AlfalfaGEDB | MS.gene002296

M. sativa cultivar XinJiangDaYe / MS.gene002296

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002296.t1	RHN71757.1	86.4	162	19	2	1	159	274	435	1.70E-75	292

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002296.t1	Q9ZNS4	71.0	138	38	1	22	159	26	161	3.8e-55	215.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002296.t1	G7IFD1	86.4	162	19	2	1	159	1	162	1.2e-75	292.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene01356	MS.gene002296	PPI
MS.gene002297	MS.gene002296	PPI
MS.gene01273	MS.gene002296	PPI
MS.gene002292	MS.gene002296	PPI
MS.gene01686	MS.gene002296	PPI
MS.gene012420	MS.gene002296	PPI
MS.gene002295	MS.gene002296	PPI
MS.gene02252	MS.gene002296	PPI
MS.gene038936	MS.gene002296	PPI
MS.gene035685	MS.gene002296	PPI
MS.gene01685	MS.gene002296	PPI
MS.gene02249	MS.gene002296	PPI
MS.gene01275	MS.gene002296	PPI
MS.gene002294	MS.gene002296	PPI
MS.gene01270	MS.gene002296	PPI
MS.gene01687	MS.gene002296	PPI
MS.gene01277	MS.gene002296	PPI
MS.gene002293	MS.gene002296	PPI
MS.gene035687	MS.gene002296	PPI
MS.gene01690	MS.gene002296	PPI
MS.gene01684	MS.gene002296	PPI
MS.gene02258	MS.gene002296	PPI
MS.gene00249	MS.gene002296	PPI
MS.gene028545	MS.gene002296	PPI
MS.gene02254	MS.gene002296	PPI
MS.gene018353	MS.gene002296	PPI
MS.gene002299	MS.gene002296	PPI
MS.gene02255	MS.gene002296	PPI
MS.gene01274	MS.gene002296	PPI
MS.gene57900	MS.gene002296	PPI
MS.gene01272	MS.gene002296	PPI
MS.gene002296	MS.gene01274	PPI
MS.gene02256	MS.gene002296	PPI
MS.gene01688	MS.gene002296	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002296.t1	MTR_2g010600	86.420	162	19	2	1	159	1	162	6.72e-100	284
MS.gene002296.t1	MTR_2g010590	79.874	159	32	0	1	159	1	159	4.88e-98	279
MS.gene002296.t1	MTR_4g050762	85.185	162	21	2	1	159	1	162	1.62e-91	264
MS.gene002296.t1	MTR_2g012370	85.185	162	21	2	1	159	1	162	1.62e-91	264
MS.gene002296.t1	MTR_2g010610	82.500	160	5	3	1	159	1	138	2.62e-80	234
MS.gene002296.t1	MTR_2g010650	68.862	167	42	6	1	159	1	165	2.79e-78	229
MS.gene002296.t1	MTR_2g010630	71.242	153	37	3	13	159	14	165	1.28e-76	225
MS.gene002296.t1	MTR_2g010700	61.538	169	54	5	1	159	1	168	2.89e-67	202
MS.gene002296.t1	MTR_2g010640	62.424	165	54	5	1	159	1	163	1.04e-65	198
MS.gene002296.t1	MTR_2g435490	54.487	156	69	2	6	159	9	164	2.74e-61	187
MS.gene002296.t1	MTR_2g010670	60.241	166	57	5	1	159	1	164	7.87e-61	186
