AlfalfaGEDB | MS.gene01270

M. sativa cultivar XinJiangDaYe / MS.gene01270

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01270.t1	AES63607.2	94.9	157	8	0	6	162	8	164	1.50E-87	332

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01270.t1	P11670	58.1	155	63	1	8	162	7	159	5.2e-52	205.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01270.t1	G7IFC9	94.9	157	8	0	6	162	8	164	1.1e-87	332.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene002294	MS.gene01270	PPI
MS.gene01687	MS.gene01270	PPI
MS.gene02252	MS.gene01270	PPI
MS.gene01690	MS.gene01270	PPI
MS.gene01685	MS.gene01270	PPI
MS.gene01275	MS.gene01270	PPI
MS.gene01270	MS.gene002296	PPI
MS.gene01270	MS.gene002297	PPI
MS.gene01277	MS.gene01270	PPI
MS.gene02255	MS.gene01270	PPI
MS.gene01270	MS.gene01274	PPI
MS.gene02256	MS.gene01270	PPI
MS.gene02254	MS.gene01270	PPI
MS.gene01273	MS.gene01270	PPI
MS.gene002299	MS.gene01270	PPI
MS.gene01686	MS.gene01270	PPI
MS.gene002295	MS.gene01270	PPI
MS.gene01272	MS.gene01270	PPI
MS.gene01688	MS.gene01270	PPI
MS.gene01270	MS.gene02258	PPI
MS.gene01356	MS.gene01270	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01270.t1	MTR_2g010580	94.904	157	8	0	6	162	8	164	7.64e-113	317
MS.gene01270.t1	MTR_4g092540	56.688	157	62	3	7	162	8	159	1.27e-62	190
MS.gene01270.t1	MTR_2g010590	50.000	156	72	2	8	162	9	159	9.04e-57	175
MS.gene01270.t1	MTR_2g435490	51.220	164	78	2	1	162	1	164	2.42e-56	175
MS.gene01270.t1	MTR_2g010650	50.000	162	75	3	6	162	5	165	1.90e-54	169
MS.gene01270.t1	MTR_2g010630	53.061	147	61	4	23	162	20	165	2.68e-51	161
MS.gene01270.t1	MTR_2g010600	49.333	150	72	3	17	162	13	162	5.91e-51	160
MS.gene01270.t1	MTR_4g050762	50.694	144	67	3	23	162	19	162	4.48e-50	159
MS.gene01270.t1	MTR_2g012370	50.694	144	67	3	23	162	19	162	4.48e-50	159
MS.gene01270.t1	MTR_2g010690	43.827	162	80	4	7	162	8	164	1.69e-45	147
MS.gene01270.t1	MTR_2g010670	43.210	162	83	4	9	162	4	164	2.60e-44	144
MS.gene01270.t1	MTR_2g010640	43.750	160	80	4	8	162	9	163	1.90e-43	141
MS.gene01270.t1	MTR_2g010700	44.000	150	73	5	23	162	20	168	4.43e-41	135
MS.gene01270.t1	MTR_2g010610	46.479	142	52	4	23	162	19	138	7.62e-40	131
MS.gene01270.t1	MTR_4g128750	47.518	141	71	3	25	162	33	173	4.73e-37	125
MS.gene01270.t1	MTR_4g128770	44.526	137	70	3	32	162	70	206	7.61e-33	115
MS.gene01270.t1	MTR_1g067290	39.259	135	77	3	32	161	73	207	1.46e-27	102
MS.gene01270.t1	MTR_8g078770	37.349	166	88	7	8	162	12	172	3.42e-27	100
MS.gene01270.t1	MTR_5g018770	33.708	178	91	7	11	162	9	185	2.63e-24	93.6
MS.gene01270.t1	MTR_4g092515	49.367	79	39	1	8	86	9	86	5.96e-22	85.9
MS.gene01270.t1	MTR_5g018755	37.079	89	50	4	80	162	15	103	9.91e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01270.t1	AT4G33720	54.037	161	70	2	3	162	6	163	3.46e-59	181
MS.gene01270.t1	AT2G14610	53.289	152	70	1	11	162	11	161	8.66e-57	175
MS.gene01270.t1	AT2G14580	51.299	154	72	2	11	162	9	161	1.16e-56	175
MS.gene01270.t1	AT2G19990	49.697	165	74	3	7	162	12	176	3.09e-56	174
MS.gene01270.t1	AT3G19690	51.049	143	65	2	24	162	20	161	9.29e-51	160
MS.gene01270.t1	AT1G50060	49.390	164	75	3	1	162	4	161	1.17e-50	160
MS.gene01270.t1	AT5G26130	48.485	165	80	3	1	162	4	166	2.23e-50	159
MS.gene01270.t1	AT4G33730	49.351	154	69	2	18	162	19	172	3.47e-47	151
MS.gene01270.t1	AT4G33710	51.064	141	66	2	25	162	26	166	1.89e-46	149
MS.gene01270.t1	AT4G25790	48.148	135	67	3	31	162	76	210	7.66e-40	134
MS.gene01270.t1	AT4G30320	42.073	164	90	3	1	162	1	161	4.74e-39	130
MS.gene01270.t1	AT4G07820	46.763	139	71	3	25	162	24	160	4.97e-39	130
MS.gene01270.t1	AT1G50050	44.848	165	82	4	1	162	4	162	1.19e-38	129
MS.gene01270.t1	AT1G01310	48.872	133	66	2	32	162	87	219	1.31e-38	132
MS.gene01270.t1	AT5G57625	48.120	133	66	3	33	162	75	207	4.99e-38	129
MS.gene01270.t1	AT4G31470	44.776	134	72	2	31	162	52	185	2.81e-36	124
MS.gene01270.t1	AT2G19970	43.750	144	69	5	30	162	35	177	2.98e-36	124
MS.gene01270.t1	AT3G09590	40.411	146	83	3	21	162	41	186	4.64e-35	121
MS.gene01270.t1	AT1G50050	42.331	163	85	4	1	160	4	160	1.08e-34	121
MS.gene01270.t1	AT5G02730	42.466	146	80	3	21	162	48	193	2.71e-34	119
MS.gene01270.t1	AT4G25780	41.772	158	82	5	11	162	37	190	1.45e-33	117
MS.gene01270.t1	AT5G66590	42.553	141	74	5	29	162	45	185	6.63e-32	113
MS.gene01270.t1	AT2G19980	40.120	167	74	7	13	162	8	165	1.37e-31	111

