AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0112800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0112800 MtrunA17_Chr1g0181121 98.532 681 10 0 28 708 1 681 0.0 1399
Msa0112800 MtrunA17_Chr2g0326871 60.169 708 265 4 7 708 7 703 0.0 897
Msa0112800 MtrunA17_Chr4g0028861 65.610 631 201 3 1 629 66 682 0.0 863
Msa0112800 MtrunA17_Chr2g0305771 53.997 663 288 6 58 708 30 687 0.0 737
Msa0112800 MtrunA17_Chr3g0139071 53.765 664 289 6 58 706 60 720 0.0 724
Msa0112800 MtrunA17_Chr7g0228181 49.054 687 315 6 46 708 5 680 0.0 692
Msa0112800 MtrunA17_Chr4g0073941 51.729 665 300 10 51 708 78 728 0.0 689
Msa0112800 MtrunA17_Chr1g0158861 50.215 699 312 10 18 708 44 714 0.0 686
Msa0112800 MtrunA17_Chr3g0126541 51.347 668 299 10 48 708 63 711 0.0 680
Msa0112800 MtrunA17_Chr3g0125651 50.307 652 308 4 71 706 36 687 0.0 678
Msa0112800 MtrunA17_Chr4g0001481 49.711 692 319 14 31 706 59 737 0.0 641
Msa0112800 MtrunA17_Chr7g0275581 49.163 657 300 11 7 638 7 654 0.0 632
Msa0112800 MtrunA17_Chr8g0347121 45.735 680 309 11 1 637 1 663 0.0 598
Msa0112800 MtrunA17_Chr8g0360821 35.993 614 358 13 73 664 64 664 1.68e-117 367
Msa0112800 MtrunA17_Chr6g0480851 36.498 611 363 10 47 641 160 761 6.94e-111 353
Msa0112800 MtrunA17_Chr6g0480781 35.959 584 345 15 71 636 169 741 1.08e-110 352
Msa0112800 MtrunA17_Chr6g0481021 36.573 607 353 12 47 631 99 695 5.69e-109 346
Msa0112800 MtrunA17_Chr6g0480671 38.966 580 315 12 71 627 193 756 1.61e-108 347
Msa0112800 MtrunA17_Chr7g0218341 35.788 584 353 10 71 638 109 686 8.81e-105 335
Msa0112800 MtrunA17_Chr2g0318561 37.307 453 265 8 186 625 2 448 3.46e-93 298
Msa0112800 MtrunA17_Chr6g0480741 33.390 584 353 12 71 634 115 682 3.02e-89 293
Msa0112800 MtrunA17_Chr6g0480681 30.628 653 390 14 29 635 73 708 3.98e-81 273
Msa0112800 MtrunA17_Chr2g0326861 57.513 193 82 0 145 337 14 206 1.42e-77 248
Msa0112800 MtrunA17_Chr5g0395421 37.784 352 191 8 268 605 18 355 6.48e-55 192
Msa0112800 MtrunA17_Chr2g0326851 50.000 142 60 2 567 708 3 133 1.70e-40 144
Msa0112800 MtrunA17_Chr6g0480771 35.714 252 139 7 388 626 12 253 3.74e-34 132
Msa0112800 MtrunA17_Chr6g0480841 39.899 198 99 5 442 626 2 192 4.78e-32 124
Msa0112800 MtrunA17_Chr5g0395411 33.000 200 120 3 71 269 172 358 1.33e-31 127
Msa0112800 MtrunA17_Chr6g0480751 37.313 134 83 1 71 203 98 231 4.10e-23 98.6
Msa0112800 MtrunA17_Chr6g0480791 36.885 122 75 2 71 190 172 293 1.18e-19 90.1
Msa0112800 MtrunA17_Chr4g0063141 21.605 486 282 16 70 545 45 441 2.01e-18 90.1
Msa0112800 MtrunA17_Chr2g0277651 20.619 485 305 12 122 599 106 517 2.92e-16 83.2
Msa0112800 MtrunA17_Chr6g0480821 28.824 170 112 6 187 352 25 189 3.70e-16 78.2
Msa0112800 MtrunA17_Chr5g0434371 26.257 179 129 2 342 520 282 457 3.79e-15 79.7
Msa0112800 MtrunA17_Chr2g0318551 31.609 174 102 8 61 231 54 213 4.29e-15 75.1
Msa0112800 MtrunA17_Chr3g0138671 22.831 219 165 3 341 559 241 455 1.45e-13 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0112800 AT2G28260.1 59.744 703 253 2 6 708 6 678 0.0 874
Msa0112800 AT2G28260.2 62.253 657 221 1 52 708 9 638 0.0 867
Msa0112800 AT2G24610.1 50.759 725 326 9 8 708 8 725 0.0 715
Msa0112800 AT2G24610.2 50.761 723 325 9 10 708 25 740 0.0 712
Msa0112800 AT4G30360.1 52.160 648 299 5 71 708 71 717 0.0 702
Msa0112800 AT2G23980.9 49.493 691 334 8 29 708 59 745 0.0 694
Msa0112800 AT2G23980.10 49.493 691 334 8 29 708 59 745 0.0 694
Msa0112800 AT2G23980.1 49.493 691 334 8 29 708 59 745 0.0 694
Msa0112800 AT2G23980.4 49.493 691 334 8 29 708 59 745 0.0 694
Msa0112800 AT2G23980.5 49.493 691 334 8 29 708 59 745 0.0 694
Msa0112800 AT2G23980.6 49.493 691 334 8 29 708 32 718 0.0 694
Msa0112800 AT5G57940.1 51.416 671 293 8 51 708 67 717 0.0 688
Msa0112800 AT5G57940.2 51.416 671 293 8 51 708 67 717 0.0 688
Msa0112800 AT5G57940.3 51.416 671 293 8 51 708 60 710 0.0 688
Msa0112800 AT4G30560.1 49.569 696 323 8 23 708 53 730 0.0 687
Msa0112800 AT4G30560.4 49.569 696 323 8 23 708 53 730 0.0 687
Msa0112800 AT4G30560.5 49.569 696 323 8 23 708 27 704 0.0 687
Msa0112800 AT4G30560.2 49.569 696 323 8 23 708 27 704 0.0 687
Msa0112800 AT4G30560.3 49.569 696 323 8 23 708 27 704 0.0 687
Msa0112800 AT1G15990.1 49.211 697 327 10 34 708 47 738 0.0 685
Msa0112800 AT2G23980.3 48.039 714 333 9 29 708 59 768 0.0 681
Msa0112800 AT2G23980.2 48.039 714 333 9 29 708 59 768 0.0 681
Msa0112800 AT2G23980.8 48.039 714 333 9 29 708 59 768 0.0 681
Msa0112800 AT2G23980.7 48.039 714 333 9 29 708 59 768 0.0 681
Msa0112800 AT1G19780.1 51.198 668 306 9 55 705 80 744 0.0 680
Msa0112800 AT5G53130.1 51.473 645 293 8 71 708 84 715 0.0 669
