AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1214240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1214240 MtrunA17_Chr4g0061861 93.496 246 16 0 1 246 4 249 4.39e-174 479
Msa1214240 MtrunA17_Chr4g0062071 92.276 246 16 1 1 246 1 243 1.29e-156 434
Msa1214240 MtrunA17_Chr4g0062041 88.889 36 4 0 201 236 2 37 2.04e-15 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1214240 AT5G35520.1 42.213 244 123 7 1 239 1 231 5.24e-55 177

Find 87 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGTCTTCTCAATTTAAT+TGG 0.103016 8_1:+74739489 Msa1214240:three_prime_UTR
CCCTTTACATATTCCCCTTT+TGG 0.156078 8_1:-74739682 None:intergenic
GAAATTTCGGGAATCGAATT+TGG 0.161069 8_1:+74737193 Msa1214240:five_prime_UTR
CTCTTACGCGGAGTTGTTTC+AGG 0.161244 8_1:+74738464 Msa1214240:CDS
ATAGTTGTTTACAGCTTTAT+TGG 0.213232 8_1:+74739739 Msa1214240:three_prime_UTR
GTACGGTTGGACTGAAATTT+CGG 0.259504 8_1:+74737180 Msa1214240:five_prime_UTR
TATTGGTCTATTTGTTGAAT+TGG 0.272032 8_1:+74739756 Msa1214240:three_prime_UTR
AAGGATTGACAACAATAAAA+TGG 0.285180 8_1:+74739177 Msa1214240:CDS
TACGGTTGGACTGAAATTTC+GGG 0.288547 8_1:+74737181 Msa1214240:five_prime_UTR
TACCGCTGCAGTATTATTTA+CGG 0.289167 8_1:-74739976 None:intergenic
TGTAATATTCTTTAATATAG+TGG 0.290128 8_1:-74740085 None:intergenic
TCAGAAGGTTCAATCTGTTT+TGG 0.291774 8_1:+74737642 Msa1214240:CDS
CTGCAGCGGTAGCAATAAAA+TGG 0.299321 8_1:+74739988 Msa1214240:three_prime_UTR
CATTGTTAGCTGAGTTTACT+AGG 0.310089 8_1:+74739614 Msa1214240:three_prime_UTR
GGATCAAAAGCTCGCTGTTT+GGG 0.327066 8_1:+74737663 Msa1214240:CDS
TCCGGTCTCTTCAATCTTGT+TGG 0.331304 8_1:-74739140 None:intergenic
TTGTGTTCCTGCAGAGATTA+TGG 0.353919 8_1:+74739075 Msa1214240:intron
ATAGAATTAGAAATAGTCTT+TGG 0.355229 8_1:-74737153 None:intergenic
TCCTGGTTCTACTTCCAATC+TGG 0.359101 8_1:+74738180 Msa1214240:CDS
AATCACTATATTGTATCTCT+TGG 0.361668 8_1:-74740122 None:intergenic
GAACATACATTGAGAGTGTT+TGG 0.361941 8_1:-74737736 None:intergenic
TCCAAAGTAAAATACATTAC+AGG 0.366210 8_1:+74739548 Msa1214240:three_prime_UTR
GGAATCGAATTTGGCGGGAA+TGG 0.369873 8_1:+74737202 Msa1214240:exon
CATTTCAAATGTATGGTACT+TGG 0.380857 8_1:+74739846 Msa1214240:three_prime_UTR
TTGAATTGGTTCTTAATTGA+TGG 0.382931 8_1:+74739770 Msa1214240:three_prime_UTR
TCTGTCTTCTCAATTTAATT+GGG 0.386873 8_1:+74739490 Msa1214240:three_prime_UTR
CATCAAAGATTTAGCAATCC+AGG 0.391347 8_1:-74739649 None:intergenic
TGGATCAAAAGCTCGCTGTT+TGG 0.391728 8_1:+74737662 Msa1214240:CDS
TTTATTCGTAGTCTATTAGT+TGG 0.395060 8_1:+74739428 Msa1214240:three_prime_UTR
TCAGAACTTCGACTCAAGAT+AGG 0.430690 8_1:+74739106 Msa1214240:CDS
AACCACTCATTTCAAATGTA+TGG 0.432061 8_1:+74739839 Msa1214240:three_prime_UTR
TGATGAGATTTCTGCCCAAA+AGG 0.434675 8_1:+74739667 Msa1214240:three_prime_UTR
TAAAATAATAGCATTAGAAT+AGG 0.447645 8_1:-74740160 None:intergenic
TAATTCTATCATTGTACGGT+TGG 0.453142 8_1:+74737167 Msa1214240:five_prime_UTR
ATCACTATATTGTATCTCTT+GGG 0.462214 8_1:-74740121 None:intergenic
AGTAGCTTCCGCATTGAGCT+TGG 0.467482 8_1:-74737463 None:intergenic
TCTGCTATATATGCTTCTAA+AGG 0.478873 8_1:+74739205 Msa1214240:CDS
