Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1397260 | XP_003597774.1 | 98.071 | 311 | 1 | 2 | 1 | 306 | 1 | 311 | 0.0 | 620 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1397260 | sp|Q9S7T5|OFP14_ARATH | 36.025 | 322 | 145 | 10 | 1 | 295 | 1 | 288 | 5.75e-46 | 160 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1397260 | G7II51 | 98.071 | 311 | 1 | 2 | 1 | 306 | 1 | 311 | 0.0 | 620 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1397260 | TF | OFP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003560 | Msa1397260 | 0.804908 | 1.723721e-49 | -8.615850e-47 |
| Msa0029850 | Msa1397260 | 0.812381 | 4.363644e-51 | -8.615850e-47 |
| Msa0042550 | Msa1397260 | 0.819693 | 1.015601e-52 | -8.615850e-47 |
| Msa0048000 | Msa1397260 | 0.854670 | 1.054419e-61 | -8.615850e-47 |
| Msa0050760 | Msa1397260 | 0.800804 | 1.213830e-48 | -8.615850e-47 |
| Msa0085720 | Msa1397260 | 0.805791 | 1.125500e-49 | -8.615850e-47 |
| Msa0100240 | Msa1397260 | 0.803221 | 3.865690e-49 | -8.615850e-47 |
| Msa0108850 | Msa1397260 | 0.800162 | 1.640263e-48 | -8.615850e-47 |
| Msa1372150 | Msa1397260 | 0.821019 | 5.044619e-53 | -8.615850e-47 |
| Msa1373350 | Msa1397260 | 0.809653 | 1.701096e-50 | -8.615850e-47 |
| Msa1376890 | Msa1397260 | 0.831444 | 1.663751e-55 | -8.615850e-47 |
| Msa1379850 | Msa1397260 | 0.816291 | 5.964275e-52 | -8.615850e-47 |
| Msa1388450 | Msa1397260 | 0.807626 | 4.611870e-50 | -8.615850e-47 |
| Msa1397260 | Msa1401140 | 0.992567 | 4.516048e-194 | -8.615850e-47 |
| Msa1397260 | Msa1401530 | 0.814082 | 1.846885e-51 | -8.615850e-47 |
| Msa1397260 | Msa1404540 | 0.843869 | 1.074534e-58 | -8.615850e-47 |
| Msa1397260 | Msa1404670 | 0.813480 | 2.505557e-51 | -8.615850e-47 |
| Msa1397260 | Msa1408790 | 0.812465 | 4.182287e-51 | -8.615850e-47 |
| Msa1397260 | Msa1424680 | 0.813068 | 3.086540e-51 | -8.615850e-47 |
| Msa1397260 | Msa1431060 | 0.850109 | 2.099893e-60 | -8.615850e-47 |
| Msa1397260 | Msa1435400 | 0.847608 | 1.038187e-59 | -8.615850e-47 |
| Msa1397260 | Msa1436040 | 0.817793 | 2.743236e-52 | -8.615850e-47 |
| Msa1397260 | Msa1440600 | 0.801430 | 9.039620e-49 | -8.615850e-47 |
| Msa1397260 | Msa1440940 | 0.840055 | 1.094481e-57 | -8.615850e-47 |
| Msa1397260 | Msa1451620 | -0.804233 | 2.383889e-49 | -8.615850e-47 |
| Msa1397260 | Msa1455390 | 0.812091 | 5.047362e-51 | -8.615850e-47 |
| Msa1397260 | Msa1458550 | 0.805808 | 1.116237e-49 | -8.615850e-47 |
| Msa1397260 | Msa1463650 | -0.802758 | 4.819826e-49 | -8.615850e-47 |
| Msa0613460 | Msa1397260 | 0.826398 | 2.773587e-54 | -8.615850e-47 |
| Msa0626280 | Msa1397260 | 0.803528 | 3.339598e-49 | -8.615850e-47 |
| Msa0626990 | Msa1397260 | 0.807310 | 5.380063e-50 | -8.615850e-47 |
| Msa0627350 | Msa1397260 | 0.813933 | 1.991922e-51 | -8.615850e-47 |
| Msa0629630 | Msa1397260 | 0.810078 | 1.378099e-50 | -8.615850e-47 |
| Msa0651270 | Msa1397260 | 0.806441 | 8.212282e-50 | -8.615850e-47 |
| Msa0658570 | Msa1397260 | 0.823590 | 1.276346e-53 | -8.615850e-47 |
| Msa0677080 | Msa1397260 | 0.806535 | 7.845492e-50 | -8.615850e-47 |
| Msa0679550 | Msa1397260 | 0.804241 | 2.374642e-49 | -8.615850e-47 |
| Msa0693380 | Msa1397260 | 0.820581 | 6.360366e-53 | -8.615850e-47 |
| Msa0696410 | Msa1397260 | 0.821189 | 4.607491e-53 | -8.615850e-47 |
| Msa0703490 | Msa1397260 | 0.813019 | 3.164105e-51 | -8.615850e-47 |
| Msa0715110 | Msa1397260 | 0.807578 | 4.720564e-50 | -8.615850e-47 |
| Msa0724560 | Msa1397260 | 0.803027 | 4.241118e-49 | -8.615850e-47 |