Find 41 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCATTATACTCAAATTATT+TGG	0.165449	2.2:-70927740	MS.gene01270:CDS
TGGTGTTGGTCCACTTTATT+GGG	0.256190	2.2:-70927964	MS.gene01270:CDS
TTGGTGTTGGTCCACTTTAT+TGG	0.259570	2.2:-70927965	MS.gene01270:CDS
CGTTCTCCTTCAACATTTCC+AGG	0.274248	2.2:+70927639	None:intergenic
AACCCAACCATTCTTACATT+TGG	0.275936	2.2:+70927686	None:intergenic
TTAGAGTGATAATCATAATT+AGG	0.290359	2.2:+70927786	None:intergenic
GTACGGATTCTGTGAAGTTT+TGG	0.310490	2.2:-70927821	MS.gene01270:CDS
TTGTGAGCTTGAGCACTCTA+TGG	0.317138	2.2:-70927889	MS.gene01270:CDS
GAGCATAGGCTTCAAGGTTA+TGG	0.354779	2.2:+70927940	None:intergenic
GGTTGAGCAAATGAAGAAAA+TGG	0.360878	2.2:+70928067	None:intergenic
GGGAAATATTAATGCAACTA+AGG	0.364039	2.2:+70928045	None:intergenic
GAGCACTCTATGGGTCCTTA+TGG	0.400158	2.2:-70927879	MS.gene01270:CDS
TGGCGTGATTCAGTTCATCT+TGG	0.443876	2.2:-70927720	MS.gene01270:CDS
GAGAACCTTGCTGAGGGTTA+TGG	0.444830	2.2:-70927855	MS.gene01270:CDS
TATATAAAAGAAAATCAATA+TGG	0.450206	2.2:+70927615	None:intergenic
AGTCCAAATGTAAGAATGGT+TGG	0.453283	2.2:-70927689	MS.gene01270:CDS
GTCCAAATGTAAGAATGGTT+GGG	0.466932	2.2:-70927688	MS.gene01270:CDS
TCACCACCTGGAAATGTTGA+AGG	0.498349	2.2:-70927645	MS.gene01270:CDS
GTACCTCAAGGTAATCTTGA+GGG	0.504079	2.2:+70928009	None:intergenic
GGAAATATTAATGCAACTAA+GGG	0.514988	2.2:+70928046	None:intergenic
TCAGCAAGGTTCTCACCATA+AGG	0.519070	2.2:+70927864	None:intergenic
AGGTTATGGTCCCAATAAAG+TGG	0.549917	2.2:+70927954	None:intergenic
TGTGAGCTTGAGCACTCTAT+GGG	0.560190	2.2:-70927888	MS.gene01270:CDS
TTATGGTGAGAACCTTGCTG+AGG	0.564497	2.2:-70927862	MS.gene01270:CDS
ATGGCTAAGATCATATTATG+TGG	0.564871	2.2:+70928086	None:intergenic
GCTCGAGATGAGGTTGGTGT+TGG	0.566361	2.2:-70927978	MS.gene01270:CDS
GCTAAGTCCAAATGTAAGAA+TGG	0.566456	2.2:-70927693	MS.gene01270:CDS
ATTTGTAGCTATTCACCACC+TGG	0.588741	2.2:-70927657	MS.gene01270:CDS
GAGGGTTATGGTGAAGTGAA+TGG	0.598130	2.2:-70927843	MS.gene01270:CDS
TTATGGTGAAGTGAATGGTA+CGG	0.605852	2.2:-70927838	MS.gene01270:CDS
GAGCTTGATTATGTACCTCA+AGG	0.611826	2.2:+70927997	None:intergenic
TATGGTGAGAACCTTGCTGA+GGG	0.618070	2.2:-70927861	MS.gene01270:CDS
