Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008274.t1 | XP_003600461.1 | 93.6 | 313 | 6 | 3 | 1 | 299 | 1 | 313 | 1.30E-169 | 605.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008274.t1 | Q9FKT5 | 80.2 | 313 | 47 | 4 | 2 | 299 | 3 | 315 | 4.6e-147 | 521.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008274.t1 | G7IY98 | 93.6 | 313 | 6 | 3 | 1 | 299 | 1 | 313 | 9.5e-170 | 605.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene004825 | MS.gene008274 | PPI |
| MS.gene008274 | MS.gene008794 | PPI |
| MS.gene008793 | MS.gene008274 | PPI |
| MS.gene008274 | MS.gene014281 | PPI |
| MS.gene52980 | MS.gene008274 | PPI |
| MS.gene008274 | MS.gene93058 | PPI |
| MS.gene042253 | MS.gene008274 | PPI |
| MS.gene046235 | MS.gene008274 | PPI |
| MS.gene008274 | MS.gene012608 | PPI |
| MS.gene056488 | MS.gene008274 | PPI |
| MS.gene68222 | MS.gene008274 | PPI |
| MS.gene93059 | MS.gene008274 | PPI |
| MS.gene008274 | MS.gene71431 | PPI |
| MS.gene95244 | MS.gene008274 | PPI |
| MS.gene97981 | MS.gene008274 | PPI |
| MS.gene064894 | MS.gene008274 | PPI |
| MS.gene33535 | MS.gene008274 | PPI |
| MS.gene008274 | MS.gene97980 | PPI |
| MS.gene008274 | MS.gene063165 | PPI |
| MS.gene066026 | MS.gene008274 | PPI |
| MS.gene056487 | MS.gene008274 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008274.t1 | MTR_3g061450 | 93.610 | 313 | 6 | 3 | 1 | 299 | 1 | 313 | 0.0 | 601 |
| MS.gene008274.t1 | MTR_4g095058 | 27.397 | 292 | 177 | 11 | 11 | 275 | 278 | 561 | 1.75e-15 | 77.0 |
| MS.gene008274.t1 | MTR_4g095058 | 30.357 | 168 | 91 | 7 | 8 | 158 | 400 | 558 | 7.47e-13 | 68.9 |
| MS.gene008274.t1 | MTR_4g095058 | 27.397 | 292 | 177 | 11 | 11 | 275 | 280 | 563 | 1.77e-15 | 77.0 |
| MS.gene008274.t1 | MTR_4g095058 | 30.357 | 168 | 91 | 7 | 8 | 158 | 402 | 560 | 8.28e-13 | 68.9 |
| MS.gene008274.t1 | MTR_3g106080 | 26.316 | 247 | 126 | 6 | 15 | 244 | 457 | 664 | 2.83e-15 | 76.6 |
| MS.gene008274.t1 | MTR_4g125710 | 24.254 | 268 | 170 | 7 | 13 | 251 | 54 | 317 | 1.58e-13 | 70.1 |
| MS.gene008274.t1 | MTR_5g016370 | 29.560 | 159 | 94 | 5 | 13 | 158 | 424 | 577 | 1.98e-13 | 70.9 |
| MS.gene008274.t1 | MTR_5g016370 | 23.867 | 331 | 193 | 11 | 17 | 293 | 245 | 570 | 5.02e-12 | 66.6 |
| MS.gene008274.t1 | MTR_5g016350 | 23.708 | 329 | 198 | 9 | 17 | 293 | 231 | 558 | 2.02e-13 | 70.9 |
| MS.gene008274.t1 | MTR_5g016350 | 30.435 | 161 | 90 | 6 | 13 | 158 | 412 | 565 | 7.72e-13 | 68.9 |
