AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1365450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1365450 MtrunA17_Chr6g0467251 83.663 202 10 1 24 202 147 348 3.76e-110 318
Msa1365450 MtrunA17_Chr6g0467121 49.746 197 75 2 26 198 184 380 6.16e-57 184
Msa1365450 MtrunA17_Chr6g0467161 46.316 190 77 2 20 184 149 338 2.45e-50 166
Msa1365450 MtrunA17_Chr2g0292241 38.860 193 85 4 19 178 134 326 2.59e-37 132
Msa1365450 MtrunA17_Chr2g0292211 38.860 193 85 4 19 178 145 337 1.46e-36 130
Msa1365450 MtrunA17_Chr1g0188431 38.506 174 86 2 26 178 150 323 9.51e-34 122
Msa1365450 MtrunA17_Chr2g0291751 35.961 203 97 4 19 188 134 336 1.10e-33 123
Msa1365450 MtrunA17_Chr5g0423911 87.692 65 8 0 122 186 11 75 1.53e-33 115
Msa1365450 MtrunA17_Chr2g0292151 35.047 214 104 5 19 197 134 347 7.87e-33 120
Msa1365450 MtrunA17_Chr2g0292281 36.612 183 93 5 19 178 30 212 2.16e-32 117
Msa1365450 MtrunA17_Chr2g0292251 34.272 213 102 6 21 195 135 347 3.85e-32 119
Msa1365450 MtrunA17_Chr2g0292111 34.975 203 99 4 19 188 134 336 7.67e-32 118
Msa1365450 MtrunA17_Chr2g0292181 34.715 193 93 4 19 178 143 335 8.44e-31 115
Msa1365450 MtrunA17_Chr7g0254231 36.111 180 91 2 22 178 129 307 1.33e-29 111
Msa1365450 MtrunA17_Chr2g0292261 33.962 212 107 4 19 197 134 345 1.46e-29 112
Msa1365450 MtrunA17_Chr2g0292091 35.789 190 89 4 22 178 132 321 1.95e-29 111
Msa1365450 MtrunA17_Chr2g0292101 35.149 202 96 5 22 189 133 333 2.02e-29 111
Msa1365450 MtrunA17_Chr2g0292271 35.961 203 95 5 26 194 141 342 3.20e-29 111
Msa1365450 MtrunA17_Chr2g0292291 36.598 194 89 4 19 178 135 328 3.27e-29 111
Msa1365450 MtrunA17_Chr6g0477071 36.782 174 89 2 26 178 165 338 1.09e-28 109
Msa1365450 MtrunA17_Chr4g0013191 39.459 185 82 6 22 178 154 336 5.14e-28 107
Msa1365450 MtrunA17_Chr1g0197411 35.676 185 87 4 26 178 142 326 1.01e-26 104
Msa1365450 MtrunA17_Chr8g0364291 32.632 190 96 4 26 183 136 325 1.44e-26 103
Msa1365450 MtrunA17_Chr4g0013721 32.275 189 96 4 26 184 212 398 2.17e-26 104
Msa1365450 MtrunA17_Chr4g0013201 37.079 178 86 4 26 178 158 334 2.42e-26 103
Msa1365450 MtrunA17_Chr5g0402581 32.812 192 96 3 20 178 131 322 3.26e-26 102
Msa1365450 MtrunA17_Chr1g0175561 35.484 186 87 5 26 178 137 322 4.49e-26 102
Msa1365450 MtrunA17_Chr5g0405991 33.880 183 87 5 26 178 134 312 6.20e-26 101
Msa1365450 MtrunA17_Chr3g0128061 32.973 185 92 3 26 178 138 322 9.61e-26 101
Msa1365450 MtrunA17_Chr7g0225221 33.333 183 95 4 23 178 134 316 9.69e-26 101
Msa1365450 MtrunA17_Chr4g0072091 36.702 188 88 4 26 182 136 323 1.00e-25 101
Msa1365450 MtrunA17_Chr4g0075241 32.787 183 93 3 26 178 141 323 1.24e-25 101
Msa1365450 MtrunA17_Chr5g0404721 31.959 194 100 5 22 183 138 331 1.35e-25 101
Msa1365450 MtrunA17_Chr3g0129061 32.086 187 99 3 23 182 137 322 1.51e-25 100
Msa1365450 MtrunA17_Chr4g0013821 32.308 195 100 4 26 190 140 332 1.66e-25 101