MS.gene002296.t1	MTR_2g010690	57.831	166	61	5	1	159	1	164	2.16e-59	182
MS.gene002296.t1	MTR_2g010580	47.170	159	83	1	1	159	7	164	4.85e-54	168
MS.gene002296.t1	MTR_4g092540	49.375	160	70	3	1	159	10	159	4.81e-52	163
MS.gene002296.t1	MTR_4g128750	50.376	133	64	2	29	159	41	173	5.67e-43	140
MS.gene002296.t1	MTR_4g128770	44.526	137	71	2	28	159	70	206	1.77e-40	135
MS.gene002296.t1	MTR_1g067290	42.963	135	73	3	28	158	73	207	1.86e-34	120
MS.gene002296.t1	MTR_8g078770	35.329	167	94	5	7	159	6	172	7.35e-27	99.8
MS.gene002296.t1	MTR_4g092515	50.495	101	48	2	3	101	6	106	4.14e-26	96.7
MS.gene002296.t1	MTR_5g018770	37.857	140	78	5	28	159	47	185	2.21e-25	96.3
MS.gene002296.t1	MTR_5g018755	35.789	95	55	4	71	159	9	103	1.53e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002296.t1	AT2G14580	63.354	161	55	2	1	159	3	161	2.02e-70	209
MS.gene002296.t1	AT4G33720	61.039	154	59	1	6	159	11	163	8.34e-68	203
MS.gene002296.t1	AT2G14610	60.390	154	59	1	6	159	10	161	1.96e-64	194
MS.gene002296.t1	AT3G19690	56.410	156	64	2	6	159	8	161	1.20e-62	190
MS.gene002296.t1	AT5G26130	58.333	156	63	1	6	159	11	166	1.16e-61	187
MS.gene002296.t1	AT4G33710	60.000	140	54	1	22	159	27	166	1.62e-58	180
MS.gene002296.t1	AT1G50060	49.686	159	76	2	2	159	6	161	3.32e-51	161
MS.gene002296.t1	AT4G33730	54.545	132	58	2	29	159	42	172	3.87e-48	154
MS.gene002296.t1	AT2G19990	53.906	128	58	1	33	159	49	176	1.15e-47	152
MS.gene002296.t1	AT4G25790	51.471	136	64	2	26	159	75	210	4.78e-47	152
MS.gene002296.t1	AT5G57625	51.880	133	62	2	29	159	75	207	4.25e-46	149
MS.gene002296.t1	AT1G50050	49.375	160	76	3	2	159	6	162	2.03e-45	146
MS.gene002296.t1	AT4G30320	44.966	149	79	3	13	159	14	161	5.07e-44	143
MS.gene002296.t1	AT3G09590	47.407	135	68	2	28	159	52	186	8.48e-44	143
MS.gene002296.t1	AT4G25780	48.905	137	65	3	28	159	54	190	5.37e-43	141
MS.gene002296.t1	AT1G50050	48.101	158	77	3	2	157	6	160	3.35e-42	140
MS.gene002296.t1	AT4G07820	46.763	139	72	2	21	159	24	160	1.52e-41	136
MS.gene002296.t1	AT1G01310	45.865	133	71	1	28	159	87	219	9.92e-41	137
MS.gene002296.t1	AT5G02730	44.853	136	72	2	27	159	58	193	3.30e-40	134
MS.gene002296.t1	AT4G31470	43.704	135	75	1	26	159	51	185	2.19e-38	129
MS.gene002296.t1	AT5G66590	42.336	137	73	4	29	159	49	185	1.78e-31	111
MS.gene002296.t1	AT2G19970	41.844	141	69	6	30	159	39	177	1.93e-27	101
MS.gene002296.t1	AT2G19980	36.095	169	86	8	3	159	7	165	3.78e-23	89.7