| MS.gene008274.t1 | MTR_5g016350 | 23.708 | 329 | 198 | 9 | 17 | 293 | 167 | 494 | 2.86e-13 | 70.1 |
| MS.gene008274.t1 | MTR_5g016350 | 30.435 | 161 | 90 | 6 | 13 | 158 | 348 | 501 | 1.53e-12 | 68.2 |
| MS.gene008274.t1 | MTR_3g449990 | 25.263 | 285 | 191 | 9 | 17 | 281 | 268 | 550 | 2.74e-12 | 67.4 |
| MS.gene008274.t1 | MTR_4g119620 | 25.101 | 247 | 163 | 7 | 13 | 244 | 72 | 311 | 3.97e-12 | 66.2 |
| MS.gene008274.t1 | MTR_1g097140 | 27.016 | 248 | 158 | 9 | 12 | 246 | 116 | 353 | 2.61e-11 | 63.9 |
| MS.gene008274.t1 | MTR_7g086360 | 22.373 | 295 | 198 | 10 | 16 | 284 | 52 | 341 | 5.82e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008274.t1 | AT5G56130 | 80.317 | 315 | 46 | 5 | 1 | 299 | 1 | 315 | 0.0 | 518 |
| MS.gene008274.t1 | AT5G25150 | 25.301 | 249 | 126 | 7 | 15 | 244 | 456 | 663 | 4.21e-14 | 73.2 |
| MS.gene008274.t1 | AT5G25150 | 27.228 | 202 | 103 | 6 | 15 | 213 | 456 | 616 | 5.18e-13 | 69.7 |
| MS.gene008274.t1 | AT2G43770 | 24.746 | 295 | 191 | 11 | 16 | 284 | 50 | 339 | 6.74e-13 | 68.6 |
| MS.gene008274.t1 | AT5G67320 | 29.747 | 158 | 95 | 4 | 12 | 157 | 441 | 594 | 9.00e-13 | 68.9 |
| MS.gene008274.t1 | AT5G67320 | 24.921 | 317 | 188 | 9 | 9 | 282 | 255 | 564 | 6.35e-12 | 66.2 |
| MS.gene008274.t1 | AT5G23430 | 24.000 | 275 | 149 | 9 | 13 | 269 | 5 | 237 | 1.42e-11 | 65.5 |
| MS.gene008274.t1 | AT5G23430 | 24.000 | 275 | 149 | 9 | 13 | 269 | 5 | 237 | 1.42e-11 | 65.5 |
| MS.gene008274.t1 | AT5G23430 | 24.000 | 275 | 149 | 9 | 13 | 269 | 5 | 237 | 1.43e-11 | 65.5 |
| MS.gene008274.t1 | AT5G08390 | 25.410 | 244 | 130 | 8 | 13 | 246 | 5 | 206 | 2.19e-11 | 65.1 |
Find 72 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGCAAGTACATTGTATTT+AGG | 0.212077 | 3.1:+54929587 | None:intergenic |
| TTGATCAAACTGCTCGAATT+TGG | 0.221602 | 3.1:-54936579 | MS.gene008274:CDS |
| ACAATTAGTTTCAATCATAC+TGG | 0.283022 | 3.1:-54930221 | MS.gene008274:CDS |
| TGCAATAAGATCGGGATGTT+TGG | 0.289165 | 3.1:+54935494 | None:intergenic |
| GCAATAAGATCGGGATGTTT+GGG | 0.297004 | 3.1:+54935495 | None:intergenic |
| GTGACAAGACTGTTCGTCTA+TGG | 0.297252 | 3.1:-54935461 | MS.gene008274:CDS |
| CGATCTTATTGCAACTGCTT+CGG | 0.300112 | 3.1:-54935484 | MS.gene008274:CDS |
| CTCATGCAGCTGTAGGTAAT+AGG | 0.305067 | 3.1:-54935180 | MS.gene008274:CDS |
| TGTCAGCTTGTGGGTTATTT+CGG | 0.322981 | 3.1:-54930400 | MS.gene008274:CDS |
