AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1364350


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364350 MtrunA17_Chr1g0208721 88.835 206 17 2 1 203 1 203 3.26e-126 357
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364350 AT1G15140.2 63.536 181 59 3 16 191 11 189 3.36e-68 210
Msa1364350 AT1G15140.3 63.536 181 59 3 16 191 11 189 3.36e-68 210
Msa1364350 AT1G15140.1 65.517 174 53 3 16 184 11 182 6.41e-68 210

Find 83 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAACACCAAATCAAATTT+TGG 0.152902 tig0018115:-79412 None:intergenic
ATCGACCCACCCGAGAAATT+TGG 0.234673 tig0018115:+79297 Msa1364350:CDS
GGTCAGTTTCTGCCTCAATT+TGG 0.275334 tig0018115:+79355 Msa1364350:CDS
GTCAGTTTCTGCCTCAATTT+GGG 0.288727 tig0018115:+79356 Msa1364350:CDS
TGTTGAGCTTAGTCCGGTTA+TGG 0.301742 tig0018115:+79251 Msa1364350:CDS
TTGCCACTGGATCCGGAATT+AGG 0.330537 tig0018115:+79334 Msa1364350:CDS
GTGTGAGACGTGGCAAGGTC+AGG 0.331915 tig0018115:-79057 None:intergenic
CTTGTCTTTGCCACTGGATC+CGG 0.337767 tig0018115:+79327 Msa1364350:CDS
CAATTTGGGTTGAAAATGTT+AGG 0.341741 tig0018115:+79370 Msa1364350:CDS
GTTGAGCTTAGTCCGGTTAT+GGG 0.374160 tig0018115:+79252 Msa1364350:CDS
CTTAGTCCGGTTATGGGGAA+TGG 0.384177 tig0018115:+79258 Msa1364350:CDS
ACTGTTCTTGTCTTTGCCAC+TGG 0.391138 tig0018115:+79321 Msa1364350:CDS
GGCTAAAGTGCGCATGGGAG+TGG 0.410046 tig0018115:-78843 None:intergenic
CGTTGTGTGGGTTGAAGAAA+GGG 0.418149 tig0018115:+79223 Msa1364350:CDS
GCGTTGTGTGGGTTGAAGAA+AGG 0.428547 tig0018115:+79222 Msa1364350:CDS
CGTAGGATGGACATGGGAAA+GGG 0.429123 tig0018115:-78871 None:intergenic
GGCAGAAACTGACCTAATTC+CGG 0.434711 tig0018115:-79346 None:intergenic
TCGATCTCGGAGAGAGGAGC+TGG 0.438598 tig0018115:-78985 None:intergenic
TCAAACCCATTCCCCATAAC+CGG 0.441227 tig0018115:-79264 None:intergenic
TTGACGTACGGCGGCGGAAA+GGG 0.449809 tig0018115:-78944 None:intergenic
CTTGACGTACGGCGGCGGAA+AGG 0.459299 tig0018115:-78945 None:intergenic
TGACGGAGACGGTGGTTGGT+GGG 0.462815 tig0018115:-78910 None:intergenic
GCGTAGGATGGACATGGGAA+AGG 0.470876 tig0018115:-78872 None:intergenic
GTGACGGAGACGGTGGTTGG+TGG 0.471139 tig0018115:-78911 None:intergenic
ATAGCAAGAAACGACGGTTT+CGG 0.475246 tig0018115:-79120 None:intergenic
TACGTCAAGACACCACCGTC+TGG 0.479107 tig0018115:+78959 Msa1364350:CDS
GTAGGATGGACATGGGAAAG+GGG 0.482435 tig0018115:-78870 None:intergenic