Msa0112800 AT3G48010.1 53.833 600 261 6 47 636 26 619 0.0 667
Msa0112800 AT5G14870.1 47.833 669 312 6 71 708 40 702 0.0 660
Msa0112800 AT1G01340.1 50.156 642 309 6 71 706 63 699 0.0 659
Msa0112800 AT1G01340.2 50.156 642 309 6 71 706 68 704 0.0 658
Msa0112800 AT4G01010.1 49.851 672 316 8 40 706 37 692 0.0 652
Msa0112800 AT4G01010.2 49.851 672 316 8 40 706 37 692 0.0 652
Msa0112800 AT2G46430.1 48.457 648 309 10 71 708 72 704 0.0 608
Msa0112800 AT2G46430.2 48.457 648 309 10 71 708 72 704 0.0 608
Msa0112800 AT1G19780.2 51.963 535 239 7 186 705 2 533 0.0 547
Msa0112800 AT2G46440.1 45.250 579 300 8 82 653 40 608 3.44e-162 481
Msa0112800 AT2G46450.4 40.989 566 314 8 82 639 40 593 7.13e-149 448
Msa0112800 AT2G46450.1 40.989 566 314 8 82 639 40 593 7.13e-149 448
Msa0112800 AT2G46450.3 39.292 565 302 9 82 639 27 557 1.63e-136 415
Msa0112800 AT2G46450.2 39.292 565 302 9 82 639 40 570 3.54e-136 414
Msa0112800 AT2G46440.2 49.199 437 209 6 223 653 1 430 6.45e-129 389
Msa0112800 AT5G54250.2 35.131 612 374 10 71 664 79 685 3.41e-114 360
Msa0112800 AT5G54250.1 35.131 612 374 10 71 664 79 685 3.41e-114 360
Msa0112800 AT5G54250.4 35.131 612 374 10 71 664 79 685 3.41e-114 360
Msa0112800 AT3G17690.1 34.053 602 370 14 52 638 139 728 2.71e-111 353
Msa0112800 AT5G54250.3 34.477 612 373 11 71 664 79 680 1.11e-108 345
Msa0112800 AT3G17700.1 36.752 585 343 14 52 621 173 745 1.52e-108 347
Msa0112800 AT5G15410.1 35.714 574 343 11 67 621 111 677 2.59e-105 337
Msa0112800 AT5G15410.2 35.714 574 343 11 67 621 110 676 3.13e-105 337
Msa0112800 AT3G17690.2 33.925 507 318 9 51 552 138 632 6.62e-98 315
Msa0112800 AT3G17700.2 36.364 506 306 10 52 553 173 666 6.22e-97 314
Msa0112800 AT3G17690.3 32.115 383 250 7 52 429 139 516 1.02e-64 224
Msa0112800 AT3G02850.3 22.323 439 267 12 82 520 85 449 6.03e-18 88.6
Msa0112800 AT3G02850.2 22.779 439 265 12 82 520 85 449 1.92e-17 87.4
Msa0112800 AT3G02850.1 22.779 439 265 13 82 520 85 449 3.51e-17 86.7
Msa0112800 AT4G22200.2 19.484 426 273 8 121 545 107 463 4.25e-14 76.3
Msa0112800 AT4G22200.1 19.484 426 273 8 121 545 107 463 4.95e-14 76.3
Msa0112800 AT5G37500.4 25.683 183 133 2 342 524 257 436 8.57e-14 75.5
Msa0112800 AT5G37500.3 25.543 184 134 2 342 525 203 383 8.71e-14 75.5
Msa0112800 AT5G37500.2 25.683 183 133 2 342 524 257 436 1.36e-13 75.1
Msa0112800 AT5G37500.1 25.683 183 133 2 342 524 257 436 1.41e-13 75.1

Find 127 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCCTTCATCTCGTGTTTC+TGG 0.112326 1_3:-28335250 Msa0112800:CDS
ACATTATTCATATCTATATT+AGG 0.216780 1_3:-28335291 Msa0112800:CDS
GAAAACTAGTAGGTAGTTTA+AGG 0.218586 1_3:-28332203 Msa0112800:CDS
GCTGTGATTGTTGCAATTCT+TGG 0.224686 1_3:-28333794 Msa0112800:CDS
CCAAGTATTATTATTGTCTT+TGG 0.233035 1_3:-28333981 Msa0112800:CDS
TTAAGCCAAGCATTCCTATT+TGG 0.253153 1_3:+28334108 None:intergenic
TCAAGTTATTCAATGTATTC+TGG 0.255289 1_3:-28332225 Msa0112800:CDS
CCTCAATAAGTACTGGAAAA+TGG 0.258411 1_3:+28334707 None:intergenic
ATGTCAAATGTGAAATATTA+TGG 0.260963 1_3:-28332297 Msa0112800:CDS
GAGGACCTAGAATAAATTTA+TGG 0.262159 1_3:-28335465 Msa0112800:CDS
TTACTTGTCTAACTAGATTA+AGG 0.272354 1_3:+28333248 None:intergenic
TTGTTCCATAAATTTATTCT+AGG 0.278851 1_3:+28335460 None:intergenic
TGATGAATTTGCAGCTCTTC+AGG 0.284395 1_3:-28333860 Msa0112800:intron
TTGATATGCTTGGTACATTA+AGG 0.286363 1_3:-28332149 Msa0112800:CDS
ATTGAATAACTTGATCCTTT+TGG 0.295632 1_3:+28332234 None:intergenic
AACAGTCACTTCTTTCAGTT+TGG 0.303420 1_3:-28334054 Msa0112800:CDS
TGCATCAAACTACATGAAAA+AGG 0.316525 1_3:-28335194 Msa0112800:CDS
CTTGTCTTTCAATTGATAAC+AGG 0.322976 1_3:+28334211 None:intergenic
AGCAGTCGGGCGAGGGTCTA+AGG 0.324635 1_3:+28332569 None:intergenic
GCTGTAATTCCAAATATAAA+AGG 0.326602 1_3:-28334771 Msa0112800:CDS
TCATTAAAATCTGGTTCAAT+AGG 0.330248 1_3:+28332123 None:intergenic
GACAATCAAGATAATGATAT+TGG 0.332462 1_3:+28334145 None:intergenic
TATAATGCTAGGTACTCATT+TGG 0.340611 1_3:-28334794 Msa0112800:intron
ACCTTCAATCGACCACTATA+CGG 0.343757 1_3:-28333462 Msa0112800:CDS
GGTGGGGATTGAGGAATCTA+AGG 0.352167 1_3:-28333960 Msa0112800:intron
GAAGCTTAAGTGATCCAAAT+AGG 0.355960 1_3:-28334122 Msa0112800:CDS
CTGCAACATGTGTACTTGTT+AGG 0.369323 1_3:+28333828 None:intergenic
GACAAATGAGGTTAAGGAAA+AGG 0.372638 1_3:-28332334 Msa0112800:CDS
GAAAGAGACAAATGAGGTTA+AGG 0.381363 1_3:-28332340 Msa0112800:CDS
TTGTTTGATTTCATTGTTTG+TGG 0.391370 1_3:-28335422 Msa0112800:CDS
TTATACAAGCAGCTTGGTTT+CGG 0.394586 1_3:-28332374 Msa0112800:CDS
GACGCCATGACCAATTTAGA+TGG 0.405101 1_3:-28333354 Msa0112800:CDS