| Msa0731470 | Msa1397260 | 0.824618 | 7.321825e-54 | -8.615850e-47 |
| Msa0735160 | Msa1397260 | 0.823017 | 1.736613e-53 | -8.615850e-47 |
| Msa0755070 | Msa1397260 | 0.806577 | 7.688951e-50 | -8.615850e-47 |
| Msa0757050 | Msa1397260 | 0.806855 | 6.714806e-50 | -8.615850e-47 |
| Msa0757080 | Msa1397260 | 0.832309 | 1.017227e-55 | -8.615850e-47 |
| Msa0766090 | Msa1397260 | 0.812484 | 4.141773e-51 | -8.615850e-47 |
| Msa0773310 | Msa1397260 | 0.846643 | 1.909826e-59 | -8.615850e-47 |
| Msa0798030 | Msa1397260 | 0.805876 | 1.080174e-49 | -8.615850e-47 |
| Msa0807030 | Msa1397260 | 0.810050 | 1.397908e-50 | -8.615850e-47 |
| Msa0809740 | Msa1397260 | 0.871220 | 8.067704e-67 | -8.615850e-47 |
| Msa0810760 | Msa1397260 | 0.834425 | 3.020525e-56 | -8.615850e-47 |
| Msa0818110 | Msa1397260 | 0.850952 | 1.217110e-60 | -8.615850e-47 |
| Msa0834440 | Msa1397260 | 0.808655 | 2.783301e-50 | -8.615850e-47 |
| Msa0124550 | Msa1397260 | 0.814581 | 1.432451e-51 | -8.615850e-47 |
| Msa0140910 | Msa1397260 | 0.807147 | 5.827093e-50 | -8.615850e-47 |
| Msa0144790 | Msa1397260 | 0.803350 | 3.636029e-49 | -8.615850e-47 |
| Msa0169820 | Msa1397260 | 0.814457 | 1.525903e-51 | -8.615850e-47 |
| Msa0178510 | Msa1397260 | 0.828906 | 6.927287e-55 | -8.615850e-47 |
| Msa0206790 | Msa1397260 | 0.812148 | 4.905961e-51 | -8.615850e-47 |
| Msa0219070 | Msa1397260 | 0.806944 | 6.430075e-50 | -8.615850e-47 |
| Msa0223380 | Msa1397260 | 0.807467 | 4.983898e-50 | -8.615850e-47 |
| Msa1247030 | Msa1397260 | 0.828048 | 1.116262e-54 | -8.615850e-47 |
| Msa1262990 | Msa1397260 | -0.802856 | 4.599325e-49 | -8.615850e-47 |
| Msa1264860 | Msa1397260 | -0.830109 | 3.532839e-55 | -8.615850e-47 |
| Msa1267060 | Msa1397260 | 0.820837 | 5.555048e-53 | -8.615850e-47 |
| Msa1267190 | Msa1397260 | 0.806022 | 1.006479e-49 | -8.615850e-47 |
| Msa1288340 | Msa1397260 | 0.805125 | 1.552121e-49 | -8.615850e-47 |
| Msa1299070 | Msa1397260 | 0.842918 | 1.928234e-58 | -8.615850e-47 |
| Msa1308590 | Msa1397260 | 0.801059 | 1.076663e-48 | -8.615850e-47 |
| Msa1335710 | Msa1397260 | 0.823561 | 1.296422e-53 | -8.615850e-47 |
| Msa0365490 | Msa1397260 | 0.806182 | 9.311726e-50 | -8.615850e-47 |
| Msa0377530 | Msa1397260 | 0.839361 | 1.659390e-57 | -8.615850e-47 |
| Msa0388030 | Msa1397260 | -0.853612 | 2.130400e-61 | -8.615850e-47 |
| Msa0392040 | Msa1397260 | -0.804275 | 2.336151e-49 | -8.615850e-47 |
| Msa0399300 | Msa1397260 | 0.803213 | 3.881188e-49 | -8.615850e-47 |
| Msa0422500 | Msa1397260 | 0.815284 | 1.000466e-51 | -8.615850e-47 |
| Msa0433410 | Msa1397260 | -0.800736 | 1.253256e-48 | -8.615850e-47 |
| Msa0436370 | Msa1397260 | 0.815508 | 8.919446e-52 | -8.615850e-47 |
| Msa0445280 | Msa1397260 | 0.813466 | 2.523446e-51 | -8.615850e-47 |
| Msa0450350 | Msa1397260 | 0.819248 | 1.282834e-52 | -8.615850e-47 |
| Msa0458440 | Msa1397260 | 0.813346 | 2.680987e-51 | -8.615850e-47 |
| Msa0462630 | Msa1397260 | 0.802472 | 5.521296e-49 | -8.615850e-47 |
| Msa0464900 | Msa1397260 | 0.854016 | 1.629637e-61 | -8.615850e-47 |
| Msa0469940 | Msa1397260 | -0.820478 | 6.716654e-53 | -8.615850e-47 |
| Msa0473240 | Msa1397260 | 0.829051 | 6.390245e-55 | -8.615850e-47 |
| Msa0480600 | Msa1397260 | 0.813145 | 2.968725e-51 | -8.615850e-47 |
| Msa1006850 | Msa1397260 | 0.834275 | 3.292661e-56 | -8.615850e-47 |
| Msa1021510 | Msa1397260 | 0.851852 | 6.774198e-61 | -8.615850e-47 |
| Msa1028270 | Msa1397260 | 0.814611 | 1.410552e-51 | -8.615850e-47 |
