AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1439160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1439160 MtrunA17_Chr2g0306391 99.539 217 1 0 1 217 199 415 1.50e-161 452
Msa1439160 MtrunA17_Chr4g0062811 80.275 218 42 1 1 217 197 414 8.91e-128 367
Msa1439160 MtrunA17_Chr3g0132041 53.333 210 89 4 1 201 174 383 8.77e-71 220
Msa1439160 MtrunA17_Chr1g0208941 50.259 193 93 1 1 193 129 318 9.57e-66 207
Msa1439160 MtrunA17_Chr7g0235761 45.946 222 99 6 1 201 145 366 2.04e-56 183
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1439160 AT1G08510.1 79.703 202 40 1 1 201 201 402 2.39e-116 338
Msa1439160 AT3G25110.1 47.273 220 93 7 1 202 146 360 2.71e-60 193
Msa1439160 AT4G13050.1 43.498 223 101 5 1 201 145 364 2.01e-57 186

Find 131 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTAAATTGTTGTTATATA+TGG 0.196107 tig0028722:+40357 Msa1439160:three_prime_UTR
TCAAAATTGTTGATAGGTTT+TGG 0.207603 tig0028722:-40031 None:intergenic
CACTCACATCGCTGGCTTGC+TGG 0.220561 tig0028722:+36610 Msa1439160:five_prime_UTR
TGTCAAGATTTCCCCTGTTT+TGG 0.226691 tig0028722:-38811 None:intergenic
GAGTGCTCCACAGCCAATTT+TGG 0.252533 tig0028722:+39817 Msa1439160:intron
AACCGCAGTTCCGTTGATTT+TGG 0.259020 tig0028722:-37567 None:intergenic
GAAAACAAGTAAGGCTTTAT+AGG 0.268157 tig0028722:-40130 None:intergenic
TCCATCTTCCAATTCATATA+TGG 0.276238 tig0028722:+40271 Msa1439160:three_prime_UTR
AGCTCATGACTCTCCAAAAT+TGG 0.291578 tig0028722:-39830 None:intergenic
CGGCTTCTTGGTGATGGCTT+TGG 0.294450 tig0028722:+38096 Msa1439160:five_prime_UTR
ATAATGTTTGTTTGTGTGTT+AGG 0.297244 tig0028722:-36634 None:intergenic
ATGATGGTGCCGAGATTGTT+AGG 0.330723 tig0028722:+39990 Msa1439160:CDS
ATCTCTCCTCTGACCTCTTC+CGG 0.338263 tig0028722:-39206 None:intergenic
TGATAGTGAGTCAAACATTT+AGG 0.340023 tig0028722:-40083 None:intergenic
CTGTTATCCTCGTCCACAAC+TGG 0.341684 tig0028722:-39254 None:intergenic
TCATCAATCAGTTGCCTGAT+TGG 0.344132 tig0028722:+37648 Msa1439160:five_prime_UTR
CTTGTGCGGGATGCCAAAAC+AGG 0.344885 tig0028722:+38798 Msa1439160:CDS
GACGTTAATGAATCATTTGC+AGG 0.353941 tig0028722:+37945 Msa1439160:five_prime_UTR
TACCATAACCATATATGAAT+TGG 0.355189 tig0028722:-40279 None:intergenic
TTCCAATTCATATATGGTTA+TGG 0.356436 tig0028722:+40277 Msa1439160:three_prime_UTR
GAAGTACATTGGGTGGATTC+TGG 0.356450 tig0028722:+39464 Msa1439160:CDS
AGAACATCAAAATTGTTGAT+AGG 0.357493 tig0028722:-40037 None:intergenic
AAATCCAAGCAAAGCTCTAA+TGG 0.383578 tig0028722:+37515 Msa1439160:five_prime_UTR
AGCAGAACTAACTCCATGTT+TGG 0.390225 tig0028722:-37477 None:intergenic
GATGAAGATGATGATGTAAC+AGG 0.397644 tig0028722:-37434 None:intergenic
TATATGGTTATGGTACTGTT+AGG 0.400638 tig0028722:+40287 Msa1439160:three_prime_UTR
GACACTTGGGTTTCTGCGGC+AGG 0.408861 tig0028722:+38750 Msa1439160:CDS
ACTAACTAAACTTGATGATT+CGG 0.409649 tig0028722:+39280 Msa1439160:CDS
GACTGCCGGCTTCTTGGTGA+TGG 0.413628 tig0028722:+38090 Msa1439160:five_prime_UTR
AACATCTAGATCATTCCATC+TGG 0.417317 tig0028722:-39421 None:intergenic
GTTAATTCAACGGGTGAAAG+AGG 0.442886 tig0028722:+40187 Msa1439160:three_prime_UTR
CAGTCCCTGACATCTGTCTC+TGG 0.445222 tig0028722:+39905 Msa1439160:CDS
GCAAGAAGCATGCTCCAATC+AGG 0.445777 tig0028722:-37662 None:intergenic
TAATGTTTGTTTGTGTGTTA+GGG 0.448038 tig0028722:-36633 None:intergenic
CTAATATGTGTTAATTCAAC+GGG 0.450836 tig0028722:+40178 Msa1439160:three_prime_UTR
TGAGCTGCATTCCATGACTT+TGG 0.456877 tig0028722:+39847 Msa1439160:CDS
AAGAGAACTTTAAAAGCAGC+AGG 0.457388 tig0028722:+36520 None:intergenic
AAGGAAACTATCTAAAATTC+CGG 0.457461 tig0028722:+39187 Msa1439160:CDS
CAAGAGAATAGAGTTGAAAC+AGG 0.464573 tig0028722:-36772 None:intergenic
GTTAGTTCTGCTAACCTTGG+AGG 0.478639 tig0028722:+37488 Msa1439160:five_prime_UTR
GTGAGTGAGTGGCACAGAAT+TGG 0.479676 tig0028722:-36594 None:intergenic