TCGCGAACTATGCAAGTTCC+TGG 0.405890 1_3:+28332732 None:intergenic
TCAGTTGTTTGCTATGTAAC+CGG 0.409128 1_3:+28332448 None:intergenic
CATCATTACTCCACTTGCCT+TGG 0.413407 1_3:+28334650 None:intergenic
ATGTCACGTCTTAGATCAAC+AGG 0.421675 1_3:+28333286 None:intergenic
TGCTAGTCATCATTAAAATC+TGG 0.437031 1_3:+28332114 None:intergenic
GTATTATTATTGTCTTTGGT+GGG 0.441309 1_3:-28333977 Msa0112800:CDS
AAATTTATTCTAGGTCCTCT+AGG 0.441726 1_3:+28335469 None:intergenic
AGTCCTCTTCCATTTGATGA+TGG 0.444375 1_3:+28335544 None:intergenic
ATTGTCTTTGGTGGGGATTG+AGG 0.450681 1_3:-28333969 Msa0112800:CDS
ATATTTCTCTGCACCTCTGC+AGG 0.451022 1_3:-28332259 Msa0112800:CDS
ACGACCAACGGTGGTAGAAC+TGG 0.455245 1_3:-28332654 Msa0112800:CDS
GAGTAGCTACCCATCTAAAT+TGG 0.455345 1_3:+28333344 None:intergenic
CTTAAATCAAGCAATCTATC+AGG 0.455643 1_3:-28334090 Msa0112800:CDS
AGTATTATTATTGTCTTTGG+TGG 0.455701 1_3:-28333978 Msa0112800:CDS
GGAAACACAGCAGTCGGGCG+AGG 0.464302 1_3:+28332561 None:intergenic
TCATCTCGTGTTTCTGGTCG+AGG 0.464605 1_3:-28335244 Msa0112800:CDS
GATTGTTGCAATTCTTGGAT+TGG 0.464760 1_3:-28333789 Msa0112800:CDS
CTCACTGTGCGAGTTGATGA+AGG 0.471556 1_3:+28332540 None:intergenic
CAAGGCAAGTGGAGTAATGA+TGG 0.472612 1_3:-28334649 Msa0112800:CDS
ATGTATTCTGGAAAACTAGT+AGG 0.473124 1_3:-28332213 Msa0112800:CDS
GTGATCCAAATAGGAATGCT+TGG 0.473435 1_3:-28334113 Msa0112800:CDS
GTCGCCTGAGCTTATTCTGC+AGG 0.473910 1_3:+28332620 None:intergenic
ATAATGCTAGGTACTCATTT+GGG 0.476442 1_3:-28334793 Msa0112800:intron
GTAGTTTGATGCAATCTTTG+AGG 0.478756 1_3:+28335204 None:intergenic
AAGTGTATTGATATGTCAAT+TGG 0.485639 1_3:-28335361 Msa0112800:CDS
ATAAACAAGCCATCATCAAA+TGG 0.489789 1_3:-28335553 Msa0112800:CDS
TTGAAAGACAAGATGAATGT+TGG 0.490493 1_3:-28334200 Msa0112800:CDS
ATGAAGAGCATTTCGTCGAC+AGG 0.493141 1_3:+28332702 None:intergenic
GATACAACCTCAATAAGTAC+TGG 0.495573 1_3:+28334700 None:intergenic
TGCTACAAACTTTAAATCCT+CGG 0.498284 1_3:+28332482 None:intergenic
GCCGTATAGTGGTCGATTGA+AGG 0.500147 1_3:+28333461 None:intergenic
CTGTAATTCCAAATATAAAA+GGG 0.501630 1_3:-28334770 Msa0112800:CDS
CCAAAGACAATAATAATACT+TGG 0.503297 1_3:+28333981 None:intergenic
GCACCTCTGCAGGTGCCAAA+AGG 0.504350 1_3:-28332249 Msa0112800:CDS
CAGGAATATACCTGAGGAAG+TGG 0.505032 1_3:+28335142 None:intergenic
TCAGGTAACGAGGGAGCTGC+CGG 0.505935 1_3:+28333389 None:intergenic
GAAATGCTCTTCATCGTTCG+AGG 0.508603 1_3:-28332693 Msa0112800:CDS
ATCAAGGCATGTAGTTCGCT+TGG 0.510564 1_3:-28334733 Msa0112800:CDS
TCATCCATCTGATCAAATAG+TGG 0.519800 1_3:+28332804 None:intergenic
CAGAAAGATGGATGCATCAC+CGG 0.521281 1_3:-28333408 Msa0112800:CDS
TTAGATTCTATTCACATCAG+TGG 0.525876 1_3:-28332416 Msa0112800:CDS
CCACTCTTCCAGCCGTATAG+TGG 0.529262 1_3:+28333450 None:intergenic
TGCAGCACTACCACTTCCTC+AGG 0.529548 1_3:-28335152 Msa0112800:intron
ACTGGTAAGTAAAAGAAAAG+TGG 0.529988 1_3:+28335397 None:intergenic
TCAATCGACCACTATACGGC+TGG 0.530054 1_3:-28333458 Msa0112800:CDS
TATTATTATTGTCTTTGGTG+GGG 0.530464 1_3:-28333976 Msa0112800:CDS
ATTCACATCAGTGGAGGACA+TGG 0.530701 1_3:-28332407 Msa0112800:CDS
TTGGTTATCTTAGATGATAG+AGG 0.537065 1_3:+28334669 None:intergenic
GGTACCACTATTTGATCAGA+TGG 0.540996 1_3:-28332808 Msa0112800:CDS
CTGTTATATCAAGAGTCGTA+AGG 0.542793 1_3:-28335935 Msa0112800:intron
GTTCCTGGAGTGCATAGAGT+TGG 0.542992 1_3:+28332747 None:intergenic
ATGAAGGAAACACAGCAGTC+GGG 0.545943 1_3:+28332556 None:intergenic
AATTTATTCTAGGTCCTCTA+GGG 0.546116 1_3:+28335470 None:intergenic
CGACCAGAAACACGAGATGA+AGG 0.546693 1_3:+28335247 None:intergenic
CCACTATACGGCTGGAAGAG+TGG 0.546695 1_3:-28333450 Msa0112800:CDS
TATTTGATCAGATGGATGAC+AGG 0.547337 1_3:-28332800 Msa0112800:CDS
GATGAAGGAAACACAGCAGT+CGG 0.548177 1_3:+28332555 None:intergenic
TTAGATTCTTGCACGACCAA+CGG 0.554372 1_3:-28332666 Msa0112800:CDS
GAAGGACAGATACAGAAAGA+TGG 0.556176 1_3:-28333420 Msa0112800:CDS
GAGAATGAAAGAGACAAATG+AGG 0.556459 1_3:-28332346 Msa0112800:CDS
AAATGCTTCGACTTCTGTGA+TGG 0.558128 1_3:+28332515 None:intergenic
TGGAAGAGTGGCGAATAAGA+AGG 0.567637 1_3:-28333438 Msa0112800:CDS
GTCTAACTAGATTAAGGCAA+AGG 0.567848 1_3:+28333254 None:intergenic
AACACCTGCAGAATAAGCTC+AGG 0.570561 1_3:-28332624 Msa0112800:CDS
AAGCCATCATCAAATGGAAG+AGG 0.574296 1_3:-28335547 Msa0112800:CDS
CTAGTAGGTAGTTTAAGGAG+AGG 0.578326 1_3:-28332198 Msa0112800:CDS
GAAACACAGCAGTCGGGCGA+GGG 0.579139 1_3:+28332562 None:intergenic
TTGTAGCAGCACAATTCCGC+CGG 0.583306 1_3:-28332467 Msa0112800:CDS
CTACCCATCTAAATTGGTCA+TGG 0.585160 1_3:+28333350 None:intergenic
ACGCCATGACCAATTTAGAT+GGG 0.585828 1_3:-28333353 Msa0112800:CDS