AGCGAATCTCTACTTTCTAA+AGG 0.484540 8_1:-74739877 None:intergenic
CTGAGCATCTAATTCCAGAT+TGG 0.485974 8_1:-74738194 None:intergenic
TCGAATTTGGCGGGAATGGA+AGG 0.492575 8_1:+74737206 Msa1214240:exon
TTTCTAATTCTATCATTGTA+CGG 0.497693 8_1:+74737163 Msa1214240:five_prime_UTR
CCTTCTCATGCTGAAATGAG+TGG 0.498005 8_1:+74739456 Msa1214240:three_prime_UTR
CCCAAAAGGGGAATATGTAA+AGG 0.498189 8_1:+74739681 Msa1214240:three_prime_UTR
TAAAATACATTACAGGTCAC+AGG 0.498412 8_1:+74739555 Msa1214240:three_prime_UTR
ATGAAACCTGTGTGAGATGT+TGG 0.501041 8_1:-74737494 None:intergenic
CCAAAAGGGGAATATGTAAA+GGG 0.502598 8_1:+74739682 Msa1214240:three_prime_UTR
GATGAGATTTCTGCCCAAAA+GGG 0.504314 8_1:+74739668 Msa1214240:three_prime_UTR
AGTGGGAATGTCATTGATCC+TGG 0.506246 8_1:+74738163 Msa1214240:CDS
AGCTTCCGCATTGAGCTTGG+TGG 0.510752 8_1:-74737460 None:intergenic
TCTTACGCGGAGTTGTTTCA+GGG 0.514764 8_1:+74738465 Msa1214240:CDS
TTTGGCAATTGAAAACCGTC+AGG 0.516197 8_1:-74737718 None:intergenic
TGTTGATTGCATTCGTCAGA+AGG 0.518478 8_1:+74737627 Msa1214240:CDS
TGAAACCTGTGTGAGATGTT+GGG 0.524390 8_1:-74737493 None:intergenic
TTTCGGGAATCGAATTTGGC+GGG 0.533214 8_1:+74737197 Msa1214240:five_prime_UTR
ACTTCGTTGAAGAAGAGTTG+TGG 0.543092 8_1:-74737281 None:intergenic
AGAATAGGAGTGAGTCTCAC+AGG 0.550532 8_1:-74740145 None:intergenic
CTTCAAATTTCGTATTAGAG+AGG 0.553512 8_1:-74739314 None:intergenic
TATCTTGAGTCGAAGTTCTG+AGG 0.553557 8_1:-74739104 None:intergenic
TCCAGATTGGAAGTAGAACC+AGG 0.560356 8_1:-74738181 None:intergenic
ATTTCGGGAATCGAATTTGG+CGG 0.561030 8_1:+74737196 Msa1214240:five_prime_UTR
ATCAAAGATTTAGCAATCCA+GGG 0.563272 8_1:-74739648 None:intergenic
TACCATACATTTGAAATGAG+TGG 0.565072 8_1:-74739841 None:intergenic
ATGCTCAGTTAGAATCATTG+AGG 0.569791 8_1:+74738209 Msa1214240:CDS
TTGAGTAAATCCAGAGAGTG+TGG 0.570144 8_1:-74740229 None:intergenic
TTCTTTCAGGGTGAAACAAG+TGG 0.571772 8_1:+74738145 Msa1214240:intron
ACTAGGTTGTGATAAAGCCC+TGG 0.575518 8_1:+74739631 Msa1214240:three_prime_UTR
ACCAACAAGATTGAAGAGAC+CGG 0.579940 8_1:+74739139 Msa1214240:CDS
GTCAGTCTTCTCACAATGCT+CGG 0.580367 8_1:+74738406 Msa1214240:CDS
CCACTCATTTCAGCATGAGA+AGG 0.582573 8_1:-74739456 None:intergenic
GACTACGAATAAACAATCAC+AGG 0.584700 8_1:-74739418 None:intergenic
TTCTCAATTTAATTGGGCGA+AGG 0.594361 8_1:+74739496 Msa1214240:three_prime_UTR
ATGAGATTTCTGCCCAAAAG+GGG 0.597012 8_1:+74739669 Msa1214240:three_prime_UTR
TCTTTCAGGGTGAAACAAGT+GGG 0.606547 8_1:+74738146 Msa1214240:intron
TAAACAACTATTCCTTAACA+TGG 0.625217 8_1:-74739727 None:intergenic
TGAGTAAATCCAGAGAGTGT+GGG 0.631248 8_1:-74740228 None:intergenic
TGGGCGAAGGCAGAAGCTAG+AGG 0.634144 8_1:+74739509 Msa1214240:three_prime_UTR
CTTGAGTCGAAGTTCTGAGG+CGG 0.647508 8_1:-74739101 None:intergenic
GGAAAACCCTCTAATACCGA+AGG 0.648586 8_1:+74740029 Msa1214240:three_prime_UTR
AAAATACATTACAGGTCACA+GGG 0.652192 8_1:+74739556 Msa1214240:three_prime_UTR
TTCCGTAAATAATACTGCAG+CGG 0.655344 8_1:+74739974 Msa1214240:three_prime_UTR
ATTTGAGCAAAACTCTTACG+CGG 0.660438 8_1:+74738452 Msa1214240:CDS
