AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0439000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0439000 MtrunA17_Chr6g0467251 86.139 202 9 1 24 206 147 348 1.69e-114 330
Msa0439000 MtrunA17_Chr6g0467121 50.761 197 77 2 26 202 184 380 7.32e-59 189
Msa0439000 MtrunA17_Chr6g0467161 47.368 190 79 2 20 188 149 338 2.80e-52 171
Msa0439000 MtrunA17_Chr2g0292241 40.415 193 86 5 19 182 134 326 2.68e-38 135
Msa0439000 MtrunA17_Chr2g0292211 39.896 193 87 5 19 182 145 337 2.04e-36 130
Msa0439000 MtrunA17_Chr5g0423911 81.944 72 13 0 119 190 4 75 5.71e-35 119
Msa0439000 MtrunA17_Chr2g0291751 37.931 203 97 5 19 192 134 336 8.00e-35 126
Msa0439000 MtrunA17_Chr2g0292151 36.916 214 104 6 19 201 134 347 1.16e-34 125
Msa0439000 MtrunA17_Chr1g0188431 39.080 174 89 2 26 182 150 323 2.36e-34 124
Msa0439000 MtrunA17_Chr2g0292111 36.946 203 99 5 19 192 134 336 1.91e-33 122
Msa0439000 MtrunA17_Chr2g0292251 35.681 213 103 7 21 199 135 347 2.23e-33 122
Msa0439000 MtrunA17_Chr2g0292281 37.705 183 95 6 19 182 30 212 1.89e-32 117
Msa0439000 MtrunA17_Chr2g0292101 35.821 201 100 4 22 193 133 333 1.56e-31 117
Msa0439000 MtrunA17_Chr2g0292181 35.751 193 95 5 19 182 143 335 2.62e-31 117
Msa0439000 MtrunA17_Chr2g0292091 37.895 190 89 5 22 182 132 321 4.40e-31 116
Msa0439000 MtrunA17_Chr2g0292291 38.660 194 89 5 19 182 135 328 7.92e-31 115
Msa0439000 MtrunA17_Chr2g0292261 35.377 212 108 5 19 201 134 345 7.87e-30 112
Msa0439000 MtrunA17_Chr2g0292271 36.946 203 97 6 26 198 141 342 1.04e-29 112
Msa0439000 MtrunA17_Chr7g0254231 37.222 180 93 2 22 182 129 307 1.74e-29 111
Msa0439000 MtrunA17_Chr6g0477071 36.782 174 93 2 26 182 165 338 7.35e-29 110
Msa0439000 MtrunA17_Chr4g0013191 40.000 185 85 6 22 182 154 336 9.58e-29 109
Msa0439000 MtrunA17_Chr4g0013201 38.764 178 87 4 26 182 158 334 5.24e-28 107
Msa0439000 MtrunA17_Chr7g0225221 34.973 183 96 3 23 182 134 316 1.15e-27 106
Msa0439000 MtrunA17_Chr4g0072091 37.234 188 91 4 26 186 136 323 3.27e-27 105
Msa0439000 MtrunA17_Chr5g0405991 34.426 183 90 5 26 182 134 312 4.56e-27 105
Msa0439000 MtrunA17_Chr8g0364291 33.684 190 98 5 26 187 136 325 8.25e-27 104
Msa0439000 MtrunA17_Chr4g0013721 33.333 189 98 5 26 188 212 398 1.41e-26 105
Msa0439000 MtrunA17_Chr1g0197411 36.216 185 90 4 26 182 142 326 2.87e-26 103
Msa0439000 MtrunA17_Chr1g0175561 36.170 188 87 6 26 182 137 322 3.13e-26 102
Msa0439000 MtrunA17_Chr1g0164891 33.880 183 93 3 28 182 206 388 3.55e-26 103
Msa0439000 MtrunA17_Chr5g0402581 32.812 192 100 3 20 182 131 322 4.84e-26 102
Msa0439000 MtrunA17_Chr2g0320071 34.807 181 96 3 23 182 134 313 6.57e-26 102
Msa0439000 MtrunA17_Chr3g0129061 32.620 187 102 3 23 186 137 322 6.81e-26 102
Msa0439000 MtrunA17_Chr4g0075241 33.333 183 96 3 26 182 141 323 7.66e-26 102