AAACCAACTCTATGCACTCC+AGG 0.588925 1_3:-28332750 Msa0112800:CDS
GATTCTATTCACATCAGTGG+AGG 0.595512 1_3:-28332413 Msa0112800:CDS
GGGCGAGGGTCTAAGGCCCA+TGG 0.598732 1_3:+28332576 None:intergenic
AAATGAGTGCATACGAGATG+AGG 0.599147 1_3:-28335603 Msa0112800:CDS
GATGCTGAACTTGATATGCT+TGG 0.599745 1_3:-28332159 Msa0112800:CDS
AGCTACTCGTGGAGTTGACG+AGG 0.602552 1_3:-28333329 Msa0112800:CDS
TGTTAGATGCAATATGTGAA+AGG 0.604722 1_3:-28332776 Msa0112800:CDS
AGCATTTGCACTCATTGCCG+AGG 0.611919 1_3:-28332499 Msa0112800:CDS
CTGATGTGAATAGAATCTAA+AGG 0.613822 1_3:+28332419 None:intergenic
TCTAAGATAACCAAGGCAAG+TGG 0.615553 1_3:-28334660 Msa0112800:CDS
GCAGCTCCCTCGTTACCTGA+AGG 0.626636 1_3:-28333386 Msa0112800:CDS
AGATAATGATATTGGCAATG+TGG 0.627476 1_3:+28334153 None:intergenic
TTTAGATGGGTAGCTACTCG+TGG 0.627869 1_3:-28333340 Msa0112800:CDS
GGTCAGAAATGATTGCATCA+AGG 0.631571 1_3:-28334749 Msa0112800:CDS
ATGAGGAGTGAATACAAGCA+TGG 0.634357 1_3:-28335586 Msa0112800:CDS
ATAAGCTCAGGCGACTTCTG+CGG 0.636175 1_3:-28332612 Msa0112800:CDS
TAACTAGATTAAGGCAAAGG+TGG 0.642852 1_3:+28333257 None:intergenic
GTTGTTTGCTATGTAACCGG+CGG 0.650163 1_3:+28332451 None:intergenic
AAAACCAGTTCTACCACCGT+TGG 0.651859 1_3:+28332650 None:intergenic
AGAAGGTGAAGAAAGTCACA+TGG 0.653076 1_3:-28335626 Msa0112800:CDS
TTCACATCAGTGGAGGACAT+GGG 0.654472 1_3:-28332406 Msa0112800:CDS
TCTATCATCTAAGATAACCA+AGG 0.655489 1_3:-28334667 Msa0112800:CDS
TGAAGAGCATTTCGTCGACA+GGG 0.658976 1_3:+28332703 None:intergenic
GGAACTTGCATAGTTCGCGA+AGG 0.669380 1_3:-28332729 Msa0112800:CDS
GATTCTTGCACGACCAACGG+TGG 0.711618 1_3:-28332663 Msa0112800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTAATTACAATGTTAAA+GGG + chr1_3:28332172-28332191 None:intergenic 10.0%
!!! TTTTTTTACAAAAATATGTT+TGG - chr1_3:28332356-28332375 Msa0112800:CDS 10.0%
!! CATATTAATTACAATGTTAA+AGG + chr1_3:28332173-28332192 None:intergenic 15.0%
!! ACATTATTCATATCTATATT+AGG - chr1_3:28332777-28332796 Msa0112800:CDS 15.0%
!! GATAATTAAAAATGAATATC+AGG + chr1_3:28332948-28332967 None:intergenic 15.0%
!! AATAAGCTAAAAACAATTTA+TGG + chr1_3:28333656-28333675 None:intergenic 15.0%
!! TATGAAAATAAAACAACTTA+TGG - chr1_3:28334513-28334532 Msa0112800:intron 15.0%
!! CAATAAAAGAAATAATGTAA+CGG + chr1_3:28334578-28334597 None:intergenic 15.0%
!! TTGTTCCATAAATTTATTCT+AGG + chr1_3:28332611-28332630 None:intergenic 20.0%
!!! TATTTTAATTATGCGAAGAA+CGG + chr1_3:28333208-28333227 None:intergenic 20.0%
!! GATAATTCATATATAATGCT+AGG - chr1_3:28333263-28333282 Msa0112800:CDS 20.0%
!! CTGTAATTCCAAATATAAAA+GGG - chr1_3:28333298-28333317 Msa0112800:CDS 20.0%
!!! GTATTACGAAAAACATTTTT+CGG + chr1_3:28333514-28333533 None:intergenic 20.0%
!!! ACCTTTTCTTTATTTCAAAA+AGG - chr1_3:28333815-28333834 Msa0112800:CDS 20.0%
!!! ACCTTTTTGAAATAAAGAAA+AGG + chr1_3:28333819-28333838 None:intergenic 20.0%
!!! CTTTATTTCAAAAAGGTTTT+AGG - chr1_3:28333822-28333841 Msa0112800:CDS 20.0%
!! AGAATTGGTTAGAATAAATA+TGG + chr1_3:28334187-28334206 None:intergenic 20.0%
!! ATTCACTAATTTAGATTAGA+CGG - chr1_3:28334921-28334940 Msa0112800:intron 20.0%
!! TTCTAAATCAGTAAAAATTG+TGG - chr1_3:28334996-28335015 Msa0112800:intron 20.0%
!! AAAAATTATGTGATTAAGAG+TGG + chr1_3:28335131-28335150 None:intergenic 20.0%
!!! ATTAAGAATGAAGTACTAAT+TGG - chr1_3:28335223-28335242 Msa0112800:CDS 20.0%
!! ATGTCAAATGTGAAATATTA+TGG - chr1_3:28335771-28335790 Msa0112800:intron 20.0%
! GAAAAAGAGAAATTACAAGA+AGG - chr1_3:28332425-28332444 Msa0112800:CDS 25.0%
!! TTGTTTGATTTCATTGTTTG+TGG - chr1_3:28332646-28332665 Msa0112800:CDS 25.0%
!! AAGTGTATTGATATGTCAAT+TGG - chr1_3:28332707-28332726 Msa0112800:CDS 25.0%
!! ACTACATGAAAAAGGAATTT+TGG - chr1_3:28332882-28332901 Msa0112800:intron 25.0%
! TATGTTTAAATGTTCTGTGT+TGG - chr1_3:28333143-28333162 Msa0112800:intron 25.0%
!!! AATTTTATGGTTTGCAAACA+TGG - chr1_3:28333230-28333249 Msa0112800:intron 25.0%
! GCTGTAATTCCAAATATAAA+AGG - chr1_3:28333297-28333316 Msa0112800:CDS 25.0%
!! ATTTCTGACCCTTTTATATT+TGG + chr1_3:28333309-28333328 None:intergenic 25.0%
! GACAATCAAGATAATGATAT+TGG + chr1_3:28333926-28333945 None:intergenic 25.0%
! CCAAAGACAATAATAATACT+TGG + chr1_3:28334090-28334109 None:intergenic 25.0%
! CCAAGTATTATTATTGTCTT+TGG - chr1_3:28334087-28334106 Msa0112800:CDS 25.0%
! AGTATTATTATTGTCTTTGG+TGG - chr1_3:28334090-28334109 Msa0112800:CDS 25.0%
! GTATTATTATTGTCTTTGGT+GGG - chr1_3:28334091-28334110 Msa0112800:CDS 25.0%
! TATTATTATTGTCTTTGGTG+GGG - chr1_3:28334092-28334111 Msa0112800:CDS 25.0%
! TTACTTGTCTAACTAGATTA+AGG + chr1_3:28334823-28334842 None:intergenic 25.0%