AGTAGAAATCGAAAGTATCG+AGG 0.666017 8_1:-74737328 None:intergenic
TGGAACACCAAGATTTAACG+TGG 0.683567 8_1:+74740008 Msa1214240:three_prime_UTR
AAGATCCCAACATCTCACAC+AGG 0.719465 8_1:+74737488 Msa1214240:CDS
AGCATCCACCAAGCTCAATG+CGG 0.721723 8_1:+74737455 Msa1214240:CDS
TTATAGAGAAGATACCCACC+CGG 0.731734 8_1:+74740196 Msa1214240:three_prime_UTR
CAATGCTCGGTATATCAACG+AGG 0.745621 8_1:+74738419 Msa1214240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTTAATTAACTTTAATT+AGG + chr8_1:74737553-74737572 Msa1214240:intron 10.0%
!! TAAAAATGTATTATATGATG+TGG + chr8_1:74738000-74738019 Msa1214240:intron 15.0%
!! CTTAAGAAAAAATTATACAA+CGG - chr8_1:74738115-74738134 None:intergenic 15.0%
!!! AATATTTTACTAGCAAATAA+TGG + chr8_1:74738734-74738753 Msa1214240:intron 15.0%
!!! TAGAACAATAAATGATATTT+TGG - chr8_1:74739234-74739253 None:intergenic 15.0%
!!! ATGTTTAGAAATAATTTTAC+TGG + chr8_1:74739799-74739818 Msa1214240:three_prime_UTR 15.0%
!!! ATATAGTGGAATATATTTTT+CGG - chr8_1:74740074-74740093 None:intergenic 15.0%
!! TGTAATATTCTTTAATATAG+TGG - chr8_1:74740088-74740107 None:intergenic 15.0%
!! TAAAATAATAGCATTAGAAT+AGG - chr8_1:74740163-74740182 None:intergenic 15.0%
!! ATAGAATTAGAAATAGTCTT+TGG - chr8_1:74737156-74737175 None:intergenic 20.0%
!! TTTCTAATTCTATCATTGTA+CGG + chr8_1:74737163-74737182 Msa1214240:five_prime_UTR 20.0%
!!! AATTTAAATCTTGTTGTTTC+AGG + chr8_1:74737430-74737449 Msa1214240:intron 20.0%
!!! ATTTAAATCTTGTTGTTTCA+GGG + chr8_1:74737431-74737450 Msa1214240:intron 20.0%
!! TAAGCAATTTCTTTGAATAA+TGG - chr8_1:74737530-74737549 None:intergenic 20.0%
!! TAATCTCTTTAAATAAAGGT+AGG - chr8_1:74737872-74737891 None:intergenic 20.0%
!! CCTTTAATCTCTTTAAATAA+AGG - chr8_1:74737876-74737895 None:intergenic 20.0%
!!! CCTTTATTTAAAGAGATTAA+AGG + chr8_1:74737873-74737892 Msa1214240:intron 20.0%
!! CAAAAGAACATTGAATTATT+TGG + chr8_1:74738649-74738668 Msa1214240:intron 20.0%
!! AACATTGAATTATTTGGATA+TGG + chr8_1:74738655-74738674 Msa1214240:intron 20.0%
!! TATAAAACACAATCAAAAGA+CGG - chr8_1:74738774-74738793 None:intergenic 20.0%
!!! TCAGTACATTTTTACATTTA+TGG + chr8_1:74739052-74739071 Msa1214240:intron 20.0%
!!! GTATATATTTTTCATCATAC+TGG + chr8_1:74739379-74739398 Msa1214240:three_prime_UTR 20.0%
!! AAAAAACATTATCCATGTTA+AGG + chr8_1:74739715-74739734 Msa1214240:three_prime_UTR 20.0%
!! CAATTTCTTTGAATAATGGA+AGG - chr8_1:74737526-74737545 None:intergenic 25.0%
!!! TGATTATTTGTTTCTGTGAA+AGG + chr8_1:74737603-74737622 Msa1214240:intron 25.0%
!!! GATTATTTGTTTCTGTGAAA+GGG + chr8_1:74737604-74737623 Msa1214240:intron 25.0%
!! TTGTTGTTATTCGCTAATTA+TGG + chr8_1:74737768-74737787 Msa1214240:intron 25.0%
! AATTGTATTGTTTGATGTCT+TGG - chr8_1:74737951-74737970 None:intergenic 25.0%
!!! AGGTTTTAGATAAGAGATTA+AGG - chr8_1:74738088-74738107 None:intergenic 25.0%
! AAAAATTATACAACGGAACA+AGG - chr8_1:74738108-74738127 None:intergenic 25.0%
!! TAAAGTCTTCATTTTGTAGA+TGG + chr8_1:74738274-74738293 Msa1214240:intron 25.0%
!!! GTTTCTCATTTGATTTTTCT+TGG + chr8_1:74738316-74738335 Msa1214240:intron 25.0%
! CTTAATACATACAAACAAAC+AGG - chr8_1:74738586-74738605 None:intergenic 25.0%