Find 40 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCACTCCGGTGGCCGTTAC+GGG	0.273758	2.3:-72746409	MS.gene002296:CDS
TTGGGCTTATTACTCATTAT+TGG	0.280398	2.3:-72746587	MS.gene002296:CDS
ATCCACTCCGGTGGCCGTTA+CGG	0.326170	2.3:-72746410	MS.gene002296:CDS
GCCATTATACTCAGGTTGTT+TGG	0.328715	2.3:-72746268	MS.gene002296:CDS
CATAGTCTGCTTGTGAGTCT+TGG	0.335837	2.3:+72746549	None:intergenic
TTGGTGTTCAAAATATTGTT+TGG	0.341386	2.3:-72746496	MS.gene002296:CDS
AATAGTAACACATGTGCTTC+TGG	0.347660	2.3:-72746302	MS.gene002296:CDS
TATTAAGCATGCACTTAGTA+TGG	0.353177	2.3:+72746143	None:intergenic
TCCAAACAACCTGAGTATAA+TGG	0.390459	2.3:+72746267	None:intergenic
TGGTGTTCAAAATATTGTTT+GGG	0.400203	2.3:-72746495	MS.gene002296:CDS
ATAGTCTGCTTGTGAGTCTT+GGG	0.401577	2.3:+72746550	None:intergenic
TGTGTTGATTAGCATAGTCT+TGG	0.410062	2.3:+72746450	None:intergenic
CGTTATTATAGTTGCCTGGT+GGG	0.465282	2.3:+72746171	None:intergenic
GTGTTGATTAGCATAGTCTT+GGG	0.471955	2.3:+72746451	None:intergenic
TCCATTGTCGCATCTCACTT+TGG	0.482339	2.3:+72746220	None:intergenic
ATCTGAGCCACTCAAGTCTC+CGG	0.500172	2.3:+72746367	None:intergenic
CCCCGTAACGGCCACCGGAG+TGG	0.509997	2.3:+72746408	None:intergenic
GAGAATCTCGCGGGTAGCAC+CGG	0.518238	2.3:-72746386	MS.gene002296:CDS
TGGAGAAACTCACAACGTCT+TGG	0.520363	2.3:-72746248	MS.gene002296:CDS
ATTCTCCCCGTAACGGCCAC+CGG	0.528380	2.3:+72746403	None:intergenic
GCTCAGATGCAGTGAAGTTG+TGG	0.543630	2.3:-72746352	MS.gene002296:CDS
CTCAGATGCAGTGAAGTTGT+GGG	0.551141	2.3:-72746351	MS.gene002296:CDS
TCGTTATTATAGTTGCCTGG+TGG	0.573306	2.3:+72746170	None:intergenic
ACTTGCAATTACGACCCACC+AGG	0.586857	2.3:-72746185	MS.gene002296:CDS
CCACTCCGGTGGCCGTTACG+GGG	0.597515	2.3:-72746408	MS.gene002296:CDS
CCGCGAGATTCTCCCCGTAA+CGG	0.609234	2.3:+72746396	None:intergenic
CCGTTACGGGGAGAATCTCG+CGG	0.614640	2.3:-72746396	MS.gene002296:CDS
CGTTACGGGGAGAATCTCGC+GGG	0.631764	2.3:-72746395	MS.gene002296:CDS
AACGAAGCAAGATCTGAGGT+TGG	0.637008	2.3:-72746515	MS.gene002296:CDS
GTCTTGGGCAAAACTAGCAA+CGG	0.641562	2.3:+72746466	None:intergenic
AGTGTGTGGCCATTATACTC+AGG	0.651003	2.3:-72746276	MS.gene002296:CDS
GGTAGCACCGGAGACTTGAG+TGG	0.654200	2.3:-72746374	MS.gene002296:CDS
GCCAAAGTGAGATGCGACAA+TGG	0.655075	2.3:-72746221	MS.gene002296:CDS
TGTCAACTGATCCACTCCGG+TGG	0.664496	2.3:-72746419	MS.gene002296:CDS
AGACTATGCTAATCAACACA+AGG	0.665449	2.3:-72746447	MS.gene002296:CDS
GATTGTCAACTGATCCACTC+CGG	0.665515	2.3:-72746422	MS.gene002296:CDS
TGTGCTTCTGGTAAAGTGTG+TGG	0.690422	2.3:-72746290	MS.gene002296:CDS
TCACAACGAAGCAAGATCTG+AGG	0.699112	2.3:-72746519	MS.gene002296:CDS
GACTATGCTAATCAACACAA+GGG	0.701364	2.3:-72746446	MS.gene002296:CDS
AAAGTGAGATGCGACAATGG+AGG	0.725202	2.3:-72746218	MS.gene002296:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGGTGTTCAAAATATTGTTT+GGG	-	chr2.3:72746276-72746295	MS.gene002296:CDS	25.0%
!!	ATTTTCTCTATTGTGTTTCT+TGG	-	chr2.3:72746165-72746184	MS.gene002296:CDS	25.0%
!!	TTGGTGTTCAAAATATTGTT+TGG	-	chr2.3:72746275-72746294	MS.gene002296:CDS	25.0%
!!	TTTTCTCTATTGTGTTTCTT+GGG	-	chr2.3:72746166-72746185	MS.gene002296:CDS	25.0%
	TTGGGCTTATTACTCATTAT+TGG	-	chr2.3:72746184-72746203	MS.gene002296:CDS	30.0%