!! GCTTTTTATTACACAGATTA+TGG - chr1_3:28334877-28334896 Msa0112800:intron 25.0%
! ATATTCCAGAAATTCATTCA+TGG - chr1_3:28335031-28335050 Msa0112800:intron 25.0%
!! ATTGAATAACTTGATCCTTT+TGG + chr1_3:28335837-28335856 None:intergenic 25.0%
! TCAAGTTATTCAATGTATTC+TGG - chr1_3:28335843-28335862 Msa0112800:intron 25.0%
!! TCATTAAAATCTGGTTCAAT+AGG + chr1_3:28335948-28335967 None:intergenic 25.0%
ATAAACAAGCCATCATCAAA+TGG - chr1_3:28332515-28332534 Msa0112800:CDS 30.0%
AATTTATTCTAGGTCCTCTA+GGG + chr1_3:28332601-28332620 None:intergenic 30.0%
AAATTTATTCTAGGTCCTCT+AGG + chr1_3:28332602-28332621 None:intergenic 30.0%
GAGGACCTAGAATAAATTTA+TGG - chr1_3:28332603-28332622 Msa0112800:CDS 30.0%
ACTGGTAAGTAAAAGAAAAG+TGG + chr1_3:28332674-28332693 None:intergenic 30.0%
TGCATCAAACTACATGAAAA+AGG - chr1_3:28332874-28332893 Msa0112800:intron 30.0%
TATAATGCTAGGTACTCATT+TGG - chr1_3:28333274-28333293 Msa0112800:CDS 30.0%
ATAATGCTAGGTACTCATTT+GGG - chr1_3:28333275-28333294 Msa0112800:CDS 30.0%
TTGGTTATCTTAGATGATAG+AGG + chr1_3:28333402-28333421 None:intergenic 30.0%
! TCTATCATCTAAGATAACCA+AGG - chr1_3:28333401-28333420 Msa0112800:CDS 30.0%
! AGTAATGATGGAAAAAGCTT+GGG - chr1_3:28333431-28333450 Msa0112800:CDS 30.0%
CTTATTTCCGTAAGTTCTAT+AGG - chr1_3:28333670-28333689 Msa0112800:intron 30.0%
AAGCTATCCTATAGAACTTA+CGG + chr1_3:28333680-28333699 None:intergenic 30.0%
TAAGAAATGAATCAGAAGCA+AGG + chr1_3:28333795-28333814 None:intergenic 30.0%
!!! AAAAGGTTTTAGGATCAACT+TGG - chr1_3:28333832-28333851 Msa0112800:CDS 30.0%
! CTTGTCTTTCAATTGATAAC+AGG + chr1_3:28333860-28333879 None:intergenic 30.0%
TTGAAAGACAAGATGAATGT+TGG - chr1_3:28333868-28333887 Msa0112800:intron 30.0%
AGATAATGATATTGGCAATG+TGG + chr1_3:28333918-28333937 None:intergenic 30.0%
CTTAAATCAAGCAATCTATC+AGG - chr1_3:28333978-28333997 Msa0112800:CDS 30.0%
! TTTTTATGATGCTGTGACTT+TGG - chr1_3:28334040-28334059 Msa0112800:CDS 30.0%
CTGCAAATTCATCATAGAAT+TGG + chr1_3:28334202-28334221 None:intergenic 30.0%
!! AAAAAAAAGTGTCACACACA+TGG + chr1_3:28335163-28335182 None:intergenic 30.0%
TGTTAGATGCAATATGTGAA+AGG - chr1_3:28335292-28335311 Msa0112800:CDS 30.0%
TGCTACAAACTTTAAATCCT+CGG + chr1_3:28335589-28335608 None:intergenic 30.0%
CTGATGTGAATAGAATCTAA+AGG + chr1_3:28335652-28335671 None:intergenic 30.0%
TTAGATTCTATTCACATCAG+TGG - chr1_3:28335652-28335671 Msa0112800:CDS 30.0%
! ATGTATTCTGGAAAACTAGT+AGG - chr1_3:28335855-28335874 Msa0112800:intron 30.0%
GAAAACTAGTAGGTAGTTTA+AGG - chr1_3:28335865-28335884 Msa0112800:intron 30.0%
TTGATATGCTTGGTACATTA+AGG - chr1_3:28335919-28335938 Msa0112800:intron 30.0%
TGCTAGTCATCATTAAAATC+TGG + chr1_3:28335957-28335976 None:intergenic 30.0%
!! GATTTTAATGATGACTAGCA+TGG - chr1_3:28335957-28335976 Msa0112800:exon 30.0%
CTGTTATATCAAGAGTCGTA+AGG - chr1_3:28332133-28332152 Msa0112800:CDS 35.0%
ATGTATCAACTCATACCACT+AGG + chr1_3:28332282-28332301 None:intergenic 35.0%
AGTTCTATCTAGTAGGTCTA+GGG - chr1_3:28332308-28332327 Msa0112800:CDS 35.0%
! TTCAGTGTTATTGTGAACTC+AGG - chr1_3:28332387-28332406 Msa0112800:CDS 35.0%
!! TTTTCTGAAGATTATGACGC+AGG - chr1_3:28332557-28332576 Msa0112800:CDS 35.0%
!! ATTTTAGTATTCGACCCTAG+AGG - chr1_3:28332584-28332603 Msa0112800:CDS 35.0%
! CACTTTTCTTTCTTAGCAAC+TGG + chr1_3:28332692-28332711 None:intergenic 35.0%
GTAGTTTGATGCAATCTTTG+AGG + chr1_3:28332867-28332886 None:intergenic 35.0%
GAATATCAGGAATATACCTG+AGG + chr1_3:28332935-28332954 None:intergenic 35.0%
ATAAATCACTTCATCCTCCA+AGG - chr1_3:28332971-28332990 Msa0112800:intron 35.0%
TAAATCACTTCATCCTCCAA+GGG - chr1_3:28332972-28332991 Msa0112800:intron 35.0%
CAATGAATTGTTAGCCATGT+AGG + chr1_3:28333070-28333089 None:intergenic 35.0%
!! AATGTTCTGTGTTGGTTTGT+AGG - chr1_3:28333151-28333170 Msa0112800:intron 35.0%
!! ATGTTCTGTGTTGGTTTGTA+GGG - chr1_3:28333152-28333171 Msa0112800:intron 35.0%
AACGGCTAATACATTCTTTC+AGG + chr1_3:28333190-28333209 None:intergenic 35.0%
!! CCTCAATAAGTACTGGAAAA+TGG + chr1_3:28333364-28333383 None:intergenic 35.0%
! CCATTTTCCAGTACTTATTG+AGG - chr1_3:28333361-28333380 Msa0112800:CDS 35.0%
GATACAACCTCAATAAGTAC+TGG + chr1_3:28333371-28333390 None:intergenic 35.0%
! GAGTAATGATGGAAAAAGCT+TGG - chr1_3:28333430-28333449 Msa0112800:CDS 35.0%
CGTAATACTTGCGATCAAAA+TGG - chr1_3:28333526-28333545 Msa0112800:intron 35.0%
AGCATTTGTGATACTGTTTG+AGG - chr1_3:28333576-28333595 Msa0112800:intron 35.0%
GAAGCTTAAGTGATCCAAAT+AGG - chr1_3:28333946-28333965 Msa0112800:intron 35.0%
TTAAGCCAAGCATTCCTATT+TGG + chr1_3:28333963-28333982 None:intergenic 35.0%
AACAGTCACTTCTTTCAGTT+TGG - chr1_3:28334014-28334033 Msa0112800:CDS 35.0%