TTCCTTCTGCTGAGAAGCAG+TGG 0.483344 tig0028722:+37700 Msa1439160:five_prime_UTR
AAAATGGTATGCGTCGTGAT+TGG 0.485644 tig0028722:+38775 Msa1439160:CDS
GAGATTGTTAGGGGTAGGAC+TGG 0.487479 tig0028722:+40001 Msa1439160:CDS
CTTAATCATGTTAAGACTGC+CGG 0.489292 tig0028722:+38076 Msa1439160:five_prime_UTR
TCTGTCTCTGGTGCTGACAT+TGG 0.489613 tig0028722:+39917 Msa1439160:CDS
AACTGATTGATGAATGTCCT+TGG 0.491484 tig0028722:-37638 None:intergenic
TGCTTCTCAGCAGAAGGAAA+AGG 0.493346 tig0028722:-37696 None:intergenic
TATCAGCTGGAGGTGCAGAT+TGG 0.494151 tig0028722:+40101 Msa1439160:three_prime_UTR
CAAAGCCATCACCAAGAAGC+CGG 0.494489 tig0028722:-38095 None:intergenic
TGATGGTGCCGAGATTGTTA+GGG 0.496988 tig0028722:+39991 Msa1439160:CDS
TTCTGATCCAGTTGTGGACG+AGG 0.498778 tig0028722:+39247 Msa1439160:CDS
ATATGGTTGTTACTCGATGC+AGG 0.498906 tig0028722:+38150 Msa1439160:exon
ACTATCTAAAATTCCGGAAG+AGG 0.500584 tig0028722:+39193 Msa1439160:CDS
TATATAACGAAGTGGCTGGC+TGG 0.505440 tig0028722:+36568 Msa1439160:five_prime_UTR
AACATTTAGGTGCTCTGTGA+TGG 0.506232 tig0028722:-40070 None:intergenic
ACCATATATGAATTGGAAGA+TGG 0.507891 tig0028722:-40272 None:intergenic
GTTAATAATGTGAAGTACAT+TGG 0.509594 tig0028722:+39453 Msa1439160:CDS
TCAACGGGTGAAAGAGGGAT+AGG 0.509607 tig0028722:+40193 Msa1439160:three_prime_UTR
GAGCAGAGGAAGAATAGAGT+AGG 0.510390 tig0028722:-40250 None:intergenic
ATGTTTGACTCACTATCAGC+TGG 0.510915 tig0028722:+40088 Msa1439160:three_prime_UTR
ATTCCATGACTTTGGAGTAC+AGG 0.511740 tig0028722:+39855 Msa1439160:CDS
CACTTGCTTCGACTCGATGA+TGG 0.516598 tig0028722:+39974 Msa1439160:CDS
AGAACTAACTCCATGTTTGG+AGG 0.519503 tig0028722:-37474 None:intergenic
TTGTATGTATAAATTGTTCG+TGG 0.520594 tig0028722:+37875 Msa1439160:five_prime_UTR
GTGCCACTCACTCACATCGC+TGG 0.520638 tig0028722:+36602 Msa1439160:five_prime_UTR
AGATCTCATGAAAACAAGTA+AGG 0.521133 tig0028722:-40139 None:intergenic
ACTAATATGTGTTAATTCAA+CGG 0.521513 tig0028722:+40177 Msa1439160:three_prime_UTR
TCGGCTGAATATATTCGCAC+TGG 0.521996 tig0028722:+39299 Msa1439160:CDS
TAAGCCATTAGAGCTTTGCT+TGG 0.523646 tig0028722:-37519 None:intergenic
TTAATAATGTGAAGTACATT+GGG 0.528885 tig0028722:+39454 Msa1439160:CDS
TTGTTACTCGATGCAGGTTG+TGG 0.529801 tig0028722:+38156 Msa1439160:exon
TATATATATAACGAAGTGGC+TGG 0.531033 tig0028722:+36564 Msa1439160:five_prime_UTR
TCATCTTCATCTGACAGTGT+TGG 0.533513 tig0028722:+37446 Msa1439160:five_prime_UTR
TGACTCTCCAAAATTGGCTG+TGG 0.535831 tig0028722:-39824 None:intergenic
CAGCACCAGAGACAGATGTC+AGG 0.537306 tig0028722:-39910 None:intergenic
GTCCTTGGGTGATGTTGTGA+TGG 0.537958 tig0028722:-37623 None:intergenic
AGATTGTTAGGGGTAGGACT+GGG 0.539935 tig0028722:+40002 Msa1439160:CDS
GGAGTTAGTTCTGCTAACCT+TGG 0.540076 tig0028722:+37485 Msa1439160:five_prime_UTR
GAGAACTTTAAAAGCAGCAG+GGG 0.541825 tig0028722:+36522 None:intergenic
TGAATTGGAAGATGGAGCAG+AGG 0.546647 tig0028722:-40264 None:intergenic
ATCCACTGCTTCTCAGCAGA+AGG 0.547180 tig0028722:-37702 None:intergenic
AAGTTGTTCAAGTAGACACT+TGG 0.551856 tig0028722:+38736 Msa1439160:CDS
GCGTCGTGATTGGCTTGTGC+GGG 0.553139 tig0028722:+38785 Msa1439160:CDS
TTGTTAGGGGTAGGACTGGG+TGG 0.554507 tig0028722:+40005 Msa1439160:CDS
TTGGAAATTTAGCTGACAGC+GGG 0.555268 tig0028722:+39936 Msa1439160:CDS
TGAAGAGAAGAGAAGAGAAG+AGG 0.555485 tig0028722:-36663 None:intergenic
TTGTGCGGGATGCCAAAACA+GGG 0.559601 tig0028722:+38799 Msa1439160:CDS
GCTTGGATTTGAATCCTCCA+AGG 0.561042 tig0028722:-37502 None:intergenic
AGGTGAAATCACAGCAGTCT+AGG 0.563931 tig0028722:-37736 None:intergenic
ACTGATTGATGAATGTCCTT+GGG 0.565331 tig0028722:-37637 None:intergenic
TGTTAATTCTGATCCAGTTG+TGG 0.567572 tig0028722:+39241 Msa1439160:CDS
TTAATTCAACGGGTGAAAGA+GGG 0.576794 tig0028722:+40188 Msa1439160:three_prime_UTR
AGTCCTACCCCTAACAATCT+CGG 0.577020 tig0028722:-39999 None:intergenic