TGTGAGACGTGGCAAGGTCA+GGG 0.483409 tig0018115:-79056 None:intergenic
TTAGTCCGGTTATGGGGAAT+GGG 0.485622 tig0018115:+79259 Msa1364350:CDS
GAGTGGAGGGTAAGAGGATG+AGG 0.493794 tig0018115:-78826 None:intergenic
CGTGGCAAGGTCAGGGGCGT+CGG 0.495943 tig0018115:-79049 None:intergenic
GGATGTTGTTGAGCTTAGTC+CGG 0.502498 tig0018115:+79245 Msa1364350:CDS
TAGGATGGACATGGGAAAGG+GGG 0.503705 tig0018115:-78869 None:intergenic
AAGAACAGTTCCAAATTTCT+CGG 0.503898 tig0018115:-79307 None:intergenic
GACGGAGACGGTGGTTGGTG+GGG 0.509831 tig0018115:-78909 None:intergenic
AGAACAGTTCCAAATTTCTC+GGG 0.523438 tig0018115:-79306 None:intergenic
GGGTGGGGCTAAAGTGCGCA+TGG 0.524410 tig0018115:-78849 None:intergenic
GGTTTAGATTGAAGCGGCGT+AGG 0.525093 tig0018115:-78888 None:intergenic
GCATGGGAGTGGAGGGTAAG+AGG 0.525884 tig0018115:-78832 None:intergenic
TAAAGTGCGCATGGGAGTGG+AGG 0.532726 tig0018115:-78840 None:intergenic
GACGGTGGTGTCTTGACGTA+CGG 0.543425 tig0018115:-78956 None:intergenic
GGTGTCTTGACGTACGGCGG+CGG 0.544339 tig0018115:-78950 None:intergenic
TCCACTGCAGAAGCGTTGTG+TGG 0.545176 tig0018115:+79210 Msa1364350:CDS
TAGATTGAAGCGGCGTAGGA+TGG 0.550133 tig0018115:-78884 None:intergenic
AAAGGGTTGCAAGTCGGTGA+CGG 0.550813 tig0018115:-78927 None:intergenic
GATGAGGCGGTGTTAATGAA+AGG 0.551705 tig0018115:-78810 None:intergenic
TTGCAAGTCGGTGACGGAGA+CGG 0.558431 tig0018115:-78921 None:intergenic
AGGGGCGTCGGAAACGTCGA+TGG 0.558624 tig0018115:-79037 None:intergenic
ACAGTTCCAAATTTCTCGGG+TGG 0.560614 tig0018115:-79303 None:intergenic
TCGGTGACGGAGACGGTGGT+TGG 0.560695 tig0018115:-78914 None:intergenic
GGAATGGGTTTGATATTAGT+CGG 0.561988 tig0018115:+79274 Msa1364350:CDS
CTCAGCGGCAGGTTCGATCT+CGG 0.562464 tig0018115:-78998 None:intergenic
CGCGTGTGTGAGACGTGGCA+AGG 0.563503 tig0018115:-79062 None:intergenic
CTCTTAATCGCAAGCTTCGG+CGG 0.564323 tig0018115:-79150 None:intergenic
TGGAAGAGTGACTCAGCGGC+AGG 0.570715 tig0018115:-79009 None:intergenic
GAGAGGAGCTGGAGTCCAGA+CGG 0.574346 tig0018115:-78974 None:intergenic
GGTGGGGCTAAAGTGCGCAT+GGG 0.577657 tig0018115:-78848 None:intergenic
TTGAGCTTAGTCCGGTTATG+GGG 0.580245 tig0018115:+79253 Msa1364350:CDS
CAGTTCCAAATTTCTCGGGT+GGG 0.584521 tig0018115:-79302 None:intergenic
CCACTCTTAATCGCAAGCTT+CGG 0.586640 tig0018115:-79153 None:intergenic
GTGAGACGTGGCAAGGTCAG+GGG 0.594184 tig0018115:-79055 None:intergenic