!! TATATTACTGACTGTGTAAT+TGG - chr8_1:74738697-74738716 None:intergenic 25.0%
! TAATGTCTTTCGTTTCTATA+TGG + chr8_1:74738798-74738817 Msa1214240:intron 25.0%
!! CAAGATAAGATTTGTTTGAT+AGG + chr8_1:74738827-74738846 Msa1214240:intron 25.0%
!! GTTTCATTTCTCTTTTACTA+AGG - chr8_1:74738913-74738932 None:intergenic 25.0%
! AAATAATGTCAACAATTTCC+CGG + chr8_1:74738933-74738952 Msa1214240:intron 25.0%
! AAGGATTGACAACAATAAAA+TGG + chr8_1:74739177-74739196 Msa1214240:CDS 25.0%
!! CTACATGAATTAATTTTCAC+AGG + chr8_1:74739251-74739270 Msa1214240:intron 25.0%
! TTTATTCGTAGTCTATTAGT+TGG + chr8_1:74739428-74739447 Msa1214240:three_prime_UTR 25.0%
! TTCTGTCTTCTCAATTTAAT+TGG + chr8_1:74739489-74739508 Msa1214240:three_prime_UTR 25.0%
! TCTGTCTTCTCAATTTAATT+GGG + chr8_1:74739490-74739509 Msa1214240:three_prime_UTR 25.0%
! TCCAAAGTAAAATACATTAC+AGG + chr8_1:74739548-74739567 Msa1214240:three_prime_UTR 25.0%
!! ACCTGTAATGTATTTTACTT+TGG - chr8_1:74739552-74739571 None:intergenic 25.0%
! TAAACAACTATTCCTTAACA+TGG - chr8_1:74739730-74739749 None:intergenic 25.0%
!! ATAGTTGTTTACAGCTTTAT+TGG + chr8_1:74739739-74739758 Msa1214240:three_prime_UTR 25.0%
!! TATTGGTCTATTTGTTGAAT+TGG + chr8_1:74739756-74739775 Msa1214240:three_prime_UTR 25.0%
!! TTGAATTGGTTCTTAATTGA+TGG + chr8_1:74739770-74739789 Msa1214240:three_prime_UTR 25.0%
! ATCACTATATTGTATCTCTT+GGG - chr8_1:74740124-74740143 None:intergenic 25.0%
! AATCACTATATTGTATCTCT+TGG - chr8_1:74740125-74740144 None:intergenic 25.0%
!!! GCTATTATTTTATAGCATCT+AGG + chr8_1:74740171-74740190 Msa1214240:three_prime_UTR 25.0%
TAATTCTATCATTGTACGGT+TGG + chr8_1:74737167-74737186 Msa1214240:five_prime_UTR 30.0%
ATTAGCGAATAACAACAAGT+TGG - chr8_1:74737766-74737785 None:intergenic 30.0%
CATCAAAATCTATGCTATTG+CGG + chr8_1:74737831-74737850 Msa1214240:intron 30.0%
AAGAGATTAAAGGAAATGCA+CGG + chr8_1:74737883-74737902 Msa1214240:intron 30.0%
! AAGAAAGTGTACTTTCTTTC+AGG + chr8_1:74738132-74738151 Msa1214240:intron 30.0%
! AGAAAGTGTACTTTCTTTCA+GGG + chr8_1:74738133-74738152 Msa1214240:intron 30.0%
ATTGAGGAAAAAACTAGTTG+AGG + chr8_1:74738225-74738244 Msa1214240:CDS 30.0%
CAAACAAACAGGTATCAAAA+TGG - chr8_1:74738575-74738594 None:intergenic 30.0%
!! CTAGCAAATAATGGACATTT+AGG + chr8_1:74738743-74738762 Msa1214240:intron 30.0%
! GATAAGATTTGTTTGATAGG+AGG + chr8_1:74738830-74738849 Msa1214240:intron 30.0%
TTGATAGGAGGACTATATTT+AGG + chr8_1:74738842-74738861 Msa1214240:intron 30.0%
TGATAGGAGGACTATATTTA+GGG + chr8_1:74738843-74738862 Msa1214240:intron 30.0%
!! CCTTTTTGCTTTCATTTTTC+CGG - chr8_1:74739161-74739180 None:intergenic 30.0%
TCTGCTATATATGCTTCTAA+AGG + chr8_1:74739205-74739224 Msa1214240:CDS 30.0%
GAAATGTTCATTCAGAACTA+TGG - chr8_1:74739284-74739303 None:intergenic 30.0%
!! TAGTTCTGAATGAACATTTC+TGG + chr8_1:74739284-74739303 Msa1214240:intron 30.0%
CTTCAAATTTCGTATTAGAG+AGG - chr8_1:74739317-74739336 None:intergenic 30.0%
TAAAATACATTACAGGTCAC+AGG + chr8_1:74739555-74739574 Msa1214240:three_prime_UTR 30.0%
AAAATACATTACAGGTCACA+GGG + chr8_1:74739556-74739575 Msa1214240:three_prime_UTR 30.0%