AGTACAGGAGGGAATGTGGC+AGG 0.577889 tig0028722:+39870 Msa1439160:CDS
TGCGTCGTGATTGGCTTGTG+CGG 0.578969 tig0028722:+38784 Msa1439160:CDS
TTAGGGGTAGGACTGGGTGG+AGG 0.580415 tig0028722:+40008 Msa1439160:CDS
ATAATGTGAAGTACATTGGG+TGG 0.582097 tig0028722:+39457 Msa1439160:CDS
AAATCTTGACAAGAGCATCC+AGG 0.590317 tig0028722:+38823 Msa1439160:CDS
ACATCTAGATCATTCCATCT+GGG 0.591455 tig0028722:-39420 None:intergenic
CCATGACTTTGGAGTACAGG+AGG 0.592758 tig0028722:+39858 Msa1439160:CDS
CCTCCTGTACTCCAAAGTCA+TGG 0.597089 tig0028722:-39858 None:intergenic
GGTTACAACTACGACGGTGA+AGG 0.598952 tig0028722:+37586 Msa1439160:five_prime_UTR
CGTTATATATATAATCCACC+TGG 0.600787 tig0028722:-36554 None:intergenic
CAACGGGTGAAAGAGGGATA+GGG 0.600933 tig0028722:+40194 Msa1439160:three_prime_UTR
GTTGGAAAATCCTCCAAACA+TGG 0.605432 tig0028722:+37464 Msa1439160:five_prime_UTR
AGTTGTTCAAGTAGACACTT+GGG 0.611713 tig0028722:+38737 Msa1439160:CDS
TTGGAGTACAGGAGGGAATG+TGG 0.613380 tig0028722:+39866 Msa1439160:CDS
TTCTCAGCAGAAGGAAAAGG+TGG 0.618597 tig0028722:-37693 None:intergenic
AGAGAACTTTAAAAGCAGCA+GGG 0.621042 tig0028722:+36521 None:intergenic
AGCACCAGAGACAGATGTCA+GGG 0.622472 tig0028722:-39909 None:intergenic
CACCATCACAACATCACCCA+AGG 0.625250 tig0028722:+37621 Msa1439160:five_prime_UTR
AGTAGACACTTGGGTTTCTG+CGG 0.630478 tig0028722:+38746 Msa1439160:CDS
CATGACTTTGGAGTACAGGA+GGG 0.633124 tig0028722:+39859 Msa1439160:CDS
GATGGTGCCGAGATTGTTAG+GGG 0.638472 tig0028722:+39992 Msa1439160:CDS
GTGCCGAGATTGTTAGGGGT+AGG 0.642317 tig0028722:+39996 Msa1439160:CDS
AAGCCAGCGATGTGAGTGAG+TGG 0.643224 tig0028722:-36605 None:intergenic
TGGTTGATCGTTATCCGACG+TGG 0.644890 tig0028722:+38176 Msa1439160:CDS
GCAGCACCTTCCAAAATCAA+CGG 0.646470 tig0028722:+37557 Msa1439160:five_prime_UTR
TCATGATGAATAAACTGACA+AGG 0.652704 tig0028722:+39168 Msa1439160:CDS
AAAATTCCGGAAGAGGTCAG+AGG 0.654352 tig0028722:+39200 Msa1439160:CDS
GGATTATATATATAACGAAG+TGG 0.664627 tig0028722:+36560 Msa1439160:five_prime_UTR
ATTGGAAATTTAGCTGACAG+CGG 0.670615 tig0028722:+39935 Msa1439160:CDS
GTACATTGGGTGGATTCTGG+AGG 0.672791 tig0028722:+39467 Msa1439160:CDS
TTCCAAAATCAACGGAACTG+CGG 0.674773 tig0028722:+37565 Msa1439160:five_prime_UTR
TTTGACTCACTATCAGCTGG+AGG 0.685307 tig0028722:+40091 Msa1439160:three_prime_UTR
TCGATTGATTAGATCGAGTG+CGG 0.705741 tig0028722:+37904 Msa1439160:five_prime_UTR
AACTGCGGTTACAACTACGA+CGG 0.716495 tig0028722:+37580 Msa1439160:five_prime_UTR
AGAACTTTAAAAGCAGCAGG+GGG 0.739894 tig0028722:+36523 None:intergenic
TGTGCGGGATGCCAAAACAG+GGG 0.744085 tig0028722:+38800 Msa1439160:CDS
AAGGTTAAGACTAACCACGT+CGG 0.758321 tig0028722:-38190 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTGAACAAAAAAAAA+AGG - tig0028722:36977-36996 None:intergenic 10.0%
!!! TTTTTGTAATTTTTCTTTTT+GGG - tig0028722:37127-37146 None:intergenic 10.0%
!!! TTTTTTGTAATTTTTCTTTT+TGG - tig0028722:37128-37147 None:intergenic 10.0%
!!! ATTATCAATTTTTGTAAAAT+AGG - tig0028722:39005-39024 None:intergenic 10.0%
!!! ATTTAATTTTTTCTTCAAAA+TGG + tig0028722:40323-40342 Msa1439160:three_prime_UTR 10.0%
!!! TATTAAATTGTTGTTATATA+TGG + tig0028722:40357-40376 Msa1439160:three_prime_UTR 10.0%
!!! TATTGTTATTGTTTTTTTTC+TGG + tig0028722:37240-37259 Msa1439160:intron 15.0%
!!! TACAAACTTTTTATAAAAGT+AGG - tig0028722:38525-38544 None:intergenic 15.0%
!! ACTAAACAAATAATCAAAAA+GGG - tig0028722:39745-39764 None:intergenic 15.0%
!!! GAGATTTTGAATTTTGAATA+TGG - tig0028722:36705-36724 None:intergenic 20.0%
!!! AATTTTTTTCTTTCTGTTTG+TGG + tig0028722:36897-36916 Msa1439160:intron 20.0%
!! TTAAGTGATTATACAAAGTT+AGG - tig0028722:37167-37186 None:intergenic 20.0%
!! TGTATAATCACTTAAATTCA+AGG + tig0028722:37172-37191 Msa1439160:intron 20.0%
!!! TTGTTTTGTAGATGTTATTA+TGG + tig0028722:37323-37342 Msa1439160:intron 20.0%
