AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1267190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1267190 MtrunA17_Chr4g0069811 90.173 173 6 5 6 170 1 170 7.46e-101 294
Msa1267190 MtrunA17_Chr2g0302031 47.297 148 50 8 39 170 8 143 6.65e-24 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1267190 AT4G33140.1 51.064 94 38 3 81 170 102 191 9.76e-18 79.7

Find 24 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGTGTACGAGTTCTTTA+AGG 0.194014 8_2:+72304630 Msa1267190:CDS
AATGATATTGGTGGGTTAAA+TGG 0.242698 8_2:+72303476 Msa1267190:CDS
GTTAAGGTTGATAATGATAT+TGG 0.344303 8_2:+72303464 Msa1267190:CDS
AACCCTATGGCTTATTCAAT+AGG 0.387771 8_2:+72303248 Msa1267190:CDS
GTTAATAGAAGATTTGAGTT+TGG 0.389742 8_2:+72303332 Msa1267190:CDS
GGAGCAAGGGAATTCTGTTA+AGG 0.434829 8_2:+72303448 Msa1267190:CDS
TGTTGCTGTTGATGTGGATG+AGG 0.476033 8_2:+72303550 Msa1267190:CDS
TTTGCATAATAACAACCCTA+TGG 0.504044 8_2:+72303235 Msa1267190:CDS
TAACCTATTGAATAAGCCAT+AGG 0.507567 8_2:-72303251 None:intergenic
GTTGCTGTTGATGTGGATGA+GGG 0.514964 8_2:+72303551 Msa1267190:CDS
TTTGCATCTGATTTGGAGCA+AGG 0.525020 8_2:+72303434 Msa1267190:CDS
TCCTTGTTCTCATTTGAGCA+AGG 0.527633 8_2:+72303283 Msa1267190:CDS
GCTTGTTGTTGCTGTTGATG+TGG 0.530835 8_2:+72303544 Msa1267190:CDS
AACCTATTGAATAAGCCATA+GGG 0.538609 8_2:-72303250 None:intergenic
TTGCATCTGATTTGGAGCAA+GGG 0.562036 8_2:+72303435 Msa1267190:CDS
AGGTTGATAATGATATTGGT+GGG 0.577068 8_2:+72303468 Msa1267190:CDS
AAGGTTGATAATGATATTGG+TGG 0.579097 8_2:+72303467 Msa1267190:CDS
CTAGTGCTAGTAATAGCAGT+AGG 0.623766 8_2:+72303378 Msa1267190:CDS
ACCTTGCTCAAATGAGAACA+AGG 0.632511 8_2:-72303284 None:intergenic
AGAATAGTGAAAATCTAGCA+CGG 0.647149 8_2:+72303195 Msa1267190:CDS
AAACGTTGATCCCGAAAACG+AGG 0.653630 8_2:-72303512 None:intergenic
AGTATTAGGAAATTTCGTGT+CGG 0.674898 8_2:+72304552 Msa1267190:CDS
AGAGCTAATGCAAGGCCTCG+CGG 0.678484 8_2:+72303410 Msa1267190:CDS
AAATCAGATGCAAAACCGCG+AGG 0.732365 8_2:-72303425 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTATAATAGCTTATCA+TGG + chr8_2:72303681-72303700 Msa1267190:intron 15.0%
!!! GATGTTTTAAAATAGAATAA+AGG + chr8_2:72304152-72304171 Msa1267190:intron 15.0%
!!! TATTTTATAATAGCTTATCA+TGG + chr8_2:72303681-72303700 Msa1267190:intron 15.0%
!!! GATGTTTTAAAATAGAATAA+AGG + chr8_2:72304152-72304171 Msa1267190:intron 15.0%