| Msa1041730 | Msa1397260 | 0.802049 | 6.744963e-49 | -8.615850e-47 |
| Msa1068200 | Msa1397260 | -0.819329 | 1.229727e-52 | -8.615850e-47 |
| Msa1073070 | Msa1397260 | -0.802391 | 5.737715e-49 | -8.615850e-47 |
| Msa1080160 | Msa1397260 | 0.841592 | 4.328080e-58 | -8.615850e-47 |
| Msa1082500 | Msa1397260 | 0.823634 | 1.246634e-53 | -8.615850e-47 |
| Msa1084260 | Msa1397260 | 0.811991 | 5.307318e-51 | -8.615850e-47 |
| Msa1087470 | Msa1397260 | 0.814832 | 1.260565e-51 | -8.615850e-47 |
| Msa1100480 | Msa1397260 | 0.825003 | 5.941245e-54 | -8.615850e-47 |
| Msa1105500 | Msa1397260 | 0.846672 | 1.874912e-59 | -8.615850e-47 |
| Msa0260810 | Msa1397260 | 0.848984 | 4.324359e-60 | -8.615850e-47 |
| Msa0266000 | Msa1397260 | -0.823865 | 1.100310e-53 | -8.615850e-47 |
| Msa0268090 | Msa1397260 | 0.819403 | 1.183087e-52 | -8.615850e-47 |
| Msa0288320 | Msa1397260 | 0.811899 | 5.557999e-51 | -8.615850e-47 |
| Msa0290650 | Msa1397260 | 0.805384 | 1.369917e-49 | -8.615850e-47 |
| Msa0292170 | Msa1397260 | 0.810971 | 8.841406e-51 | -8.615850e-47 |
| Msa0297960 | Msa1397260 | 0.820367 | 7.122734e-53 | -8.615850e-47 |
| Msa0326520 | Msa1397260 | 0.807123 | 5.895529e-50 | -8.615850e-47 |
| Msa0330390 | Msa1397260 | 0.804759 | 1.851697e-49 | -8.615850e-47 |
| Msa0335040 | Msa1397260 | 0.829222 | 5.809061e-55 | -8.615850e-47 |
| Msa0344690 | Msa1397260 | -0.800797 | 1.217792e-48 | -8.615850e-47 |
| Msa0344870 | Msa1397260 | 0.809524 | 1.813826e-50 | -8.615850e-47 |
| Msa0350840 | Msa1397260 | 0.808057 | 3.732961e-50 | -8.615850e-47 |
| Msa0356890 | Msa1397260 | -0.800239 | 1.582282e-48 | -8.615850e-47 |
| Msa1118760 | Msa1397260 | 0.817796 | 2.738750e-52 | -8.615850e-47 |
| Msa1124540 | Msa1397260 | -0.805717 | 1.166648e-49 | -8.615850e-47 |
| Msa1125080 | Msa1397260 | 0.803451 | 3.464341e-49 | -8.615850e-47 |
| Msa1127770 | Msa1397260 | 0.800610 | 1.329466e-48 | -8.615850e-47 |
| Msa1132970 | Msa1397260 | 0.814171 | 1.764829e-51 | -8.615850e-47 |
| Msa1135880 | Msa1397260 | 0.808429 | 3.111091e-50 | -8.615850e-47 |
| Msa1144720 | Msa1397260 | -0.813479 | 2.507155e-51 | -8.615850e-47 |
| Msa1152210 | Msa1397260 | 0.800645 | 1.307698e-48 | -8.615850e-47 |
| Msa1152950 | Msa1397260 | 0.816861 | 4.446538e-52 | -8.615850e-47 |
| Msa1156340 | Msa1397260 | -0.810044 | 1.401500e-50 | -8.615850e-47 |
| Msa1158660 | Msa1397260 | 0.807389 | 5.176041e-50 | -8.615850e-47 |
| Msa1162550 | Msa1397260 | -0.803901 | 2.793709e-49 | -8.615850e-47 |
| Msa1171730 | Msa1397260 | 0.805803 | 1.119131e-49 | -8.615850e-47 |
| Msa1192450 | Msa1397260 | 0.804683 | 1.920532e-49 | -8.615850e-47 |
| Msa1219810 | Msa1397260 | 0.811548 | 6.625667e-51 | -8.615850e-47 |
| Msa0484780 | Msa1397260 | 0.835609 | 1.519337e-56 | -8.615850e-47 |
| Msa0492880 | Msa1397260 | 0.805263 | 1.452570e-49 | -8.615850e-47 |
| Msa0511250 | Msa1397260 | 0.808190 | 3.498445e-50 | -8.615850e-47 |
| Msa0517490 | Msa1397260 | 0.850992 | 1.186125e-60 | -8.615850e-47 |
| Msa0530650 | Msa1397260 | 0.817594 | 3.040717e-52 | -8.615850e-47 |
| Msa0532540 | Msa1397260 | 0.800267 | 1.561411e-48 | -8.615850e-47 |
| Msa0558690 | Msa1397260 | 0.806233 | 9.088124e-50 | -8.615850e-47 |
| Msa0567020 | Msa1397260 | 0.831760 | 1.390566e-55 | -8.615850e-47 |
| Msa0572420 | Msa1397260 | 0.800253 | 1.571581e-48 | -8.615850e-47 |
| Msa0572480 | Msa1397260 | 0.818611 | 1.790697e-52 | -8.615850e-47 |