GATTGTTGCAATTCTTGGAT+TGG - chr1_3:28334279-28334298 Msa0112800:intron 35.0%
AGCAGGAGATACATATATCA+CGG + chr1_3:28334382-28334401 None:intergenic 35.0%
GTATCTCCTGCTTAGATAAA+CGG - chr1_3:28334390-28334409 Msa0112800:intron 35.0%
AGTAAACCGTTTATCTAAGC+AGG + chr1_3:28334399-28334418 None:intergenic 35.0%
AATAATGTAACGGCGTGTTT+GGG + chr1_3:28334568-28334587 None:intergenic 35.0%
AAATAATGTAACGGCGTGTT+TGG + chr1_3:28334569-28334588 None:intergenic 35.0%
TAACTAGATTAAGGCAAAGG+TGG + chr1_3:28334814-28334833 None:intergenic 35.0%
GTCTAACTAGATTAAGGCAA+AGG + chr1_3:28334817-28334836 None:intergenic 35.0%
TATTTGCACGTAAAGTAGAG+CGG + chr1_3:28334966-28334985 None:intergenic 35.0%
CTTGACCATGAATGAATTTC+TGG + chr1_3:28335039-28335058 None:intergenic 35.0%
TAATTGGACACTAATTGCAC+AGG - chr1_3:28335239-28335258 Msa0112800:CDS 35.0%
TCATCCATCTGATCAAATAG+TGG + chr1_3:28335267-28335286 None:intergenic 35.0%
TATTTGATCAGATGGATGAC+AGG - chr1_3:28335268-28335287 Msa0112800:CDS 35.0%
!! TCAGTTGTTTGCTATGTAAC+CGG + chr1_3:28335623-28335642 None:intergenic 35.0%
! CATGTTTTATACAAGCAGCT+TGG - chr1_3:28335688-28335707 Msa0112800:intron 35.0%
TTATACAAGCAGCTTGGTTT+CGG - chr1_3:28335694-28335713 Msa0112800:intron 35.0%
GAGAATGAAAGAGACAAATG+AGG - chr1_3:28335722-28335741 Msa0112800:intron 35.0%
GAAAGAGACAAATGAGGTTA+AGG - chr1_3:28335728-28335747 Msa0112800:intron 35.0%
GACAAATGAGGTTAAGGAAA+AGG - chr1_3:28335734-28335753 Msa0112800:intron 35.0%
ATACCACTAGGCTACTTCTT+CGG + chr1_3:28332270-28332289 None:intergenic 40.0%
TCGAGTGAGTTCTATCTAGT+AGG - chr1_3:28332301-28332320 Msa0112800:CDS 40.0%
GAGTTCTATCTAGTAGGTCT+AGG - chr1_3:28332307-28332326 Msa0112800:CDS 40.0%
AGAAGGTGAAGAAAGTCACA+TGG - chr1_3:28332442-28332461 Msa0112800:CDS 40.0%
AAATGAGTGCATACGAGATG+AGG - chr1_3:28332465-28332484 Msa0112800:CDS 40.0%
ATGAGGAGTGAATACAAGCA+TGG - chr1_3:28332482-28332501 Msa0112800:CDS 40.0%
AAGCCATCATCAAATGGAAG+AGG - chr1_3:28332521-28332540 Msa0112800:CDS 40.0%
AGTCCTCTTCCATTTGATGA+TGG + chr1_3:28332527-28332546 None:intergenic 40.0%
TTATTGCGACATTCTTTGGC+TGG + chr1_3:28333045-28333064 None:intergenic 40.0%
GGCATTATTGCGACATTCTT+TGG + chr1_3:28333049-28333068 None:intergenic 40.0%
ATGTCGCAATAATGCCTACA+TGG - chr1_3:28333053-28333072 Msa0112800:intron 40.0%
TTGTACTCGCTCACACTTTA+CGG + chr1_3:28333121-28333140 None:intergenic 40.0%
GGTCAGAAATGATTGCATCA+AGG - chr1_3:28333319-28333338 Msa0112800:CDS 40.0%
! TCTAAGATAACCAAGGCAAG+TGG - chr1_3:28333408-28333427 Msa0112800:CDS 40.0%
GTGATCCAAATAGGAATGCT+TGG - chr1_3:28333955-28333974 Msa0112800:intron 40.0%
TGATGAATTTGCAGCTCTTC+AGG - chr1_3:28334208-28334227 Msa0112800:CDS 40.0%
CTGCAACATGTGTACTTGTT+AGG + chr1_3:28334243-28334262 None:intergenic 40.0%
GCTGTGATTGTTGCAATTCT+TGG - chr1_3:28334274-28334293 Msa0112800:intron 40.0%
CAGCTTAAGCTATACTAGAG+AGG - chr1_3:28334489-28334508 Msa0112800:intron 40.0%
ACCTTCAATCGACCACTATA+CGG - chr1_3:28334606-28334625 Msa0112800:CDS 40.0%
GAAGGACAGATACAGAAAGA+TGG - chr1_3:28334648-28334667 Msa0112800:CDS 40.0%
! CATTTTCCTTCAGGTAACGA+GGG + chr1_3:28334691-28334710 None:intergenic 40.0%
! ACATTTTCCTTCAGGTAACG+AGG + chr1_3:28334692-28334711 None:intergenic 40.0%
ACGCCATGACCAATTTAGAT+GGG - chr1_3:28334715-28334734 Msa0112800:CDS 40.0%
CTACCCATCTAAATTGGTCA+TGG + chr1_3:28334721-28334740 None:intergenic 40.0%
GAGTAGCTACCCATCTAAAT+TGG + chr1_3:28334727-28334746 None:intergenic 40.0%
ATGTCACGTCTTAGATCAAC+AGG + chr1_3:28334785-28334804 None:intergenic 40.0%
TTTAGATTAGACGGATCTGC+TGG - chr1_3:28334930-28334949 Msa0112800:intron 40.0%
TTAGATTAGACGGATCTGCT+GGG - chr1_3:28334931-28334950 Msa0112800:intron 40.0%
! GACCAATGCGATCTCAATTT+TGG - chr1_3:28335062-28335081 Msa0112800:intron 40.0%
GACCAAAATTGAGATCGCAT+TGG + chr1_3:28335067-28335086 None:intergenic 40.0%
GGTACCACTATTTGATCAGA+TGG - chr1_3:28335260-28335279 Msa0112800:CDS 40.0%
TTAGATTCTTGCACGACCAA+CGG - chr1_3:28335402-28335421 Msa0112800:CDS 40.0%
AAATGCTTCGACTTCTGTGA+TGG + chr1_3:28335556-28335575 None:intergenic 40.0%
GATTCTATTCACATCAGTGG+AGG - chr1_3:28335655-28335674 Msa0112800:CDS 40.0%
CTAGTAGGTAGTTTAAGGAG+AGG - chr1_3:28335870-28335889 Msa0112800:intron 40.0%
GATGCTGAACTTGATATGCT+TGG - chr1_3:28335909-28335928 Msa0112800:intron 40.0%
TACCACTAGGCTACTTCTTC+GGG + chr1_3:28332269-28332288 None:intergenic 45.0%
CAGGAATATACCTGAGGAAG+TGG + chr1_3:28332929-28332948 None:intergenic 45.0%
TGTACTCGCTCACACTTTAC+GGG + chr1_3:28333120-28333139 None:intergenic 45.0%
ATCAAGGCATGTAGTTCGCT+TGG - chr1_3:28333335-28333354 Msa0112800:CDS 45.0%