AATCAAGCTCGAGATGAGGT+TGG	0.619159	2.2:-70927984	MS.gene01270:CDS
TGTACCTCAAGGTAATCTTG+AGG	0.625790	2.2:+70928008	None:intergenic
TACCTCAAGGTAATCTTGAG+GGG	0.626699	2.2:+70928010	None:intergenic
TCTTGTGTTAATGATGAGTG+TGG	0.652987	2.2:-70927762	MS.gene01270:CDS
ACATAATCAAGCTCGAGATG+AGG	0.662626	2.2:-70927988	MS.gene01270:CDS
CTTCACCATAACCCTCAGCA+AGG	0.671746	2.2:+70927850	None:intergenic
TCTCCTTCAACATTTCCAGG+TGG	0.683307	2.2:+70927642	None:intergenic
CTTGTGTTAATGATGAGTGT+GGG	0.697366	2.2:-70927761	MS.gene01270:CDS
CTCCCCTCAAGATTACCTTG+AGG	0.725233	2.2:-70928012	MS.gene01270:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAGAGTGATAATCATAATT+AGG	+	chr2.2:70927941-70927960	None:intergenic	20.0%
!	GGAAATATTAATGCAACTAA+GGG	+	chr2.2:70927681-70927700	None:intergenic	25.0%
!	GGCATTATACTCAAATTATT+TGG	-	chr2.2:70927984-70928003	MS.gene01270:CDS	25.0%
	ATGGCTAAGATCATATTATG+TGG	+	chr2.2:70927641-70927660	None:intergenic	30.0%
	GGGAAATATTAATGCAACTA+AGG	+	chr2.2:70927682-70927701	None:intergenic	30.0%
	AACCCAACCATTCTTACATT+TGG	+	chr2.2:70928041-70928060	None:intergenic	35.0%
	AGTCCAAATGTAAGAATGGT+TGG	-	chr2.2:70928035-70928054	MS.gene01270:CDS	35.0%
	CTTGTGTTAATGATGAGTGT+GGG	-	chr2.2:70927963-70927982	MS.gene01270:CDS	35.0%
	GCTAAGTCCAAATGTAAGAA+TGG	-	chr2.2:70928031-70928050	MS.gene01270:CDS	35.0%
	GGTTGAGCAAATGAAGAAAA+TGG	+	chr2.2:70927660-70927679	None:intergenic	35.0%
	GTCCAAATGTAAGAATGGTT+GGG	-	chr2.2:70928036-70928055	MS.gene01270:CDS	35.0%
	TCTTGTGTTAATGATGAGTG+TGG	-	chr2.2:70927962-70927981	MS.gene01270:CDS	35.0%
	TTATGGTGAAGTGAATGGTA+CGG	-	chr2.2:70927886-70927905	MS.gene01270:CDS	35.0%
!!!	GTTTGCATAGTTTTGAGCAT+AGG	+	chr2.2:70927801-70927820	None:intergenic	35.0%
	ACATAATCAAGCTCGAGATG+AGG	-	chr2.2:70927736-70927755	MS.gene01270:CDS	40.0%
	AGGTTATGGTCCCAATAAAG+TGG	+	chr2.2:70927773-70927792	None:intergenic	40.0%
	ATTTGTAGCTATTCACCACC+TGG	-	chr2.2:70928067-70928086	MS.gene01270:CDS	40.0%
	GAGCTTGATTATGTACCTCA+AGG	+	chr2.2:70927730-70927749	None:intergenic	40.0%
	GTACCTCAAGGTAATCTTGA+GGG	+	chr2.2:70927718-70927737	None:intergenic	40.0%
	TACCTCAAGGTAATCTTGAG+GGG	+	chr2.2:70927717-70927736	None:intergenic	40.0%