CCCACACAACGCTTCTGCAG+TGG 0.596333 tig0018115:-79211 None:intergenic
TGGTGGGGTTTAGATTGAAG+CGG 0.600108 tig0018115:-78894 None:intergenic
GAAGCGGCGTAGGATGGACA+TGG 0.600277 tig0018115:-78878 None:intergenic
GCAGGTTCGATCTCGGAGAG+AGG 0.610044 tig0018115:-78991 None:intergenic
CCGAAGCTTGCGATTAAGAG+TGG 0.610290 tig0018115:+79153 Msa1364350:CDS
CGGCGGAAAGGGTTGCAAGT+CGG 0.616607 tig0018115:-78933 None:intergenic
CCACTGCAGAAGCGTTGTGT+GGG 0.619285 tig0018115:+79211 Msa1364350:CDS
AGGAGCTGGAGTCCAGACGG+TGG 0.619490 tig0018115:-78971 None:intergenic
CAGTATCTTCAGCTTAAAGT+CGG 0.619863 tig0018115:+79090 Msa1364350:CDS
GCCACGTCTCACACACGCGC+CGG 0.620260 tig0018115:+79066 Msa1364350:CDS
AAAGTGCGCATGGGAGTGGA+GGG 0.625677 tig0018115:-78839 None:intergenic
TGACCTAATTCCGGATCCAG+TGG 0.627958 tig0018115:-79337 None:intergenic
TGGAGGGTAAGAGGATGAGG+CGG 0.630907 tig0018115:-78823 None:intergenic
TTTAAGCTGAAGATACTGAC+CGG 0.649623 tig0018115:-79085 None:intergenic
CGGAAACGTCGATGGAAACG+TGG 0.650313 tig0018115:-79029 None:intergenic
CAAGTCGGTGACGGAGACGG+TGG 0.668145 tig0018115:-78918 None:intergenic
GTTGTGTGGGTTGAAGAAAG+GGG 0.680851 tig0018115:+79224 Msa1364350:CDS
AAGCGGCGTAGGATGGACAT+GGG 0.692247 tig0018115:-78877 None:intergenic
ACCGGCGCGTGTGTGAGACG+TGG 0.704486 tig0018115:-79067 None:intergenic
GGTGGTGTCTTGACGTACGG+CGG 0.712276 tig0018115:-78953 None:intergenic
AACGTGGAAGAGTGACTCAG+CGG 0.719906 tig0018115:-79013 None:intergenic
GAAGCGATAGCAAGAAACGA+CGG 0.765356 tig0018115:-79126 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGAACAGTTCCAAATTTCT+CGG - tig0018115:79310-79329 None:intergenic 30.0%
! AACATTTTCAACCCAAATTG+AGG - tig0018115:79370-79389 None:intergenic 30.0%
!! CAATTTGGGTTGAAAATGTT+AGG + tig0018115:79370-79389 Msa1364350:CDS 30.0%
TTTAAGCTGAAGATACTGAC+CGG - tig0018115:79088-79107 None:intergenic 35.0%
CAGTATCTTCAGCTTAAAGT+CGG + tig0018115:79090-79109 Msa1364350:CDS 35.0%
!!! TTTTTGGTGAAGAGTGTTGT+TGG + tig0018115:79186-79205 Msa1364350:CDS 35.0%
!!! TTTTGGTGAAGAGTGTTGTT+GGG + tig0018115:79187-79206 Msa1364350:CDS 35.0%
! GGAATGGGTTTGATATTAGT+CGG + tig0018115:79274-79293 Msa1364350:CDS 35.0%
AGAACAGTTCCAAATTTCTC+GGG - tig0018115:79309-79328 None:intergenic 35.0%
ATAGCAAGAAACGACGGTTT+CGG - tig0018115:79123-79142 None:intergenic 40.0%
!! GAGTGGTGTGTTTGAGTTTT+TGG + tig0018115:79170-79189 Msa1364350:CDS 40.0%