!!! CTTATTATTTGTTTGATAAC+AGG + tig0028722:38045-38064 Msa1439160:intron 20.0%
!!! AGCTTTATACATATGAATTT+TGG + tig0028722:38432-38451 Msa1439160:intron 20.0%
!!! TATGCTTGAAATTATGTTTT+TGG + tig0028722:38657-38676 Msa1439160:intron 20.0%
!!! AACATTTTCAACATATCTTA+TGG - tig0028722:38891-38910 None:intergenic 20.0%
!! TTAATAATGTGAAGTACATT+GGG + tig0028722:39454-39473 Msa1439160:CDS 20.0%
!! ATCTTTGATAAACAAATCAA+TGG + tig0028722:39611-39630 Msa1439160:intron 20.0%
!! GACTAAACAAATAATCAAAA+AGG - tig0028722:39746-39765 None:intergenic 20.0%
!! TGTTTAGTCAATAAACTTAT+CGG + tig0028722:39757-39776 Msa1439160:intron 20.0%
!! ACTAATATGTGTTAATTCAA+CGG + tig0028722:40177-40196 Msa1439160:three_prime_UTR 20.0%
!! ACTTTCATTGGAAAAAAATA+AGG - tig0028722:40388-40407 None:intergenic 20.0%
! GGATTATATATATAACGAAG+TGG + tig0028722:36560-36579 Msa1439160:five_prime_UTR 25.0%
! TAATGTTTGTTTGTGTGTTA+GGG - tig0028722:36636-36655 None:intergenic 25.0%
! ATAATGTTTGTTTGTGTGTT+AGG - tig0028722:36637-36656 None:intergenic 25.0%
!! TCATTTTCTCTCATTCTTTA+AGG + tig0028722:36797-36816 Msa1439160:five_prime_UTR 25.0%
! ATAATCACTTAAATTCAAGG+TGG + tig0028722:37175-37194 Msa1439160:intron 25.0%
! ACAACAACAAAACAACAATT+TGG - tig0028722:37266-37285 None:intergenic 25.0%
! TTGTATGTATAAATTGTTCG+TGG + tig0028722:37875-37894 Msa1439160:five_prime_UTR 25.0%
!!! CTTATGTAACAAATTTGTTG+TGG + tig0028722:38000-38019 Msa1439160:intron 25.0%
! GCACATTTAACAAATCAAAA+AGG - tig0028722:38212-38231 None:intergenic 25.0%
! ATTTAACCTAATCTTCAATC+TGG - tig0028722:38379-38398 None:intergenic 25.0%
! AAGATTAGGTTAAATTCTTC+TGG + tig0028722:38384-38403 Msa1439160:intron 25.0%
! AGATTAGGTTAAATTCTTCT+GGG + tig0028722:38385-38404 Msa1439160:intron 25.0%
! ACAGGGTATGAAATATAAAA+TGG + tig0028722:38478-38497 Msa1439160:intron 25.0%
!! TTTTACCATAATATGTAGCT+AGG - tig0028722:38563-38582 None:intergenic 25.0%
!!! TTTTGCAATTTCTGATTTGT+TGG + tig0028722:39115-39134 Msa1439160:intron 25.0%
!! TTTTGATGTTTTACAGTGTT+TGG + tig0028722:39144-39163 Msa1439160:intron 25.0%
!! TTTGATGTTTTACAGTGTTT+GGG + tig0028722:39145-39164 Msa1439160:intron 25.0%
!! AAGGAAACTATCTAAAATTC+CGG + tig0028722:39187-39206 Msa1439160:CDS 25.0%
!! ACTAACTAAACTTGATGATT+CGG + tig0028722:39280-39299 Msa1439160:CDS 25.0%
! GTTAATAATGTGAAGTACAT+TGG + tig0028722:39453-39472 Msa1439160:CDS 25.0%
! ATATCAACAGTAGAATGAAT+TGG + tig0028722:39698-39717 Msa1439160:intron 25.0%
! GTTTAGTCAATAAACTTATC+GGG + tig0028722:39758-39777 Msa1439160:intron 25.0%
!!! TCAAAATTGTTGATAGGTTT+TGG - tig0028722:40034-40053 None:intergenic 25.0%
! AGAACATCAAAATTGTTGAT+AGG - tig0028722:40040-40059 None:intergenic 25.0%
! CTAATATGTGTTAATTCAAC+GGG + tig0028722:40178-40197 Msa1439160:three_prime_UTR 25.0%
! TTCCAATTCATATATGGTTA+TGG + tig0028722:40277-40296 Msa1439160:three_prime_UTR 25.0%
! TACCATAACCATATATGAAT+TGG - tig0028722:40282-40301 None:intergenic 25.0%
CGTTATATATATAATCCACC+TGG - tig0028722:36557-36576 None:intergenic 30.0%
TATATATATAACGAAGTGGC+TGG + tig0028722:36564-36583 Msa1439160:five_prime_UTR 30.0%
! CAAACATATTCCACACTTTT+TGG + tig0028722:36724-36743 Msa1439160:five_prime_UTR 30.0%
GAATTAGAATCCAAAAAGTG+TGG - tig0028722:36737-36756 None:intergenic 30.0%
! AAACAGGTCTGTATTGTTTT+TGG - tig0028722:36759-36778 None:intergenic 30.0%
!! TTTTGTTTCTTCTATGAAGC+TGG - tig0028722:36869-36888 None:intergenic 30.0%
!!! GTTGTTTTAAATTTGCATGC+AGG + tig0028722:37365-37384 Msa1439160:intron 30.0%
! AAGTTCTTTTTGCACATTTC+TGG - tig0028722:38129-38148 None:intergenic 30.0%
TGTGCAAAAAGAACTTGATA+TGG + tig0028722:38133-38152 Msa1439160:five_prime_UTR 30.0%
!! TGCTATCTCTATTTCTATCT+TGG + tig0028722:38249-38268 Msa1439160:intron 30.0%
! ATAGCTAAACTTTTTGTCTC+AGG + tig0028722:38341-38360 Msa1439160:intron 30.0%
TTTGGATTTCCTTTGTCTTT+AGG + tig0028722:38450-38469 Msa1439160:intron 30.0%