| Msa0580000 | Msa1397260 | 0.800901 | 1.159729e-48 | -8.615850e-47 |
| Msa0587190 | Msa1397260 | 0.808361 | 3.215970e-50 | -8.615850e-47 |
| Msa0598160 | Msa1397260 | 0.801545 | 8.558650e-49 | -8.615850e-47 |
| Msa0845070 | Msa1397260 | 0.803978 | 2.693670e-49 | -8.615850e-47 |
| Msa0850240 | Msa1397260 | 0.824022 | 1.011078e-53 | -8.615850e-47 |
| Msa0870530 | Msa1397260 | 0.815377 | 9.539940e-52 | -8.615850e-47 |
| Msa0903700 | Msa1397260 | 0.805477 | 1.309831e-49 | -8.615850e-47 |
| Msa0908170 | Msa1397260 | 0.837177 | 6.061186e-57 | -8.615850e-47 |
| Msa0936210 | Msa1397260 | 0.813513 | 2.464486e-51 | -8.615850e-47 |
| Msa0944730 | Msa1397260 | 0.813334 | 2.697812e-51 | -8.615850e-47 |
| Msa0973150 | Msa1397260 | 0.812389 | 4.346355e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1397260 | MtrunA17_Chr2g0331871 | 98.071 | 311 | 1 | 2 | 1 | 306 | 1 | 311 | 0.0 | 620 |
| Msa1397260 | MtrunA17_Chr4g0004341 | 47.950 | 317 | 127 | 9 | 1 | 306 | 1 | 290 | 3.60e-77 | 237 |
| Msa1397260 | MtrunA17_Chr1g0185481 | 38.462 | 143 | 63 | 5 | 133 | 268 | 142 | 266 | 4.32e-18 | 82.4 |
| Msa1397260 | MtrunA17_Chr7g0259991 | 47.887 | 71 | 35 | 1 | 198 | 268 | 132 | 200 | 3.74e-15 | 72.8 |
| Msa1397260 | MtrunA17_Chr1g0210351 | 28.689 | 244 | 96 | 9 | 29 | 265 | 26 | 198 | 1.54e-13 | 69.3 |
| Msa1397260 | MtrunA17_Chr1g0205341 | 42.188 | 64 | 34 | 1 | 203 | 266 | 117 | 177 | 3.70e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1397260 | AT1G79960.1 | 36.025 | 322 | 145 | 10 | 1 | 295 | 1 | 288 | 5.85e-47 | 160 |
| Msa1397260 | AT5G04820.1 | 35.338 | 133 | 62 | 4 | 134 | 266 | 102 | 210 | 2.82e-14 | 72.0 |
| Msa1397260 | AT4G14860.1 | 45.312 | 64 | 28 | 2 | 204 | 265 | 109 | 167 | 1.65e-11 | 62.4 |
Find 49 sgRNAs with CRISPR-Local
Find 128 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCAGGTTACCATAGAATT+TGG | 0.233067 | tig0022138:+87945 | Msa1397260:CDS |
| GAAAATTGATTGGGACTTCA+TGG | 0.309098 | tig0022138:+87749 | Msa1397260:CDS |
| TTTGCAGCCAGAGAGCATTT+TGG | 0.332403 | tig0022138:-87139 | None:intergenic |
| CAGGAACCACCAATACGTTT+TGG | 0.387081 | tig0022138:-87381 | None:intergenic |
| ACAATCAAAGGAAAATTGAT+TGG | 0.389239 | tig0022138:+87739 | Msa1397260:CDS |
| CTAAGAGGAACCAAATTCTA+TGG | 0.390296 | tig0022138:-87955 | None:intergenic |
| GTTAATCGAATTTGAGGCTG+TGG | 0.438345 | tig0022138:-87117 | None:intergenic |
| TATTGGTGGTTCCTGGTTGT+TGG | 0.439269 | tig0022138:+87389 | Msa1397260:CDS |
| TTAGGATTGGTAGGGAAGTC+AGG | 0.440786 | tig0022138:+87910 | Msa1397260:CDS |
| CAAAACGTATTGGTGGTTCC+TGG | 0.454127 | tig0022138:+87382 | Msa1397260:CDS |
| GAGTTGGTGGAATCAAAGTA+TGG | 0.465659 | tig0022138:+87705 | Msa1397260:CDS |
| TTGATCTCATCACTGTTATG+AGG | 0.467638 | tig0022138:+87841 | Msa1397260:CDS |
| GAAGAATTCAGAGGCTGCTC+CGG | 0.469764 | tig0022138:+87863 | Msa1397260:CDS |
| CGATTAACCAAAATGCTCTC+TGG | 0.470779 | tig0022138:+87132 | Msa1397260:CDS |
| CGTGTAGCGTGAGAACTGTT+AGG | 0.472831 | tig0022138:+87892 | Msa1397260:CDS |
| AACAGTGCAACACAATTCTC+AGG | 0.479484 | tig0022138:-87636 | None:intergenic |
| AGGTAACTCAGGATCATTGA+TGG | 0.483746 | tig0022138:+87479 | Msa1397260:CDS |
| ACACCACCTCAAGATCTTTG+TGG | 0.490451 | tig0022138:+87432 | Msa1397260:CDS |
| CAATCAAAGGAAAATTGATT+GGG | 0.491479 | tig0022138:+87740 | Msa1397260:CDS |
| TGAAATTCTCAAAGAGAAAA+CGG | 0.493044 | tig0022138:-87266 | None:intergenic |
| ATCAATGATCCTGAGTTACC+TGG | 0.499280 | tig0022138:-87477 | None:intergenic |