ATCAAAGATTTAGCAATCCA+GGG - chr8_1:74739651-74739670 None:intergenic 30.0%
AACCACTCATTTCAAATGTA+TGG + chr8_1:74739839-74739858 Msa1214240:three_prime_UTR 30.0%
! TACCATACATTTGAAATGAG+TGG - chr8_1:74739844-74739863 None:intergenic 30.0%
CATTTCAAATGTATGGTACT+TGG + chr8_1:74739846-74739865 Msa1214240:three_prime_UTR 30.0%
GAAATTTCGGGAATCGAATT+TGG + chr8_1:74737193-74737212 Msa1214240:five_prime_UTR 35.0%
!! AGTAGAAATCGAAAGTATCG+AGG - chr8_1:74737331-74737350 None:intergenic 35.0%
AAGAAGAAGAAGAAGCGAAA+GGG - chr8_1:74737367-74737386 None:intergenic 35.0%
! TCAGAAGGTTCAATCTGTTT+TGG + chr8_1:74737642-74737661 Msa1214240:CDS 35.0%
!! GAACATACATTGAGAGTGTT+TGG - chr8_1:74737739-74737758 None:intergenic 35.0%
TTCGCTAATTATGGTCAATG+TGG + chr8_1:74737777-74737796 Msa1214240:intron 35.0%
ATGCTCAGTTAGAATCATTG+AGG + chr8_1:74738209-74738228 Msa1214240:CDS 35.0%
TAGTTGAGGTAATAAGAGTG+TGG + chr8_1:74738239-74738258 Msa1214240:intron 35.0%
! TTGGTAATAAATGTGCTGTC+AGG + chr8_1:74738335-74738354 Msa1214240:intron 35.0%
TAATAAATGTGCTGTCAGGT+GGG + chr8_1:74738339-74738358 Msa1214240:intron 35.0%
ATTTGAGCAAAACTCTTACG+CGG + chr8_1:74738452-74738471 Msa1214240:CDS 35.0%
GATAGGAGGACTATATTTAG+GGG + chr8_1:74738844-74738863 Msa1214240:intron 35.0%
ATAGGAGGACTATATTTAGG+GGG + chr8_1:74738845-74738864 Msa1214240:intron 35.0%
CTGACCTATGATACCTATAT+TGG - chr8_1:74738873-74738892 None:intergenic 35.0%
CAATATAGGTATCATAGGTC+AGG + chr8_1:74738871-74738890 Msa1214240:intron 35.0%
CAAGTAGGCAAGAATGTATT+TGG + chr8_1:74738971-74738990 Msa1214240:intron 35.0%
CCGGAAAAATGAAAGCAAAA+AGG + chr8_1:74739158-74739177 Msa1214240:CDS 35.0%
GACTACGAATAAACAATCAC+AGG - chr8_1:74739421-74739440 None:intergenic 35.0%
TTCTCAATTTAATTGGGCGA+AGG + chr8_1:74739496-74739515 Msa1214240:three_prime_UTR 35.0%
CATTGTTAGCTGAGTTTACT+AGG + chr8_1:74739614-74739633 Msa1214240:three_prime_UTR 35.0%
CATCAAAGATTTAGCAATCC+AGG - chr8_1:74739652-74739671 None:intergenic 35.0%
! CCTTTACATATTCCCCTTTT+GGG - chr8_1:74739684-74739703 None:intergenic 35.0%
CCAAAAGGGGAATATGTAAA+GGG + chr8_1:74739682-74739701 Msa1214240:three_prime_UTR 35.0%
AGCGAATCTCTACTTTCTAA+AGG - chr8_1:74739880-74739899 None:intergenic 35.0%
!!! AGATTCGCTTATTTTGTCGT+TGG + chr8_1:74739891-74739910 Msa1214240:three_prime_UTR 35.0%
!!! GATTCGCTTATTTTGTCGTT+GGG + chr8_1:74739892-74739911 Msa1214240:three_prime_UTR 35.0%
TTCCGTAAATAATACTGCAG+CGG + chr8_1:74739974-74739993 Msa1214240:three_prime_UTR 35.0%
TACCGCTGCAGTATTATTTA+CGG - chr8_1:74739979-74739998 None:intergenic 35.0%
! GTTTTTCCTTCGGTATTAGA+GGG - chr8_1:74740038-74740057 None:intergenic 35.0%
TAATACCGAAGGAAAAACCA+CGG + chr8_1:74740040-74740059 Msa1214240:three_prime_UTR 35.0%
GTACGGTTGGACTGAAATTT+CGG + chr8_1:74737180-74737199 Msa1214240:five_prime_UTR 40.0%
TACGGTTGGACTGAAATTTC+GGG + chr8_1:74737181-74737200 Msa1214240:five_prime_UTR 40.0%
ATTTCGGGAATCGAATTTGG+CGG + chr8_1:74737196-74737215 Msa1214240:five_prime_UTR 40.0%
ACTTCGTTGAAGAAGAGTTG+TGG - chr8_1:74737284-74737303 None:intergenic 40.0%