!!! TATGCTAATGTTTTTGATTT+TGG + chr8_2:72303308-72303327 Msa1267190:CDS 20.0%
!!! ATGCTAATGTTTTTGATTTT+GGG + chr8_2:72303309-72303328 Msa1267190:CDS 20.0%
!! GGAATCAATCTTTATATTAA+TGG + chr8_2:72303751-72303770 Msa1267190:intron 20.0%
!! ATCAATCTTTATATTAATGG+AGG + chr8_2:72303754-72303773 Msa1267190:intron 20.0%
!!! AAGAAAAAATAACTGATTCA+AGG - chr8_2:72304437-72304456 None:intergenic 20.0%
!!! TCAATTTTTTAAGCAGTATT+AGG + chr8_2:72304538-72304557 Msa1267190:intron 20.0%
!!! TATGCTAATGTTTTTGATTT+TGG + chr8_2:72303308-72303327 Msa1267190:CDS 20.0%
!!! ATGCTAATGTTTTTGATTTT+GGG + chr8_2:72303309-72303328 Msa1267190:CDS 20.0%
!! GGAATCAATCTTTATATTAA+TGG + chr8_2:72303751-72303770 Msa1267190:intron 20.0%
!! ATCAATCTTTATATTAATGG+AGG + chr8_2:72303754-72303773 Msa1267190:intron 20.0%
!!! AAGAAAAAATAACTGATTCA+AGG - chr8_2:72304437-72304456 None:intergenic 20.0%
!!! TCAATTTTTTAAGCAGTATT+AGG + chr8_2:72304538-72304557 Msa1267190:intron 20.0%
!!! TGCTAATGTTTTTGATTTTG+GGG + chr8_2:72303310-72303329 Msa1267190:CDS 25.0%
!! GTTAATAGAAGATTTGAGTT+TGG + chr8_2:72303332-72303351 Msa1267190:CDS 25.0%
!!! GTTAAGGTTGATAATGATAT+TGG + chr8_2:72303464-72303483 Msa1267190:CDS 25.0%
!!! TGTGTGTTTTTTAATATGCA+AGG + chr8_2:72303815-72303834 Msa1267190:intron 25.0%
! AGAACTTCACATTTAACAAA+AGG - chr8_2:72304062-72304081 None:intergenic 25.0%
!!! ACTGGATTATCTTTTTTGAA+AGG + chr8_2:72304115-72304134 Msa1267190:intron 25.0%
!!! TTCTTTAAGGTATCTTTTGT+TGG + chr8_2:72304643-72304662 Msa1267190:CDS 25.0%
!!! TGCTAATGTTTTTGATTTTG+GGG + chr8_2:72303310-72303329 Msa1267190:CDS 25.0%
!! GTTAATAGAAGATTTGAGTT+TGG + chr8_2:72303332-72303351 Msa1267190:CDS 25.0%
!!! GTTAAGGTTGATAATGATAT+TGG + chr8_2:72303464-72303483 Msa1267190:CDS 25.0%
!!! TGTGTGTTTTTTAATATGCA+AGG + chr8_2:72303815-72303834 Msa1267190:intron 25.0%
! AGAACTTCACATTTAACAAA+AGG - chr8_2:72304062-72304081 None:intergenic 25.0%
!!! ACTGGATTATCTTTTTTGAA+AGG + chr8_2:72304115-72304134 Msa1267190:intron 25.0%
!!! TTCTTTAAGGTATCTTTTGT+TGG + chr8_2:72304643-72304662 Msa1267190:CDS 25.0%
AGAATAGTGAAAATCTAGCA+CGG + chr8_2:72303195-72303214 Msa1267190:CDS 30.0%
TTTGCATAATAACAACCCTA+TGG + chr8_2:72303235-72303254 Msa1267190:CDS 30.0%
AACCTATTGAATAAGCCATA+GGG - chr8_2:72303253-72303272 None:intergenic 30.0%
TAACCTATTGAATAAGCCAT+AGG - chr8_2:72303254-72303273 None:intergenic 30.0%