!	TTTTCGTTATTATAGTTGCC+TGG	+	chr2.3:72746607-72746626	None:intergenic	30.0%
	AATAGTAACACATGTGCTTC+TGG	-	chr2.3:72746469-72746488	MS.gene002296:CDS	35.0%
	AGACTATGCTAATCAACACA+AGG	-	chr2.3:72746324-72746343	MS.gene002296:CDS	35.0%
	GACTATGCTAATCAACACAA+GGG	-	chr2.3:72746325-72746344	MS.gene002296:CDS	35.0%
	TCCAAACAACCTGAGTATAA+TGG	+	chr2.3:72746507-72746526	None:intergenic	35.0%
!!	GTGTTGATTAGCATAGTCTT+GGG	+	chr2.3:72746323-72746342	None:intergenic	35.0%
!!	TGTGTTGATTAGCATAGTCT+TGG	+	chr2.3:72746324-72746343	None:intergenic	35.0%
	ATAGTCTGCTTGTGAGTCTT+GGG	+	chr2.3:72746224-72746243	None:intergenic	40.0%
	CGTTATTATAGTTGCCTGGT+GGG	+	chr2.3:72746603-72746622	None:intergenic	40.0%
	TCGTTATTATAGTTGCCTGG+TGG	+	chr2.3:72746604-72746623	None:intergenic	40.0%
!	GCCATTATACTCAGGTTGTT+TGG	-	chr2.3:72746503-72746522	MS.gene002296:CDS	40.0%
	AAAGTGAGATGCGACAATGG+AGG	-	chr2.3:72746553-72746572	MS.gene002296:CDS	45.0%
	AGTGTGTGGCCATTATACTC+AGG	-	chr2.3:72746495-72746514	MS.gene002296:CDS	45.0%
	CATAGTCTGCTTGTGAGTCT+TGG	+	chr2.3:72746225-72746244	None:intergenic	45.0%
	CTCAGATGCAGTGAAGTTGT+GGG	-	chr2.3:72746420-72746439	MS.gene002296:CDS	45.0%
	GATTGTCAACTGATCCACTC+CGG	-	chr2.3:72746349-72746368	MS.gene002296:CDS	45.0%
	GTCTTGGGCAAAACTAGCAA+CGG	+	chr2.3:72746308-72746327	None:intergenic	45.0%
	TCACAACGAAGCAAGATCTG+AGG	-	chr2.3:72746252-72746271	MS.gene002296:CDS	45.0%
	TCCATTGTCGCATCTCACTT+TGG	+	chr2.3:72746554-72746573	None:intergenic	45.0%
	TGGAGAAACTCACAACGTCT+TGG	-	chr2.3:72746523-72746542	MS.gene002296:CDS	45.0%
	TGTGCTTCTGGTAAAGTGTG+TGG	-	chr2.3:72746481-72746500	MS.gene002296:CDS	45.0%
!	AACGAAGCAAGATCTGAGGT+TGG	-	chr2.3:72746256-72746275	MS.gene002296:CDS	45.0%
	ACTTGCAATTACGACCCACC+AGG	-	chr2.3:72746586-72746605	MS.gene002296:CDS	50.0%
	ATCTGAGCCACTCAAGTCTC+CGG	+	chr2.3:72746407-72746426	None:intergenic	50.0%
	GCCAAAGTGAGATGCGACAA+TGG	-	chr2.3:72746550-72746569	MS.gene002296:CDS	50.0%
	GCTCAGATGCAGTGAAGTTG+TGG	-	chr2.3:72746419-72746438	MS.gene002296:CDS	50.0%
	TGTCAACTGATCCACTCCGG+TGG	-	chr2.3:72746352-72746371	MS.gene002296:CDS	55.0%
	ATCCACTCCGGTGGCCGTTA+CGG	-	chr2.3:72746361-72746380	MS.gene002296:CDS	60.0%
	ATTCTCCCCGTAACGGCCAC+CGG	+	chr2.3:72746371-72746390	None:intergenic	60.0%
	CCGCGAGATTCTCCCCGTAA+CGG	+	chr2.3:72746378-72746397	None:intergenic	60.0%
	CCGTTACGGGGAGAATCTCG+CGG	-	chr2.3:72746375-72746394	MS.gene002296:CDS	60.0%
	CGTTACGGGGAGAATCTCGC+GGG	-	chr2.3:72746376-72746395	MS.gene002296:CDS	60.0%
	GAGAATCTCGCGGGTAGCAC+CGG	-	chr2.3:72746385-72746404	MS.gene002296:CDS	60.0%
!	GGTAGCACCGGAGACTTGAG+TGG	-	chr2.3:72746397-72746416	MS.gene002296:CDS	60.0%
	TCCACTCCGGTGGCCGTTAC+GGG	-	chr2.3:72746362-72746381	MS.gene002296:CDS	65.0%
	CCACTCCGGTGGCCGTTACG+GGG	-	chr2.3:72746363-72746382	MS.gene002296:CDS	70.0%
	CCCCGTAACGGCCACCGGAG+TGG	+	chr2.3:72746366-72746385	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	72746157	72746636	72746157	ID=MS.gene002296
chr2.3	mRNA	72746157	72746636	72746157	ID=MS.gene002296.t1;Parent=MS.gene002296
chr2.3	exon	72746157	72746636	72746157	ID=MS.gene002296.t1.exon1;Parent=MS.gene002296.t1
chr2.3	CDS	72746157	72746636	72746157	ID=cds.MS.gene002296.t1;Parent=MS.gene002296.t1