| AACAGCATCACCTTCAAAAG+AGG | 0.511692 | tig0022138:+87572 | Msa1397260:CDS |
| TGAGAACTGTTAGGATTGGT+AGG | 0.512241 | tig0022138:+87901 | Msa1397260:CDS |
| GCGATCAATGCAAGAGTTGG+TGG | 0.513161 | tig0022138:+87692 | Msa1397260:CDS |
| CTGAAATCGTCGTAAGGACT+CGG | 0.524949 | tig0022138:-87669 | None:intergenic |
| TTTGTTGAGGTAATCTTGAA+GGG | 0.525082 | tig0022138:-87079 | None:intergenic |
| CAGGCGATCAATGCAAGAGT+TGG | 0.529425 | tig0022138:+87689 | Msa1397260:CDS |
| AAATTCTATGGTAACCTGCT+TGG | 0.538786 | tig0022138:-87943 | None:intergenic |
| CTCACGCTACACGGCTTCGC+CGG | 0.538841 | tig0022138:-87882 | None:intergenic |
| TTTGTTGTTAACGGCGTTTG+AGG | 0.539359 | tig0022138:-87211 | None:intergenic |
| GAGGAACCACCAAAACGTAT+TGG | 0.549662 | tig0022138:+87372 | Msa1397260:CDS |
| GCACGATTCTTCGTGAAACC+AGG | 0.558059 | tig0022138:+87459 | Msa1397260:CDS |
| TATGTTTCTGCCGTTATCCA+AGG | 0.558329 | tig0022138:-87184 | None:intergenic |
| CTAACAGTTCTCACGCTACA+CGG | 0.576486 | tig0022138:-87891 | None:intergenic |
| TTAATCGAATTTGAGGCTGT+GGG | 0.576513 | tig0022138:-87116 | None:intergenic |
| TTCGTGAAACCAGGTAACTC+AGG | 0.588008 | tig0022138:+87468 | Msa1397260:CDS |
| AGCGTGAGAACTGTTAGGAT+TGG | 0.588361 | tig0022138:+87897 | Msa1397260:CDS |
| CAGGAAGAAGAAGACCAAGC+AGG | 0.606262 | tig0022138:+87929 | Msa1397260:CDS |
| TCTCGGTGACTCCAACAACC+AGG | 0.608261 | tig0022138:-87400 | None:intergenic |
| GTAAGATTGAAAACAATCAA+AGG | 0.608557 | tig0022138:+87727 | Msa1397260:CDS |
| GAACCACCAAAACGTATTGG+TGG | 0.614019 | tig0022138:+87375 | Msa1397260:CDS |
| GCTGAGCCACAAAGATCTTG+AGG | 0.618030 | tig0022138:-87438 | None:intergenic |
| TGTGATGATTGTGATCATCA+TGG | 0.625055 | tig0022138:-87608 | None:intergenic |
| GATCGCCTGAAATCGTCGTA+AGG | 0.629682 | tig0022138:-87675 | None:intergenic |
| GAGAACTGTTAGGATTGGTA+GGG | 0.631326 | tig0022138:+87902 | Msa1397260:CDS |
| GGTGGTGTTGTAATGAATCT+CGG | 0.636568 | tig0022138:-87417 | None:intergenic |
| AATTGATTGGGACTTCATGG+AGG | 0.677814 | tig0022138:+87752 | Msa1397260:CDS |
| TGTTATGAGGAAGAATTCAG+AGG | 0.682074 | tig0022138:+87854 | Msa1397260:CDS |
| GAGCCACAAAGATCTTGAGG+TGG | 0.753269 | tig0022138:-87435 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTGTTTTTGATTTTGTTG+AGG | - | tig0022138:87095-87114 | None:intergenic | 20.0% |
| !!! | ATCATTGATTTTGTTGTTAA+CGG | - | tig0022138:87223-87242 | None:intergenic | 20.0% |
| !!! | TTTTGTTTTTGATTTTGTTG+AGG | - | tig0022138:87095-87114 | None:intergenic | 20.0% |
| !!! | ATCATTGATTTTGTTGTTAA+CGG | - | tig0022138:87223-87242 | None:intergenic | 20.0% |
| !!! | ATTTTGGTTAATCGAATTTG+AGG | - | tig0022138:87126-87145 | None:intergenic | 25.0% |
| ! | TGAAATTCTCAAAGAGAAAA+CGG | - | tig0022138:87269-87288 | None:intergenic | 25.0% |
| ! | CAACAAAAACAAAAGAATCT+CGG | + | tig0022138:87335-87354 | Msa1397260:CDS | 25.0% |
| ! | GTAAGATTGAAAACAATCAA+AGG | + | tig0022138:87727-87746 | Msa1397260:CDS | 25.0% |
| ! | ACAATCAAAGGAAAATTGAT+TGG | + | tig0022138:87739-87758 | Msa1397260:CDS | 25.0% |
| ! | CAATCAAAGGAAAATTGATT+GGG | + | tig0022138:87740-87759 | Msa1397260:CDS | 25.0% |
| !!! | ATTTTGGTTAATCGAATTTG+AGG | - | tig0022138:87126-87145 | None:intergenic | 25.0% |
| ! | TGAAATTCTCAAAGAGAAAA+CGG | - | tig0022138:87269-87288 | None:intergenic | 25.0% |
| ! | CAACAAAAACAAAAGAATCT+CGG | + | tig0022138:87335-87354 | Msa1397260:CDS | 25.0% |
| ! | GTAAGATTGAAAACAATCAA+AGG | + | tig0022138:87727-87746 | Msa1397260:CDS | 25.0% |