Msa1365450 MtrunA17_Chr1g0164891 33.880 183 89 4 28 178 206 388 4.09e-25 100
Msa1365450 MtrunA17_Chr4g0046131 33.333 186 91 5 26 178 157 342 4.85e-25 99.8
Msa1365450 MtrunA17_Chr8g0371381 33.508 191 91 6 20 178 129 315 4.91e-25 99.4
Msa1365450 MtrunA17_Chr3g0114461 33.690 187 97 4 22 182 7 192 6.75e-25 96.3
Msa1365450 MtrunA17_Chr7g0244461 34.066 182 94 4 22 178 128 308 9.69e-25 98.6
Msa1365450 MtrunA17_Chr3g0129051 33.155 187 97 3 23 182 137 322 1.17e-24 98.6
Msa1365450 MtrunA17_Chr4g0013171 33.690 187 97 4 22 182 136 321 1.82e-24 97.8
Msa1365450 MtrunA17_Chr2g0320071 34.254 181 93 4 23 178 134 313 2.39e-24 97.4
Msa1365450 MtrunA17_Chr3g0114451 33.146 178 93 3 26 178 138 314 2.75e-24 97.4
Msa1365450 MtrunA17_Chr7g0268501 31.551 187 96 5 27 182 135 320 3.01e-24 97.4
Msa1365450 MtrunA17_Chr7g0268521 31.551 187 96 5 27 182 135 320 3.01e-24 97.4
Msa1365450 MtrunA17_Chr1g0148021 32.065 184 94 4 26 178 141 324 4.01e-24 97.1
Msa1365450 MtrunA17_Chr5g0402571 31.633 196 101 3 20 182 131 326 4.15e-24 97.1
Msa1365450 MtrunA17_Chr3g0106901 33.708 178 92 4 26 178 143 319 5.42e-24 96.7
Msa1365450 MtrunA17_Chr7g0224371 32.044 181 98 4 22 178 139 318 6.80e-24 96.3
Msa1365450 MtrunA17_Chr5g0438021 31.016 187 101 3 23 182 140 325 6.12e-23 94.0
Msa1365450 MtrunA17_Chr2g0320101 33.702 181 94 4 23 178 134 313 8.55e-23 93.6
Msa1365450 MtrunA17_Chr5g0400331 32.461 191 90 6 26 178 138 327 1.03e-22 93.6
Msa1365450 MtrunA17_Chr1g0194151 31.915 188 95 3 28 182 146 333 1.90e-22 92.8
Msa1365450 MtrunA17_Chr2g0299691 31.746 189 93 4 26 178 166 354 4.50e-22 92.0
Msa1365450 MtrunA17_Chr1g0157051 30.601 183 94 4 26 178 135 314 5.01e-22 91.3
Msa1365450 MtrunA17_Chr4g0024121 30.811 185 96 5 26 178 138 322 6.14e-22 91.3
Msa1365450 MtrunA17_Chr2g0320091 31.694 183 98 4 22 178 133 314 6.88e-22 90.9
Msa1365450 MtrunA17_Chr5g0432441 31.720 186 97 3 26 182 142 326 1.31e-21 90.5
Msa1365450 MtrunA17_Chr1g0213061 32.778 180 93 3 26 178 139 317 1.46e-21 90.1
Msa1365450 MtrunA17_Chr6g0451571 33.155 187 95 5 22 178 144 330 1.89e-21 90.1
Msa1365450 MtrunA17_Chr8g0372601 28.571 189 103 5 22 178 138 326 2.11e-21 89.7
Msa1365450 MtrunA17_Chr7g0254201 32.258 186 97 4 26 184 131 314 2.56e-21 89.4
Msa1365450 MtrunA17_Chr4g0013181 31.016 187 102 4 22 182 168 353 2.83e-21 89.7
Msa1365450 MtrunA17_Chr3g0106921 31.351 185 98 3 22 178 133 316 2.95e-21 89.4
Msa1365450 MtrunA17_Chr2g0322861 31.414 191 98 5 26 183 197 387 3.78e-21 89.7
Msa1365450 MtrunA17_Chr1g0155611 31.579 190 97 4 22 178 154 343 6.36e-21 88.6
Msa1365450 MtrunA17_Chr4g0068621 29.843 191 104 4 22 182 146 336 6.81e-21 88.6
Msa1365450 MtrunA17_Chr2g0320061 32.418 182 96 4 23 178 134 314 8.94e-21 87.8
Msa1365450 MtrunA17_Chr4g0038161 33.514 185 95 3 26 182 136 320 1.09e-20 87.8
Msa1365450 MtrunA17_Chr4g0053871 32.402 179 95 4 26 178 142 320 1.55e-20 87.4