! TTTTTGTAAGAGCTAATGCA+AGG + chr8_2:72303402-72303421 Msa1267190:CDS 30.0%
!! AAGGTTGATAATGATATTGG+TGG + chr8_2:72303467-72303486 Msa1267190:CDS 30.0%
!! AGGTTGATAATGATATTGGT+GGG + chr8_2:72303468-72303487 Msa1267190:CDS 30.0%
!! AATGATATTGGTGGGTTAAA+TGG + chr8_2:72303476-72303495 Msa1267190:CDS 30.0%
TAATATTGTTGCTCTCTGAT+AGG + chr8_2:72303727-72303746 Msa1267190:intron 30.0%
TGAACATATGCATGAAGATT+TGG - chr8_2:72303950-72303969 None:intergenic 30.0%
TACACATTACCAAGAATTTC+TGG - chr8_2:72304092-72304111 None:intergenic 30.0%
AGTATTAGGAAATTTCGTGT+CGG + chr8_2:72304552-72304571 Msa1267190:CDS 30.0%
AGAATAGTGAAAATCTAGCA+CGG + chr8_2:72303195-72303214 Msa1267190:CDS 30.0%
TTTGCATAATAACAACCCTA+TGG + chr8_2:72303235-72303254 Msa1267190:CDS 30.0%
AACCTATTGAATAAGCCATA+GGG - chr8_2:72303253-72303272 None:intergenic 30.0%
TAACCTATTGAATAAGCCAT+AGG - chr8_2:72303254-72303273 None:intergenic 30.0%
! TTTTTGTAAGAGCTAATGCA+AGG + chr8_2:72303402-72303421 Msa1267190:CDS 30.0%
!! AAGGTTGATAATGATATTGG+TGG + chr8_2:72303467-72303486 Msa1267190:CDS 30.0%
!! AGGTTGATAATGATATTGGT+GGG + chr8_2:72303468-72303487 Msa1267190:CDS 30.0%
!! AATGATATTGGTGGGTTAAA+TGG + chr8_2:72303476-72303495 Msa1267190:CDS 30.0%
TAATATTGTTGCTCTCTGAT+AGG + chr8_2:72303727-72303746 Msa1267190:intron 30.0%
TGAACATATGCATGAAGATT+TGG - chr8_2:72303950-72303969 None:intergenic 30.0%
TACACATTACCAAGAATTTC+TGG - chr8_2:72304092-72304111 None:intergenic 30.0%
AGTATTAGGAAATTTCGTGT+CGG + chr8_2:72304552-72304571 Msa1267190:CDS 30.0%
AACCCTATGGCTTATTCAAT+AGG + chr8_2:72303248-72303267 Msa1267190:CDS 35.0%
GAGTTTGGTAGAAAAACTTG+TGG + chr8_2:72303347-72303366 Msa1267190:CDS 35.0%
TGAATAATGAAGACCTTGTG+TGG + chr8_2:72303629-72303648 Msa1267190:intron 35.0%
ATATTTGCCGAAATCTTAGC+TGG + chr8_2:72303973-72303992 Msa1267190:intron 35.0%
ACATTGACCAGCTAAGATTT+CGG - chr8_2:72303983-72304002 None:intergenic 35.0%
! ATTCTTTTCAGCATGCAACT+AGG + chr8_2:72304015-72304034 Msa1267190:intron 35.0%
CTGAAGATACCAGAAATTCT+TGG + chr8_2:72304080-72304099 Msa1267190:intron 35.0%
!! TGTTTTGAGTGACTGATACA+TGG + chr8_2:72304184-72304203 Msa1267190:intron 35.0%
AATTGTGAATTGTCAACTCC+AGG + chr8_2:72304367-72304386 Msa1267190:intron 35.0%
CACACATCAGAGAATATATG+TGG - chr8_2:72304506-72304525 None:intergenic 35.0%
TCATGTGTACGAGTTCTTTA+AGG + chr8_2:72304630-72304649 Msa1267190:CDS 35.0%