GTCAGTTTCTGCCTCAATTT+GGG + tig0018115:79356-79375 Msa1364350:CDS 40.0%
! GATGAGGCGGTGTTAATGAA+AGG - tig0018115:78813-78832 None:intergenic 45.0%
TGGTGGGGTTTAGATTGAAG+CGG - tig0018115:78897-78916 None:intergenic 45.0%
GAAGCGATAGCAAGAAACGA+CGG - tig0018115:79129-79148 None:intergenic 45.0%
CCACTCTTAATCGCAAGCTT+CGG - tig0018115:79156-79175 None:intergenic 45.0%
! CGTTGTGTGGGTTGAAGAAA+GGG + tig0018115:79223-79242 Msa1364350:CDS 45.0%
! GTTGTGTGGGTTGAAGAAAG+GGG + tig0018115:79224-79243 Msa1364350:CDS 45.0%
GGATGTTGTTGAGCTTAGTC+CGG + tig0018115:79245-79264 Msa1364350:CDS 45.0%
TGTTGAGCTTAGTCCGGTTA+TGG + tig0018115:79251-79270 Msa1364350:CDS 45.0%
GTTGAGCTTAGTCCGGTTAT+GGG + tig0018115:79252-79271 Msa1364350:CDS 45.0%
TTGAGCTTAGTCCGGTTATG+GGG + tig0018115:79253-79272 Msa1364350:CDS 45.0%
TTAGTCCGGTTATGGGGAAT+GGG + tig0018115:79259-79278 Msa1364350:CDS 45.0%
TCAAACCCATTCCCCATAAC+CGG - tig0018115:79267-79286 None:intergenic 45.0%
CAGTTCCAAATTTCTCGGGT+GGG - tig0018115:79305-79324 None:intergenic 45.0%
ACAGTTCCAAATTTCTCGGG+TGG - tig0018115:79306-79325 None:intergenic 45.0%
ACTGTTCTTGTCTTTGCCAC+TGG + tig0018115:79321-79340 Msa1364350:CDS 45.0%
GGCAGAAACTGACCTAATTC+CGG - tig0018115:79349-79368 None:intergenic 45.0%
GGTCAGTTTCTGCCTCAATT+TGG + tig0018115:79355-79374 Msa1364350:CDS 45.0%
TAGGATGGACATGGGAAAGG+GGG - tig0018115:78872-78891 None:intergenic 50.0%
GTAGGATGGACATGGGAAAG+GGG - tig0018115:78873-78892 None:intergenic 50.0%
CGTAGGATGGACATGGGAAA+GGG - tig0018115:78874-78893 None:intergenic 50.0%
TAGATTGAAGCGGCGTAGGA+TGG - tig0018115:78887-78906 None:intergenic 50.0%
GGTTTAGATTGAAGCGGCGT+AGG - tig0018115:78891-78910 None:intergenic 50.0%
AAAGGGTTGCAAGTCGGTGA+CGG - tig0018115:78930-78949 None:intergenic 50.0%
AACGTGGAAGAGTGACTCAG+CGG - tig0018115:79016-79035 None:intergenic 50.0%
CTCTTAATCGCAAGCTTCGG+CGG - tig0018115:79153-79172 None:intergenic 50.0%
CCGAAGCTTGCGATTAAGAG+TGG + tig0018115:79153-79172 Msa1364350:CDS 50.0%
! GCGTTGTGTGGGTTGAAGAA+AGG + tig0018115:79222-79241 Msa1364350:CDS 50.0%
CTTAGTCCGGTTATGGGGAA+TGG + tig0018115:79258-79277 Msa1364350:CDS 50.0%
ATCGACCCACCCGAGAAATT+TGG + tig0018115:79297-79316 Msa1364350:CDS 50.0%
CTTGTCTTTGCCACTGGATC+CGG + tig0018115:79327-79346 Msa1364350:CDS 50.0%
TTGCCACTGGATCCGGAATT+AGG + tig0018115:79334-79353 Msa1364350:CDS 50.0%