Msa1365450 MtrunA17_Chr6g0460711 33.871 186 91 5 26 182 132 314 2.48e-20 86.7
Msa1365450 MtrunA17_Chr4g0038151 32.432 185 97 3 26 182 137 321 4.53e-20 85.9
Msa1365450 MtrunA17_Chr1g0194271 31.795 195 93 5 28 182 146 340 4.94e-20 86.3
Msa1365450 MtrunA17_Chr3g0129041 30.481 187 102 3 23 182 137 322 8.00e-20 85.5
Msa1365450 MtrunA17_Chr7g0244451 32.796 186 99 4 22 182 132 316 8.62e-20 85.1
Msa1365450 MtrunA17_Chr7g0258811 31.915 188 91 6 26 178 182 367 1.60e-19 85.1
Msa1365450 MtrunA17_Chr2g0279041 30.769 195 101 4 22 182 131 325 3.30e-19 83.6
Msa1365450 MtrunA17_Chr3g0107881 32.973 185 94 6 23 178 137 320 3.51e-19 83.6
Msa1365450 MtrunA17_Chr2g0320051 32.418 182 96 4 23 178 134 314 4.83e-19 83.2
Msa1365450 MtrunA17_Chr4g0076211 31.551 187 98 5 21 178 144 329 6.27e-19 83.2
Msa1365450 MtrunA17_Chr5g0401431 30.435 184 97 3 26 178 142 325 7.03e-19 82.8
Msa1365450 MtrunA17_Chr1g0204011 28.962 183 98 3 26 178 138 318 7.78e-19 82.8
Msa1365450 MtrunA17_Chr3g0136751 31.606 193 99 3 23 182 130 322 8.78e-19 82.4
Msa1365450 MtrunA17_Chr5g0432351 32.386 176 92 5 29 178 143 317 9.91e-19 82.4
Msa1365450 MtrunA17_Chr5g0432341 32.386 176 92 5 29 178 143 317 1.59e-18 82.0
Msa1365450 MtrunA17_Chr4g0070951 29.442 197 104 4 22 183 134 330 3.99e-18 80.9
Msa1365450 MtrunA17_Chr4g0063671 30.688 189 96 4 25 178 128 316 5.27e-18 80.5
Msa1365450 MtrunA17_Chr2g0320031 30.220 182 100 4 23 178 135 315 6.99e-18 80.1
Msa1365450 MtrunA17_Chr4g0074851 29.255 188 104 4 26 184 143 330 1.58e-17 79.3
Msa1365450 MtrunA17_Chr2g0279061 29.744 195 103 4 22 182 72 266 3.70e-17 77.4
Msa1365450 MtrunA17_Chr4g0043391 33.333 189 91 6 26 182 139 324 5.31e-17 77.8
Msa1365450 MtrunA17_Chr1g0181511 27.568 185 105 4 23 178 135 319 5.47e-17 77.8
Msa1365450 MtrunA17_Chr4g0038171 33.333 189 91 6 26 182 136 321 5.84e-17 77.4
Msa1365450 MtrunA17_Chr1g0181431 28.022 182 102 3 26 178 138 319 1.13e-16 76.6
Msa1365450 MtrunA17_Chr2g0296621 30.939 181 96 7 26 178 158 337 3.15e-16 75.5
Msa1365450 MtrunA17_Chr5g0432331 30.114 176 96 5 29 178 174 348 1.23e-15 73.9
Msa1365450 MtrunA17_Chr2g0278671 29.231 195 104 3 22 182 129 323 1.36e-15 73.6
Msa1365450 MtrunA17_Chr1g0181481 26.486 185 107 3 23 178 135 319 2.84e-15 72.8
Msa1365450 MtrunA17_Chr1g0181501 27.624 181 101 4 27 178 6 185 3.84e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1365450 AT1G71695.1 39.614 207 97 4 22 202 153 357 1.05e-40 142
Msa1365450 AT4G33420.1 36.022 186 96 2 20 182 140 325 6.78e-32 118
Msa1365450 AT4G33420.2 36.022 186 96 2 20 182 187 372 1.61e-31 118
Msa1365450 AT4G08770.1 35.185 216 104 7 22 202 132 346 1.61e-30 114
Msa1365450 AT5G19880.1 36.898 187 84 4 26 178 139 325 2.42e-28 108
Msa1365450 AT2G22420.1 34.392 189 92 5 26 184 135 321 1.75e-27 106
Msa1365450 AT4G08780.1 33.333 216 108 7 22 202 132 346 2.94e-27 106