AACCCTATGGCTTATTCAAT+AGG + chr8_2:72303248-72303267 Msa1267190:CDS 35.0%
GAGTTTGGTAGAAAAACTTG+TGG + chr8_2:72303347-72303366 Msa1267190:CDS 35.0%
TGAATAATGAAGACCTTGTG+TGG + chr8_2:72303629-72303648 Msa1267190:intron 35.0%
ATATTTGCCGAAATCTTAGC+TGG + chr8_2:72303973-72303992 Msa1267190:intron 35.0%
ACATTGACCAGCTAAGATTT+CGG - chr8_2:72303983-72304002 None:intergenic 35.0%
! ATTCTTTTCAGCATGCAACT+AGG + chr8_2:72304015-72304034 Msa1267190:intron 35.0%
CTGAAGATACCAGAAATTCT+TGG + chr8_2:72304080-72304099 Msa1267190:intron 35.0%
!! TGTTTTGAGTGACTGATACA+TGG + chr8_2:72304184-72304203 Msa1267190:intron 35.0%
AATTGTGAATTGTCAACTCC+AGG + chr8_2:72304367-72304386 Msa1267190:intron 35.0%
CACACATCAGAGAATATATG+TGG - chr8_2:72304506-72304525 None:intergenic 35.0%
TCATGTGTACGAGTTCTTTA+AGG + chr8_2:72304630-72304649 Msa1267190:CDS 35.0%
TCCTTGTTCTCATTTGAGCA+AGG + chr8_2:72303283-72303302 Msa1267190:CDS 40.0%
ACCTTGCTCAAATGAGAACA+AGG - chr8_2:72303287-72303306 None:intergenic 40.0%
CTAGTGCTAGTAATAGCAGT+AGG + chr8_2:72303378-72303397 Msa1267190:CDS 40.0%
!! TTTGCATCTGATTTGGAGCA+AGG + chr8_2:72303434-72303453 Msa1267190:CDS 40.0%
!! TTGCATCTGATTTGGAGCAA+GGG + chr8_2:72303435-72303454 Msa1267190:CDS 40.0%
TACAGTCAAGATTCCACACA+AGG - chr8_2:72303645-72303664 None:intergenic 40.0%
TATTGTTGCTCTCTGATAGG+AGG + chr8_2:72303730-72303749 Msa1267190:intron 40.0%
!! TCTTGGTAATGTGTAGTGAC+TGG + chr8_2:72304097-72304116 Msa1267190:intron 40.0%
TTGAAAGGATGTCTAGTGAC+TGG + chr8_2:72304130-72304149 Msa1267190:intron 40.0%
ATCAAAGATTCATGCACACC+TGG - chr8_2:72304388-72304407 None:intergenic 40.0%
TCCTTGTTCTCATTTGAGCA+AGG + chr8_2:72303283-72303302 Msa1267190:CDS 40.0%
ACCTTGCTCAAATGAGAACA+AGG - chr8_2:72303287-72303306 None:intergenic 40.0%
CTAGTGCTAGTAATAGCAGT+AGG + chr8_2:72303378-72303397 Msa1267190:CDS 40.0%
!! TTTGCATCTGATTTGGAGCA+AGG + chr8_2:72303434-72303453 Msa1267190:CDS 40.0%
!! TTGCATCTGATTTGGAGCAA+GGG + chr8_2:72303435-72303454 Msa1267190:CDS 40.0%
TACAGTCAAGATTCCACACA+AGG - chr8_2:72303645-72303664 None:intergenic 40.0%
TATTGTTGCTCTCTGATAGG+AGG + chr8_2:72303730-72303749 Msa1267190:intron 40.0%
!! TCTTGGTAATGTGTAGTGAC+TGG + chr8_2:72304097-72304116 Msa1267190:intron 40.0%
TTGAAAGGATGTCTAGTGAC+TGG + chr8_2:72304130-72304149 Msa1267190:intron 40.0%