Msa0439000 MtrunA17_Chr4g0046131 34.409 186 93 4 26 182 157 342 8.36e-26 102
Msa0439000 MtrunA17_Chr4g0013821 33.333 195 102 5 26 194 140 332 8.53e-26 102
Msa0439000 MtrunA17_Chr3g0106901 35.393 178 93 4 26 182 143 319 1.02e-25 101
Msa0439000 MtrunA17_Chr8g0371381 34.031 191 94 6 20 182 129 315 1.08e-25 101
Msa0439000 MtrunA17_Chr3g0114461 34.225 187 100 5 22 186 7 192 1.25e-25 98.2
Msa0439000 MtrunA17_Chr5g0404721 31.959 194 104 4 22 187 138 331 2.46e-25 100
Msa0439000 MtrunA17_Chr7g0244461 34.615 182 97 3 22 182 128 308 2.62e-25 100
Msa0439000 MtrunA17_Chr1g0148021 32.609 184 97 4 26 182 141 324 3.67e-25 100
Msa0439000 MtrunA17_Chr3g0129051 34.225 187 99 3 23 186 137 322 3.96e-25 99.8
Msa0439000 MtrunA17_Chr4g0013171 32.620 187 103 3 22 186 136 321 4.11e-25 99.8
Msa0439000 MtrunA17_Chr7g0268501 31.551 187 100 5 27 186 135 320 7.37e-25 99.0
Msa0439000 MtrunA17_Chr7g0268521 31.551 187 100 5 27 186 135 320 7.37e-25 99.0
Msa0439000 MtrunA17_Chr3g0128061 33.514 185 95 4 26 182 138 322 1.07e-24 99.0
Msa0439000 MtrunA17_Chr3g0114451 34.270 178 95 4 26 182 138 314 1.16e-24 98.6
Msa0439000 MtrunA17_Chr5g0438021 32.620 187 102 3 23 186 140 325 1.50e-24 98.2
Msa0439000 MtrunA17_Chr5g0400331 32.461 191 94 5 26 182 138 327 2.21e-24 98.2
Msa0439000 MtrunA17_Chr2g0320101 34.254 181 97 4 23 182 134 313 2.34e-24 97.8
Msa0439000 MtrunA17_Chr7g0224371 32.597 181 101 5 22 182 139 318 4.99e-24 97.1
Msa0439000 MtrunA17_Chr5g0402571 31.633 196 105 3 20 186 131 326 6.79e-24 96.7
Msa0439000 MtrunA17_Chr5g0432441 32.796 186 99 3 26 186 142 326 1.88e-23 95.5
Msa0439000 MtrunA17_Chr1g0213061 33.889 180 95 3 26 182 139 317 5.58e-23 94.4
Msa0439000 MtrunA17_Chr4g0053871 34.078 179 96 4 26 182 142 320 5.58e-23 94.0
Msa0439000 MtrunA17_Chr2g0299691 32.804 189 95 4 26 182 166 354 6.95e-23 94.4
Msa0439000 MtrunA17_Chr2g0320091 32.787 183 100 4 22 182 133 314 8.63e-23 93.6
Msa0439000 MtrunA17_Chr4g0024121 30.811 185 100 5 26 182 138 322 1.02e-22 93.6
Msa0439000 MtrunA17_Chr1g0194151 33.511 188 96 5 28 186 146 333 1.08e-22 93.6
Msa0439000 MtrunA17_Chr2g0329641 32.984 191 96 4 27 186 146 335 1.21e-22 93.6
Msa0439000 MtrunA17_Chr8g0372601 29.101 189 106 4 22 182 138 326 1.44e-22 93.2
Msa0439000 MtrunA17_Chr2g0322861 32.984 191 99 6 26 187 197 387 1.44e-22 94.0
Msa0439000 MtrunA17_Chr3g0106921 33.514 185 98 4 22 182 133 316 1.91e-22 92.8
Msa0439000 MtrunA17_Chr4g0013181 31.551 187 105 4 22 186 168 353 2.23e-22 92.8
Msa0439000 MtrunA17_Chr1g0155611 31.579 190 101 5 22 182 154 343 5.04e-22 92.0
Msa0439000 MtrunA17_Chr4g0068621 30.366 191 107 4 22 186 146 336 5.08e-22 91.7
Msa0439000 MtrunA17_Chr1g0157051 31.148 183 97 4 26 182 135 314 9.12e-22 90.9
Msa0439000 MtrunA17_Chr6g0460711 33.871 186 95 4 26 186 132 314 1.35e-21 90.5