AACAATTTGCAAAGTACAGA+CGG - tig0028722:38622-38641 None:intergenic 30.0%
AGTGAATCTATATTTGCTTC+AGG + tig0028722:38709-38728 Msa1439160:intron 30.0%
GTGAATCTATATTTGCTTCA+GGG + tig0028722:38710-38729 Msa1439160:intron 30.0%
TGAATCTATATTTGCTTCAG+GGG + tig0028722:38711-38730 Msa1439160:intron 30.0%
GCTTGAAAGATTAAAAACAG+TGG - tig0028722:38947-38966 None:intergenic 30.0%
!! TCAGTTTGCTTTTGTCAAAT+CGG + tig0028722:39032-39051 Msa1439160:intron 30.0%
TCATGATGAATAAACTGACA+AGG + tig0028722:39168-39187 Msa1439160:CDS 30.0%
TAGTAGAGATTGTCTGAATA+AGG + tig0028722:39575-39594 Msa1439160:intron 30.0%
! TGATAGTGAGTCAAACATTT+AGG - tig0028722:40086-40105 None:intergenic 30.0%
! GAAAACAAGTAAGGCTTTAT+AGG - tig0028722:40133-40152 None:intergenic 30.0%
AGATCTCATGAAAACAAGTA+AGG - tig0028722:40142-40161 None:intergenic 30.0%
TCCATCTTCCAATTCATATA+TGG + tig0028722:40271-40290 Msa1439160:three_prime_UTR 30.0%
ACCATATATGAATTGGAAGA+TGG - tig0028722:40275-40294 None:intergenic 30.0%
TATATGGTTATGGTACTGTT+AGG + tig0028722:40287-40306 Msa1439160:three_prime_UTR 30.0%
CAAGAGAATAGAGTTGAAAC+AGG - tig0028722:36775-36794 None:intergenic 35.0%
! AGGAATAAAACGCATCGTTT+TGG - tig0028722:36825-36844 None:intergenic 35.0%
! CTTTCTGTTTGTGGCTTTTA+AGG + tig0028722:36906-36925 Msa1439160:intron 35.0%
TGCAGGTGTAAAAATCAACA+TGG + tig0028722:37382-37401 Msa1439160:intron 35.0%
! GATGAAGATGATGATGTAAC+AGG - tig0028722:37437-37456 None:intergenic 35.0%
! AAATCCAAGCAAAGCTCTAA+TGG + tig0028722:37515-37534 Msa1439160:five_prime_UTR 35.0%
ACTGATTGATGAATGTCCTT+GGG - tig0028722:37640-37659 None:intergenic 35.0%
AACTGATTGATGAATGTCCT+TGG - tig0028722:37641-37660 None:intergenic 35.0%
AAGAGAACAAGCAAGAGAAA+GGG - tig0028722:37774-37793 None:intergenic 35.0%
AAAGAGAACAAGCAAGAGAA+AGG - tig0028722:37775-37794 None:intergenic 35.0%
TAGGAACAAGCAAAGGAAAA+AGG - tig0028722:37815-37834 None:intergenic 35.0%
AAAGCAATAGGAACAAGCAA+AGG - tig0028722:37822-37841 None:intergenic 35.0%
GACGAAAAAAGAACAACGAA+AGG - tig0028722:37849-37868 None:intergenic 35.0%
GACGTTAATGAATCATTTGC+AGG + tig0028722:37945-37964 Msa1439160:five_prime_UTR 35.0%
CTTAATCATGTTAAGACTGC+CGG + tig0028722:38076-38095 Msa1439160:five_prime_UTR 35.0%
ATAGCACCAGATTGAAGATT+AGG + tig0028722:38370-38389 Msa1439160:intron 35.0%
TTTGTCTTTAGGCTGTTACA+GGG + tig0028722:38461-38480 Msa1439160:intron 35.0%
! GTTAGCCTAGCTACATATTA+TGG + tig0028722:38555-38574 Msa1439160:intron 35.0%
AAGTTGTTCAAGTAGACACT+TGG + tig0028722:38736-38755 Msa1439160:CDS 35.0%
AGTTGTTCAAGTAGACACTT+GGG + tig0028722:38737-38756 Msa1439160:CDS 35.0%
!! GTTCTGAATTGACTTTTACC+TGG - tig0028722:38844-38863 None:intergenic 35.0%
! ACTATCTAAAATTCCGGAAG+AGG + tig0028722:39193-39212 Msa1439160:CDS 35.0%
TGTTAATTCTGATCCAGTTG+TGG + tig0028722:39241-39260 Msa1439160:CDS 35.0%
ACATCTAGATCATTCCATCT+GGG - tig0028722:39423-39442 None:intergenic 35.0%
AACATCTAGATCATTCCATC+TGG - tig0028722:39424-39443 None:intergenic 35.0%
ATAATGTGAAGTACATTGGG+TGG + tig0028722:39457-39476 Msa1439160:CDS 35.0%
! GAATGAATTGGTCACTTTAG+AGG + tig0028722:39710-39729 Msa1439160:intron 35.0%
ATTGGAAATTTAGCTGACAG+CGG + tig0028722:39935-39954 Msa1439160:CDS 35.0%
TTAATTCAACGGGTGAAAGA+GGG + tig0028722:40188-40207 Msa1439160:three_prime_UTR 35.0%
TGAAGAGAAGAGAAGAGAAG+AGG - tig0028722:36666-36685 None:intergenic 40.0%
TGTTTCTTCTATGAAGCTGG+TGG - tig0028722:36866-36885 None:intergenic 40.0%
GAAAGTCTCAAATGAAGCTC+AGG - tig0028722:37027-37046 None:intergenic 40.0%
TCATCTTCATCTGACAGTGT+TGG + tig0028722:37446-37465 Msa1439160:five_prime_UTR 40.0%
GTTGGAAAATCCTCCAAACA+TGG + tig0028722:37464-37483 Msa1439160:five_prime_UTR 40.0%
AGAACTAACTCCATGTTTGG+AGG - tig0028722:37477-37496 None:intergenic 40.0%
AGCAGAACTAACTCCATGTT+TGG - tig0028722:37480-37499 None:intergenic 40.0%