CATCATTACTCCACTTGCCT+TGG + chr1_3:28333421-28333440 None:intergenic 45.0%
CAAGGCAAGTGGAGTAATGA+TGG - chr1_3:28333419-28333438 Msa0112800:CDS 45.0%
! ATGATGGAAAAAGCTTGGGC+TGG - chr1_3:28333435-28333454 Msa0112800:CDS 45.0%
ACTTATGTGAGCGTACGTAC+TGG - chr1_3:28333549-28333568 Msa0112800:intron 45.0%
ATTGTCTTTGGTGGGGATTG+AGG - chr1_3:28334099-28334118 Msa0112800:CDS 45.0%
!! GTGCTTTTTGCACTGCTCAT+AGG - chr1_3:28334301-28334320 Msa0112800:intron 45.0%
TGGAAGAGTGGCGAATAAGA+AGG - chr1_3:28334630-28334649 Msa0112800:CDS 45.0%
CAGAAAGATGGATGCATCAC+CGG - chr1_3:28334660-28334679 Msa0112800:CDS 45.0%
GACGCCATGACCAATTTAGA+TGG - chr1_3:28334714-28334733 Msa0112800:CDS 45.0%
TTTAGATGGGTAGCTACTCG+TGG - chr1_3:28334728-28334747 Msa0112800:CDS 45.0%
AAACCAACTCTATGCACTCC+AGG - chr1_3:28335318-28335337 Msa0112800:CDS 45.0%
!! TGAAGAGCATTTCGTCGACA+GGG + chr1_3:28335368-28335387 None:intergenic 45.0%
!! ATGAAGAGCATTTCGTCGAC+AGG + chr1_3:28335369-28335388 None:intergenic 45.0%
GAAATGCTCTTCATCGTTCG+AGG - chr1_3:28335375-28335394 Msa0112800:CDS 45.0%
AAAACCAGTTCTACCACCGT+TGG + chr1_3:28335421-28335440 None:intergenic 45.0%
AACACCTGCAGAATAAGCTC+AGG - chr1_3:28335444-28335463 Msa0112800:CDS 45.0%
! CGGTGAAGAGCTTTTACCAT+GGG - chr1_3:28335476-28335495 Msa0112800:CDS 45.0%
ATGAAGGAAACACAGCAGTC+GGG + chr1_3:28335515-28335534 None:intergenic 45.0%
GATGAAGGAAACACAGCAGT+CGG + chr1_3:28335516-28335535 None:intergenic 45.0%
!! GTTGTTTGCTATGTAACCGG+CGG + chr1_3:28335620-28335639 None:intergenic 45.0%
ATTCACATCAGTGGAGGACA+TGG - chr1_3:28335661-28335680 Msa0112800:CDS 45.0%
TTCACATCAGTGGAGGACAT+GGG - chr1_3:28335662-28335681 Msa0112800:CDS 45.0%
ATATTTCTCTGCACCTCTGC+AGG - chr1_3:28335809-28335828 Msa0112800:intron 45.0%
!!! TAATTAAAATATGAATTTTA+TGG - chr1_3:28333217-28333236 Msa0112800:intron 5.0%
ATCCCGAAGAAGTAGCCTAG+TGG - chr1_3:28332264-28332283 Msa0112800:CDS 50.0%
GCTCCTTCATCTCGTGTTTC+TGG - chr1_3:28332818-28332837 Msa0112800:intron 50.0%
CGACCAGAAACACGAGATGA+AGG + chr1_3:28332824-28332843 None:intergenic 50.0%
!! TCATCTCGTGTTTCTGGTCG+AGG - chr1_3:28332824-28332843 Msa0112800:intron 50.0%
ACATGTGATGAAGCCCTTGG+AGG + chr1_3:28332988-28333007 None:intergenic 50.0%
TCCAAGGGCTTCATCACATG+TGG - chr1_3:28332987-28333006 Msa0112800:intron 50.0%
ACCACATGTGATGAAGCCCT+TGG + chr1_3:28332991-28333010 None:intergenic 50.0%
GGTGGGGATTGAGGAATCTA+AGG - chr1_3:28334108-28334127 Msa0112800:CDS 50.0%
GCCGTATAGTGGTCGATTGA+AGG + chr1_3:28334610-28334629 None:intergenic 50.0%
TCAATCGACCACTATACGGC+TGG - chr1_3:28334610-28334629 Msa0112800:CDS 50.0%
!! GGCGTCGAACATTTTCCTTC+AGG + chr1_3:28334700-28334719 None:intergenic 50.0%
GTTCCTGGAGTGCATAGAGT+TGG + chr1_3:28335324-28335343 None:intergenic 50.0%
TCGCGAACTATGCAAGTTCC+TGG + chr1_3:28335339-28335358 None:intergenic 50.0%
GGAACTTGCATAGTTCGCGA+AGG - chr1_3:28335339-28335358 Msa0112800:CDS 50.0%
ATAAGCTCAGGCGACTTCTG+CGG - chr1_3:28335456-28335475 Msa0112800:CDS 50.0%
! GCGGTGAAGAGCTTTTACCA+TGG - chr1_3:28335475-28335494 Msa0112800:CDS 50.0%
!! CTCACTGTGCGAGTTGATGA+AGG + chr1_3:28335531-28335550 None:intergenic 50.0%
AGCATTTGCACTCATTGCCG+AGG - chr1_3:28335569-28335588 Msa0112800:CDS 50.0%
TTGTAGCAGCACAATTCCGC+CGG - chr1_3:28335601-28335620 Msa0112800:CDS 50.0%
TGCAGCACTACCACTTCCTC+AGG - chr1_3:28332916-28332935 Msa0112800:intron 55.0%
CCACTCTTCCAGCCGTATAG+TGG + chr1_3:28334621-28334640 None:intergenic 55.0%
CCACTATACGGCTGGAAGAG+TGG - chr1_3:28334618-28334637 Msa0112800:CDS 55.0%
AGCTACTCGTGGAGTTGACG+AGG - chr1_3:28334739-28334758 Msa0112800:CDS 55.0%
GATTCTTGCACGACCAACGG+TGG - chr1_3:28335405-28335424 Msa0112800:CDS 55.0%
ACGACCAACGGTGGTAGAAC+TGG - chr1_3:28335414-28335433 Msa0112800:CDS 55.0%
GTCGCCTGAGCTTATTCTGC+AGG + chr1_3:28335451-28335470 None:intergenic 55.0%
! GATCCTTTTGGCACCTGCAG+AGG + chr1_3:28335825-28335844 None:intergenic 55.0%
TCAGGTAACGAGGGAGCTGC+CGG + chr1_3:28334682-28334701 None:intergenic 60.0%
GCAGCTCCCTCGTTACCTGA+AGG - chr1_3:28334682-28334701 Msa0112800:CDS 60.0%
GAAACACAGCAGTCGGGCGA+GGG + chr1_3:28335509-28335528 None:intergenic 60.0%
GCACCTCTGCAGGTGCCAAA+AGG - chr1_3:28335819-28335838 Msa0112800:intron 60.0%
AGCAGTCGGGCGAGGGTCTA+AGG + chr1_3:28335502-28335521 None:intergenic 65.0%
GGAAACACAGCAGTCGGGCG+AGG + chr1_3:28335510-28335529 None:intergenic 65.0%
! GGGCGAGGGTCTAAGGCCCA+TGG + chr1_3:28335495-28335514 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 28332108 28335982 28332108 ID=Msa0112800;Name=Msa0112800