Msa0439000 MtrunA17_Chr6g0451571 33.690 187 98 5 22 182 144 330 1.58e-21 90.5
Msa0439000 MtrunA17_Chr7g0244451 33.333 186 102 3 22 186 132 316 3.95e-21 89.0
Msa0439000 MtrunA17_Chr2g0279041 31.795 195 103 4 22 186 131 325 4.10e-21 89.0
Msa0439000 MtrunA17_Chr4g0038161 33.514 185 99 3 26 186 136 320 4.64e-21 89.0
Msa0439000 MtrunA17_Chr2g0320061 32.967 182 99 5 23 182 134 314 5.39e-21 88.6
Msa0439000 MtrunA17_Chr7g0254201 33.690 187 97 5 26 188 131 314 5.41e-21 88.6
Msa0439000 MtrunA17_Chr7g0258811 33.511 188 92 7 26 182 182 367 6.99e-21 89.0
Msa0439000 MtrunA17_Chr5g0401431 32.609 184 97 4 26 182 142 325 7.00e-21 88.6
Msa0439000 MtrunA17_Chr3g0136751 33.679 193 99 4 23 186 130 322 8.70e-21 88.2
Msa0439000 MtrunA17_Chr4g0038151 32.432 185 101 3 26 186 137 321 1.76e-20 87.4
Msa0439000 MtrunA17_Chr4g0063671 32.275 189 97 4 25 182 128 316 3.44e-20 86.7
Msa0439000 MtrunA17_Chr3g0129041 31.551 187 104 3 23 186 137 322 3.63e-20 86.7
Msa0439000 MtrunA17_Chr3g0107881 34.054 185 96 6 23 182 137 320 7.03e-20 85.9
Msa0439000 MtrunA17_Chr1g0194271 32.308 195 96 4 28 186 146 340 8.29e-20 85.9
Msa0439000 MtrunA17_Chr2g0320051 33.516 182 98 5 23 182 134 314 1.27e-19 85.1
Msa0439000 MtrunA17_Chr4g0070951 30.457 197 106 4 22 187 134 330 1.49e-19 84.7
Msa0439000 MtrunA17_Chr1g0204011 29.508 183 101 3 26 182 138 318 2.25e-19 84.3
Msa0439000 MtrunA17_Chr5g0432351 32.955 176 95 5 29 182 143 317 2.46e-19 84.3
Msa0439000 MtrunA17_Chr4g0076211 32.275 189 98 6 21 182 144 329 2.59e-19 84.3
Msa0439000 MtrunA17_Chr2g0279061 30.769 195 105 4 22 186 72 266 5.00e-19 82.4
Msa0439000 MtrunA17_Chr5g0432341 32.955 176 95 5 29 182 143 317 6.56e-19 83.2
Msa0439000 MtrunA17_Chr4g0043391 33.862 189 94 6 26 186 139 324 3.70e-18 80.9
Msa0439000 MtrunA17_Chr1g0181511 28.108 185 108 3 23 182 135 319 3.89e-18 80.9
Msa0439000 MtrunA17_Chr4g0074851 29.255 188 108 3 26 188 143 330 4.13e-18 80.9
Msa0439000 MtrunA17_Chr1g0181431 29.121 182 104 3 26 182 138 319 4.25e-18 80.9
Msa0439000 MtrunA17_Chr4g0038171 33.862 189 94 6 26 186 136 321 4.30e-18 80.9
Msa0439000 MtrunA17_Chr2g0296621 31.492 181 99 7 26 182 158 337 4.93e-18 80.9
Msa0439000 MtrunA17_Chr2g0320031 30.769 182 103 5 23 182 135 315 5.94e-18 80.5
Msa0439000 MtrunA17_Chr5g0413051 30.978 184 96 4 34 186 194 377 6.09e-17 78.2
Msa0439000 MtrunA17_Chr1g0181481 27.568 185 109 3 23 182 135 319 1.09e-16 77.0
Msa0439000 MtrunA17_Chr5g0432331 30.114 176 100 5 29 182 174 348 1.34e-15 73.9
Msa0439000 MtrunA17_Chr1g0181501 28.729 181 103 4 27 182 6 185 1.34e-15 72.0
Msa0439000 MtrunA17_Chr2g0278671 29.744 195 107 4 22 186 129 323 1.99e-15 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0439000 AT1G71695.1 39.614 207 101 4 22 206 153 357 6.14e-41 142
Msa0439000 AT4G33420.1 36.559 186 99 3 20 186 140 325 1.34e-31 117