TAAGCCATTAGAGCTTTGCT+TGG - tig0028722:37522-37541 None:intergenic 40.0%
TTCCAAAATCAACGGAACTG+CGG + tig0028722:37565-37584 Msa1439160:five_prime_UTR 40.0%
TCATCAATCAGTTGCCTGAT+TGG + tig0028722:37648-37667 Msa1439160:five_prime_UTR 40.0%
AAGCAAGAGAAAGGGAAAAC+AGG - tig0028722:37766-37785 None:intergenic 40.0%
ACGAAAGGAAGCAAAGCAAT+AGG - tig0028722:37834-37853 None:intergenic 40.0%
TCGATTGATTAGATCGAGTG+CGG + tig0028722:37904-37923 Msa1439160:five_prime_UTR 40.0%
ATATGGTTGTTACTCGATGC+AGG + tig0028722:38150-38169 Msa1439160:exon 40.0%
AAGGTTAAGACTAACCACGT+CGG - tig0028722:38193-38212 None:intergenic 40.0%
TAGAGATAGCAGCTGAACTA+AGG - tig0028722:38240-38259 None:intergenic 40.0%
CTGTAACAGCCTAAAGACAA+AGG - tig0028722:38462-38481 None:intergenic 40.0%
CTTTGTCTTTAGGCTGTTAC+AGG + tig0028722:38460-38479 Msa1439160:intron 40.0%
AAAATGGTATGCGTCGTGAT+TGG + tig0028722:38775-38794 Msa1439160:CDS 40.0%
! TGTCAAGATTTCCCCTGTTT+TGG - tig0028722:38814-38833 None:intergenic 40.0%
!! AAATCTTGACAAGAGCATCC+AGG + tig0028722:38823-38842 Msa1439160:CDS 40.0%
AGTGAAGAAAGGAGCAAGAA+AGG - tig0028722:38866-38885 None:intergenic 40.0%
TTTCTTGCTCCTTTCTTCAC+TGG + tig0028722:38865-38884 Msa1439160:intron 40.0%
ATCTTATGGCCAGTGAAGAA+AGG - tig0028722:38877-38896 None:intergenic 40.0%
! ATCTCTATTTTCAGCCCAGA+TGG + tig0028722:39406-39425 Msa1439160:intron 40.0%
CAATCAAACCACAATCCCTT+TGG - tig0028722:39792-39811 None:intergenic 40.0%
AGCTCATGACTCTCCAAAAT+TGG - tig0028722:39833-39852 None:intergenic 40.0%
!! ATTCCATGACTTTGGAGTAC+AGG + tig0028722:39855-39874 Msa1439160:CDS 40.0%
TTGGAAATTTAGCTGACAGC+GGG + tig0028722:39936-39955 Msa1439160:CDS 40.0%
AACATTTAGGTGCTCTGTGA+TGG - tig0028722:40073-40092 None:intergenic 40.0%
ATGTTTGACTCACTATCAGC+TGG + tig0028722:40088-40107 Msa1439160:three_prime_UTR 40.0%
GTTAATTCAACGGGTGAAAG+AGG + tig0028722:40187-40206 Msa1439160:three_prime_UTR 40.0%
TATATAACGAAGTGGCTGGC+TGG + tig0028722:36568-36587 Msa1439160:five_prime_UTR 45.0%
GGAGGTGAATGAGAATGAGA+AGG - tig0028722:36845-36864 None:intergenic 45.0%
TTCTTCTATGAAGCTGGTGG+AGG - tig0028722:36863-36882 None:intergenic 45.0%
ATTCAAGGTGGTTGAGAGAG+AGG + tig0028722:37187-37206 Msa1439160:intron 45.0%
!! GGAGTTAGTTCTGCTAACCT+TGG + tig0028722:37485-37504 Msa1439160:five_prime_UTR 45.0%
!! GTTAGTTCTGCTAACCTTGG+AGG + tig0028722:37488-37507 Msa1439160:five_prime_UTR 45.0%
GCTTGGATTTGAATCCTCCA+AGG - tig0028722:37505-37524 None:intergenic 45.0%
GCAGCACCTTCCAAAATCAA+CGG + tig0028722:37557-37576 Msa1439160:five_prime_UTR 45.0%
!!! GCAGTTCCGTTGATTTTGGA+AGG - tig0028722:37566-37585 None:intergenic 45.0%
!!! AACCGCAGTTCCGTTGATTT+TGG - tig0028722:37570-37589 None:intergenic 45.0%
AACTGCGGTTACAACTACGA+CGG + tig0028722:37580-37599 Msa1439160:five_prime_UTR 45.0%
TTCTCAGCAGAAGGAAAAGG+TGG - tig0028722:37696-37715 None:intergenic 45.0%
TGCTTCTCAGCAGAAGGAAA+AGG - tig0028722:37699-37718 None:intergenic 45.0%
AGGTGAAATCACAGCAGTCT+AGG - tig0028722:37739-37758 None:intergenic 45.0%
AGAAAGGGAAAACAGGTCGT+AGG - tig0028722:37759-37778 None:intergenic 45.0%
TTGTTACTCGATGCAGGTTG+TGG + tig0028722:38156-38175 Msa1439160:exon 45.0%
AGTAGACACTTGGGTTTCTG+CGG + tig0028722:38746-38765 Msa1439160:CDS 45.0%
GTTTCTGCGGCAGGAAAAAA+TGG + tig0028722:38759-38778 Msa1439160:CDS 45.0%
AAAAACAGTGGCCAGTCACA+AGG - tig0028722:38935-38954 None:intergenic 45.0%
AAAATTCCGGAAGAGGTCAG+AGG + tig0028722:39200-39219 Msa1439160:CDS 45.0%
TCGGCTGAATATATTCGCAC+TGG + tig0028722:39299-39318 Msa1439160:CDS 45.0%
! GAAGTACATTGGGTGGATTC+TGG + tig0028722:39464-39483 Msa1439160:CDS 45.0%
CCACAATCCCTTTGGTTAAG+CGG - tig0028722:39784-39803 None:intergenic 45.0%
TGACTCTCCAAAATTGGCTG+TGG - tig0028722:39827-39846 None:intergenic 45.0%
TGAGCTGCATTCCATGACTT+TGG + tig0028722:39847-39866 Msa1439160:CDS 45.0%
