATGTTTC+AGG + chr8_4:5207554-5207573 Msa1308850:CDS 45.0%
GTCAATGCCTGAAACATTCC+AGG - chr8_4:5207564-5207583 None:intergenic 45.0%
!! TGTTCTGCTTTGGCTTTCGA+GGG + chr8_4:5207668-5207687 Msa1308850:three_prime_UTR 45.0%
GAGGCAGTGCCTGAAAAAAT+CGG - chr8_4:5207714-5207733 None:intergenic 45.0%
TGAAAACTGACACGGACAAG+TGG + chr8_4:5207860-5207879 Msa1308850:three_prime_UTR 45.0%
!!! TTTTAATCATATATTTAAAT+TGG + chr8_4:5205795-5205814 Msa1308850:intron 5.0%
!! TTTAATCATATATTTAAATT+GGG + chr8_4:5205796-5205815 Msa1308850:intron 5.0%
GTGCAACCCATTGTTTCAGG+TGG - chr8_4:5203314-5203333 None:intergenic 50.0%
GCTGTGCAACCCATTGTTTC+AGG - chr8_4:5203317-5203336 None:intergenic 50.0%
ACAACGAAGAAACGGTACGG+CGG + chr8_4:5203348-5203367 Msa1308850:CDS 50.0%
TGTAACTCTCTTCGCCTCAC+CGG + chr8_4:5203446-5203465 Msa1308850:CDS 50.0%
TTTGTGGTTGTTACGGTGGC+TGG - chr8_4:5203581-5203600 None:intergenic 50.0%
!! CAGTTTCTGAAACACGTCGC+CGG + chr8_4:5203609-5203628 Msa1308850:CDS 50.0%
AACACGTCGCCGGAAAATTC+CGG + chr8_4:5203619-5203638 Msa1308850:CDS 50.0%
CAGTTCGGAAACTCGCTTCT+CGG - chr8_4:5203689-5203708 None:intergenic 50.0%
GCGGTTGGAATGAACCAGTT+CGG - chr8_4:5203704-5203723 None:intergenic 50.0%
GAATTTGGAGGAGAAGGAGG+AGG - chr8_4:5203788-5203807 None:intergenic 50.0%
TCGGAATTTGGAGGAGAAGG+AGG - chr8_4:5203791-5203810 None:intergenic 50.0%
TCGATTGGAAACGGAACGTG+AGG + chr8_4:5203970-5203989 Msa1308850:CDS 50.0%
ATTGGAAACGGAACGTGAGG+AGG + chr8_4:5203973-5203992 Msa1308850:CDS 50.0%
AAACGGAACGTGAGGAGGTT+CGG + chr8_4:5203978-5203997 Msa1308850:CDS 50.0%
!! AGGAGGTTCGGTGTAGTGAT+TGG + chr8_4:5203990-5204009 Msa1308850:CDS 50.0%
!! GGAGGTTCGGTGTAGTGATT+GGG + chr8_4:5203991-5204010 Msa1308850:CDS 50.0%
!! GAATTTTGAGAAAGCGGCGG+TGG + chr8_4:5204201-5204220 Msa1308850:CDS 50.0%
TTGGACCTCAAAGCCACCAT+TGG + chr8_4:5204545-5204564 Msa1308850:CDS 50.0%
! AACTGCCAATGGTGGCTTTG+AGG - chr8_4:5204553-5204572 None:intergenic 50.0%
CAAGTGAACCGGCATCAAAC+CGG - chr8_4:5204581-5204600 None:intergenic 50.0%
AAAGTGGAGCAGAGGCTCTT+TGG - chr8_4:5204618-5204637 None:intergenic 50.0%
AAAGAGCCTCTGCTCCACTT+TGG + chr8_4:5204617-5204636 Msa1308850:CDS 50.0%
!! TTTGGACCGGCTTTTGGCAT+GGG + chr8_4:5204635-5204654 Msa1308850:CDS 50.0%