ATCAAAGATTCATGCACACC+TGG - chr8_2:72304388-72304407 None:intergenic 40.0%
AAATCAGATGCAAAACCGCG+AGG - chr8_2:72303428-72303447 None:intergenic 45.0%
! TCGCGGTTTTGCATCTGATT+TGG + chr8_2:72303427-72303446 Msa1267190:CDS 45.0%
GGAGCAAGGGAATTCTGTTA+AGG + chr8_2:72303448-72303467 Msa1267190:CDS 45.0%
AAACGTTGATCCCGAAAACG+AGG - chr8_2:72303515-72303534 None:intergenic 45.0%
!! GCTTGTTGTTGCTGTTGATG+TGG + chr8_2:72303544-72303563 Msa1267190:CDS 45.0%
! TGTTGCTGTTGATGTGGATG+AGG + chr8_2:72303550-72303569 Msa1267190:CDS 45.0%
! GTTGCTGTTGATGTGGATGA+GGG + chr8_2:72303551-72303570 Msa1267190:CDS 45.0%
TCTTAGCTGGTCAATGTGTG+AGG + chr8_2:72303986-72304005 Msa1267190:intron 45.0%
CCAGCTATCATCAAACAGAG+GGG - chr8_2:72304223-72304242 None:intergenic 45.0%
GCCAGCTATCATCAAACAGA+GGG - chr8_2:72304224-72304243 None:intergenic 45.0%
AGCCAGCTATCATCAAACAG+AGG - chr8_2:72304225-72304244 None:intergenic 45.0%
TGAGACATCGCACTGAAAAC+AGG - chr8_2:72304414-72304433 None:intergenic 45.0%
AAATCAGATGCAAAACCGCG+AGG - chr8_2:72303428-72303447 None:intergenic 45.0%
! TCGCGGTTTTGCATCTGATT+TGG + chr8_2:72303427-72303446 Msa1267190:CDS 45.0%
GGAGCAAGGGAATTCTGTTA+AGG + chr8_2:72303448-72303467 Msa1267190:CDS 45.0%
AAACGTTGATCCCGAAAACG+AGG - chr8_2:72303515-72303534 None:intergenic 45.0%
!! GCTTGTTGTTGCTGTTGATG+TGG + chr8_2:72303544-72303563 Msa1267190:CDS 45.0%
! TGTTGCTGTTGATGTGGATG+AGG + chr8_2:72303550-72303569 Msa1267190:CDS 45.0%
! GTTGCTGTTGATGTGGATGA+GGG + chr8_2:72303551-72303570 Msa1267190:CDS 45.0%
TCTTAGCTGGTCAATGTGTG+AGG + chr8_2:72303986-72304005 Msa1267190:intron 45.0%
CCAGCTATCATCAAACAGAG+GGG - chr8_2:72304223-72304242 None:intergenic 45.0%
GCCAGCTATCATCAAACAGA+GGG - chr8_2:72304224-72304243 None:intergenic 45.0%
AGCCAGCTATCATCAAACAG+AGG - chr8_2:72304225-72304244 None:intergenic 45.0%
TGAGACATCGCACTGAAAAC+AGG - chr8_2:72304414-72304433 None:intergenic 45.0%
! GTTCTGCTACGCCTCGTTTT+CGG + chr8_2:72303501-72303520 Msa1267190:CDS 50.0%
! TTCTGCTACGCCTCGTTTTC+GGG + chr8_2:72303502-72303521 Msa1267190:CDS 50.0%
AGCTTATCATGGTGCTCGCA+TGG + chr8_2:72303692-72303711 Msa1267190:intron 50.0%
TAGCTGGTCAATGTGTGAGG+TGG + chr8_2:72303989-72304008 Msa1267190:intron 50.0%
! CCCCTCTGTTTGATGATAGC+TGG + chr8_2:72304220-72304239 Msa1267190:intron 50.0%
CTGGCTGCAATATTCTTGCC+TGG + chr8_2:72304239-72304258 Msa1267190:intron 50.0%
