Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016580 | Msa0731550 | 0.827656 | 1.386597e-54 | -8.615850e-47 |
| Msa0107580 | Msa0731550 | 0.834219 | 3.401376e-56 | -8.615850e-47 |
| Msa0110270 | Msa0731550 | 0.805750 | 1.147922e-49 | -8.615850e-47 |
| Msa0111510 | Msa0731550 | 0.816082 | 6.641685e-52 | -8.615850e-47 |
| Msa0120300 | Msa0731550 | 0.810524 | 1.104187e-50 | -8.615850e-47 |
| Msa0701010 | Msa0731550 | 0.816416 | 5.594068e-52 | -8.615850e-47 |
| Msa0731550 | Msa0731940 | 0.808142 | 3.581598e-50 | -8.615850e-47 |
| Msa0731550 | Msa0735170 | 0.802095 | 6.600669e-49 | -8.615850e-47 |
| Msa0731550 | Msa0766630 | 0.827420 | 1.579962e-54 | -8.615850e-47 |
| Msa0731550 | Msa0767720 | 0.803009 | 4.275809e-49 | -8.615850e-47 |
| Msa0731550 | Msa0768290 | 0.809918 | 1.491880e-50 | -8.615850e-47 |
| Msa0731550 | Msa0768370 | 0.840338 | 9.232257e-58 | -8.615850e-47 |
| Msa0731550 | Msa0789250 | 0.818472 | 1.926110e-52 | -8.615850e-47 |
| Msa0731550 | Msa0844490 | 0.804083 | 2.561314e-49 | -8.615850e-47 |
| Msa0731550 | Msa0863890 | 0.824283 | 8.780470e-54 | -8.615850e-47 |
| Msa0731550 | Msa0877250 | 0.841278 | 5.234138e-58 | -8.615850e-47 |
| Msa0731550 | Msa0885280 | 0.808802 | 2.589970e-50 | -8.615850e-47 |
| Msa0731550 | Msa0885290 | 0.802637 | 5.104228e-49 | -8.615850e-47 |
| Msa0731550 | Msa0899430 | 0.807109 | 5.935419e-50 | -8.615850e-47 |
| Msa0731550 | Msa0899450 | 0.810598 | 1.064698e-50 | -8.615850e-47 |
| Msa0731550 | Msa0907260 | 0.841947 | 3.488646e-58 | -8.615850e-47 |
| Msa0731550 | Msa0918700 | 0.832766 | 7.837865e-56 | -8.615850e-47 |
| Msa0731550 | Msa0927380 | 0.805673 | 1.191408e-49 | -8.615850e-47 |
| Msa0731550 | Msa0956270 | 0.839139 | 1.894159e-57 | -8.615850e-47 |
| Msa0731550 | Msa0958550 | 0.803139 | 4.020655e-49 | -8.615850e-47 |
| Msa0731550 | Msa0969520 | 0.805768 | 1.138250e-49 | -8.615850e-47 |
| Msa0731550 | Msa0976670 | 0.815642 | 8.327703e-52 | -8.615850e-47 |
| Msa0731550 | Msa0983090 | 0.808754 | 2.651609e-50 | -8.615850e-47 |
| Msa0731550 | Msa0991080 | 0.813170 | 2.931461e-51 | -8.615850e-47 |
| Msa0731550 | Msa1000900 | 0.802897 | 4.510012e-49 | -8.615850e-47 |
| Msa0731550 | Msa1014540 | 0.815531 | 8.813632e-52 | -8.615850e-47 |
| Msa0731550 | Msa1016280 | 0.808982 | 2.369629e-50 | -8.615850e-47 |
| Msa0731550 | Msa1085740 | 0.802148 | 6.437239e-49 | -8.615850e-47 |
| Msa0731550 | Msa1109440 | 0.801009 | 1.102245e-48 | -8.615850e-47 |
| Msa0731550 | Msa1111150 | 0.802788 | 4.750741e-49 | -8.615850e-47 |
| Msa0731550 | Msa1136090 | 0.811148 | 8.094054e-51 | -8.615850e-47 |
| Msa0731550 | Msa1156020 | 0.811136 | 8.142504e-51 | -8.615850e-47 |
| Msa0731550 | Msa1176760 | 0.800836 | 1.195484e-48 | -8.615850e-47 |
| Msa0731550 | Msa1230430 | 0.809399 | 1.929219e-50 | -8.615850e-47 |
| Msa0731550 | Msa1273920 | 0.814210 | 1.730019e-51 | -8.615850e-47 |
| Msa0731550 | Msa1276890 | 0.804317 | 2.289079e-49 | -8.615850e-47 |
| Msa0731550 | Msa1284680 | 0.836078 | 1.155406e-56 | -8.615850e-47 |
| Msa0731550 | Msa1342840 | 0.801673 | 8.059620e-49 | -8.615850e-47 |