| GATCTTATTGCAACTGCTTC+GGG | 0.329060 | 3.1:-54935483 | MS.gene008274:CDS |
| CTGATTCCCTTGTCAGCTTG+TGG | 0.378858 | 3.1:-54930410 | MS.gene008274:CDS |
| TTCTTTCTAACAACAGGAAA+TGG | 0.391405 | 3.1:-54930814 | MS.gene008274:intron |
| GCTCAATAAATGAGATTGCT+TGG | 0.402499 | 3.1:-54930855 | MS.gene008274:intron |
| TGATTCCCTTGTCAGCTTGT+GGG | 0.409663 | 3.1:-54930409 | MS.gene008274:CDS |
| CAGTACATCATATTCCTTGT+AGG | 0.413376 | 3.1:-54929637 | MS.gene008274:CDS |
| CTTCACAGCAGAGAGTATTC+TGG | 0.419755 | 3.1:-54936821 | MS.gene008274:CDS |
| ATTGGCACAAAGCTTGCTTC+TGG | 0.427408 | 3.1:-54936607 | MS.gene008274:CDS |
| TGCATTGCAATTGACCCAAC+AGG | 0.430132 | 3.1:-54930607 | MS.gene008274:CDS |
| AAAAGGTGCATTCAGTGGCT+TGG | 0.441542 | 3.1:-54936636 | MS.gene008274:intron |
| GGTCAAATATATTGAATTGA+AGG | 0.444956 | 3.1:-54935553 | MS.gene008274:CDS |
| AGTTAACAATACTTGATGTT+CGG | 0.447811 | 3.1:-54934818 | MS.gene008274:CDS |
| GAGATGTTCTTTCTAACAAC+AGG | 0.457171 | 3.1:-54930820 | MS.gene008274:CDS |
| ATGAGTACCATCAGGTTTGT+AGG | 0.457397 | 3.1:+54935198 | None:intergenic |
| ATTCCACTCAACACTATTGA+TGG | 0.466131 | 3.1:+54929610 | None:intergenic |
| CAATTAGTTTCAATCATACT+GGG | 0.469646 | 3.1:-54930220 | MS.gene008274:CDS |
| TATGATTGAAACTAATTGTC+CGG | 0.470565 | 3.1:+54930225 | None:intergenic |
| TGTTTCTCAGATGGCCTGTC+CGG | 0.480041 | 3.1:-54930244 | MS.gene008274:intron |
| CTATTGATGGCAGCCCTACA+AGG | 0.486597 | 3.1:+54929623 | None:intergenic |
| AGGTGGAAAATGCTCACAAC+AGG | 0.499837 | 3.1:-54935251 | MS.gene008274:CDS |
| CGAAGCAGTTGCAATAAGAT+CGG | 0.502914 | 3.1:+54935485 | None:intergenic |
| TCATGCAGCTGTAGGTAATA+GGG | 0.503464 | 3.1:-54935179 | MS.gene008274:intron |
| CACAACAGGCAGAACTTAGT+GGG | 0.503581 | 3.1:-54935237 | MS.gene008274:CDS |
| GTATCAAGAGGTCGAAGAGA+TGG | 0.505442 | 3.1:+54930658 | None:intergenic |
| TAGTGTGGATCAGCTATGTT+GGG | 0.512894 | 3.1:-54935520 | MS.gene008274:CDS |
| TGACAAGACTGTTCGTCTAT+GGG | 0.513043 | 3.1:-54935460 | MS.gene008274:CDS |
| AACTTACTCGAGCTTTGTAA+AGG | 0.513452 | 3.1:+54930359 | None:intergenic |
| GTCAAATATATTGAATTGAA+GGG | 0.518728 | 3.1:-54935552 | MS.gene008274:CDS |
| AAGAAGCATACCTTCCTGTT+GGG | 0.521567 | 3.1:+54930593 | None:intergenic |
| TCAGTGGCTTGGAATTGCAT+TGG | 0.521845 | 3.1:-54936625 | MS.gene008274:CDS |
| TCACAACAGGCAGAACTTAG+TGG | 0.530916 | 3.1:-54935238 | MS.gene008274:CDS |
| AAACGAGAATTGTTCCTCCA+TGG | 0.533256 | 3.1:+54936850 | None:intergenic |