AGAAGAAGAAGAAGCGAAAG+GGG - chr8_1:74737366-74737385 None:intergenic 40.0%
GAAGAAGAAGAAGAAGCGAA+AGG - chr8_1:74737368-74737387 None:intergenic 40.0%
TGAAACCTGTGTGAGATGTT+GGG - chr8_1:74737496-74737515 None:intergenic 40.0%
ATGAAACCTGTGTGAGATGT+TGG - chr8_1:74737497-74737516 None:intergenic 40.0%
! TGTTGATTGCATTCGTCAGA+AGG + chr8_1:74737627-74737646 Msa1214240:CDS 40.0%
!! CATTGCTTTTCTCTTCCTGA+CGG + chr8_1:74737703-74737722 Msa1214240:CDS 40.0%
TTTGGCAATTGAAAACCGTC+AGG - chr8_1:74737721-74737740 None:intergenic 40.0%
ATGTCTTGGCAAGTTGTGAT+TGG - chr8_1:74737937-74737956 None:intergenic 40.0%
TTCTTTCAGGGTGAAACAAG+TGG + chr8_1:74738145-74738164 Msa1214240:intron 40.0%
TCTTTCAGGGTGAAACAAGT+GGG + chr8_1:74738146-74738165 Msa1214240:intron 40.0%
CTGAGCATCTAATTCCAGAT+TGG - chr8_1:74738197-74738216 None:intergenic 40.0%
GTAATAAATGTGCTGTCAGG+TGG + chr8_1:74738338-74738357 Msa1214240:intron 40.0%
AATAAATGTGCTGTCAGGTG+GGG + chr8_1:74738340-74738359 Msa1214240:intron 40.0%
!! AATACCAGTTCTGAGTAGCA+CGG - chr8_1:74738547-74738566 None:intergenic 40.0%
!! TATTTAGGGGGCACCAATAT+AGG + chr8_1:74738857-74738876 Msa1214240:intron 40.0%
GGCACCAATATAGGTATCAT+AGG + chr8_1:74738866-74738885 Msa1214240:intron 40.0%
AGCTCTGCATTATTGCAAGT+AGG + chr8_1:74738956-74738975 Msa1214240:intron 40.0%
TTGTGTTCCTGCAGAGATTA+TGG + chr8_1:74739075-74739094 Msa1214240:intron 40.0%
TATCTTGAGTCGAAGTTCTG+AGG - chr8_1:74739107-74739126 None:intergenic 40.0%
TCAGAACTTCGACTCAAGAT+AGG + chr8_1:74739106-74739125 Msa1214240:CDS 40.0%
ACCAACAAGATTGAAGAGAC+CGG + chr8_1:74739139-74739158 Msa1214240:CDS 40.0%
!! GAATGAACATTTCTGGTTGC+AGG + chr8_1:74739291-74739310 Msa1214240:intron 40.0%
TGATGAGATTTCTGCCCAAA+AGG + chr8_1:74739667-74739686 Msa1214240:three_prime_UTR 40.0%
GATGAGATTTCTGCCCAAAA+GGG + chr8_1:74739668-74739687 Msa1214240:three_prime_UTR 40.0%
ATGAGATTTCTGCCCAAAAG+GGG + chr8_1:74739669-74739688 Msa1214240:three_prime_UTR 40.0%
CCCAAAAGGGGAATATGTAA+AGG + chr8_1:74739681-74739700 Msa1214240:three_prime_UTR 40.0%
! CCCTTTACATATTCCCCTTT+TGG - chr8_1:74739685-74739704 None:intergenic 40.0%
TGGAACACCAAGATTTAACG+TGG + chr8_1:74740008-74740027 Msa1214240:three_prime_UTR 40.0%
! GGGTTTTCCACGTTAAATCT+TGG - chr8_1:74740018-74740037 None:intergenic 40.0%
! GGTTTTTCCTTCGGTATTAG+AGG - chr8_1:74740039-74740058 None:intergenic 40.0%
AATACCGAAGGAAAAACCAC+GGG + chr8_1:74740041-74740060 Msa1214240:three_prime_UTR 40.0%
TTATAGAGAAGATACCCACC+CGG + chr8_1:74740196-74740215 Msa1214240:three_prime_UTR 40.0%
AGAGTGTGGGAAAAATACAC+CGG - chr8_1:74740218-74740237 None:intergenic 40.0%
! TGTATTTTTCCCACACTCTC+TGG + chr8_1:74740219-74740238 Msa1214240:three_prime_UTR 40.0%
! TGAGTAAATCCAGAGAGTGT+GGG - chr8_1:74740231-74740250 None:intergenic 40.0%
TTGAGTAAATCCAGAGAGTG+TGG - chr8_1:74740232-74740251 None:intergenic 40.0%
TTTCGGGAATCGAATTTGGC+GGG + chr8_1:74737197-74737216 Msa1214240:five_prime_UTR 45.0%
!! AAGCGAAAGGGGTTAGTTAC+TGG - chr8_1:74737355-74737374 None:intergenic 45.0%
AAGATCCCAACATCTCACAC+AGG + chr8_1:74737488-74737507 Msa1214240:CDS 45.0%