| Msa0731550 | Msa1366810 | 0.827684 | 1.365334e-54 | -8.615850e-47 |
| Msa0731550 | Msa1402790 | 0.803953 | 2.724843e-49 | -8.615850e-47 |
| Msa0731550 | Msa1419030 | 0.800536 | 1.376224e-48 | -8.615850e-47 |
| Msa0131770 | Msa0731550 | 0.808307 | 3.302296e-50 | -8.615850e-47 |
| Msa0153910 | Msa0731550 | 0.821364 | 4.199779e-53 | -8.615850e-47 |
| Msa0198910 | Msa0731550 | 0.804013 | 2.648536e-49 | -8.615850e-47 |
| Msa0401310 | Msa0731550 | 0.825290 | 5.080202e-54 | -8.615850e-47 |
| Msa0402290 | Msa0731550 | 0.809131 | 2.202349e-50 | -8.615850e-47 |
| Msa0408110 | Msa0731550 | 0.842506 | 2.480437e-58 | -8.615850e-47 |
| Msa0424320 | Msa0731550 | 0.815260 | 1.012855e-51 | -8.615850e-47 |
| Msa0444130 | Msa0731550 | 0.801951 | 7.066122e-49 | -8.615850e-47 |
| Msa0450000 | Msa0731550 | 0.803657 | 3.139554e-49 | -8.615850e-47 |
| Msa0251320 | Msa0731550 | 0.810610 | 1.058139e-50 | -8.615850e-47 |
| Msa0251640 | Msa0731550 | 0.821245 | 4.474662e-53 | -8.615850e-47 |
| Msa0329190 | Msa0731550 | 0.813914 | 2.010886e-51 | -8.615850e-47 |
| Msa0355220 | Msa0731550 | 0.804574 | 2.023281e-49 | -8.615850e-47 |
| Msa0523940 | Msa0731550 | 0.801216 | 9.997512e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTACAGGTGGCTCTTT+GGG | 0.326536 | 5_1:-42553494 | Msa0731540:intron |
| GAATAGAGTGAATGGTTAGA+AGG | 0.349510 | 5_1:-42553536 | Msa0731540:intron |
| AATAGAGTGAATGGTTAGAA+GGG | 0.413335 | 5_1:-42553535 | Msa0731540:intron |
| AGGAAGTGAGGTGGGAATGT+AGG | 0.415816 | 5_1:-42553446 | Msa0731540:intron |
| GGAATTGAGGGGCTGTGTAT+CGG | 0.416756 | 5_1:-42553402 | Msa0731540:intron |
| TACAGGTGGCTCTTTGGGAT+TGG | 0.417710 | 5_1:-42553489 | Msa0731540:intron |
| GAATTGAGGGGCTGTGTATC+GGG | 0.444184 | 5_1:-42553401 | Msa0731540:intron |
| GTGAATGGTTAGAAGGGAGA+GGG | 0.447004 | 5_1:-42553529 | Msa0731540:intron |
| GGAAGTGAGGTGGGAATGTA+GGG | 0.449571 | 5_1:-42553445 | Msa0731540:intron |
| TGAATGGTTAGAAGGGAGAG+GGG | 0.492643 | 5_1:-42553528 | Msa0731540:intron |
| AGTGAATGGTTAGAAGGGAG+AGG | 0.492997 | 5_1:-42553530 | Msa0731540:intron |
| AGAGTATGAAAGTAGTTCAA+AGG | 0.501120 | 5_1:-42553466 | Msa0731540:intron |
| TGAGAGCAACCCCTCTGAGA+TGG | 0.503857 | 5_1:+42553372 | Msa0731550:CDS |
| TAGTTCAAAGGAAGTGAGGT+GGG | 0.514338 | 5_1:-42553454 | Msa0731540:intron |
| GAGTGTGAAGTCGGAATTGA+GGG | 0.525484 | 5_1:-42553414 | Msa0731540:intron |
| GAGAGCAACCCCTCTGAGAT+GGG | 0.536334 | 5_1:+42553373 | Msa0731550:CDS |
| CGGGTGATTCCCATCTCAGA+GGG | 0.543409 | 5_1:-42553382 | Msa0731540:intron |
| AAAGTAGTTCAAAGGAAGTG+AGG | 0.578660 | 5_1:-42553458 | Msa0731540:intron |
| GTAGTTCAAAGGAAGTGAGG+TGG | 0.600949 | 5_1:-42553455 | Msa0731540:intron |
| CAGAGGGGTTGCTCTCACTG+TGG | 0.624009 | 5_1:-42553366 | Msa0731540:intron |
| GTGTCTGATGAGTGTGAAGT+CGG | 0.647819 | 5_1:-42553423 | Msa0731540:intron |
| TGAGTGTGAAGTCGGAATTG+AGG | 0.651295 | 5_1:-42553415 | Msa0731540:intron |
| TCGGGTGATTCCCATCTCAG+AGG | 0.652573 | 5_1:-42553383 | Msa0731540:intron |
| GGGTGATTCCCATCTCAGAG+GGG | 0.753366 | 5_1:-42553381 | Msa0731540:intron |