| GAAGCAGTTGCAATAAGATC+GGG | 0.535149 | 3.1:+54935486 | None:intergenic |
| TTGAACTTACGCCTATGCAT+TGG | 0.535702 | 3.1:+54934786 | None:intergenic |
| TACAATGTACTTGCATATGC+CGG | 0.539937 | 3.1:-54929581 | MS.gene008274:CDS |
| AGTACATCATATTCCTTGTA+GGG | 0.547208 | 3.1:-54929636 | MS.gene008274:CDS |
| CAAGAAGCATACCTTCCTGT+TGG | 0.550714 | 3.1:+54930592 | None:intergenic |
| TACTCTCATGGCTCATACAG+CGG | 0.555050 | 3.1:-54930638 | MS.gene008274:CDS |
| ACTCTCATGGCTCATACAGC+GGG | 0.555763 | 3.1:-54930637 | MS.gene008274:CDS |
| ATAGTGTGGATCAGCTATGT+TGG | 0.561550 | 3.1:-54935521 | MS.gene008274:CDS |
| AAATAACCCACAAGCTGACA+AGG | 0.562424 | 3.1:+54930403 | None:intergenic |
| TCGACCTCTTGATACTCTCA+TGG | 0.565472 | 3.1:-54930650 | MS.gene008274:CDS |
| TAGGCGTAAGTTCAACTATG+AGG | 0.584012 | 3.1:-54934778 | MS.gene008274:intron |
| GGAAATTCAAACCAATGCAT+AGG | 0.586683 | 3.1:-54934797 | MS.gene008274:CDS |
| ACAGCTGCATGAGTACCATC+AGG | 0.587950 | 3.1:+54935190 | None:intergenic |
| TTGAAACTAATTGTCCGGAC+AGG | 0.589710 | 3.1:+54930230 | None:intergenic |
| AAGGTTACTACATGGGCATG+TGG | 0.595342 | 3.1:+54936547 | None:intergenic |
| GTTTCAAAAGGTGCATTCAG+TGG | 0.599434 | 3.1:-54936641 | MS.gene008274:intron |
| TTTCGGAGATGCTTTGTGTG+CGG | 0.599990 | 3.1:-54930383 | MS.gene008274:CDS |
| GAGATTGCTTGGAACATGAC+TGG | 0.603670 | 3.1:-54930844 | MS.gene008274:CDS |
| GAAGGGTCACACCGATAGTG+TGG | 0.604201 | 3.1:-54935535 | MS.gene008274:CDS |
| AACATCACCTACAAACCTGA+TGG | 0.607731 | 3.1:-54935205 | MS.gene008274:CDS |
| GATGGTACTCATGCAGCTGT+AGG | 0.608815 | 3.1:-54935187 | MS.gene008274:CDS |
| AGGTTACTACATGGGCATGT+GGG | 0.614543 | 3.1:+54936548 | None:intergenic |
| CAATAAGATCGGGATGTTTG+GGG | 0.614895 | 3.1:+54935496 | None:intergenic |
| CTGCCATCAATAGTGTTGAG+TGG | 0.622139 | 3.1:-54929613 | MS.gene008274:CDS |
| ACATAGCTGATCCACACTAT+CGG | 0.628331 | 3.1:+54935524 | None:intergenic |
| AACAAGTATCAGGCTGACGA+AGG | 0.633197 | 3.1:-54929548 | MS.gene008274:intron |
| TTGCAATTGACCCAACAGGA+AGG | 0.660962 | 3.1:-54930603 | MS.gene008274:intron |
| AATAACCCACAAGCTGACAA+GGG | 0.681570 | 3.1:+54930404 | None:intergenic |
| AATTAGTTTCAATCATACTG+GGG | 0.695286 | 3.1:-54930219 | MS.gene008274:CDS |
| TGAGCCATGAGAGTATCAAG+AGG | 0.696186 | 3.1:+54930646 | None:intergenic |
| ACAACAGGCAGAACTTAGTG+GGG | 0.714393 | 3.1:-54935236 | MS.gene008274:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 54929427 | 54936870 | 54929427 | ID=MS.gene008274 |