Msa1365450 AT4G37520.2 33.511 188 94 4 26 182 139 326 4.67e-27 105
Msa1365450 AT4G37520.1 33.511 188 94 4 26 182 142 329 5.16e-27 105
Msa1365450 AT2G18150.1 31.959 194 100 5 22 183 144 337 2.25e-26 103
Msa1365450 AT2G18980.1 34.574 188 92 4 22 178 132 319 2.55e-26 103
Msa1365450 AT5G40150.1 34.783 184 93 3 26 182 145 328 3.84e-26 102
Msa1365450 AT5G14130.1 32.984 191 98 4 22 182 140 330 4.87e-26 102
Msa1365450 AT5G14130.2 32.984 191 98 4 22 182 181 371 6.78e-26 102
Msa1365450 AT3G28200.1 33.690 187 98 3 22 182 130 316 1.28e-25 101
Msa1365450 AT4G36430.1 32.105 190 97 4 21 178 137 326 1.42e-25 101
Msa1365450 AT3G01190.1 31.414 191 100 4 22 182 132 321 1.59e-25 101
Msa1365450 AT5G06730.1 33.673 196 97 4 26 188 145 340 7.40e-25 100
Msa1365450 AT5G15180.1 31.915 188 97 4 22 178 138 325 8.34e-25 99.4
Msa1365450 AT5G17820.1 34.078 179 91 3 26 178 132 309 1.19e-24 98.6
Msa1365450 AT5G17820.2 34.078 179 91 3 26 178 185 362 2.89e-24 98.6
Msa1365450 AT4G37530.1 32.447 188 96 4 26 182 142 329 3.87e-24 97.4
Msa1365450 AT3G03670.1 33.673 196 97 4 20 182 126 321 5.51e-24 97.1
Msa1365450 AT2G18140.1 31.282 195 102 5 21 183 142 336 6.85e-24 97.1
Msa1365450 AT4G30170.1 31.915 188 97 3 22 178 134 321 8.10e-24 96.7
Msa1365450 AT2G38390.1 35.025 197 94 7 22 184 139 335 1.13e-23 96.7
Msa1365450 AT5G19890.1 32.143 196 97 4 22 183 135 328 1.15e-23 96.3
Msa1365450 AT3G17070.2 32.796 186 92 4 26 178 87 272 1.40e-23 95.1
Msa1365450 AT3G32980.1 31.731 208 102 7 26 198 143 345 2.30e-23 95.9
Msa1365450 AT2G38380.1 34.715 193 92 6 26 184 143 335 2.50e-23 95.5
Msa1365450 AT3G17070.1 32.796 186 92 4 26 178 150 335 3.08e-23 95.1
Msa1365450 AT5G66390.1 30.159 189 100 4 22 178 141 329 4.60e-23 94.7
Msa1365450 AT5G42180.1 32.240 183 91 4 26 178 134 313 5.80e-23 94.4
Msa1365450 AT4G11290.1 31.414 191 96 6 22 178 133 322 1.16e-22 93.6
Msa1365450 AT1G05240.1 35.233 193 92 6 23 182 133 325 1.22e-22 93.6
Msa1365450 AT1G05250.1 35.233 193 92 6 23 182 133 325 1.22e-22 93.6
Msa1365450 AT5G64120.1 31.937 191 99 4 22 182 139 328 2.18e-22 92.8
Msa1365450 AT1G24110.1 31.414 191 100 5 26 185 135 325 2.24e-22 92.8
Msa1365450 AT1G44970.1 32.474 194 98 4 22 182 153 346 2.28e-22 93.2
Msa1365450 AT5G58400.1 31.746 189 100 4 22 182 138 325 3.54e-22 92.0
Msa1365450 AT1G14540.1 32.240 183 98 4 26 182 133 315 7.91e-22 91.3
Msa1365450 AT5G58390.1 30.159 189 103 3 22 182 129 316 1.10e-21 90.9
Msa1365450 AT5G06720.1 32.258 186 93 4 26 178 144 329 1.22e-21 90.9
Msa1365450 AT3G50990.1 30.159 189 100 6 22 178 149 337 1.37e-21 90.9
Msa1365450 AT4G26010.1 33.149 181 95 5 26 183 133 310 1.57e-21 90.1
Msa1365450 AT4G26010.2 33.149 181 95 5 26 183 161 338 1.69e-21 90.5
Msa1365450 AT3G49110.1 31.250 208 103 7 26 198 145 347 1.91e-21 90.5
Msa1365450 AT3G49120.1 31.250 208 103 7 26 198 144 346 2.01e-21 90.5