Msa0439000 AT4G33420.2 37.634 186 97 4 20 186 187 372 2.57e-31 117
Msa0439000 AT4G08770.1 34.722 216 109 6 22 206 132 346 1.88e-30 114
Msa0439000 AT4G37520.1 35.106 188 95 4 26 186 142 329 3.08e-29 111
Msa0439000 AT4G37520.2 35.106 188 95 4 26 186 139 326 3.15e-29 111
Msa0439000 AT5G19880.1 37.968 187 86 5 26 182 139 325 9.40e-29 110
Msa0439000 AT2G18980.1 36.170 188 93 5 22 182 132 319 3.17e-28 108
Msa0439000 AT2G22420.1 34.392 189 96 4 26 188 135 321 1.91e-27 106
Msa0439000 AT4G08780.1 32.870 216 113 6 22 206 132 346 4.20e-27 105
Msa0439000 AT3G01190.1 31.937 191 103 4 22 186 132 321 4.46e-27 105
Msa0439000 AT5G14130.1 34.031 191 100 4 22 186 140 330 5.31e-27 105
Msa0439000 AT5G14130.2 34.031 191 100 4 22 186 181 371 6.56e-27 105
Msa0439000 AT2G18150.1 31.443 194 105 5 22 187 144 337 7.86e-27 105
Msa0439000 AT4G37530.1 34.043 188 97 4 26 186 142 329 2.01e-26 103
Msa0439000 AT5G15180.1 32.447 188 100 4 22 182 138 325 2.14e-26 103
Msa0439000 AT5G40150.1 34.783 184 97 3 26 186 145 328 2.74e-26 103
Msa0439000 AT4G36430.1 32.632 190 100 5 21 182 137 326 3.71e-26 103
Msa0439000 AT3G28200.1 34.759 187 100 3 22 186 130 316 4.84e-26 102
Msa0439000 AT5G06730.1 34.694 196 99 5 26 192 145 340 4.95e-26 103
Msa0439000 AT4G30170.1 33.511 188 98 4 22 182 134 321 1.11e-25 101
Msa0439000 AT3G32980.1 32.212 208 105 6 26 202 143 345 2.68e-25 101
Msa0439000 AT2G38390.1 35.025 197 98 6 22 188 139 335 2.91e-25 100
Msa0439000 AT2G38380.1 36.788 193 92 7 26 188 143 335 6.09e-25 100
Msa0439000 AT5G17820.1 34.637 179 94 4 26 182 132 309 1.67e-24 98.6
Msa0439000 AT3G50990.1 31.746 189 101 6 22 182 149 337 2.06e-24 98.6
Msa0439000 AT3G03670.1 34.694 196 99 5 20 186 126 321 2.34e-24 98.2
Msa0439000 AT3G17070.2 33.871 186 94 5 26 182 87 272 2.76e-24 97.1
Msa0439000 AT2G18140.1 30.769 195 107 5 21 187 142 336 2.92e-24 98.2
Msa0439000 AT5G17820.2 34.637 179 94 4 26 182 185 362 3.37e-24 98.6
Msa0439000 AT1G05240.1 34.715 193 97 5 23 186 133 325 3.66e-24 97.8
Msa0439000 AT1G05250.1 34.715 193 97 5 23 186 133 325 3.66e-24 97.8
Msa0439000 AT5G66390.1 31.217 189 102 4 22 182 141 329 3.96e-24 97.8
Msa0439000 AT3G17070.1 33.871 186 94 5 26 182 150 335 7.79e-24 97.1
Msa0439000 AT5G58400.1 32.275 189 103 3 22 186 138 325 8.88e-24 96.7
Msa0439000 AT5G42180.1 32.240 183 95 4 26 182 134 313 9.54e-24 96.3
Msa0439000 AT5G19890.1 32.143 196 101 4 22 187 135 328 1.08e-23 96.7
Msa0439000 AT5G67400.1 33.862 189 98 4 25 186 141 329 1.25e-23 96.3
Msa0439000 AT4G11290.1 31.937 191 99 7 22 182 133 322 1.49e-23 96.3
Msa0439000 AT1G44970.1 32.474 194 102 5 22 186 153 346 2.46e-23 95.9
Msa0439000 AT1G14540.1 32.787 183 101 3 26 186 133 315 4.31e-23 94.7
Msa0439000 AT5G64120.1 32.984 191 101 5 22 186 139 328 5.75e-23 94.4
Msa0439000 AT5G06720.1 33.333 186 95 5 26 182 144 329 5.80e-23 94.7