! GTCTTCTAGGGGTAGTGTAG+TGG + chr8_4:5204755-5204774 Msa1308850:intron 50.0%
AATGAGTCGAGTAGGGTGAG+TGG + chr8_4:5205144-5205163 Msa1308850:intron 50.0%
CAGAGTGAAGAATGCAAGGG+AGG + chr8_4:5205511-5205530 Msa1308850:CDS 50.0%
ACTACCTCAGCTGCCATCAA+TGG + chr8_4:5205619-5205638 Msa1308850:CDS 50.0%
! AAGACCATTGATGGCAGCTG+AGG - chr8_4:5205626-5205645 None:intergenic 50.0%
CAGCTGCCATCAATGGTCTT+AGG + chr8_4:5205626-5205645 Msa1308850:CDS 50.0%
! AGTGTCTGGAACACGGTCAA+GGG - chr8_4:5207181-5207200 None:intergenic 50.0%
! AAGCGACAGTGTCTGGAACA+CGG - chr8_4:5207188-5207207 None:intergenic 50.0%
! TGTTCCAGACACTGTCGCTT+CGG + chr8_4:5207188-5207207 Msa1308850:CDS 50.0%
!! CTGTTCTGCTTTGGCTTTCG+AGG + chr8_4:5207667-5207686 Msa1308850:three_prime_UTR 50.0%
!! GTTCTGCTTTGGCTTTCGAG+GGG + chr8_4:5207669-5207688 Msa1308850:three_prime_UTR 50.0%
AAACTGACACGGACAAGTGG+AGG + chr8_4:5207863-5207882 Msa1308850:three_prime_UTR 50.0%
CTGCTTCCTTCATGAGACGG+CGG - chr8_4:5203385-5203404 None:intergenic 55.0%
GAAAACGGCGGGAATGTCGT+CGG - chr8_4:5203516-5203535 None:intergenic 55.0%
CGAGAAGCGAGTTTCCGAAC+TGG + chr8_4:5203687-5203706 Msa1308850:CDS 55.0%
!! GGTTCGGTGTAGTGATTGGG+AGG + chr8_4:5203994-5204013 Msa1308850:CDS 55.0%
!! TCACCGGAATTTGTTGCCGG+CGG + chr8_4:5204085-5204104 Msa1308850:CDS 55.0%
TCACCGCCGGCAACAAATTC+CGG - chr8_4:5204091-5204110 None:intergenic 55.0%
ACCTCCTCGTCTTTCCTCAC+CGG - chr8_4:5204118-5204137 None:intergenic 55.0%
!! TGCCGGTTCACTTGAAGGAC+CGG + chr8_4:5204587-5204606 Msa1308850:CDS 55.0%
!! CTCCACTTTGGACCGGCTTT+TGG + chr8_4:5204629-5204648 Msa1308850:CDS 55.0%
TGCCAAAAGCCGGTCCAAAG+TGG - chr8_4:5204634-5204653 None:intergenic 55.0%
!! CTTTGGACCGGCTTTTGGCA+TGG + chr8_4:5204634-5204653 Msa1308850:CDS 55.0%
AGTCGAGTAGGGTGAGTGGA+TGG + chr8_4:5205148-5205167 Msa1308850:intron 55.0%
! CAGTGTCTGGAACACGGTCA+AGG - chr8_4:5207182-5207201 None:intergenic 55.0%
AGAACGCCGCCGTCTCATGA+AGG + chr8_4:5203376-5203395 Msa1308850:CDS 60.0%
TTACGCTCGCCACTACCTCG+CGG + chr8_4:5203406-5203425 Msa1308850:CDS 60.0%
! GAAGGTGGAGAGTGCGGAAC+CGG - chr8_4:5203468-5203487 None:intergenic 60.0%
GGAGACAGCGAGAGGTTCAC+CGG - chr8_4:5203495-5203514 None:intergenic 60.0%