Msa1365450 AT5G67400.1 32.275 189 97 4 25 182 141 329 6.09e-21 89.0
Msa1365450 AT5G24070.1 31.414 191 95 4 27 182 146 335 6.47e-21 89.0
Msa1365450 AT1G49570.1 31.959 194 98 4 22 181 156 349 8.69e-21 89.0
Msa1365450 AT2G24800.1 27.753 227 118 6 1 182 104 329 8.97e-21 88.6
Msa1365450 AT4G17690.1 31.111 180 97 3 26 178 139 318 2.44e-20 87.4
Msa1365450 AT5G05340.1 29.787 188 104 3 22 182 138 324 3.08e-20 87.0
Msa1365450 AT2G39040.1 31.937 191 93 7 24 178 157 346 3.19e-20 87.0
Msa1365450 AT1G05260.1 28.866 194 99 6 26 183 136 326 1.48e-19 85.1
Msa1365450 AT1G14550.1 32.044 181 94 5 26 178 138 317 1.78e-19 84.7
Msa1365450 AT2G41480.1 31.746 189 94 4 25 178 136 324 1.82e-19 84.7
Msa1365450 AT5G51890.1 31.492 181 91 4 26 175 137 315 2.48e-19 84.3
Msa1365450 AT5G47000.1 31.183 186 100 4 26 183 146 331 5.34e-19 83.6
Msa1365450 AT3G49960.1 31.915 188 97 5 26 182 142 329 5.57e-19 83.6
Msa1365450 AT1G30870.1 32.984 191 94 7 26 184 159 347 7.78e-19 83.6
Msa1365450 AT3G21770.1 29.101 189 95 6 26 178 139 324 1.88e-18 82.0
Msa1365450 AT1G34510.1 32.967 182 94 6 26 183 133 310 4.86e-18 80.9
Msa1365450 AT5G39580.1 32.632 190 92 5 26 182 133 319 7.97e-18 80.1
Msa1365450 AT4G31760.1 26.842 190 106 4 26 182 137 326 3.10e-17 78.6
Msa1365450 AT2G34060.1 28.495 186 100 4 26 178 157 342 3.85e-17 78.6
Msa1365450 AT1G77100.1 29.947 187 102 3 25 182 133 319 9.20e-17 77.4
Msa1365450 AT2G37130.2 26.203 187 108 5 22 178 106 292 7.29e-16 74.7
Msa1365450 AT4G21960.1 28.571 182 101 4 26 178 142 323 8.95e-16 74.7
Msa1365450 AT2G37130.1 26.203 187 108 5 22 178 137 323 9.30e-16 74.7
Msa1365450 AT4G25980.1 27.604 192 104 3 26 182 135 326 9.89e-16 74.7
Msa1365450 AT5G64110.1 30.000 190 99 5 26 182 142 330 2.06e-14 70.9
Msa1365450 AT2G43480.1 26.596 188 102 4 26 178 145 331 2.41e-14 70.9
Msa1365450 AT4G37530.2 37.500 96 53 2 26 114 142 237 8.79e-14 68.6
Msa1365450 AT5G22410.1 26.596 188 103 5 29 183 141 326 7.67e-13 66.2

Find 42 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTAGATTTCCTATTATT+TGG 0.128939 tig0018294:-152323 Msa1365450:intergenic
TCGAGTTGTTTCTTGAAATT+TGG 0.165142 tig0018294:-152020 Msa1365450:intergenic
CTATCTGGTGCCCACACTTT+TGG 0.251851 tig0018294:+151939 Msa1365450:CDS
CCCAATGGCACGTCAAAATC+TGG 0.255614 tig0018294:-151792 Msa1365450:intergenic
AATATTATGTTACGCAATAA+AGG 0.298757 tig0018294:-151762 Msa1365450:intergenic
CTGCATTTACACTGAAGTTT+AGG 0.301903 tig0018294:-151827 Msa1365450:intergenic
GAAATCTAAGGTGGCATCTA+TGG 0.324726 tig0018294:+152334 Msa1365450:CDS
TTCACAATCTCCTTAGTCTT+AGG 0.327909 tig0018294:-152182 Msa1365450:intergenic
TCCAAATAATAGGAAATCTA+AGG 0.367020 tig0018294:+152322 Msa1365450:CDS
ATGATTCGGTTGAACATTGT+TGG 0.380602 tig0018294:-151975 Msa1365450:intergenic
ACCGATGTAGTTGCTCTATC+TGG 0.387253 tig0018294:+151924 Msa1365450:CDS