Msa0439000 AT5G58390.1 31.746 189 104 4 22 186 129 316 9.06e-23 94.0
Msa0439000 AT1G24110.1 32.461 191 102 6 26 189 135 325 1.50e-22 93.2
Msa0439000 AT3G49110.1 31.250 208 107 6 26 202 145 347 1.99e-22 93.6
Msa0439000 AT3G49120.1 31.250 208 107 6 26 202 144 346 2.15e-22 93.2
Msa0439000 AT3G49960.1 34.043 188 97 5 26 186 142 329 3.46e-22 92.4
Msa0439000 AT4G26010.1 34.254 181 97 6 26 187 133 310 6.31e-22 91.3
Msa0439000 AT4G26010.2 34.254 181 97 6 26 187 161 338 7.47e-22 91.7
Msa0439000 AT2G24800.1 27.753 227 122 5 1 186 104 329 1.13e-21 90.9
Msa0439000 AT1G49570.1 32.308 195 102 4 22 186 156 350 1.75e-21 90.9
Msa0439000 AT5G05340.1 30.851 188 106 3 22 186 138 324 2.40e-21 90.1
Msa0439000 AT4G17690.1 32.778 180 98 4 26 182 139 318 1.22e-20 88.2
Msa0439000 AT1G14550.1 32.597 181 97 5 26 182 138 317 1.59e-20 87.8
Msa0439000 AT1G05260.1 28.796 191 107 6 26 187 136 326 2.21e-20 87.4
Msa0439000 AT2G41480.1 32.804 189 96 4 25 182 136 324 2.56e-20 87.4
Msa0439000 AT2G35380.2 32.124 193 92 6 26 182 54 243 3.02e-20 85.9
Msa0439000 AT4G16270.1 31.746 189 99 6 22 182 158 344 3.97e-20 87.0
Msa0439000 AT5G24070.1 30.890 191 100 4 27 186 146 335 4.81e-20 86.7
Msa0439000 AT2G39040.1 32.461 191 96 8 24 182 157 346 5.04e-20 86.7
Msa0439000 AT2G35380.1 32.124 193 92 6 26 182 142 331 1.18e-19 85.5
Msa0439000 AT5G51890.1 32.044 181 94 4 26 179 137 315 1.47e-19 85.1
Msa0439000 AT1G30870.1 32.984 191 98 6 26 188 159 347 2.32e-19 85.1
Msa0439000 AT5G47000.1 32.258 186 102 5 26 187 146 331 2.84e-19 84.7
Msa0439000 AT5G39580.1 33.684 190 94 6 26 186 133 319 5.46e-19 83.6
Msa0439000 AT3G21770.1 28.495 186 104 4 26 182 139 324 6.69e-19 83.6
Msa0439000 AT1G34510.1 32.967 182 98 6 26 187 133 310 2.42e-18 81.6
Msa0439000 AT4G31760.1 28.947 190 106 5 26 186 137 326 3.70e-18 81.3
Msa0439000 AT2G34060.1 30.108 186 101 5 26 182 157 342 2.49e-17 79.3
Msa0439000 AT1G77100.1 31.016 187 104 4 25 186 133 319 6.72e-17 77.8
Msa0439000 AT2G37130.2 26.738 187 111 4 22 182 106 292 1.51e-16 76.6
Msa0439000 AT2G37130.1 26.738 187 111 4 22 182 137 323 1.99e-16 76.6
Msa0439000 AT5G64100.1 29.534 193 106 5 22 186 141 331 2.26e-16 76.6
Msa0439000 AT4G21960.1 28.571 182 105 3 26 182 142 323 2.74e-16 76.3
Msa0439000 AT1G68850.1 29.231 195 105 6 21 182 137 331 3.01e-16 76.3
Msa0439000 AT4G25980.1 29.534 193 103 5 26 186 135 326 1.48e-15 74.3
Msa0439000 AT4G37530.2 34.559 136 62 4 26 134 142 277 5.75e-15 72.0
Msa0439000 AT5G64110.1 30.526 190 102 5 26 186 142 330 9.49e-15 72.0
Msa0439000 AT5G22410.1 28.191 188 104 6 29 187 141 326 2.38e-14 70.9
Msa0439000 AT2G43480.1 27.128 188 105 5 26 182 145 331 6.16e-14 69.7

Find 42 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTAGATTTCCTATTATT+TGG 0.128939 3_3:-4437325 None:intergenic