chr1_3 mRNA 28332108 28335982 28332108 ID=Msa0112800-mRNA-1;Parent=Msa0112800;Name=Msa0112800-mRNA-1;_AED=0.10;_eAED=0.10;_QI=18|1|1|1|1|1|7|8|708
chr1_3 exon 28332108 28332829 28332108 ID=Msa0112800-mRNA-1:exon:9354;Parent=Msa0112800-mRNA-1
chr1_3 exon 28333252 28333488 28333252 ID=Msa0112800-mRNA-1:exon:9353;Parent=Msa0112800-mRNA-1
chr1_3 exon 28333757 28333868 28333757 ID=Msa0112800-mRNA-1:exon:9352;Parent=Msa0112800-mRNA-1
chr1_3 exon 28333961 28334253 28333961 ID=Msa0112800-mRNA-1:exon:9351;Parent=Msa0112800-mRNA-1
chr1_3 exon 28334593 28334805 28334593 ID=Msa0112800-mRNA-1:exon:9350;Parent=Msa0112800-mRNA-1
chr1_3 exon 28335153 28335681 28335153 ID=Msa0112800-mRNA-1:exon:9349;Parent=Msa0112800-mRNA-1
chr1_3 exon 28335936 28335982 28335936 ID=Msa0112800-mRNA-1:exon:9348;Parent=Msa0112800-mRNA-1
chr1_3 five_prime_UTR 28335965 28335982 28335965 ID=Msa0112800-mRNA-1:five_prime_utr;Parent=Msa0112800-mRNA-1
chr1_3 CDS 28335936 28335964 28335936 ID=Msa0112800-mRNA-1:cds;Parent=Msa0112800-mRNA-1
chr1_3 CDS 28335153 28335681 28335153 ID=Msa0112800-mRNA-1:cds;Parent=Msa0112800-mRNA-1
chr1_3 CDS 28334593 28334805 28334593 ID=Msa0112800-mRNA-1:cds;Parent=Msa0112800-mRNA-1
chr1_3 CDS 28333961 28334253 28333961 ID=Msa0112800-mRNA-1:cds;Parent=Msa0112800-mRNA-1
chr1_3 CDS 28333757 28333868 28333757 ID=Msa0112800-mRNA-1:cds;Parent=Msa0112800-mRNA-1
chr1_3 CDS 28333252 28333488 28333252 ID=Msa0112800-mRNA-1:cds;Parent=Msa0112800-mRNA-1
chr1_3 CDS 28332116 28332829 28332116 ID=Msa0112800-mRNA-1:cds;Parent=Msa0112800-mRNA-1
chr1_3 three_prime_UTR 28332108 28332115 28332108 ID=Msa0112800-mRNA-1:three_prime_utr;Parent=Msa0112800-mRNA-1
Gene Sequence

>Msa0112800

ATGGCTTCTGTTATATCAAGAGTCGTAAGGTTTCATGATGATCTTGAAAAAGAGAAATTACAAGAAGGTGAAGAAAGTCACATGGAAATGAGTGCATACGAGATGAGGAGTGAATACAAGCATGGAAAAGATGCAATAAACAAGCCATCATCAAATGGAAGAGGACTTTCAAGAGTTTTTTCTGAAGATTATGACGCAGGAGAGATTTTAGTATTCGACCCTAGAGGACCTAGAATAAATTTATGGAACAAAATATTCTTAGCAGCTTGTTTGATTTCATTGTTTGTGGATCCACTTTTCTTTTACTTACCAGTTGCTAAGAAAGAAAAGTGTATTGATATGTCAATTGGTCTTGAAGTTTCACTTACTATAATTAGAACATTTGTTGATGCATTTTACATTATTCATATCTATATTAGGTTTCAAACTGCTTATATTGCTCCTTCATCTCGTGTTTCTGGTCGAGGAGAACTCATTATTGATTCCTCAAAGATTGCATCAAACTACATGAAAAAGGAATTTTGGTCTGATCTTGTTGCAGCACTACCACTTCCTCAGGTACTCATTTGGGCTGTAATTCCAAATATAAAAGGGTCAGAAATGATTGCATCAAGGCATGTAGTTCGCTTGGTGTCCATTTTCCAGTACTTATTGAGGTTGTATCTTATTTATCCTCTATCATCTAAGATAACCAAGGCAAGTGGAGTAATGATGGAAAAAGCTTGGGCTGGTGCAGCTTATTATTTAACACTCTATATGCTTGCAAGTCATGTTTTAGGATCAACTTGGTACCTGTTATCAATTGAAAGACAAGATGAATGTTGGAAGAAAGCATGTACACTTCAATATCCACATTGCCAATATCATTATCTTGATTGTCGAAGCTTAAGTGATCCAAATAGGAATGCTTGGCTTAAATCAAGCAATCTATCAGGTCTATGTGATCAGAACAGTCACTTCTTTCAGTTTGGAATTTTTTATGATGCTGTGACTTTGGAAATAACTAGCTCAAACTTCTTAACCAAGTATTATTATTGTCTTTGGTGGGGATTGAGGAATCTAAGCTCTTCAGGAGAGAATCTCCTAACAAGTACACATGTTGCAGAGATAAATTTTGCTGTGATTGTTGCAATTCTTGGATTGGTGCTTTTTGCACTGCTCATAGGAAATATGCAAACGTACCTTCAATCGACCACTATACGGCTGGAAGAGTGGCGAATAAGAAGGACAGATACAGAAAGATGGATGCATCACCGGCAGCTCCCTCGTTACCTGAAGGAAAATGTTCGACGCCATGACCAATTTAGATGGGTAGCTACTCGTGGAGTTGACGAGGAAGCAATTCTTAGAGATCTTCCTGTTGATCTAAGACGTGACATTAAACGCCACCTTTGCCTTAATCTAGTTAGACAAGTACCACTATTTGATCAGATGGATGACAGGATGTTAGATGCAATATGTGAAAGGCTAAAACCAACTCTATGCACTCCAGGAACTTGCATAGTTCGCGAAGGCGACCCTGTCGACGAAATGCTCTTCATCGTTCGAGGACGTTTAGATTCTTGCACGACCAACGGTGGTAGAACTGGTTTTTTCAACACCTGCAGAATAAGCTCAGGCGACTTCTGCGGTGAAGAGCTTTTACCATGGGCCTTAGACCCTCGCCCGACTGCTGTGTTTCCTTCATCAACTCGCACAGTGAGAGCCATCACAGAAGTCGAAGCATTTGCACTCATTGCCGAGGATTTAAAGTTTGTAGCAGCACAATTCCGCCGGTTACATAGCAAACAACTGAGACAAACCTTTAGATTCTATTCACATCAGTGGAGGACATGGGCTGCATGTTTTATACAAGCAGCTTGGTTTCGGTATAAGAGAATGAAAGAGACAAATGAGGTTAAGGAAAAGGAGAATCTTATGATGATGTCAAATGTGAAATATTATGGAAATGATGATTCTCAATATTTCTCTGCACCTCTGCAGGTGCCAAAAGGATCAAGTTATTCAATGTATTCTGGAAAACTAGTAGGTAGTTTAAGGAGAGGAAGAAGCATGAGATATGATGCTGAACTTGATATGCTTGGTACATTAAGGAAGCCTATTGAACCAGATTTTAATGATGACTAG
Protein sequence

>Msa0112800

MASVISRVVRFHDDLEKEKLQEGEESHMEMSAYEMRSEYKHGKDAINKPSSNGRGLSRVFSEDYDAGEILVFDPRGPRINLWNKIFLAACLISLFVDPLFFYLPVAKKEKCIDMSIGLEVSLTIIRTFVDAFYIIHIYIRFQTAYIAPSSRVSGRGELIIDSSKIASNYMKKEFWSDLVAALPLPQVLIWAVIPNIKGSEMIASRHVVRLVSIFQYLLRLYLIYPLSSKITKASGVMMEKAWAGAAYYLTLYMLASHVLGSTWYLLSIERQDECWKKACTLQYPHCQYHYLDCRSLSDPNRNAWLKSSNLSGLCDQNSHFFQFGIFYDAVTLEITSSNFLTKYYYCLWWGLRNLSSSGENLLTSTHVAEINFAVIVAILGLVLFALLIGNMQTYLQSTTIRLEEWRIRRTDTERWMHHRQLPRYLKENVRRHDQFRWVATRGVDEEAILRDLPVDLRRDIKRHLCLNLVRQVPLFDQMDDRMLDAICERLKPTLCTPGTCIVREGDPVDEMLFIVRGRLDSCTTNGGRTGFFNTCRISSGDFCGEELLPWALDPRPTAVFPSSTRTVRAITEVEAFALIAEDLKFVAAQFRRLHSKQLRQTFRFYSHQWRTWAACFIQAAWFRYKRMKETNEVKEKENLMMMSNVKYYGNDDSQYFSAPLQVPKGSSYSMYSGKLVGSLRRGRSMRYDAELDMLGTLRKPIEPDFNDD*