ATGTCGTCGGAGACAGCGAG+AGG - chr8_4:5203503-5203522 None:intergenic 60.0%
GTTGTTACGGTGGCTGGAGG+AGG - chr8_4:5203575-5203594 None:intergenic 60.0%
GTGGTTGTTACGGTGGCTGG+AGG - chr8_4:5203578-5203597 None:intergenic 60.0%
TCGGCGGTTGAGAACGTTGC+CGG - chr8_4:5203820-5203839 None:intergenic 60.0%
!! GGAATTTGTTGCCGGCGGTG+AGG + chr8_4:5204090-5204109 Msa1308850:CDS 60.0%
GGTTCCGGTGAGGAAAGACG+AGG + chr8_4:5204111-5204130 Msa1308850:CDS 60.0%
TCCGGTGAGGAAAGACGAGG+AGG + chr8_4:5204114-5204133 Msa1308850:CDS 60.0%
TTTGAGAAAGCGGCGGTGGC+CGG + chr8_4:5204205-5204224 Msa1308850:CDS 60.0%
! GCCGGTTCACTTGAAGGACC+GGG + chr8_4:5204588-5204607 Msa1308850:CDS 60.0%
GCCCGGTCCTTCAAGTGAAC+CGG - chr8_4:5204592-5204611 None:intergenic 60.0%
GGTTCACTTGAAGGACCGGG+CGG + chr8_4:5204591-5204610 Msa1308850:CDS 60.0%
GCCTCTGCTCCACTTTGGAC+CGG + chr8_4:5204622-5204641 Msa1308850:CDS 60.0%
GCTTCCGAAGCGACAGTGTC+TGG - chr8_4:5207195-5207214 None:intergenic 60.0%
ACACGGACAAGTGGAGGAGC+CGG + chr8_4:5207869-5207888 Msa1308850:three_prime_UTR 60.0%
! TGGAGAGTGCGGAACCGGTG+AGG - chr8_4:5203463-5203482 None:intergenic 65.0%
GACAGCGAGAGGTTCACCGG+CGG - chr8_4:5203492-5203511 None:intergenic 65.0%
GTTACGGTGGCTGGAGGAGG+AGG - chr8_4:5203572-5203591 None:intergenic 65.0%
TGTTGCCGGCGGTGAGGTTC+CGG + chr8_4:5204096-5204115 Msa1308850:CDS 65.0%
CAGAGGCTCTTTGGACCGCC+CGG - chr8_4:5204609-5204628 None:intergenic 65.0%
GCCGGTCCAAAGTGGAGCAG+AGG - chr8_4:5204626-5204645 None:intergenic 65.0%
AATCAGAGTGCGCCGCCGCG+AGG - chr8_4:5203424-5203443 None:intergenic 70.0%
GGCGGCGAAGGTGGAGAGTG+CGG - chr8_4:5203474-5203493 None:intergenic 70.0%
GCACTCTCCACCTTCGCCGC+CGG + chr8_4:5203473-5203492 Msa1308850:CDS 70.0%
AGGTTCACCGGCGGCGAAGG+TGG - chr8_4:5203483-5203502 None:intergenic 70.0%
GAGAGGTTCACCGGCGGCGA+AGG - chr8_4:5203486-5203505 None:intergenic 70.0%
GTGGCTGGAGGAGGAGGTGG+CGG - chr8_4:5203566-5203585 None:intergenic 70.0%
ACGGTGGCTGGAGGAGGAGG+TGG - chr8_4:5203569-5203588 None:intergenic 70.0%
CCTCACCGGAACCTCACCGC+CGG - chr8_4:5204104-5204123 None:intergenic 70.0%
AAGCGGCGGTGGCCGGAGAT+CGG + chr8_4:5204212-5204231 Msa1308850:CDS 70.0%
CGCTCGCCACTACCTCGCGG+CGG + chr8_4:5203409-5203428 Msa1308850:CDS 75.0%