TGACCTAATTCCGGATCCAG+TGG - tig0018115:79340-79359 None:intergenic 50.0%
TGGAGGGTAAGAGGATGAGG+CGG - tig0018115:78826-78845 None:intergenic 55.0%
GAGTGGAGGGTAAGAGGATG+AGG - tig0018115:78829-78848 None:intergenic 55.0%
AAAGTGCGCATGGGAGTGGA+GGG - tig0018115:78842-78861 None:intergenic 55.0%
TAAAGTGCGCATGGGAGTGG+AGG - tig0018115:78843-78862 None:intergenic 55.0%
ATGGACATGGGAAAGGGGGT+GGG - tig0018115:78868-78887 None:intergenic 55.0%
GCGTAGGATGGACATGGGAA+AGG - tig0018115:78875-78894 None:intergenic 55.0%
AAGCGGCGTAGGATGGACAT+GGG - tig0018115:78880-78899 None:intergenic 55.0%
!! TTGCAAGTCGGTGACGGAGA+CGG - tig0018115:78924-78943 None:intergenic 55.0%
GACGGTGGTGTCTTGACGTA+CGG - tig0018115:78959-78978 None:intergenic 55.0%
TACGTCAAGACACCACCGTC+TGG + tig0018115:78959-78978 Msa1364350:CDS 55.0%
CGGAAACGTCGATGGAAACG+TGG - tig0018115:79032-79051 None:intergenic 55.0%
TGTGAGACGTGGCAAGGTCA+GGG - tig0018115:79059-79078 None:intergenic 55.0%
!! TCCACTGCAGAAGCGTTGTG+TGG + tig0018115:79210-79229 Msa1364350:CDS 55.0%
!! CCACTGCAGAAGCGTTGTGT+GGG + tig0018115:79211-79230 Msa1364350:CDS 55.0%
GCATGGGAGTGGAGGGTAAG+AGG - tig0018115:78835-78854 None:intergenic 60.0%
GGCTAAAGTGCGCATGGGAG+TGG - tig0018115:78846-78865 None:intergenic 60.0%
GGTGGGGCTAAAGTGCGCAT+GGG - tig0018115:78851-78870 None:intergenic 60.0%
TGGACATGGGAAAGGGGGTG+GGG - tig0018115:78867-78886 None:intergenic 60.0%
GATGGACATGGGAAAGGGGG+TGG - tig0018115:78869-78888 None:intergenic 60.0%
GAAGCGGCGTAGGATGGACA+TGG - tig0018115:78881-78900 None:intergenic 60.0%
TGACGGAGACGGTGGTTGGT+GGG - tig0018115:78913-78932 None:intergenic 60.0%
CGGCGGAAAGGGTTGCAAGT+CGG - tig0018115:78936-78955 None:intergenic 60.0%
TTGACGTACGGCGGCGGAAA+GGG - tig0018115:78947-78966 None:intergenic 60.0%
GGTGGTGTCTTGACGTACGG+CGG - tig0018115:78956-78975 None:intergenic 60.0%
GAGAGGAGCTGGAGTCCAGA+CGG - tig0018115:78977-78996 None:intergenic 60.0%
TCGATCTCGGAGAGAGGAGC+TGG - tig0018115:78988-79007 None:intergenic 60.0%
GCAGGTTCGATCTCGGAGAG+AGG - tig0018115:78994-79013 None:intergenic 60.0%
CTCAGCGGCAGGTTCGATCT+CGG - tig0018115:79001-79020 None:intergenic 60.0%
TGGAAGAGTGACTCAGCGGC+AGG - tig0018115:79012-79031 None:intergenic 60.0%
GTGAGACGTGGCAAGGTCAG+GGG - tig0018115:79058-79077 None:intergenic 60.0%
GTGTGAGACGTGGCAAGGTC+AGG - tig0018115:79060-79079 None:intergenic 60.0%