| ! | ACAATCAAAGGAAAATTGAT+TGG | + | tig0022138:87739-87758 | Msa1397260:CDS | 25.0% |
| ! | CAATCAAAGGAAAATTGATT+GGG | + | tig0022138:87740-87759 | Msa1397260:CDS | 25.0% |
| TTTGTTGAGGTAATCTTGAA+GGG | - | tig0022138:87082-87101 | None:intergenic | 30.0% | |
| ! | TTTTGTTGAGGTAATCTTGA+AGG | - | tig0022138:87083-87102 | None:intergenic | 30.0% |
| ! | AAGGAAAAAGATGGTGTTTT+GGG | - | tig0022138:87168-87187 | None:intergenic | 30.0% |
| ! | ACTACAATAACCTCTTTTGA+AGG | - | tig0022138:87585-87604 | None:intergenic | 30.0% |
| TTTGTTGAGGTAATCTTGAA+GGG | - | tig0022138:87082-87101 | None:intergenic | 30.0% | |
| ! | TTTTGTTGAGGTAATCTTGA+AGG | - | tig0022138:87083-87102 | None:intergenic | 30.0% |
| ! | AAGGAAAAAGATGGTGTTTT+GGG | - | tig0022138:87168-87187 | None:intergenic | 30.0% |
| ! | ACTACAATAACCTCTTTTGA+AGG | - | tig0022138:87585-87604 | None:intergenic | 30.0% |
| !!! | AGGGTTTTTTGAAGCTTCTT+AGG | - | tig0022138:87063-87082 | None:intergenic | 35.0% |
| ! | TTAATCGAATTTGAGGCTGT+GGG | - | tig0022138:87119-87138 | None:intergenic | 35.0% |
| ! | CAAGGAAAAAGATGGTGTTT+TGG | - | tig0022138:87169-87188 | None:intergenic | 35.0% |
| ! | CAAAACACCATCTTTTTCCT+TGG | + | tig0022138:87167-87186 | Msa1397260:CDS | 35.0% |
| ! | CCATCTTTTTCCTTGGATAA+CGG | + | tig0022138:87174-87193 | Msa1397260:CDS | 35.0% |
| TGTGATGATTGTGATCATCA+TGG | - | tig0022138:87611-87630 | None:intergenic | 35.0% | |
| GAAAATTGATTGGGACTTCA+TGG | + | tig0022138:87749-87768 | Msa1397260:CDS | 35.0% | |
| TTGATCTCATCACTGTTATG+AGG | + | tig0022138:87841-87860 | Msa1397260:CDS | 35.0% | |
| TGTTATGAGGAAGAATTCAG+AGG | + | tig0022138:87854-87873 | Msa1397260:CDS | 35.0% | |
| AAATTCTATGGTAACCTGCT+TGG | - | tig0022138:87946-87965 | None:intergenic | 35.0% | |
| AAGCAGGTTACCATAGAATT+TGG | + | tig0022138:87945-87964 | Msa1397260:CDS | 35.0% | |
| CTAAGAGGAACCAAATTCTA+TGG | - | tig0022138:87958-87977 | None:intergenic | 35.0% | |
| !!! | AGGGTTTTTTGAAGCTTCTT+AGG | - | tig0022138:87063-87082 | None:intergenic | 35.0% |
| ! | TTAATCGAATTTGAGGCTGT+GGG | - | tig0022138:87119-87138 | None:intergenic | 35.0% |
| ! | CAAGGAAAAAGATGGTGTTT+TGG | - | tig0022138:87169-87188 | None:intergenic | 35.0% |
| ! | CAAAACACCATCTTTTTCCT+TGG | + | tig0022138:87167-87186 | Msa1397260:CDS | 35.0% |
| ! | CCATCTTTTTCCTTGGATAA+CGG | + | tig0022138:87174-87193 | Msa1397260:CDS | 35.0% |
| TGTGATGATTGTGATCATCA+TGG | - | tig0022138:87611-87630 | None:intergenic | 35.0% | |
| GAAAATTGATTGGGACTTCA+TGG | + | tig0022138:87749-87768 | Msa1397260:CDS | 35.0% | |
| TTGATCTCATCACTGTTATG+AGG | + | tig0022138:87841-87860 | Msa1397260:CDS | 35.0% | |
| TGTTATGAGGAAGAATTCAG+AGG | + | tig0022138:87854-87873 | Msa1397260:CDS | 35.0% | |
| AAATTCTATGGTAACCTGCT+TGG | - | tig0022138:87946-87965 | None:intergenic | 35.0% | |
| AAGCAGGTTACCATAGAATT+TGG | + | tig0022138:87945-87964 | Msa1397260:CDS | 35.0% | |
| CTAAGAGGAACCAAATTCTA+TGG | - | tig0022138:87958-87977 | None:intergenic | 35.0% | |
| ! | GTTAATCGAATTTGAGGCTG+TGG | - | tig0022138:87120-87139 | None:intergenic | 40.0% |
| CGATTAACCAAAATGCTCTC+TGG | + | tig0022138:87132-87151 | Msa1397260:CDS | 40.0% | |
| CCGTTATCCAAGGAAAAAGA+TGG | - | tig0022138:87177-87196 | None:intergenic | 40.0% | |
| ! | TATGTTTCTGCCGTTATCCA+AGG | - | tig0022138:87187-87206 | None:intergenic | 40.0% |
| !! | TTTGTTGTTAACGGCGTTTG+AGG | - | tig0022138:87214-87233 | None:intergenic | 40.0% |
| CTCGGATGAAAAAAACAACG+AGG | + | tig0022138:87353-87372 | Msa1397260:CDS | 40.0% | |