TCTGTTCGTGCAAATGGTAC+TGG 0.412087 tig0018294:-151861 Msa1365450:intergenic
AGTTCATCTGTTCGTGCAAA+TGG 0.412175 tig0018294:-151867 Msa1365450:intergenic
TTGAAATTTGGTTCGATTGG+TGG 0.414542 tig0018294:-152008 Msa1365450:intergenic
TTGCGTAACATAATATTTGA+AGG 0.420033 tig0018294:+151768 Msa1365450:CDS
GTGGGTCGGATTCAATGATT+CGG 0.450356 tig0018294:-151989 Msa1365450:intergenic
TTCTTGAAATTTGGTTCGAT+TGG 0.452274 tig0018294:-152011 Msa1365450:intergenic
TGAAATTTGGTTCGATTGGT+GGG 0.456433 tig0018294:-152007 Msa1365450:intergenic
GGCAAGTCATCCTAAGACTA+AGG 0.457751 tig0018294:+152172 Msa1365450:CDS
ATCCAAATTAACAGCGTTGA+GGG 0.485872 tig0018294:-152066 Msa1365450:intergenic
CTAAACTTCAGTGTAAATGC+AGG 0.486224 tig0018294:+151828 Msa1365450:CDS
ATTTGGTTCGATTGGTGGGT+CGG 0.494826 tig0018294:-152003 Msa1365450:intergenic
CCAATGGCACGTCAAAATCT+GGG 0.508698 tig0018294:-151791 Msa1365450:intergenic
CTGTTCGTGCAAATGGTACT+GGG 0.511334 tig0018294:-151860 Msa1365450:intergenic
ATCTATGGTGGAAGAGGTGG+TGG 0.516164 tig0018294:+152349 Msa1365450:CDS
GCGTTGAGGGACTCCTCATT+CGG 0.516346 tig0018294:-152053 Msa1365450:intergenic
GGTGGCATCTATGGTGGAAG+AGG 0.527491 tig0018294:+152343 Msa1365450:CDS
GGCATCTATGGTGGAAGAGG+TGG 0.536043 tig0018294:+152346 Msa1365450:CDS
CTTTCTGGTTAGAAGCAAAG+AGG 0.545522 tig0018294:-152205 Msa1365450:intergenic
GTTTACATCGGATCAAGACT+TGG 0.546163 tig0018294:+152151 Msa1365450:CDS
CGAAGCAACATGTCCGAATG+AGG 0.556767 tig0018294:+152040 Msa1365450:CDS
ATCTAAGGTGGCATCTATGG+TGG 0.582875 tig0018294:+152337 Msa1365450:CDS
TAAACTTCAGTGTAAATGCA+GGG 0.589272 tig0018294:+151829 Msa1365450:CDS
AATGAGCTTGACCAAAAGTG+TGG 0.611586 tig0018294:-151950 Msa1365450:intergenic
CATCCAAATTAACAGCGTTG+AGG 0.638233 tig0018294:-152067 Msa1365450:intergenic
AAATAATAGGAAATCTAAGG+TGG 0.648910 tig0018294:+152325 Msa1365450:CDS
ATGAGCTTGACCAAAAGTGT+GGG 0.655019 tig0018294:-151949 Msa1365450:intergenic
AAACTTCAGTGTAAATGCAG+GGG 0.666960 tig0018294:+151830 Msa1365450:CDS
TCACCAGGGTGTGTTTACAT+CGG 0.667869 tig0018294:+152139 Msa1365450:intron
ACCAGATAGAGCAACTACAT+CGG 0.673269 tig0018294:-151925 None:intergenic
TACGCAATAAAGGACATCGA+GGG 0.715776 tig0018294:-151752 None:intergenic
TTACGCAATAAAGGACATCG+AGG 0.746500 tig0018294:-151753 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTATGTTACGCAATAA+AGG - tig0018294:151765-151784 Msa1345140:intergenic 20.0%
!!! AAAATTTTTTGATCCGAAAA+CGG - tig0018294:151898-151917 Msa1365450:intergenic 20.0%
!!! ATTTGTTAAAAAACTCTTTC+TGG - tig0018294:152223-152242 Msa1365450:intergenic 20.0%
! TTGCGTAACATAATATTTGA+AGG + tig0018294:151768-151787 Msa1365450:CDS 25.0%
! TACATATTATCGAATGTGTT+TGG - tig0018294:152101-152120 Msa1365450:intergenic 25.0%
!!! ATTTGCTAATGCTTTTGTTA+AGG + tig0018294:152241-152260 Msa1365450:CDS 25.0%
!!! TTTTGTTAAGGTTAGTCAAT+TGG + tig0018294:152253-152272 Msa1365450:CDS 25.0%