CCCACACAACGCTTCTGCAG+TGG - tig0018115:79214-79233 None:intergenic 60.0%
GGGTGGGGCTAAAGTGCGCA+TGG - tig0018115:78852-78871 None:intergenic 65.0%
! GACGGAGACGGTGGTTGGTG+GGG - tig0018115:78912-78931 None:intergenic 65.0%
! GTGACGGAGACGGTGGTTGG+TGG - tig0018115:78914-78933 None:intergenic 65.0%
!! TCGGTGACGGAGACGGTGGT+TGG - tig0018115:78917-78936 None:intergenic 65.0%
!! CAAGTCGGTGACGGAGACGG+TGG - tig0018115:78921-78940 None:intergenic 65.0%
CTTGACGTACGGCGGCGGAA+AGG - tig0018115:78948-78967 None:intergenic 65.0%
GGTGTCTTGACGTACGGCGG+CGG - tig0018115:78953-78972 None:intergenic 65.0%
AGGAGCTGGAGTCCAGACGG+TGG - tig0018115:78974-78993 None:intergenic 65.0%
!! AGGGGCGTCGGAAACGTCGA+TGG - tig0018115:79040-79059 None:intergenic 65.0%
CGCGTGTGTGAGACGTGGCA+AGG - tig0018115:79065-79084 None:intergenic 65.0%
! CGTGGCAAGGTCAGGGGCGT+CGG - tig0018115:79052-79071 None:intergenic 70.0%
GCCACGTCTCACACACGCGC+CGG + tig0018115:79066-79085 Msa1364350:CDS 70.0%
ACCGGCGCGTGTGTGAGACG+TGG - tig0018115:79070-79089 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0018115 gene 78802 79422 78802 ID=Msa1364350;Name=Msa1364350
tig0018115 mRNA 78802 79422 78802 ID=Msa1364350-mRNA-1;Parent=Msa1364350;Name=Msa1364350-mRNA-1;_AED=0.02;_eAED=0.05;_QI=0|-1|0|1|-1|1|1|0|206
tig0018115 exon 78802 79422 78802 ID=Msa1364350-mRNA-1:exon:13915;Parent=Msa1364350-mRNA-1
tig0018115 CDS 78802 79422 78802 ID=Msa1364350-mRNA-1:cds;Parent=Msa1364350-mRNA-1
Gene Sequence

>Msa1364350

ATGTCACTCCTTTCATTAACACCGCCTCATCCTCTTACCCTCCACTCCCATGCGCACTTTAGCCCCACCCCCTTTCCCATGTCCATCCTACGCCGCTTCAATCTAAACCCCACCAACCACCGTCTCCGTCACCGACTTGCAACCCTTTCCGCCGCCGTACGTCAAGACACCACCGTCTGGACTCCAGCTCCTCTCTCCGAGATCGAACCTGCCGCTGAGTCACTCTTCCACGTTTCCATCGACGTTTCCGACGCCCCTGACCTTGCCACGTCTCACACACGCGCCGGTCAGTATCTTCAGCTTAAAGTCGGTGATTCGCCGAAACCGTCGTTTCTTGCTATCGCTTCGCCGCCGAAGCTTGCGATTAAGAGTGGTGTGTTTGAGTTTTTGGTGAAGAGTGTTGTTGGGTCCACTGCAGAAGCGTTGTGTGGGTTGAAGAAAGGGGATGTTGTTGAGCTTAGTCCGGTTATGGGGAATGGGTTTGATATTAGTCGGATCGACCCACCCGAGAAATTTGGAACTGTTCTTGTCTTTGCCACTGGATCCGGAATTAGGTCAGTTTCTGCCTCAATTTGGGTTGAAAATGTTAGGATTGTTATCTTATTATTTTCCAAAATTTGA

Protein sequence

>Msa1364350

MSLLSLTPPHPLTLHSHAHFSPTPFPMSILRRFNLNPTNHRLRHRLATLSAAVRQDTTVWTPAPLSEIEPAAESLFHVSIDVSDAPDLATSHTRAGQYLQLKVGDSPKPSFLAIASPPKLAIKSGVFEFLVKSVVGSTAEALCGLKKGDVVELSPVMGNGFDISRIDPPEKFGTVLVFATGSGIRSVSASIWVENVRIVILLFSKI*