| !! | GGTGGTGTTGTAATGAATCT+CGG | - | tig0022138:87420-87439 | None:intergenic | 40.0% |
| ATCAATGATCCTGAGTTACC+TGG | - | tig0022138:87480-87499 | None:intergenic | 40.0% | |
| AGGTAACTCAGGATCATTGA+TGG | + | tig0022138:87479-87498 | Msa1397260:CDS | 40.0% | |
| AACAGCATCACCTTCAAAAG+AGG | + | tig0022138:87572-87591 | Msa1397260:CDS | 40.0% | |
| AACAGTGCAACACAATTCTC+AGG | - | tig0022138:87639-87658 | None:intergenic | 40.0% | |
| !! | GAGTTGGTGGAATCAAAGTA+TGG | + | tig0022138:87705-87724 | Msa1397260:CDS | 40.0% |
| AATTGATTGGGACTTCATGG+AGG | + | tig0022138:87752-87771 | Msa1397260:CDS | 40.0% | |
| ! | TGAGAACTGTTAGGATTGGT+AGG | + | tig0022138:87901-87920 | Msa1397260:CDS | 40.0% |
| ! | GAGAACTGTTAGGATTGGTA+GGG | + | tig0022138:87902-87921 | Msa1397260:CDS | 40.0% |
| ! | GTTAATCGAATTTGAGGCTG+TGG | - | tig0022138:87120-87139 | None:intergenic | 40.0% |
| CGATTAACCAAAATGCTCTC+TGG | + | tig0022138:87132-87151 | Msa1397260:CDS | 40.0% | |
| CCGTTATCCAAGGAAAAAGA+TGG | - | tig0022138:87177-87196 | None:intergenic | 40.0% | |
| ! | TATGTTTCTGCCGTTATCCA+AGG | - | tig0022138:87187-87206 | None:intergenic | 40.0% |
| !! | TTTGTTGTTAACGGCGTTTG+AGG | - | tig0022138:87214-87233 | None:intergenic | 40.0% |
| CTCGGATGAAAAAAACAACG+AGG | + | tig0022138:87353-87372 | Msa1397260:CDS | 40.0% | |
| !! | GGTGGTGTTGTAATGAATCT+CGG | - | tig0022138:87420-87439 | None:intergenic | 40.0% |
| ATCAATGATCCTGAGTTACC+TGG | - | tig0022138:87480-87499 | None:intergenic | 40.0% | |
| AGGTAACTCAGGATCATTGA+TGG | + | tig0022138:87479-87498 | Msa1397260:CDS | 40.0% | |
| AACAGCATCACCTTCAAAAG+AGG | + | tig0022138:87572-87591 | Msa1397260:CDS | 40.0% | |
| AACAGTGCAACACAATTCTC+AGG | - | tig0022138:87639-87658 | None:intergenic | 40.0% | |
| !! | GAGTTGGTGGAATCAAAGTA+TGG | + | tig0022138:87705-87724 | Msa1397260:CDS | 40.0% |
| AATTGATTGGGACTTCATGG+AGG | + | tig0022138:87752-87771 | Msa1397260:CDS | 40.0% | |
| ! | TGAGAACTGTTAGGATTGGT+AGG | + | tig0022138:87901-87920 | Msa1397260:CDS | 40.0% |
| ! | GAGAACTGTTAGGATTGGTA+GGG | + | tig0022138:87902-87921 | Msa1397260:CDS | 40.0% |
| !!! | TTTGCAGCCAGAGAGCATTT+TGG | - | tig0022138:87142-87161 | None:intergenic | 45.0% |
| GAGGAACCACCAAAACGTAT+TGG | + | tig0022138:87372-87391 | Msa1397260:CDS | 45.0% | |
| GAACCACCAAAACGTATTGG+TGG | + | tig0022138:87375-87394 | Msa1397260:CDS | 45.0% | |
| ! | GAACCACCAATACGTTTTGG+TGG | - | tig0022138:87381-87400 | None:intergenic | 45.0% |
| ! | CAGGAACCACCAATACGTTT+TGG | - | tig0022138:87384-87403 | None:intergenic | 45.0% |
| ! | CAAAACGTATTGGTGGTTCC+TGG | + | tig0022138:87382-87401 | Msa1397260:CDS | 45.0% |
| ! | TATTGGTGGTTCCTGGTTGT+TGG | + | tig0022138:87389-87408 | Msa1397260:CDS | 45.0% |
| ACACCACCTCAAGATCTTTG+TGG | + | tig0022138:87432-87451 | Msa1397260:CDS | 45.0% | |
| TTCGTGAAACCAGGTAACTC+AGG | + | tig0022138:87468-87487 | Msa1397260:CDS | 45.0% | |
| CTGAAATCGTCGTAAGGACT+CGG | - | tig0022138:87672-87691 | None:intergenic | 45.0% | |
| CTAACAGTTCTCACGCTACA+CGG | - | tig0022138:87894-87913 | None:intergenic | 45.0% | |
| AGCGTGAGAACTGTTAGGAT+TGG | + | tig0022138:87897-87916 | Msa1397260:CDS | 45.0% | |
| ! | TTAGGATTGGTAGGGAAGTC+AGG | + | tig0022138:87910-87929 | Msa1397260:CDS | 45.0% |
| !!! | TTTGCAGCCAGAGAGCATTT+TGG | - | tig0022138:87142-87161 | None:intergenic | 45.0% |
| GAGGAACCACCAAAACGTAT+TGG | + | tig0022138:87372-87391 | Msa1397260:CDS | 45.0% | |
| GAACCACCAAAACGTATTGG+TGG | + | tig0022138:87375-87394 | Msa1397260:CDS | 45.0% | |