! TCCAAATAATAGGAAATCTA+AGG + tig0018294:152322-152341 Msa1365450:CDS 25.0%
! ACCTTAGATTTCCTATTATT+TGG - tig0018294:152326-152345 Msa1365450:intergenic 25.0%
! AAATAATAGGAAATCTAAGG+TGG + tig0018294:152325-152344 Msa1365450:CDS 25.0%
TAAACTTCAGTGTAAATGCA+GGG + tig0018294:151829-151848 Msa1365450:CDS 30.0%
! GATGAACTATTAACCGTTTT+CGG + tig0018294:151882-151901 Msa1365450:CDS 30.0%
! TTCTTGAAATTTGGTTCGAT+TGG - tig0018294:152014-152033 Msa1365450:intergenic 30.0%
!! TCGAGTTGTTTCTTGAAATT+TGG - tig0018294:152023-152042 Msa1365450:intergenic 30.0%
! CATGTTTTGCTCCAAATAAT+AGG + tig0018294:152312-152331 Msa1365450:CDS 30.0%
CTGCATTTACACTGAAGTTT+AGG - tig0018294:151830-151849 Msa1365450:intergenic 35.0%
CTAAACTTCAGTGTAAATGC+AGG + tig0018294:151828-151847 Msa1365450:CDS 35.0%
AAACTTCAGTGTAAATGCAG+GGG + tig0018294:151830-151849 Msa1365450:CDS 35.0%
!! ATGATTCGGTTGAACATTGT+TGG - tig0018294:151978-151997 Msa1365450:intergenic 35.0%
! TGAAATTTGGTTCGATTGGT+GGG - tig0018294:152010-152029 Msa1365450:intergenic 35.0%
! TTGAAATTTGGTTCGATTGG+TGG - tig0018294:152011-152030 Msa1365450:intergenic 35.0%
! ATCCAAATTAACAGCGTTGA+GGG - tig0018294:152069-152088 Msa1365450:intergenic 35.0%
ATTAACCTCTTGAATCACCA+GGG + tig0018294:152125-152144 Msa1365450:intron 35.0%
! TTCACAATCTCCTTAGTCTT+AGG - tig0018294:152185-152204 Msa1365450:intergenic 35.0%
!!! AGTCAATTGGATGTTTTGAC+TGG + tig0018294:152266-152285 Msa1365450:CDS 35.0%
!!! GATGTTTTGACTGGAAATCA+AGG + tig0018294:152275-152294 Msa1365450:CDS 35.0%
TACGCAATAAAGGACATCGA+GGG - tig0018294:151755-151774 Msa1345140:intergenic 40.0%
TTACGCAATAAAGGACATCG+AGG - tig0018294:151756-151775 Msa1345140:intergenic 40.0%
AGTTCATCTGTTCGTGCAAA+TGG - tig0018294:151870-151889 Msa1365450:intergenic 40.0%
ACCAGATAGAGCAACTACAT+CGG - tig0018294:151928-151947 Msa1365450:intergenic 40.0%
! ATGAGCTTGACCAAAAGTGT+GGG - tig0018294:151952-151971 Msa1365450:intergenic 40.0%
AATGAGCTTGACCAAAAGTG+TGG - tig0018294:151953-151972 Msa1365450:intergenic 40.0%
CATCCAAATTAACAGCGTTG+AGG - tig0018294:152070-152089 Msa1365450:intergenic 40.0%
CATTAACCTCTTGAATCACC+AGG + tig0018294:152124-152143 Msa1365450:intron 40.0%
GTTTACATCGGATCAAGACT+TGG + tig0018294:152151-152170 Msa1365450:CDS 40.0%
!! CTTTCTGGTTAGAAGCAAAG+AGG - tig0018294:152208-152227 Msa1365450:intergenic 40.0%
! GAAATCTAAGGTGGCATCTA+TGG + tig0018294:152334-152353 Msa1365450:CDS 40.0%
CCAATGGCACGTCAAAATCT+GGG - tig0018294:151794-151813 Msa1365450:intergenic 45.0%
!! CCAGATTTTGACGTGCCATT+GGG + tig0018294:151792-151811 Msa1365450:CDS 45.0%
! AGGCTGTCTTTTCTTCCCAA+TGG - tig0018294:151810-151829 Msa1365450:intergenic 45.0%
CTGTTCGTGCAAATGGTACT+GGG - tig0018294:151863-151882 Msa1365450:intergenic 45.0%
TCTGTTCGTGCAAATGGTAC+TGG - tig0018294:151864-151883 Msa1365450:intergenic 45.0%