	TGTACCTCAAGGTAATCTTG+AGG	+	chr2.2:70927719-70927738	None:intergenic	40.0%
!	GTACGGATTCTGTGAAGTTT+TGG	-	chr2.2:70927903-70927922	MS.gene01270:CDS	40.0%
!!	TGGTGTTGGTCCACTTTATT+GGG	-	chr2.2:70927760-70927779	MS.gene01270:CDS	40.0%
!!	TTGGTGTTGGTCCACTTTAT+TGG	-	chr2.2:70927759-70927778	MS.gene01270:CDS	40.0%
!!!	AGTTTTGAGCATAGGCTTCA+AGG	+	chr2.2:70927793-70927812	None:intergenic	40.0%
	AATCAAGCTCGAGATGAGGT+TGG	-	chr2.2:70927740-70927759	MS.gene01270:CDS	45.0%
	CGTTCTCCTTCAACATTTCC+AGG	+	chr2.2:70928088-70928107	None:intergenic	45.0%
	GAGCATAGGCTTCAAGGTTA+TGG	+	chr2.2:70927787-70927806	None:intergenic	45.0%
	GAGGGTTATGGTGAAGTGAA+TGG	-	chr2.2:70927881-70927900	MS.gene01270:CDS	45.0%
	TATGGTGAGAACCTTGCTGA+GGG	-	chr2.2:70927863-70927882	MS.gene01270:CDS	45.0%
	TCACCACCTGGAAATGTTGA+AGG	-	chr2.2:70928079-70928098	MS.gene01270:CDS	45.0%
	TCAGCAAGGTTCTCACCATA+AGG	+	chr2.2:70927863-70927882	None:intergenic	45.0%
	TCTCCTTCAACATTTCCAGG+TGG	+	chr2.2:70928085-70928104	None:intergenic	45.0%
	TGGCGTGATTCAGTTCATCT+TGG	-	chr2.2:70928004-70928023	MS.gene01270:CDS	45.0%
	TGTGAGCTTGAGCACTCTAT+GGG	-	chr2.2:70927836-70927855	MS.gene01270:CDS	45.0%
	TTATGGTGAGAACCTTGCTG+AGG	-	chr2.2:70927862-70927881	MS.gene01270:CDS	45.0%
	TTGTGAGCTTGAGCACTCTA+TGG	-	chr2.2:70927835-70927854	MS.gene01270:CDS	45.0%
!!	TTGAGGGGAGTTTTGTGCTT+GGG	+	chr2.2:70927702-70927721	None:intergenic	45.0%
	CTCCCCTCAAGATTACCTTG+AGG	-	chr2.2:70927712-70927731	MS.gene01270:CDS	50.0%
	CTTCACCATAACCCTCAGCA+AGG	+	chr2.2:70927877-70927896	None:intergenic	50.0%
	GAGAACCTTGCTGAGGGTTA+TGG	-	chr2.2:70927869-70927888	MS.gene01270:CDS	50.0%
	GAGCACTCTATGGGTCCTTA+TGG	-	chr2.2:70927845-70927864	MS.gene01270:CDS	50.0%
!!	CTTGAGGGGAGTTTTGTGCT+TGG	+	chr2.2:70927703-70927722	None:intergenic	50.0%
!!	GCTCGAGATGAGGTTGGTGT+TGG	-	chr2.2:70927746-70927765	MS.gene01270:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	70927629	70928117	70927629	ID=MS.gene01270
chr2.2	mRNA	70927629	70928117	70927629	ID=MS.gene01270.t1;Parent=MS.gene01270
chr2.2	exon	70927629	70928117	70927629	ID=MS.gene01270.t1.exon1;Parent=MS.gene01270.t1
chr2.2	CDS	70927629	70928117	70927629	ID=cds.MS.gene01270.t1;Parent=MS.gene01270.t1