| AGTGTGAAGTCGGAATTGAG+GGG | 0.779779 | 5_1:-42553413 | Msa0731540:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTATAACAAAAAGTAC+AGG | - | chr5_1:42553509-42553528 | Msa0731540:intron | 20.0% |
| !! | ATTATAACAAAAAGTACAGG+TGG | - | chr5_1:42553506-42553525 | Msa0731540:intron | 25.0% |
| ! | TTACTTTCTTTTTCTCGCAA+GGG | - | chr5_1:42553336-42553355 | Msa0731540:intron | 30.0% |
| ! | TTTACTTTCTTTTTCTCGCA+AGG | - | chr5_1:42553337-42553356 | Msa0731540:intron | 30.0% |
| AGAGTATGAAAGTAGTTCAA+AGG | - | chr5_1:42553469-42553488 | Msa0731540:intron | 30.0% | |
| ! | TACTTTCTTTTTCTCGCAAG+GGG | - | chr5_1:42553335-42553354 | Msa0731540:intron | 35.0% |
| AAAGTAGTTCAAAGGAAGTG+AGG | - | chr5_1:42553461-42553480 | Msa0731540:intron | 35.0% | |
| TAGTTCAAAGGAAGTGAGGT+GGG | - | chr5_1:42553457-42553476 | Msa0731540:intron | 40.0% | |
| ! | AAAAGTACAGGTGGCTCTTT+GGG | - | chr5_1:42553497-42553516 | Msa0731540:intron | 40.0% |
| ! | AAAAAGTACAGGTGGCTCTT+TGG | - | chr5_1:42553498-42553517 | Msa0731540:intron | 40.0% |
| AGTGTGAAGTCGGAATTGAG+GGG | - | chr5_1:42553416-42553435 | Msa0731540:intron | 45.0% | |
| GAGTGTGAAGTCGGAATTGA+GGG | - | chr5_1:42553417-42553436 | Msa0731540:intron | 45.0% | |
| TGAGTGTGAAGTCGGAATTG+AGG | - | chr5_1:42553418-42553437 | Msa0731540:intron | 45.0% | |
| GTGTCTGATGAGTGTGAAGT+CGG | - | chr5_1:42553426-42553445 | Msa0731540:intron | 45.0% | |
| GTAGTTCAAAGGAAGTGAGG+TGG | - | chr5_1:42553458-42553477 | Msa0731540:intron | 45.0% | |
| GAATTGAGGGGCTGTGTATC+GGG | - | chr5_1:42553404-42553423 | Msa0731540:intron | 50.0% | |
| GGAATTGAGGGGCTGTGTAT+CGG | - | chr5_1:42553405-42553424 | Msa0731540:intron | 50.0% | |
| GGAAGTGAGGTGGGAATGTA+GGG | - | chr5_1:42553448-42553467 | Msa0731540:intron | 50.0% | |
| AGGAAGTGAGGTGGGAATGT+AGG | - | chr5_1:42553449-42553468 | Msa0731540:intron | 50.0% | |
| !! | TACAGGTGGCTCTTTGGGAT+TGG | - | chr5_1:42553492-42553511 | Msa0731540:intron | 50.0% |
| ! | TGAGAGCAACCCCTCTGAGA+TGG | + | chr5_1:42553372-42553391 | Msa0731550:CDS | 55.0% |
| !! | GAGAGCAACCCCTCTGAGAT+GGG | + | chr5_1:42553373-42553392 | Msa0731550:CDS | 55.0% |
| GGGTGATTCCCATCTCAGAG+GGG | - | chr5_1:42553384-42553403 | Msa0731540:intron | 55.0% | |
| CGGGTGATTCCCATCTCAGA+GGG | - | chr5_1:42553385-42553404 | Msa0731540:intron | 55.0% | |
| TCGGGTGATTCCCATCTCAG+AGG | - | chr5_1:42553386-42553405 | Msa0731540:intron | 55.0% | |
| CAGAGGGGTTGCTCTCACTG+TGG | - | chr5_1:42553369-42553388 | Msa0731540:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 42553316 | 42553543 | 42553316 | ID=Msa0731550;Name=Msa0731550 |
| chr5_1 | mRNA | 42553316 | 42553543 | 42553316 | ID=Msa0731550-mRNA-1;Parent=Msa0731550;Name=Msa0731550-mRNA-1;_AED=0.26;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|75 |
| chr5_1 | exon | 42553316 | 42553543 | 42553316 | ID=Msa0731550-mRNA-1:exon:12265;Parent=Msa0731550-mRNA-1 |
| chr5_1 | CDS | 42553316 | 42553543 | 42553316 | ID=Msa0731550-mRNA-1:cds;Parent=Msa0731550-mRNA-1 |
| Gene Sequence |
| Protein sequence |