ACCGATGTAGTTGCTCTATC+TGG + tig0018294:151924-151943 Msa1365450:CDS 45.0%
GTGGGTCGGATTCAATGATT+CGG - tig0018294:151992-152011 Msa1365450:intergenic 45.0%
! ATTTGGTTCGATTGGTGGGT+CGG - tig0018294:152006-152025 Msa1365450:intergenic 45.0%
GTCCCTCAACGCTGTTAATT+TGG + tig0018294:152064-152083 Msa1365450:intron 45.0%
TCACCAGGGTGTGTTTACAT+CGG + tig0018294:152139-152158 Msa1365450:intron 45.0%
GGCAAGTCATCCTAAGACTA+AGG + tig0018294:152172-152191 Msa1365450:CDS 45.0%
! ATCTAAGGTGGCATCTATGG+TGG + tig0018294:152337-152356 Msa1365450:CDS 45.0%
!! CCCAGATTTTGACGTGCCAT+TGG + tig0018294:151791-151810 Msa1365450:CDS 50.0%
CCCAATGGCACGTCAAAATC+TGG - tig0018294:151795-151814 Msa1365450:intergenic 50.0%
! CTATCTGGTGCCCACACTTT+TGG + tig0018294:151939-151958 Msa1365450:CDS 50.0%
CGAAGCAACATGTCCGAATG+AGG + tig0018294:152040-152059 Msa1365450:CDS 50.0%
ACACACCCTGGTGATTCAAG+AGG - tig0018294:152133-152152 Msa1365450:intergenic 50.0%
GATCCGATGTAAACACACCC+TGG - tig0018294:152145-152164 Msa1365450:intergenic 50.0%
! ATCTATGGTGGAAGAGGTGG+TGG + tig0018294:152349-152368 Msa1365450:CDS 50.0%
GCGTTGAGGGACTCCTCATT+CGG - tig0018294:152056-152075 Msa1365450:intergenic 55.0%
! GGTGGCATCTATGGTGGAAG+AGG + tig0018294:152343-152362 Msa1365450:CDS 55.0%
! GGCATCTATGGTGGAAGAGG+TGG + tig0018294:152346-152365 Msa1365450:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0018294 gene 151714 152391 151714 ID=Msa1365450;Name=Msa1365450
tig0018294 mRNA 151714 152391 151714 ID=Msa1365450-mRNA-1;Parent=Msa1365450;Name=Msa1365450-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0|0|1|1|1|2|0|202
tig0018294 exon 151714 152076 151714 ID=Msa1365450-mRNA-1:exon:14568;Parent=Msa1365450-mRNA-1
tig0018294 exon 152146 152391 152146 ID=Msa1365450-mRNA-1:exon:14569;Parent=Msa1365450-mRNA-1
tig0018294 CDS 151714 152076 151714 ID=Msa1365450-mRNA-1:cds;Parent=Msa1365450-mRNA-1
tig0018294 CDS 152146 152391 152146 ID=Msa1365450-mRNA-1:cds;Parent=Msa1365450-mRNA-1
Gene Sequence

>Msa1365450

ATGGTTAAAAGTTTGAGAAATATTAATGTTGAATTTGTCCCTCGATGTCCTTTATTGCGTAACATAATATTTGAAGGCCCAGATTTTGACGTGCCATTGGGAAGAAAAGACAGCCTAAACTTCAGTGTAAATGCAGGGGATAATCTCCCAGTACCATTTGCACGAACAGATGAACTATTAACCGTTTTCGGATCAAAAAATTTTGATGCAACCGATGTAGTTGCTCTATCTGGTGCCCACACTTTTGGTCAAGCTCATTGTCCAACAATGTTCAACCGAATCATTGAATCCGACCCACCAATCGAACCAAATTTCAAGAAACAACTCGAAGCAACATGTCCGAATGAGGAGTCCCTCAACGCTGGTGTGTTTACATCGGATCAAGACTTGGCAAGTCATCCTAAGACTAAGGAGATTGTGAACCTCTTTGCTTCTAACCAGAAAGAGTTTTTTAACAAATTTGCTAATGCTTTTGTTAAGGTTAGTCAATTGGATGTTTTGACTGGAAATCAAGGAGAAATTCGTAAGTCATGTTTTGCTCCAAATAATAGGAAATCTAAGGTGGCATCTATGGTGGAAGAGGTGGTGGAAATTGCAGCAAACATGTAG
Protein sequence

>Msa1365450

MVKSLRNINVEFVPRCPLLRNIIFEGPDFDVPLGRKDSLNFSVNAGDNLPVPFARTDELLTVFGSKNFDATDVVALSGAHTFGQAHCPTMFNRIIESDPPIEPNFKKQLEATCPNEESLNAGVFTSDQDLASHPKTKEIVNLFASNQKEFFNKFANAFVKVSQLDVLTGNQGEIRKSCFAPNNRKSKVASMVEEVVEIAANM*