Gene Sequence

>MS.gene01270

ATGAGGTCTCCACATAATATGATCTTAGCCATTTTCTTCATTTGCTCAACCCTTAGTTGCATTAATATTTCCCAAGCACAAAACTCCCCTCAAGATTACCTTGAGGTACATAATCAAGCTCGAGATGAGGTTGGTGTTGGTCCACTTTATTGGGACCATAACCTTGAAGCCTATGCTCAAAACTATGCAAACAAGAGAATTAAAGATTGTGAGCTTGAGCACTCTATGGGTCCTTATGGTGAGAACCTTGCTGAGGGTTATGGTGAAGTGAATGGTACGGATTCTGTGAAGTTTTGGCTAAGTGAAAAGCCTAATTATGATTATCACTCTAACTCTTGTGTTAATGATGAGTGTGGGCATTATACTCAAATTATTTGGCGTGATTCAGTTCATCTTGGTTGTGCTAAGTCCAAATGTAAGAATGGTTGGGTTTTTGTCATTTGTAGCTATTCACCACCTGGAAATGTTGAAGGAGAACGACCATATTGA

Protein sequence

>MS.gene01270.t1

MRSPHNMILAIFFICSTLSCINISQAQNSPQDYLEVHNQARDEVGVGPLYWDHNLEAYAQNYANKRIKDCELEHSMGPYGENLAEGYGEVNGTDSVKFWLSEKPNYDYHSNSCVNDECGHYTQIIWRDSVHLGCAKSKCKNGWVFVICSYSPPGNVEGERPY