Gene Sequence

>MS.gene002296

ATGAGTTCATTTTCTCTATTGTGTTTCTTGGGCTTATTACTCATTATTGGTCATGTTGCACATGCCCAAGACTCACAAGCAGACTATGTGAACGCTCACAACGAAGCAAGATCTGAGGTTGGTGTTCAAAATATTGTTTGGGACAATACCGTTGCTAGTTTTGCCCAAGACTATGCTAATCAACACAAGGGTGATTGTCAACTGATCCACTCCGGTGGCCGTTACGGGGAGAATCTCGCGGGTAGCACCGGAGACTTGAGTGGCTCAGATGCAGTGAAGTTGTGGGTGAATGAGAAAGTTGACTATGATTATAATAGTAACACATGTGCTTCTGGTAAAGTGTGTGGCCATTATACTCAGGTTGTTTGGAGAAACTCACAACGTCTTGGATGTGCCAAAGTGAGATGCGACAATGGAGGCACATTCATTACTTGCAATTACGACCCACCAGGCAACTATAATAACGAAAAGCCATACTAA

Protein sequence

>MS.gene002296.t1

MSSFSLLCFLGLLLIIGHVAHAQDSQADYVNAHNEARSEVGVQNIVWDNTVASFAQDYANQHKGDCQLIHSGGRYGENLAGSTGDLSGSDAVKLWVNEKVDYDYNSNTCASGKVCGHYTQVVWRNSQRLGCAKVRCDNGGTFITCNYDPPGNYNNEKPY