!! CATGACTTTGGAGTACAGGA+GGG + tig0028722:39859-39878 Msa1439160:CDS 45.0%
! ATGATGGTGCCGAGATTGTT+AGG + tig0028722:39990-40009 Msa1439160:CDS 45.0%
! TGATGGTGCCGAGATTGTTA+GGG + tig0028722:39991-40010 Msa1439160:CDS 45.0%
AGTCCTACCCCTAACAATCT+CGG - tig0028722:40002-40021 None:intergenic 45.0%
AGATTGTTAGGGGTAGGACT+GGG + tig0028722:40002-40021 Msa1439160:CDS 45.0%
TTTGACTCACTATCAGCTGG+AGG + tig0028722:40091-40110 Msa1439160:three_prime_UTR 45.0%
GAGCAGAGGAAGAATAGAGT+AGG - tig0028722:40253-40272 None:intergenic 45.0%
TGAATTGGAAGATGGAGCAG+AGG - tig0028722:40267-40286 None:intergenic 45.0%
GTGAGTGAGTGGCACAGAAT+TGG - tig0028722:36597-36616 None:intergenic 50.0%
GGTTACAACTACGACGGTGA+AGG + tig0028722:37586-37605 Msa1439160:five_prime_UTR 50.0%
CACCATCACAACATCACCCA+AGG + tig0028722:37621-37640 Msa1439160:five_prime_UTR 50.0%
GTCCTTGGGTGATGTTGTGA+TGG - tig0028722:37626-37645 None:intergenic 50.0%
! GCAAGAAGCATGCTCCAATC+AGG - tig0028722:37665-37684 None:intergenic 50.0%
TTCCTTCTGCTGAGAAGCAG+TGG + tig0028722:37700-37719 Msa1439160:five_prime_UTR 50.0%
ATCCACTGCTTCTCAGCAGA+AGG - tig0028722:37705-37724 None:intergenic 50.0%
!! TGTTAAGACTGCCGGCTTCT+TGG + tig0028722:38084-38103 Msa1439160:five_prime_UTR 50.0%
CAAAGCCATCACCAAGAAGC+CGG - tig0028722:38098-38117 None:intergenic 50.0%
TGGTTGATCGTTATCCGACG+TGG + tig0028722:38176-38195 Msa1439160:CDS 50.0%
TTGTGCGGGATGCCAAAACA+GGG + tig0028722:38799-38818 Msa1439160:CDS 50.0%
GAATGTGCATGCCTTGTGAC+TGG + tig0028722:38921-38940 Msa1439160:intron 50.0%
ATCTCTCCTCTGACCTCTTC+CGG - tig0028722:39209-39228 None:intergenic 50.0%
TTCTGATCCAGTTGTGGACG+AGG + tig0028722:39247-39266 Msa1439160:CDS 50.0%
CTGTTATCCTCGTCCACAAC+TGG - tig0028722:39257-39276 None:intergenic 50.0%
! GTACATTGGGTGGATTCTGG+AGG + tig0028722:39467-39486 Msa1439160:CDS 50.0%
AACAAGAACGCGAGAAAGCG+AGG - tig0028722:39530-39549 None:intergenic 50.0%
TTATCGGGCCGCTTAACCAA+AGG + tig0028722:39773-39792 Msa1439160:intron 50.0%
TATCGGGCCGCTTAACCAAA+GGG + tig0028722:39774-39793 Msa1439160:intron 50.0%
CCGCTTAACCAAAGGGATTG+TGG + tig0028722:39781-39800 Msa1439160:intron 50.0%
! GAGTGCTCCACAGCCAATTT+TGG + tig0028722:39817-39836 Msa1439160:intron 50.0%
CCTCCTGTACTCCAAAGTCA+TGG - tig0028722:39861-39880 None:intergenic 50.0%
!! CCATGACTTTGGAGTACAGG+AGG + tig0028722:39858-39877 Msa1439160:CDS 50.0%
TTGGAGTACAGGAGGGAATG+TGG + tig0028722:39866-39885 Msa1439160:CDS 50.0%
AGCACCAGAGACAGATGTCA+GGG - tig0028722:39912-39931 None:intergenic 50.0%
!! TCTGTCTCTGGTGCTGACAT+TGG + tig0028722:39917-39936 Msa1439160:CDS 50.0%
! CACTTGCTTCGACTCGATGA+TGG + tig0028722:39974-39993 Msa1439160:CDS 50.0%
! GATGGTGCCGAGATTGTTAG+GGG + tig0028722:39992-40011 Msa1439160:CDS 50.0%
GAGATTGTTAGGGGTAGGAC+TGG + tig0028722:40001-40020 Msa1439160:CDS 50.0%
! TATCAGCTGGAGGTGCAGAT+TGG + tig0028722:40101-40120 Msa1439160:three_prime_UTR 50.0%
TCAACGGGTGAAAGAGGGAT+AGG + tig0028722:40193-40212 Msa1439160:three_prime_UTR 50.0%
CAACGGGTGAAAGAGGGATA+GGG + tig0028722:40194-40213 Msa1439160:three_prime_UTR 50.0%
AAGCCAGCGATGTGAGTGAG+TGG - tig0028722:36608-36627 None:intergenic 55.0%
!! CGGCTTCTTGGTGATGGCTT+TGG + tig0028722:38096-38115 Msa1439160:five_prime_UTR 55.0%
!! TGCGTCGTGATTGGCTTGTG+CGG + tig0028722:38784-38803 Msa1439160:CDS 55.0%
CTTGTGCGGGATGCCAAAAC+AGG + tig0028722:38798-38817 Msa1439160:CDS 55.0%
TGTGCGGGATGCCAAAACAG+GGG + tig0028722:38800-38819 Msa1439160:CDS 55.0%
AGAACGCGAGAAAGCGAGGA+GGG - tig0028722:39526-39545 None:intergenic 55.0%
AAGAACGCGAGAAAGCGAGG+AGG - tig0028722:39527-39546 None:intergenic 55.0%
AGTACAGGAGGGAATGTGGC+AGG + tig0028722:39870-39889 Msa1439160:CDS 55.0%
CAGTCCCTGACATCTGTCTC+TGG + tig0028722:39905-39924 Msa1439160:CDS 55.0%
CAGCACCAGAGACAGATGTC+AGG - tig0028722:39913-39932 None:intergenic 55.0%