| chr3.1 | mRNA | 54929427 | 54936870 | 54929427 | ID=MS.gene008274.t1;Parent=MS.gene008274 |
| chr3.1 | exon | 54936808 | 54936870 | 54936808 | ID=MS.gene008274.t1.exon1;Parent=MS.gene008274.t1 |
| chr3.1 | CDS | 54936808 | 54936870 | 54936808 | ID=cds.MS.gene008274.t1;Parent=MS.gene008274.t1 |
| chr3.1 | exon | 54936558 | 54936653 | 54936558 | ID=MS.gene008274.t1.exon2;Parent=MS.gene008274.t1 |
| chr3.1 | CDS | 54936558 | 54936653 | 54936558 | ID=cds.MS.gene008274.t1;Parent=MS.gene008274.t1 |
| chr3.1 | exon | 54935451 | 54935580 | 54935451 | ID=MS.gene008274.t1.exon3;Parent=MS.gene008274.t1 |
| chr3.1 | CDS | 54935451 | 54935580 | 54935451 | ID=cds.MS.gene008274.t1;Parent=MS.gene008274.t1 |
| chr3.1 | exon | 54935180 | 54935271 | 54935180 | ID=MS.gene008274.t1.exon4;Parent=MS.gene008274.t1 |
| chr3.1 | CDS | 54935180 | 54935271 | 54935180 | ID=cds.MS.gene008274.t1;Parent=MS.gene008274.t1 |
| chr3.1 | exon | 54934779 | 54934847 | 54934779 | ID=MS.gene008274.t1.exon5;Parent=MS.gene008274.t1 |
| chr3.1 | CDS | 54934779 | 54934847 | 54934779 | ID=cds.MS.gene008274.t1;Parent=MS.gene008274.t1 |
| chr3.1 | exon | 54930815 | 54930863 | 54930815 | ID=MS.gene008274.t1.exon6;Parent=MS.gene008274.t1 |
| chr3.1 | CDS | 54930815 | 54930863 | 54930815 | ID=cds.MS.gene008274.t1;Parent=MS.gene008274.t1 |
| chr3.1 | exon | 54930604 | 54930691 | 54930604 | ID=MS.gene008274.t1.exon7;Parent=MS.gene008274.t1 |
| chr3.1 | CDS | 54930604 | 54930691 | 54930604 | ID=cds.MS.gene008274.t1;Parent=MS.gene008274.t1 |
| chr3.1 | exon | 54930366 | 54930452 | 54930366 | ID=MS.gene008274.t1.exon8;Parent=MS.gene008274.t1 |
| chr3.1 | CDS | 54930366 | 54930452 | 54930366 | ID=cds.MS.gene008274.t1;Parent=MS.gene008274.t1 |
| chr3.1 | exon | 54930174 | 54930256 | 54930174 | ID=MS.gene008274.t1.exon9;Parent=MS.gene008274.t1 |
| chr3.1 | CDS | 54930174 | 54930256 | 54930174 | ID=cds.MS.gene008274.t1;Parent=MS.gene008274.t1 |
| chr3.1 | exon | 54929549 | 54929656 | 54929549 | ID=MS.gene008274.t1.exon10;Parent=MS.gene008274.t1 |
| chr3.1 | CDS | 54929549 | 54929656 | 54929549 | ID=cds.MS.gene008274.t1;Parent=MS.gene008274.t1 |
| chr3.1 | exon | 54929427 | 54929461 | 54929427 | ID=MS.gene008274.t1.exon11;Parent=MS.gene008274.t1 |
| chr3.1 | CDS | 54929427 | 54929461 | 54929427 | ID=cds.MS.gene008274.t1;Parent=MS.gene008274.t1 |
| Gene Sequence |
| Protein sequence |