TCGAGTTGTTTCTTGAAATT+TGG 0.165142 3_3:-4437022 None:intergenic
GTCCCTCAACGCCGTTAATT+TGG 0.208641 3_3:+4437066 Msa0439000:CDS
CTATCTGGTGCCCACACTTT+TGG 0.251851 3_3:+4436941 Msa0439000:CDS
CCCAATGGCACGTCAAAATC+TGG 0.255614 3_3:-4436794 None:intergenic
AATATTATGTTACGCAATAA+AGG 0.298757 3_3:-4436764 None:intergenic
CTGCATTTACACTGAAGTTT+AGG 0.301903 3_3:-4436829 None:intergenic
GAAATCTAAGGTGGCATCTA+TGG 0.324726 3_3:+4437336 Msa0439000:CDS
TTCACAATCTCCTTAGTCTT+AGG 0.327909 3_3:-4437184 None:intergenic
TCCAAATAATAGGAAATCTA+AGG 0.367020 3_3:+4437324 Msa0439000:CDS
ATGATTCGGTTGAACATTGT+TGG 0.380602 3_3:-4436977 None:intergenic
ACCGATGTAGTTGCTCTATC+TGG 0.387253 3_3:+4436926 Msa0439000:CDS
TCTGTTCGTGCAAATGGTAC+TGG 0.412087 3_3:-4436863 None:intergenic
AGTTCATCTGTTCGTGCAAA+TGG 0.412175 3_3:-4436869 None:intergenic
TTGAAATTTGGTTCGATTGG+TGG 0.414542 3_3:-4437010 None:intergenic
TTGCGTAACATAATATTTGA+AGG 0.420033 3_3:+4436770 Msa0439000:CDS
TGTTCAAACATCCAAATTAA+CGG 0.445931 3_3:-4437077 None:intergenic
ATCCAAATTAACGGCGTTGA+GGG 0.449517 3_3:-4437068 None:intergenic
GTGGGTCGGATTCAATGATT+CGG 0.450356 3_3:-4436991 None:intergenic
TTCTTGAAATTTGGTTCGAT+TGG 0.452274 3_3:-4437013 None:intergenic
TGAAATTTGGTTCGATTGGT+GGG 0.456433 3_3:-4437009 None:intergenic
GGCAAGTCATCCTAAGACTA+AGG 0.457751 3_3:+4437174 Msa0439000:CDS
ATTTGGTTCGATTGGTGGGT+CGG 0.494826 3_3:-4437005 None:intergenic
CCAATGGCACGTCAAAATCT+GGG 0.508698 3_3:-4436793 None:intergenic
CTGTTCGTGCAAATGGTACT+GGG 0.511334 3_3:-4436862 None:intergenic
GCGTTGAGGGACTCCTCATT+CGG 0.514253 3_3:-4437055 None:intergenic
ATCTATGGTGGAAGAGGTGG+TGG 0.516164 3_3:+4437351 Msa0439000:CDS
GGTGGCATCTATGGTGGAAG+AGG 0.527491 3_3:+4437345 Msa0439000:CDS
CTAAACTTCAGTGTAAATGC+AGG 0.534350 3_3:+4436830 Msa0439000:CDS
GGCATCTATGGTGGAAGAGG+TGG 0.536043 3_3:+4437348 Msa0439000:CDS
CTTTCTGGTTAGAAGCAAAG+AGG 0.545522 3_3:-4437207 None:intergenic
GTTTACATCGGATCAAGACT+TGG 0.546163 3_3:+4437153 Msa0439000:CDS
CGAAGCAACATGTCCGAATG+AGG 0.556767 3_3:+4437042 Msa0439000:CDS
CATCCAAATTAACGGCGTTG+AGG 0.565870 3_3:-4437069 None:intergenic
ATCTAAGGTGGCATCTATGG+TGG 0.582875 3_3:+4437339 Msa0439000:CDS
AATGAGCTTGACCAAAAGTG+TGG 0.611586 3_3:-4436952 None:intergenic
AAATAATAGGAAATCTAAGG+TGG 0.648910 3_3:+4437327 Msa0439000:CDS
ATGAGCTTGACCAAAAGTGT+GGG 0.655019 3_3:-4436951 None:intergenic
TCACCAGGGTGTGTTTACAT+CGG 0.667869 3_3:+4437141 Msa0439000:intron
ACCAGATAGAGCAACTACAT+CGG 0.673269 3_3:-4436927 None:intergenic
TACGCAATAAAGGACATCGA+GGG 0.715776 3_3:-4436754 None:intergenic
TTACGCAATAAAGGACATCG+AGG 0.746500 3_3:-4436755 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTATGTTACGCAATAA+AGG - chr3_3:4436767-4436786 None:intergenic 20.0%
!!! ATTTGTTAAAAAACTCTTTC+TGG - chr3_3:4437225-4437244 None:intergenic 20.0%