TGGATCAAAAGCTCGCTGTT+TGG + chr8_1:74737662-74737681 Msa1214240:CDS 45.0%
GGATCAAAAGCTCGCTGTTT+GGG + chr8_1:74737663-74737682 Msa1214240:CDS 45.0%
!! ACTGCAATGGACTGTGATTG+AGG - chr8_1:74737803-74737822 None:intergenic 45.0%
TGATGTCTCTGTGACTGCAA+TGG - chr8_1:74737816-74737835 None:intergenic 45.0%
AAGGTAGGCGACCACAATAT+GGG - chr8_1:74737857-74737876 None:intergenic 45.0%
AAAGGTAGGCGACCACAATA+TGG - chr8_1:74737858-74737877 None:intergenic 45.0%
AGTGGGAATGTCATTGATCC+TGG + chr8_1:74738163-74738182 Msa1214240:CDS 45.0%
!! TCCTGGTTCTACTTCCAATC+TGG + chr8_1:74738180-74738199 Msa1214240:CDS 45.0%
TCCAGATTGGAAGTAGAACC+AGG - chr8_1:74738184-74738203 None:intergenic 45.0%
GTCAGTCTTCTCACAATGCT+CGG + chr8_1:74738406-74738425 Msa1214240:CDS 45.0%
CAATGCTCGGTATATCAACG+AGG + chr8_1:74738419-74738438 Msa1214240:CDS 45.0%
! TCTTACGCGGAGTTGTTTCA+GGG + chr8_1:74738465-74738484 Msa1214240:CDS 45.0%
!! ATACCAGTTCTGAGTAGCAC+GGG - chr8_1:74738546-74738565 None:intergenic 45.0%
TTGCAATAATGCAGAGCTCC+GGG - chr8_1:74738954-74738973 None:intergenic 45.0%
CTTGCAATAATGCAGAGCTC+CGG - chr8_1:74738955-74738974 None:intergenic 45.0%
TCCGGTCTCTTCAATCTTGT+TGG - chr8_1:74739143-74739162 None:intergenic 45.0%
CCACTCATTTCAGCATGAGA+AGG - chr8_1:74739459-74739478 None:intergenic 45.0%
CCTTCTCATGCTGAAATGAG+TGG + chr8_1:74739456-74739475 Msa1214240:three_prime_UTR 45.0%
ACTAGGTTGTGATAAAGCCC+TGG + chr8_1:74739631-74739650 Msa1214240:three_prime_UTR 45.0%
! TATTTTGTCGTTGGGCTCGA+CGG + chr8_1:74739900-74739919 Msa1214240:three_prime_UTR 45.0%
CTGCAGCGGTAGCAATAAAA+TGG + chr8_1:74739988-74740007 Msa1214240:three_prime_UTR 45.0%
GGAAAACCCTCTAATACCGA+AGG + chr8_1:74740029-74740048 Msa1214240:three_prime_UTR 45.0%
! TTGTCCCGTGGTTTTTCCTT+CGG - chr8_1:74740048-74740067 None:intergenic 45.0%
!! ATTTTTCGGAGTTTGTCCCG+TGG - chr8_1:74740060-74740079 None:intergenic 45.0%
AGAATAGGAGTGAGTCTCAC+AGG - chr8_1:74740148-74740167 None:intergenic 45.0%
GAGTGTGGGAAAAATACACC+GGG - chr8_1:74740217-74740236 None:intergenic 45.0%
!! AATTTAATTAACTTTAATTA+GGG + chr8_1:74737554-74737573 Msa1214240:intron 5.0%
GGAATCGAATTTGGCGGGAA+TGG + chr8_1:74737202-74737221 Msa1214240:exon 50.0%
TCGAATTTGGCGGGAATGGA+AGG + chr8_1:74737206-74737225 Msa1214240:exon 50.0%
!! GAGCGACGCTGTTTTCGAAT+CGG + chr8_1:74737244-74737263 Msa1214240:CDS 50.0%
AGCATCCACCAAGCTCAATG+CGG + chr8_1:74737455-74737474 Msa1214240:CDS 50.0%
AGTAGCTTCCGCATTGAGCT+TGG - chr8_1:74737466-74737485 None:intergenic 50.0%
TGCTATTGCGGCCCATATTG+TGG + chr8_1:74737843-74737862 Msa1214240:intron 50.0%
! CTCTTACGCGGAGTTGTTTC+AGG + chr8_1:74738464-74738483 Msa1214240:CDS 50.0%
!! CTTGAGTCGAAGTTCTGAGG+CGG - chr8_1:74739104-74739123 None:intergenic 50.0%
! ATTTTGTCGTTGGGCTCGAC+GGG + chr8_1:74739901-74739920 Msa1214240:three_prime_UTR 50.0%
GTGGGAAAAATACACCGGGT+GGG - chr8_1:74740213-74740232 None:intergenic 50.0%
TGTGGGAAAAATACACCGGG+TGG - chr8_1:74740214-74740233 None:intergenic 50.0%
AGCTTCCGCATTGAGCTTGG+TGG - chr8_1:74737463-74737482 None:intergenic 55.0%