GTGCCGAGATTGTTAGGGGT+AGG + tig0028722:39996-40015 Msa1439160:CDS 55.0%
TTGTTAGGGGTAGGACTGGG+TGG + tig0028722:40005-40024 Msa1439160:CDS 55.0%
! GTGCCACTCACTCACATCGC+TGG + tig0028722:36602-36621 Msa1439160:five_prime_UTR 60.0%
!! CACTCACATCGCTGGCTTGC+TGG + tig0028722:36610-36629 Msa1439160:five_prime_UTR 60.0%
!! GACTGCCGGCTTCTTGGTGA+TGG + tig0028722:38090-38109 Msa1439160:five_prime_UTR 60.0%
GACACTTGGGTTTCTGCGGC+AGG + tig0028722:38750-38769 Msa1439160:CDS 60.0%
!! GCGTCGTGATTGGCTTGTGC+GGG + tig0028722:38785-38804 Msa1439160:CDS 60.0%
GAACGCGAGAAAGCGAGGAG+GGG - tig0028722:39525-39544 None:intergenic 60.0%
TTAGGGGTAGGACTGGGTGG+AGG + tig0028722:40008-40027 Msa1439160:CDS 60.0%
CAGCAGGGGGCGGGATAGCC+AGG + tig0028722:36536-36555 Msa1439160:five_prime_UTR 75.0%
CAGGGGGCGGGATAGCCAGG+TGG + tig0028722:36539-36558 Msa1439160:five_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
tig0028722 gene 36528 40413 36528 ID=Msa1439160;Name=Msa1439160
tig0028722 mRNA 36528 40413 36528 ID=Msa1439160-mRNA-1;Parent=Msa1439160;Name=Msa1439160-mRNA-1;_AED=0.03;_eAED=0.03;_QI=863|1|1|1|0.57|0.5|8|326|217
tig0028722 exon 36528 36818 36528 ID=Msa1439160-mRNA-1:exon:11612;Parent=Msa1439160-mRNA-1
tig0028722 exon 37387 37746 37387 ID=Msa1439160-mRNA-1:exon:11613;Parent=Msa1439160-mRNA-1
tig0028722 exon 37854 37966 37854 ID=Msa1439160-mRNA-1:exon:11614;Parent=Msa1439160-mRNA-1
tig0028722 exon 38067 38197 38067 ID=Msa1439160-mRNA-1:exon:11615;Parent=Msa1439160-mRNA-1
tig0028722 exon 38731 38844 38731 ID=Msa1439160-mRNA-1:exon:11616;Parent=Msa1439160-mRNA-1
tig0028722 exon 39160 39328 39160 ID=Msa1439160-mRNA-1:exon:11617;Parent=Msa1439160-mRNA-1
tig0028722 exon 39420 39488 39420 ID=Msa1439160-mRNA-1:exon:11618;Parent=Msa1439160-mRNA-1
tig0028722 exon 39818 40413 39818 ID=Msa1439160-mRNA-1:exon:11619;Parent=Msa1439160-mRNA-1
tig0028722 five_prime_UTR 36528 36818 36528 ID=Msa1439160-mRNA-1:five_prime_utr;Parent=Msa1439160-mRNA-1
tig0028722 five_prime_UTR 37387 37746 37387 ID=Msa1439160-mRNA-1:five_prime_utr;Parent=Msa1439160-mRNA-1
tig0028722 five_prime_UTR 37854 37966 37854 ID=Msa1439160-mRNA-1:five_prime_utr;Parent=Msa1439160-mRNA-1
tig0028722 five_prime_UTR 38067 38165 38067 ID=Msa1439160-mRNA-1:five_prime_utr;Parent=Msa1439160-mRNA-1
tig0028722 CDS 38166 38197 38166 ID=Msa1439160-mRNA-1:cds;Parent=Msa1439160-mRNA-1
tig0028722 CDS 38731 38844 38731 ID=Msa1439160-mRNA-1:cds;Parent=Msa1439160-mRNA-1
tig0028722 CDS 39160 39328 39160 ID=Msa1439160-mRNA-1:cds;Parent=Msa1439160-mRNA-1
tig0028722 CDS 39420 39488 39420 ID=Msa1439160-mRNA-1:cds;Parent=Msa1439160-mRNA-1
tig0028722 CDS 39818 40087 39818 ID=Msa1439160-mRNA-1:cds;Parent=Msa1439160-mRNA-1
tig0028722 three_prime_UTR 40088 40413 40088 ID=Msa1439160-mRNA-1:three_prime_utr;Parent=Msa1439160-mRNA-1
Gene Sequence

>Msa1439160

ATGCAGGTTGTGGTTGATCGTTATCCGACGTGGGGTGAAGTTGTTCAAGTAGACACTTGGGTTTCTGCGGCAGGAAAAAATGGTATGCGTCGTGATTGGCTTGTGCGGGATGCCAAAACAGGGGAAATCTTGACAAGAGCATCCAGTGTTTGGGTCATGATGAATAAACTGACAAGGAAACTATCTAAAATTCCGGAAGAGGTCAGAGGAGAGATAGAGCATTATTTTGTTAATTCTGATCCAGTTGTGGACGAGGATAACAGAAAACTAACTAAACTTGATGATTCGGCTGAATATATTCGCACTGGTTTAAGTCCCAGATGGAATGATCTAGATGTTAATCAGCATGTTAATAATGTGAAGTACATTGGGTGGATTCTGGAGAGTGCTCCACAGCCAATTTTGGAGAGTCATGAGCTGCATTCCATGACTTTGGAGTACAGGAGGGAATGTGGCAGGAACAGTGTACTTCAGTCCCTGACATCTGTCTCTGGTGCTGACATTGGAAATTTAGCTGACAGCGGGTTTGTTGAATGCAAACACTTGCTTCGACTCGATGATGGTGCCGAGATTGTTAGGGGTAGGACTGGGTGGAGGCCAAAACCTATCAACAATTTTGATGTTCTGAATCAGTTTCCATCACAGAGCACCTAA
Protein sequence

>Msa1439160

MQVVVDRYPTWGEVVQVDTWVSAAGKNGMRRDWLVRDAKTGEILTRASSVWVMMNKLTRKLSKIPEEVRGEIEHYFVNSDPVVDEDNRKLTKLDDSAEYIRTGLSPRWNDLDVNQHVNNVKYIGWILESAPQPILESHELHSMTLEYRRECGRNSVLQSLTSVSGADIGNLADSGFVECKHLLRLDDGAEIVRGRTGWRPKPINNFDVLNQFPSQST*