! TTGCGTAACATAATATTTGA+AGG + chr3_3:4436770-4436789 Msa0439000:CDS 25.0%
!! GATGAACTATTAACTGTTTT+CGG + chr3_3:4436884-4436903 Msa0439000:CDS 25.0%
! TGTTCAAACATCCAAATTAA+CGG - chr3_3:4437080-4437099 None:intergenic 25.0%
! TACATATTATCGAATGTGTT+TGG - chr3_3:4437103-4437122 None:intergenic 25.0%
!!! ATTTGCTAATGCTTTTGTTA+AGG + chr3_3:4437243-4437262 Msa0439000:CDS 25.0%
!!! TTTTGTTAAGGTTAGTCAAT+TGG + chr3_3:4437255-4437274 Msa0439000:CDS 25.0%
! TCCAAATAATAGGAAATCTA+AGG + chr3_3:4437324-4437343 Msa0439000:CDS 25.0%
! ACCTTAGATTTCCTATTATT+TGG - chr3_3:4437328-4437347 None:intergenic 25.0%
! AAATAATAGGAAATCTAAGG+TGG + chr3_3:4437327-4437346 Msa0439000:CDS 25.0%
! TTCTTGAAATTTGGTTCGAT+TGG - chr3_3:4437016-4437035 None:intergenic 30.0%
!! TCGAGTTGTTTCTTGAAATT+TGG - chr3_3:4437025-4437044 None:intergenic 30.0%
! CATGTTTTGCTCCAAATAAT+AGG + chr3_3:4437314-4437333 Msa0439000:CDS 30.0%
CTGCATTTACACTGAAGTTT+AGG - chr3_3:4436832-4436851 None:intergenic 35.0%
CTAAACTTCAGTGTAAATGC+AGG + chr3_3:4436830-4436849 Msa0439000:CDS 35.0%
!! ATGATTCGGTTGAACATTGT+TGG - chr3_3:4436980-4436999 None:intergenic 35.0%
! TGAAATTTGGTTCGATTGGT+GGG - chr3_3:4437012-4437031 None:intergenic 35.0%
! TTGAAATTTGGTTCGATTGG+TGG - chr3_3:4437013-4437032 None:intergenic 35.0%
ATTAACCTCTTGAATCACCA+GGG + chr3_3:4437127-4437146 Msa0439000:intron 35.0%
! TTCACAATCTCCTTAGTCTT+AGG - chr3_3:4437187-4437206 None:intergenic 35.0%
!!! AGTCAATTGGATGTTTTGAC+TGG + chr3_3:4437268-4437287 Msa0439000:CDS 35.0%
!!! GATGTTTTGACTGGAAATCA+AGG + chr3_3:4437277-4437296 Msa0439000:CDS 35.0%
TACGCAATAAAGGACATCGA+GGG - chr3_3:4436757-4436776 None:intergenic 40.0%
TTACGCAATAAAGGACATCG+AGG - chr3_3:4436758-4436777 None:intergenic 40.0%
AGTTCATCTGTTCGTGCAAA+TGG - chr3_3:4436872-4436891 None:intergenic 40.0%
ACCAGATAGAGCAACTACAT+CGG - chr3_3:4436930-4436949 None:intergenic 40.0%
! ATGAGCTTGACCAAAAGTGT+GGG - chr3_3:4436954-4436973 None:intergenic 40.0%
AATGAGCTTGACCAAAAGTG+TGG - chr3_3:4436955-4436974 None:intergenic 40.0%
! ATCCAAATTAACGGCGTTGA+GGG - chr3_3:4437071-4437090 None:intergenic 40.0%
CATTAACCTCTTGAATCACC+AGG + chr3_3:4437126-4437145 Msa0439000:intron 40.0%
GTTTACATCGGATCAAGACT+TGG + chr3_3:4437153-4437172 Msa0439000:CDS 40.0%
!! CTTTCTGGTTAGAAGCAAAG+AGG - chr3_3:4437210-4437229 None:intergenic 40.0%
! GAAATCTAAGGTGGCATCTA+TGG + chr3_3:4437336-4437355 Msa0439000:CDS 40.0%
CCAATGGCACGTCAAAATCT+GGG - chr3_3:4436796-4436815 None:intergenic 45.0%
!! CCAGATTTTGACGTGCCATT+GGG + chr3_3:4436794-4436813 Msa0439000:CDS 45.0%
! AGGCTGTCTTTTCTTCCCAA+TGG - chr3_3:4436812-4436831 None:intergenic 45.0%
CTGTTCGTGCAAATGGTACT+GGG - chr3_3:4436865-4436884 None:intergenic 45.0%
TCTGTTCGTGCAAATGGTAC+TGG - chr3_3:4436866-4436885 None:intergenic 45.0%
ACCGATGTAGTTGCTCTATC+TGG + chr3_3:4436926-4436945 Msa0439000:CDS 45.0%