! GATCCCGTGCTACTCAGAAC+TGG + chr8_1:74738540-74738559 Msa1214240:intron 55.0%
GCGGTTGCCATAATCTCTGC+AGG - chr8_1:74739085-74739104 None:intergenic 55.0%
TGGGCGAAGGCAGAAGCTAG+AGG + chr8_1:74739509-74739528 Msa1214240:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 74737151 74740256 74737151 ID=Msa1214240;Name=Msa1214240
chr8_1 mRNA 74737151 74740256 74737151 ID=Msa1214240-mRNA-1;Parent=Msa1214240;Name=Msa1214240-mRNA-1;_AED=0.04;_eAED=0.04;_QI=70|1|1|1|1|1|7|882|246
chr8_1 exon 74737151 74737354 74737151 ID=Msa1214240-mRNA-1:exon:25615;Parent=Msa1214240-mRNA-1
chr8_1 exon 74737452 74737509 74737452 ID=Msa1214240-mRNA-1:exon:25616;Parent=Msa1214240-mRNA-1
chr8_1 exon 74737625 74737750 74737625 ID=Msa1214240-mRNA-1:exon:25617;Parent=Msa1214240-mRNA-1
chr8_1 exon 74738154 74738246 74738154 ID=Msa1214240-mRNA-1:exon:25618;Parent=Msa1214240-mRNA-1
chr8_1 exon 74738357 74738486 74738357 ID=Msa1214240-mRNA-1:exon:25619;Parent=Msa1214240-mRNA-1
chr8_1 exon 74739089 74739226 74739089 ID=Msa1214240-mRNA-1:exon:25620;Parent=Msa1214240-mRNA-1
chr8_1 exon 74739313 74740256 74739313 ID=Msa1214240-mRNA-1:exon:25621;Parent=Msa1214240-mRNA-1
chr8_1 five_prime_UTR 74737151 74737220 74737151 ID=Msa1214240-mRNA-1:five_prime_utr;Parent=Msa1214240-mRNA-1
chr8_1 CDS 74737221 74737354 74737221 ID=Msa1214240-mRNA-1:cds;Parent=Msa1214240-mRNA-1
chr8_1 CDS 74737452 74737509 74737452 ID=Msa1214240-mRNA-1:cds;Parent=Msa1214240-mRNA-1
chr8_1 CDS 74737625 74737750 74737625 ID=Msa1214240-mRNA-1:cds;Parent=Msa1214240-mRNA-1
chr8_1 CDS 74738154 74738246 74738154 ID=Msa1214240-mRNA-1:cds;Parent=Msa1214240-mRNA-1
chr8_1 CDS 74738357 74738486 74738357 ID=Msa1214240-mRNA-1:cds;Parent=Msa1214240-mRNA-1
chr8_1 CDS 74739089 74739226 74739089 ID=Msa1214240-mRNA-1:cds;Parent=Msa1214240-mRNA-1
chr8_1 CDS 74739313 74739374 74739313 ID=Msa1214240-mRNA-1:cds;Parent=Msa1214240-mRNA-1
chr8_1 three_prime_UTR 74739375 74740256 74739375 ID=Msa1214240-mRNA-1:three_prime_utr;Parent=Msa1214240-mRNA-1
Gene Sequence

>Msa1214240

ATGGAAGGAAGCATGAGCAGAGAGAGCGACGCTGTTTTCGAATCGGTGATGAATCTGAATCCACAACTCTTCTTCAACGAAGTTCTCAACACTGTTGACGATTTCGTCCTCGATACTTTCGATTTCTACTTCCAGGAAGCATCCACCAAGCTCAATGCGGAAGCTACTCAAAGATCCCAACATCTCACACAGGGTGTTGATTGCATTCGTCAGAAGGTTCAATCTGTTTTGGATCAAAAGCTCGCTGTTTGGGAAAAATACTGTCTTTATCATTGCTTTTCTCTTCCTGACGGTTTTCAATTGCCAAACACTCTCAATGGTGAAACAAGTGGGAATGTCATTGATCCTGGTTCTACTTCCAATCTGGAATTAGATGCTCAGTTAGAATCATTGAGGAAAAAACTAGTTGAGGTGGGGAAAGAGTCTGAAATGCTGAATCAAGAAATTCATGCATTAGAGAGTCAGTCTTCTCACAATGCTCGGTATATCAACGAGGCAGTACAATTATTTGAGCAAAACTCTTACGCGGAGTTGTTTCAGGAGATTATGGCAACCGCCTCAGAACTTCGACTCAAGATAGGAAAGCTAAACACCAACAAGATTGAAGAGACCGGAAAAATGAAAGCAAAAAGGATTGACAACAATAAAATGGACATTTCTGCTATATATGCTTCTAAAGGCCTCTCTAATACGAAATTTGAAGATCTTCAAGAATTTGTAACTCTTATGAAGAGTACTTGA
Protein sequence

>Msa1214240

MEGSMSRESDAVFESVMNLNPQLFFNEVLNTVDDFVLDTFDFYFQEASTKLNAEATQRSQHLTQGVDCIRQKVQSVLDQKLAVWEKYCLYHCFSLPDGFQLPNTLNGETSGNVIDPGSTSNLELDAQLESLRKKLVEVGKESEMLNQEIHALESQSSHNARYINEAVQLFEQNSYAELFQEIMATASELRLKIGKLNTNKIEETGKMKAKRIDNNKMDISAIYASKGLSNTKFEDLQEFVTLMKST*