| ! | GAACCACCAATACGTTTTGG+TGG | - | tig0022138:87381-87400 | None:intergenic | 45.0% |
| ! | CAGGAACCACCAATACGTTT+TGG | - | tig0022138:87384-87403 | None:intergenic | 45.0% |
| ! | CAAAACGTATTGGTGGTTCC+TGG | + | tig0022138:87382-87401 | Msa1397260:CDS | 45.0% |
| ! | TATTGGTGGTTCCTGGTTGT+TGG | + | tig0022138:87389-87408 | Msa1397260:CDS | 45.0% |
| ACACCACCTCAAGATCTTTG+TGG | + | tig0022138:87432-87451 | Msa1397260:CDS | 45.0% | |
| TTCGTGAAACCAGGTAACTC+AGG | + | tig0022138:87468-87487 | Msa1397260:CDS | 45.0% | |
| CTGAAATCGTCGTAAGGACT+CGG | - | tig0022138:87672-87691 | None:intergenic | 45.0% | |
| CTAACAGTTCTCACGCTACA+CGG | - | tig0022138:87894-87913 | None:intergenic | 45.0% | |
| AGCGTGAGAACTGTTAGGAT+TGG | + | tig0022138:87897-87916 | Msa1397260:CDS | 45.0% | |
| ! | TTAGGATTGGTAGGGAAGTC+AGG | + | tig0022138:87910-87929 | Msa1397260:CDS | 45.0% |
| GAGCCACAAAGATCTTGAGG+TGG | - | tig0022138:87438-87457 | None:intergenic | 50.0% | |
| GCTGAGCCACAAAGATCTTG+AGG | - | tig0022138:87441-87460 | None:intergenic | 50.0% | |
| GCACGATTCTTCGTGAAACC+AGG | + | tig0022138:87459-87478 | Msa1397260:CDS | 50.0% | |
| CGAGTCCTTACGACGATTTC+AGG | + | tig0022138:87670-87689 | Msa1397260:CDS | 50.0% | |
| GATCGCCTGAAATCGTCGTA+AGG | - | tig0022138:87678-87697 | None:intergenic | 50.0% | |
| CAGGCGATCAATGCAAGAGT+TGG | + | tig0022138:87689-87708 | Msa1397260:CDS | 50.0% | |
| GCGATCAATGCAAGAGTTGG+TGG | + | tig0022138:87692-87711 | Msa1397260:CDS | 50.0% | |
| GAAGAATTCAGAGGCTGCTC+CGG | + | tig0022138:87863-87882 | Msa1397260:CDS | 50.0% | |
| CGTGTAGCGTGAGAACTGTT+AGG | + | tig0022138:87892-87911 | Msa1397260:CDS | 50.0% | |
| CAGGAAGAAGAAGACCAAGC+AGG | + | tig0022138:87929-87948 | Msa1397260:CDS | 50.0% | |
| GAGCCACAAAGATCTTGAGG+TGG | - | tig0022138:87438-87457 | None:intergenic | 50.0% | |
| GCTGAGCCACAAAGATCTTG+AGG | - | tig0022138:87441-87460 | None:intergenic | 50.0% | |
| GCACGATTCTTCGTGAAACC+AGG | + | tig0022138:87459-87478 | Msa1397260:CDS | 50.0% | |
| CGAGTCCTTACGACGATTTC+AGG | + | tig0022138:87670-87689 | Msa1397260:CDS | 50.0% | |
| GATCGCCTGAAATCGTCGTA+AGG | - | tig0022138:87678-87697 | None:intergenic | 50.0% | |
| CAGGCGATCAATGCAAGAGT+TGG | + | tig0022138:87689-87708 | Msa1397260:CDS | 50.0% | |
| GCGATCAATGCAAGAGTTGG+TGG | + | tig0022138:87692-87711 | Msa1397260:CDS | 50.0% | |
| GAAGAATTCAGAGGCTGCTC+CGG | + | tig0022138:87863-87882 | Msa1397260:CDS | 50.0% | |
| CGTGTAGCGTGAGAACTGTT+AGG | + | tig0022138:87892-87911 | Msa1397260:CDS | 50.0% | |
| CAGGAAGAAGAAGACCAAGC+AGG | + | tig0022138:87929-87948 | Msa1397260:CDS | 50.0% | |
| TCTCGGTGACTCCAACAACC+AGG | - | tig0022138:87403-87422 | None:intergenic | 55.0% | |
| TCTCGGTGACTCCAACAACC+AGG | - | tig0022138:87403-87422 | None:intergenic | 55.0% | |
| CTCACGCTACACGGCTTCGC+CGG | - | tig0022138:87885-87904 | None:intergenic | 65.0% | |
| CTCACGCTACACGGCTTCGC+CGG | - | tig0022138:87885-87904 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0022138 | gene | 87057 | 87977 | 87057 | ID=Msa1397260;Name=Msa1397260 |
| tig0022138 | mRNA | 87057 | 87977 | 87057 | ID=Msa1397260-mRNA-1;Parent=Msa1397260;Name=Msa1397260-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|306 |
| tig0022138 | exon | 87057 | 87977 | 87057 | ID=Msa1397260-mRNA-1:exon:15901;Parent=Msa1397260-mRNA-1 |
| tig0022138 | CDS | 87057 | 87977 | 87057 | ID=Msa1397260-mRNA-1:cds;Parent=Msa1397260-mRNA-1 |
| Gene Sequence |
| Protein sequence |