GTGGGTCGGATTCAATGATT+CGG - chr3_3:4436994-4437013 None:intergenic 45.0%
! ATTTGGTTCGATTGGTGGGT+CGG - chr3_3:4437008-4437027 None:intergenic 45.0%
CATCCAAATTAACGGCGTTG+AGG - chr3_3:4437072-4437091 None:intergenic 45.0%
TCACCAGGGTGTGTTTACAT+CGG + chr3_3:4437141-4437160 Msa0439000:intron 45.0%
GGCAAGTCATCCTAAGACTA+AGG + chr3_3:4437174-4437193 Msa0439000:CDS 45.0%
! ATCTAAGGTGGCATCTATGG+TGG + chr3_3:4437339-4437358 Msa0439000:CDS 45.0%
!! CCCAGATTTTGACGTGCCAT+TGG + chr3_3:4436793-4436812 Msa0439000:CDS 50.0%
CCCAATGGCACGTCAAAATC+TGG - chr3_3:4436797-4436816 None:intergenic 50.0%
! CTATCTGGTGCCCACACTTT+TGG + chr3_3:4436941-4436960 Msa0439000:CDS 50.0%
CGAAGCAACATGTCCGAATG+AGG + chr3_3:4437042-4437061 Msa0439000:CDS 50.0%
GTCCCTCAACGCCGTTAATT+TGG + chr3_3:4437066-4437085 Msa0439000:CDS 50.0%
ACACACCCTGGTGATTCAAG+AGG - chr3_3:4437135-4437154 None:intergenic 50.0%
GATCCGATGTAAACACACCC+TGG - chr3_3:4437147-4437166 None:intergenic 50.0%
! ATCTATGGTGGAAGAGGTGG+TGG + chr3_3:4437351-4437370 Msa0439000:CDS 50.0%
GCGTTGAGGGACTCCTCATT+CGG - chr3_3:4437058-4437077 None:intergenic 55.0%
! GGTGGCATCTATGGTGGAAG+AGG + chr3_3:4437345-4437364 Msa0439000:CDS 55.0%
! GGCATCTATGGTGGAAGAGG+TGG + chr3_3:4437348-4437367 Msa0439000:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 4436716 4437393 4436716 ID=Msa0439000;Name=Msa0439000
chr3_3 mRNA 4436716 4437393 4436716 ID=Msa0439000-mRNA-1;Parent=Msa0439000;Name=Msa0439000-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|1|1|2|0|206
chr3_3 exon 4436716 4437090 4436716 ID=Msa0439000-mRNA-1:exon:951;Parent=Msa0439000-mRNA-1
chr3_3 exon 4437148 4437393 4437148 ID=Msa0439000-mRNA-1:exon:952;Parent=Msa0439000-mRNA-1
chr3_3 CDS 4436716 4437090 4436716 ID=Msa0439000-mRNA-1:cds;Parent=Msa0439000-mRNA-1
chr3_3 CDS 4437148 4437393 4437148 ID=Msa0439000-mRNA-1:cds;Parent=Msa0439000-mRNA-1
Gene Sequence

>Msa0439000

ATGGTTAAAAGTTTGAGAAATATTAATGTTGAATTTGTCCCTCGATGTCCTTTATTGCGTAACATAATATTTGAAGGCCCAGATTTTGACGTGCCATTGGGAAGAAAAGACAGCCTAAACTTCAGTGTAAATGCAGGAGATAATCTCCCAGTACCATTTGCACGAACAGATGAACTATTAACTGTTTTCGGATCAAAAAAATTCGATGCAACCGATGTAGTTGCTCTATCTGGTGCCCACACTTTTGGTCAAGCTCATTGTCCAACAATGTTCAACCGAATCATTGAATCCGACCCACCAATCGAACCAAATTTCAAGAAACAACTCGAAGCAACATGTCCGAATGAGGAGTCCCTCAACGCCGTTAATTTGGATGGTGTGTTTACATCGGATCAAGACTTGGCAAGTCATCCTAAGACTAAGGAGATTGTGAACCTCTTTGCTTCTAACCAGAAAGAGTTTTTTAACAAATTTGCTAATGCTTTTGTTAAGGTTAGTCAATTGGATGTTTTGACTGGAAATCAAGGAGAAATTCGTAAGTCATGTTTTGCTCCAAATAATAGGAAATCTAAGGTGGCATCTATGGTGGAAGAGGTGGTGGAAATTGCAGCAAACATGTAG
Protein sequence

>Msa0439000

MVKSLRNINVEFVPRCPLLRNIIFEGPDFDVPLGRKDSLNFSVNAGDNLPVPFARTDELLTVFGSKKFDATDVVALSGAHTFGQAHCPTMFNRIIESDPPIEPNFKKQLEATCPNEESLNAVNLDGVFTSDQDLASHPKTKEIVNLFASNQKEFFNKFANAFVKVSQLDVLTGNQGEIRKSCFAPNNRKSKVASMVEEVVEIAANM*