TTTCCAGCATGATCTGAGCC+AGG - chr8_2:72304260-72304279 None:intergenic 50.0%
! GTTCTGCTACGCCTCGTTTT+CGG + chr8_2:72303501-72303520 Msa1267190:CDS 50.0%
! TTCTGCTACGCCTCGTTTTC+GGG + chr8_2:72303502-72303521 Msa1267190:CDS 50.0%
AGCTTATCATGGTGCTCGCA+TGG + chr8_2:72303692-72303711 Msa1267190:intron 50.0%
TAGCTGGTCAATGTGTGAGG+TGG + chr8_2:72303989-72304008 Msa1267190:intron 50.0%
! CCCCTCTGTTTGATGATAGC+TGG + chr8_2:72304220-72304239 Msa1267190:intron 50.0%
CTGGCTGCAATATTCTTGCC+TGG + chr8_2:72304239-72304258 Msa1267190:intron 50.0%
TTTCCAGCATGATCTGAGCC+AGG - chr8_2:72304260-72304279 None:intergenic 50.0%
AGAGCTAATGCAAGGCCTCG+CGG + chr8_2:72303410-72303429 Msa1267190:CDS 55.0%
TTGCCTGGCTCAGATCATGC+TGG + chr8_2:72304254-72304273 Msa1267190:intron 55.0%
AGAGCTAATGCAAGGCCTCG+CGG + chr8_2:72303410-72303429 Msa1267190:CDS 55.0%
TTGCCTGGCTCAGATCATGC+TGG + chr8_2:72304254-72304273 Msa1267190:intron 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 72303167 72304693 72303167 ID=Msa1267190;Name=Msa1267190
chr8_2 mRNA 72303167 72304693 72303167 ID=Msa1267190-mRNA-1;Parent=Msa1267190;Name=Msa1267190-mRNA-1;_AED=0.05;_eAED=0.06;_QI=0|0|0|1|0|0.5|2|0|182
chr8_2 exon 72303167 72303572 72303167 ID=Msa1267190-mRNA-1:exon:24417;Parent=Msa1267190-mRNA-1
chr8_2 exon 72304551 72304693 72304551 ID=Msa1267190-mRNA-1:exon:24418;Parent=Msa1267190-mRNA-1
chr8_2 CDS 72303167 72303572 72303167 ID=Msa1267190-mRNA-1:cds;Parent=Msa1267190-mRNA-1
chr8_2 CDS 72304551 72304693 72304551 ID=Msa1267190-mRNA-1:cds;Parent=Msa1267190-mRNA-1
Gene Sequence

>Msa1267190

ATATTATATGTTATTATGTTTAGATTAAAGAATAGTGAAAATCTAGCACGGTTCTTGTATCACAAAACTTTGCATAATAACAACCCTATGGCTTATTCAATAGGTTATTATGTTGATCCTTGTTCTCATTTGAGCAAGGTTTATGCTAATGTTTTTGATTTTGGGGTTAATAGAAGATTTGAGTTTGGTAGAAAAACTTGTGGCTTTTATACTAGTGCTAGTAATAGCAGTAGGTTTTTTGTAAGAGCTAATGCAAGGCCTCGCGGTTTTGCATCTGATTTGGAGCAAGGGAATTCTGTTAAGGTTGATAATGATATTGGTGGGTTAAATGGAAGTTCTGCTACGCCTCGTTTTCGGGATCAACGTTTGTCTGAGAAGCTTGTTGTTGCTGTTGATGTGGATGAGGCAGTATTAGGAAATTTCGTGTCGGCTCTCAACAAGTACATTGCAGATCAATATTCATCAAATTATTCAGTTTCTGAATATCATGTGTACGAGTTCTTTAAGGTATCTTTTGTTGGCTGCTTTTGCAATTTCGTGTTTTCATGA

Protein sequence

>Msa1267190

ILYVIMFRLKNSENLARFLYHKTLHNNNPMAYSIGYYVDPCSHLSKVYANVFDFGVNRRFEFGRKTCGFYTSASNSSRFFVRANARPRGFASDLEQGNSVKVDNDIGGLNGSSATPRFRDQRLSEKLVVAVDVDEAVLGNFVSALNKYIADQYSSNYSVSEYHVYEFFKVSFVGCFCNFVFS*