AGTGCGCCGCCGCGAGGTAG+TGG - chr8_4:5203418-5203437 None:intergenic 75.0%
CCGGCGGTGAGGTTCCGGTG+AGG + chr8_4:5204101-5204120 Msa1308850:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 5203147 5207892 5203147 ID=Msa1308850;Name=Msa1308850
chr8_4 mRNA 5203147 5207892 5203147 ID=Msa1308850-mRNA-1;Parent=Msa1308850;Name=Msa1308850-mRNA-1;_AED=0.03;_eAED=0.03;_QI=176|1|1|1|1|1|4|242|728
chr8_4 exon 5203147 5204295 5203147 ID=Msa1308850-mRNA-1:exon:1619;Parent=Msa1308850-mRNA-1
chr8_4 exon 5204499 5204683 5204499 ID=Msa1308850-mRNA-1:exon:1620;Parent=Msa1308850-mRNA-1
chr8_4 exon 5205463 5205689 5205463 ID=Msa1308850-mRNA-1:exon:1621;Parent=Msa1308850-mRNA-1
chr8_4 exon 5206849 5207892 5206849 ID=Msa1308850-mRNA-1:exon:1622;Parent=Msa1308850-mRNA-1
chr8_4 five_prime_UTR 5203147 5203322 5203147 ID=Msa1308850-mRNA-1:five_prime_utr;Parent=Msa1308850-mRNA-1
chr8_4 CDS 5203323 5204295 5203323 ID=Msa1308850-mRNA-1:cds;Parent=Msa1308850-mRNA-1
chr8_4 CDS 5204499 5204683 5204499 ID=Msa1308850-mRNA-1:cds;Parent=Msa1308850-mRNA-1
chr8_4 CDS 5205463 5205689 5205463 ID=Msa1308850-mRNA-1:cds;Parent=Msa1308850-mRNA-1
chr8_4 CDS 5206849 5207650 5206849 ID=Msa1308850-mRNA-1:cds;Parent=Msa1308850-mRNA-1
chr8_4 three_prime_UTR 5207651 5207892 5207651 ID=Msa1308850-mRNA-1:three_prime_utr;Parent=Msa1308850-mRNA-1
Gene Sequence

>Msa1308850

ATGGGTTGCACAGCGTCTAAACTCGACAACGAAGAAACGGTACGGCGGTGCAAAGAACGCCGCCGTCTCATGAAGGAAGCAGTTTACGCTCGCCACTACCTCGCGGCGGCGCACTCTGATTACTGTAACTCTCTTCGCCTCACCGGTTCCGCACTCTCCACCTTCGCCGCCGGTGAACCTCTCGCTGTCTCCGACGACATTCCCGCCGTTTTCATCAACACAGTAAAAACTCGTCAGACACCGCCACCTCCTCCTCCAGCCACCGTAACAACCACAAAACTCCGTACAGTTTCTGAAACACGTCGCCGGAAAATTCCGGTGAAGCTTCCACACATTCTCTCCGATTCAAGCGTTTGTTCAATGCCGAGAAGCGAGTTTCCGAACTGGTTCATTCCAACCGCGCATCAAAATGAATCAACAGCTTCTGAAACTTCTTCCGTTTGGAACTGGGAGAATTTTCATCCTCCTCCTTCTCCTCCAAATTCCGAATATTTCCGGCAACGTTCTCAACCGCCGAATCATAAACCGGAAAATCCAGAATCCGATACATCATCAGAATCGTTCTACAACATGTTCCACTCGAAAATTCATCTTCATAATCAAAATCAGAATCAGAATCACAAGACTGAAACGGAACAAGAACTAGATCGATTGGAAACGGAACGTGAGGAGGTTCGGTGTAGTGATTGGGAGGATTGCTACAGTTCAACAAGCTCATCAGATAGAGATGAAGTTATCGCCGGTTTGAAGTCATCGGAAAAATCACCGGAATTTGTTGCCGGCGGTGAGGTTCCGGTGAGGAAAGACGAGGAGGTGAATGAAGAAAAGGTGAAGATGAAGCATAAGGATTTGAAAGAGATAGTTGAAGTGATAAGAGAGAATTTTGAGAAAGCGGCGGTGGCCGGAGATCGGTTATCAGAGATGTTGCAGATTAGTAGGGCTCAACTTGATCGGAGTTTCAGTCAATTGAAAAAAATAGTGTATCACTCTAGTAATATACTGAGCAAAGTGAGCTCAACTTGGACCTCAAAGCCACCATTGGCAGTTAAATACCGGTTTGATGCCGGTTCACTTGAAGGACCGGGCGGTCCAAAGAGCCTCTGCTCCACTTTGGACCGGCTTTTGGCATGGGAAAACAAACTCTTTCAGGAAGTGAAGGCTAGAGAAGGTGTGAAGATTGAGCATGAAAGGAAATTGTCAACTCTGCAGAGTGAAGAATGCAAGGGAGGTGATGACGAAACAAAGATAGACAAAACCAAGGCTACCATAACAAGGTTACAGTCACTAATTATTGTAACATCTCAGGCTGTGTCTACTACCTCAGCTGCCATCAATGGTCTTAGGGACTCTGATCTTGTTCCTCAGCTTGTTGAGCTTTGTCATGGAATCATTCACATGTGGAGATCAATGCACCAGTACCATGAAACGCAAAGCAACATTGTACAGCAAGTTTGTGGCCTTGTGAACCAATCATCAAAAGGTCACTCAACATCCGAATCACACAAACAGGCAACTCGAGATCTAGAATCAGCTCTGTCAGCTTGGCATTCAAGTTTTATCCGGATCATAAAATTTCAAAGAGACTTTATTTTGTCACTACATGGTTGGTTTAAGCTGAATCTTATTCCAGTCAACAATGACAACAACAACAGCAGCATTAGCAACAAAATGGAACCATCTGATGCATTTTCCTTTTGTGATGGATGGAAACTAGCCCTTGACCGTGTTCCAGACACTGTCGCTTCGGAAGCAATCAAAAGCTTCATCAATGTTGTCCACGTAATATCAGTTAAGCAATGTCAAGAACTCAAGATTAAAAGGAAAACTGACACTGCTTACAAGGAACTTGAGAAAAAATCTTCATCTCTAAAAAACATAGAAAAGAAGTTTTACAACTCATATTCTATGGTTGGTATTGGAATAATTCCAAACTCTGTGGAAGATATTGGACAAGGCTTGGATGCAAGAGACCCTCTTGCTGAGAAAAAATTGGAACTTGAAGCTTGTCAAAGACGTGTCGACGATGAAAAGTTGAAGCATTCGAAGGCTATTGAGGTTACAAGAGCTATGACACTTAATAATCTACAGACAGGTTTGCCTGGAATGTTTCAGGCATTGACAAGTTTTTCTTCATTGTTCATTGAAGCTCTTGAATCAGTATGTGACCGTTCATATGCCATAAAGTAG
Protein sequence

>Msa1308850

MGCTASKLDNEETVRRCKERRRLMKEAVYARHYLAAAHSDYCNSLRLTGSALSTFAAGEPLAVSDDIPAVFINTVKTRQTPPPPPPATVTTTKLRTVSETRRRKIPVKLPHILSDSSVCSMPRSEFPNWFIPTAHQNESTASETSSVWNWENFHPPPSPPNSEYFRQRSQPPNHKPENPESDTSSESFYNMFHSKIHLHNQNQNQNHKTETEQELDRLETEREEVRCSDWEDCYSSTSSSDRDEVIAGLKSSEKSPEFVAGGEVPVRKDEEVNEEKVKMKHKDLKEIVEVIRENFEKAAVAGDRLSEMLQISRAQLDRSFSQLKKIVYHSSNILSKVSSTWTSKPPLAVKYRFDAGSLEGPGGPKSLCSTLDRLLAWENKLFQEVKAREGVKIEHERKLSTLQSEECKGGDDETKIDKTKATITRLQSLIIVTSQAVSTTSAAINGLRDSDLVPQLVELCHGIIHMWRSMHQYHETQSNIVQQVCGLVNQSSKGHSTSESHKQATRDLESALSAWHSSFIRIIKFQRDFILSLHGWFKLNLIPVNNDNNNSSISNKMEPSDAFSFCDGWKLALDRVPDTVASEAIKSFINVVHVISVKQCQELKIKRKTDTAYKELEKKSSSLKNIEKKFYNSYSMVGIGIIPNSVEDIGQGLDARDPLAEKKLELEACQRRVDDEKLKHSKAIEVTRAMTLNNLQTGLPGMFQALTSFSSLFIEALESVCDRSYAIK*